AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062655

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062655.t1 MTR_6g065400 93.403 288 19 0 1 288 1 288 0.0 559
MS.gene062655.t1 MTR_6g065370 92.361 288 22 0 1 288 1 288 0.0 553
MS.gene062655.t1 MTR_6g065270 78.246 285 58 1 8 288 6 290 9.92e-168 468
MS.gene062655.t1 MTR_6g065120 77.431 288 64 1 2 288 3 290 1.29e-166 466
MS.gene062655.t1 MTR_6g065340 75.610 287 54 1 2 288 13 283 1.17e-151 427
MS.gene062655.t1 MTR_6g065110 70.423 284 84 0 5 288 6 289 2.07e-150 424
MS.gene062655.t1 MTR_6g065430 58.451 284 109 2 7 288 6 282 6.13e-118 342
MS.gene062655.t1 MTR_1g082210 42.268 291 161 2 1 287 7 294 3.25e-75 233
MS.gene062655.t1 MTR_1g089310 43.686 293 154 4 1 287 7 294 2.16e-74 231
MS.gene062655.t1 MTR_1g089310 43.686 293 154 4 1 287 23 310 3.27e-74 231
MS.gene062655.t1 MTR_7g093950 41.017 295 165 4 1 287 7 300 7.13e-72 225
MS.gene062655.t1 MTR_6g464280 39.552 268 142 6 32 289 24 281 1.10e-54 180
MS.gene062655.t1 MTR_6g464510 37.698 252 140 6 47 289 40 283 5.00e-49 166
MS.gene062655.t1 MTR_8g035540 37.698 252 138 4 47 288 30 272 1.91e-48 164
MS.gene062655.t1 MTR_0007s0520 37.786 262 139 6 1 256 1 244 6.39e-46 157
MS.gene062655.t1 MTR_1g102160 35.612 278 142 9 31 289 23 282 5.42e-45 155
MS.gene062655.t1 MTR_6g464320 36.940 268 149 6 32 289 25 282 6.27e-45 155
MS.gene062655.t1 MTR_1g102170 36.752 234 122 5 32 256 43 259 4.08e-43 150
MS.gene062655.t1 MTR_6g464120 37.089 213 124 4 47 256 41 246 7.78e-42 151
MS.gene062655.t1 MTR_8g035520 35.200 250 143 5 49 288 40 280 1.12e-41 146
MS.gene062655.t1 MTR_1g012430 35.938 256 146 6 31 283 15 255 1.48e-40 143
MS.gene062655.t1 MTR_4g050140 33.969 262 152 4 32 285 18 266 1.22e-37 135
MS.gene062655.t1 MTR_8g035500 30.970 268 165 6 32 289 17 274 5.68e-37 134
MS.gene062655.t1 MTR_7g406880 36.000 250 135 8 55 285 54 297 7.35e-37 134
MS.gene062655.t1 MTR_1g111280 33.730 252 140 8 48 285 52 290 1.07e-36 134
MS.gene062655.t1 MTR_7g107040 32.517 286 161 6 10 285 4 267 1.41e-36 133
MS.gene062655.t1 MTR_4g086510 33.333 222 117 5 49 256 35 239 3.37e-30 116
MS.gene062655.t1 MTR_7g056207 33.180 217 124 4 49 256 67 271 4.95e-30 116
MS.gene062655.t1 MTR_3g012440 31.967 244 160 1 48 285 41 284 8.45e-30 115
MS.gene062655.t1 MTR_4g086540 34.054 185 111 3 38 220 33 208 1.02e-29 114
MS.gene062655.t1 MTR_4g086480 30.864 243 148 4 49 285 39 267 1.09e-29 114
MS.gene062655.t1 MTR_3g101420 30.364 247 145 5 49 285 34 263 1.84e-29 114
MS.gene062655.t1 MTR_6g022710 34.483 203 96 3 91 256 156 358 8.86e-28 111
MS.gene062655.t1 MTR_4g086470 30.453 243 149 4 49 285 38 266 2.08e-27 108
MS.gene062655.t1 MTR_6g065470 36.842 171 76 3 7 159 6 162 4.93e-27 103
MS.gene062655.t1 MTR_5g005920 28.897 263 165 8 32 284 26 276 2.29e-26 106
MS.gene062655.t1 MTR_3g450680 28.968 252 141 7 53 285 48 280 1.77e-24 100
MS.gene062655.t1 MTR_8g040860 26.718 262 146 8 46 283 36 275 2.25e-22 95.1
MS.gene062655.t1 MTR_1g104930 31.933 238 129 6 59 281 48 267 1.49e-21 92.8
MS.gene062655.t1 MTR_1g105020 27.138 269 163 9 32 285 21 271 1.96e-21 92.4
MS.gene062655.t1 MTR_5g005900 27.473 273 160 11 32 285 22 275 2.80e-21 92.0
MS.gene062655.t1 MTR_5g005850 28.862 246 149 7 53 285 45 277 2.35e-20 89.7
MS.gene062655.t1 MTR_7g051020 31.474 251 141 7 48 283 38 272 1.25e-19 87.4
MS.gene062655.t1 MTR_1g104933 31.356 236 133 6 59 283 46 263 3.17e-18 83.6
MS.gene062655.t1 MTR_0007s0480 40.659 91 48 2 171 256 1 90 1.68e-14 70.5
MS.gene062655.t1 MTR_6g065480 50.877 57 27 1 233 289 1 56 3.57e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062655.t1 AT5G23530 55.282 284 109 5 10 289 13 282 4.79e-112 327
MS.gene062655.t1 AT5G27320 43.310 284 155 2 7 287 13 293 6.49e-73 228
MS.gene062655.t1 AT3G63010 40.956 293 163 3 1 287 7 295 3.01e-70 221
MS.gene062655.t1 AT3G05120 41.259 286 160 2 7 287 13 295 1.03e-67 214
MS.gene062655.t1 AT5G62180 38.403 263 152 6 32 288 21 279 2.97e-54 179
MS.gene062655.t1 AT2G45600 33.740 246 136 5 58 288 38 271 2.34e-44 153
MS.gene062655.t1 AT5G16080 36.842 247 132 6 48 283 54 287 8.23e-43 150
MS.gene062655.t1 AT1G68620 37.447 235 131 5 56 285 57 280 1.35e-42 149
MS.gene062655.t1 AT2G45610 31.955 266 158 4 1 259 1 250 2.22e-42 148
MS.gene062655.t1 AT1G19190 33.333 261 147 8 32 283 18 260 1.56e-35 130
MS.gene062655.t1 AT5G06570 39.331 239 125 5 60 288 50 278 3.15e-35 129
MS.gene062655.t1 AT5G06570 39.331 239 125 5 60 288 50 278 3.15e-35 129
MS.gene062655.t1 AT5G06570 39.331 239 125 5 60 288 65 293 3.31e-35 130
MS.gene062655.t1 AT5G06570 39.331 239 125 5 60 288 65 293 3.31e-35 130
MS.gene062655.t1 AT3G48700 32.271 251 145 6 49 284 32 272 1.32e-32 122
MS.gene062655.t1 AT3G48690 32.937 252 138 6 49 284 32 268 1.45e-31 120
MS.gene062655.t1 AT2G03550 35.270 241 131 7 49 281 32 255 1.64e-31 119
MS.gene062655.t1 AT5G14310 33.871 248 121 4 81 285 135 382 1.69e-31 122
MS.gene062655.t1 AT1G47480 30.435 276 160 7 9 283 12 256 4.74e-30 115
MS.gene062655.t1 AT3G27320 28.028 289 129 5 47 256 48 336 1.16e-26 108
MS.gene062655.t1 AT3G27320 30.579 242 119 5 56 256 135 368 3.46e-26 107
MS.gene062655.t1 AT1G49640 32.275 189 118 5 30 217 17 196 3.68e-25 102
MS.gene062655.t1 AT1G49660 31.799 239 147 6 53 284 36 265 7.46e-25 101
MS.gene062655.t1 AT1G49650 30.932 236 146 6 53 281 92 317 1.88e-23 99.0

Find 95 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGAGGATTTGTCGGGTT+TGG 0.228528 6.4:+46161173 MS.gene062655:CDS
ACCCGATAACCCGGATATTC+TGG 0.265541 6.4:-46160984 None:intergenic
GACAGGAAGGGAGGTTGTTT+TGG 0.266002 6.4:-46160659 None:intergenic
AGATAGCGCCGGTGGAAATT+TGG 0.269758 6.4:+46160924 MS.gene062655:CDS
CTTCCAAGGAAGAAGGGGTT+TGG 0.297350 6.4:-46160407 None:intergenic
AGCGTTAACGGTAACGTCTT+TGG 0.321214 6.4:-46160566 None:intergenic
GCGGTTAGGGCTTGCAAAGC+GGG 0.337635 6.4:+46160958 MS.gene062655:CDS
GGCACACCATGTTGCGGTTA+GGG 0.353563 6.4:+46160945 MS.gene062655:CDS
CTCCAGAATATCCGGGTTAT+CGG 0.354754 6.4:+46160982 MS.gene062655:CDS
GGTTTGATCCTTTGATTGAT+TGG 0.363142 6.4:+46161226 MS.gene062655:CDS
TGGCACACCATGTTGCGGTT+AGG 0.375335 6.4:+46160944 MS.gene062655:CDS
AACCCAAACCCCTTCTTCCT+TGG 0.375869 6.4:+46160404 MS.gene062655:CDS
AAGCGGGGCTCCAGAATATC+CGG 0.405006 6.4:+46160974 MS.gene062655:CDS
TTCCAAGGAAGAAGGGGTTT+GGG 0.406480 6.4:-46160406 None:intergenic
GTCGAACGCGGAGGATTTGT+CGG 0.408612 6.4:+46161167 MS.gene062655:CDS
TTTGGTGGAAACGCCGTTAA+CGG 0.432566 6.4:-46160548 None:intergenic
CCTAACGCCACTCCCGTTAA+CGG 0.446230 6.4:+46160535 MS.gene062655:CDS
TTGGTGGAAACGCCGTTAAC+GGG 0.454826 6.4:-46160547 None:intergenic
TTTGTCGGGTTTGGATTATC+CGG 0.455467 6.4:+46161182 MS.gene062655:CDS
TTTGATAAGTCAGCATTTCC+CGG 0.460115 6.4:-46160886 None:intergenic
TAAAACTGTAAGAAGTGAAA+TGG 0.461001 6.4:-46160440 None:intergenic
TTAACGCTGAAAATAACGTT+TGG 0.461518 6.4:+46160581 MS.gene062655:CDS
TCCAGAATATCCGGGTTATC+GGG 0.461680 6.4:+46160983 MS.gene062655:CDS
TGTCAGTGGCAAGGACTGAT+TGG 0.463526 6.4:+46161082 MS.gene062655:CDS
CATGGCGCGGTTAATGTATG+TGG 0.468536 6.4:+46161144 MS.gene062655:CDS
GAGGGATCGGACCGGGATCA+TGG 0.475304 6.4:+46161126 MS.gene062655:CDS
ATATTGATCCAACCGTTCTT+CGG 0.477926 6.4:+46161009 MS.gene062655:CDS
AGGAGAAGAACGGACGGAGG+CGG 0.478435 6.4:+46161032 MS.gene062655:CDS
CGCGGACCTTCCAAGGAAGA+AGG 0.480629 6.4:-46160414 None:intergenic
CGTTCTTCTCCTCCGAAGAA+CGG 0.480969 6.4:-46161021 None:intergenic
CCGTTAACGGGAGTGGCGTT+AGG 0.486028 6.4:-46160535 None:intergenic
TTTGGTGGAACCACCATCTC+CGG 0.486902 6.4:-46160641 None:intergenic
CTTTGATTGATTGGCAAAAG+AGG 0.488937 6.4:+46161235 MS.gene062655:CDS
CTTCTCCTCCGAAGAACGGT+TGG 0.489442 6.4:-46161017 None:intergenic
TCGAACGCGGAGGATTTGTC+GGG 0.500774 6.4:+46161168 MS.gene062655:CDS
AGGCGGAGATTCATCTAGTT+GGG 0.501879 6.4:+46161049 MS.gene062655:CDS
GAGGCGGAGATTCATCTAGT+TGG 0.506923 6.4:+46161048 MS.gene062655:CDS
GGTGACTTCTTCGGCAACAA+TGG 0.510041 6.4:-46160620 None:intergenic
GTGACTTCTTCGGCAACAAT+GGG 0.510565 6.4:-46160619 None:intergenic
GGAAACGCCGTTAACGGGAG+TGG 0.511016 6.4:-46160542 None:intergenic
ATGGTAGCGCGGACCTTCCA+AGG 0.517263 6.4:-46160421 None:intergenic
GCCGAAGAAGTCACCGGAGA+TGG 0.519352 6.4:+46160628 MS.gene062655:CDS
TGCGACGGATAGCGATGCTC+TGG 0.519802 6.4:-46160808 None:intergenic
TTATCAAAATGTTTCCTAGC+CGG 0.525235 6.4:+46160901 MS.gene062655:CDS
ATTTCCACCGGCGCTATCTC+CGG 0.529772 6.4:-46160920 None:intergenic
CATTAACCGCGCCATGATCC+CGG 0.530924 6.4:-46161137 None:intergenic
ACCATCTCCGGTGACTTCTT+CGG 0.531633 6.4:-46160629 None:intergenic
GTCCTTGCCACTGACACAAA+CGG 0.534029 6.4:-46161075 None:intergenic
AACGGGAGTGGCGTTAGGTG+AGG 0.535479 6.4:-46160530 None:intergenic
AACAGCATCGTAGAGGTTTG+AGG 0.537092 6.4:-46160725 None:intergenic
GCGGACCTTCCAAGGAAGAA+GGG 0.545035 6.4:-46160413 None:intergenic
TTTACCGGAGGGATCGGACC+GGG 0.545728 6.4:+46161119 MS.gene062655:CDS
GTTAAAGAGACGGCGGTTGA+CGG 0.545760 6.4:-46160491 None:intergenic
CAAACCCCTTCTTCCTTGGA+AGG 0.548828 6.4:+46160408 MS.gene062655:CDS
TGGGATGCCGTTTGTGTCAG+TGG 0.550089 6.4:+46161068 MS.gene062655:CDS
TGCCGTTTGTGTCAGTGGCA+AGG 0.550679 6.4:+46161073 MS.gene062655:CDS
TGATCCCGGTCCGATCCCTC+CGG 0.553708 6.4:-46161123 None:intergenic
TGCGGTTAGGGCTTGCAAAG+CGG 0.562566 6.4:+46160957 MS.gene062655:CDS
TCAATATTGACCCGATAACC+CGG 0.567192 6.4:-46160993 None:intergenic
CGGTTAGGGCTTGCAAAGCG+GGG 0.572111 6.4:+46160959 MS.gene062655:CDS
CCGTGGAAGAAAATGATGAC+AGG 0.580978 6.4:-46160676 None:intergenic
TGGCAAGGACTGATTGGATG+TGG 0.586260 6.4:+46161088 MS.gene062655:CDS
GAAAATGATGACAGGAAGGG+AGG 0.591882 6.4:-46160668 None:intergenic
GGAAGAAAATGATGACAGGA+AGG 0.592101 6.4:-46160672 None:intergenic
GATGCTTCATGTCGAGCTAA+CGG 0.595030 6.4:+46160466 MS.gene062655:CDS
GTTAACGGTAACGTCTTTGG+TGG 0.595706 6.4:-46160563 None:intergenic
CACCGGCGCTATCTCCGGCT+AGG 0.596783 6.4:-46160915 None:intergenic
AGCGGGGCTCCAGAATATCC+GGG 0.603800 6.4:+46160975 MS.gene062655:CDS
GAAGAAGTCACCGGAGATGG+TGG 0.605947 6.4:+46160631 MS.gene062655:CDS
ATTGTTGCCGAAGAAGTCAC+CGG 0.606124 6.4:+46160622 MS.gene062655:CDS
CTAGCCGGAGATAGCGCCGG+TGG 0.609370 6.4:+46160916 MS.gene062655:CDS
ATGGCGCGGTTAATGTATGT+GGG 0.611682 6.4:+46161145 MS.gene062655:CDS
AAATTTGGCACACCATGTTG+CGG 0.611772 6.4:+46160939 MS.gene062655:CDS
TTGATCCAACCGTTCTTCGG+AGG 0.612628 6.4:+46161012 MS.gene062655:CDS
ATGGTGTGCCAAATTTCCAC+CGG 0.614422 6.4:-46160932 None:intergenic
TCTCCGTCTTCATATTGCGA+CGG 0.615711 6.4:-46160823 None:intergenic
CGGACCTTCCAAGGAAGAAG+GGG 0.620041 6.4:-46160412 None:intergenic
TGCAAGCCCTAACCGCAACA+TGG 0.630176 6.4:-46160951 None:intergenic
GAAAAGTGAAGCCACCGCCG+TGG 0.637720 6.4:-46160693 None:intergenic
CAACAATGGGAGTAAAGAGA+CGG 0.642848 6.4:-46160606 None:intergenic
TAATGTATGTGGGTCGAACG+CGG 0.643618 6.4:+46161155 MS.gene062655:CDS
GATCGAAGAAGTTAAAGAGA+CGG 0.645752 6.4:-46160501 None:intergenic
AGCGATGCTCTGGTGCAAGT+CGG 0.647802 6.4:-46160798 None:intergenic
ATCGGACCGGGATCATGGCG+CGG 0.650554 6.4:+46161131 MS.gene062655:CDS
GAAGAAAATGATGACAGGAA+GGG 0.651353 6.4:-46160671 None:intergenic
CGTTCTTCGGAGGAGAAGAA+CGG 0.653860 6.4:+46161022 MS.gene062655:CDS
TACGGCAAACAGCATCGTAG+AGG 0.655570 6.4:-46160732 None:intergenic
CTTCGGAGGAGAAGAACGGA+CGG 0.656110 6.4:+46161026 MS.gene062655:CDS
TATCCGTCGCAATATGAAGA+CGG 0.658300 6.4:+46160820 MS.gene062655:CDS
CGGAGGAGAAGAACGGACGG+AGG 0.662414 6.4:+46161029 MS.gene062655:CDS
TTCCTAGCCGGAGATAGCGC+CGG 0.669385 6.4:+46160913 MS.gene062655:CDS
GTTGACTGAGACGACGACAG+CGG 0.701344 6.4:-46160773 None:intergenic
TAAGAAGTGAAATGGTAGCG+CGG 0.709848 6.4:-46160432 None:intergenic
CGAAGAAGTTAAAGAGACGG+CGG 0.716329 6.4:-46160498 None:intergenic
TGTATGTGGGTCGAACGCGG+AGG 0.725919 6.4:+46161158 MS.gene062655:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAAACTGTAAGAAGTGAAA+TGG - chr6.4:46160443-46160462 None:intergenic 25.0%
!! ATGATTGGTTGAAAAAATGT+GGG + chr6.4:46161262-46161281 MS.gene062655:intron 25.0%
!! TATGATTGGTTGAAAAAATG+TGG + chr6.4:46161261-46161280 MS.gene062655:intron 25.0%
GTTGATTGAATATCCAAACA+TGG + chr6.4:46161296-46161315 MS.gene062655:intron 30.0%
TTAACGCTGAAAATAACGTT+TGG + chr6.4:46160581-46160600 MS.gene062655:CDS 30.0%
TTATCAAAATGTTTCCTAGC+CGG + chr6.4:46160901-46160920 MS.gene062655:CDS 30.0%
!! AACGTTATTTTCAGCGTTAA+CGG - chr6.4:46160581-46160600 None:intergenic 30.0%
ATATTGATCCAACCGTTCTT+CGG + chr6.4:46161009-46161028 MS.gene062655:CDS 35.0%
CTTTGATTGATTGGCAAAAG+AGG + chr6.4:46161235-46161254 MS.gene062655:CDS 35.0%
GAAGAAAATGATGACAGGAA+GGG - chr6.4:46160674-46160693 None:intergenic 35.0%
GATCGAAGAAGTTAAAGAGA+CGG - chr6.4:46160504-46160523 None:intergenic 35.0%
GGTTTGATCCTTTGATTGAT+TGG + chr6.4:46161226-46161245 MS.gene062655:CDS 35.0%
TGAGTAGATTCAGGAAAATC+AGG - chr6.4:46161342-46161361 None:intergenic 35.0%
TTTGATAAGTCAGCATTTCC+CGG - chr6.4:46160889-46160908 None:intergenic 35.0%
! CTCTTTTGCCAATCAATCAA+AGG - chr6.4:46161237-46161256 None:intergenic 35.0%
! TGAAAAGCATGAACCATGTT+TGG - chr6.4:46161312-46161331 None:intergenic 35.0%
!! GGCAAAAGAGGTATTATGAT+TGG + chr6.4:46161247-46161266 MS.gene062655:intron 35.0%
!!! ATACGCTTGTTTTTGTTGGT+GGG + chr6.4:46161205-46161224 MS.gene062655:CDS 35.0%
!!! GATGTGGAAAGTGTTTTTAC+CGG + chr6.4:46161104-46161123 MS.gene062655:CDS 35.0%
AAATTTGGCACACCATGTTG+CGG + chr6.4:46160939-46160958 MS.gene062655:CDS 40.0%
CAACAATGGGAGTAAAGAGA+CGG - chr6.4:46160609-46160628 None:intergenic 40.0%
CCAACAAAAACAAGCGTATC+CGG - chr6.4:46161204-46161223 None:intergenic 40.0%
GGAAGAAAATGATGACAGGA+AGG - chr6.4:46160675-46160694 None:intergenic 40.0%
TATCCGTCGCAATATGAAGA+CGG + chr6.4:46160820-46160839 MS.gene062655:CDS 40.0%
TCAATATTGACCCGATAACC+CGG - chr6.4:46160996-46161015 None:intergenic 40.0%
TTTGTCGGGTTTGGATTATC+CGG + chr6.4:46161182-46161201 MS.gene062655:CDS 40.0%
! CCTGTCATCATTTTCTTCCA+CGG + chr6.4:46160676-46160695 MS.gene062655:CDS 40.0%
! CGAGAACGTAACAGTTTTAC+CGG + chr6.4:46160867-46160886 MS.gene062655:CDS 40.0%
! GAGAACGTAACAGTTTTACC+GGG + chr6.4:46160868-46160887 MS.gene062655:CDS 40.0%
! TAAGAAGTGAAATGGTAGCG+CGG - chr6.4:46160435-46160454 None:intergenic 40.0%
!!! GATACGCTTGTTTTTGTTGG+TGG + chr6.4:46161204-46161223 MS.gene062655:CDS 40.0%
!!! TGGAAAGTGTTTTTACCGGA+GGG + chr6.4:46161108-46161127 MS.gene062655:CDS 40.0%
AACAGCATCGTAGAGGTTTG+AGG - chr6.4:46160728-46160747 None:intergenic 45.0%
AGCGTTAACGGTAACGTCTT+TGG - chr6.4:46160569-46160588 None:intergenic 45.0%
AGGCGGAGATTCATCTAGTT+GGG + chr6.4:46161049-46161068 MS.gene062655:CDS 45.0%
ATGGCGCGGTTAATGTATGT+GGG + chr6.4:46161145-46161164 MS.gene062655:CDS 45.0%
ATGGTGTGCCAAATTTCCAC+CGG - chr6.4:46160935-46160954 None:intergenic 45.0%
ATTGTTGCCGAAGAAGTCAC+CGG + chr6.4:46160622-46160641 MS.gene062655:CDS 45.0%
CCGTGGAAGAAAATGATGAC+AGG - chr6.4:46160679-46160698 None:intergenic 45.0%
CGAAGAAGTTAAAGAGACGG+CGG - chr6.4:46160501-46160520 None:intergenic 45.0%
CTCCAGAATATCCGGGTTAT+CGG + chr6.4:46160982-46161001 MS.gene062655:CDS 45.0%
GAAAATGATGACAGGAAGGG+AGG - chr6.4:46160671-46160690 None:intergenic 45.0%
GATGCTTCATGTCGAGCTAA+CGG + chr6.4:46160466-46160485 MS.gene062655:CDS 45.0%
GTGACTTCTTCGGCAACAAT+GGG - chr6.4:46160622-46160641 None:intergenic 45.0%
GTTAACGGTAACGTCTTTGG+TGG - chr6.4:46160566-46160585 None:intergenic 45.0%
TAATGTATGTGGGTCGAACG+CGG + chr6.4:46161155-46161174 MS.gene062655:CDS 45.0%
TCCAGAATATCCGGGTTATC+GGG + chr6.4:46160983-46161002 MS.gene062655:CDS 45.0%
TCTCCGTCTTCATATTGCGA+CGG - chr6.4:46160826-46160845 None:intergenic 45.0%
TTCCAAGGAAGAAGGGGTTT+GGG - chr6.4:46160409-46160428 None:intergenic 45.0%
TTTGGTGGAAACGCCGTTAA+CGG - chr6.4:46160551-46160570 None:intergenic 45.0%
! AGATTTTGCGGCAGAGTCTA+CGG - chr6.4:46160753-46160772 None:intergenic 45.0%
! GTCATCATTTTCTTCCACGG+CGG + chr6.4:46160679-46160698 MS.gene062655:CDS 45.0%
!! AGTGTTTTTACCGGAGGGAT+CGG + chr6.4:46161113-46161132 MS.gene062655:CDS 45.0%
!!! CCGGATACGCTTGTTTTTGT+TGG + chr6.4:46161201-46161220 MS.gene062655:CDS 45.0%
!!! GTGGAAAGTGTTTTTACCGG+AGG + chr6.4:46161107-46161126 MS.gene062655:CDS 45.0%
AACCCAAACCCCTTCTTCCT+TGG + chr6.4:46160404-46160423 MS.gene062655:CDS 50.0%
ACCATCTCCGGTGACTTCTT+CGG - chr6.4:46160632-46160651 None:intergenic 50.0%
ACCCGATAACCCGGATATTC+TGG - chr6.4:46160987-46161006 None:intergenic 50.0%
CAAACCCCTTCTTCCTTGGA+AGG + chr6.4:46160408-46160427 MS.gene062655:CDS 50.0%
CATGGCGCGGTTAATGTATG+TGG + chr6.4:46161144-46161163 MS.gene062655:CDS 50.0%
CGTTCTTCGGAGGAGAAGAA+CGG + chr6.4:46161022-46161041 MS.gene062655:CDS 50.0%
CGTTCTTCTCCTCCGAAGAA+CGG - chr6.4:46161024-46161043 None:intergenic 50.0%
CTTCCAAGGAAGAAGGGGTT+TGG - chr6.4:46160410-46160429 None:intergenic 50.0%
GAGGCGGAGATTCATCTAGT+TGG + chr6.4:46161048-46161067 MS.gene062655:CDS 50.0%
GGTGACTTCTTCGGCAACAA+TGG - chr6.4:46160623-46160642 None:intergenic 50.0%
GTCCTTGCCACTGACACAAA+CGG - chr6.4:46161078-46161097 None:intergenic 50.0%
GTTAAAGAGACGGCGGTTGA+CGG - chr6.4:46160494-46160513 None:intergenic 50.0%
TACGGCAAACAGCATCGTAG+AGG - chr6.4:46160735-46160754 None:intergenic 50.0%
TGGCAAGGACTGATTGGATG+TGG + chr6.4:46161088-46161107 MS.gene062655:CDS 50.0%
TGTCAGTGGCAAGGACTGAT+TGG + chr6.4:46161082-46161101 MS.gene062655:CDS 50.0%
TTGATCCAACCGTTCTTCGG+AGG + chr6.4:46161012-46161031 MS.gene062655:CDS 50.0%
TTGGTGGAAACGCCGTTAAC+GGG - chr6.4:46160550-46160569 None:intergenic 50.0%
TTTGGTGGAACCACCATCTC+CGG - chr6.4:46160644-46160663 None:intergenic 50.0%
! ATCATTTTCTTCCACGGCGG+TGG + chr6.4:46160682-46160701 MS.gene062655:CDS 50.0%
! GACAGGAAGGGAGGTTGTTT+TGG - chr6.4:46160662-46160681 None:intergenic 50.0%
!! AGATAGCGCCGGTGGAAATT+TGG + chr6.4:46160924-46160943 MS.gene062655:CDS 50.0%
!!! AGGAAGGGAGGTTGTTTTGG+TGG - chr6.4:46160659-46160678 None:intergenic 50.0%
AAGCGGGGCTCCAGAATATC+CGG + chr6.4:46160974-46160993 MS.gene062655:CDS 55.0%
AGCGATGCTCTGGTGCAAGT+CGG - chr6.4:46160801-46160820 None:intergenic 55.0%
ATTTCCACCGGCGCTATCTC+CGG - chr6.4:46160923-46160942 None:intergenic 55.0%
CATTAACCGCGCCATGATCC+CGG - chr6.4:46161140-46161159 None:intergenic 55.0%
CCTAACGCCACTCCCGTTAA+CGG + chr6.4:46160535-46160554 MS.gene062655:CDS 55.0%
CGGACCTTCCAAGGAAGAAG+GGG - chr6.4:46160415-46160434 None:intergenic 55.0%
CTTCGGAGGAGAAGAACGGA+CGG + chr6.4:46161026-46161045 MS.gene062655:CDS 55.0%
CTTCTCCTCCGAAGAACGGT+TGG - chr6.4:46161020-46161039 None:intergenic 55.0%
GAAGAAGTCACCGGAGATGG+TGG + chr6.4:46160631-46160650 MS.gene062655:CDS 55.0%
GCGGACCTTCCAAGGAAGAA+GGG - chr6.4:46160416-46160435 None:intergenic 55.0%
GGCACACCATGTTGCGGTTA+GGG + chr6.4:46160945-46160964 MS.gene062655:CDS 55.0%
GTCGAACGCGGAGGATTTGT+CGG + chr6.4:46161167-46161186 MS.gene062655:CDS 55.0%
GTTGACTGAGACGACGACAG+CGG - chr6.4:46160776-46160795 None:intergenic 55.0%
TCGAACGCGGAGGATTTGTC+GGG + chr6.4:46161168-46161187 MS.gene062655:CDS 55.0%
TGCAAGCCCTAACCGCAACA+TGG - chr6.4:46160954-46160973 None:intergenic 55.0%
TGCGGTTAGGGCTTGCAAAG+CGG + chr6.4:46160957-46160976 MS.gene062655:CDS 55.0%
TGGCACACCATGTTGCGGTT+AGG + chr6.4:46160944-46160963 MS.gene062655:CDS 55.0%
! CGACGACAGCGGAGATTTTG+CGG - chr6.4:46160765-46160784 None:intergenic 55.0%
! TGCCGTTTGTGTCAGTGGCA+AGG + chr6.4:46161073-46161092 MS.gene062655:CDS 55.0%
! TGGGATGCCGTTTGTGTCAG+TGG + chr6.4:46161068-46161087 MS.gene062655:CDS 55.0%
! TTTTACCGGAGGGATCGGAC+CGG + chr6.4:46161118-46161137 MS.gene062655:CDS 55.0%
AACGGGAGTGGCGTTAGGTG+AGG - chr6.4:46160533-46160552 None:intergenic 60.0%
AGCGGGGCTCCAGAATATCC+GGG + chr6.4:46160975-46160994 MS.gene062655:CDS 60.0%
AGGAGAAGAACGGACGGAGG+CGG + chr6.4:46161032-46161051 MS.gene062655:CDS 60.0%
CCGTTAACGGGAGTGGCGTT+AGG - chr6.4:46160538-46160557 None:intergenic 60.0%
CGCGGACCTTCCAAGGAAGA+AGG - chr6.4:46160417-46160436 None:intergenic 60.0%
CGCGGAGGATTTGTCGGGTT+TGG + chr6.4:46161173-46161192 MS.gene062655:CDS 60.0%
CGGTTAGGGCTTGCAAAGCG+GGG + chr6.4:46160959-46160978 MS.gene062655:CDS 60.0%
GAAAAGTGAAGCCACCGCCG+TGG - chr6.4:46160696-46160715 None:intergenic 60.0%
GCCGAAGAAGTCACCGGAGA+TGG + chr6.4:46160628-46160647 MS.gene062655:CDS 60.0%
GCGGTTAGGGCTTGCAAAGC+GGG + chr6.4:46160958-46160977 MS.gene062655:CDS 60.0%
GGAAACGCCGTTAACGGGAG+TGG - chr6.4:46160545-46160564 None:intergenic 60.0%
TGCGACGGATAGCGATGCTC+TGG - chr6.4:46160811-46160830 None:intergenic 60.0%
TGTATGTGGGTCGAACGCGG+AGG + chr6.4:46161158-46161177 MS.gene062655:CDS 60.0%
TTCCTAGCCGGAGATAGCGC+CGG + chr6.4:46160913-46160932 MS.gene062655:CDS 60.0%
TTTACCGGAGGGATCGGACC+GGG + chr6.4:46161119-46161138 MS.gene062655:CDS 60.0%
! ATGGTAGCGCGGACCTTCCA+AGG - chr6.4:46160424-46160443 None:intergenic 60.0%
ATCGGACCGGGATCATGGCG+CGG + chr6.4:46161131-46161150 MS.gene062655:CDS 65.0%
CGGAGGAGAAGAACGGACGG+AGG + chr6.4:46161029-46161048 MS.gene062655:CDS 65.0%
GAGGGATCGGACCGGGATCA+TGG + chr6.4:46161126-46161145 MS.gene062655:CDS 65.0%
TGATCCCGGTCCGATCCCTC+CGG - chr6.4:46161126-46161145 None:intergenic 65.0%
CACCGGCGCTATCTCCGGCT+AGG - chr6.4:46160918-46160937 None:intergenic 70.0%
! CTAGCCGGAGATAGCGCCGG+TGG + chr6.4:46160916-46160935 MS.gene062655:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 46160391 46161366 46160391 ID=MS.gene062655
chr6.4 mRNA 46160391 46161366 46160391 ID=MS.gene062655.t1;Parent=MS.gene062655
chr6.4 exon 46160391 46161256 46160391 ID=MS.gene062655.t1.exon1;Parent=MS.gene062655.t1
chr6.4 CDS 46160391 46161256 46160391 ID=cds.MS.gene062655.t1;Parent=MS.gene062655.t1
chr6.4 exon 46161363 46161366 46161363 ID=MS.gene062655.t1.exon2;Parent=MS.gene062655.t1
chr6.4 CDS 46161363 46161366 46161363 ID=cds.MS.gene062655.t1;Parent=MS.gene062655.t1
Gene Sequence

>MS.gene062655

ATGTCTACAAACAAACCCAAACCCCTTCTTCCTTGGAAGGTCCGCGCTACCATTTCACTTCTTACAGTTTTAACAGATGCTTCATGTCGAGCTAACGGCACCGTCAACCGCCGTCTCTTTAACTTCTTCGATCTCAAATCCTCACCTAACGCCACTCCCGTTAACGGCGTTTCCACCAAAGACGTTACCGTTAACGCTGAAAATAACGTTTGGTTCCGTCTCTTTACTCCCATTGTTGCCGAAGAAGTCACCGGAGATGGTGGTTCCACCAAAACAACCTCCCTTCCTGTCATCATTTTCTTCCACGGCGGTGGCTTCACTTTTCTTTCTTCTTCCTCAAACCTCTACGATGCTGTTTGCCGTAGACTCTGCCGCAAAATCTCCGCTGTCGTCGTCTCAGTCAACTACCGACTTGCACCAGAGCATCGCTATCCGTCGCAATATGAAGACGGAGAAGAAGTTCTGAGATTTCTTGACGAGAACGTAACAGTTTTACCGGGAAATGCTGACTTATCAAAATGTTTCCTAGCCGGAGATAGCGCCGGTGGAAATTTGGCACACCATGTTGCGGTTAGGGCTTGCAAAGCGGGGCTCCAGAATATCCGGGTTATCGGGTCAATATTGATCCAACCGTTCTTCGGAGGAGAAGAACGGACGGAGGCGGAGATTCATCTAGTTGGGATGCCGTTTGTGTCAGTGGCAAGGACTGATTGGATGTGGAAAGTGTTTTTACCGGAGGGATCGGACCGGGATCATGGCGCGGTTAATGTATGTGGGTCGAACGCGGAGGATTTGTCGGGTTTGGATTATCCGGATACGCTTGTTTTTGTTGGTGGGTTTGATCCTTTGATTGATTGGCAAAAGAGGTATTATGATTGGTTGAAAAAATGTGGGAAAAAAGCTGAGTTGATTGAATATCCAAACATGGTTCATGCTTTTCATGTTTTTCCTGATTTTCCTGAATCTACTCAGTTGA
Protein sequence

>MS.gene062655.t1

MSTNKPKPLLPWKVRATISLLTVLTDASCRANGTVNRRLFNFFDLKSSPNATPVNGVSTKDVTVNAENNVWFRLFTPIVAEEVTGDGGSTKTTSLPVIIFFHGGGFTFLSSSSNLYDAVCRRLCRKISAVVVSVNYRLAPEHRYPSQYEDGEEVLRFLDENVTVLPGNADLSKCFLAGDSAGGNLAHHVAVRACKAGLQNIRVIGSILIQPFFGGEERTEAEIHLVGMPFVSVARTDWMWKVFLPEGSDRDHGAVNVCGSNAEDLSGLDYPDTLVFVGGFDPLIDWQKS