AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21543

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21543.t1 MTR_1g089310 98.547 344 5 0 1 344 17 360 0.0 711
MS.gene21543.t1 MTR_1g089310 98.547 344 5 0 1 344 1 344 0.0 709
MS.gene21543.t1 MTR_7g093950 84.000 350 50 4 1 344 1 350 0.0 611
MS.gene21543.t1 MTR_1g082210 71.884 345 95 2 1 344 1 344 0.0 530
MS.gene21543.t1 MTR_6g065400 39.528 339 196 3 7 341 1 334 6.77e-80 247
MS.gene21543.t1 MTR_6g065110 40.426 329 187 3 17 341 12 335 3.76e-78 243
MS.gene21543.t1 MTR_6g065120 38.988 336 195 6 10 340 5 335 1.39e-76 239
MS.gene21543.t1 MTR_6g065370 38.690 336 197 3 10 341 4 334 1.46e-76 239
MS.gene21543.t1 MTR_6g065270 37.092 337 205 5 9 340 1 335 3.95e-73 230
MS.gene21543.t1 MTR_6g065430 36.310 336 196 4 11 340 4 327 2.49e-67 215
MS.gene21543.t1 MTR_6g065340 35.103 339 192 5 10 341 12 329 1.21e-62 203
MS.gene21543.t1 MTR_4g050140 34.694 294 159 6 61 341 43 316 2.57e-44 155
MS.gene21543.t1 MTR_1g012430 34.701 268 153 6 92 340 43 307 2.38e-41 147
MS.gene21543.t1 MTR_3g012440 36.975 238 137 4 96 322 79 314 9.00e-41 146
MS.gene21543.t1 MTR_7g107040 36.522 230 131 4 105 321 69 296 7.50e-40 143
MS.gene21543.t1 MTR_1g111280 37.295 244 132 6 98 323 81 321 1.27e-39 143
MS.gene21543.t1 MTR_7g406880 33.218 289 157 8 61 325 54 330 7.53e-39 141
MS.gene21543.t1 MTR_1g102170 40.086 232 123 8 89 308 80 307 6.17e-38 138
MS.gene21543.t1 MTR_8g035540 32.867 286 151 9 37 301 13 278 1.93e-36 134
MS.gene21543.t1 MTR_6g464510 40.909 176 95 4 97 264 74 248 2.64e-36 134
MS.gene21543.t1 MTR_7g056207 33.103 290 162 10 69 344 82 353 5.66e-35 131
MS.gene21543.t1 MTR_4g086480 32.540 252 150 8 102 340 74 318 1.69e-34 129
MS.gene21543.t1 MTR_3g450680 32.107 299 163 9 70 341 47 332 2.91e-34 129
MS.gene21543.t1 MTR_6g464120 31.788 302 173 10 32 321 23 303 3.74e-34 132
MS.gene21543.t1 MTR_6g022710 33.977 259 130 5 105 323 160 417 3.75e-34 130
MS.gene21543.t1 MTR_4g086510 35.200 250 143 7 105 341 75 318 5.86e-34 127
MS.gene21543.t1 MTR_0007s0520 36.508 189 109 4 87 265 59 246 1.52e-33 126
MS.gene21543.t1 MTR_6g464320 34.914 232 139 5 103 323 79 309 2.04e-33 126
MS.gene21543.t1 MTR_1g102160 36.458 192 110 3 84 264 57 247 5.03e-33 125
MS.gene21543.t1 MTR_6g464280 36.697 218 124 6 103 308 78 293 7.15e-33 124
MS.gene21543.t1 MTR_3g101420 29.677 310 173 9 38 334 20 297 9.02e-33 124
MS.gene21543.t1 MTR_4g086470 31.727 249 150 7 105 340 76 317 1.60e-31 121
MS.gene21543.t1 MTR_5g005920 31.118 331 181 13 25 334 9 313 5.13e-31 120
MS.gene21543.t1 MTR_8g035520 32.051 234 136 6 100 316 74 301 1.34e-30 119
MS.gene21543.t1 MTR_8g040860 30.970 268 163 7 70 322 47 307 4.39e-30 117
MS.gene21543.t1 MTR_8g035500 29.808 312 181 11 25 321 8 296 1.94e-29 115
MS.gene21543.t1 MTR_1g105020 29.831 295 169 8 38 314 21 295 3.66e-29 114
MS.gene21543.t1 MTR_7g051020 33.969 262 147 8 100 341 71 326 2.76e-28 112
MS.gene21543.t1 MTR_5g005900 31.304 230 134 7 95 305 64 288 9.91e-28 111
MS.gene21543.t1 MTR_4g086540 29.304 273 149 8 38 304 31 265 2.94e-27 109
MS.gene21543.t1 MTR_1g104933 33.206 262 149 7 98 341 64 317 1.22e-26 107
MS.gene21543.t1 MTR_5g005850 28.221 326 188 11 38 340 26 328 5.01e-26 106
MS.gene21543.t1 MTR_1g104930 31.818 264 154 7 98 341 66 323 5.53e-24 100
MS.gene21543.t1 MTR_0007s0480 38.202 89 48 1 184 265 4 92 7.71e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21543.t1 AT3G63010 78.070 342 74 1 1 341 1 342 0.0 577
MS.gene21543.t1 AT5G27320 72.464 345 92 3 1 344 1 343 0.0 525
MS.gene21543.t1 AT3G05120 69.942 346 101 3 1 344 1 345 0.0 511
MS.gene21543.t1 AT5G23530 35.817 349 192 7 1 340 1 326 5.36e-65 209
MS.gene21543.t1 AT5G16080 35.985 264 143 6 100 343 87 344 3.00e-46 160
MS.gene21543.t1 AT1G68620 34.843 287 152 11 69 341 65 330 6.83e-45 157
MS.gene21543.t1 AT5G06570 37.551 245 136 6 93 321 62 305 1.48e-37 137
MS.gene21543.t1 AT5G06570 37.551 245 136 6 93 321 62 305 1.48e-37 137
MS.gene21543.t1 AT5G06570 37.551 245 136 6 93 321 77 320 2.36e-37 137
MS.gene21543.t1 AT5G06570 37.551 245 136 6 93 321 77 320 2.36e-37 137
MS.gene21543.t1 AT2G45600 34.783 253 142 7 84 316 43 292 1.75e-36 134
MS.gene21543.t1 AT1G47480 30.284 317 191 9 33 341 16 310 1.98e-36 134
MS.gene21543.t1 AT5G14310 33.962 265 127 6 105 323 151 413 1.77e-35 134
MS.gene21543.t1 AT5G62180 31.950 241 146 6 32 264 15 245 2.43e-35 131
MS.gene21543.t1 AT1G19190 35.106 282 140 11 53 315 30 287 3.23e-34 128
MS.gene21543.t1 AT2G45610 34.137 249 147 7 105 340 79 323 1.30e-31 121
MS.gene21543.t1 AT3G48700 33.648 318 165 14 50 340 27 325 1.76e-31 121
MS.gene21543.t1 AT2G03550 31.153 321 175 13 39 341 19 311 4.40e-31 119
MS.gene21543.t1 AT3G48690 33.099 284 151 10 39 304 19 281 1.17e-30 119
MS.gene21543.t1 AT3G27320 32.830 265 129 7 105 323 134 395 4.62e-30 119
MS.gene21543.t1 AT3G27320 32.830 265 129 7 105 323 166 427 6.61e-30 119
MS.gene21543.t1 AT1G49650 31.298 262 156 7 53 304 86 333 3.02e-27 110
MS.gene21543.t1 AT1G49640 32.039 206 129 7 105 304 74 274 2.64e-26 107
MS.gene21543.t1 AT1G49660 28.947 266 169 7 49 303 21 277 1.30e-24 102

Find 95 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACATCTTGGTTAGATTTC+TGG 0.175894 1.1:-65561002 None:intergenic
ATGGTTGGAATGCTCTAAAT+TGG 0.260815 1.1:+65560578 MS.gene21543:CDS
GATCATGTCGATAGAAATTC+AGG 0.292227 1.1:+65560298 MS.gene21543:CDS
TTGTTACCTTAGATTCATTA+AGG 0.302473 1.1:-65559405 None:intergenic
CCTTGTTTGTGTGGCTGGTT+TGG 0.311025 1.1:+65560939 MS.gene21543:CDS
GGTAAAGAAGTTTGACATCT+TGG 0.317354 1.1:-65561014 None:intergenic
ATTCTTCTCCATCCACTCTT+TGG 0.317740 1.1:+65560739 MS.gene21543:CDS
AACCAATAGTGGCTTCCTTT+AGG 0.329579 1.1:-65561035 None:intergenic
AAAAGCCTTGTTTGTGTGGC+TGG 0.339972 1.1:+65560934 MS.gene21543:CDS
ATTGTAAGCAAGCTTGAAAT+TGG 0.347441 1.1:-65560173 None:intergenic
CAATAGAAATGATCATTATT+AGG 0.355983 1.1:-65561069 None:intergenic
TACCTAAAGGAAGCCACTAT+TGG 0.357783 1.1:+65561033 MS.gene21543:CDS
GCCAATACAATTCCTGTAGA+TGG 0.359532 1.1:+65560262 MS.gene21543:CDS
AGTCAACCTTAATGAATCTA+AGG 0.367754 1.1:+65559399 MS.gene21543:CDS
TGATCTCTATCTTCCCCTTC+AGG 0.371490 1.1:-65560853 None:intergenic
GTTTGGATCTTCTACAAGAT+TGG 0.373570 1.1:+65560956 MS.gene21543:CDS
TTCTCCATCCACTCTTTGGT+GGG 0.385254 1.1:+65560743 MS.gene21543:CDS
TGCAAGATCGTGATTGGTAT+TGG 0.387188 1.1:+65560818 MS.gene21543:CDS
AACAGAATCGGAGAAGAAAC+TGG 0.395775 1.1:+65560774 MS.gene21543:CDS
CCTAAAGGTAAAAGCCTTGT+AGG 0.397562 1.1:+65560898 MS.gene21543:CDS
CTCCAAGGTTTATGCTTACA+TGG 0.407235 1.1:+65560636 MS.gene21543:CDS
CTCCAGAGAATGTTACTACT+TGG 0.411618 1.1:+65560347 MS.gene21543:CDS
CTTCTCCATCCACTCTTTGG+TGG 0.413791 1.1:+65560742 MS.gene21543:CDS
GTGTTGTTCCTCTCAATACT+TGG 0.417333 1.1:+65560140 MS.gene21543:CDS
CATCCTGCTTGTAATCCATT+TGG 0.419839 1.1:+65560874 MS.gene21543:CDS
CCCCAAGTAGTAACATTCTC+TGG 0.421392 1.1:-65560349 None:intergenic
TGTAATCCATTTGGTCCTAA+AGG 0.430276 1.1:+65560883 MS.gene21543:CDS
CTCTAAATTGGGTCAAATCA+AGG 0.447638 1.1:+65560590 MS.gene21543:CDS
AAGGTTTATGCTTACATGGC+TGG 0.447846 1.1:+65560640 MS.gene21543:CDS
TATCCGTGTGCTTACGAAGA+TGG 0.455575 1.1:+65560559 MS.gene21543:CDS
TACATGGCTGGTGATAGTTC+CGG 0.457192 1.1:+65560652 MS.gene21543:CDS
TGTCAAACTTCTTTACCTAA+AGG 0.457617 1.1:+65561020 MS.gene21543:CDS
CCCTAAAAGCCTTGTTTGTG+TGG 0.460173 1.1:+65560930 MS.gene21543:CDS
TTATTGTTTCTGTAAACTAC+AGG 0.460275 1.1:+65560518 MS.gene21543:CDS
TCTTCTACAAGATTGGCAAT+TGG 0.460325 1.1:+65560963 MS.gene21543:CDS
GACAATCTCTGTTGTGCTCA+AGG 0.461484 1.1:-65560392 None:intergenic
TCTACAGGAATTGTATTGGC+TGG 0.475234 1.1:-65560259 None:intergenic
TGAGGTTGCAAGATCGTGAT+TGG 0.487767 1.1:+65560812 MS.gene21543:CDS
TCCATCTACAGGAATTGTAT+TGG 0.489416 1.1:-65560263 None:intergenic
TCTCCATCCACTCTTTGGTG+GGG 0.492850 1.1:+65560744 MS.gene21543:CDS
GAGGAAGATGTTGAGGTACT+AGG 0.496473 1.1:+65560712 MS.gene21543:CDS
AAAGAGAATACTCCATCTAC+AGG 0.500048 1.1:-65560274 None:intergenic
CTCTCTATTGAATGTTCCAT+CGG 0.504062 1.1:-65560209 None:intergenic
AACATAGACATAAACATGGC+TGG 0.504836 1.1:+65559367 None:intergenic
TGGTTGGAATGCTCTAAATT+GGG 0.506865 1.1:+65560579 MS.gene21543:CDS
GTAGTAACATTCTCTGGAGC+AGG 0.509928 1.1:-65560343 None:intergenic
TCGCCTTGTGAGCATGTGTA+AGG 0.514102 1.1:+65560492 MS.gene21543:CDS
TCCAGAGAATGTTACTACTT+GGG 0.521238 1.1:+65560348 MS.gene21543:CDS
TAGGGAACTTGAGTCCTACA+AGG 0.521873 1.1:-65560912 None:intergenic
TGGGGAGAAGCGAACAGAAT+CGG 0.526899 1.1:+65560762 MS.gene21543:CDS
GGACCAAATGGATTACAAGC+AGG 0.530885 1.1:-65560877 None:intergenic
AGATTGGCAATTGGAATATG+TGG 0.530969 1.1:+65560972 MS.gene21543:CDS
CCTGTCATAATCTTCTTCCA+TGG 0.533405 1.1:+65560415 MS.gene21543:CDS
CTACAGGAATTGTATTGGCT+GGG 0.533816 1.1:-65560258 None:intergenic
TGGAAGTTTCTCTCATTCCT+CGG 0.536571 1.1:+65560438 MS.gene21543:CDS
TGTTGTTCCTCTCAATACTT+GGG 0.536632 1.1:+65560141 MS.gene21543:CDS
GTAGCAGTATATCAGCAGTT+AGG 0.539039 1.1:-65561123 None:intergenic
CGTGTGCTTACGAAGATGGT+TGG 0.544969 1.1:+65560563 MS.gene21543:CDS
AGAAATGATCATTATTAGGA+AGG 0.548626 1.1:-65561065 None:intergenic
CCAGAGAATGTTACTACTTG+GGG 0.553071 1.1:+65560349 MS.gene21543:CDS
CAGCCTTACACATGCTCACA+AGG 0.557141 1.1:-65560495 None:intergenic
CCATGGAAGAAGATTATGAC+AGG 0.559426 1.1:-65560415 None:intergenic
GCACACGGATACCGATGCTC+GGG 0.559770 1.1:-65560547 None:intergenic
CGTTGCGGTGAGAGCTGCCG+AGG 0.561498 1.1:+65560693 MS.gene21543:CDS
GTGATGAGCAATGTTGCCTC+CGG 0.563388 1.1:-65560671 None:intergenic
ACAATCTCTGTTGTGCTCAA+GGG 0.564360 1.1:-65560391 None:intergenic
AGCTGAGTTTCTTGACCGCA+AGG 0.568671 1.1:+65560234 MS.gene21543:CDS
AGCACACGGATACCGATGCT+CGG 0.568740 1.1:-65560548 None:intergenic
TAGTACCTCAACATCTTCCT+CGG 0.571640 1.1:-65560710 None:intergenic
AAGGAAGTAGAAACCAATAG+TGG 0.572842 1.1:-65561046 None:intergenic
TCTCCCCACCAAAGAGTGGA+TGG 0.573660 1.1:-65560747 None:intergenic
AGCTGCCGAGGAAGATGTTG+AGG 0.574921 1.1:+65560705 MS.gene21543:CDS
AATCTACTAAGACGCGCCGA+TGG 0.574971 1.1:+65560193 MS.gene21543:CDS
GAATCGGAGAAGAAACTGGA+TGG 0.579411 1.1:+65560778 MS.gene21543:CDS
CGCTTCTCCCCACCAAAGAG+TGG 0.583817 1.1:-65560751 None:intergenic
TTGTATTGGCTGGGACCTTG+CGG 0.585043 1.1:-65560249 None:intergenic
ACAGGAGATCACCCGAGCAT+CGG 0.608623 1.1:+65560536 MS.gene21543:CDS
CCAAACCAGCCACACAAACA+AGG 0.618481 1.1:-65560939 None:intergenic
TCAAAGTGGAAAGGACTCCA+AGG 0.619865 1.1:+65560621 MS.gene21543:CDS
GACATGGCTTCAAAGTGGAA+AGG 0.619941 1.1:+65560612 MS.gene21543:CDS
AGCCATGTAAGCATAAACCT+TGG 0.620826 1.1:-65560638 None:intergenic
ATTGGGTCAAATCAAGGACA+TGG 0.622807 1.1:+65560596 MS.gene21543:CDS
TCAAGGACATGGCTTCAAAG+TGG 0.623286 1.1:+65560607 MS.gene21543:CDS
TGGCAATTGGAATATGTGGA+AGG 0.626681 1.1:+65560976 MS.gene21543:CDS
GTCATAATCTTCTTCCATGG+TGG 0.626745 1.1:+65560418 MS.gene21543:CDS
CAACCATCTTCGTAAGCACA+CGG 0.632894 1.1:-65560562 None:intergenic
AGGCGTTTATCTCATTCATG+AGG 0.637102 1.1:-65561092 None:intergenic
ATAGATAGCACTATTAGCCG+AGG 0.651389 1.1:-65560455 None:intergenic
ATGGCTGGTGATAGTTCCGG+AGG 0.657964 1.1:+65560655 MS.gene21543:CDS
TACACATGCTCACAAGGCGA+CGG 0.659131 1.1:-65560489 None:intergenic
CAACATTGCTCATCACGTTG+CGG 0.669625 1.1:+65560678 MS.gene21543:CDS
ATAAGCACCCAAGTATTGAG+AGG 0.671972 1.1:-65560148 None:intergenic
AATGAGAGAAACTTCCACCA+TGG 0.679061 1.1:-65560432 None:intergenic
CATGGAAGAAGATTATGACA+GGG 0.698543 1.1:-65560414 None:intergenic
TCAGCAGTTAGGATGCACAA+AGG 0.743793 1.1:-65561112 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAGAAATGATCATTATT+AGG - chr1.1:65561072-65561091 None:intergenic 20.0%
!!! ACAACTTTCTTTGTTTTTTT+TGG + chr1.1:65559424-65559443 MS.gene21543:intron 20.0%
!!! ATAATTTCTTGCTTTTCTAA+AGG + chr1.1:65559896-65559915 MS.gene21543:intron 20.0%
!!! TAATTTCTTGCTTTTCTAAA+GGG + chr1.1:65559897-65559916 MS.gene21543:intron 20.0%
!!! TTCTAGAAACAAATTAAAGA+AGG - chr1.1:65559500-65559519 None:intergenic 20.0%
!!! TTCTATTTTTTCTTACATGT+AGG + chr1.1:65559735-65559754 MS.gene21543:intron 20.0%
! AATGAAGTGCATAATTCATA+TGG + chr1.1:65559977-65559996 MS.gene21543:intron 25.0%
! AGAAATGATCATTATTAGGA+AGG - chr1.1:65561068-65561087 None:intergenic 25.0%
! GCAAGAAATTATTGAAAAGA+AGG - chr1.1:65559888-65559907 None:intergenic 25.0%
! TATCAACTATGCATTTCATT+TGG - chr1.1:65560082-65560101 None:intergenic 25.0%
! TATTCTCTAATCAAAGCTTA+TGG - chr1.1:65560030-65560049 None:intergenic 25.0%
! TGAAAAGAAGGATCATAAAA+AGG - chr1.1:65559876-65559895 None:intergenic 25.0%
! TTGTTACCTTAGATTCATTA+AGG - chr1.1:65559408-65559427 None:intergenic 25.0%
! TTTGAATCTTTAACATCGAT+TGG + chr1.1:65559570-65559589 MS.gene21543:intron 25.0%
!! AAATTCAGGACTTTTCAATA+GGG + chr1.1:65560312-65560331 MS.gene21543:CDS 25.0%
!! AATGAAATGCATAGTTGATA+TGG + chr1.1:65560082-65560101 MS.gene21543:intron 25.0%
!! TTGAATCTTTAACATCGATT+GGG + chr1.1:65559571-65559590 MS.gene21543:intron 25.0%
!!! TGTTTTTGTTATCCTTTTCT+GGG + chr1.1:65559648-65559667 MS.gene21543:intron 25.0%
!!! TTATTGTTTCTGTAAACTAC+AGG + chr1.1:65560518-65560537 MS.gene21543:CDS 25.0%
!!! TTGTTTTTGTTATCCTTTTC+TGG + chr1.1:65559647-65559666 MS.gene21543:intron 25.0%
!!! TTTTGTGTGTTTTTGATGTT+GGG + chr1.1:65559525-65559544 MS.gene21543:intron 25.0%
!!! TTTTTGTGTGTTTTTGATGT+TGG + chr1.1:65559524-65559543 MS.gene21543:intron 25.0%
AGTCAACCTTAATGAATCTA+AGG + chr1.1:65559399-65559418 MS.gene21543:CDS 30.0%
ATTGTAAGCAAGCTTGAAAT+TGG - chr1.1:65560176-65560195 None:intergenic 30.0%
GTGCATAATTCATATGGATA+AGG + chr1.1:65559983-65560002 MS.gene21543:intron 30.0%
TGTCAAACTTCTTTACCTAA+AGG + chr1.1:65561020-65561039 MS.gene21543:CDS 30.0%
TTATCAACAAAACCCAGAAA+AGG - chr1.1:65559663-65559682 None:intergenic 30.0%
! GAAATTCAGGACTTTTCAAT+AGG + chr1.1:65560311-65560330 MS.gene21543:CDS 30.0%
! GGATCATAAAAAGGAAGTAT+AGG - chr1.1:65559867-65559886 None:intergenic 30.0%
AAAGAGAATACTCCATCTAC+AGG - chr1.1:65560277-65560296 None:intergenic 35.0%
AAGGAAGTAGAAACCAATAG+TGG - chr1.1:65561049-65561068 None:intergenic 35.0%
ACAAACCCCAAAAACTACAT+AGG - chr1.1:65559694-65559713 None:intergenic 35.0%
ACCTTTCTCAAAGATTTGTG+AGG + chr1.1:65559831-65559850 MS.gene21543:intron 35.0%
AGATTGGCAATTGGAATATG+TGG + chr1.1:65560972-65560991 MS.gene21543:CDS 35.0%
CAAACCCCAAAAACTACATA+GGG - chr1.1:65559693-65559712 None:intergenic 35.0%
CATGGAAGAAGATTATGACA+GGG - chr1.1:65560417-65560436 None:intergenic 35.0%
CTCTAAATTGGGTCAAATCA+AGG + chr1.1:65560590-65560609 MS.gene21543:CDS 35.0%
CTCTCTATTGAATGTTCCAT+CGG - chr1.1:65560212-65560231 None:intergenic 35.0%
GATCATGTCGATAGAAATTC+AGG + chr1.1:65560298-65560317 MS.gene21543:CDS 35.0%
GGAAGGATTGCATATTTGTT+GGG + chr1.1:65559592-65559611 MS.gene21543:intron 35.0%
GGTAAAGAAGTTTGACATCT+TGG - chr1.1:65561017-65561036 None:intergenic 35.0%
GTTTACTTGTTCTGGTAAGT+TGG + chr1.1:65559765-65559784 MS.gene21543:intron 35.0%
GTTTGGATCTTCTACAAGAT+TGG + chr1.1:65560956-65560975 MS.gene21543:CDS 35.0%
TCCAGAGAATGTTACTACTT+GGG + chr1.1:65560348-65560367 MS.gene21543:CDS 35.0%
TCCATCTACAGGAATTGTAT+TGG - chr1.1:65560266-65560285 None:intergenic 35.0%
TCTTCTACAAGATTGGCAAT+TGG + chr1.1:65560963-65560982 MS.gene21543:CDS 35.0%
TGACATCTTGGTTAGATTTC+TGG - chr1.1:65561005-65561024 None:intergenic 35.0%
TGTAATCCATTTGGTCCTAA+AGG + chr1.1:65560883-65560902 MS.gene21543:CDS 35.0%
TGTTGTTCCTCTCAATACTT+GGG + chr1.1:65560141-65560160 MS.gene21543:CDS 35.0%
! AATGCATAGTTGATATGGAC+AGG + chr1.1:65560087-65560106 MS.gene21543:intron 35.0%
! ACCTCACAAATCTTTGAGAA+AGG - chr1.1:65559835-65559854 None:intergenic 35.0%
! ATCTTTAACATCGATTGGGA+AGG + chr1.1:65559575-65559594 MS.gene21543:intron 35.0%
! ATGCATAGTTGATATGGACA+GGG + chr1.1:65560088-65560107 MS.gene21543:intron 35.0%
! ATGGTCCCTATGTAGTTTTT+GGG + chr1.1:65559685-65559704 MS.gene21543:intron 35.0%
! ATGGTTGGAATGCTCTAAAT+TGG + chr1.1:65560578-65560597 MS.gene21543:CDS 35.0%
! CACAAATCTTTGAGAAAGGT+GGG - chr1.1:65559831-65559850 None:intergenic 35.0%
! TCACAAATCTTTGAGAAAGG+TGG - chr1.1:65559832-65559851 None:intergenic 35.0%
! TGGTTGGAATGCTCTAAATT+GGG + chr1.1:65560579-65560598 MS.gene21543:CDS 35.0%
!! GTGTTTTTGATGTTGGGTTT+TGG + chr1.1:65559531-65559550 MS.gene21543:intron 35.0%
!!! CTGGGTTTTGTTGATAATGA+TGG + chr1.1:65559666-65559685 MS.gene21543:intron 35.0%
!!! CTTTTACCTTTAGGACCAAA+TGG - chr1.1:65560892-65560911 None:intergenic 35.0%
!!! TGGATGGAAAGTATTTTGTG+AGG + chr1.1:65560794-65560813 MS.gene21543:CDS 35.0%
AACAGAATCGGAGAAGAAAC+TGG + chr1.1:65560774-65560793 MS.gene21543:CDS 40.0%
AACCAATAGTGGCTTCCTTT+AGG - chr1.1:65561038-65561057 None:intergenic 40.0%
AAGGTTTATGCTTACATGGC+TGG + chr1.1:65560640-65560659 MS.gene21543:CDS 40.0%
AATGAGAGAAACTTCCACCA+TGG - chr1.1:65560435-65560454 None:intergenic 40.0%
ACAATCTCTGTTGTGCTCAA+GGG - chr1.1:65560394-65560413 None:intergenic 40.0%
ACCAGAACAAGTAAACCAAG+AGG - chr1.1:65559761-65559780 None:intergenic 40.0%
AGCCATGTAAGCATAAACCT+TGG - chr1.1:65560641-65560660 None:intergenic 40.0%
AGGCGTTTATCTCATTCATG+AGG - chr1.1:65561095-65561114 None:intergenic 40.0%
ATAGATAGCACTATTAGCCG+AGG - chr1.1:65560458-65560477 None:intergenic 40.0%
ATTCTTCTCCATCCACTCTT+TGG + chr1.1:65560739-65560758 MS.gene21543:CDS 40.0%
ATTGGGTCAAATCAAGGACA+TGG + chr1.1:65560596-65560615 MS.gene21543:CDS 40.0%
CATCCTGCTTGTAATCCATT+TGG + chr1.1:65560874-65560893 MS.gene21543:CDS 40.0%
CCAGAGAATGTTACTACTTG+GGG + chr1.1:65560349-65560368 MS.gene21543:CDS 40.0%
CCATGGAAGAAGATTATGAC+AGG - chr1.1:65560418-65560437 None:intergenic 40.0%
CCTGTCATAATCTTCTTCCA+TGG + chr1.1:65560415-65560434 MS.gene21543:CDS 40.0%
CTCCAAGGTTTATGCTTACA+TGG + chr1.1:65560636-65560655 MS.gene21543:CDS 40.0%
CTCCAGAGAATGTTACTACT+TGG + chr1.1:65560347-65560366 MS.gene21543:CDS 40.0%
GCCAATACAATTCCTGTAGA+TGG + chr1.1:65560262-65560281 MS.gene21543:CDS 40.0%
GGGAAGGATTGCATATTTGT+TGG + chr1.1:65559591-65559610 MS.gene21543:intron 40.0%
GTCATAATCTTCTTCCATGG+TGG + chr1.1:65560418-65560437 MS.gene21543:CDS 40.0%
TACCTAAAGGAAGCCACTAT+TGG + chr1.1:65561033-65561052 MS.gene21543:CDS 40.0%
TAGTACCTCAACATCTTCCT+CGG - chr1.1:65560713-65560732 None:intergenic 40.0%
TGCAAGATCGTGATTGGTAT+TGG + chr1.1:65560818-65560837 MS.gene21543:CDS 40.0%
TGGAAGTTTCTCTCATTCCT+CGG + chr1.1:65560438-65560457 MS.gene21543:CDS 40.0%
TGGCAATTGGAATATGTGGA+AGG + chr1.1:65560976-65560995 MS.gene21543:CDS 40.0%
TTTCTTACATGTAGGCCTCT+TGG + chr1.1:65559743-65559762 MS.gene21543:intron 40.0%
! CCACACAAACAAGGCTTTTA+GGG - chr1.1:65560933-65560952 None:intergenic 40.0%
! CCTAAAGGTAAAAGCCTTGT+AGG + chr1.1:65560898-65560917 MS.gene21543:CDS 40.0%
! CCTACAAGGCTTTTACCTTT+AGG - chr1.1:65560901-65560920 None:intergenic 40.0%
! GATGGTCCCTATGTAGTTTT+TGG + chr1.1:65559684-65559703 MS.gene21543:intron 40.0%
! GTGTTGTTCCTCTCAATACT+TGG + chr1.1:65560140-65560159 MS.gene21543:CDS 40.0%
! TCTACAGGAATTGTATTGGC+TGG - chr1.1:65560262-65560281 None:intergenic 40.0%
! TGGTCCCTATGTAGTTTTTG+GGG + chr1.1:65559686-65559705 MS.gene21543:intron 40.0%
!! ATAAGCACCCAAGTATTGAG+AGG - chr1.1:65560151-65560170 None:intergenic 40.0%
!! CTACAGGAATTGTATTGGCT+GGG - chr1.1:65560261-65560280 None:intergenic 40.0%
!! GGTGGGCAATTTTTCAACTT+TGG - chr1.1:65559814-65559833 None:intergenic 40.0%
CAACATTGCTCATCACGTTG+CGG + chr1.1:65560678-65560697 MS.gene21543:CDS 45.0%
CAACCATCTTCGTAAGCACA+CGG - chr1.1:65560565-65560584 None:intergenic 45.0%
CCCCAAGTAGTAACATTCTC+TGG - chr1.1:65560352-65560371 None:intergenic 45.0%
GAATCGGAGAAGAAACTGGA+TGG + chr1.1:65560778-65560797 MS.gene21543:CDS 45.0%
GACAATCTCTGTTGTGCTCA+AGG - chr1.1:65560395-65560414 None:intergenic 45.0%
GACATGGCTTCAAAGTGGAA+AGG + chr1.1:65560612-65560631 MS.gene21543:CDS 45.0%
GCCTCTTGGTTTACTTGTTC+TGG + chr1.1:65559757-65559776 MS.gene21543:intron 45.0%
TACATGGCTGGTGATAGTTC+CGG + chr1.1:65560652-65560671 MS.gene21543:CDS 45.0%
TAGGGAACTTGAGTCCTACA+AGG - chr1.1:65560915-65560934 None:intergenic 45.0%
TATCCGTGTGCTTACGAAGA+TGG + chr1.1:65560559-65560578 MS.gene21543:CDS 45.0%
TCAAAGTGGAAAGGACTCCA+AGG + chr1.1:65560621-65560640 MS.gene21543:CDS 45.0%
TCAAGGACATGGCTTCAAAG+TGG + chr1.1:65560607-65560626 MS.gene21543:CDS 45.0%
TCAGCAGTTAGGATGCACAA+AGG - chr1.1:65561115-65561134 None:intergenic 45.0%
TGAGGTTGCAAGATCGTGAT+TGG + chr1.1:65560812-65560831 MS.gene21543:CDS 45.0%
TGATCTCTATCTTCCCCTTC+AGG - chr1.1:65560856-65560875 None:intergenic 45.0%
TTCTCCATCCACTCTTTGGT+GGG + chr1.1:65560743-65560762 MS.gene21543:CDS 45.0%
! GAGGAAGATGTTGAGGTACT+AGG + chr1.1:65560712-65560731 MS.gene21543:CDS 45.0%
! GCCACACAAACAAGGCTTTT+AGG - chr1.1:65560934-65560953 None:intergenic 45.0%
!! AAAAGCCTTGTTTGTGTGGC+TGG + chr1.1:65560934-65560953 MS.gene21543:CDS 45.0%
!! ATGTTGGGTTTTGGCGTTGA+AGG + chr1.1:65559540-65559559 MS.gene21543:intron 45.0%
!! CCCTAAAAGCCTTGTTTGTG+TGG + chr1.1:65560930-65560949 MS.gene21543:CDS 45.0%
!! GGACCAAATGGATTACAAGC+AGG - chr1.1:65560880-65560899 None:intergenic 45.0%
!! GTAGTAACATTCTCTGGAGC+AGG - chr1.1:65560346-65560365 None:intergenic 45.0%
!! TGTTGGGTTTTGGCGTTGAA+GGG + chr1.1:65559541-65559560 MS.gene21543:intron 45.0%
AATCTACTAAGACGCGCCGA+TGG + chr1.1:65560193-65560212 MS.gene21543:CDS 50.0%
AGCTGAGTTTCTTGACCGCA+AGG + chr1.1:65560234-65560253 MS.gene21543:CDS 50.0%
CAGCCTTACACATGCTCACA+AGG - chr1.1:65560498-65560517 None:intergenic 50.0%
CCAAACCAGCCACACAAACA+AGG - chr1.1:65560942-65560961 None:intergenic 50.0%
CGTGTGCTTACGAAGATGGT+TGG + chr1.1:65560563-65560582 MS.gene21543:CDS 50.0%
CTTCTCCATCCACTCTTTGG+TGG + chr1.1:65560742-65560761 MS.gene21543:CDS 50.0%
GTGATGAGCAATGTTGCCTC+CGG - chr1.1:65560674-65560693 None:intergenic 50.0%
TACACATGCTCACAAGGCGA+CGG - chr1.1:65560492-65560511 None:intergenic 50.0%
TCGCCTTGTGAGCATGTGTA+AGG + chr1.1:65560492-65560511 MS.gene21543:CDS 50.0%
TCTCCATCCACTCTTTGGTG+GGG + chr1.1:65560744-65560763 MS.gene21543:CDS 50.0%
TGGGGAGAAGCGAACAGAAT+CGG + chr1.1:65560762-65560781 MS.gene21543:CDS 50.0%
! CCTTGTTTGTGTGGCTGGTT+TGG + chr1.1:65560939-65560958 MS.gene21543:CDS 50.0%
! GCGAGCTTTTCTACCTGAAG+GGG + chr1.1:65560840-65560859 MS.gene21543:CDS 50.0%
! GGCGAGCTTTTCTACCTGAA+GGG + chr1.1:65560839-65560858 MS.gene21543:CDS 50.0%
! TGGCGAGCTTTTCTACCTGA+AGG + chr1.1:65560838-65560857 MS.gene21543:CDS 50.0%
!! GTTGGGTTTTGGCGTTGAAG+GGG + chr1.1:65559542-65559561 MS.gene21543:intron 50.0%
!! TTGTATTGGCTGGGACCTTG+CGG - chr1.1:65560252-65560271 None:intergenic 50.0%
ACAGGAGATCACCCGAGCAT+CGG + chr1.1:65560536-65560555 MS.gene21543:CDS 55.0%
AGCACACGGATACCGATGCT+CGG - chr1.1:65560551-65560570 None:intergenic 55.0%
AGCTGCCGAGGAAGATGTTG+AGG + chr1.1:65560705-65560724 MS.gene21543:CDS 55.0%
ATGGCTGGTGATAGTTCCGG+AGG + chr1.1:65560655-65560674 MS.gene21543:CDS 55.0%
TCTCCCCACCAAAGAGTGGA+TGG - chr1.1:65560750-65560769 None:intergenic 55.0%
CGCTTCTCCCCACCAAAGAG+TGG - chr1.1:65560754-65560773 None:intergenic 60.0%
GCACACGGATACCGATGCTC+GGG - chr1.1:65560550-65560569 None:intergenic 60.0%
CGTTGCGGTGAGAGCTGCCG+AGG + chr1.1:65560693-65560712 MS.gene21543:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 65559382 65561134 65559382 ID=MS.gene21543
chr1.1 mRNA 65559382 65561134 65559382 ID=MS.gene21543.t1;Parent=MS.gene21543
chr1.1 exon 65559382 65559420 65559382 ID=MS.gene21543.t1.exon1;Parent=MS.gene21543.t1
chr1.1 CDS 65559382 65559420 65559382 ID=cds.MS.gene21543.t1;Parent=MS.gene21543.t1
chr1.1 exon 65560139 65561134 65560139 ID=MS.gene21543.t1.exon2;Parent=MS.gene21543.t1
chr1.1 CDS 65560139 65561134 65560139 ID=cds.MS.gene21543.t1;Parent=MS.gene21543.t1
Gene Sequence

>MS.gene21543

ATGGCTGGAAGTAATGAAGTCAACCTTAATGAATCTAAGGTAACAACTTTCTTTGTTTTTTTTGGTCTTTTTCTTCTTATTTTATTTGATTATAGCATTGTGTATTTTATGTATTCCTTCTTTAATTTGTTTCTAGAAGTTTTTTTTGTGTGTTTTTGATGTTGGGTTTTGGCGTTGAAGGGGAAAAGTTTGAATCTTTAACATCGATTGGGAAGGATTGCATATTTGTTGGGAACATCTTTGAGTTAAAGAGAGTGTTGTTTCTTTGTTTTTGTTATCCTTTTCTGGGTTTTGTTGATAATGATGGTCCCTATGTAGTTTTTGGGGTTTGTTTCAATTTTTTGTTTTGTACTTTCTATTTTTTCTTACATGTAGGCCTCTTGGTTTACTTGTTCTGGTAAGTTGGTAAATTTCTGTTTGATTTACTCTCCAAAGTTGAAAAATTGCCCACCTTTCTCAAAGATTTGTGAGGTTCTTTCACGCCTATACTTCCTTTTTATGATCCTTCTTTTCAATAATTTCTTGCTTTTCTAAAGGGATGAGCTTTGATTAGATGATTTTATTCTTCTTTTGAAGTATTAGTACTATAGTGTCAAATGAAGTGCATAATTCATATGGATAAGGTTCTAATAACATAGTTCATATCCATAAGCTTTGATTAGAGAATATTTTTTCTTTCTTGTTAAGTACTGTTGTGCCAAATGAAATGCATAGTTGATATGGACAGGGTTCTAATAACATGTTACAATCTTTGTAGAGTGTTGTTCCTCTCAATACTTGGGTGCTTATCTCCAATTTCAAGCTTGCTTACAATCTACTAAGACGCGCCGATGGAACATTCAATAGAGAGTTAGCTGAGTTTCTTGACCGCAAGGTCCCAGCCAATACAATTCCTGTAGATGGAGTATTCTCTTTCGATCATGTCGATAGAAATTCAGGACTTTTCAATAGGGTTTATCAACCTGCTCCAGAGAATGTTACTACTTGGGGAATTATAGAGCTAGAAAAGCCCTTGAGCACAACAGAGATTGTCCCTGTCATAATCTTCTTCCATGGTGGAAGTTTCTCTCATTCCTCGGCTAATAGTGCTATCTATGACACTTTCTGCCGTCGCCTTGTGAGCATGTGTAAGGCTGTTATTGTTTCTGTAAACTACAGGAGATCACCCGAGCATCGGTATCCGTGTGCTTACGAAGATGGTTGGAATGCTCTAAATTGGGTCAAATCAAGGACATGGCTTCAAAGTGGAAAGGACTCCAAGGTTTATGCTTACATGGCTGGTGATAGTTCCGGAGGCAACATTGCTCATCACGTTGCGGTGAGAGCTGCCGAGGAAGATGTTGAGGTACTAGGTAACATTCTTCTCCATCCACTCTTTGGTGGGGAGAAGCGAACAGAATCGGAGAAGAAACTGGATGGAAAGTATTTTGTGAGGTTGCAAGATCGTGATTGGTATTGGCGAGCTTTTCTACCTGAAGGGGAAGATAGAGATCATCCTGCTTGTAATCCATTTGGTCCTAAAGGTAAAAGCCTTGTAGGACTCAAGTTCCCTAAAAGCCTTGTTTGTGTGGCTGGTTTGGATCTTCTACAAGATTGGCAATTGGAATATGTGGAAGGTCTCCAGAAATCTAACCAAGATGTCAAACTTCTTTACCTAAAGGAAGCCACTATTGGTTTCTACTTCCTTCCTAATAATGATCATTTCTATTGCCTCATGAATGAGATAAACGCCTTTGTGCATCCTAACTGCTGA
Protein sequence

>MS.gene21543.t1

MAGSNEVNLNESKSVVPLNTWVLISNFKLAYNLLRRADGTFNRELAEFLDRKVPANTIPVDGVFSFDHVDRNSGLFNRVYQPAPENVTTWGIIELEKPLSTTEIVPVIIFFHGGSFSHSSANSAIYDTFCRRLVSMCKAVIVSVNYRRSPEHRYPCAYEDGWNALNWVKSRTWLQSGKDSKVYAYMAGDSSGGNIAHHVAVRAAEEDVEVLGNILLHPLFGGEKRTESEKKLDGKYFVRLQDRDWYWRAFLPEGEDRDHPACNPFGPKGKSLVGLKFPKSLVCVAGLDLLQDWQLEYVEGLQKSNQDVKLLYLKEATIGFYFLPNNDHFYCLMNEINAFVHPNC