AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44141


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44141.t1 MTR_1g083460 97.080 137 4 0 1 137 1 137 2.71e-93 268
MS.gene44141.t1 MTR_7g108805 91.971 137 11 0 1 137 1 137 2.47e-88 256
MS.gene44141.t1 MTR_7g112880 82.482 137 24 0 1 137 1 137 7.29e-81 237
MS.gene44141.t1 MTR_7g112880 78.049 82 18 0 54 135 1 82 5.33e-44 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44141.t1 AT5G27850 82.482 137 24 0 1 137 1 137 6.47e-80 234
MS.gene44141.t1 AT3G05590 81.752 137 25 0 1 137 1 137 1.14e-78 231
MS.gene44141.t1 AT2G47570 74.265 136 34 1 3 137 1 136 2.33e-68 205
MS.gene44141.t1 AT3G05590 81.707 82 15 0 54 135 1 82 1.15e-44 143
MS.gene44141.t1 AT3G05590 81.707 82 15 0 54 135 1 82 1.15e-44 143
MS.gene44141.t1 AT5G27850 80.488 82 16 0 54 135 1 82 5.59e-44 142

Find 44 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTATTGCTGTCATTTAA+TGG 0.159518 1.3:+59056590 MS.gene44141:CDS
CATTCGATCAGTTGGCTCTT+AGG 0.165548 1.3:+59056366 MS.gene44141:CDS
TATTCTTGCCCTTGCCTTCT+CGG 0.231639 1.3:-59056318 None:intergenic
AGATAGATATCATTGGATTT+TGG 0.265066 1.3:-59055210 None:intergenic
CACTATAGTGATTCCTTTGT+TGG 0.281093 1.3:-59056570 None:intergenic
TGATATCTATCTCAAGCTTT+TGG 0.326212 1.3:+59055221 MS.gene44141:CDS
GTTGGCTCTTAGGGCCCCTC+TGG 0.332505 1.3:+59056376 MS.gene44141:CDS
AGCTTTATCGGTTCCTTGTT+CGG 0.346996 1.3:+59055997 MS.gene44141:intron
CGGTTCCTTGTTCGGAGAAC+TGG 0.351555 1.3:+59056005 MS.gene44141:CDS
ATTCGATCAGTTGGCTCTTA+GGG 0.374062 1.3:+59056367 MS.gene44141:CDS
CCTGAGAGCTGTAACCTTGA+TGG 0.403591 1.3:-59056291 None:intergenic
GGTAAAATTGCTGTCGTGGT+GGG 0.429219 1.3:+59056230 MS.gene44141:CDS
CCATCAAGGTTACAGCTCTC+AGG 0.444373 1.3:+59056291 MS.gene44141:CDS
AAGCTTGAGATAGATATCAT+TGG 0.447582 1.3:-59055217 None:intergenic
TTTGTAGGGTATCGATCTTA+AGG 0.453950 1.3:+59055152 MS.gene44141:intron
ATCAACCTTGACAAAGATAG+TGG 0.491899 1.3:-59056086 None:intergenic
GTAAAATTGCTGTCGTGGTG+GGG 0.493129 1.3:+59056231 MS.gene44141:CDS
CAAGAGGTTGTTTATGAGCA+AGG 0.493391 1.3:+59056052 MS.gene44141:CDS
ATGCTTAACATTCGATCAGT+TGG 0.511340 1.3:+59056358 MS.gene44141:CDS
TAATGAAGTTCCAGCCATCA+AGG 0.511887 1.3:+59056277 MS.gene44141:CDS
TTCTCTCTAGTCTCCAACAA+AGG 0.526953 1.3:+59056557 MS.gene44141:CDS
AGCTCTCAGGTTTACCGAGA+AGG 0.539540 1.3:+59056304 MS.gene44141:CDS
TTGGCTCTTAGGGCCCCTCT+GGG 0.540521 1.3:+59056377 MS.gene44141:CDS
AGGTAAAATTGCTGTCGTGG+TGG 0.541180 1.3:+59056229 MS.gene44141:CDS
TAAAATTGCTGTCGTGGTGG+GGG 0.545281 1.3:+59056232 MS.gene44141:CDS
TCAATGCTGTTATACTCAAG+AGG 0.549427 1.3:+59056036 MS.gene44141:CDS
TCAGGTTTACCGAGAAGGCA+AGG 0.549678 1.3:+59056309 MS.gene44141:CDS
TGCTGCCAGTTCTCCGAACA+AGG 0.549972 1.3:-59056010 None:intergenic
AGGCAAGGGCAAGAATAGAA+AGG 0.551093 1.3:+59056324 MS.gene44141:CDS
GGCAAGGGCAAGAATAGAAA+GGG 0.553936 1.3:+59056325 MS.gene44141:CDS
AGATTGACAAACCTTGCCAT+TGG 0.555253 1.3:-59056117 None:intergenic
TAGGGTATCGATCTTAAGGC+AGG 0.555670 1.3:+59055156 MS.gene44141:intron
AGAGCTGTAACCTTGATGGC+TGG 0.558387 1.3:-59056287 None:intergenic
AACCTCCACTATCTTTGTCA+AGG 0.576324 1.3:+59056081 MS.gene44141:CDS
GGCCCCTCTGGGACAAAACA+CGG 0.583895 1.3:+59056388 MS.gene44141:CDS
CAGGTTTACCGAGAAGGCAA+GGG 0.596428 1.3:+59056310 MS.gene44141:CDS
AGGGCAAGAATAGAAAGGGC+TGG 0.616183 1.3:+59056329 MS.gene44141:CDS
GATCAAGTATACCAATGGCA+AGG 0.626076 1.3:+59056106 MS.gene44141:CDS
TGGCTGGAACTTCATTAACA+CGG 0.640444 1.3:-59056271 None:intergenic
AGGTTGATCAAGTATACCAA+TGG 0.643281 1.3:+59056101 MS.gene44141:CDS
AACCTTGACAAAGATAGTGG+AGG 0.643571 1.3:-59056083 None:intergenic
GGAAGGTAAAATTGCTGTCG+TGG 0.652028 1.3:+59056226 MS.gene44141:intron
GGTATCGATCTTAAGGCAGG+AGG 0.692243 1.3:+59055159 MS.gene44141:CDS
GCAAGAATAGAAAGGGCTGG+TGG 0.708893 1.3:+59056332 MS.gene44141:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAATAATAATTT+AGG - chr1.3:59055673-59055692 None:intergenic 0.0%
!!! TATTATTGTTTTAAAAAACA+AGG + chr1.3:59055686-59055705 MS.gene44141:intron 10.0%
!!! TGTTTTCAAAATATTATTAT+GGG + chr1.3:59055589-59055608 MS.gene44141:intron 10.0%
!! AATGAAATATAACCAAAAAT+AGG - chr1.3:59055539-59055558 None:intergenic 15.0%
!!! CTGTTTTCAAAATATTATTA+TGG + chr1.3:59055588-59055607 MS.gene44141:intron 15.0%
!!! GTTTTAGCTTTATAAAAAAA+TGG - chr1.3:59055857-59055876 None:intergenic 15.0%
!!! TTAATAGAAGTGATTTTTAT+GGG + chr1.3:59055752-59055771 MS.gene44141:intron 15.0%
!! CATAGTCAAAAAAAATCATT+GGG - chr1.3:59055375-59055394 None:intergenic 20.0%
!! TAGATATATGAGAAAAATGA+GGG - chr1.3:59055887-59055906 None:intergenic 20.0%
!! TCAGTTTACTATAGTTATAA+TGG + chr1.3:59056177-59056196 MS.gene44141:intron 20.0%
!!! AAGTTTAATTTATAATGCTG+AGG + chr1.3:59055420-59055439 MS.gene44141:intron 20.0%
!!! AGTTTAATTTATAATGCTGA+GGG + chr1.3:59055421-59055440 MS.gene44141:intron 20.0%
!!! GTTAATAGAAGTGATTTTTA+TGG + chr1.3:59055751-59055770 MS.gene44141:intron 20.0%
!!! TATTGTTTTAAAAAACAAGG+AGG + chr1.3:59055689-59055708 MS.gene44141:intron 20.0%
!!! TTACATTGAATTGTTTTGAT+TGG + chr1.3:59055961-59055980 MS.gene44141:intron 20.0%
!!! TTTTTGGTTATATTTCATTG+CGG + chr1.3:59055540-59055559 MS.gene44141:intron 20.0%
! AAGAAATAACATAAAGCTAC+AGG - chr1.3:59055305-59055324 None:intergenic 25.0%
! AATTCAAACGTAAGAAACAA+TGG - chr1.3:59055128-59055147 None:intergenic 25.0%
! AGAAATAACATAAAGCTACA+GGG - chr1.3:59055304-59055323 None:intergenic 25.0%
! CAAAAAGACGTTAAATAATC+AGG - chr1.3:59056510-59056529 None:intergenic 25.0%
! GCATAGTCAAAAAAAATCAT+TGG - chr1.3:59055376-59055395 None:intergenic 25.0%
! GTAGATATATGAGAAAAATG+AGG - chr1.3:59055888-59055907 None:intergenic 25.0%
! GTTGAAATGTAAGCAAAAAT+GGG + chr1.3:59055504-59055523 MS.gene44141:intron 25.0%
! TGCATAGGTAAATTTCAAAA+TGG - chr1.3:59056206-59056225 None:intergenic 25.0%
! TGTTGAAATGTAAGCAAAAA+TGG + chr1.3:59055503-59055522 MS.gene44141:intron 25.0%
!! AGATAGATATCATTGGATTT+TGG - chr1.3:59055213-59055232 None:intergenic 25.0%
!! TGTCATTGACAAACAATTTT+AGG + chr1.3:59055329-59055348 MS.gene44141:intron 25.0%
!!! ATTTTGAAATTTACCTATGC+AGG + chr1.3:59056205-59056224 MS.gene44141:intron 25.0%
!!! TGATTGAACTTTTTAGAGTA+TGG + chr1.3:59056528-59056547 MS.gene44141:intron 25.0%
!!! TTTTACATGATCAGCTTTAT+CGG + chr1.3:59055985-59056004 MS.gene44141:intron 25.0%
AAGCTTGAGATAGATATCAT+TGG - chr1.3:59055220-59055239 None:intergenic 30.0%
ACGTTTGAATTGAACTTTGT+AGG + chr1.3:59055137-59055156 MS.gene44141:intron 30.0%
CGTTTGAATTGAACTTTGTA+GGG + chr1.3:59055138-59055157 MS.gene44141:intron 30.0%
TATTATGGGTGTGTTTGTTT+AGG + chr1.3:59055603-59055622 MS.gene44141:intron 30.0%
TTGCTTACATTTCAACATAG+AGG - chr1.3:59055500-59055519 None:intergenic 30.0%
! AGGAAAAGCTAACTCATTTT+AGG - chr1.3:59055653-59055672 None:intergenic 30.0%
! GGAAAAGCTAACTCATTTTA+GGG - chr1.3:59055652-59055671 None:intergenic 30.0%
! TGATATCTATCTCAAGCTTT+TGG + chr1.3:59055221-59055240 MS.gene44141:CDS 30.0%
ATCAACCTTGACAAAGATAG+TGG - chr1.3:59056089-59056108 None:intergenic 35.0%
ATGCTTAACATTCGATCAGT+TGG + chr1.3:59056358-59056377 MS.gene44141:CDS 35.0%
CACTATAGTGATTCCTTTGT+TGG - chr1.3:59056573-59056592 None:intergenic 35.0%
TCAATGCTGTTATACTCAAG+AGG + chr1.3:59056036-59056055 MS.gene44141:CDS 35.0%
TGAAATTTACCTATGCAGGA+AGG + chr1.3:59056209-59056228 MS.gene44141:intron 35.0%
TGGTTATATTTCATTGCGGA+TGG + chr1.3:59055544-59055563 MS.gene44141:intron 35.0%
! AGGTTGATCAAGTATACCAA+TGG + chr1.3:59056101-59056120 MS.gene44141:CDS 35.0%
! GGGTCGAAAAAACCTATTTT+TGG + chr1.3:59055524-59055543 MS.gene44141:intron 35.0%
!! TTTGTAGGGTATCGATCTTA+AGG + chr1.3:59055152-59055171 MS.gene44141:intron 35.0%
AACCTCCACTATCTTTGTCA+AGG + chr1.3:59056081-59056100 MS.gene44141:CDS 40.0%
AGATTGACAAACCTTGCCAT+TGG - chr1.3:59056120-59056139 None:intergenic 40.0%
CAAGAGGTTGTTTATGAGCA+AGG + chr1.3:59056052-59056071 MS.gene44141:CDS 40.0%
GATCAAGTATACCAATGGCA+AGG + chr1.3:59056106-59056125 MS.gene44141:CDS 40.0%
TAATGAAGTTCCAGCCATCA+AGG + chr1.3:59056277-59056296 MS.gene44141:CDS 40.0%
TGGCTGGAACTTCATTAACA+CGG - chr1.3:59056274-59056293 None:intergenic 40.0%
TTCTCTCTAGTCTCCAACAA+AGG + chr1.3:59056557-59056576 MS.gene44141:CDS 40.0%
! AAACACGGTATGTGTCATTC+TGG + chr1.3:59056403-59056422 MS.gene44141:intron 40.0%
! AACCTTGACAAAGATAGTGG+AGG - chr1.3:59056086-59056105 None:intergenic 40.0%
! ATTCGATCAGTTGGCTCTTA+GGG + chr1.3:59056367-59056386 MS.gene44141:CDS 40.0%
! CTATCTCAAGCTTTTGGTCA+AGG + chr1.3:59055227-59055246 MS.gene44141:CDS 40.0%
! GCAATTTTACCTTCCTGCAT+AGG - chr1.3:59056221-59056240 None:intergenic 40.0%
!! AGCTTTATCGGTTCCTTGTT+CGG + chr1.3:59055997-59056016 MS.gene44141:intron 40.0%
AGGCAAGGGCAAGAATAGAA+AGG + chr1.3:59056324-59056343 MS.gene44141:CDS 45.0%
AGGTAAAATTGCTGTCGTGG+TGG + chr1.3:59056229-59056248 MS.gene44141:CDS 45.0%
GGAAGGTAAAATTGCTGTCG+TGG + chr1.3:59056226-59056245 MS.gene44141:intron 45.0%
GGCAAGGGCAAGAATAGAAA+GGG + chr1.3:59056325-59056344 MS.gene44141:CDS 45.0%
GGTAAAATTGCTGTCGTGGT+GGG + chr1.3:59056230-59056249 MS.gene44141:CDS 45.0%
GTAAAATTGCTGTCGTGGTG+GGG + chr1.3:59056231-59056250 MS.gene44141:CDS 45.0%
TAAAATTGCTGTCGTGGTGG+GGG + chr1.3:59056232-59056251 MS.gene44141:CDS 45.0%
TATTCTTGCCCTTGCCTTCT+CGG - chr1.3:59056321-59056340 None:intergenic 45.0%
! ATACCGTGTTTTGTCCCAGA+GGG - chr1.3:59056394-59056413 None:intergenic 45.0%
! CATTCGATCAGTTGGCTCTT+AGG + chr1.3:59056366-59056385 MS.gene44141:CDS 45.0%
!! TAGGGTATCGATCTTAAGGC+AGG + chr1.3:59055156-59055175 MS.gene44141:intron 45.0%
AGCTCTCAGGTTTACCGAGA+AGG + chr1.3:59056304-59056323 MS.gene44141:CDS 50.0%
AGGGCAAGAATAGAAAGGGC+TGG + chr1.3:59056329-59056348 MS.gene44141:CDS 50.0%
CAGGTTTACCGAGAAGGCAA+GGG + chr1.3:59056310-59056329 MS.gene44141:CDS 50.0%
CCATCAAGGTTACAGCTCTC+AGG + chr1.3:59056291-59056310 MS.gene44141:CDS 50.0%
CCTGAGAGCTGTAACCTTGA+TGG - chr1.3:59056294-59056313 None:intergenic 50.0%
GCAAGAATAGAAAGGGCTGG+TGG + chr1.3:59056332-59056351 MS.gene44141:CDS 50.0%
TCAGGTTTACCGAGAAGGCA+AGG + chr1.3:59056309-59056328 MS.gene44141:CDS 50.0%
! AGAGCTGTAACCTTGATGGC+TGG - chr1.3:59056290-59056309 None:intergenic 50.0%
! CATACCGTGTTTTGTCCCAG+AGG - chr1.3:59056395-59056414 None:intergenic 50.0%
! TACCGTGTTTTGTCCCAGAG+GGG - chr1.3:59056393-59056412 None:intergenic 50.0%
!! GGTATCGATCTTAAGGCAGG+AGG + chr1.3:59055159-59055178 MS.gene44141:CDS 50.0%
CGGTTCCTTGTTCGGAGAAC+TGG + chr1.3:59056005-59056024 MS.gene44141:CDS 55.0%
TGCTGCCAGTTCTCCGAACA+AGG - chr1.3:59056013-59056032 None:intergenic 55.0%
! TTGGCTCTTAGGGCCCCTCT+GGG + chr1.3:59056377-59056396 MS.gene44141:CDS 60.0%
!! GGCCCCTCTGGGACAAAACA+CGG + chr1.3:59056388-59056407 MS.gene44141:CDS 60.0%
! GTTGGCTCTTAGGGCCCCTC+TGG + chr1.3:59056376-59056395 MS.gene44141:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 59055065 59056609 59055065 ID=MS.gene44141
chr1.3 mRNA 59055065 59056609 59055065 ID=MS.gene44141.t1;Parent=MS.gene44141
chr1.3 exon 59055065 59055067 59055065 ID=MS.gene44141.t1.exon1;Parent=MS.gene44141.t1
chr1.3 CDS 59055065 59055067 59055065 ID=cds.MS.gene44141.t1;Parent=MS.gene44141.t1
chr1.3 exon 59055159 59055248 59055159 ID=MS.gene44141.t1.exon2;Parent=MS.gene44141.t1
chr1.3 CDS 59055159 59055248 59055159 ID=cds.MS.gene44141.t1;Parent=MS.gene44141.t1
chr1.3 exon 59055999 59056127 59055999 ID=MS.gene44141.t1.exon3;Parent=MS.gene44141.t1
chr1.3 CDS 59055999 59056127 59055999 ID=cds.MS.gene44141.t1;Parent=MS.gene44141.t1
chr1.3 exon 59056227 59056409 59056227 ID=MS.gene44141.t1.exon4;Parent=MS.gene44141.t1
chr1.3 CDS 59056227 59056409 59056227 ID=cds.MS.gene44141.t1;Parent=MS.gene44141.t1
chr1.3 exon 59056544 59056609 59056544 ID=MS.gene44141.t1.exon5;Parent=MS.gene44141.t1
chr1.3 CDS 59056544 59056609 59056544 ID=cds.MS.gene44141.t1;Parent=MS.gene44141.t1
Gene Sequence

>MS.gene44141

ATGGTTTGTCTTCTCTTAAACAATCTTGTTATCATTCTGTTATGTATTTGAAGCGAAGCTCCATTGTTTCTTACGTTTGAATTGAACTTTGTAGGGTATCGATCTTAAGGCAGGAGGTAAGAACAAAAAAACAAAAAGAACAGCACCAAAATCCAATGATATCTATCTCAAGCTTTTGGTCAAGGTCAGTTTTATGTTTCTTTAGATCTGAATGAATTTTAACATTGTTGTGTTGACCCTGTAGCTTTATGTTATTTCTTGATCTGTCATTGACAAACAATTTTAGGCTTGTAACAATGAGAAAGTTCCCAATGATTTTTTTTGACTATGCTAATGCTGATAATTGATGATTAATAAGTTTAATTTATAATGCTGAGGGAATTAGTTAACTCTTTAATTCTGTTTTGTTATCAATGTTGAAGCTTACTACTTCCTCTATGTTGAAATGTAAGCAAAAATGGGTCGAAAAAACCTATTTTTGGTTATATTTCATTGCGGATGGATCACTTCATCTCAGAAGAATCTGTTTTCAAAATATTATTATGGGTGTGTTTGTTTAGGATTTAAAAAAAAATCATTTTTGACCCTAAAATGAGTTAGCTTTTCCTAAATTATTATTATTATTATTGTTTTAAAAAACAAGGAGGTAAATCATAAAAACATTAAAAATTATAAATCATGAACAAGTTAATAGAAGTGATTTTTATGGGCAGTAAAAAACAGAAATTGTTCAGATTTTTTTTTTTTTAAATCTCTAAAAATTAATCTCTTGATATTTTATCACATAATCCATTTTTTTATAAAGCTAAAACAAACGCACCCTCATTTTTCTCATATATCTACATGCATATTCATTTGAGATTTATGTCTTTTTTTTTGTAATATCTTTTGAATATTTACATTGAATTGTTTTGATTGGATTTTACATGATCAGCTTTATCGGTTCCTTGTTCGGAGAACTGGCAGCAATTTCAATGCTGTTATACTCAAGAGGTTGTTTATGAGCAAGGTTAACAAACCTCCACTATCTTTGTCAAGGTTGATCAAGTATACCAATGGCAAGGTTTGTCAATCTATGCAATCTTCATTTTCATAGTATTTTTTTTTGTAAATCAGTTTACTATAGTTATAATGGTTACCATTTTGAAATTTACCTATGCAGGAAGGTAAAATTGCTGTCGTGGTGGGGGCTATAACTGATGATGTCCGTGTTAATGAAGTTCCAGCCATCAAGGTTACAGCTCTCAGGTTTACCGAGAAGGCAAGGGCAAGAATAGAAAGGGCTGGTGGTGAATGCTTAACATTCGATCAGTTGGCTCTTAGGGCCCCTCTGGGACAAAACACGGTATGTGTCATTCTGGCATTAAGCTTTTGTCAGCTTCTGAGTAATTGACTCTTATCAGTTGCTGCATTTTTTGTGAAATGATTGAATTAGTTCTGCTCCTGATTATTTAACGTCTTTTTGATTGAACTTTTTAGAGTATGGAATTTGTTCTCTCTAGTCTCCAACAAAGGAATCACTATAGTGCTATTGCTGTCATTTAA

Protein sequence

>MS.gene44141.t1

MGIDLKAGGKNKKTKRTAPKSNDIYLKLLVKLYRFLVRRTGSNFNAVILKRLFMSKVNKPPLSLSRLIKYTNGKEGKIAVVVGAITDDVRVNEVPAIKVTALRFTEKARARIERAGGECLTFDQLALRAPLGQNTSMEFVLSSLQQRNHYSAIAVI