AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04666

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04666.t1 MTR_7g093950 99.714 350 1 0 1 350 1 350 0.0 725
MS.gene04666.t1 MTR_1g089310 84.571 350 48 4 1 350 17 360 0.0 613
MS.gene04666.t1 MTR_1g089310 84.571 350 48 4 1 350 1 344 0.0 613
MS.gene04666.t1 MTR_1g082210 70.655 351 95 6 1 350 1 344 0.0 512
MS.gene04666.t1 MTR_6g065110 40.964 332 187 5 17 347 12 335 3.77e-80 248
MS.gene04666.t1 MTR_6g065400 38.889 342 200 4 7 347 1 334 4.50e-80 248
MS.gene04666.t1 MTR_6g065120 40.764 314 178 4 35 347 30 336 2.66e-79 246
MS.gene04666.t1 MTR_6g065370 37.463 339 203 4 10 347 4 334 1.86e-74 234
MS.gene04666.t1 MTR_6g065270 35.588 340 214 4 9 347 1 336 6.41e-73 230
MS.gene04666.t1 MTR_6g065430 35.988 339 199 5 11 346 4 327 9.62e-64 206
MS.gene04666.t1 MTR_6g065340 36.943 314 171 6 36 347 41 329 7.80e-63 204
MS.gene04666.t1 MTR_4g050140 35.135 296 159 8 61 346 43 315 5.76e-43 151
MS.gene04666.t1 MTR_7g406880 33.013 312 169 11 61 347 54 350 1.13e-38 141
MS.gene04666.t1 MTR_7g107040 35.776 232 135 6 106 327 69 296 3.79e-38 139
MS.gene04666.t1 MTR_1g111280 31.847 314 172 9 53 346 51 342 7.45e-37 136
MS.gene04666.t1 MTR_1g012430 31.250 304 180 9 63 346 13 307 4.14e-36 133
MS.gene04666.t1 MTR_1g102170 37.143 210 111 5 78 283 83 275 5.11e-36 134
MS.gene04666.t1 MTR_8g035540 32.692 260 156 5 99 346 64 316 2.50e-35 131
MS.gene04666.t1 MTR_3g012440 34.583 240 145 5 97 328 79 314 7.76e-35 130
MS.gene04666.t1 MTR_7g056207 34.749 259 143 8 106 350 107 353 9.59e-35 130
MS.gene04666.t1 MTR_6g464510 31.818 242 145 5 32 268 22 248 5.42e-34 128
MS.gene04666.t1 MTR_4g086510 31.699 306 178 10 53 347 33 318 1.35e-33 127
MS.gene04666.t1 MTR_0007s0520 34.109 258 156 8 100 347 72 325 4.46e-33 125
MS.gene04666.t1 MTR_6g464320 31.835 267 165 6 69 329 54 309 5.53e-33 125
MS.gene04666.t1 MTR_6g022710 30.662 287 147 5 80 329 146 417 1.67e-32 126
MS.gene04666.t1 MTR_6g464280 33.871 248 137 6 78 314 62 293 3.24e-32 123
MS.gene04666.t1 MTR_4g086480 30.116 259 152 8 103 346 74 318 4.42e-32 122
MS.gene04666.t1 MTR_6g464120 32.377 244 139 7 32 268 23 247 5.23e-32 125
MS.gene04666.t1 MTR_3g450680 31.208 298 171 12 70 346 47 331 1.09e-31 122
MS.gene04666.t1 MTR_1g102160 37.427 171 98 3 106 268 78 247 5.24e-30 117
MS.gene04666.t1 MTR_8g035500 28.981 314 187 10 25 327 8 296 5.60e-30 117
MS.gene04666.t1 MTR_4g086470 33.486 218 119 6 106 311 76 279 7.07e-30 117
MS.gene04666.t1 MTR_8g035520 29.457 258 165 5 101 347 74 325 7.10e-30 117
MS.gene04666.t1 MTR_3g101420 30.769 312 171 12 53 347 32 315 2.78e-29 115
MS.gene04666.t1 MTR_8g040860 28.228 333 197 14 32 347 22 329 3.87e-27 109
MS.gene04666.t1 MTR_5g005920 29.042 334 190 15 25 340 9 313 4.55e-26 106
MS.gene04666.t1 MTR_4g086540 29.949 197 116 5 38 231 31 208 7.13e-26 105
MS.gene04666.t1 MTR_1g105020 28.571 301 171 11 38 320 21 295 3.37e-24 101
MS.gene04666.t1 MTR_7g051020 30.743 296 166 11 69 347 53 326 5.18e-24 100
MS.gene04666.t1 MTR_5g005900 27.451 306 170 14 23 308 12 285 3.74e-23 98.6
MS.gene04666.t1 MTR_5g005850 27.356 329 193 13 38 346 26 328 1.45e-22 97.1
MS.gene04666.t1 MTR_1g104933 31.641 256 134 10 69 311 51 278 8.51e-21 91.7
MS.gene04666.t1 MTR_1g104930 31.154 260 134 11 69 311 53 284 2.04e-20 90.9
MS.gene04666.t1 MTR_0007s0480 32.941 170 103 6 186 347 5 171 3.74e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04666.t1 AT3G63010 72.911 347 89 3 1 347 1 342 0.0 544
MS.gene04666.t1 AT5G27320 68.946 351 100 6 1 350 1 343 1.22e-177 496
MS.gene04666.t1 AT3G05120 66.952 351 109 5 1 350 1 345 1.12e-173 486
MS.gene04666.t1 AT5G23530 34.320 338 195 7 15 347 12 327 1.70e-61 200
MS.gene04666.t1 AT5G16080 31.699 306 174 7 59 349 59 344 2.18e-43 153
MS.gene04666.t1 AT1G68620 34.836 244 133 6 69 306 65 288 1.49e-41 148
MS.gene04666.t1 AT5G62180 30.597 268 170 5 32 294 15 271 1.23e-39 143
MS.gene04666.t1 AT2G45600 32.751 229 140 3 106 322 66 292 1.48e-34 129
MS.gene04666.t1 AT5G14310 32.707 266 134 5 106 329 151 413 1.55e-33 129
MS.gene04666.t1 AT1G47480 29.907 321 193 11 33 347 16 310 2.80e-33 125
MS.gene04666.t1 AT2G03550 31.481 324 176 12 39 347 19 311 7.41e-33 124
MS.gene04666.t1 AT1G19190 35.439 285 141 14 53 321 30 287 1.69e-32 124
MS.gene04666.t1 AT5G06570 35.338 266 155 9 96 347 64 326 6.59e-32 122
MS.gene04666.t1 AT5G06570 35.338 266 155 9 96 347 64 326 6.59e-32 122
MS.gene04666.t1 AT5G06570 35.338 266 155 9 96 347 79 341 1.03e-31 122
MS.gene04666.t1 AT5G06570 35.338 266 155 9 96 347 79 341 1.03e-31 122
MS.gene04666.t1 AT2G45610 28.720 289 179 6 69 347 53 324 4.89e-30 117
MS.gene04666.t1 AT3G27320 29.310 348 169 9 56 329 51 395 1.19e-29 118
MS.gene04666.t1 AT3G27320 31.399 293 143 6 78 329 152 427 5.11e-29 117
MS.gene04666.t1 AT3G48690 32.463 268 147 11 50 302 27 275 1.86e-27 110
MS.gene04666.t1 AT3G48700 33.704 270 139 13 50 299 27 276 2.91e-27 110
MS.gene04666.t1 AT1G49650 32.090 268 154 8 53 311 86 334 1.83e-26 108
MS.gene04666.t1 AT1G49640 29.183 257 154 9 56 308 40 272 1.56e-24 102
MS.gene04666.t1 AT1G49660 30.303 264 159 6 53 308 30 276 1.38e-23 99.8

Find 72 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATTTCTGTTGTACTTAA+TGG 0.166515 7.3:+16881768 None:intergenic
TGGCTTATGTGGATGGTTTA+AGG 0.186814 7.3:-16881161 MS.gene04666:CDS
GTGGATGGTTTAAGGAATTT+TGG 0.215905 7.3:-16881153 MS.gene04666:CDS
TGATCATTTCTATTGCCTTA+TGG 0.245202 7.3:-16881067 MS.gene04666:CDS
ATTCTTCTTCATCCTTTGTT+TGG 0.284217 7.3:-16881411 MS.gene04666:CDS
GCAGAAGGAATTGAAGTGTT+AGG 0.327890 7.3:-16881438 MS.gene04666:CDS
AAAAGTCTTGTTTGTGTGGC+TGG 0.337676 7.3:-16881210 MS.gene04666:CDS
CAATAGAAATGATCATTATT+AGG 0.341519 7.3:+16881075 None:intergenic
TGGTCTCTATCTTCACCTTC+TGG 0.343587 7.3:+16881297 None:intergenic
AATGGGTGAAGTCAAGAAAA+TGG 0.346501 7.3:-16881563 MS.gene04666:CDS
GATTCTGAAATGAGATTAGA+TGG 0.348082 7.3:-16881372 MS.gene04666:CDS
TCTTGTTTGTGTGGCTGGTT+TGG 0.349537 7.3:-16881205 MS.gene04666:CDS
TACAAGATCGTGATTGGTAT+TGG 0.350184 7.3:-16881332 MS.gene04666:CDS
TACCTTAAGGAGGCTACTAT+TGG 0.351911 7.3:-16881111 MS.gene04666:CDS
TGTTAAACTTCTTTACCTTA+AGG 0.362269 7.3:-16881124 MS.gene04666:CDS
CTTCTTCATCCTTTGTTTGG+TGG 0.365813 7.3:-16881408 MS.gene04666:CDS
AAGTAATAGTATTAACAGTT+AGG 0.372219 7.3:+16881021 None:intergenic
TTCAGAAAATGAATCTCAAT+GGG 0.401908 7.3:-16881811 MS.gene04666:CDS
AGTTCTTGATTTCCTCCATA+AGG 0.402550 7.3:+16881052 None:intergenic
TTAGATTACAAGATCGTGAT+TGG 0.411360 7.3:-16881338 MS.gene04666:CDS
AAAGTTTATGTTTACATGGC+AGG 0.415400 7.3:-16881519 MS.gene04666:CDS
AGTCCCGGCAAATGCTATTC+CGG 0.421368 7.3:-16881907 MS.gene04666:CDS
AACCAATAGTAGCCTCCTTA+AGG 0.425272 7.3:+16881109 None:intergenic
TTGTAAAGCTGCTGTTGTTT+CGG 0.428335 7.3:-16881652 MS.gene04666:CDS
CACCCTGCTTGTAACCCGTT+TGG 0.430827 7.3:-16881276 MS.gene04666:CDS
CCTGTCATATTATTCTTCCA+TGG 0.439107 7.3:-16881744 MS.gene04666:CDS
TGTAACCCGTTTGGTCCAAA+AGG 0.441552 7.3:-16881267 MS.gene04666:CDS
TACATGGCAGGTGATAGTTC+TGG 0.444051 7.3:-16881507 MS.gene04666:CDS
CAGAAGGAATTGAAGTGTTA+GGG 0.454064 7.3:-16881437 MS.gene04666:CDS
CTTCAGAAAATGAATCTCAA+TGG 0.456710 7.3:-16881812 MS.gene04666:CDS
AGATTGGCAATTGGCTTATG+TGG 0.475777 7.3:-16881172 MS.gene04666:CDS
GGTTAATTACCGACGATCAC+CGG 0.491592 7.3:-16881631 MS.gene04666:CDS
GGACCAAACGGGTTACAAGC+AGG 0.492003 7.3:+16881273 None:intergenic
ATAAGAACATATGTATTCAA+TGG 0.496984 7.3:+16882014 None:intergenic
AAGTCAACCTCAGTGAATCC+AGG 0.499269 7.3:-16882411 MS.gene04666:CDS
GTAACCCGTTTGGTCCAAAA+GGG 0.503887 7.3:-16881266 MS.gene04666:CDS
ACCGACGATCACCGGAGTAT+CGG 0.504643 7.3:-16881623 MS.gene04666:CDS
CATGTGCTTATGAAGATGGC+TGG 0.506515 7.3:-16881596 MS.gene04666:CDS
TCCTAAAAGTCTTGTTTGTG+TGG 0.514991 7.3:-16881214 MS.gene04666:CDS
ATGGCTGGAATGCACTCAAA+TGG 0.521371 7.3:-16881581 MS.gene04666:CDS
AAAGAAAGTTTATGTTTACA+TGG 0.521453 7.3:-16881523 MS.gene04666:CDS
TTTCCATGTGCTTATGAAGA+TGG 0.528955 7.3:-16881600 MS.gene04666:CDS
TGGCAATTGGCTTATGTGGA+TGG 0.532054 7.3:-16881168 MS.gene04666:CDS
CAAGAAACTCTGCTAAATCT+CGG 0.546001 7.3:+16881937 None:intergenic
GTTTCTTGACAGAAAAGTCC+CGG 0.552120 7.3:-16881922 MS.gene04666:CDS
TCGACCGGAATAGCATTTGC+CGG 0.555684 7.3:+16881903 None:intergenic
CGACCGGAATAGCATTTGCC+GGG 0.556973 7.3:+16881904 None:intergenic
GACCAAACGGGTTACAAGCA+GGG 0.558028 7.3:+16881274 None:intergenic
CAAGAAAATGGCTTCAAAGT+GGG 0.561680 7.3:-16881551 MS.gene04666:CDS
GCAAATGCTATTCCGGTCGA+TGG 0.562535 7.3:-16881900 MS.gene04666:CDS
TGGCTGGAATGCACTCAAAT+GGG 0.565881 7.3:-16881580 MS.gene04666:CDS
ACCGATACTCCGGTGATCGT+CGG 0.572460 7.3:+16881622 None:intergenic
TAAACTTCTTTACCTTAAGG+AGG 0.574148 7.3:-16881121 MS.gene04666:CDS
AATCTTCTAAGAAGAGCAGA+TGG 0.579373 7.3:-16881969 MS.gene04666:CDS
TACAACTAACCAGACATGAC+TGG 0.594054 7.3:-16882442 None:intergenic
TCATTTCTATTGCCTTATGG+AGG 0.599565 7.3:-16881064 MS.gene04666:CDS
TGTAGCAGTAAAAGCTTGTG+AGG 0.600327 7.3:-16881466 MS.gene04666:CDS
CCATGGAAGAATAATATGAC+AGG 0.602409 7.3:+16881744 None:intergenic
CAGCCATCTTCATAAGCACA+TGG 0.605205 7.3:+16881597 None:intergenic
AATGAAAAGACTCCATCGAC+CGG 0.605436 7.3:+16881888 None:intergenic
GTCATATTATTCTTCCATGG+TGG 0.613549 7.3:-16881741 MS.gene04666:CDS
TCAAGAAAATGGCTTCAAAG+TGG 0.615492 7.3:-16881552 MS.gene04666:CDS
GCACATGGAAACCGATACTC+CGG 0.625683 7.3:+16881612 None:intergenic
ATGGCAGGTGATAGTTCTGG+TGG 0.630946 7.3:-16881504 MS.gene04666:CDS
GATCATATCGAACGAAACAC+CGG 0.631025 7.3:-16881864 MS.gene04666:CDS
AGTCAACCTCAGTGAATCCA+GGG 0.636231 7.3:-16882410 MS.gene04666:intron
ATAAACACGATTGAATAGAC+CGG 0.640482 7.3:+16881845 None:intergenic
TAACCCGTTTGGTCCAAAAG+GGG 0.642733 7.3:-16881265 MS.gene04666:CDS
AATGAGAGAAGCTTCCACCA+TGG 0.652602 7.3:+16881727 None:intergenic
TCAGAAAATGAATCTCAATG+GGG 0.736042 7.3:-16881810 MS.gene04666:CDS
ATATTACCCTGGATTCACTG+AGG 0.739496 7.3:+16882404 None:intergenic
CAAACGGGTTACAAGCAGGG+TGG 0.789743 7.3:+16881277 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAACAAAATTTTCTAAGA+TGG + chr7.3:16881089-16881108 None:intergenic 15.0%
!! AAAGAAAGTTTATGTTTACA+TGG - chr7.3:16881936-16881955 MS.gene04666:CDS 20.0%
!! AAGAAAAATGAAACAAATCT+TGG + chr7.3:16881361-16881380 None:intergenic 20.0%
!! AGAAAAATGAAACAAATCTT+GGG + chr7.3:16881360-16881379 None:intergenic 20.0%
!! ATAAGAACATATGTATTCAA+TGG + chr7.3:16881448-16881467 None:intergenic 20.0%
!! CAATAGAAATGATCATTATT+AGG + chr7.3:16882387-16882406 None:intergenic 20.0%
!! TAAACTTTAACATATGTGAT+TGG - chr7.3:16881165-16881184 MS.gene04666:CDS 20.0%
!!! ATCATGTAATATTTTTCTGT+GGG - chr7.3:16881200-16881219 MS.gene04666:CDS 20.0%
! AAAGGGGAAAAAAATCTTAA+AGG - chr7.3:16882210-16882229 MS.gene04666:intron 25.0%
! ACAATTTCTGTTGTACTTAA+TGG + chr7.3:16881694-16881713 None:intergenic 25.0%
! GCAAAACAATAACTTTAGAT+TGG + chr7.3:16881414-16881433 None:intergenic 25.0%
! TCATTATGTGTCACAATAAT+TGG - chr7.3:16881318-16881337 MS.gene04666:CDS 25.0%
! TGTTAAACTTCTTTACCTTA+AGG - chr7.3:16882335-16882354 MS.gene04666:intron 25.0%
! TTCAGAAAATGAATCTCAAT+GGG - chr7.3:16881648-16881667 MS.gene04666:CDS 25.0%
!! AGTTTCTTCTTTTTATGTGT+TGG - chr7.3:16881260-16881279 MS.gene04666:CDS 25.0%
!! GATCATGTAATATTTTTCTG+TGG - chr7.3:16881199-16881218 MS.gene04666:CDS 25.0%
!! GTTTCTTCTTTTTATGTGTT+GGG - chr7.3:16881261-16881280 MS.gene04666:CDS 25.0%
!! TCTATGATACATTTTGTAGA+AGG - chr7.3:16881773-16881792 MS.gene04666:CDS 25.0%
!!! TTAAGATTTTTTTCCCCTTT+TGG + chr7.3:16882210-16882229 None:intergenic 25.0%
!!! TTTGCTTTGTTTTTGATGTT+GGG - chr7.3:16881128-16881147 MS.gene04666:CDS 25.0%
AAAGTTTATGTTTACATGGC+AGG - chr7.3:16881940-16881959 MS.gene04666:CDS 30.0%
ATAAACACGATTGAATAGAC+CGG + chr7.3:16881617-16881636 None:intergenic 30.0%
ATTCTTCTTCATCCTTTGTT+TGG - chr7.3:16882048-16882067 MS.gene04666:intron 30.0%
CTTCAGAAAATGAATCTCAA+TGG - chr7.3:16881647-16881666 MS.gene04666:CDS 30.0%
TAAACTTCTTTACCTTAAGG+AGG - chr7.3:16882338-16882357 MS.gene04666:intron 30.0%
TCAGAAAATGAATCTCAATG+GGG - chr7.3:16881649-16881668 MS.gene04666:CDS 30.0%
TGATCATTTCTATTGCCTTA+TGG - chr7.3:16882392-16882411 MS.gene04666:intron 30.0%
TTAGATTACAAGATCGTGAT+TGG - chr7.3:16882121-16882140 MS.gene04666:intron 30.0%
! GGTTTCTTGTTATGGTAAAT+TGG - chr7.3:16881282-16881301 MS.gene04666:CDS 30.0%
! GTTTGGATCTTTTACAAGAT+TGG - chr7.3:16882271-16882290 MS.gene04666:intron 30.0%
! TCTTTTACAAGATTGGCAAT+TGG - chr7.3:16882278-16882297 MS.gene04666:intron 30.0%
!! GATTCATTTTCTGAAGATGA+TGG + chr7.3:16881643-16881662 None:intergenic 30.0%
!! GATTCTGAAATGAGATTAGA+TGG - chr7.3:16882087-16882106 MS.gene04666:intron 30.0%
!!! ATATTTTTCTGTGGGTTAGT+TGG - chr7.3:16881208-16881227 MS.gene04666:CDS 30.0%
!!! GTTTGCTTTGTTTTTGATGT+TGG - chr7.3:16881127-16881146 MS.gene04666:CDS 30.0%
AATGGGTGAAGTCAAGAAAA+TGG - chr7.3:16881896-16881915 MS.gene04666:CDS 35.0%
AGTTCTTGATTTCCTCCATA+AGG + chr7.3:16882410-16882429 None:intergenic 35.0%
ATGTGTTGGGTTTCTTGTTA+TGG - chr7.3:16881274-16881293 MS.gene04666:CDS 35.0%
CAAGAAAATGGCTTCAAAGT+GGG - chr7.3:16881908-16881927 MS.gene04666:CDS 35.0%
CAAGAAACTCTGCTAAATCT+CGG + chr7.3:16881525-16881544 None:intergenic 35.0%
CCATGGAAGAATAATATGAC+AGG + chr7.3:16881718-16881737 None:intergenic 35.0%
CCTGTCATATTATTCTTCCA+TGG - chr7.3:16881715-16881734 MS.gene04666:CDS 35.0%
GTCATATTATTCTTCCATGG+TGG - chr7.3:16881718-16881737 MS.gene04666:CDS 35.0%
TACAAGATCGTGATTGGTAT+TGG - chr7.3:16882127-16882146 MS.gene04666:intron 35.0%
TCAAGAAAATGGCTTCAAAG+TGG - chr7.3:16881907-16881926 MS.gene04666:CDS 35.0%
TCATTTCTATTGCCTTATGG+AGG - chr7.3:16882395-16882414 MS.gene04666:intron 35.0%
TGGAAGATGATATATTACCC+TGG + chr7.3:16881069-16881088 None:intergenic 35.0%
TTTCCATGTGCTTATGAAGA+TGG - chr7.3:16881859-16881878 MS.gene04666:CDS 35.0%
! AATCTTCTAAGAAGAGCAGA+TGG - chr7.3:16881490-16881509 MS.gene04666:CDS 35.0%
! CAGAAGGAATTGAAGTGTTA+GGG - chr7.3:16882022-16882041 MS.gene04666:CDS 35.0%
! GTGGATGGTTTAAGGAATTT+TGG - chr7.3:16882306-16882325 MS.gene04666:intron 35.0%
! TTGTAAAGCTGCTGTTGTTT+CGG - chr7.3:16881807-16881826 MS.gene04666:CDS 35.0%
!! TCCTAAAAGTCTTGTTTGTG+TGG - chr7.3:16882245-16882264 MS.gene04666:intron 35.0%
!!! CTTTTTTCACCACCAAACAA+AGG + chr7.3:16882063-16882082 None:intergenic 35.0%
AACCAATAGTAGCCTCCTTA+AGG + chr7.3:16882353-16882372 None:intergenic 40.0%
AATGAAAAGACTCCATCGAC+CGG + chr7.3:16881574-16881593 None:intergenic 40.0%
ATATTACCCTGGATTCACTG+AGG + chr7.3:16881058-16881077 None:intergenic 40.0%
GATCATATCGAACGAAACAC+CGG - chr7.3:16881595-16881614 MS.gene04666:CDS 40.0%
GTTTCTTGACAGAAAAGTCC+CGG - chr7.3:16881537-16881556 MS.gene04666:CDS 40.0%
TACCTTAAGGAGGCTACTAT+TGG - chr7.3:16882348-16882367 MS.gene04666:intron 40.0%
TGGCTTATGTGGATGGTTTA+AGG - chr7.3:16882298-16882317 MS.gene04666:intron 40.0%
TGTAGCAGTAAAAGCTTGTG+AGG - chr7.3:16881993-16882012 MS.gene04666:CDS 40.0%
! AGATTGGCAATTGGCTTATG+TGG - chr7.3:16882287-16882306 MS.gene04666:intron 40.0%
! CTTCTTCATCCTTTGTTTGG+TGG - chr7.3:16882051-16882070 MS.gene04666:intron 40.0%
! GCAGAAGGAATTGAAGTGTT+AGG - chr7.3:16882021-16882040 MS.gene04666:CDS 40.0%
! GCCACACAAACAAGACTTTT+AGG + chr7.3:16882249-16882268 None:intergenic 40.0%
! TTTTCCCCTTTTGGACCAAA+CGG + chr7.3:16882201-16882220 None:intergenic 40.0%
!! AAAAGTCTTGTTTGTGTGGC+TGG - chr7.3:16882249-16882268 MS.gene04666:intron 40.0%
!! GTGGGTTAGTTGGCTTAATT+TGG - chr7.3:16881218-16881237 MS.gene04666:CDS 40.0%
!!! TGGAGAGCTTTTTTACCAGA+AGG - chr7.3:16882147-16882166 MS.gene04666:intron 40.0%
AAGTCAACCTCAGTGAATCC+AGG - chr7.3:16881048-16881067 MS.gene04666:CDS 45.0%
AATGAGAGAAGCTTCCACCA+TGG + chr7.3:16881735-16881754 None:intergenic 45.0%
AGTCAACCTCAGTGAATCCA+GGG - chr7.3:16881049-16881068 MS.gene04666:CDS 45.0%
ATGGCTGGAATGCACTCAAA+TGG - chr7.3:16881878-16881897 MS.gene04666:CDS 45.0%
CAGCCATCTTCATAAGCACA+TGG + chr7.3:16881865-16881884 None:intergenic 45.0%
CATGTGCTTATGAAGATGGC+TGG - chr7.3:16881863-16881882 MS.gene04666:CDS 45.0%
GCTTGTGAGGAAAAAGCAGA+AGG - chr7.3:16882006-16882025 MS.gene04666:CDS 45.0%
GGTTAATTACCGACGATCAC+CGG - chr7.3:16881828-16881847 MS.gene04666:CDS 45.0%
GTAACCCGTTTGGTCCAAAA+GGG - chr7.3:16882193-16882212 MS.gene04666:intron 45.0%
TAACCCGTTTGGTCCAAAAG+GGG - chr7.3:16882194-16882213 MS.gene04666:intron 45.0%
TACATGGCAGGTGATAGTTC+TGG - chr7.3:16881952-16881971 MS.gene04666:CDS 45.0%
TGGCTGGAATGCACTCAAAT+GGG - chr7.3:16881879-16881898 MS.gene04666:CDS 45.0%
TGGTCTCTATCTTCACCTTC+TGG + chr7.3:16882165-16882184 None:intergenic 45.0%
TGTAACCCGTTTGGTCCAAA+AGG - chr7.3:16882192-16882211 MS.gene04666:intron 45.0%
! TCTTGTTTGTGTGGCTGGTT+TGG - chr7.3:16882254-16882273 MS.gene04666:intron 45.0%
! TGGCAATTGGCTTATGTGGA+TGG - chr7.3:16882291-16882310 MS.gene04666:intron 45.0%
! TTTCCCCTTTTGGACCAAAC+GGG + chr7.3:16882200-16882219 None:intergenic 45.0%
AGTCCCGGCAAATGCTATTC+CGG - chr7.3:16881552-16881571 MS.gene04666:CDS 50.0%
GCAAATGCTATTCCGGTCGA+TGG - chr7.3:16881559-16881578 MS.gene04666:CDS 50.0%
GCACATGGAAACCGATACTC+CGG + chr7.3:16881850-16881869 None:intergenic 50.0%
TCGACCGGAATAGCATTTGC+CGG + chr7.3:16881559-16881578 None:intergenic 50.0%
!! ATGGCAGGTGATAGTTCTGG+TGG - chr7.3:16881955-16881974 MS.gene04666:CDS 50.0%
!! GACCAAACGGGTTACAAGCA+GGG + chr7.3:16882188-16882207 None:intergenic 50.0%
ACCGACGATCACCGGAGTAT+CGG - chr7.3:16881836-16881855 MS.gene04666:CDS 55.0%
ACCGATACTCCGGTGATCGT+CGG + chr7.3:16881840-16881859 None:intergenic 55.0%
CACCCTGCTTGTAACCCGTT+TGG - chr7.3:16882183-16882202 MS.gene04666:intron 55.0%
CGACCGGAATAGCATTTGCC+GGG + chr7.3:16881558-16881577 None:intergenic 55.0%
!! CAAACGGGTTACAAGCAGGG+TGG + chr7.3:16882185-16882204 None:intergenic 55.0%
!! GGACCAAACGGGTTACAAGC+AGG + chr7.3:16882189-16882208 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 16881032 16882449 16881032 ID=MS.gene04666
chr7.3 mRNA 16881032 16882449 16881032 ID=MS.gene04666.t1;Parent=MS.gene04666
chr7.3 exon 16882411 16882449 16882411 ID=MS.gene04666.t1.exon1;Parent=MS.gene04666.t1
chr7.3 CDS 16882411 16882449 16882411 ID=cds.MS.gene04666.t1;Parent=MS.gene04666.t1
chr7.3 exon 16881032 16882045 16881032 ID=MS.gene04666.t1.exon2;Parent=MS.gene04666.t1
chr7.3 CDS 16881032 16882045 16881032 ID=cds.MS.gene04666.t1;Parent=MS.gene04666.t1
Gene Sequence

>MS.gene04666

ATGACTGGTACTAATGAAGTCAACCTCAGTGAATCCAGGGTAATATATCATCTTCCATCTTAGAAAATTTTGTTTTTTTTGTTTAAAGCTTTGTTGTTTGCTTTGTTTTTGATGTTGGGTTTTCAAAAAGTTCTAAACTTTAACATATGTGATTGGTGTTTTGTTAAGATCATGTAATATTTTTCTGTGGGTTAGTTGGCTTAATTTGGTTTTTGTTTTCTACTTTCTAGTTTCTTCTTTTTATGTGTTGGGTTTCTTGTTATGGTAAATTGGAAAGATTTGATTTTCATTATGTGTCACAATAATTGGAAATTTTTGTCTTTTACCCAAGATTTGTTTCATTTTTCTTTCTTCTGCAATGATTCAACACAAAAAAAAACCAATCTAAAGTTATTGTTTTGCAGAGTGTTGTTCCATTGAATACATATGTTCTTATCTCAAATTTCAAGCTTGCTTACAATCTTCTAAGAAGAGCAGATGGAACATTCAACCGAGATTTAGCAGAGTTTCTTGACAGAAAAGTCCCGGCAAATGCTATTCCGGTCGATGGAGTCTTTTCATTCGATCATATCGAACGAAACACCGGTCTATTCAATCGTGTTTATCTACCATCATCTTCAGAAAATGAATCTCAATGGGGTGTCAAAGATTTAGAGAAACCATTAAGTACAACAGAAATTGTTCCTGTCATATTATTCTTCCATGGTGGAAGCTTCTCTCATTCTTCAGCAAACAGTGCTATCTATGATACATTTTGTAGAAGGTTAGTAAGTGTTTGTAAAGCTGCTGTTGTTTCGGTTAATTACCGACGATCACCGGAGTATCGGTTTCCATGTGCTTATGAAGATGGCTGGAATGCACTCAAATGGGTGAAGTCAAGAAAATGGCTTCAAAGTGGGAAAGAAAAGAAAGTTTATGTTTACATGGCAGGTGATAGTTCTGGTGGAAACATTGTTCATCATGTAGCAGTAAAAGCTTGTGAGGAAAAAGCAGAAGGAATTGAAGTGTTAGGGAACATTCTTCTTCATCCTTTGTTTGGTGGTGAAAAAAGAACAGATTCTGAAATGAGATTAGATGGAAAGTACTTTGTTAGATTACAAGATCGTGATTGGTATTGGAGAGCTTTTTTACCAGAAGGTGAAGATAGAGACCACCCTGCTTGTAACCCGTTTGGTCCAAAAGGGGAAAAAAATCTTAAAGGACTTGATAAATTTCCTAAAAGTCTTGTTTGTGTGGCTGGTTTGGATCTTTTACAAGATTGGCAATTGGCTTATGTGGATGGTTTAAGGAATTTTGGTCAAGATGTTAAACTTCTTTACCTTAAGGAGGCTACTATTGGTTTTTACTTCTTGCCTAATAATGATCATTTCTATTGCCTTATGGAGGAAATCAAGAACTTTGTTAATCCTAACTGTTAA
Protein sequence

>MS.gene04666.t1

MTGTNEVNLSESRSVVPLNTYVLISNFKLAYNLLRRADGTFNRDLAEFLDRKVPANAIPVDGVFSFDHIERNTGLFNRVYLPSSSENESQWGVKDLEKPLSTTEIVPVILFFHGGSFSHSSANSAIYDTFCRRLVSVCKAAVVSVNYRRSPEYRFPCAYEDGWNALKWVKSRKWLQSGKEKKVYVYMAGDSSGGNIVHHVAVKACEEKAEGIEVLGNILLHPLFGGEKRTDSEMRLDGKYFVRLQDRDWYWRAFLPEGEDRDHPACNPFGPKGEKNLKGLDKFPKSLVCVAGLDLLQDWQLAYVDGLRNFGQDVKLLYLKEATIGFYFLPNNDHFYCLMEEIKNFVNPNC