AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059787

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059787.t1 MTR_2g078930 94.866 448 23 0 1 448 1 448 0.0 839
MS.gene059787.t1 MTR_2g078960 88.616 448 41 2 1 448 1 438 0.0 760
MS.gene059787.t1 MTR_2g079020 86.384 448 55 1 1 448 1 442 0.0 756
MS.gene059787.t1 MTR_2g078970 80.580 448 75 1 1 448 1 436 0.0 708
MS.gene059787.t1 MTR_2g078990 83.396 265 44 0 184 448 1 265 8.15e-162 459
MS.gene059787.t1 MTR_6g081780 42.623 427 228 5 21 441 10 425 7.67e-115 346
MS.gene059787.t1 MTR_8g106670 41.050 419 241 1 28 446 3 415 3.80e-114 344
MS.gene059787.t1 MTR_1g108810 42.250 400 231 0 42 441 33 432 1.01e-113 343
MS.gene059787.t1 MTR_6g081410 41.920 427 231 4 21 441 10 425 2.87e-113 342
MS.gene059787.t1 MTR_1g108840 40.376 426 251 1 16 441 17 439 2.34e-109 332
MS.gene059787.t1 MTR_6g081240 43.812 404 219 4 42 441 9 408 1.11e-108 329
MS.gene059787.t1 MTR_6g081300 41.935 403 228 2 42 441 9 408 2.89e-104 318
MS.gene059787.t1 MTR_6g081400 39.558 407 245 1 43 448 15 421 5.92e-104 318
MS.gene059787.t1 MTR_2g079030 89.820 167 17 0 282 448 319 485 1.88e-103 319
MS.gene059787.t1 MTR_6g066380 41.750 400 227 2 46 441 30 427 1.01e-102 315
MS.gene059787.t1 MTR_6g081810 38.657 432 228 3 11 441 4 399 6.99e-102 312
MS.gene059787.t1 MTR_6g081310 40.528 417 222 4 42 446 9 411 6.35e-100 307
MS.gene059787.t1 MTR_2g088400 36.383 459 282 3 3 451 2 460 1.14e-97 301
MS.gene059787.t1 MTR_7g082810 39.036 415 251 1 36 448 11 425 2.42e-97 301
MS.gene059787.t1 MTR_4g048000 38.322 441 261 3 12 441 22 462 2.84e-97 302
MS.gene059787.t1 MTR_6g081390 38.861 404 238 3 43 441 12 411 1.25e-96 299
MS.gene059787.t1 MTR_6g004220 37.387 444 262 3 6 448 1 429 9.65e-96 297
MS.gene059787.t1 MTR_6g004220 37.387 444 262 3 6 448 8 436 1.92e-95 296
MS.gene059787.t1 MTR_2g088400 36.184 456 281 3 3 448 2 457 2.55e-95 296
MS.gene059787.t1 MTR_2g022980 35.507 414 265 1 37 448 40 453 3.84e-87 275
MS.gene059787.t1 MTR_1g108990 36.700 406 257 0 43 448 25 430 4.57e-86 272
MS.gene059787.t1 MTR_6g004220 36.790 405 240 3 6 409 8 397 7.06e-82 260
MS.gene059787.t1 MTR_1g109060 35.523 411 255 2 43 448 24 429 3.79e-81 259
MS.gene059787.t1 MTR_1g109060 35.523 411 255 2 43 448 24 429 5.08e-81 259
MS.gene059787.t1 MTR_5g090290 35.922 412 263 1 34 445 38 448 2.15e-79 257
MS.gene059787.t1 MTR_8g063590 35.550 391 251 1 56 445 66 456 2.05e-77 250
MS.gene059787.t1 MTR_7g082800 37.464 347 217 0 102 448 1 347 1.23e-76 245
MS.gene059787.t1 MTR_8g069470 34.500 400 261 1 42 441 49 447 4.19e-76 247
MS.gene059787.t1 MTR_7g087400 36.131 429 272 1 20 448 7 433 2.83e-74 241
MS.gene059787.t1 MTR_5g032720 35.176 398 257 1 44 441 37 433 3.06e-74 241
MS.gene059787.t1 MTR_1g101500 34.000 400 258 2 52 448 73 469 6.56e-74 242
MS.gene059787.t1 MTR_5g010830 32.500 440 279 3 24 448 34 470 1.25e-73 241
MS.gene059787.t1 MTR_5g033100 34.321 405 265 1 44 448 57 460 2.19e-73 240
MS.gene059787.t1 MTR_1g100180 32.812 448 288 4 10 448 3 446 1.05e-72 238
MS.gene059787.t1 MTR_7g087410 35.784 408 259 2 43 450 31 435 1.75e-68 226
MS.gene059787.t1 MTR_1g021740 34.171 398 261 1 44 441 36 432 2.29e-68 226
MS.gene059787.t1 MTR_5g087930 33.254 418 236 5 28 445 33 407 4.96e-68 224
MS.gene059787.t1 MTR_0039s0080 33.503 394 254 5 52 441 47 436 1.76e-67 224
MS.gene059787.t1 MTR_3g099700 33.252 412 266 2 41 444 36 446 2.72e-66 221
MS.gene059787.t1 MTR_5g032750 33.800 429 271 6 16 441 15 433 5.16e-66 220
MS.gene059787.t1 MTR_6g047640 33.937 442 279 5 11 448 2 434 6.49e-66 219
MS.gene059787.t1 MTR_5g067460 30.023 443 305 2 10 447 17 459 8.78e-66 221
MS.gene059787.t1 MTR_3g099720 32.265 437 286 3 7 441 10 438 2.65e-65 218
MS.gene059787.t1 MTR_6g047610 31.910 445 283 6 11 448 2 433 6.05e-65 217
MS.gene059787.t1 MTR_6g047590 32.346 439 289 4 11 448 2 433 1.52e-64 216
MS.gene059787.t1 MTR_6g047640 32.579 442 266 6 11 448 2 415 3.97e-64 214
MS.gene059787.t1 MTR_6g004220 36.728 324 189 3 6 328 8 316 8.08e-64 209
MS.gene059787.t1 MTR_6g027200 34.152 407 265 2 43 448 36 440 3.36e-63 213
MS.gene059787.t1 MTR_8g102430 33.577 411 245 5 42 448 22 408 8.78e-63 210
MS.gene059787.t1 MTR_7g087430 37.644 348 214 2 102 448 1 346 5.12e-62 207
MS.gene059787.t1 MTR_7g087370 33.491 424 249 3 25 448 14 404 8.68e-62 208
MS.gene059787.t1 MTR_7g087410 37.249 349 216 2 102 450 1 346 2.75e-61 205
MS.gene059787.t1 MTR_6g043810 30.861 418 277 3 43 452 23 436 6.30e-60 204
MS.gene059787.t1 MTR_6g043810 30.861 418 277 3 43 452 33 446 6.95e-60 204
MS.gene059787.t1 MTR_3g111020 29.186 442 304 4 10 447 19 455 3.31e-59 203
MS.gene059787.t1 MTR_6g088805 31.116 421 266 5 30 441 17 422 1.45e-58 200
MS.gene059787.t1 MTR_4g027920 30.693 404 272 4 43 441 156 556 6.14e-56 196
MS.gene059787.t1 MTR_6g027190 34.190 389 253 2 43 430 24 410 7.16e-56 193
MS.gene059787.t1 MTR_5g011540 31.325 415 272 4 38 445 38 446 1.89e-52 184
MS.gene059787.t1 MTR_4g051552 80.909 110 14 3 191 299 191 294 3.68e-52 178
MS.gene059787.t1 MTR_2g435930 26.379 417 289 3 42 441 18 433 4.25e-42 156
MS.gene059787.t1 MTR_5g090280 28.493 365 225 5 102 438 1 357 6.95e-36 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059787.t1 AT3G23550 59.954 432 173 0 17 448 4 435 0.0 542
MS.gene059787.t1 AT3G23560 59.410 441 176 2 9 448 5 443 0.0 541
MS.gene059787.t1 AT3G23560 59.204 402 161 2 9 409 5 404 1.91e-171 488
MS.gene059787.t1 AT1G73700 43.632 424 224 2 6 429 1 409 7.02e-110 333
MS.gene059787.t1 AT2G34360 43.961 414 224 3 29 441 12 418 3.71e-109 332
MS.gene059787.t1 AT5G52450 45.363 399 218 0 43 441 25 423 4.20e-108 329
MS.gene059787.t1 AT1G15150 39.120 432 258 2 20 446 2 433 8.05e-102 313
MS.gene059787.t1 AT1G66760 38.356 438 257 3 11 448 6 430 6.05e-98 302
MS.gene059787.t1 AT1G15170 39.278 443 256 2 7 446 4 436 6.71e-98 302
MS.gene059787.t1 AT1G66760 38.356 438 257 3 11 448 6 430 1.20e-97 302
MS.gene059787.t1 AT1G15160 37.762 429 264 1 21 446 5 433 1.73e-96 299
MS.gene059787.t1 AT2G04040 39.655 406 243 2 44 448 27 431 7.33e-95 295
MS.gene059787.t1 AT1G15180 38.391 435 255 2 10 441 8 432 4.46e-92 288
MS.gene059787.t1 AT2G04080 37.440 414 241 4 44 448 27 431 2.04e-90 283
MS.gene059787.t1 AT2G34360 38.983 413 204 1 29 441 12 376 2.77e-90 281
MS.gene059787.t1 AT1G71140 36.893 412 250 3 42 448 24 430 3.36e-90 283
MS.gene059787.t1 AT2G04080 37.438 406 252 2 44 448 27 431 4.35e-90 282
MS.gene059787.t1 AT2G04070 37.772 413 241 3 44 448 27 431 1.86e-89 281
MS.gene059787.t1 AT3G21690 34.812 451 285 3 7 448 6 456 1.97e-89 281
MS.gene059787.t1 AT2G04100 37.358 439 268 4 11 448 2 434 2.36e-89 280
MS.gene059787.t1 AT2G04100 37.358 439 268 4 11 448 2 434 2.61e-89 280
MS.gene059787.t1 AT2G04050 36.547 446 259 3 11 448 2 431 3.14e-89 280
MS.gene059787.t1 AT1G11670 37.284 405 254 0 44 448 50 454 3.75e-89 281
MS.gene059787.t1 AT1G61890 35.407 418 266 1 42 459 45 458 2.86e-86 272
MS.gene059787.t1 AT2G04090 36.219 439 273 4 11 448 2 434 9.96e-86 271
MS.gene059787.t1 AT1G61890 35.872 407 261 0 42 448 45 451 7.03e-85 270
MS.gene059787.t1 AT1G61890 35.872 407 261 0 42 448 45 451 1.31e-84 268
MS.gene059787.t1 AT1G66760 37.594 399 238 2 11 409 6 393 4.23e-84 264
MS.gene059787.t1 AT1G47530 36.927 436 270 3 13 448 8 438 5.26e-84 267
MS.gene059787.t1 AT4G21903 37.831 378 234 1 71 448 80 456 8.51e-84 267
MS.gene059787.t1 AT4G21903 37.831 378 234 1 71 448 80 456 1.47e-83 266
MS.gene059787.t1 AT1G66780 35.751 386 248 0 63 448 54 439 1.99e-83 265
MS.gene059787.t1 AT1G64820 34.383 413 263 2 40 448 25 433 1.25e-80 258
MS.gene059787.t1 AT4G21910 35.309 405 261 1 44 448 57 460 1.57e-80 258
MS.gene059787.t1 AT4G21910 35.309 405 261 1 44 448 57 460 1.57e-80 258
MS.gene059787.t1 AT3G59030 34.513 452 281 5 7 445 3 452 7.36e-80 256
MS.gene059787.t1 AT4G21910 34.815 405 263 1 44 448 55 458 1.35e-79 256
MS.gene059787.t1 AT4G21910 34.815 405 263 1 44 448 55 458 1.35e-79 256
MS.gene059787.t1 AT1G23300 36.296 405 257 1 44 448 44 447 1.30e-78 253
MS.gene059787.t1 AT1G33090 37.011 435 267 2 14 448 12 439 1.92e-78 253
MS.gene059787.t1 AT1G15170 41.694 307 179 0 140 446 11 317 3.11e-78 248
MS.gene059787.t1 AT1G15150 41.830 306 178 0 140 445 11 316 7.56e-78 247
MS.gene059787.t1 AT1G12950 35.941 409 253 5 44 449 67 469 9.55e-78 251
MS.gene059787.t1 AT3G26590 34.663 401 261 1 44 444 45 444 9.84e-78 251
MS.gene059787.t1 AT1G33110 35.172 435 275 2 14 448 12 439 1.12e-77 251
MS.gene059787.t1 AT5G38030 34.165 401 263 1 44 444 45 444 4.10e-77 249
MS.gene059787.t1 AT4G21903 36.888 347 219 0 102 448 1 347 1.05e-75 242
MS.gene059787.t1 AT1G33100 35.849 424 271 1 25 448 14 436 1.20e-74 243
MS.gene059787.t1 AT5G44050 34.396 439 278 3 3 441 8 436 1.11e-73 240
MS.gene059787.t1 AT1G47530 38.040 347 214 1 102 448 1 346 3.79e-72 233
MS.gene059787.t1 AT1G33080 34.169 439 281 3 10 448 9 439 6.83e-71 233
MS.gene059787.t1 AT1G33080 34.169 439 281 3 10 448 9 439 6.99e-71 233
MS.gene059787.t1 AT4G00350 34.230 409 265 2 38 445 82 487 6.15e-70 231
MS.gene059787.t1 AT3G26590 36.152 343 218 1 102 444 1 342 1.02e-69 227
MS.gene059787.t1 AT5G10420 35.000 400 255 2 44 441 38 434 6.50e-68 225
MS.gene059787.t1 AT5G65380 31.435 439 293 3 7 445 7 437 1.48e-66 221
MS.gene059787.t1 AT2G38510 32.039 412 271 4 43 451 5 410 4.70e-65 217
MS.gene059787.t1 AT1G33110 33.747 403 259 3 9 410 6 401 5.42e-63 209
MS.gene059787.t1 AT5G19700 30.664 437 292 3 11 441 2 433 1.61e-61 208
MS.gene059787.t1 AT3G59030 31.894 417 267 6 7 409 3 416 2.67e-61 206
MS.gene059787.t1 AT4G23030 33.820 411 265 3 42 448 30 437 3.45e-61 207
MS.gene059787.t1 AT4G25640 32.598 408 272 2 37 444 28 432 4.28e-61 206
MS.gene059787.t1 AT4G25640 32.598 408 272 2 37 444 28 432 4.41e-61 207
MS.gene059787.t1 AT4G29140 31.514 403 270 2 42 441 54 453 4.73e-61 208
MS.gene059787.t1 AT4G25640 32.598 408 272 2 37 444 28 432 1.23e-60 206
MS.gene059787.t1 AT1G66760 35.644 303 193 1 146 448 2 302 2.24e-60 201
MS.gene059787.t1 AT1G58340 30.751 413 285 1 30 441 43 455 4.05e-60 205
MS.gene059787.t1 AT5G17700 31.900 442 290 6 9 448 5 437 4.36e-60 204
MS.gene059787.t1 AT5G17700 31.900 442 290 6 9 448 5 437 4.36e-60 204
MS.gene059787.t1 AT5G52050 29.663 445 291 4 6 441 7 438 7.80e-60 204
MS.gene059787.t1 AT5G49130 30.843 415 271 3 42 441 24 437 3.94e-57 197
MS.gene059787.t1 AT1G15180 38.889 270 165 0 140 409 11 280 7.07e-57 194
MS.gene059787.t1 AT3G03620 31.409 433 286 5 19 448 16 440 1.68e-56 195
MS.gene059787.t1 AT3G03620 31.409 433 286 5 19 448 16 440 1.68e-56 195
MS.gene059787.t1 AT1G71870 29.524 420 273 4 42 441 22 438 4.25e-56 194
MS.gene059787.t1 AT4G22790 29.291 437 292 5 19 444 2 432 9.11e-52 182
MS.gene059787.t1 AT5G17700 30.769 403 268 6 9 409 5 398 4.10e-50 176
MS.gene059787.t1 AT2G04066 36.449 107 60 1 342 448 28 126 3.89e-14 70.9

Find 88 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCATTTGTCCTCCATTTC+TGG 0.007321 2.4:+19410644 None:intergenic
GTGCTACTCTTGCTAATTCT+TGG 0.196457 2.4:-19410481 MS.gene059787:CDS
TAAGACCAATTTGCATTATA+AGG 0.233144 2.4:-19406088 MS.gene059787:CDS
ATTGCATGTTTAGCTCTTTC+AGG 0.235007 2.4:+19406487 None:intergenic
CACAAGGATTTCCAATGAAT+TGG 0.272145 2.4:-19406521 MS.gene059787:CDS
ATTGCTTACATGATCACTTA+TGG 0.287008 2.4:-19406702 MS.gene059787:CDS
CAATTTCATTGATTGCAATA+TGG 0.290681 2.4:-19406824 MS.gene059787:intron
TTATGCAAGTTTGGAGTATT+GGG 0.298247 2.4:-19406898 MS.gene059787:intron
TTGCATATGGATTTGTTCAT+TGG 0.305216 2.4:-19407669 MS.gene059787:CDS
ACTATGGATATCTATGATAT+TGG 0.310139 2.4:-19407602 MS.gene059787:CDS
TCTGTCCAAGGTGTCTTATC+AGG 0.328815 2.4:-19406304 MS.gene059787:intron
TTGGTTTCTGTCATGCTTGT+TGG 0.351093 2.4:-19410530 MS.gene059787:CDS
TGGACAGGTTTGAGTTTCAT+AGG 0.351705 2.4:-19407649 MS.gene059787:CDS
TGATAGTCCTATTATCAAAA+AGG 0.356312 2.4:-19406374 MS.gene059787:CDS
TTGCAACTTCTATTTCACTA+TGG 0.356695 2.4:-19407618 MS.gene059787:CDS
ACAAAACACAAGACCAGAAA+TGG 0.359995 2.4:-19410657 MS.gene059787:CDS
CTGCCAACAACTCATGATAA+TGG 0.361188 2.4:-19410683 MS.gene059787:CDS
CCATCATTATATCAATTGTT+TGG 0.361460 2.4:-19407891 MS.gene059787:CDS
ACAAGGATTTCCAATGAATT+GGG 0.362220 2.4:-19406520 MS.gene059787:CDS
TGATACCTTATAATGCAAAT+TGG 0.372077 2.4:+19406083 None:intergenic
GAAATTGTTATTTGTGAGTC+AGG 0.374296 2.4:+19406840 None:intergenic
TTGGACAGAATCTAGTAGTA+TGG 0.375092 2.4:+19406318 None:intergenic
CTTGGTGAGCTTCAGTTAGC+TGG 0.379613 2.4:-19410503 MS.gene059787:CDS
ATTCATGTTGGAATTGCATA+TGG 0.380327 2.4:-19407682 MS.gene059787:CDS
ATTATGCAAGTTTGGAGTAT+TGG 0.381254 2.4:-19406899 MS.gene059787:intron
ACACTCTGTGGACAAGGATT+TGG 0.381964 2.4:-19407979 MS.gene059787:CDS
CATAATGCAGGGGTGGTTAG+AGG 0.384287 2.4:-19406195 MS.gene059787:intron
TTGTCTTAGGTTGGTTTAAG+TGG 0.395039 2.4:-19408012 MS.gene059787:intron
AAATCCAAGGAGACATGAAA+TGG 0.395733 2.4:+19406110 None:intergenic
TTACCCATTTCATGTCTCCT+TGG 0.412363 2.4:-19406114 MS.gene059787:CDS
CTAAAGCTGTCTGTCCTCCT+TGG 0.415938 2.4:-19406454 MS.gene059787:CDS
AATTCTTGGTTTAGTGTCAC+TGG 0.416494 2.4:-19410467 MS.gene059787:CDS
TGAAAACATGAGTTGTTGTT+TGG 0.419197 2.4:+19410595 None:intergenic
TATGGATTTGTTCATTGGAC+AGG 0.434152 2.4:-19407664 MS.gene059787:CDS
GCAGGGGTGGTTAGAGGATG+TGG 0.439484 2.4:-19406189 MS.gene059787:intron
GTCATGCTTGTTGGTCACCT+TGG 0.441677 2.4:-19410521 MS.gene059787:CDS
TGTCACTGGTGTAGCAGTTA+TGG 0.450952 2.4:-19410453 MS.gene059787:intron
CAAATGGTGGAACAAAATCT+TGG 0.453309 2.4:-19410630 MS.gene059787:CDS
TTTCCATTATCATGAGTTGT+TGG 0.465756 2.4:+19410680 None:intergenic
GACCAGAAATGGAGGACAAA+TGG 0.466040 2.4:-19410646 MS.gene059787:CDS
CAAAGAAGTTTGAGAATACA+TGG 0.472713 2.4:-19407558 MS.gene059787:CDS
TTCCAACATGAATCAATGTT+GGG 0.472995 2.4:+19407692 None:intergenic
TTCTAGCAATGTCTGGTGAT+TGG 0.478680 2.4:+19407836 None:intergenic
ATTCCAACATGAATCAATGT+TGG 0.483393 2.4:+19407691 None:intergenic
AAACTAGCTCTTCCTTCTGC+AGG 0.494520 2.4:-19407490 MS.gene059787:CDS
TTCCTTCTGCAGGAATGGTA+TGG 0.496185 2.4:-19407480 MS.gene059787:intron
TGGATATCTATGATATTGGT+AGG 0.501013 2.4:-19407598 MS.gene059787:CDS
TGAGTTGTTGGCAGTAATAG+AGG 0.501704 2.4:+19410692 None:intergenic
TGCAAAGGAATATCACATGC+TGG 0.508646 2.4:-19407956 MS.gene059787:CDS
AGCTCTTCCTTCTGCAGGAA+TGG 0.510077 2.4:-19407485 MS.gene059787:CDS
GAAGCTGTTCTAGCAATGTC+TGG 0.513096 2.4:+19407829 None:intergenic
CCAAACAATTGATATAATGA+TGG 0.515139 2.4:+19407891 None:intergenic
AGTGAAATAGAAGTTGCAAC+TGG 0.517295 2.4:+19407622 None:intergenic
ATCCCAACATTGATTCATGT+TGG 0.524947 2.4:-19407694 MS.gene059787:CDS
AGGATGTGGTTGGCAGCACT+TGG 0.524967 2.4:-19406175 MS.gene059787:CDS
CATGTTTAGCTCTTTCAGGA+TGG 0.526642 2.4:+19406491 None:intergenic
CTAGAAACACTCTGTGGACA+AGG 0.528099 2.4:-19407985 MS.gene059787:CDS
ACGCTATATGCAAATAATCC+TGG 0.537941 2.4:+19407784 None:intergenic
GCGTCTTGCAAAACTTGTTG+AGG 0.540279 2.4:-19407765 MS.gene059787:CDS
TCAAAACAAAACAGAATCCA+AGG 0.547423 2.4:+19406437 None:intergenic
GCAAATTGGTCTTAAATCCA+AGG 0.548068 2.4:+19406097 None:intergenic
TATATGAAGTTTCTTATACC+AGG 0.554707 2.4:-19407802 MS.gene059787:CDS
AATCCAAGGAGACATGAAAT+GGG 0.556157 2.4:+19406111 None:intergenic
GGGTGGTTAGAGGATGTGGT+TGG 0.574146 2.4:-19406185 MS.gene059787:CDS
GCAAAGGAATATCACATGCT+GGG 0.577298 2.4:-19407955 MS.gene059787:CDS
TACCATACCATTCCTGCAGA+AGG 0.580379 2.4:+19407478 None:intergenic
CAAGGATTTCCAATGAATTG+GGG 0.583001 2.4:-19406519 MS.gene059787:CDS
AAGTTTGAGAATACATGGAG+AGG 0.586144 2.4:-19407553 MS.gene059787:CDS
ACAATCTGTAGTAATGCCAC+TGG 0.591821 2.4:-19407731 MS.gene059787:CDS
ATACTACTAGATTCTGTCCA+AGG 0.600992 2.4:-19406316 MS.gene059787:CDS
TCAAACCTGATAAGACACCT+TGG 0.605007 2.4:+19406299 None:intergenic
ATGCTGGGAATTTATCTACA+AGG 0.607996 2.4:-19407940 MS.gene059787:CDS
CAGAAATGGAGGACAAATGG+TGG 0.611520 2.4:-19410643 MS.gene059787:CDS
TCAATGTTGGGATAGCTGAA+AGG 0.615047 2.4:+19407704 None:intergenic
TGGACAAGGATTTGGTGCAA+AGG 0.616863 2.4:-19407971 MS.gene059787:CDS
GTGGAACAAAATCTTGGACA+TGG 0.619014 2.4:-19410624 MS.gene059787:CDS
AAACACAAGACCAGAAATGG+AGG 0.620372 2.4:-19410654 MS.gene059787:CDS
ATGGTCTTAGTGCAGCTGCA+AGG 0.630564 2.4:-19406683 MS.gene059787:intron
CAATGTCTGGTGATTGGTGA+AGG 0.631557 2.4:+19407842 None:intergenic
AGTATGGAAATAGCAAGCAA+AGG 0.641984 2.4:+19406334 None:intergenic
GCAGCAAGTATCATTTCAGA+CGG 0.643990 2.4:-19410725 MS.gene059787:CDS
AAGGATTTCCAATGAATTGG+GGG 0.656984 2.4:-19406518 MS.gene059787:CDS
ACAGGTTTGAGTTTCATAGG+TGG 0.673830 2.4:-19407646 MS.gene059787:CDS
CAGCTAACTGAAGCTCACCA+AGG 0.723214 2.4:+19410504 None:intergenic
ATAGCTGAAAGGATAACCAG+TGG 0.728809 2.4:+19407715 None:intergenic
GAACAAAATCTTGGACATGG+AGG 0.742594 2.4:-19410621 MS.gene059787:CDS
GGTGCACTAGAAACACTCTG+TGG 0.762528 2.4:-19407991 MS.gene059787:CDS
AAACAAAACAGAATCCAAGG+AGG 0.770094 2.4:+19406440 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATTATACTCTATAAT+GGG + chr2.4:19407414-19407433 None:intergenic 10.0%
!! AAAATTATAATAAAGAATTG+AGG + chr2.4:19406869-19406888 None:intergenic 10.0%
!! ATTTATTTCATTATAAAAAG+TGG + chr2.4:19407528-19407547 None:intergenic 10.0%
!! AAATATTATACTCTATAATG+GGG + chr2.4:19407413-19407432 None:intergenic 15.0%
!! AATAAGTTTGTAAAAATCAT+TGG + chr2.4:19406212-19406231 None:intergenic 15.0%
!! ATAATCAATTGAAGTAAATT+TGG - chr2.4:19407816-19407835 MS.gene059787:CDS 15.0%
!! ATAGTAAGATTCATAATATA+AGG + chr2.4:19409848-19409867 None:intergenic 15.0%
!! CAAAATATTATACTCTATAA+TGG + chr2.4:19407415-19407434 None:intergenic 15.0%
!! GCATAAACATTTATATTTAT+TGG + chr2.4:19406768-19406787 None:intergenic 15.0%
!! TAATAAAACAAAAATTAGTG+AGG + chr2.4:19408124-19408143 None:intergenic 15.0%
!! TAATCAATTGAAGTAAATTT+GGG - chr2.4:19407817-19407836 MS.gene059787:CDS 15.0%
!! TATTTAACACCATATTAAAT+AGG - chr2.4:19407385-19407404 MS.gene059787:intron 15.0%
!! TCTTATATTTGATTCTTAAA+TGG + chr2.4:19407682-19407701 None:intergenic 15.0%
!!! AAATTTGTTTATATGAGAAT+TGG - chr2.4:19408426-19408445 MS.gene059787:intron 15.0%
!!! AGTAATTTCATTTGAATTTT+TGG - chr2.4:19407596-19407615 MS.gene059787:CDS 15.0%
!!! ATTGATCATTTAAGTTATTT+TGG - chr2.4:19409547-19409566 MS.gene059787:intron 15.0%
!!! ATTTTATTACTTGATTACTT+TGG - chr2.4:19406229-19406248 MS.gene059787:intron 15.0%
!!! CATTATAGAGTATAATATTT+TGG - chr2.4:19407413-19407432 MS.gene059787:intron 15.0%
!!! TATTTAATATGGTGTTAAAT+AGG + chr2.4:19407386-19407405 None:intergenic 15.0%
!!! TGATTCTTATTTTTTTTGTA+GGG - chr2.4:19407282-19407301 MS.gene059787:intron 15.0%
!!! TTGATTCTTATTTTTTTTGT+AGG - chr2.4:19407281-19407300 MS.gene059787:intron 15.0%
!! AAAACAACAAAAACAAAATG+GGG + chr2.4:19408392-19408411 None:intergenic 20.0%
!! AAAGAACATAGTTTAATCTT+GGG + chr2.4:19408733-19408752 None:intergenic 20.0%
!! ACAAAACAACAAAAACAAAA+TGG + chr2.4:19408394-19408413 None:intergenic 20.0%
!! ATGATCAACATATGTAAAAA+TGG + chr2.4:19408203-19408222 None:intergenic 20.0%
!! ATTTCAAAGGAGATTATTAA+TGG + chr2.4:19407733-19407752 None:intergenic 20.0%
!! CAAAACAACAAAAACAAAAT+GGG + chr2.4:19408393-19408412 None:intergenic 20.0%
!! CATTGAATAACTTATGTATT+TGG + chr2.4:19407169-19407188 None:intergenic 20.0%
!! TACTAGGAAAAGAAAAATTA+GGG - chr2.4:19407994-19408013 MS.gene059787:CDS 20.0%
!! TCATTGACAAAAAAATCTTA+GGG - chr2.4:19410546-19410565 MS.gene059787:CDS 20.0%
!! TTATATTAAAGAATGCTCTA+AGG - chr2.4:19407919-19407938 MS.gene059787:CDS 20.0%
!! TTCATTGACAAAAAAATCTT+AGG - chr2.4:19410545-19410564 MS.gene059787:CDS 20.0%
!! TTTCGTGCATAATTTAATAA+AGG - chr2.4:19407895-19407914 MS.gene059787:CDS 20.0%
!!! ACAAAGTTTGCTAAATTATT+AGG - chr2.4:19409379-19409398 MS.gene059787:intron 20.0%
!!! ATGTTCACATACTTATTTTA+CGG + chr2.4:19407652-19407671 None:intergenic 20.0%
!!! ATTTCATTTGAATTTTTGGT+TGG - chr2.4:19407600-19407619 MS.gene059787:CDS 20.0%
!!! GAAAGATAAACTTTTTCTTA+AGG + chr2.4:19407105-19407124 None:intergenic 20.0%
!!! TATATTAGACTTTTGATTGT+TGG - chr2.4:19409481-19409500 MS.gene059787:intron 20.0%
!!! TATTTTGTATTATGCAAGTT+TGG - chr2.4:19409871-19409890 MS.gene059787:intron 20.0%
! AAATATAAGATGCTATGTTG+TGG - chr2.4:19407692-19407711 MS.gene059787:CDS 25.0%
! AAGTGAACAAATAAAGTGAA+AGG + chr2.4:19409340-19409359 None:intergenic 25.0%
! AAGTTGTTGCAAACATTAAT+GGG + chr2.4:19407767-19407786 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG + chr2.4:19406524-19406543 None:intergenic 25.0%
! ACTATGGATATCTATGATAT+TGG - chr2.4:19409176-19409195 MS.gene059787:intron 25.0%
! AGTTTAACCTTGTAAATTCA+AGG - chr2.4:19408563-19408582 MS.gene059787:intron 25.0%
! CAAAGAACATAGTTTAATCT+TGG + chr2.4:19408734-19408753 None:intergenic 25.0%
! CATTGACAAAAAAATCTTAG+GGG - chr2.4:19410547-19410566 MS.gene059787:CDS 25.0%
! CCAAACAATTGATATAATGA+TGG + chr2.4:19408890-19408909 None:intergenic 25.0%
! CCATCATTATATCAATTGTT+TGG - chr2.4:19408887-19408906 MS.gene059787:intron 25.0%
! CTACTAGGAAAAGAAAAATT+AGG - chr2.4:19407993-19408012 MS.gene059787:CDS 25.0%
! GAAAACGTTTACAAACTTAA+AGG + chr2.4:19409685-19409666 None:intergenic 25.0%
! GTATGTTCATCATAAACTTT+AGG - chr2.4:19408176-19408195 MS.gene059787:intron 25.0%
! GTGTGACTAAAATAACTTAA+AGG - chr2.4:19409695-19409714 MS.gene059787:intron 25.0%
! TAAACCAATGAGATAAAAAG+TGG + chr2.4:19410647-19410666 None:intergenic 25.0%
! TAAGACCAATTTGCATTATA+AGG - chr2.4:19410690-19410709 MS.gene059787:CDS 25.0%
! TACTTTCTTAAATCACTACT+AGG - chr2.4:19407978-19407997 MS.gene059787:CDS 25.0%
! TATATGAAGTTTCTTATACC+AGG - chr2.4:19408976-19408995 MS.gene059787:intron 25.0%
! TCATAACGCTATACTTTATT+TGG + chr2.4:19407226-19407245 None:intergenic 25.0%
! TGATACCTTATAATGCAAAT+TGG + chr2.4:19410698-19410717 None:intergenic 25.0%
! TGATAGTCCTATTATCAAAA+AGG - chr2.4:19410404-19410423 MS.gene059787:intron 25.0%
! TTAAGTGTGATGTAACATAT+TGG + chr2.4:19409782-19409801 None:intergenic 25.0%
! TTCTTATTGGTCAAAAACAA+TGG - chr2.4:19406536-19406555 MS.gene059787:intron 25.0%
!! ACATAAGTCATTTAAGTTTG+AGG + chr2.4:19409677-19409696 None:intergenic 25.0%
!! ATTGTTGGCTAAATTACATA+TGG - chr2.4:19409496-19409515 MS.gene059787:intron 25.0%
!! CAATTTCATTGATTGCAATA+TGG - chr2.4:19409954-19409973 MS.gene059787:intron 25.0%
!! CATATAAACAAATTTTGCAG+AGG + chr2.4:19408421-19408440 None:intergenic 25.0%
!! TTGTTGGCTAAATTACATAT+GGG - chr2.4:19409497-19409516 MS.gene059787:intron 25.0%
!!! AATTTTTGGTTGGAGAAAAT+TGG - chr2.4:19407610-19407629 MS.gene059787:CDS 25.0%
!!! GTAGTTGCAGTTTATTTTAT+GGG + chr2.4:19408517-19408536 None:intergenic 25.0%
!!! TGTAGTTGCAGTTTATTTTA+TGG + chr2.4:19408518-19408537 None:intergenic 25.0%
!!! TTGAACTTTTTTATAGCACA+AGG - chr2.4:19410241-19410260 MS.gene059787:intron 25.0%
!!! TTGCTTTAAAAAGACTTAAG+AGG - chr2.4:19407027-19407046 MS.gene059787:intron 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr2.4:19406523-19406542 MS.gene059787:CDS 25.0%
!!! TTTGTTTTGATGCTTTTGTT+TGG - chr2.4:19410354-19410373 MS.gene059787:intron 25.0%
!!! TTTTGTTTGGTCATGATATA+TGG - chr2.4:19410367-19410386 MS.gene059787:intron 25.0%
AAAAATGTCTTGCATAATGC+AGG - chr2.4:19410571-19410590 MS.gene059787:CDS 30.0%
AAAACAATGGAGAGAGAAAA+AGG - chr2.4:19406549-19406568 MS.gene059787:intron 30.0%
AAAATCCCTCACAAATTCTT+TGG - chr2.4:19408040-19408059 MS.gene059787:intron 30.0%
AAAATGTCTTGCATAATGCA+GGG - chr2.4:19410572-19410591 MS.gene059787:CDS 30.0%
AAATGTTGCTCAAAGTAAAC+CGG + chr2.4:19409405-19409424 None:intergenic 30.0%
AACAAAGTCTAAATTACACC+AGG + chr2.4:19409811-19409830 None:intergenic 30.0%
AAGTTTATGATGAACATACG+TGG + chr2.4:19408175-19408194 None:intergenic 30.0%
ACAAAGTCTAAATTACACCA+GGG + chr2.4:19409810-19409829 None:intergenic 30.0%
ACAAGGATTTCCAATGAATT+GGG - chr2.4:19410258-19410277 MS.gene059787:intron 30.0%
ACTTTAAATTTCCTCGAACA+TGG - chr2.4:19408591-19408610 MS.gene059787:intron 30.0%
AGGAAAAGAAAAATTAGGGA+GGG - chr2.4:19407998-19408017 MS.gene059787:CDS 30.0%
ATTCATGTTGGAATTGCATA+TGG - chr2.4:19409096-19409115 MS.gene059787:intron 30.0%
ATTCCAACATGAATCAATGT+TGG + chr2.4:19409090-19409109 None:intergenic 30.0%
ATTGCTTACATGATCACTTA+TGG - chr2.4:19410076-19410095 MS.gene059787:intron 30.0%
ATTGTTCCTATCAACATCTA+TGG + chr2.4:19406393-19406412 None:intergenic 30.0%
ATTTATTTCACGCAAATTGG+AGG - chr2.4:19408481-19408500 MS.gene059787:intron 30.0%
CAAAGAAGTTTGAGAATACA+TGG - chr2.4:19409220-19409239 MS.gene059787:intron 30.0%
CAAGTTGTTGCAAACATTAA+TGG + chr2.4:19407768-19407787 None:intergenic 30.0%
GTCATTTATTTCACGCAAAT+TGG - chr2.4:19408478-19408497 MS.gene059787:intron 30.0%
GTTCATACCTTGAATTTACA+AGG + chr2.4:19408573-19408592 None:intergenic 30.0%
TAGGAAAAGAAAAATTAGGG+AGG - chr2.4:19407997-19408016 MS.gene059787:CDS 30.0%
TAGTGAGGAAAATTTAGAGA+GGG + chr2.4:19408109-19408128 None:intergenic 30.0%
TCAAAACAAAACAGAATCCA+AGG + chr2.4:19410344-19410363 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr2.4:19406498-19406517 None:intergenic 30.0%
TCATTTCCATAGATGTTGAT+AGG - chr2.4:19406384-19406403 MS.gene059787:CDS 30.0%
TGGATATCTATGATATTGGT+AGG - chr2.4:19409180-19409199 MS.gene059787:intron 30.0%
TTAGTGAGGAAAATTTAGAG+AGG + chr2.4:19408110-19408129 None:intergenic 30.0%
TTCCAACATGAATCAATGTT+GGG + chr2.4:19409089-19409108 None:intergenic 30.0%
TTGCAACTTCTATTTCACTA+TGG - chr2.4:19409160-19409179 MS.gene059787:intron 30.0%
TTTCCATTATCATGAGTTGT+TGG + chr2.4:19406101-19406120 None:intergenic 30.0%
! AAGCTTTTTATTCTACCAAG+TGG + chr2.4:19407258-19407277 None:intergenic 30.0%
! ATTATGCAAGTTTGGAGTAT+TGG - chr2.4:19409879-19409898 MS.gene059787:intron 30.0%
! GTTTGAGGTTTGTAACAAAT+TGG + chr2.4:19409722-19409741 None:intergenic 30.0%
! TATGGGGTCATTTAAGTTTA+AGG - chr2.4:19409514-19409533 MS.gene059787:intron 30.0%
! TCATTGATTGCAATATGGTA+TGG - chr2.4:19409959-19409978 MS.gene059787:intron 30.0%
! TGAAAACATGAGTTGTTGTT+TGG + chr2.4:19406186-19406205 None:intergenic 30.0%
! TGTTGGCTAAATTACATATG+GGG - chr2.4:19409498-19409517 MS.gene059787:intron 30.0%
! TTATGCAAGTTTGGAGTATT+GGG - chr2.4:19409880-19409899 MS.gene059787:intron 30.0%
! TTGCATATGGATTTGTTCAT+TGG - chr2.4:19409109-19409128 MS.gene059787:intron 30.0%
! TTGGTGGCAAAATTTAGTTA+CGG - chr2.4:19408059-19408078 MS.gene059787:intron 30.0%
! TTTGTTACCATTGATGCTTT+TGG + chr2.4:19407795-19407814 None:intergenic 30.0%
!! AGTTTGCTAAATTATTAGGC+CGG - chr2.4:19409383-19409402 MS.gene059787:intron 30.0%
!! ATCATCGTTTTTGTAACGTT+TGG + chr2.4:19409627-19409646 None:intergenic 30.0%
!! GAAATTGTTATTTGTGAGTC+AGG + chr2.4:19409941-19409960 None:intergenic 30.0%
!! GCAAACTCCTTTTTGATAAT+AGG + chr2.4:19410414-19410433 None:intergenic 30.0%
!! TAATTTTTCAACCATGTTCG+AGG + chr2.4:19408605-19408624 None:intergenic 30.0%
!! TGGGAAAGTACTAGTAAATT+GGG - chr2.4:19407836-19407855 MS.gene059787:CDS 30.0%
!! TTGGGAAAGTACTAGTAAAT+TGG - chr2.4:19407835-19407854 MS.gene059787:CDS 30.0%
!!! AACCACTTTTTGACAACTTT+TGG + chr2.4:19406621-19406640 None:intergenic 30.0%
!!! GAAATTTTGGTTTTCTTAGC+TGG - chr2.4:19409908-19409927 MS.gene059787:intron 30.0%
!!! GTATTGGGCTTTTGAAATTT+TGG - chr2.4:19409895-19409914 MS.gene059787:intron 30.0%
AAACAAAACAGAATCCAAGG+AGG + chr2.4:19410341-19410360 None:intergenic 35.0%
AAATCCAAGGAGACATGAAA+TGG + chr2.4:19410671-19410690 None:intergenic 35.0%
AAATGTCTTGCATAATGCAG+GGG - chr2.4:19410573-19410592 MS.gene059787:CDS 35.0%
AACAGAATCCATTCCTTCAT+AGG - chr2.4:19410134-19410153 MS.gene059787:intron 35.0%
AAGGATTTCCAATGAATTGG+GGG - chr2.4:19410260-19410279 MS.gene059787:intron 35.0%
AAGTATAGCGTTATGATGTC+CGG - chr2.4:19407230-19407249 MS.gene059787:intron 35.0%
AAGTTTGAGAATACATGGAG+AGG - chr2.4:19409225-19409244 MS.gene059787:intron 35.0%
AATCCAAGGAGACATGAAAT+GGG + chr2.4:19410670-19410689 None:intergenic 35.0%
AATGGGGAGCCTATTTAATA+TGG + chr2.4:19407397-19407416 None:intergenic 35.0%
AATTCTTGGTTTAGTGTCAC+TGG - chr2.4:19406311-19406330 MS.gene059787:CDS 35.0%
ACAAAACACAAGACCAGAAA+TGG - chr2.4:19406121-19406140 MS.gene059787:CDS 35.0%
ACAGAATCCATTCCTTCATA+GGG - chr2.4:19410135-19410154 MS.gene059787:intron 35.0%
ACGCTATATGCAAATAATCC+TGG + chr2.4:19408997-19409016 None:intergenic 35.0%
ACGTTACAAAAACGATGATG+TGG - chr2.4:19409628-19409647 MS.gene059787:intron 35.0%
AGTATGGAAATAGCAAGCAA+AGG + chr2.4:19410447-19410466 None:intergenic 35.0%
AGTGAAATAGAAGTTGCAAC+TGG + chr2.4:19409159-19409178 None:intergenic 35.0%
ATAAACTGCAACTACACAAG+AGG - chr2.4:19408522-19408541 MS.gene059787:intron 35.0%
ATACTACTAGATTCTGTCCA+AGG - chr2.4:19410462-19410481 MS.gene059787:CDS 35.0%
ATGATGAACATACGTGGAAA+TGG + chr2.4:19408169-19408188 None:intergenic 35.0%
ATGCTGGGAATTTATCTACA+AGG - chr2.4:19408838-19408857 MS.gene059787:intron 35.0%
CAAAGTCTAAATTACACCAG+GGG + chr2.4:19409809-19409828 None:intergenic 35.0%
CAAGGATTTCCAATGAATTG+GGG - chr2.4:19410259-19410278 MS.gene059787:intron 35.0%
CAATAAAATGACCCCTATGA+AGG + chr2.4:19410150-19410169 None:intergenic 35.0%
CAATGAGATAAAAAGTGGCA+AGG + chr2.4:19410642-19410661 None:intergenic 35.0%
CACAAGGATTTCCAATGAAT+TGG - chr2.4:19410257-19410276 MS.gene059787:intron 35.0%
CACGGACAATCAAAAACATA+TGG - chr2.4:19406435-19406454 MS.gene059787:CDS 35.0%
CTTTCTCTTTCATACTCACA+TGG + chr2.4:19406594-19406613 None:intergenic 35.0%
GCTGAAGTTAAAAAGAGATG+AGG + chr2.4:19410508-19410527 None:intergenic 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG - chr2.4:19406616-19406635 MS.gene059787:intron 35.0%
TTGGACAGAATCTAGTAGTA+TGG + chr2.4:19410463-19410482 None:intergenic 35.0%
! CAAATGGTGGAACAAAATCT+TGG - chr2.4:19406148-19406167 MS.gene059787:CDS 35.0%
! GTGTGTTCATTGATTAGACA+CGG - chr2.4:19406417-19406436 MS.gene059787:CDS 35.0%
! TACATGGAGAGGATTTTCAA+TGG - chr2.4:19409236-19409255 MS.gene059787:intron 35.0%
! TATGGATTTGTTCATTGGAC+AGG - chr2.4:19409114-19409133 MS.gene059787:intron 35.0%
! TGTAACGTTTGGTTCACATA+GGG + chr2.4:19409616-19409635 None:intergenic 35.0%
! TTGTAACGTTTGGTTCACAT+AGG + chr2.4:19409617-19409636 None:intergenic 35.0%
!! ATCCCAACATTGATTCATGT+TGG - chr2.4:19409084-19409103 MS.gene059787:intron 35.0%
!! ATTGCATGTTTAGCTCTTTC+AGG + chr2.4:19410294-19410313 None:intergenic 35.0%
!! CTTCATAGGGGTCATTTTAT+TGG - chr2.4:19410148-19410167 MS.gene059787:intron 35.0%
!! GCAAATTGGTCTTAAATCCA+AGG + chr2.4:19410684-19410703 None:intergenic 35.0%
!! TTGTCTTAGGTTGGTTTAAG+TGG - chr2.4:19408766-19408785 MS.gene059787:intron 35.0%
!!! CTTGCCACTTTTTATCTCAT+TGG - chr2.4:19410640-19410659 MS.gene059787:CDS 35.0%
AAACACAAGACCAGAAATGG+AGG - chr2.4:19406124-19406143 MS.gene059787:CDS 40.0%
AAACTAGAAGCTAAGTGACC+GGG + chr2.4:19409453-19409472 None:intergenic 40.0%
AAATGACCCCTATGAAGGAA+TGG + chr2.4:19410145-19410164 None:intergenic 40.0%
ACAATCTGTAGTAATGCCAC+TGG - chr2.4:19409047-19409066 MS.gene059787:intron 40.0%
ACCTAAGACAAAGAAGAGCA+AGG + chr2.4:19408757-19408776 None:intergenic 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG - chr2.4:19406559-19406578 MS.gene059787:intron 40.0%
ATAGCTGAAAGGATAACCAG+TGG + chr2.4:19409066-19409085 None:intergenic 40.0%
ATCCCTCACAAATTCTTTGG+TGG - chr2.4:19408043-19408062 MS.gene059787:intron 40.0%
CAAACTAGAAGCTAAGTGAC+CGG + chr2.4:19409454-19409473 None:intergenic 40.0%
CAGAATCCATTCCTTCATAG+GGG - chr2.4:19410136-19410155 MS.gene059787:intron 40.0%
CTGCCAACAACTCATGATAA+TGG - chr2.4:19406095-19406114 MS.gene059787:CDS 40.0%
GAAACACTCGTCATTGTAGA+TGG + chr2.4:19407362-19407381 None:intergenic 40.0%
GAACAAAATCTTGGACATGG+AGG - chr2.4:19406157-19406176 MS.gene059787:CDS 40.0%
GCAAAGGAATATCACATGCT+GGG - chr2.4:19408823-19408842 MS.gene059787:intron 40.0%
GCAGCAAGTATCATTTCAGA+CGG - chr2.4:19406053-19406072 MS.gene059787:CDS 40.0%
GTGGAACAAAATCTTGGACA+TGG - chr2.4:19406154-19406173 MS.gene059787:CDS 40.0%
TCAAACCTGATAAGACACCT+TGG + chr2.4:19410482-19410501 None:intergenic 40.0%
TCAATGTTGGGATAGCTGAA+AGG + chr2.4:19409077-19409096 None:intergenic 40.0%
TGAGTTGTTGGCAGTAATAG+AGG + chr2.4:19406089-19406108 None:intergenic 40.0%
TGCAAAGGAATATCACATGC+TGG - chr2.4:19408822-19408841 MS.gene059787:intron 40.0%
TGCCACCAAAGAATTTGTGA+GGG + chr2.4:19408048-19408067 None:intergenic 40.0%
TGCTCTTCTTTGTCTTAGGT+TGG - chr2.4:19408757-19408776 MS.gene059787:intron 40.0%
TTACCCATTTCATGTCTCCT+TGG - chr2.4:19410664-19410683 MS.gene059787:CDS 40.0%
TTCTAGCAATGTCTGGTGAT+TGG + chr2.4:19408945-19408964 None:intergenic 40.0%
TTGCCACCAAAGAATTTGTG+AGG + chr2.4:19408049-19408068 None:intergenic 40.0%
TTGGTTTCTGTCATGCTTGT+TGG - chr2.4:19406248-19406267 MS.gene059787:intron 40.0%
TTTATTCTACCAAGTGGAGC+CGG + chr2.4:19407252-19407271 None:intergenic 40.0%
! ACAGGTTTGAGTTTCATAGG+TGG - chr2.4:19409132-19409151 MS.gene059787:intron 40.0%
! GTGCTACTCTTGCTAATTCT+TGG - chr2.4:19406297-19406316 MS.gene059787:intron 40.0%
! GTTACCATTGATGCTTTTGG+AGG + chr2.4:19407792-19407811 None:intergenic 40.0%
! TGCAAACATTAATGGGCCAT+TGG + chr2.4:19407760-19407779 None:intergenic 40.0%
! TGGACAGGTTTGAGTTTCAT+AGG - chr2.4:19409129-19409148 MS.gene059787:intron 40.0%
!! CATGTTTAGCTCTTTCAGGA+TGG + chr2.4:19410290-19410309 None:intergenic 40.0%
!! CTCCTTTGAAATGTAGCCAA+TGG - chr2.4:19407741-19407760 MS.gene059787:CDS 40.0%
!! TTGTCCTCCAAAAGCATCAA+TGG - chr2.4:19407785-19407804 MS.gene059787:CDS 40.0%
AACTAGAAGCTAAGTGACCG+GGG + chr2.4:19409452-19409471 None:intergenic 45.0%
ACACTCTGTGGACAAGGATT+TGG - chr2.4:19408799-19408818 MS.gene059787:intron 45.0%
ATTTCCAATGAATTGGGGGC+AGG - chr2.4:19410264-19410283 MS.gene059787:intron 45.0%
CAATGTCTGGTGATTGGTGA+AGG + chr2.4:19408939-19408958 None:intergenic 45.0%
CACCATTTGTCCTCCATTTC+TGG + chr2.4:19406137-19406156 None:intergenic 45.0%
CAGAAATGGAGGACAAATGG+TGG - chr2.4:19406135-19406154 MS.gene059787:CDS 45.0%
CATACGTGGAAATGGTCTTC+AGG + chr2.4:19408161-19408180 None:intergenic 45.0%
CATCACACTTAAAAGACCCC+TGG - chr2.4:19409790-19409809 MS.gene059787:intron 45.0%
CTAGAAACACTCTGTGGACA+AGG - chr2.4:19408793-19408812 MS.gene059787:intron 45.0%
GAAGCTGTTCTAGCAATGTC+TGG + chr2.4:19408952-19408971 None:intergenic 45.0%
GACCAGAAATGGAGGACAAA+TGG - chr2.4:19406132-19406151 MS.gene059787:CDS 45.0%
GCCTTGCTCTTCTTTGTCTT+AGG - chr2.4:19408753-19408772 MS.gene059787:intron 45.0%
TACCATACCATTCCTGCAGA+AGG + chr2.4:19409303-19409322 None:intergenic 45.0%
TGTCACTGGTGTAGCAGTTA+TGG - chr2.4:19406325-19406344 MS.gene059787:CDS 45.0%
TTCCTTCTGCAGGAATGGTA+TGG - chr2.4:19409298-19409317 MS.gene059787:intron 45.0%
! AAACTAGCTCTTCCTTCTGC+AGG - chr2.4:19409288-19409307 MS.gene059787:intron 45.0%
! GGCCATTGGCTACATTTCAA+AGG + chr2.4:19407746-19407765 None:intergenic 45.0%
! TCTGTCCAAGGTGTCTTATC+AGG - chr2.4:19410474-19410493 MS.gene059787:CDS 45.0%
!! GCGTCTTGCAAAACTTGTTG+AGG - chr2.4:19409013-19409032 MS.gene059787:intron 45.0%
!! TGGACAAGGATTTGGTGCAA+AGG - chr2.4:19408807-19408826 MS.gene059787:intron 45.0%
!! AAACAAATAAAAAAATTATA+AGG + chr2.4:19408351-19408370 None:intergenic 5.0%
!! AATATTTATTAAAATACTAA+AGG + chr2.4:19406695-19406714 None:intergenic 5.0%
!!! TTATAAAATTTTATCTAAAT+TGG + chr2.4:19407868-19407887 None:intergenic 5.0%
ACTAGAAGCTAAGTGACCGG+GGG + chr2.4:19409451-19409470 None:intergenic 50.0%
AGCTCTTCCTTCTGCAGGAA+TGG - chr2.4:19409293-19409312 MS.gene059787:intron 50.0%
AGTGCAGCTGCAAGGTTAGT+TGG - chr2.4:19410103-19410122 MS.gene059787:intron 50.0%
AGTTGAGTTACCCAATCCCC+CGG - chr2.4:19409432-19409451 MS.gene059787:intron 50.0%
CAGCTAACTGAAGCTCACCA+AGG + chr2.4:19406277-19406296 None:intergenic 50.0%
CATAATGCAGGGGTGGTTAG+AGG - chr2.4:19410583-19410602 MS.gene059787:CDS 50.0%
CTAAAGCTGTCTGTCCTCCT+TGG - chr2.4:19410324-19410343 MS.gene059787:intron 50.0%
CTTGGTGAGCTTCAGTTAGC+TGG - chr2.4:19406275-19406294 MS.gene059787:intron 50.0%
GGTGCACTAGAAACACTCTG+TGG - chr2.4:19408787-19408806 MS.gene059787:intron 50.0%
TGTCTTGCATAATGCAGGGG+TGG - chr2.4:19410576-19410595 MS.gene059787:CDS 50.0%
TTATGATGTCCGGCTCCACT+TGG - chr2.4:19407240-19407259 MS.gene059787:intron 50.0%
! ATGGTCTTAGTGCAGCTGCA+AGG - chr2.4:19410095-19410114 MS.gene059787:intron 50.0%
! GTCATGCTTGTTGGTCACCT+TGG - chr2.4:19406257-19406276 MS.gene059787:intron 50.0%
AAGCTAAGTGACCGGGGGAT+TGG + chr2.4:19409446-19409465 None:intergenic 55.0%
AGCTAAGTGACCGGGGGATT+GGG + chr2.4:19409445-19409464 None:intergenic 55.0%
AGGATGTGGTTGGCAGCACT+TGG - chr2.4:19410603-19410622 MS.gene059787:CDS 55.0%
ATGGCCTGCCCCCAATTCAT+TGG + chr2.4:19410271-19410290 None:intergenic 55.0%
GGGGGATTGGGTAACTCAAC+TGG + chr2.4:19409433-19409452 None:intergenic 55.0%
GGGTGGTTAGAGGATGTGGT+TGG - chr2.4:19410593-19410612 MS.gene059787:CDS 55.0%
GCAGGGGTGGTTAGAGGATG+TGG - chr2.4:19410589-19410608 MS.gene059787:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 19406050 19410750 19406050 ID=MS.gene059787
chr2.4 mRNA 19406050 19410750 19406050 ID=MS.gene059787.t1;Parent=MS.gene059787
chr2.4 exon 19410454 19410750 19410454 ID=MS.gene059787.t1.exon1;Parent=MS.gene059787.t1
chr2.4 CDS 19410454 19410750 19410454 ID=cds.MS.gene059787.t1;Parent=MS.gene059787.t1
chr2.4 exon 19407481 19408025 19407481 ID=MS.gene059787.t1.exon2;Parent=MS.gene059787.t1
chr2.4 CDS 19407481 19408025 19407481 ID=cds.MS.gene059787.t1;Parent=MS.gene059787.t1
chr2.4 exon 19406825 19406911 19406825 ID=MS.gene059787.t1.exon3;Parent=MS.gene059787.t1
chr2.4 CDS 19406825 19406911 19406825 ID=cds.MS.gene059787.t1;Parent=MS.gene059787.t1
chr2.4 exon 19406684 19406740 19406684 ID=MS.gene059787.t1.exon4;Parent=MS.gene059787.t1
chr2.4 CDS 19406684 19406740 19406684 ID=cds.MS.gene059787.t1;Parent=MS.gene059787.t1
chr2.4 exon 19406305 19406543 19406305 ID=MS.gene059787.t1.exon5;Parent=MS.gene059787.t1
chr2.4 CDS 19406305 19406543 19406305 ID=cds.MS.gene059787.t1;Parent=MS.gene059787.t1
chr2.4 exon 19406050 19406207 19406050 ID=MS.gene059787.t1.exon6;Parent=MS.gene059787.t1
chr2.4 CDS 19406050 19406207 19406050 ID=cds.MS.gene059787.t1;Parent=MS.gene059787.t1
Gene Sequence

>MS.gene059787

ATGGCAGCAAGTATCATTTCAGACGGCACAGAAACACCTCTATTACTGCCAACAACTCATGATAATGGAAAACAAAACACAAGACCAGAAATGGAGGACAAATGGTGGAACAAAATCTTGGACATGGAGGAAGCCAAACAACAACTCATGTTTTCACTACCAATGATTTTTACAAACTTATTTTATTACTTGATTACTTTGGTTTCTGTCATGCTTGTTGGTCACCTTGGTGAGCTTCAGTTAGCTGGTGCTACTCTTGCTAATTCTTGGTTTAGTGTCACTGGTGTAGCAGTTATGGTAATTAATTTACAGTTTTTGCATTATTATATTTTGTTCATTTCCATAGATGTTGATAGGAACAATTCTTGTGTGTTCATTGATTAGACACGGACAATCAAAAACATATGGTACAATGTTATTAAATAGAGTAATGATATTTTGACATCCATTTGATGACAACTTTGTTGACAACCTTGTTTTTCTCACTTCTTATTGGTCAAAAACAATGGAGAGAGAAAAAGGAAGAGAGAGGATAACAACACCATGTGAGTATGAAAGAGAAAGTTGTCCAAAAGTTGTCAAAAAGTGGTTGTCAAAATATCATTTCTCTATTAAATAACTTCAACATGATATGTGATGTCTCCTTTAGTATTTTAATAAATATTGAACTCTTGATCAAAAATAAAATTCTTGAACTCTTATTGATTAATTACATCCAATAAATATAAATGTTTATGCATGAATTACTAGTAAAATGTACATTTATATGTATATATGATACAAGATTAGATATTTCTTTTGTTTACGTTAGTATTACCTCAATTCTTTATTATAATTTTTTTTTTTACTTTTTAGATTCATTGAATACTTATGTATTTGATCTATATCATAGTACATATACATTAGTTAATCAATGACTCTTATTCGATGACTTTAAAAAGTAAATTTTTTCTCATAATAAAGACGAGATAAAGTATTTGCTTTAAAAAGACTTAAGAGGTCAGTGATTTGTTTAGAGATTTGAATGATGTTAGAATATTACAACTTCGAATCCTTAAGAAAAAGTTTATCTTTCAGATTCATTGAATAATTTATGTATTCAATCTATATCATAGACCAAATACATAAGTTATTCAATGACTCTGAATAATGATATTTTTTCTTATATAAAGACCAAATAAAGTATAGCGTTATGATGTCCGGCTCCACTTGGTAGAATAAAAAGCTTGACTTGATTCTTATTTTTTTTGTAGGGATTGTCTTTATTCTTTTTGTAGCTTGTAGCTGTAGTATTTACTATACTATTAGACCATCTACAATGACGAGTGTTTCGCCTATTTAACACCATATTAAATAGGCTCCCCATTATAGAGTATAATATTTTGGTGTATAGAGAAGAGTGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATATCTATATATATATATATATATCACTCTCTCCACTTTTTATAATGAAATAAATAAATTTTGTTGCAATGTAATGATATAGAATTATTTATGTGATTTATTCAGTAATTTCATTTGAATTTTTGGTTGGAGAAAATTGGTGCTTATTAAATATTACCGTAAAATAAGTATGTGAACATAGAGAATCCATTTAAGAATCAAATATAAGATGCTATGTTGTGGATGCTCTTAGTTTCACCATTAATAATCTCCTTTGAAATGTAGCCAATGGCCCATTAATGTTTGCAACAACTTGTCCTCCAAAAGCATCAATGGTAACAAAAATAATCAATTGAAGTAAATTTGGGAAAGTACTAGTAAATTGGGAAAGTACCAATTTAGATAAAATTTTATAAAACACATTTTCGTGCATAATTTAATAAAGGATTATATTAAAGAATGCTCTAAGGACATTGTTTAAAAAAGTGCTAAAGTTTAATATAAAATACTTTCTTAAATCACTACTAGGAAAAGAAAAATTAGGGAGGGATATTAGTGACAAAAAAATAAAATCCCTCACAAATTCTTTGGTGGCAAAATTTAGTTACGGAATCAGAGAGAGATTTCACGTTTTCCCTCTCTAAATTTTCCTCACTAATTTTTGTTTTATTAGTAGTGAATAATGACCTGAAGACCATTTCCACGTATGTTCATCATAAACTTTAGGACCATTTTTACATATGTTGATCATGATATGAAAAATTCTTGTATGTGCTCATTAGACATAGTCAATTAAAAAGATAAGATATGATATGATTTTATTAATATTTAAATACGCATTTGATCGCTTCAAATATATTATTTTTCATATTTAGTCCTTATAATTTTTTTATTTGTTTTTAATTCTTGCAGATATACCCCATTTTGTTTTTGTTGTTTTGTTGAGCCTCTGCAAAATTTGTTTATATGAGAATTGGTCATTGTAATATGTGTAATTTTTATTTAAGTCATTTATTTCACGCAAATTGGAGGACTTTTTTCTCCCATAAAATAAACTGCAACTACACAAGAGGTGCTAGCTTAATAGTAAGAGTTTAACCTTGTAAATTCAAGGTATGAACTTTAAATTTCCTCGAACATGGTTGAAAAATTATCATTAGTGTCACTTTAATTAACTTATAACTGATATGAAGTCTGAATATGTAAAATTAGACGATTAACATTAAATATTCTACATGTTACAATACATAAATGTTGTCCCAAGATTAAACTATGTTCTTTGCCTTGCTCTTCTTTGTCTTAGGTTGGTTTAAGTGGTGCACTAGAAACACTCTGTGGACAAGGATTTGGTGCAAAGGAATATCACATGCTGGGAATTTATCTACAAGGCTCTTGCATTATATCTTTTATTTTTTCCATCATTATATCAATTGTTTGGTTCTACACAGAACACATTCTAGTGTTCCTTCACCAATCACCAGACATTGCTAGAACAGCTTCACTCTATATGAAGTTTCTTATACCAGGATTATTTGCATATAGCGTCTTGCAAAACTTGTTGAGGTTTCTACAAACACAATCTGTAGTAATGCCACTGGTTATCCTTTCAGCTATCCCAACATTGATTCATGTTGGAATTGCATATGGATTTGTTCATTGGACAGGTTTGAGTTTCATAGGTGGACCAGTTGCAACTTCTATTTCACTATGGATATCTATGATATTGGTAGGTTTTTATGTCTTGTATGCAAAGAAGTTTGAGAATACATGGAGAGGATTTTCAATGGAGTCATTTCAGTACTTGTATACAAACATGAAACTAGCTCTTCCTTCTGCAGGAATGGTATGGTATGAGTTTCTAAAAGCCTTTCACTTTATTTGTTCACTTTGACGATTGATCTTAGAAAACAAAGTTTGCTAAATTATTAGGCCGGTTTACTTTGAGCAACATTTTCAAACCAGTTGAGTTACCCAATCCCCCGGTCACTTAGCTTCTAGTTTGAACACTATATATTAGACTTTTGATTGTTGGCTAAATTACATATGGGGTCATTTAAGTTTAAGGTTTGTAACAGATTGATCATTTAAGTTATTTTGGTCACACATAAGTCCTTTAAGTTTGTAAACGTTTTCAATTTAATCCCTATGTGAACCAAACGTTACAAAAACGATGATGTGGTAGAAAGACTAAATCGAAAACGTTTACAAACTTAAATGACTTATGTGTGACTAAAATAACTTAAAAGACCAATTTGTTACAAACCTCAAACTTAAATGACTTATGTGTGACTAAAATAACTTAAAGGACCAATATGTTACATCACACTTAAAAGACCCCTGGTGTAATTTAGACTTTGTTGTTTAATTAAAGATCCTTATATTATGAATCTTACTATTTTTATTTTGTATTATGCAAGTTTGGAGTATTGGGCTTTTGAAATTTTGGTTTTCTTAGCTGGATTAATGCCTGACTCACAAATAACAATTTCATTGATTGCAATATGGTATGGTTCTTAATTATTATTTTGCTTAAAAAAAAAAAAGATTCATATGATTCACTATTGCTAATCTTCAAAAAATTAATGCAGTGAAAACACAGAATTCATTGCTTACATGATCACTTATGGTCTTAGTGCAGCTGCAAGGTTAGTTGGTTTAAATTAACAGAATCCATTCCTTCATAGGGGTCATTTTATTGGACTTTTCTCATGACATAAGCATTAAGCACTTTAATCTATGTTCTTGTAAATTTATTGAAAATTTTGAAACTTGAACTTTTTTATAGCACAAGGATTTCCAATGAATTGGGGGCAGGCCATCCTGAAAGAGCTAAACATGCAATGAGAGTCACTCTAAAGCTGTCTGTCCTCCTTGGATTCTGTTTTGTTTTGATGCTTTTGTTTGGTCATGATATATGGATTCAAATGTTTAGTGATAGTCCTATTATCAAAAAGGAGTTTGCTTCAATAACACCTTTGCTTGCTATTTCCATACTACTAGATTCTGTCCAAGGTGTCTTATCAGGTTTGAATTCCTCATCTCTTTTTAACTTCAGCTTTTTCTCATCAACATGTTCATTGACAAAAAAATCTTAGGGGTAAAAATGTCTTGCATAATGCAGGGGTGGTTAGAGGATGTGGTTGGCAGCACTTGGTTGTTTATGTCAACCTTGCCACTTTTTATCTCATTGGTTTACCCATTTCATGTCTCCTTGGATTTAAGACCAATTTGCATTATAAGGTATCATACTCGTTATTCTCATATAAGTGTCGCATTTGA
Protein sequence

>MS.gene059787.t1

MAASIISDGTETPLLLPTTHDNGKQNTRPEMEDKWWNKILDMEEAKQQLMFSLPMIFTNLFYYLITLVSVMLVGHLGELQLAGATLANSWFSVTGVAVMVGLSGALETLCGQGFGAKEYHMLGIYLQGSCIISFIFSIIISIVWFYTEHILVFLHQSPDIARTASLYMKFLIPGLFAYSVLQNLLRFLQTQSVVMPLVILSAIPTLIHVGIAYGFVHWTGLSFIGGPVATSISLWISMILVGFYVLYAKKFENTWRGFSMESFQYLYTNMKLALPSAGMVCLEYWAFEILVFLAGLMPDSQITISLIAICENTEFIAYMITYGLSAAASTRISNELGAGHPERAKHAMRVTLKLSVLLGFCFVLMLLFGHDIWIQMFSDSPIIKKEFASITPLLAISILLDSVQGVLSGVVRGCGWQHLVVYVNLATFYLIGLPISCLLGFKTNLHYKVSYSLFSYKCRI