Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061296.t1 | XP_013455692.1 | 96 | 50 | 2 | 0 | 100 | 149 | 103 | 152 | 5.20E-18 | 100.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061296.t1 | A0A072UKU9 | 96.0 | 50 | 2 | 0 | 100 | 149 | 103 | 152 | 3.8e-18 | 100.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049580 | MS.gene061296 | 0.808812 | 2.58E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene061296 | 0.817436 | 3.30E-52 | -1.69E-46 |
| MS.gene050559 | MS.gene061296 | 0.802167 | 6.38E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene061296 | -0.806016 | 1.01E-49 | -1.69E-46 |
| MS.gene052358 | MS.gene061296 | -0.813585 | 2.38E-51 | -1.69E-46 |
| MS.gene052586 | MS.gene061296 | 0.810722 | 1.00E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene061296 | 0.809694 | 1.67E-50 | -1.69E-46 |
| MS.gene053188 | MS.gene061296 | 0.805479 | 1.31E-49 | -1.69E-46 |
| MS.gene053216 | MS.gene061296 | 0.801095 | 1.06E-48 | -1.69E-46 |
| MS.gene053294 | MS.gene061296 | 0.809385 | 1.94E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene061296 | 0.811524 | 6.70E-51 | -1.69E-46 |
| MS.gene053995 | MS.gene061296 | 0.803413 | 3.53E-49 | -1.69E-46 |
| MS.gene05548 | MS.gene061296 | 0.856475 | 3.14E-62 | -1.69E-46 |
| MS.gene057079 | MS.gene061296 | 0.828332 | 9.54E-55 | -1.69E-46 |
| MS.gene057287 | MS.gene061296 | 0.816894 | 4.37E-52 | -1.69E-46 |
| MS.gene05795 | MS.gene061296 | 0.808322 | 3.28E-50 | -1.69E-46 |
| MS.gene058074 | MS.gene061296 | 0.804654 | 1.95E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene061296 | 0.800246 | 1.58E-48 | -1.69E-46 |
| MS.gene059317 | MS.gene061296 | 0.815703 | 8.07E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene061296 | 0.82154 | 3.82E-53 | -1.69E-46 |
| MS.gene060586 | MS.gene061296 | 0.802514 | 5.41E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene061296 | 0.813312 | 2.73E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene061296 | -0.800656 | 1.30E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061296.t1 | MTR_4g050130 | 88.435 | 147 | 17 | 0 | 3 | 149 | 6 | 152 | 4.04e-57 | 175 |
| MS.gene061296.t1 | MTR_2g087830 | 38.519 | 135 | 66 | 4 | 15 | 148 | 8 | 126 | 9.98e-26 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061296.t1 | AT3G11690 | 34.783 | 161 | 83 | 4 | 10 | 148 | 41 | 201 | 6.96e-18 | 77.8 |
| MS.gene061296.t1 | AT5G06380 | 36.429 | 140 | 75 | 2 | 10 | 148 | 12 | 138 | 3.32e-15 | 68.6 |
| MS.gene061296.t1 | AT2G27180 | 41.481 | 135 | 68 | 2 | 17 | 148 | 33 | 159 | 1.45e-13 | 64.7 |
Find 34 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGCGGGAACCGGGTTT+TGG | 0.261947 | 4.2:-51569569 | MS.gene061296:CDS |
| TGGGCACGGTTTGCGGGAAC+CGG | 0.294255 | 4.2:-51569576 | MS.gene061296:CDS |
| GAGATGTGGGCACGGTTTGC+GGG | 0.342993 | 4.2:-51569582 | MS.gene061296:CDS |
| CAGAGATCAAGCAGTGTTGC+TGG | 0.387892 | 4.2:-51569693 | MS.gene061296:CDS |
| TCGTGCGGCGGAGGAGAAAT+CGG | 0.423161 | 4.2:-51569625 | MS.gene061296:CDS |
| GGAGATGTGGGCACGGTTTG+CGG | 0.441228 | 4.2:-51569583 | MS.gene061296:CDS |
| GGGCACGGTTTGCGGGAACC+GGG | 0.458010 | 4.2:-51569575 | MS.gene061296:CDS |
| TGATATTAAGATTGATTCGT+TGG | 0.462200 | 4.2:-51569667 | MS.gene061296:CDS |
| CGTGAAGGATCGTGCGGCGG+AGG | 0.484879 | 4.2:-51569634 | MS.gene061296:CDS |
| CGCGGCGTTGGCGAAACAGC+AGG | 0.485789 | 4.2:-51569915 | MS.gene061296:CDS |
| TGAATGAGTGAGAGCGTGGC+TGG | 0.527453 | 4.2:+51569962 | None:intergenic |
| GAAATCGGAGGAGGTTGCGT+TGG | 0.533846 | 4.2:-51569610 | MS.gene061296:CDS |
| TTTGAAAAGTGCGGTCGGAA+CGG | 0.541545 | 4.2:+51569812 | None:intergenic |
| CCTACGTCGTCTTCGATGAA+CGG | 0.547897 | 4.2:+51569785 | None:intergenic |
| CGTGGTAAAAGACGCGGCGT+TGG | 0.551905 | 4.2:-51569927 | MS.gene061296:CDS |
| GGAGGAGGTTGCGTTGGAGA+AGG | 0.558865 | 4.2:-51569604 | MS.gene061296:CDS |
| GCATGGACAGCAACAGCATA+CGG | 0.560127 | 4.2:+51569869 | None:intergenic |
| AGAGATCAAGCAGTGTTGCT+GGG | 0.568754 | 4.2:-51569692 | MS.gene061296:CDS |
| TGCGTTGGAGAAGGAGATGT+GGG | 0.586252 | 4.2:-51569595 | MS.gene061296:CDS |
| ACGACGTTGAGAACCGTGCA+TGG | 0.594359 | 4.2:+51569852 | None:intergenic |
| ATGCTGTTGCTGTCCATGCA+CGG | 0.601793 | 4.2:-51569865 | MS.gene061296:CDS |
| GGCGGAGGAGAAATCGGAGG+AGG | 0.610740 | 4.2:-51569619 | MS.gene061296:CDS |
| AGAATCACTTCTCCAAAACC+CGG | 0.610774 | 4.2:+51569557 | None:intergenic |
| GTTGGAAGAGCATTTCGTGA+AGG | 0.624024 | 4.2:-51569649 | MS.gene061296:CDS |
| TTGCGTTGGAGAAGGAGATG+TGG | 0.625295 | 4.2:-51569596 | MS.gene061296:CDS |
| TGGAGAAGGAGATGTGGGCA+CGG | 0.638141 | 4.2:-51569590 | MS.gene061296:CDS |
| TTTCGTGAAGGATCGTGCGG+CGG | 0.669525 | 4.2:-51569637 | MS.gene061296:CDS |
| CCGTTCATCGAAGACGACGT+AGG | 0.674199 | 4.2:-51569785 | MS.gene061296:CDS |
| TGCGGCGGAGGAGAAATCGG+AGG | 0.691203 | 4.2:-51569622 | MS.gene061296:CDS |
| GACGTGAATGAGTGAGAGCG+TGG | 0.698600 | 4.2:+51569958 | None:intergenic |
| ACGCCGCGTGGTAAAAGACG+CGG | 0.702345 | 4.2:-51569933 | MS.gene061296:CDS |
| CATTCACGTCCTACGCCGCG+TGG | 0.702656 | 4.2:-51569945 | MS.gene061296:CDS |
| GCATTTCGTGAAGGATCGTG+CGG | 0.703359 | 4.2:-51569640 | MS.gene061296:CDS |
| TCGGAACGGAGTAGACAGCG+AGG | 0.793327 | 4.2:+51569826 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATATTAAGATTGATTCGT+TGG | - | chr4.2:51569844-51569863 | MS.gene061296:CDS | 25.0% |
| !!! | AACGGCTTTTTTGAAAAGTG+CGG | + | chr4.2:51569711-51569730 | None:intergenic | 35.0% |
| AGAATCACTTCTCCAAAACC+CGG | + | chr4.2:51569957-51569976 | None:intergenic | 40.0% | |
| GAAAAACAACGTTAACGACG+TGG | - | chr4.2:51569781-51569800 | MS.gene061296:CDS | 40.0% | |
| !!! | GCTTTTTTGAAAAGTGCGGT+CGG | + | chr4.2:51569707-51569726 | None:intergenic | 40.0% |
| ! | AGAGATCAAGCAGTGTTGCT+GGG | - | chr4.2:51569819-51569838 | MS.gene061296:CDS | 45.0% |
| ! | TTTGAAAAGTGCGGTCGGAA+CGG | + | chr4.2:51569702-51569721 | None:intergenic | 45.0% |
| !! | GTTGGAAGAGCATTTCGTGA+AGG | - | chr4.2:51569862-51569881 | MS.gene061296:CDS | 45.0% |
| ATGCTGTTGCTGTCCATGCA+CGG | - | chr4.2:51569646-51569665 | MS.gene061296:CDS | 50.0% | |
| CAGAGATCAAGCAGTGTTGC+TGG | - | chr4.2:51569818-51569837 | MS.gene061296:CDS | 50.0% | |
| CCTACGTCGTCTTCGATGAA+CGG | + | chr4.2:51569729-51569748 | None:intergenic | 50.0% | |
| GCATGGACAGCAACAGCATA+CGG | + | chr4.2:51569645-51569664 | None:intergenic | 50.0% | |
| GCATTTCGTGAAGGATCGTG+CGG | - | chr4.2:51569871-51569890 | MS.gene061296:CDS | 50.0% | |
| TGCGTTGGAGAAGGAGATGT+GGG | - | chr4.2:51569916-51569935 | MS.gene061296:CDS | 50.0% | |
| TTGCGTTGGAGAAGGAGATG+TGG | - | chr4.2:51569915-51569934 | MS.gene061296:CDS | 50.0% | |
| ACGACGTTGAGAACCGTGCA+TGG | + | chr4.2:51569662-51569681 | None:intergenic | 55.0% | |
| CCGTTCATCGAAGACGACGT+AGG | - | chr4.2:51569726-51569745 | MS.gene061296:CDS | 55.0% | |
| GAAATCGGAGGAGGTTGCGT+TGG | - | chr4.2:51569901-51569920 | MS.gene061296:CDS | 55.0% | |
| GACGTGAATGAGTGAGAGCG+TGG | + | chr4.2:51569556-51569575 | None:intergenic | 55.0% | |
| TGGAGAAGGAGATGTGGGCA+CGG | - | chr4.2:51569921-51569940 | MS.gene061296:CDS | 55.0% | |
| TTTCGTGAAGGATCGTGCGG+CGG | - | chr4.2:51569874-51569893 | MS.gene061296:CDS | 55.0% | |
| ! | TGAATGAGTGAGAGCGTGGC+TGG | + | chr4.2:51569552-51569571 | None:intergenic | 55.0% |
| ACGCCGCGTGGTAAAAGACG+CGG | - | chr4.2:51569578-51569597 | MS.gene061296:CDS | 60.0% | |
| CGTGGTAAAAGACGCGGCGT+TGG | - | chr4.2:51569584-51569603 | MS.gene061296:CDS | 60.0% | |
| GAGATGTGGGCACGGTTTGC+GGG | - | chr4.2:51569929-51569948 | MS.gene061296:CDS | 60.0% | |
| GGAGATGTGGGCACGGTTTG+CGG | - | chr4.2:51569928-51569947 | MS.gene061296:CDS | 60.0% | |
| GGAGGAGGTTGCGTTGGAGA+AGG | - | chr4.2:51569907-51569926 | MS.gene061296:CDS | 60.0% | |
| TCGGAACGGAGTAGACAGCG+AGG | + | chr4.2:51569688-51569707 | None:intergenic | 60.0% | |
| TCGTGCGGCGGAGGAGAAAT+CGG | - | chr4.2:51569886-51569905 | MS.gene061296:CDS | 60.0% | |
| ! | ACGCCGCGTCTTTTACCACG+CGG | + | chr4.2:51569584-51569603 | None:intergenic | 60.0% |
| ! | CGTCTTTTACCACGCGGCGT+AGG | + | chr4.2:51569578-51569597 | None:intergenic | 60.0% |
| ! | GGTTTGCGGGAACCGGGTTT+TGG | - | chr4.2:51569942-51569961 | MS.gene061296:CDS | 60.0% |
| CATTCACGTCCTACGCCGCG+TGG | - | chr4.2:51569566-51569585 | MS.gene061296:CDS | 65.0% | |
| GGCGGAGGAGAAATCGGAGG+AGG | - | chr4.2:51569892-51569911 | MS.gene061296:CDS | 65.0% | |
| TGCGGCGGAGGAGAAATCGG+AGG | - | chr4.2:51569889-51569908 | MS.gene061296:CDS | 65.0% | |
| TGGGCACGGTTTGCGGGAAC+CGG | - | chr4.2:51569935-51569954 | MS.gene061296:CDS | 65.0% | |
| CGTGAAGGATCGTGCGGCGG+AGG | - | chr4.2:51569877-51569896 | MS.gene061296:CDS | 70.0% | |
| GGGCACGGTTTGCGGGAACC+GGG | - | chr4.2:51569936-51569955 | MS.gene061296:CDS | 70.0% | |
| !! | CGCGGCGTTGGCGAAACAGC+AGG | - | chr4.2:51569596-51569615 | MS.gene061296:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 51569542 | 51569991 | 51569542 | ID=MS.gene061296 |
| chr4.2 | mRNA | 51569542 | 51569991 | 51569542 | ID=MS.gene061296.t1;Parent=MS.gene061296 |
| chr4.2 | exon | 51569542 | 51569991 | 51569542 | ID=MS.gene061296.t1.exon1;Parent=MS.gene061296.t1 |
| chr4.2 | CDS | 51569542 | 51569991 | 51569542 | ID=cds.MS.gene061296.t1;Parent=MS.gene061296.t1 |
| Gene Sequence |
| Protein sequence |
