Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07155.t1 | KEH43271.1 | 98.8 | 344 | 4 | 0 | 1 | 344 | 1 | 344 | 6.10E-203 | 716.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07155.t1 | Q9LYC1 | 78.4 | 342 | 73 | 1 | 1 | 341 | 1 | 342 | 5.6e-165 | 581.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07155.t1 | A0A072VNR2 | 98.8 | 344 | 4 | 0 | 1 | 344 | 1 | 344 | 4.4e-203 | 716.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene54227 | MS.gene07155 | PPI |
| MS.gene068475 | MS.gene07155 | PPI |
| MS.gene042778 | MS.gene07155 | PPI |
| MS.gene042786 | MS.gene07155 | PPI |
| MS.gene062660 | MS.gene07155 | PPI |
| MS.gene062658 | MS.gene07155 | PPI |
| MS.gene07155 | MS.gene005188 | PPI |
| MS.gene062669 | MS.gene07155 | PPI |
| MS.gene07155 | MS.gene042787 | PPI |
| MS.gene062655 | MS.gene07155 | PPI |
| MS.gene07155 | MS.gene52621 | PPI |
| MS.gene07155 | MS.gene068472 | PPI |
| MS.gene44055 | MS.gene07155 | PPI |
| MS.gene068460 | MS.gene07155 | PPI |
| MS.gene93630 | MS.gene07155 | PPI |
| MS.gene07155 | MS.gene068477 | PPI |
| MS.gene91548 | MS.gene07155 | PPI |
| MS.gene07155 | MS.gene062670 | PPI |
| MS.gene93628 | MS.gene07155 | PPI |
| MS.gene07155 | MS.gene042779 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07155.t1 | MTR_1g089310 | 98.837 | 344 | 4 | 0 | 1 | 344 | 17 | 360 | 0.0 | 710 |
| MS.gene07155.t1 | MTR_1g089310 | 98.837 | 344 | 4 | 0 | 1 | 344 | 1 | 344 | 0.0 | 709 |
| MS.gene07155.t1 | MTR_7g093950 | 84.286 | 350 | 49 | 4 | 1 | 344 | 1 | 350 | 0.0 | 610 |
| MS.gene07155.t1 | MTR_1g082210 | 71.884 | 345 | 95 | 2 | 1 | 344 | 1 | 344 | 0.0 | 528 |
| MS.gene07155.t1 | MTR_6g065400 | 39.233 | 339 | 197 | 3 | 7 | 341 | 1 | 334 | 4.30e-80 | 248 |
| MS.gene07155.t1 | MTR_6g065110 | 40.729 | 329 | 186 | 3 | 17 | 341 | 12 | 335 | 3.64e-78 | 243 |
| MS.gene07155.t1 | MTR_6g065370 | 38.393 | 336 | 198 | 3 | 10 | 341 | 4 | 334 | 9.36e-77 | 239 |
| MS.gene07155.t1 | MTR_6g065120 | 38.507 | 335 | 198 | 4 | 10 | 340 | 5 | 335 | 2.71e-76 | 238 |
| MS.gene07155.t1 | MTR_6g065270 | 36.607 | 336 | 208 | 3 | 9 | 340 | 1 | 335 | 3.51e-73 | 230 |
| MS.gene07155.t1 | MTR_6g065430 | 36.607 | 336 | 195 | 4 | 11 | 340 | 4 | 327 | 5.64e-68 | 216 |
| MS.gene07155.t1 | MTR_6g065340 | 35.398 | 339 | 191 | 5 | 10 | 341 | 12 | 329 | 1.69e-62 | 202 |
| MS.gene07155.t1 | MTR_4g050140 | 35.034 | 294 | 158 | 6 | 61 | 341 | 43 | 316 | 2.36e-44 | 155 |
| MS.gene07155.t1 | MTR_1g012430 | 34.328 | 268 | 154 | 6 | 92 | 340 | 43 | 307 | 6.40e-41 | 145 |
| MS.gene07155.t1 | MTR_3g012440 | 36.975 | 238 | 137 | 4 | 96 | 322 | 79 | 314 | 1.19e-40 | 145 |
| MS.gene07155.t1 | MTR_7g107040 | 36.957 | 230 | 130 | 4 | 105 | 321 | 69 | 296 | 8.69e-40 | 143 |
| MS.gene07155.t1 | MTR_1g111280 | 36.885 | 244 | 133 | 6 | 98 | 323 | 81 | 321 | 1.06e-39 | 143 |
| MS.gene07155.t1 | MTR_7g406880 | 33.564 | 289 | 156 | 8 | 61 | 325 | 54 | 330 | 6.72e-39 | 141 |
| MS.gene07155.t1 | MTR_1g102170 | 40.517 | 232 | 122 | 8 | 89 | 308 | 80 | 307 | 7.93e-38 | 138 |
| MS.gene07155.t1 | MTR_8g035540 | 33.217 | 286 | 150 | 9 | 37 | 301 | 13 | 278 | 2.17e-36 | 134 |
| MS.gene07155.t1 | MTR_6g464510 | 41.477 | 176 | 94 | 4 | 97 | 264 | 74 | 248 | 3.73e-36 | 133 |
| MS.gene07155.t1 | MTR_7g056207 | 33.103 | 290 | 162 | 10 | 69 | 344 | 82 | 353 | 1.55e-35 | 132 |
| MS.gene07155.t1 | MTR_4g086480 | 32.540 | 252 | 150 | 8 | 102 | 340 | 74 | 318 | 5.01e-35 | 130 |
| MS.gene07155.t1 | MTR_4g086510 | 35.200 | 250 | 143 | 7 | 105 | 341 | 75 | 318 | 1.49e-34 | 129 |
| MS.gene07155.t1 | MTR_6g464120 | 31.788 | 302 | 173 | 10 | 32 | 321 | 23 | 303 | 2.06e-34 | 132 |
| MS.gene07155.t1 | MTR_6g022710 | 34.749 | 259 | 128 | 5 | 105 | 323 | 160 | 417 | 3.29e-34 | 130 |
| MS.gene07155.t1 | MTR_3g450680 | 32.441 | 299 | 162 | 9 | 70 | 341 | 47 | 332 | 4.84e-34 | 128 |
| MS.gene07155.t1 | MTR_0007s0520 | 37.037 | 189 | 108 | 4 | 87 | 265 | 59 | 246 | 1.92e-33 | 126 |
| MS.gene07155.t1 | MTR_6g464320 | 35.345 | 232 | 138 | 5 | 103 | 323 | 79 | 309 | 2.29e-33 | 126 |
| MS.gene07155.t1 | MTR_1g102160 | 36.979 | 192 | 109 | 3 | 84 | 264 | 57 | 247 | 5.81e-33 | 125 |
| MS.gene07155.t1 | MTR_6g464280 | 37.156 | 218 | 123 | 6 | 103 | 308 | 78 | 293 | 9.26e-33 | 124 |
| MS.gene07155.t1 | MTR_3g101420 | 29.677 | 310 | 173 | 9 | 38 | 334 | 20 | 297 | 1.96e-32 | 123 |
| MS.gene07155.t1 | MTR_4g086470 | 31.727 | 249 | 150 | 7 | 105 | 340 | 76 | 317 | 4.70e-32 | 122 |
| MS.gene07155.t1 | MTR_8g035520 | 32.636 | 239 | 135 | 7 | 100 | 321 | 74 | 303 | 1.15e-30 | 119 |
| MS.gene07155.t1 | MTR_5g005920 | 30.816 | 331 | 182 | 13 | 25 | 334 | 9 | 313 | 1.32e-30 | 119 |
| MS.gene07155.t1 | MTR_8g035500 | 30.128 | 312 | 180 | 11 | 25 | 321 | 8 | 296 | 6.75e-30 | 116 |
| MS.gene07155.t1 | MTR_8g040860 | 30.970 | 268 | 163 | 7 | 70 | 322 | 47 | 307 | 1.06e-29 | 116 |
| MS.gene07155.t1 | MTR_1g105020 | 30.169 | 295 | 168 | 8 | 38 | 314 | 21 | 295 | 9.57e-29 | 113 |
| MS.gene07155.t1 | MTR_7g051020 | 34.351 | 262 | 146 | 8 | 100 | 341 | 71 | 326 | 4.64e-28 | 112 |
| MS.gene07155.t1 | MTR_5g005900 | 30.870 | 230 | 135 | 7 | 95 | 305 | 64 | 288 | 2.85e-27 | 109 |
| MS.gene07155.t1 | MTR_4g086540 | 29.670 | 273 | 148 | 8 | 38 | 304 | 31 | 265 | 3.33e-27 | 109 |
| MS.gene07155.t1 | MTR_1g104933 | 33.588 | 262 | 148 | 7 | 98 | 341 | 64 | 317 | 2.14e-26 | 107 |
| MS.gene07155.t1 | MTR_5g005850 | 27.914 | 326 | 189 | 11 | 38 | 340 | 26 | 328 | 1.84e-25 | 104 |
| MS.gene07155.t1 | MTR_1g104930 | 32.707 | 266 | 149 | 8 | 98 | 341 | 66 | 323 | 1.07e-23 | 100 |
| MS.gene07155.t1 | MTR_0007s0480 | 38.202 | 89 | 48 | 1 | 184 | 265 | 4 | 92 | 7.34e-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07155.t1 | AT3G63010 | 78.363 | 342 | 73 | 1 | 1 | 341 | 1 | 342 | 0.0 | 579 |
| MS.gene07155.t1 | AT5G27320 | 72.464 | 345 | 92 | 3 | 1 | 344 | 1 | 343 | 0.0 | 522 |
| MS.gene07155.t1 | AT3G05120 | 69.942 | 346 | 101 | 3 | 1 | 344 | 1 | 345 | 0.0 | 509 |
| MS.gene07155.t1 | AT5G23530 | 36.000 | 350 | 190 | 7 | 1 | 340 | 1 | 326 | 9.07e-65 | 208 |
| MS.gene07155.t1 | AT5G16080 | 36.742 | 264 | 141 | 7 | 100 | 343 | 87 | 344 | 1.30e-46 | 161 |
| MS.gene07155.t1 | AT1G68620 | 34.843 | 287 | 152 | 11 | 69 | 341 | 65 | 330 | 8.09e-45 | 157 |
| MS.gene07155.t1 | AT5G06570 | 37.959 | 245 | 135 | 6 | 93 | 321 | 62 | 305 | 1.39e-37 | 137 |
| MS.gene07155.t1 | AT5G06570 | 37.959 | 245 | 135 | 6 | 93 | 321 | 62 | 305 | 1.39e-37 | 137 |
| MS.gene07155.t1 | AT5G06570 | 37.959 | 245 | 135 | 6 | 93 | 321 | 77 | 320 | 2.61e-37 | 137 |
| MS.gene07155.t1 | AT5G06570 | 37.959 | 245 | 135 | 6 | 93 | 321 | 77 | 320 | 2.61e-37 | 137 |
| MS.gene07155.t1 | AT1G47480 | 30.284 | 317 | 191 | 9 | 33 | 341 | 16 | 310 | 2.66e-36 | 134 |
| MS.gene07155.t1 | AT5G14310 | 35.094 | 265 | 124 | 7 | 105 | 323 | 151 | 413 | 1.49e-35 | 134 |
| MS.gene07155.t1 | AT2G45600 | 33.992 | 253 | 144 | 7 | 84 | 316 | 43 | 292 | 1.86e-35 | 132 |
| MS.gene07155.t1 | AT5G62180 | 32.365 | 241 | 145 | 6 | 32 | 264 | 15 | 245 | 3.32e-35 | 131 |
| MS.gene07155.t1 | AT1G19190 | 35.461 | 282 | 139 | 11 | 53 | 315 | 30 | 287 | 4.48e-35 | 130 |
| MS.gene07155.t1 | AT2G45610 | 34.940 | 249 | 145 | 7 | 105 | 340 | 79 | 323 | 3.15e-32 | 123 |
| MS.gene07155.t1 | AT3G48700 | 33.544 | 316 | 168 | 13 | 50 | 340 | 27 | 325 | 8.33e-32 | 122 |
| MS.gene07155.t1 | AT3G48690 | 33.451 | 284 | 150 | 10 | 39 | 304 | 19 | 281 | 1.51e-31 | 121 |
| MS.gene07155.t1 | AT2G03550 | 31.153 | 321 | 175 | 13 | 39 | 341 | 19 | 311 | 4.40e-31 | 119 |
| MS.gene07155.t1 | AT3G27320 | 33.962 | 265 | 126 | 8 | 105 | 323 | 134 | 395 | 3.06e-30 | 119 |
| MS.gene07155.t1 | AT3G27320 | 33.962 | 265 | 126 | 8 | 105 | 323 | 166 | 427 | 3.61e-30 | 120 |
| MS.gene07155.t1 | AT1G49650 | 31.298 | 262 | 156 | 7 | 53 | 304 | 86 | 333 | 8.08e-27 | 109 |
| MS.gene07155.t1 | AT1G49640 | 32.039 | 206 | 129 | 7 | 105 | 304 | 74 | 274 | 5.64e-26 | 106 |
| MS.gene07155.t1 | AT1G49660 | 28.947 | 266 | 169 | 7 | 49 | 303 | 21 | 277 | 3.78e-24 | 101 |
Find 95 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACATCTTGGTTAGATTTC+TGG | 0.175894 | 1.4:-71006466 | None:intergenic |
| ATGGTTGGAATGCTCTAAAT+TGG | 0.260815 | 1.4:+71006042 | MS.gene07155:CDS |
| GATCATGTCGATAGAAATTC+AGG | 0.292227 | 1.4:+71005762 | MS.gene07155:CDS |
| TTGTTACCTTAGATTCATTA+AGG | 0.302473 | 1.4:-71004868 | None:intergenic |
| CCTTGTTTGTGTGGCTGGTT+TGG | 0.311025 | 1.4:+71006403 | MS.gene07155:CDS |
| GGTAAAGAAGTTTGACATCT+TGG | 0.317354 | 1.4:-71006478 | None:intergenic |
| ATTCTTCTCCATCCACTCTT+TGG | 0.317740 | 1.4:+71006203 | MS.gene07155:CDS |
| AACCAATAGTGGCTTCCTTT+AGG | 0.329579 | 1.4:-71006499 | None:intergenic |
| ATTGGGTCAAATCAAGGATA+TGG | 0.339743 | 1.4:+71006060 | MS.gene07155:CDS |
| AAAAGCCTTGTTTGTGTGGC+TGG | 0.339972 | 1.4:+71006398 | MS.gene07155:CDS |
| ATTGTAAGCAAGCTTGAAAT+TGG | 0.347441 | 1.4:-71005637 | None:intergenic |
| CAATAGAAATGATCATTATT+AGG | 0.355983 | 1.4:-71006533 | None:intergenic |
| TACCTAAAGGAAGCCACTAT+TGG | 0.357783 | 1.4:+71006497 | MS.gene07155:CDS |
| GCCAATACAATTCCTGTAGA+TGG | 0.359532 | 1.4:+71005726 | MS.gene07155:CDS |
| AGTCAACCTTAATGAATCTA+AGG | 0.367754 | 1.4:+71004862 | MS.gene07155:CDS |
| TGATCTCTATCTTCCCCTTC+AGG | 0.371490 | 1.4:-71006317 | None:intergenic |
| GTTTGGATCTTCTACAAGAT+TGG | 0.373570 | 1.4:+71006420 | MS.gene07155:CDS |
| TTCTCCATCCACTCTTTGGT+GGG | 0.385254 | 1.4:+71006207 | MS.gene07155:CDS |
| TGCAAGATCGTGATTGGTAT+TGG | 0.387188 | 1.4:+71006282 | MS.gene07155:CDS |
| AACAGAATCGGAGAAGAAAC+TGG | 0.395775 | 1.4:+71006238 | MS.gene07155:CDS |
| CCTAAAGGTAAAAGCCTTGT+AGG | 0.397562 | 1.4:+71006362 | MS.gene07155:CDS |
| CTCCAAGGTTTATGCTTACA+TGG | 0.407235 | 1.4:+71006100 | MS.gene07155:CDS |
| CTCCAGAGAATGTTACTACT+TGG | 0.411618 | 1.4:+71005811 | MS.gene07155:CDS |
| CTTCTCCATCCACTCTTTGG+TGG | 0.413791 | 1.4:+71006206 | MS.gene07155:CDS |
| GTGTTGTTCCTCTCAATACT+TGG | 0.417333 | 1.4:+71005604 | MS.gene07155:CDS |
| CATCCTGCTTGTAATCCATT+TGG | 0.419839 | 1.4:+71006338 | MS.gene07155:CDS |
| CCCCAAGTAGTAACATTCTC+TGG | 0.421392 | 1.4:-71005813 | None:intergenic |
| TGTAATCCATTTGGTCCTAA+AGG | 0.430276 | 1.4:+71006347 | MS.gene07155:CDS |
| CTCTAAATTGGGTCAAATCA+AGG | 0.442412 | 1.4:+71006054 | MS.gene07155:CDS |
| AAGGTTTATGCTTACATGGC+TGG | 0.447846 | 1.4:+71006104 | MS.gene07155:CDS |
| TATCCGTGTGCTTACGAAGA+TGG | 0.455575 | 1.4:+71006023 | MS.gene07155:CDS |
| TACATGGCTGGTGATAGTTC+CGG | 0.457192 | 1.4:+71006116 | MS.gene07155:CDS |
| TGTCAAACTTCTTTACCTAA+AGG | 0.457617 | 1.4:+71006484 | MS.gene07155:CDS |
| CCCTAAAAGCCTTGTTTGTG+TGG | 0.460173 | 1.4:+71006394 | MS.gene07155:CDS |
| TTGTTGTTTCTGTAAACTAC+AGG | 0.460275 | 1.4:+71005982 | MS.gene07155:CDS |
| TCTTCTACAAGATTGGCAAT+TGG | 0.460325 | 1.4:+71006427 | MS.gene07155:CDS |
| GACAATCTCTGTTGTGCTCA+AGG | 0.461484 | 1.4:-71005856 | None:intergenic |
| TCTACAGGAATTGTATTGGC+TGG | 0.475234 | 1.4:-71005723 | None:intergenic |
| TGAGGTTGCAAGATCGTGAT+TGG | 0.487767 | 1.4:+71006276 | MS.gene07155:CDS |
| TCCATCTACAGGAATTGTAT+TGG | 0.489416 | 1.4:-71005727 | None:intergenic |
| TCTCCATCCACTCTTTGGTG+GGG | 0.492850 | 1.4:+71006208 | MS.gene07155:CDS |
| GAGGAAGATGTTGAGGTACT+AGG | 0.496473 | 1.4:+71006176 | MS.gene07155:CDS |
| AAAGAGAATACTCCATCTAC+AGG | 0.500048 | 1.4:-71005738 | None:intergenic |
| CTCTCTATTGAATGTTCCAT+CGG | 0.504062 | 1.4:-71005673 | None:intergenic |
| AACATAGACATAAACATGGC+TGG | 0.504836 | 1.4:+71004830 | None:intergenic |
| TGGTTGGAATGCTCTAAATT+GGG | 0.506865 | 1.4:+71006043 | MS.gene07155:CDS |
| GTAGTAACATTCTCTGGAGC+AGG | 0.509928 | 1.4:-71005807 | None:intergenic |
| TCGCCTTGTGAGCATGTGTA+AGG | 0.514102 | 1.4:+71005956 | MS.gene07155:CDS |
| TCCAGAGAATGTTACTACTT+GGG | 0.521238 | 1.4:+71005812 | MS.gene07155:CDS |
| TAGGGAACTTGAGTCCTACA+AGG | 0.521873 | 1.4:-71006376 | None:intergenic |
| TGGGGAGAAGCGAACAGAAT+CGG | 0.526899 | 1.4:+71006226 | MS.gene07155:CDS |
| GGACCAAATGGATTACAAGC+AGG | 0.530885 | 1.4:-71006341 | None:intergenic |
| AGATTGGCAATTGGAATATG+TGG | 0.530969 | 1.4:+71006436 | MS.gene07155:CDS |
| CCTGTCATAATCTTCTTCCA+TGG | 0.533405 | 1.4:+71005879 | MS.gene07155:CDS |
| CTACAGGAATTGTATTGGCT+GGG | 0.533816 | 1.4:-71005722 | None:intergenic |
| TGGAAGTTTCTCTCATTCCT+CGG | 0.536571 | 1.4:+71005902 | MS.gene07155:CDS |
| TGTTGTTCCTCTCAATACTT+GGG | 0.536632 | 1.4:+71005605 | MS.gene07155:CDS |
| GTAGCAGTATATCAGCAGTT+AGG | 0.539039 | 1.4:-71006587 | None:intergenic |
| CGTGTGCTTACGAAGATGGT+TGG | 0.544969 | 1.4:+71006027 | MS.gene07155:CDS |
| AGAAATGATCATTATTAGGA+AGG | 0.548626 | 1.4:-71006529 | None:intergenic |
| CCAGAGAATGTTACTACTTG+GGG | 0.553071 | 1.4:+71005813 | MS.gene07155:CDS |
| CAGCCTTACACATGCTCACA+AGG | 0.557141 | 1.4:-71005959 | None:intergenic |
| CCATGGAAGAAGATTATGAC+AGG | 0.559426 | 1.4:-71005879 | None:intergenic |
| GCACACGGATACCGATGCTC+GGG | 0.559770 | 1.4:-71006011 | None:intergenic |
| CGTTGCGGTGAGAGCTGCCG+AGG | 0.561498 | 1.4:+71006157 | MS.gene07155:CDS |
| GTGATGAGCAATGTTGCCTC+CGG | 0.563388 | 1.4:-71006135 | None:intergenic |
| ACAATCTCTGTTGTGCTCAA+GGG | 0.564360 | 1.4:-71005855 | None:intergenic |
| AGCTGAGTTTCTTGACCGCA+AGG | 0.568671 | 1.4:+71005698 | MS.gene07155:CDS |
| AGCACACGGATACCGATGCT+CGG | 0.568740 | 1.4:-71006012 | None:intergenic |
| TAGTACCTCAACATCTTCCT+CGG | 0.571640 | 1.4:-71006174 | None:intergenic |
| AAGGAAGTAGAAACCAATAG+TGG | 0.572842 | 1.4:-71006510 | None:intergenic |
| TCTCCCCACCAAAGAGTGGA+TGG | 0.573660 | 1.4:-71006211 | None:intergenic |
| AGCTGCCGAGGAAGATGTTG+AGG | 0.574921 | 1.4:+71006169 | MS.gene07155:CDS |
| AATCTACTAAGACGCGCCGA+TGG | 0.574971 | 1.4:+71005657 | MS.gene07155:CDS |
| GAATCGGAGAAGAAACTGGA+TGG | 0.579411 | 1.4:+71006242 | MS.gene07155:CDS |
| CGCTTCTCCCCACCAAAGAG+TGG | 0.583817 | 1.4:-71006215 | None:intergenic |
| TTGTATTGGCTGGGACCTTG+CGG | 0.585043 | 1.4:-71005713 | None:intergenic |
| ACAGGAGATCACCCGAGCAT+CGG | 0.608623 | 1.4:+71006000 | MS.gene07155:CDS |
| CCAAACCAGCCACACAAACA+AGG | 0.618481 | 1.4:-71006403 | None:intergenic |
| TCAAAGTGGAAAGGACTCCA+AGG | 0.619865 | 1.4:+71006085 | MS.gene07155:CDS |
| AGCCATGTAAGCATAAACCT+TGG | 0.620826 | 1.4:-71006102 | None:intergenic |
| TGGCAATTGGAATATGTGGA+AGG | 0.626681 | 1.4:+71006440 | MS.gene07155:CDS |
| GTCATAATCTTCTTCCATGG+TGG | 0.626745 | 1.4:+71005882 | MS.gene07155:CDS |
| AGGTGTTTATCTCATTCATG+AGG | 0.628766 | 1.4:-71006556 | None:intergenic |
| TCAAGGATATGGCTTCAAAG+TGG | 0.630695 | 1.4:+71006071 | MS.gene07155:CDS |
| CAACCATCTTCGTAAGCACA+CGG | 0.632894 | 1.4:-71006026 | None:intergenic |
| GATATGGCTTCAAAGTGGAA+AGG | 0.639590 | 1.4:+71006076 | MS.gene07155:CDS |
| ATAGATAGCACTATTAGCCG+AGG | 0.651389 | 1.4:-71005919 | None:intergenic |
| ATGGCTGGTGATAGTTCCGG+AGG | 0.657964 | 1.4:+71006119 | MS.gene07155:CDS |
| TACACATGCTCACAAGGCGA+CGG | 0.659131 | 1.4:-71005953 | None:intergenic |
| CAACATTGCTCATCACGTTG+CGG | 0.669625 | 1.4:+71006142 | MS.gene07155:CDS |
| ATAAGCACCCAAGTATTGAG+AGG | 0.671972 | 1.4:-71005612 | None:intergenic |
| AATGAGAGAAACTTCCACCA+TGG | 0.679061 | 1.4:-71005896 | None:intergenic |
| CATGGAAGAAGATTATGACA+GGG | 0.698543 | 1.4:-71005878 | None:intergenic |
| TCAGCAGTTAGGATGCACAA+AGG | 0.736695 | 1.4:-71006576 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCTAAAAACAAATAAAAGA+AGG | - | chr1.4:71004963-71004982 | None:intergenic | 15.0% |
| !! | CAATAGAAATGATCATTATT+AGG | - | chr1.4:71006536-71006555 | None:intergenic | 20.0% |
| !!! | ACAACTTTCTTTGTTTTTTT+TGG | + | chr1.4:71004887-71004906 | MS.gene07155:intron | 20.0% |
| !!! | ATAATTTCTTGCTTTTCTAA+AGG | + | chr1.4:71005360-71005379 | MS.gene07155:intron | 20.0% |
| !!! | TAATTTCTTGCTTTTCTAAA+GGG | + | chr1.4:71005361-71005380 | MS.gene07155:intron | 20.0% |
| !!! | TTCTATTTTTTCTTACATGT+AGG | + | chr1.4:71005199-71005218 | MS.gene07155:intron | 20.0% |
| !!! | TTTTTTGTGTTTTTGATGTT+GGG | + | chr1.4:71004989-71005008 | MS.gene07155:intron | 20.0% |
| !!! | TTTTTTTGTGTTTTTGATGT+TGG | + | chr1.4:71004988-71005007 | MS.gene07155:intron | 20.0% |
| ! | AATGAAGTGCATAATTCATA+CGG | + | chr1.4:71005441-71005460 | MS.gene07155:intron | 25.0% |
| ! | AGAAATGATCATTATTAGGA+AGG | - | chr1.4:71006532-71006551 | None:intergenic | 25.0% |
| ! | GCAAGAAATTATTGAAAAGA+AGG | - | chr1.4:71005352-71005371 | None:intergenic | 25.0% |
| ! | TGAAAAGAAGGATCATAAAA+AGG | - | chr1.4:71005340-71005359 | None:intergenic | 25.0% |
| ! | TTGTTACCTTAGATTCATTA+AGG | - | chr1.4:71004871-71004890 | None:intergenic | 25.0% |
| ! | TTTGAATCTTTAACATCGAT+TGG | + | chr1.4:71005034-71005053 | MS.gene07155:intron | 25.0% |
| !! | AAATTCAGGACTTTTCAATA+GGG | + | chr1.4:71005776-71005795 | MS.gene07155:CDS | 25.0% |
| !! | TTGAATCTTTAACATCGATT+GGG | + | chr1.4:71005035-71005054 | MS.gene07155:intron | 25.0% |
| !!! | TGTTTTTGTTATCCTTTTCT+GGG | + | chr1.4:71005112-71005131 | MS.gene07155:intron | 25.0% |
| !!! | TTGTTTTTGTTATCCTTTTC+TGG | + | chr1.4:71005111-71005130 | MS.gene07155:intron | 25.0% |
| AGTCAACCTTAATGAATCTA+AGG | + | chr1.4:71004862-71004881 | MS.gene07155:CDS | 30.0% | |
| ATTGTAAGCAAGCTTGAAAT+TGG | - | chr1.4:71005640-71005659 | None:intergenic | 30.0% | |
| CATCAACTATGCATTTCATT+TGG | - | chr1.4:71005546-71005565 | None:intergenic | 30.0% | |
| TGTCAAACTTCTTTACCTAA+AGG | + | chr1.4:71006484-71006503 | MS.gene07155:CDS | 30.0% | |
| TTATCAACAAAACCCAGAAA+AGG | - | chr1.4:71005127-71005146 | None:intergenic | 30.0% | |
| ! | AATGAAATGCATAGTTGATG+TGG | + | chr1.4:71005546-71005565 | MS.gene07155:intron | 30.0% |
| ! | GAAATTCAGGACTTTTCAAT+AGG | + | chr1.4:71005775-71005794 | MS.gene07155:CDS | 30.0% |
| ! | GGATCATAAAAAGGAAGTAT+AGG | - | chr1.4:71005331-71005350 | None:intergenic | 30.0% |
| !! | TTGTTGTTTCTGTAAACTAC+AGG | + | chr1.4:71005982-71006001 | MS.gene07155:CDS | 30.0% |
| AAAGAGAATACTCCATCTAC+AGG | - | chr1.4:71005741-71005760 | None:intergenic | 35.0% | |
| AAGGAAGTAGAAACCAATAG+TGG | - | chr1.4:71006513-71006532 | None:intergenic | 35.0% | |
| ACAAACCCCAAAAACTACAT+AGG | - | chr1.4:71005158-71005177 | None:intergenic | 35.0% | |
| ACCTTTCTCAAAGATTTGTG+AGG | + | chr1.4:71005295-71005314 | MS.gene07155:intron | 35.0% | |
| AGATTGGCAATTGGAATATG+TGG | + | chr1.4:71006436-71006455 | MS.gene07155:CDS | 35.0% | |
| AGGTGTTTATCTCATTCATG+AGG | - | chr1.4:71006559-71006578 | None:intergenic | 35.0% | |
| ATTGGGTCAAATCAAGGATA+TGG | + | chr1.4:71006060-71006079 | MS.gene07155:CDS | 35.0% | |
| CAAACCCCAAAAACTACATA+GGG | - | chr1.4:71005157-71005176 | None:intergenic | 35.0% | |
| CATGGAAGAAGATTATGACA+GGG | - | chr1.4:71005881-71005900 | None:intergenic | 35.0% | |
| CTCTAAATTGGGTCAAATCA+AGG | + | chr1.4:71006054-71006073 | MS.gene07155:CDS | 35.0% | |
| CTCTCTATTGAATGTTCCAT+CGG | - | chr1.4:71005676-71005695 | None:intergenic | 35.0% | |
| GATCATGTCGATAGAAATTC+AGG | + | chr1.4:71005762-71005781 | MS.gene07155:CDS | 35.0% | |
| GGAAGGATTGCATATTTGTT+GGG | + | chr1.4:71005056-71005075 | MS.gene07155:intron | 35.0% | |
| GGTAAAGAAGTTTGACATCT+TGG | - | chr1.4:71006481-71006500 | None:intergenic | 35.0% | |
| GTGCATAATTCATACGGATA+AGG | + | chr1.4:71005447-71005466 | MS.gene07155:intron | 35.0% | |
| GTTTACTTGTTCTGGTAAGT+TGG | + | chr1.4:71005229-71005248 | MS.gene07155:intron | 35.0% | |
| GTTTGGATCTTCTACAAGAT+TGG | + | chr1.4:71006420-71006439 | MS.gene07155:CDS | 35.0% | |
| TCCAGAGAATGTTACTACTT+GGG | + | chr1.4:71005812-71005831 | MS.gene07155:CDS | 35.0% | |
| TCCATCTACAGGAATTGTAT+TGG | - | chr1.4:71005730-71005749 | None:intergenic | 35.0% | |
| TCTTCTACAAGATTGGCAAT+TGG | + | chr1.4:71006427-71006446 | MS.gene07155:CDS | 35.0% | |
| TGACATCTTGGTTAGATTTC+TGG | - | chr1.4:71006469-71006488 | None:intergenic | 35.0% | |
| TGTAATCCATTTGGTCCTAA+AGG | + | chr1.4:71006347-71006366 | MS.gene07155:CDS | 35.0% | |
| TGTTGTTCCTCTCAATACTT+GGG | + | chr1.4:71005605-71005624 | MS.gene07155:CDS | 35.0% | |
| ! | ACCTCACAAATCTTTGAGAA+AGG | - | chr1.4:71005299-71005318 | None:intergenic | 35.0% |
| ! | ATCTTTAACATCGATTGGGA+AGG | + | chr1.4:71005039-71005058 | MS.gene07155:intron | 35.0% |
| ! | ATGGTCCCTATGTAGTTTTT+GGG | + | chr1.4:71005149-71005168 | MS.gene07155:intron | 35.0% |
| ! | ATGGTTGGAATGCTCTAAAT+TGG | + | chr1.4:71006042-71006061 | MS.gene07155:CDS | 35.0% |
| ! | CACAAATCTTTGAGAAAGGT+GGG | - | chr1.4:71005295-71005314 | None:intergenic | 35.0% |
| ! | TCACAAATCTTTGAGAAAGG+TGG | - | chr1.4:71005296-71005315 | None:intergenic | 35.0% |
| ! | TGGTTGGAATGCTCTAAATT+GGG | + | chr1.4:71006043-71006062 | MS.gene07155:CDS | 35.0% |
| !! | GTGTTTTTGATGTTGGGTTT+TGG | + | chr1.4:71004995-71005014 | MS.gene07155:intron | 35.0% |
| !!! | CTGGGTTTTGTTGATAATGA+TGG | + | chr1.4:71005130-71005149 | MS.gene07155:intron | 35.0% |
| !!! | CTTTTACCTTTAGGACCAAA+TGG | - | chr1.4:71006356-71006375 | None:intergenic | 35.0% |
| !!! | TGGATGGAAAGTATTTTGTG+AGG | + | chr1.4:71006258-71006277 | MS.gene07155:CDS | 35.0% |
| AACAGAATCGGAGAAGAAAC+TGG | + | chr1.4:71006238-71006257 | MS.gene07155:CDS | 40.0% | |
| AACCAATAGTGGCTTCCTTT+AGG | - | chr1.4:71006502-71006521 | None:intergenic | 40.0% | |
| AAGGTTTATGCTTACATGGC+TGG | + | chr1.4:71006104-71006123 | MS.gene07155:CDS | 40.0% | |
| AATGAGAGAAACTTCCACCA+TGG | - | chr1.4:71005899-71005918 | None:intergenic | 40.0% | |
| ACAATCTCTGTTGTGCTCAA+GGG | - | chr1.4:71005858-71005877 | None:intergenic | 40.0% | |
| ACCAGAACAAGTAAACCAAG+AGG | - | chr1.4:71005225-71005244 | None:intergenic | 40.0% | |
| AGCCATGTAAGCATAAACCT+TGG | - | chr1.4:71006105-71006124 | None:intergenic | 40.0% | |
| ATAGATAGCACTATTAGCCG+AGG | - | chr1.4:71005922-71005941 | None:intergenic | 40.0% | |
| ATTCTTCTCCATCCACTCTT+TGG | + | chr1.4:71006203-71006222 | MS.gene07155:CDS | 40.0% | |
| CATCCTGCTTGTAATCCATT+TGG | + | chr1.4:71006338-71006357 | MS.gene07155:CDS | 40.0% | |
| CCAGAGAATGTTACTACTTG+GGG | + | chr1.4:71005813-71005832 | MS.gene07155:CDS | 40.0% | |
| CCATGGAAGAAGATTATGAC+AGG | - | chr1.4:71005882-71005901 | None:intergenic | 40.0% | |
| CCTGTCATAATCTTCTTCCA+TGG | + | chr1.4:71005879-71005898 | MS.gene07155:CDS | 40.0% | |
| CTCCAAGGTTTATGCTTACA+TGG | + | chr1.4:71006100-71006119 | MS.gene07155:CDS | 40.0% | |
| CTCCAGAGAATGTTACTACT+TGG | + | chr1.4:71005811-71005830 | MS.gene07155:CDS | 40.0% | |
| GATATGGCTTCAAAGTGGAA+AGG | + | chr1.4:71006076-71006095 | MS.gene07155:CDS | 40.0% | |
| GCCAATACAATTCCTGTAGA+TGG | + | chr1.4:71005726-71005745 | MS.gene07155:CDS | 40.0% | |
| GGGAAGGATTGCATATTTGT+TGG | + | chr1.4:71005055-71005074 | MS.gene07155:intron | 40.0% | |
| GTCATAATCTTCTTCCATGG+TGG | + | chr1.4:71005882-71005901 | MS.gene07155:CDS | 40.0% | |
| TACCTAAAGGAAGCCACTAT+TGG | + | chr1.4:71006497-71006516 | MS.gene07155:CDS | 40.0% | |
| TAGTACCTCAACATCTTCCT+CGG | - | chr1.4:71006177-71006196 | None:intergenic | 40.0% | |
| TCAAGGATATGGCTTCAAAG+TGG | + | chr1.4:71006071-71006090 | MS.gene07155:CDS | 40.0% | |
| TGCAAGATCGTGATTGGTAT+TGG | + | chr1.4:71006282-71006301 | MS.gene07155:CDS | 40.0% | |
| TGGAAGTTTCTCTCATTCCT+CGG | + | chr1.4:71005902-71005921 | MS.gene07155:CDS | 40.0% | |
| TGGCAATTGGAATATGTGGA+AGG | + | chr1.4:71006440-71006459 | MS.gene07155:CDS | 40.0% | |
| TTTCTTACATGTAGGCCTCT+TGG | + | chr1.4:71005207-71005226 | MS.gene07155:intron | 40.0% | |
| ! | AATGCATAGTTGATGTGGAC+AGG | + | chr1.4:71005551-71005570 | MS.gene07155:intron | 40.0% |
| ! | ATGCATAGTTGATGTGGACA+GGG | + | chr1.4:71005552-71005571 | MS.gene07155:intron | 40.0% |
| ! | CCACACAAACAAGGCTTTTA+GGG | - | chr1.4:71006397-71006416 | None:intergenic | 40.0% |
| ! | CCTAAAGGTAAAAGCCTTGT+AGG | + | chr1.4:71006362-71006381 | MS.gene07155:CDS | 40.0% |
| ! | CCTACAAGGCTTTTACCTTT+AGG | - | chr1.4:71006365-71006384 | None:intergenic | 40.0% |
| ! | GATGGTCCCTATGTAGTTTT+TGG | + | chr1.4:71005148-71005167 | MS.gene07155:intron | 40.0% |
| ! | GTGTTGTTCCTCTCAATACT+TGG | + | chr1.4:71005604-71005623 | MS.gene07155:CDS | 40.0% |
| ! | TCTACAGGAATTGTATTGGC+TGG | - | chr1.4:71005726-71005745 | None:intergenic | 40.0% |
| ! | TGGTCCCTATGTAGTTTTTG+GGG | + | chr1.4:71005150-71005169 | MS.gene07155:intron | 40.0% |
| !! | ATAAGCACCCAAGTATTGAG+AGG | - | chr1.4:71005615-71005634 | None:intergenic | 40.0% |
| !! | CTACAGGAATTGTATTGGCT+GGG | - | chr1.4:71005725-71005744 | None:intergenic | 40.0% |
| !! | GGTGGGCAATTTTTCAACTT+TGG | - | chr1.4:71005278-71005297 | None:intergenic | 40.0% |
| !!! | GTTGGGTTTTGGTGTTGAAA+GGG | + | chr1.4:71005006-71005025 | MS.gene07155:intron | 40.0% |
| !!! | TGTTGGGTTTTGGTGTTGAA+AGG | + | chr1.4:71005005-71005024 | MS.gene07155:intron | 40.0% |
| CAACATTGCTCATCACGTTG+CGG | + | chr1.4:71006142-71006161 | MS.gene07155:CDS | 45.0% | |
| CAACCATCTTCGTAAGCACA+CGG | - | chr1.4:71006029-71006048 | None:intergenic | 45.0% | |
| CCCCAAGTAGTAACATTCTC+TGG | - | chr1.4:71005816-71005835 | None:intergenic | 45.0% | |
| GAATCGGAGAAGAAACTGGA+TGG | + | chr1.4:71006242-71006261 | MS.gene07155:CDS | 45.0% | |
| GACAATCTCTGTTGTGCTCA+AGG | - | chr1.4:71005859-71005878 | None:intergenic | 45.0% | |
| GCCTCTTGGTTTACTTGTTC+TGG | + | chr1.4:71005221-71005240 | MS.gene07155:intron | 45.0% | |
| TACATGGCTGGTGATAGTTC+CGG | + | chr1.4:71006116-71006135 | MS.gene07155:CDS | 45.0% | |
| TAGGGAACTTGAGTCCTACA+AGG | - | chr1.4:71006379-71006398 | None:intergenic | 45.0% | |
| TATCCGTGTGCTTACGAAGA+TGG | + | chr1.4:71006023-71006042 | MS.gene07155:CDS | 45.0% | |
| TCAAAGTGGAAAGGACTCCA+AGG | + | chr1.4:71006085-71006104 | MS.gene07155:CDS | 45.0% | |
| TCAGCAGTTAGGATGCACAA+AGG | - | chr1.4:71006579-71006598 | None:intergenic | 45.0% | |
| TGAGGTTGCAAGATCGTGAT+TGG | + | chr1.4:71006276-71006295 | MS.gene07155:CDS | 45.0% | |
| TGATCTCTATCTTCCCCTTC+AGG | - | chr1.4:71006320-71006339 | None:intergenic | 45.0% | |
| TTCTCCATCCACTCTTTGGT+GGG | + | chr1.4:71006207-71006226 | MS.gene07155:CDS | 45.0% | |
| ! | GAGGAAGATGTTGAGGTACT+AGG | + | chr1.4:71006176-71006195 | MS.gene07155:CDS | 45.0% |
| ! | GCCACACAAACAAGGCTTTT+AGG | - | chr1.4:71006398-71006417 | None:intergenic | 45.0% |
| !! | AAAAGCCTTGTTTGTGTGGC+TGG | + | chr1.4:71006398-71006417 | MS.gene07155:CDS | 45.0% |
| !! | CCCTAAAAGCCTTGTTTGTG+TGG | + | chr1.4:71006394-71006413 | MS.gene07155:CDS | 45.0% |
| !! | GGACCAAATGGATTACAAGC+AGG | - | chr1.4:71006344-71006363 | None:intergenic | 45.0% |
| !! | GTAGTAACATTCTCTGGAGC+AGG | - | chr1.4:71005810-71005829 | None:intergenic | 45.0% |
| AATCTACTAAGACGCGCCGA+TGG | + | chr1.4:71005657-71005676 | MS.gene07155:CDS | 50.0% | |
| AGCTGAGTTTCTTGACCGCA+AGG | + | chr1.4:71005698-71005717 | MS.gene07155:CDS | 50.0% | |
| CAGCCTTACACATGCTCACA+AGG | - | chr1.4:71005962-71005981 | None:intergenic | 50.0% | |
| CCAAACCAGCCACACAAACA+AGG | - | chr1.4:71006406-71006425 | None:intergenic | 50.0% | |
| CGTGTGCTTACGAAGATGGT+TGG | + | chr1.4:71006027-71006046 | MS.gene07155:CDS | 50.0% | |
| CTTCTCCATCCACTCTTTGG+TGG | + | chr1.4:71006206-71006225 | MS.gene07155:CDS | 50.0% | |
| GTGATGAGCAATGTTGCCTC+CGG | - | chr1.4:71006138-71006157 | None:intergenic | 50.0% | |
| TACACATGCTCACAAGGCGA+CGG | - | chr1.4:71005956-71005975 | None:intergenic | 50.0% | |
| TCGCCTTGTGAGCATGTGTA+AGG | + | chr1.4:71005956-71005975 | MS.gene07155:CDS | 50.0% | |
| TCTCCATCCACTCTTTGGTG+GGG | + | chr1.4:71006208-71006227 | MS.gene07155:CDS | 50.0% | |
| TGGGGAGAAGCGAACAGAAT+CGG | + | chr1.4:71006226-71006245 | MS.gene07155:CDS | 50.0% | |
| ! | CCTTGTTTGTGTGGCTGGTT+TGG | + | chr1.4:71006403-71006422 | MS.gene07155:CDS | 50.0% |
| ! | GCGAGCTTTTCTACCTGAAG+GGG | + | chr1.4:71006304-71006323 | MS.gene07155:CDS | 50.0% |
| ! | GGCGAGCTTTTCTACCTGAA+GGG | + | chr1.4:71006303-71006322 | MS.gene07155:CDS | 50.0% |
| ! | TGGCGAGCTTTTCTACCTGA+AGG | + | chr1.4:71006302-71006321 | MS.gene07155:CDS | 50.0% |
| !! | TTGTATTGGCTGGGACCTTG+CGG | - | chr1.4:71005716-71005735 | None:intergenic | 50.0% |
| ACAGGAGATCACCCGAGCAT+CGG | + | chr1.4:71006000-71006019 | MS.gene07155:CDS | 55.0% | |
| AGCACACGGATACCGATGCT+CGG | - | chr1.4:71006015-71006034 | None:intergenic | 55.0% | |
| AGCTGCCGAGGAAGATGTTG+AGG | + | chr1.4:71006169-71006188 | MS.gene07155:CDS | 55.0% | |
| ATGGCTGGTGATAGTTCCGG+AGG | + | chr1.4:71006119-71006138 | MS.gene07155:CDS | 55.0% | |
| TCTCCCCACCAAAGAGTGGA+TGG | - | chr1.4:71006214-71006233 | None:intergenic | 55.0% | |
| CGCTTCTCCCCACCAAAGAG+TGG | - | chr1.4:71006218-71006237 | None:intergenic | 60.0% | |
| GCACACGGATACCGATGCTC+GGG | - | chr1.4:71006014-71006033 | None:intergenic | 60.0% | |
| CGTTGCGGTGAGAGCTGCCG+AGG | + | chr1.4:71006157-71006176 | MS.gene07155:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 71004845 | 71006598 | 71004845 | ID=MS.gene07155 |
| chr1.4 | mRNA | 71004845 | 71006598 | 71004845 | ID=MS.gene07155.t1;Parent=MS.gene07155 |
| chr1.4 | exon | 71004845 | 71004883 | 71004845 | ID=MS.gene07155.t1.exon1;Parent=MS.gene07155.t1 |
| chr1.4 | CDS | 71004845 | 71004883 | 71004845 | ID=cds.MS.gene07155.t1;Parent=MS.gene07155.t1 |
| chr1.4 | exon | 71005603 | 71006598 | 71005603 | ID=MS.gene07155.t1.exon2;Parent=MS.gene07155.t1 |
| chr1.4 | CDS | 71005603 | 71006598 | 71005603 | ID=cds.MS.gene07155.t1;Parent=MS.gene07155.t1 |
| Gene Sequence |
| Protein sequence |
