AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069310.t1 MTR_3g013635 99.167 120 1 0 1 120 1 120 1.65e-88 253
MS.gene069310.t1 MTR_3g013635 99.167 120 1 0 1 120 1 120 1.65e-88 253
MS.gene069310.t1 MTR_8g040740 88.333 120 14 0 1 120 1 120 4.11e-81 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069310.t1 AT5G61170 82.500 120 21 0 1 120 1 120 2.79e-77 224
MS.gene069310.t1 AT3G02080 81.667 120 22 0 1 120 1 120 1.26e-76 223
MS.gene069310.t1 AT5G15520 82.500 120 21 0 1 120 1 120 2.56e-76 222

Find 40 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAAGACTGCAAGGTTTA+AGG 0.181111 3.3:+8524198 MS.gene069310:CDS
GGTGCATTCCAGAGGATTTA+TGG 0.279212 3.3:+8524436 MS.gene069310:CDS
TTCACAATATCTGTCCATTC+TGG 0.341460 3.3:-8524181 None:intergenic
CCTTGGTGTCCATCTCAATA+AGG 0.385015 3.3:-8524552 None:intergenic
GACTGCAAGGTTTAAGGAGT+TGG 0.416477 3.3:+8524204 MS.gene069310:CDS
ACCCCTCAAGTAGATCTTCC+TGG 0.431786 3.3:-8524401 None:intergenic
GACTTCCACCATAAATCCTC+TGG 0.453242 3.3:-8524444 None:intergenic
ATCTACTTGAGGGGTGGACT+CGG 0.454423 3.3:+8524409 MS.gene069310:CDS
TCTGCTCATCTCAAAAGATC+TGG 0.481965 3.3:+8523262 MS.gene069310:CDS
GTGCCGCAGCTTCCATGGCC+AGG 0.485644 3.3:+8524383 MS.gene069310:intron
GCTCTGACGTAGTACCAGTC+AGG 0.489319 3.3:-8524238 None:intergenic
TAGTACCAGTCAGGGTCATA+AGG 0.498306 3.3:-8524229 None:intergenic
TATGGTGGAAGTCAGAGAAA+TGG 0.514768 3.3:+8524454 MS.gene069310:CDS
CAAGTAGATCTTCCTGGCCA+TGG 0.517027 3.3:-8524395 None:intergenic
GGCCAGGAAGATCTACTTGA+GGG 0.518439 3.3:+8524399 MS.gene069310:CDS
TTGAGGGGTGGACTCGGTGT+TGG 0.522669 3.3:+8524415 MS.gene069310:CDS
AACAGTCCTTGCGGTAGCCA+TGG 0.523894 3.3:-8523203 None:intergenic
GATAATGAGTTCTTACCCCT+TGG 0.524570 3.3:-8524569 None:intergenic
TGGCTCCTTATGACCCTGAC+TGG 0.535373 3.3:+8524224 MS.gene069310:CDS
CCACTGCTCTTGCAGAAATG+AGG 0.542089 3.3:-8524487 None:intergenic
GACTGGTACTACGTCAGAGC+TGG 0.543992 3.3:+8524241 MS.gene069310:CDS
AACAGATGGAGCTTCCAGAA+TGG 0.551656 3.3:+8524167 MS.gene069310:intron
TTGAAGAATGTGGCGAGCAA+TGG 0.555153 3.3:-8524512 None:intergenic
GAACATGAACCTTATTGAGA+TGG 0.561257 3.3:+8524543 MS.gene069310:CDS
CCTCATTTCTGCAAGAGCAG+TGG 0.589477 3.3:+8524487 MS.gene069310:CDS
TCGGTGTTGGTGCATTCCAG+AGG 0.603387 3.3:+8524428 MS.gene069310:CDS
CAGATATTGTGAAGACTGCA+AGG 0.617924 3.3:+8524191 MS.gene069310:CDS
CTGCTCTTGCAGAAATGAGG+TGG 0.621206 3.3:-8524484 None:intergenic
AGGAAGATCTACTTGAGGGG+TGG 0.621735 3.3:+8524403 MS.gene069310:CDS
CTCTGACGTAGTACCAGTCA+GGG 0.627550 3.3:-8524237 None:intergenic
TCATCTCAAAAGATCTGGCA+AGG 0.635281 3.3:+8523267 MS.gene069310:CDS
GCACAACCATGGCTACCGCA+AGG 0.635383 3.3:+8523197 None:intergenic
CTTATTGAGATGGACACCAA+GGG 0.649426 3.3:+8524553 MS.gene069310:CDS
TGGCCAGGAAGATCTACTTG+AGG 0.650322 3.3:+8524398 MS.gene069310:CDS
CCTTATTGAGATGGACACCA+AGG 0.671459 3.3:+8524552 MS.gene069310:CDS
GCATTCCAGAGGATTTATGG+TGG 0.673361 3.3:+8524439 MS.gene069310:CDS
AACATCTTGAACAGTCCTTG+CGG 0.675305 3.3:-8523212 None:intergenic
TCTGCAACTGTTGAAGAATG+TGG 0.679137 3.3:-8524522 None:intergenic
GCCAGGAAGATCTACTTGAG+GGG 0.686642 3.3:+8524400 MS.gene069310:CDS
TTATTGAGATGGACACCAAG+GGG 0.704884 3.3:+8524554 MS.gene069310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTAATTTAAACAA+GGG - chr3.3:8524303-8524322 None:intergenic 10.0%
!!! TTTTTTTGTTAATTTAAACA+AGG - chr3.3:8524304-8524323 None:intergenic 10.0%
!! TAATCTATGTAATTAGAATA+AGG - chr3.3:8523935-8523954 None:intergenic 15.0%
!!! AAAACCAAAATGAAATTTTT+CGG - chr3.3:8523305-8523324 None:intergenic 15.0%
!!! ATCAAAATCATTTTTTTGTT+AGG - chr3.3:8523610-8523629 None:intergenic 15.0%
!!! TTGTTTTTGTTAATCAAATT+AGG + chr3.3:8523811-8523830 MS.gene069310:intron 15.0%
!!! TTTGATTTTTTTTTTTGAAG+AGG + chr3.3:8523354-8523373 MS.gene069310:intron 15.0%
!! AACAAAAAAATAACTTTCGA+GGG + chr3.3:8524314-8524333 MS.gene069310:intron 20.0%
!! ATTTGAACTATACAATTACA+CGG - chr3.3:8524094-8524113 None:intergenic 20.0%
!! TAACAAAAAAATAACTTTCG+AGG + chr3.3:8524313-8524332 MS.gene069310:intron 20.0%
!! TCTAATTACATAGATTACTT+GGG + chr3.3:8523938-8523957 MS.gene069310:intron 20.0%
!! TTATAATGATACGAATGAAA+TGG + chr3.3:8524068-8524087 MS.gene069310:intron 20.0%
!! TTCTAATTACATAGATTACT+TGG + chr3.3:8523937-8523956 MS.gene069310:intron 20.0%
!! TTGTATAGTTCAAATTTAGT+TGG + chr3.3:8524099-8524118 MS.gene069310:intron 20.0%
!!! ACAAATTTTGAAAGTTCAAA+AGG - chr3.3:8523904-8523923 None:intergenic 20.0%
!!! ATTTGTTTTGATTTGCTTAA+CGG + chr3.3:8523378-8523397 MS.gene069310:intron 20.0%
!!! CAAATTTTGAAAGTTCAAAA+GGG - chr3.3:8523903-8523922 None:intergenic 20.0%
!!! TGATTTTGATTTGATTTGAT+TGG + chr3.3:8523620-8523639 MS.gene069310:intron 20.0%
!!! TGTTTTGAATGTTATGTTTT+TGG + chr3.3:8523509-8523528 MS.gene069310:intron 20.0%
! CATCTGTTCATAACAAAATA+AGG - chr3.3:8524155-8524174 None:intergenic 25.0%
!! TTCACCGAAAAATTTCATTT+TGG + chr3.3:8523298-8523317 MS.gene069310:intron 25.0%
!!! CTTATTTTGTTATGAACAGA+TGG + chr3.3:8524153-8524172 MS.gene069310:intron 25.0%
!!! GAACTTTCAAAATTTGTTGT+TGG + chr3.3:8523907-8523926 MS.gene069310:intron 25.0%
!!! TGAATGTTATGTTTTTGGTT+GGG + chr3.3:8523514-8523533 MS.gene069310:intron 25.0%
!!! TTGAATGTTATGTTTTTGGT+TGG + chr3.3:8523513-8523532 MS.gene069310:intron 25.0%
!!! TTTGTTTTGATTTGCTTAAC+GGG + chr3.3:8523379-8523398 MS.gene069310:intron 25.0%
! ACATTGCAATTTTCAACAAC+AGG - chr3.3:8524356-8524375 None:intergenic 30.0%
GAACATGAACCTTATTGAGA+TGG + chr3.3:8524543-8524562 MS.gene069310:CDS 35.0%
GTTAATTTAAACAAGGGACC+AGG - chr3.3:8524297-8524316 None:intergenic 35.0%
TTACATAGATTACTTGGGCA+TGG + chr3.3:8523943-8523962 MS.gene069310:intron 35.0%
TTCACAATATCTGTCCATTC+TGG - chr3.3:8524184-8524203 None:intergenic 35.0%
!! GTTTGATTTTGTGCATTTCC+TGG + chr3.3:8524276-8524295 MS.gene069310:intron 35.0%
!!! GTTATGTTTTTGGTTGGGTT+TGG + chr3.3:8523519-8523538 MS.gene069310:intron 35.0%
AACATCTTGAACAGTCCTTG+CGG - chr3.3:8523215-8523234 None:intergenic 40.0%
CAGATATTGTGAAGACTGCA+AGG + chr3.3:8524191-8524210 MS.gene069310:CDS 40.0%
CTTATTGAGATGGACACCAA+GGG + chr3.3:8524553-8524572 MS.gene069310:CDS 40.0%
GTATTCAAGCTCTACTTCAC+TGG + chr3.3:8524034-8524053 MS.gene069310:intron 40.0%
TCATCTCAAAAGATCTGGCA+AGG + chr3.3:8523267-8523286 MS.gene069310:CDS 40.0%
TCTGCAACTGTTGAAGAATG+TGG - chr3.3:8524525-8524544 None:intergenic 40.0%
TCTGCTCATCTCAAAAGATC+TGG + chr3.3:8523262-8523281 MS.gene069310:CDS 40.0%
TGTGAAGACTGCAAGGTTTA+AGG + chr3.3:8524198-8524217 MS.gene069310:CDS 40.0%
TTATTGAGATGGACACCAAG+GGG + chr3.3:8524554-8524573 MS.gene069310:CDS 40.0%
! TATGGTGGAAGTCAGAGAAA+TGG + chr3.3:8524454-8524473 MS.gene069310:CDS 40.0%
AACAGATGGAGCTTCCAGAA+TGG + chr3.3:8524167-8524186 MS.gene069310:intron 45.0%
CATGGTTTCATCTCACGTGT+TGG + chr3.3:8523961-8523980 MS.gene069310:intron 45.0%
CCTTATTGAGATGGACACCA+AGG + chr3.3:8524552-8524571 MS.gene069310:CDS 45.0%
GACTGCAAGGTTTAAGGAGT+TGG + chr3.3:8524204-8524223 MS.gene069310:CDS 45.0%
GACTTCCACCATAAATCCTC+TGG - chr3.3:8524447-8524466 None:intergenic 45.0%
GCATTCCAGAGGATTTATGG+TGG + chr3.3:8524439-8524458 MS.gene069310:CDS 45.0%
GGTGCATTCCAGAGGATTTA+TGG + chr3.3:8524436-8524455 MS.gene069310:CDS 45.0%
TAGTACCAGTCAGGGTCATA+AGG - chr3.3:8524232-8524251 None:intergenic 45.0%
TTGAAGAATGTGGCGAGCAA+TGG - chr3.3:8524515-8524534 None:intergenic 45.0%
!! CCTTGGTGTCCATCTCAATA+AGG - chr3.3:8524555-8524574 None:intergenic 45.0%
ACCCCTCAAGTAGATCTTCC+TGG - chr3.3:8524404-8524423 None:intergenic 50.0%
AGGAAGATCTACTTGAGGGG+TGG + chr3.3:8524403-8524422 MS.gene069310:CDS 50.0%
CAAGTAGATCTTCCTGGCCA+TGG - chr3.3:8524398-8524417 None:intergenic 50.0%
CCACTGCTCTTGCAGAAATG+AGG - chr3.3:8524490-8524509 None:intergenic 50.0%
CCTCATTTCTGCAAGAGCAG+TGG + chr3.3:8524487-8524506 MS.gene069310:CDS 50.0%
CTCTGACGTAGTACCAGTCA+GGG - chr3.3:8524240-8524259 None:intergenic 50.0%
CTGCTCTTGCAGAAATGAGG+TGG - chr3.3:8524487-8524506 None:intergenic 50.0%
GCCAGGAAGATCTACTTGAG+GGG + chr3.3:8524400-8524419 MS.gene069310:CDS 50.0%
GGCCAGGAAGATCTACTTGA+GGG + chr3.3:8524399-8524418 MS.gene069310:CDS 50.0%
TGGCCAGGAAGATCTACTTG+AGG + chr3.3:8524398-8524417 MS.gene069310:CDS 50.0%
! ATCTACTTGAGGGGTGGACT+CGG + chr3.3:8524409-8524428 MS.gene069310:CDS 50.0%
! TTTTTGTGCCGCAGCTTCCA+TGG + chr3.3:8524378-8524397 MS.gene069310:intron 50.0%
GACTGGTACTACGTCAGAGC+TGG + chr3.3:8524241-8524260 MS.gene069310:CDS 55.0%
GCTCTGACGTAGTACCAGTC+AGG - chr3.3:8524241-8524260 None:intergenic 55.0%
TGGCTCCTTATGACCCTGAC+TGG + chr3.3:8524224-8524243 MS.gene069310:CDS 55.0%
!! TCGGTGTTGGTGCATTCCAG+AGG + chr3.3:8524428-8524447 MS.gene069310:CDS 55.0%
CTTCCTGGCCATGGAAGCTG+CGG - chr3.3:8524389-8524408 None:intergenic 60.0%
!! TTGAGGGGTGGACTCGGTGT+TGG + chr3.3:8524415-8524434 MS.gene069310:CDS 60.0%
GTGCCGCAGCTTCCATGGCC+AGG + chr3.3:8524383-8524402 MS.gene069310:intron 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 8523205 8524579 8523205 ID=MS.gene069310
chr3.3 mRNA 8523205 8524579 8523205 ID=MS.gene069310.t1;Parent=MS.gene069310
chr3.3 exon 8523205 8523288 8523205 ID=MS.gene069310.t1.exon1;Parent=MS.gene069310.t1
chr3.3 CDS 8523205 8523288 8523205 ID=cds.MS.gene069310.t1;Parent=MS.gene069310.t1
chr3.3 exon 8524172 8524262 8524172 ID=MS.gene069310.t1.exon2;Parent=MS.gene069310.t1
chr3.3 CDS 8524172 8524262 8524172 ID=cds.MS.gene069310.t1;Parent=MS.gene069310.t1
chr3.3 exon 8524392 8524579 8524392 ID=MS.gene069310.t1.exon3;Parent=MS.gene069310.t1
chr3.3 CDS 8524392 8524579 8524392 ID=cds.MS.gene069310.t1;Parent=MS.gene069310.t1
Gene Sequence

>MS.gene069310

ATGGCTACCGCAAGGACTGTTCAAGATGTTTCTTCACACGAGTTTGTCAAAGCTTACTCTGCTCATCTCAAAAGATCTGGCAAGGTTAATCAATTCACCGAAAAATTTCATTTTGGTTTTCTATTTTGATTGTGTATGATTCGATTTGTTTTGATTTTTTTTTTTGAAGAGGAATTTGTTTTGATTTGCTTAACGGGTTCATGAAATTTTGTTGAATTAATCTGTTTTAGTAGCTTTTTTGTTTTTGTTATTTTTATTTATTTATAAATTGTCATGCTTTATTGTTTAGATTTGTGTTTTTTTGTGTTTTGAATGTTATGTTTTTGGTTGGGTTTGGTTAAAGTTTATAACTTGATCTACTTCGTTGCTGTGTCTTGTTGAATGTAGTTTTGTTTGTCTAATCCTAACAAAAAAATGATTTTGATTTGATTTGATTGGATTAATAAATAGAAAAATGTTCGTTGTGATGTTAATATGCTGTAATTTTTATAAGTGATTGATTCAGAGTAAGCATTTGTCAGAAAACTAGTGACATGATAGATTTGTGATTTTTCTCTTACACTTGTTTTTGATTAGTTTTAGTGAAAACATTCAAGTTGATTATGATTGTTTTTGTTAATCAAATTAGGAATGATTTTCTGTATTATATGATGTTGATGACAGTTTTTATTTTTTTATGCTATGAATTGTAATGTCCCTTTTGAACTTTCAAAATTTGTTGTTGGATCCTTATTCTAATTACATAGATTACTTGGGCATGGTTTCATCTCACGTGTTGGCTTAGTTTTAGAAATAACTTTCATTATGCTTTTGATTTTTTATGTATTATGTATTCAAGCTCTACTTCACTGGTGTGATCTAGTTTATAATGATACGAATGAAATGGCCGTGTAATTGTATAGTTCAAATTTAGTTGGATTATTATTCTAATTATAGTTTTTTGTTTTCCTTATTTTGTTATGAACAGATGGAGCTTCCAGAATGGACAGATATTGTGAAGACTGCAAGGTTTAAGGAGTTGGCTCCTTATGACCCTGACTGGTACTACGTCAGAGCTGGTGAGTTTCTTGTGTTTGATTTTGTGCATTTCCTGGTCCCTTGTTTAAATTAACAAAAAAATAACTTTCGAGGGAACATTTTTTGATCAACCTGTTGTTGAAAATTGCAATGTGTTTTTTGTGCCGCAGCTTCCATGGCCAGGAAGATCTACTTGAGGGGTGGACTCGGTGTTGGTGCATTCCAGAGGATTTATGGTGGAAGTCAGAGAAATGGCAGTCGTCCACCTCATTTCTGCAAGAGCAGTGGTTCCATTGCTCGCCACATTCTTCAACAGTTGCAGAACATGAACCTTATTGAGATGGACACCAAGGGGTAA

Protein sequence

>MS.gene069310.t1

MATARTVQDVSSHEFVKAYSAHLKRSGKMELPEWTDIVKTARFKELAPYDPDWYYVRAASMARKIYLRGGLGVGAFQRIYGGSQRNGSRPPHFCKSSGSIARHILQQLQNMNLIEMDTKG