Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89677.t1 | XP_003629279.1 | 99.6 | 249 | 1 | 0 | 1 | 249 | 1 | 249 | 5.90E-131 | 476.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89677.t1 | Q9SXU1 | 98.0 | 249 | 5 | 0 | 1 | 249 | 1 | 249 | 1.9e-130 | 466.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89677.t1 | G7LB82 | 99.6 | 249 | 1 | 0 | 1 | 249 | 1 | 249 | 4.2e-131 | 476.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene89677 | 0.835802 | 1.36E-56 | -1.69E-46 |
| MS.gene049653 | MS.gene89677 | 0.800166 | 1.64E-48 | -1.69E-46 |
| MS.gene049887 | MS.gene89677 | 0.823038 | 1.72E-53 | -1.69E-46 |
| MS.gene050085 | MS.gene89677 | 0.816645 | 4.97E-52 | -1.69E-46 |
| MS.gene050114 | MS.gene89677 | 0.800781 | 1.23E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene89677 | 0.869891 | 2.20E-66 | -1.69E-46 |
| MS.gene050317 | MS.gene89677 | 0.80261 | 5.17E-49 | -1.69E-46 |
| MS.gene050320 | MS.gene89677 | 0.831313 | 1.79E-55 | -1.69E-46 |
| MS.gene050386 | MS.gene89677 | 0.808215 | 3.46E-50 | -1.69E-46 |
| MS.gene050437 | MS.gene89677 | 0.854531 | 1.16E-61 | -1.69E-46 |
| MS.gene050689 | MS.gene89677 | 0.839649 | 1.40E-57 | -1.69E-46 |
| MS.gene050752 | MS.gene89677 | 0.862378 | 5.29E-64 | -1.69E-46 |
| MS.gene050838 | MS.gene89677 | 0.85717 | 1.96E-62 | -1.69E-46 |
| MS.gene050955 | MS.gene89677 | 0.829199 | 5.88E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene89677 | 0.843154 | 1.67E-58 | -1.69E-46 |
| MS.gene051063 | MS.gene89677 | 0.804884 | 1.74E-49 | -1.69E-46 |
| MS.gene051233 | MS.gene89677 | 0.818907 | 1.53E-52 | -1.69E-46 |
| MS.gene051234 | MS.gene89677 | 0.809831 | 1.56E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene89677 | 0.829359 | 5.38E-55 | -1.69E-46 |
| MS.gene051245 | MS.gene89677 | 0.85391 | 1.75E-61 | -1.69E-46 |
| MS.gene051328 | MS.gene89677 | 0.822757 | 2.00E-53 | -1.69E-46 |
| MS.gene051343 | MS.gene89677 | 0.850438 | 1.70E-60 | -1.69E-46 |
| MS.gene05134 | MS.gene89677 | 0.826589 | 2.50E-54 | -1.69E-46 |
| MS.gene051577 | MS.gene89677 | 0.800778 | 1.23E-48 | -1.69E-46 |
| MS.gene051746 | MS.gene89677 | 0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene89677 | 0.821656 | 3.60E-53 | -1.69E-46 |
| MS.gene051756 | MS.gene89677 | 0.843263 | 1.56E-58 | -1.69E-46 |
| MS.gene051757 | MS.gene89677 | 0.823007 | 1.75E-53 | -1.69E-46 |
| MS.gene05188 | MS.gene89677 | 0.807596 | 4.68E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene89677 | 0.844424 | 7.62E-59 | -1.69E-46 |
| MS.gene051975 | MS.gene89677 | 0.811876 | 5.62E-51 | -1.69E-46 |
| MS.gene052242 | MS.gene89677 | -0.806459 | 8.14E-50 | -1.69E-46 |
| MS.gene052286 | MS.gene89677 | 0.805506 | 1.29E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene89677 | 0.810537 | 1.10E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene89677 | 0.802473 | 5.52E-49 | -1.69E-46 |
| MS.gene052527 | MS.gene89677 | 0.807051 | 6.11E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene89677 | 0.842988 | 1.85E-58 | -1.69E-46 |
| MS.gene053058 | MS.gene89677 | 0.863694 | 2.07E-64 | -1.69E-46 |
| MS.gene053109 | MS.gene89677 | 0.863696 | 2.07E-64 | -1.69E-46 |
| MS.gene053590 | MS.gene89677 | 0.846238 | 2.46E-59 | -1.69E-46 |
| MS.gene053952 | MS.gene89677 | 0.824353 | 8.45E-54 | -1.69E-46 |
| MS.gene054403 | MS.gene89677 | 0.857446 | 1.62E-62 | -1.69E-46 |
| MS.gene054823 | MS.gene89677 | 0.828698 | 7.78E-55 | -1.69E-46 |
| MS.gene05517 | MS.gene89677 | 0.813735 | 2.20E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene89677 | 0.828085 | 1.09E-54 | -1.69E-46 |
| MS.gene055314 | MS.gene89677 | 0.804036 | 2.62E-49 | -1.69E-46 |
| MS.gene055424 | MS.gene89677 | 0.802507 | 5.43E-49 | -1.69E-46 |
| MS.gene055441 | MS.gene89677 | 0.829684 | 4.49E-55 | -1.69E-46 |
| MS.gene055623 | MS.gene89677 | 0.811391 | 7.17E-51 | -1.69E-46 |
| MS.gene056097 | MS.gene89677 | 0.80581 | 1.12E-49 | -1.69E-46 |
| MS.gene056181 | MS.gene89677 | 0.836791 | 7.61E-57 | -1.69E-46 |
| MS.gene056432 | MS.gene89677 | 0.806127 | 9.56E-50 | -1.69E-46 |
| MS.gene056580 | MS.gene89677 | 0.829725 | 4.38E-55 | -1.69E-46 |
| MS.gene056631 | MS.gene89677 | 0.835639 | 1.49E-56 | -1.69E-46 |
| MS.gene056760 | MS.gene89677 | 0.853089 | 3.01E-61 | -1.69E-46 |
| MS.gene056787 | MS.gene89677 | 0.853864 | 1.80E-61 | -1.69E-46 |
| MS.gene057057 | MS.gene89677 | 0.86543 | 5.94E-65 | -1.69E-46 |
| MS.gene057094 | MS.gene89677 | 0.822917 | 1.83E-53 | -1.69E-46 |
| MS.gene057140 | MS.gene89677 | 0.848134 | 7.44E-60 | -1.69E-46 |
| MS.gene057234 | MS.gene89677 | 0.868958 | 4.43E-66 | -1.69E-46 |
| MS.gene057451 | MS.gene89677 | -0.806366 | 8.52E-50 | -1.69E-46 |
| MS.gene057452 | MS.gene89677 | -0.808324 | 3.28E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene89677 | 0.812666 | 3.78E-51 | -1.69E-46 |
| MS.gene05781 | MS.gene89677 | 0.821015 | 5.05E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene89677 | 0.820803 | 5.66E-53 | -1.69E-46 |
| MS.gene058054 | MS.gene89677 | 0.822605 | 2.17E-53 | -1.69E-46 |
| MS.gene058055 | MS.gene89677 | 0.852723 | 3.83E-61 | -1.69E-46 |
| MS.gene05809 | MS.gene89677 | 0.815664 | 8.23E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene89677 | 0.804597 | 2.00E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene89677 | 0.843756 | 1.15E-58 | -1.69E-46 |
| MS.gene058639 | MS.gene89677 | 0.801547 | 8.55E-49 | -1.69E-46 |
| MS.gene058646 | MS.gene89677 | 0.841979 | 3.42E-58 | -1.69E-46 |
| MS.gene058654 | MS.gene89677 | 0.843161 | 1.66E-58 | -1.69E-46 |
| MS.gene058906 | MS.gene89677 | 0.847711 | 9.73E-60 | -1.69E-46 |
| MS.gene058976 | MS.gene89677 | 0.827084 | 1.90E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene89677 | 0.808201 | 3.48E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene89677 | 0.813402 | 2.61E-51 | -1.69E-46 |
| MS.gene05978 | MS.gene89677 | 0.806681 | 7.31E-50 | -1.69E-46 |
| MS.gene059787 | MS.gene89677 | 0.811653 | 6.29E-51 | -1.69E-46 |
| MS.gene059898 | MS.gene89677 | 0.823268 | 1.52E-53 | -1.69E-46 |
| MS.gene06001 | MS.gene89677 | 0.840818 | 6.91E-58 | -1.69E-46 |
| MS.gene06010 | MS.gene89677 | 0.804711 | 1.89E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene89677 | 0.822985 | 1.77E-53 | -1.69E-46 |
| MS.gene060296 | MS.gene89677 | 0.814022 | 1.90E-51 | -1.69E-46 |
| MS.gene060297 | MS.gene89677 | 0.814625 | 1.40E-51 | -1.69E-46 |
| MS.gene060463 | MS.gene89677 | 0.825217 | 5.29E-54 | -1.69E-46 |
| MS.gene060465 | MS.gene89677 | 0.830363 | 3.06E-55 | -1.69E-46 |
| MS.gene060563 | MS.gene89677 | 0.807629 | 4.60E-50 | -1.69E-46 |
| MS.gene060869 | MS.gene89677 | 0.806811 | 6.86E-50 | -1.69E-46 |
| MS.gene060870 | MS.gene89677 | 0.800691 | 1.28E-48 | -1.69E-46 |
| MS.gene061006 | MS.gene89677 | 0.805998 | 1.02E-49 | -1.69E-46 |
| MS.gene061145 | MS.gene89677 | 0.826321 | 2.89E-54 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89677.t1 | MTR_8g075320 | 99.598 | 249 | 1 | 0 | 1 | 249 | 1 | 249 | 0.0 | 509 |
| MS.gene89677.t1 | MTR_2g016050 | 91.667 | 228 | 19 | 0 | 1 | 228 | 1 | 228 | 7.80e-157 | 436 |
| MS.gene89677.t1 | MTR_7g070070 | 42.500 | 200 | 112 | 3 | 3 | 200 | 4 | 202 | 3.82e-53 | 172 |
| MS.gene89677.t1 | MTR_0167s0060 | 38.462 | 234 | 139 | 3 | 2 | 232 | 4 | 235 | 4.98e-53 | 171 |
| MS.gene89677.t1 | MTR_5g016590 | 42.500 | 200 | 112 | 3 | 3 | 200 | 4 | 202 | 7.55e-53 | 172 |
| MS.gene89677.t1 | MTR_1g112250 | 40.171 | 234 | 130 | 5 | 4 | 231 | 8 | 237 | 6.64e-51 | 166 |
| MS.gene89677.t1 | MTR_8g464460 | 38.428 | 229 | 133 | 5 | 4 | 225 | 9 | 236 | 3.38e-42 | 144 |
| MS.gene89677.t1 | MTR_2g060830 | 39.394 | 231 | 128 | 7 | 4 | 225 | 9 | 236 | 9.56e-42 | 143 |
| MS.gene89677.t1 | MTR_1g022410 | 37.736 | 212 | 123 | 4 | 3 | 211 | 5 | 210 | 3.08e-37 | 132 |
| MS.gene89677.t1 | MTR_6g024135 | 35.260 | 173 | 110 | 1 | 4 | 176 | 8 | 178 | 2.09e-29 | 111 |
| MS.gene89677.t1 | MTR_4g012740 | 28.685 | 251 | 130 | 6 | 3 | 211 | 236 | 479 | 4.67e-19 | 86.3 |
| MS.gene89677.t1 | MTR_4g057090 | 29.545 | 176 | 115 | 4 | 25 | 196 | 110 | 280 | 1.38e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89677.t1 | AT5G66140 | 90.400 | 250 | 23 | 1 | 1 | 249 | 1 | 250 | 4.34e-156 | 434 |
| MS.gene89677.t1 | AT3G51260 | 89.956 | 229 | 22 | 1 | 1 | 228 | 1 | 229 | 4.58e-153 | 426 |
| MS.gene89677.t1 | AT3G51260 | 91.509 | 212 | 18 | 0 | 1 | 212 | 1 | 212 | 4.47e-146 | 408 |
| MS.gene89677.t1 | AT3G22110 | 43.000 | 200 | 111 | 3 | 3 | 200 | 4 | 202 | 4.86e-54 | 175 |
| MS.gene89677.t1 | AT1G53850 | 41.453 | 234 | 127 | 5 | 4 | 231 | 8 | 237 | 3.06e-52 | 170 |
| MS.gene89677.t1 | AT1G53850 | 41.453 | 234 | 127 | 5 | 4 | 231 | 8 | 237 | 3.06e-52 | 170 |
| MS.gene89677.t1 | AT3G14290 | 42.035 | 226 | 123 | 4 | 4 | 223 | 8 | 231 | 1.17e-51 | 168 |
| MS.gene89677.t1 | AT1G16470 | 38.462 | 234 | 139 | 3 | 2 | 232 | 4 | 235 | 1.96e-51 | 167 |
| MS.gene89677.t1 | AT1G16470 | 38.462 | 234 | 139 | 3 | 2 | 232 | 4 | 235 | 1.96e-51 | 167 |
| MS.gene89677.t1 | AT1G79210 | 38.462 | 234 | 139 | 3 | 2 | 232 | 4 | 235 | 2.98e-51 | 167 |
| MS.gene89677.t1 | AT1G79210 | 38.462 | 234 | 139 | 3 | 2 | 232 | 4 | 235 | 2.98e-51 | 167 |
| MS.gene89677.t1 | AT1G79210 | 38.462 | 234 | 139 | 3 | 2 | 232 | 4 | 235 | 2.98e-51 | 167 |
| MS.gene89677.t1 | AT5G35590 | 38.627 | 233 | 133 | 4 | 2 | 230 | 7 | 233 | 1.11e-43 | 148 |
| MS.gene89677.t1 | AT2G05840 | 38.961 | 231 | 133 | 5 | 2 | 225 | 7 | 236 | 5.86e-42 | 144 |
| MS.gene89677.t1 | AT2G05840 | 38.679 | 212 | 125 | 3 | 2 | 209 | 7 | 217 | 3.07e-41 | 141 |
| MS.gene89677.t1 | AT1G47250 | 35.714 | 238 | 139 | 5 | 3 | 232 | 5 | 236 | 1.60e-39 | 138 |
| MS.gene89677.t1 | AT5G42790 | 35.294 | 238 | 140 | 5 | 3 | 232 | 5 | 236 | 3.40e-38 | 135 |
| MS.gene89677.t1 | AT2G05840 | 38.144 | 194 | 115 | 3 | 2 | 191 | 7 | 199 | 2.13e-37 | 130 |
| MS.gene89677.t1 | AT4G15165 | 34.500 | 200 | 86 | 4 | 3 | 200 | 4 | 160 | 2.62e-33 | 120 |
| MS.gene89677.t1 | AT2G27020 | 34.054 | 185 | 119 | 2 | 4 | 187 | 8 | 190 | 5.54e-30 | 112 |
| MS.gene89677.t1 | AT2G27020 | 34.054 | 185 | 119 | 2 | 4 | 187 | 110 | 292 | 1.01e-28 | 111 |
Find 66 sgRNAs with CRISPR-Local
Find 150 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACATTGCTCTTGCTTGTGC+TGG | 0.248856 | 8.3:+28245877 | MS.gene89677:CDS |
| CCAAGTCGAGTACGCTTTAG+AGG | 0.260959 | 8.3:+28244747 | MS.gene89677:CDS |
| AGTGGTGGTGTGCGGCCATT+TGG | 0.299429 | 8.3:+28246036 | MS.gene89677:CDS |
| CGGCTAAGCTTCAAGATTCT+AGG | 0.372059 | 8.3:+28244845 | MS.gene89677:CDS |
| TAAAATCATGTGTCCTTAAG+AGG | 0.395220 | 8.3:-28247243 | None:intergenic |
| CTGTACCAGACAGACCCTTC+TGG | 0.400393 | 8.3:+28246108 | MS.gene89677:CDS |
| GTGGTGGTGTGCGGCCATTT+GGG | 0.402302 | 8.3:+28246037 | MS.gene89677:CDS |
| AGTACGCTTTAGAGGCTGTT+AGG | 0.404948 | 8.3:+28244755 | MS.gene89677:CDS |
| CGACGGTGAGCCTGTGACTC+TGG | 0.410392 | 8.3:-28245948 | None:intergenic |
| ATGCTCGTGTCTTGATAAAC+CGG | 0.411856 | 8.3:+28245911 | MS.gene89677:CDS |
| GGAGCACGGTCTGCGTCAAT+TGG | 0.423486 | 8.3:+28247146 | MS.gene89677:CDS |
| CGCTTTAGAGGCTGTTAGGA+AGG | 0.448743 | 8.3:+28244759 | MS.gene89677:CDS |
| GGGCTTTCCACCTTGATCGT+TGG | 0.461708 | 8.3:+28246057 | MS.gene89677:CDS |
| ATGTACCAGAAGGGTCTGTC+TGG | 0.465812 | 8.3:-28246113 | None:intergenic |
| GGTATCGATAACGTCGTTCT+CGG | 0.466383 | 8.3:+28244805 | MS.gene89677:CDS |
| ACATTGCTCTTGCTTGTGCT+GGG | 0.466582 | 8.3:+28245878 | MS.gene89677:CDS |
| TGCAAAGAAAGCTCCTCTTA+AGG | 0.470575 | 8.3:+28247230 | MS.gene89677:CDS |
| TCTGGCATTCAACGCGTGCC+CGG | 0.479291 | 8.3:-28245930 | None:intergenic |
| CCTCTAAAGCGTACTCGACT+TGG | 0.482229 | 8.3:-28244747 | None:intergenic |
| ACTTGGAAGAGATGACCATC+GGG | 0.482902 | 8.3:-28244730 | None:intergenic |
| AACGCCGCCGTCGGTGTTCG+CGG | 0.483392 | 8.3:+28244784 | MS.gene89677:CDS |
| AGAGAGGGAGACCCGGTGTA+AGG | 0.485987 | 8.3:-28246087 | None:intergenic |
| GCATGGAAGGCAAATGCCAC+TGG | 0.502242 | 8.3:+28246141 | MS.gene89677:CDS |
| ATCAAGGTGGAAAGCCCAAA+TGG | 0.513612 | 8.3:-28246051 | None:intergenic |
| TGGACAGGAAACTGTGAAGT+TGG | 0.514356 | 8.3:+28246218 | MS.gene89677:CDS |
| AGGATCAAAGCCAACGATCA+AGG | 0.514570 | 8.3:-28246067 | None:intergenic |
| GCGTTGAATGCCAGAGTCAC+AGG | 0.514781 | 8.3:+28245938 | MS.gene89677:CDS |
| GCTGGAGACCAGCTATGTAC+CGG | 0.520483 | 8.3:-28245999 | None:intergenic |
| AACTCACTTCAAGCAAAGCT+CGG | 0.527308 | 8.3:-28246245 | None:intergenic |
| TATATTACCCGGTACATAGC+TGG | 0.536354 | 8.3:+28245991 | MS.gene89677:CDS |
| ATCGATACCGCGAACACCGA+CGG | 0.539173 | 8.3:-28244791 | None:intergenic |
| GACTTGGAAGAGATGACCAT+CGG | 0.541061 | 8.3:-28244731 | None:intergenic |
| AGCAGAGAAAGCAGCTGCTG+AGG | 0.545135 | 8.3:+28247206 | MS.gene89677:CDS |
| TGTAATTAGGTCGTCGAGAG+TGG | 0.553616 | 8.3:+28247090 | MS.gene89677:intron |
| GCACGGTCTGCGTCAATTGG+AGG | 0.557429 | 8.3:+28247149 | MS.gene89677:CDS |
| GTTGGCTTTGATCCTTACAC+CGG | 0.563925 | 8.3:+28246075 | MS.gene89677:CDS |
| GTAATATACTCCACAGTGCA+TGG | 0.569706 | 8.3:-28245976 | None:intergenic |
| AGGAAGGGTAACGCCGCCGT+CGG | 0.581917 | 8.3:+28244775 | MS.gene89677:CDS |
| CGAATTCGAGTTTCTGCCAG+TGG | 0.583434 | 8.3:-28246157 | None:intergenic |
| GCTTTAGAGGCTGTTAGGAA+GGG | 0.586935 | 8.3:+28244760 | MS.gene89677:CDS |
| CATGCTGAAAATGTACCAGA+AGG | 0.592585 | 8.3:-28246123 | None:intergenic |
| CTGGTACAGAGAGGGAGACC+CGG | 0.593324 | 8.3:-28246094 | None:intergenic |
| CTGGAGACCAGCTATGTACC+GGG | 0.601992 | 8.3:-28245998 | None:intergenic |
| CACAGTGCATGGATCTTCGA+CGG | 0.606013 | 8.3:-28245965 | None:intergenic |
| GGGTCTGTCTGGTACAGAGA+GGG | 0.610535 | 8.3:-28246102 | None:intergenic |
| CAGCAAAAGTACACACAAAG+TGG | 0.611995 | 8.3:+28246018 | MS.gene89677:CDS |
| TGAAGTTGCTGTGATGACTA+AGG | 0.614312 | 8.3:+28247125 | MS.gene89677:CDS |
| CTTCACAGTTTCCTGTCCAG+AGG | 0.615209 | 8.3:-28246214 | None:intergenic |
| TGCTCGTGTCTTGATAAACC+GGG | 0.619876 | 8.3:+28245912 | MS.gene89677:CDS |
| AGCTGAAATTGATGCCATTG+TGG | 0.626656 | 8.3:+28247173 | MS.gene89677:CDS |
| TGCTTCAATCTCAGCCACAA+TGG | 0.630980 | 8.3:-28247187 | None:intergenic |
| AGAATCTTGAAGCTTAGCCG+TGG | 0.631552 | 8.3:-28244842 | None:intergenic |
| TTGGCTTTGATCCTTACACC+GGG | 0.640792 | 8.3:+28246076 | MS.gene89677:CDS |
| ATGCTGAAAATGTACCAGAA+GGG | 0.645655 | 8.3:-28246122 | None:intergenic |
| AATTAGGTCGTCGAGAGTGG+TGG | 0.645886 | 8.3:+28247093 | MS.gene89677:intron |
| ACACACAAAGTGGTGGTGTG+CGG | 0.660974 | 8.3:+28246028 | MS.gene89677:CDS |
| GCACTGTGGAGTATATTACC+CGG | 0.676466 | 8.3:+28245980 | MS.gene89677:CDS |
| AGGGTCTGTCTGGTACAGAG+AGG | 0.681482 | 8.3:-28246103 | None:intergenic |
| CGGCGTTGAGAAGAAATCCA+CGG | 0.683184 | 8.3:+28244825 | MS.gene89677:CDS |
| CAAAAGTACACACAAAGTGG+TGG | 0.687790 | 8.3:+28246021 | MS.gene89677:CDS |
| GCTGTGATGACTAAGGAGCA+CGG | 0.689211 | 8.3:+28247132 | MS.gene89677:CDS |
| ATCAAAGCCAACGATCAAGG+TGG | 0.692772 | 8.3:-28246064 | None:intergenic |
| CGTCGAAGATCCATGCACTG+TGG | 0.702839 | 8.3:+28245966 | MS.gene89677:CDS |
| GATACCGCGAACACCGACGG+CGG | 0.718572 | 8.3:-28244788 | None:intergenic |
| CTTGGAAGAGATGACCATCG+GGG | 0.737892 | 8.3:-28244729 | None:intergenic |
| TTGGAAGAGATGACCATCGG+GGG | 0.745206 | 8.3:-28244728 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATATAATATGGATTT+GGG | + | chr8.3:28245791-28245810 | MS.gene89677:intron | 10.0% |
| !!! | TAAAAAGTAATTAAAAGTTT+TGG | - | chr8.3:28246893-28246912 | None:intergenic | 10.0% |
| !! | AAACTGCTTATAATATAATA+TGG | + | chr8.3:28245784-28245803 | MS.gene89677:intron | 15.0% |
| !! | ATATTATATTATAAGCAGTT+TGG | - | chr8.3:28245785-28245804 | None:intergenic | 15.0% |
| !! | ATTCTATCATTCATTTATAT+AGG | + | chr8.3:28246306-28246325 | MS.gene89677:intron | 15.0% |
| !! | CTTATAATATAATATGGATT+TGG | + | chr8.3:28245790-28245809 | MS.gene89677:intron | 15.0% |
| !!! | TAAGTAACTTGTTTTATATT+TGG | + | chr8.3:28247019-28247038 | MS.gene89677:intron | 15.0% |
| !! | AGAAGCTATTTATTTACATA+AGG | + | chr8.3:28245698-28245717 | MS.gene89677:intron | 20.0% |
| !! | ATTAATCGAATGAATAAACA+CGG | - | chr8.3:28246547-28246566 | None:intergenic | 20.0% |
| !! | ATTGAAATAATCACATGTTT+CGG | - | chr8.3:28245229-28245248 | None:intergenic | 20.0% |
| !! | CTCAAATGAATGTTAAAAAA+GGG | - | chr8.3:28246664-28246683 | None:intergenic | 20.0% |
| !! | GAAGCTATTTATTTACATAA+GGG | + | chr8.3:28245699-28245718 | MS.gene89677:intron | 20.0% |
| !! | TCTCAAATGAATGTTAAAAA+AGG | - | chr8.3:28246665-28246684 | None:intergenic | 20.0% |
| !! | TCTGATAAATACTTTACATT+TGG | + | chr8.3:28247049-28247068 | MS.gene89677:intron | 20.0% |
| !! | TGTACATTCAAACATTATAA+TGG | + | chr8.3:28246942-28246961 | MS.gene89677:intron | 20.0% |
| !! | TTTATTCATTCGATTAATCT+GGG | + | chr8.3:28246549-28246568 | MS.gene89677:intron | 20.0% |
| !!! | TATGACTTAGAAATTTTGTT+TGG | + | chr8.3:28244981-28245000 | MS.gene89677:intron | 20.0% |
| !!! | TGATTTTCATTTCTCAAATT+CGG | + | chr8.3:28244904-28244923 | MS.gene89677:intron | 20.0% |
| !!! | TTTATATATAGCAGCTTTAT+AGG | + | chr8.3:28245752-28245771 | MS.gene89677:intron | 20.0% |
| !!! | TTTTTTTTTTTTGTAAGACC+TGG | + | chr8.3:28245387-28245406 | MS.gene89677:intron | 20.0% |
| ! | AAATCATTTGACTGCAAATT+AGG | + | chr8.3:28246500-28246519 | MS.gene89677:intron | 25.0% |
| ! | GCATAACTGTAATACTATTA+TGG | + | chr8.3:28246991-28247010 | MS.gene89677:intron | 25.0% |
| ! | GTTTATTCATTCGATTAATC+TGG | + | chr8.3:28246548-28246567 | MS.gene89677:intron | 25.0% |
| ! | TTAATCGAATGAATAAACAC+GGG | - | chr8.3:28246546-28246565 | None:intergenic | 25.0% |
| ! | TTAGAATCTTAGATCTAGTT+TGG | + | chr8.3:28245468-28245487 | MS.gene89677:intron | 25.0% |
| !! | AAAAGCTTGTAAGATCTTTT+TGG | - | chr8.3:28245557-28245576 | None:intergenic | 25.0% |
| !! | TTTTTCTTGCTAGAAGATAT+AGG | + | chr8.3:28246353-28246372 | MS.gene89677:intron | 25.0% |
| !!! | AATTCTTTTCTAGAAACTCA+CGG | - | chr8.3:28246185-28246204 | None:intergenic | 25.0% |
| !!! | ACTTGTTTTATATTTGGAAC+TGG | + | chr8.3:28247025-28247044 | MS.gene89677:intron | 25.0% |
| !!! | GTTTTGTTGATGTTCTTTTA+TGG | + | chr8.3:28245360-28245379 | MS.gene89677:intron | 25.0% |
| TAGCAGAGGAATAGAAATAA+AGG | + | chr8.3:28245421-28245440 | MS.gene89677:intron | 30.0% | |
| TAGTATTACAGTTATGCAAC+AGG | - | chr8.3:28246988-28247007 | None:intergenic | 30.0% | |
| TATGATGTCAGTAGATAACT+TGG | + | chr8.3:28245169-28245188 | MS.gene89677:intron | 30.0% | |
| TTTATGATATTCCTAGCTGA+TGG | + | chr8.3:28246909-28246928 | MS.gene89677:intron | 30.0% | |
| ! | AAGGTTTTGCAATTTTTGTG+TGG | + | chr8.3:28245440-28245459 | MS.gene89677:intron | 30.0% |
| ! | AGATGTGAAGTAATTTGCAT+CGG | + | chr8.3:28245059-28245078 | MS.gene89677:intron | 30.0% |
| ! | GCAGTCAAATGATTTTGTTA+AGG | - | chr8.3:28246495-28246514 | None:intergenic | 30.0% |
| !! | AAGAATTTTAAAGAGACCTC+TGG | + | chr8.3:28246198-28246217 | MS.gene89677:CDS | 30.0% |
| !! | TTTTATGATGTTGACTACCT+AGG | + | chr8.3:28246389-28246408 | MS.gene89677:intron | 30.0% |
| !!! | TTTTTTTTGTAAGACCTGGT+AGG | + | chr8.3:28245391-28245410 | MS.gene89677:intron | 30.0% |
| ATGCTGAAAATGTACCAGAA+GGG | - | chr8.3:28246125-28246144 | None:intergenic | 35.0% | |
| CTTAAATCCTGCTATGAACT+GGG | + | chr8.3:28246619-28246638 | MS.gene89677:intron | 35.0% | |
| TCATAGCAGGATTTAAGGAA+TGG | - | chr8.3:28246616-28246635 | None:intergenic | 35.0% | |
| TCATTCGATTAATCTGGGTT+TGG | + | chr8.3:28246554-28246573 | MS.gene89677:intron | 35.0% | |
| TCATTTGACTGCAAATTAGG+AGG | + | chr8.3:28246503-28246522 | MS.gene89677:intron | 35.0% | |
| TGCAAATTAGGAGGAAGTTT+AGG | + | chr8.3:28246512-28246531 | MS.gene89677:intron | 35.0% | |
| TGTTGATCAACAATCAGAAG+AGG | - | chr8.3:28246693-28246712 | None:intergenic | 35.0% | |
| TTTCTATTCCTCTGCTAAAC+AGG | - | chr8.3:28245418-28245437 | None:intergenic | 35.0% | |
| ! | CTTAGGGTTTAGAGTGTAAT+CGG | + | chr8.3:28244942-28244961 | MS.gene89677:intron | 35.0% |
| ! | GTGTTAGGTTTAGGATCTTA+GGG | + | chr8.3:28244926-28244945 | MS.gene89677:intron | 35.0% |
| !! | CATTTCTCAAATTCGGTGTT+AGG | + | chr8.3:28244911-28244930 | MS.gene89677:intron | 35.0% |
| !! | TCAAATTCGGTGTTAGGTTT+AGG | + | chr8.3:28244917-28244936 | MS.gene89677:intron | 35.0% |
| !!! | AATTTGCTGCAACTTTTGCA+AGG | + | chr8.3:28246329-28246348 | MS.gene89677:intron | 35.0% |
| !!! | CTTTCGTTGTTGTTTTTCGT+TGG | + | chr8.3:28245030-28245049 | MS.gene89677:intron | 35.0% |
| !!! | TACATTTGGCTTTTCCTTGT+CGG | + | chr8.3:28247063-28247082 | MS.gene89677:intron | 35.0% |
| AACTCACTTCAAGCAAAGCT+CGG | - | chr8.3:28246248-28246267 | None:intergenic | 40.0% | |
| AAGCTTCAAGATTCTAGGTC+TGG | + | chr8.3:28244850-28244869 | MS.gene89677:intron | 40.0% | |
| ACTACAACAGTTAACACGTC+AGG | - | chr8.3:28245140-28245159 | None:intergenic | 40.0% | |
| AGCTGAAATTGATGCCATTG+TGG | + | chr8.3:28247173-28247192 | MS.gene89677:CDS | 40.0% | |
| ATATAAGCCCAGTTCATAGC+AGG | - | chr8.3:28246629-28246648 | None:intergenic | 40.0% | |
| ATGCTCGTGTCTTGATAAAC+CGG | + | chr8.3:28245911-28245930 | MS.gene89677:CDS | 40.0% | |
| ATTCCTAGCTGATGGTTGTA+TGG | + | chr8.3:28246917-28246936 | MS.gene89677:intron | 40.0% | |
| CATGCTGAAAATGTACCAGA+AGG | - | chr8.3:28246126-28246145 | None:intergenic | 40.0% | |
| CCAGTTCATAGCAGGATTTA+AGG | - | chr8.3:28246621-28246640 | None:intergenic | 40.0% | |
| CCTAATTACACATACCGACA+AGG | - | chr8.3:28247080-28247099 | None:intergenic | 40.0% | |
| CCTTAAATCCTGCTATGAAC+TGG | + | chr8.3:28246618-28246637 | MS.gene89677:intron | 40.0% | |
| CCTTGTCGGTATGTGTAATT+AGG | + | chr8.3:28247077-28247096 | MS.gene89677:intron | 40.0% | |
| GTAATATACTCCACAGTGCA+TGG | - | chr8.3:28245979-28245998 | None:intergenic | 40.0% | |
| TATATTACCCGGTACATAGC+TGG | + | chr8.3:28245991-28246010 | MS.gene89677:CDS | 40.0% | |
| TGAAGTTGCTGTGATGACTA+AGG | + | chr8.3:28247125-28247144 | MS.gene89677:CDS | 40.0% | |
| TGCAAAGAAAGCTCCTCTTA+AGG | + | chr8.3:28247230-28247249 | MS.gene89677:CDS | 40.0% | |
| TTACCTATGAAGCACAGACA+CGG | - | chr8.3:28245290-28245309 | None:intergenic | 40.0% | |
| ! | CAAAAGTACACACAAAGTGG+TGG | + | chr8.3:28246021-28246040 | MS.gene89677:CDS | 40.0% |
| ! | CACTTTGTGTGTACTTTTGC+TGG | - | chr8.3:28246020-28246039 | None:intergenic | 40.0% |
| ! | CAGCAAAAGTACACACAAAG+TGG | + | chr8.3:28246018-28246037 | MS.gene89677:CDS | 40.0% |
| ! | CTTCTGGTACATTTTCAGCA+TGG | + | chr8.3:28246124-28246143 | MS.gene89677:CDS | 40.0% |
| ! | GGTGTTAGGTTTAGGATCTT+AGG | + | chr8.3:28244925-28244944 | MS.gene89677:intron | 40.0% |
| ! | TAATTTGCATCGGAGTGGAT+TGG | + | chr8.3:28245069-28245088 | MS.gene89677:intron | 40.0% |
| ! | TGAAGTAATTTGCATCGGAG+TGG | + | chr8.3:28245064-28245083 | MS.gene89677:intron | 40.0% |
| ! | TGGTACATTTTCAGCATGGA+AGG | + | chr8.3:28246128-28246147 | MS.gene89677:CDS | 40.0% |
| !! | TTTTAAAGAGACCTCTGGAC+AGG | + | chr8.3:28246203-28246222 | MS.gene89677:CDS | 40.0% |
| AAGCCATACAACCATCAGCT+AGG | - | chr8.3:28246923-28246942 | None:intergenic | 45.0% | |
| ACATTGCTCTTGCTTGTGCT+GGG | + | chr8.3:28245878-28245897 | MS.gene89677:CDS | 45.0% | |
| ACGCCTTCAATCGTAAGTGT+CGG | - | chr8.3:28245200-28245219 | None:intergenic | 45.0% | |
| ACTACCTAGGAGTGGTACTT+TGG | + | chr8.3:28246402-28246421 | MS.gene89677:intron | 45.0% | |
| ACTTGGAAGAGATGACCATC+GGG | - | chr8.3:28244733-28244752 | None:intergenic | 45.0% | |
| AGAATCTTGAAGCTTAGCCG+TGG | - | chr8.3:28244845-28244864 | None:intergenic | 45.0% | |
| AGGATCAAAGCCAACGATCA+AGG | - | chr8.3:28246070-28246089 | None:intergenic | 45.0% | |
| ATCAAAGCCAACGATCAAGG+TGG | - | chr8.3:28246067-28246086 | None:intergenic | 45.0% | |
| ATCAAGGTGGAAAGCCCAAA+TGG | - | chr8.3:28246054-28246073 | None:intergenic | 45.0% | |
| CCAACAATTTACCCACACTG+TGG | + | chr8.3:28246583-28246602 | MS.gene89677:intron | 45.0% | |
| CCACAGTGTGGGTAAATTGT+TGG | - | chr8.3:28246586-28246605 | None:intergenic | 45.0% | |
| CGGCTAAGCTTCAAGATTCT+AGG | + | chr8.3:28244845-28244864 | MS.gene89677:CDS | 45.0% | |
| GACTTGGAAGAGATGACCAT+CGG | - | chr8.3:28244734-28244753 | None:intergenic | 45.0% | |
| GCACTGTGGAGTATATTACC+CGG | + | chr8.3:28245980-28245999 | MS.gene89677:CDS | 45.0% | |
| GTCTGCATTGTATAGCTAGC+AGG | - | chr8.3:28246762-28246781 | None:intergenic | 45.0% | |
| TGCTCGTGTCTTGATAAACC+GGG | + | chr8.3:28245912-28245931 | MS.gene89677:CDS | 45.0% | |
| TGCTTCAATCTCAGCCACAA+TGG | - | chr8.3:28247190-28247209 | None:intergenic | 45.0% | |
| TGGACAGGAAACTGTGAAGT+TGG | + | chr8.3:28246218-28246237 | MS.gene89677:CDS | 45.0% | |
| TGTAATTAGGTCGTCGAGAG+TGG | + | chr8.3:28247090-28247109 | MS.gene89677:intron | 45.0% | |
| ! | TCTTCCAAAGTACCACTCCT+AGG | - | chr8.3:28246409-28246428 | None:intergenic | 45.0% |
| ! | TGATGTTGACTACCTAGGAG+TGG | + | chr8.3:28246394-28246413 | MS.gene89677:intron | 45.0% |
| !! | AGTACGCTTTAGAGGCTGTT+AGG | + | chr8.3:28244755-28244774 | MS.gene89677:CDS | 45.0% |
| !! | GCTTTAGAGGCTGTTAGGAA+GGG | + | chr8.3:28244760-28244779 | MS.gene89677:CDS | 45.0% |
| !! | GGTATCGATAACGTCGTTCT+CGG | + | chr8.3:28244805-28244824 | MS.gene89677:CDS | 45.0% |
| !! | GTTGGCTTTGATCCTTACAC+CGG | + | chr8.3:28246075-28246094 | MS.gene89677:CDS | 45.0% |
| !! | TTGGCTTTGATCCTTACACC+GGG | + | chr8.3:28246076-28246095 | MS.gene89677:CDS | 45.0% |
| AATGGTCAGAGACCACAGTG+TGG | - | chr8.3:28246598-28246617 | None:intergenic | 50.0% | |
| AATTAGGTCGTCGAGAGTGG+TGG | + | chr8.3:28247093-28247112 | MS.gene89677:intron | 50.0% | |
| ATGGTCAGAGACCACAGTGT+GGG | - | chr8.3:28246597-28246616 | None:intergenic | 50.0% | |
| ATGTACCAGAAGGGTCTGTC+TGG | - | chr8.3:28246116-28246135 | None:intergenic | 50.0% | |
| CACAGTGCATGGATCTTCGA+CGG | - | chr8.3:28245968-28245987 | None:intergenic | 50.0% | |
| CACATTGCTCTTGCTTGTGC+TGG | + | chr8.3:28245877-28245896 | MS.gene89677:CDS | 50.0% | |
| CCAAGTCGAGTACGCTTTAG+AGG | + | chr8.3:28244747-28244766 | MS.gene89677:CDS | 50.0% | |
| CTTCACAGTTTCCTGTCCAG+AGG | - | chr8.3:28246217-28246236 | None:intergenic | 50.0% | |
| CTTGGAAGAGATGACCATCG+GGG | - | chr8.3:28244732-28244751 | None:intergenic | 50.0% | |
| GCTGTGATGACTAAGGAGCA+CGG | + | chr8.3:28247132-28247151 | MS.gene89677:CDS | 50.0% | |
| TCTGCTAAACAGGACCTACC+AGG | - | chr8.3:28245408-28245427 | None:intergenic | 50.0% | |
| TGGTAGGTCCTGTTTAGCAG+AGG | + | chr8.3:28245407-28245426 | MS.gene89677:intron | 50.0% | |
| TGTCCGTGTCTGTGCTTCAT+AGG | + | chr8.3:28245284-28245303 | MS.gene89677:intron | 50.0% | |
| TTGGAAGAGATGACCATCGG+GGG | - | chr8.3:28244731-28244750 | None:intergenic | 50.0% | |
| ! | ACACACAAAGTGGTGGTGTG+CGG | + | chr8.3:28246028-28246047 | MS.gene89677:CDS | 50.0% |
| ! | ATCACAGTTTTCTCCCCCGA+TGG | + | chr8.3:28244715-28244734 | MS.gene89677:CDS | 50.0% |
| ! | CGAATTCGAGTTTCTGCCAG+TGG | - | chr8.3:28246160-28246179 | None:intergenic | 50.0% |
| ! | CGGCGTTGAGAAGAAATCCA+CGG | + | chr8.3:28244825-28244844 | MS.gene89677:CDS | 50.0% |
| ! | GCACCGACACTTACGATTGA+AGG | + | chr8.3:28245194-28245213 | MS.gene89677:intron | 50.0% |
| !! | CCTCTAAAGCGTACTCGACT+TGG | - | chr8.3:28244750-28244769 | None:intergenic | 50.0% |
| !! | CGCTTTAGAGGCTGTTAGGA+AGG | + | chr8.3:28244759-28244778 | MS.gene89677:CDS | 50.0% |
| AGCAGAGAAAGCAGCTGCTG+AGG | + | chr8.3:28247206-28247225 | MS.gene89677:CDS | 55.0% | |
| AGGGTCTGTCTGGTACAGAG+AGG | - | chr8.3:28246106-28246125 | None:intergenic | 55.0% | |
| ATCGATACCGCGAACACCGA+CGG | - | chr8.3:28244794-28244813 | None:intergenic | 55.0% | |
| CGTCGAAGATCCATGCACTG+TGG | + | chr8.3:28245966-28245985 | MS.gene89677:CDS | 55.0% | |
| CTGGAGACCAGCTATGTACC+GGG | - | chr8.3:28246001-28246020 | None:intergenic | 55.0% | |
| CTGTACCAGACAGACCCTTC+TGG | + | chr8.3:28246108-28246127 | MS.gene89677:CDS | 55.0% | |
| GCATGGAAGGCAAATGCCAC+TGG | + | chr8.3:28246141-28246160 | MS.gene89677:CDS | 55.0% | |
| GCGTTGAATGCCAGAGTCAC+AGG | + | chr8.3:28245938-28245957 | MS.gene89677:CDS | 55.0% | |
| GCTGGAGACCAGCTATGTAC+CGG | - | chr8.3:28246002-28246021 | None:intergenic | 55.0% | |
| GGGCTTTCCACCTTGATCGT+TGG | + | chr8.3:28246057-28246076 | MS.gene89677:CDS | 55.0% | |
| GGGTCTGTCTGGTACAGAGA+GGG | - | chr8.3:28246105-28246124 | None:intergenic | 55.0% | |
| AGAGAGGGAGACCCGGTGTA+AGG | - | chr8.3:28246090-28246109 | None:intergenic | 60.0% | |
| CTGGTACAGAGAGGGAGACC+CGG | - | chr8.3:28246097-28246116 | None:intergenic | 60.0% | |
| GCACGGTCTGCGTCAATTGG+AGG | + | chr8.3:28247149-28247168 | MS.gene89677:CDS | 60.0% | |
| GGAGCACGGTCTGCGTCAAT+TGG | + | chr8.3:28247146-28247165 | MS.gene89677:CDS | 60.0% | |
| TCTGGCATTCAACGCGTGCC+CGG | - | chr8.3:28245933-28245952 | None:intergenic | 60.0% | |
| ! | AGTGGTGGTGTGCGGCCATT+TGG | + | chr8.3:28246036-28246055 | MS.gene89677:CDS | 60.0% |
| ! | GTGGTGGTGTGCGGCCATTT+GGG | + | chr8.3:28246037-28246056 | MS.gene89677:CDS | 60.0% |
| AGGAAGGGTAACGCCGCCGT+CGG | + | chr8.3:28244775-28244794 | MS.gene89677:CDS | 65.0% | |
| CGACGGTGAGCCTGTGACTC+TGG | - | chr8.3:28245951-28245970 | None:intergenic | 65.0% | |
| GATACCGCGAACACCGACGG+CGG | - | chr8.3:28244791-28244810 | None:intergenic | 65.0% | |
| ! | AACGCCGCCGTCGGTGTTCG+CGG | + | chr8.3:28244784-28244803 | MS.gene89677:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 28244694 | 28247260 | 28244694 | ID=MS.gene89677 |
| chr8.3 | mRNA | 28244694 | 28247260 | 28244694 | ID=MS.gene89677.t1;Parent=MS.gene89677 |
| chr8.3 | exon | 28244694 | 28244866 | 28244694 | ID=MS.gene89677.t1.exon1;Parent=MS.gene89677.t1 |
| chr8.3 | CDS | 28244694 | 28244866 | 28244694 | ID=cds.MS.gene89677.t1;Parent=MS.gene89677.t1 |
| chr8.3 | exon | 28245846 | 28246260 | 28245846 | ID=MS.gene89677.t1.exon2;Parent=MS.gene89677.t1 |
| chr8.3 | CDS | 28245846 | 28246260 | 28245846 | ID=cds.MS.gene89677.t1;Parent=MS.gene89677.t1 |
| chr8.3 | exon | 28247099 | 28247260 | 28247099 | ID=MS.gene89677.t1.exon3;Parent=MS.gene89677.t1 |
| chr8.3 | CDS | 28247099 | 28247260 | 28247099 | ID=cds.MS.gene89677.t1;Parent=MS.gene89677.t1 |
| Gene Sequence |
| Protein sequence |
