AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062658

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062658.t1 MTR_6g065340 86.610 351 31 1 1 351 1 335 0.0 605
MS.gene062658.t1 MTR_6g065270 82.789 337 54 1 19 351 6 342 0.0 589
MS.gene062658.t1 MTR_6g065370 78.466 339 73 0 13 351 2 340 0.0 570
MS.gene062658.t1 MTR_6g065400 77.581 339 76 0 13 351 2 340 0.0 565
MS.gene062658.t1 MTR_6g065120 77.419 341 76 1 12 351 2 342 0.0 558
MS.gene062658.t1 MTR_6g065110 67.273 330 108 0 16 345 6 335 6.49e-174 487
MS.gene062658.t1 MTR_6g065430 58.308 331 129 2 18 346 6 329 1.05e-139 400
MS.gene062658.t1 MTR_1g082210 36.919 344 208 3 8 345 1 341 7.73e-75 235
MS.gene062658.t1 MTR_7g093950 36.857 350 206 6 8 345 1 347 5.19e-74 233
MS.gene062658.t1 MTR_1g089310 38.040 347 202 6 7 345 16 357 2.35e-73 231
MS.gene062658.t1 MTR_1g089310 37.861 346 202 6 8 345 1 341 9.83e-73 229
MS.gene062658.t1 MTR_6g464280 35.484 310 185 8 43 345 24 325 5.20e-51 172
MS.gene062658.t1 MTR_8g035520 32.641 337 201 9 22 347 6 327 1.34e-46 161
MS.gene062658.t1 MTR_8g035540 34.076 314 186 6 42 344 13 316 1.47e-46 161
MS.gene062658.t1 MTR_7g406880 32.013 303 179 7 66 346 54 351 2.68e-45 158
MS.gene062658.t1 MTR_6g464510 33.762 311 189 7 42 345 27 327 3.11e-45 157
MS.gene062658.t1 MTR_1g102160 33.861 316 183 9 42 344 23 325 2.98e-44 155
MS.gene062658.t1 MTR_4g050140 32.281 285 175 4 71 346 42 317 3.42e-43 152
MS.gene062658.t1 MTR_0007s0520 33.444 302 173 10 59 346 39 326 3.51e-42 149
MS.gene062658.t1 MTR_6g464320 34.601 263 159 5 58 312 37 294 4.55e-42 149
MS.gene062658.t1 MTR_8g035500 31.410 312 193 7 43 344 17 317 5.89e-42 149
MS.gene062658.t1 MTR_1g102170 36.946 203 111 4 70 267 69 259 1.65e-40 145
MS.gene062658.t1 MTR_1g111280 32.680 306 177 9 59 349 52 343 3.27e-40 145
MS.gene062658.t1 MTR_7g107040 31.741 293 179 5 66 348 39 320 1.79e-39 142
MS.gene062658.t1 MTR_6g464120 36.019 211 125 4 60 267 43 246 8.26e-38 142
MS.gene062658.t1 MTR_1g012430 30.645 310 195 8 42 346 15 309 1.32e-37 137
MS.gene062658.t1 MTR_4g086540 30.844 308 173 7 49 348 33 308 1.28e-36 134
MS.gene062658.t1 MTR_3g012440 30.599 317 190 5 52 346 34 342 2.60e-36 134
MS.gene062658.t1 MTR_3g101420 30.667 300 178 7 60 346 34 316 2.05e-34 129
MS.gene062658.t1 MTR_4g086510 30.847 295 184 6 60 345 35 318 2.69e-34 129
MS.gene062658.t1 MTR_4g086480 31.293 294 179 8 60 344 39 318 1.39e-33 127
MS.gene062658.t1 MTR_7g056207 29.831 295 187 6 60 345 67 350 2.23e-31 121
MS.gene062658.t1 MTR_4g086470 30.743 296 182 7 60 346 38 319 6.92e-30 117
MS.gene062658.t1 MTR_6g022710 28.374 289 153 6 96 335 142 425 7.29e-28 113
MS.gene062658.t1 MTR_3g450680 29.054 296 172 8 64 339 48 325 2.19e-27 110
MS.gene062658.t1 MTR_1g105020 28.378 296 184 8 64 344 40 322 2.74e-27 110
MS.gene062658.t1 MTR_5g005850 27.812 320 196 11 43 344 26 328 9.24e-25 103
MS.gene062658.t1 MTR_8g040860 26.667 300 190 8 57 339 36 322 1.75e-24 102
MS.gene062658.t1 MTR_5g005900 27.551 294 188 7 68 346 45 328 1.95e-23 99.4
MS.gene062658.t1 MTR_5g005920 27.660 329 206 12 33 345 16 328 4.53e-23 98.6
MS.gene062658.t1 MTR_6g065470 34.503 171 80 3 18 170 6 162 1.33e-21 90.5
MS.gene062658.t1 MTR_7g051020 29.667 300 189 7 59 346 38 327 4.61e-21 92.8
MS.gene062658.t1 MTR_1g104930 30.068 296 169 9 70 346 48 324 3.18e-19 87.4
MS.gene062658.t1 MTR_1g104933 29.210 291 174 8 70 346 46 318 2.30e-16 79.3
MS.gene062658.t1 MTR_0007s0480 30.726 179 103 8 182 346 1 172 1.24e-14 71.6
MS.gene062658.t1 MTR_6g065480 53.448 58 26 1 244 301 1 57 1.40e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062658.t1 AT5G23530 54.360 344 139 4 11 350 3 332 3.02e-138 396
MS.gene062658.t1 AT5G27320 37.026 343 208 3 8 345 1 340 7.59e-78 243
MS.gene062658.t1 AT3G05120 34.783 345 215 3 8 345 1 342 7.89e-71 224
MS.gene062658.t1 AT3G63010 36.337 344 207 6 15 351 10 348 2.05e-69 221
MS.gene062658.t1 AT5G62180 34.185 313 191 9 43 347 21 326 3.64e-50 171
MS.gene062658.t1 AT2G45600 31.973 294 176 6 70 350 39 321 2.28e-44 155
MS.gene062658.t1 AT5G06570 36.897 290 161 6 71 348 65 344 1.04e-43 154
MS.gene062658.t1 AT5G06570 36.897 290 161 6 71 348 65 344 1.04e-43 154
MS.gene062658.t1 AT5G06570 36.897 290 161 6 71 348 50 329 1.06e-43 154
MS.gene062658.t1 AT5G06570 36.897 290 161 6 71 348 50 329 1.06e-43 154
MS.gene062658.t1 AT5G16080 32.857 280 164 6 59 327 54 320 2.27e-42 150
MS.gene062658.t1 AT1G47480 31.515 330 202 10 24 349 5 314 1.55e-41 147
MS.gene062658.t1 AT1G68620 31.944 288 173 8 67 345 57 330 1.81e-41 148
MS.gene062658.t1 AT2G45610 30.892 314 195 7 43 345 22 324 1.18e-40 145
MS.gene062658.t1 AT2G03550 31.879 298 175 8 60 346 32 312 3.04e-33 125
MS.gene062658.t1 AT1G19190 31.034 319 184 11 43 346 18 315 9.10e-33 124
MS.gene062658.t1 AT3G48700 29.032 310 179 8 60 344 32 325 4.99e-32 122
MS.gene062658.t1 AT3G27320 27.171 357 180 7 58 335 48 403 3.48e-29 117
MS.gene062658.t1 AT5G14310 29.720 286 152 6 96 335 139 421 7.24e-29 116
MS.gene062658.t1 AT3G48690 30.798 263 161 6 60 311 32 284 1.15e-27 110
MS.gene062658.t1 AT3G27320 29.252 294 158 7 83 335 151 435 2.81e-27 112
MS.gene062658.t1 AT1G49640 34.066 182 111 4 48 228 23 196 1.37e-25 105
MS.gene062658.t1 AT1G49660 29.412 255 158 7 64 308 36 278 1.69e-25 105
MS.gene062658.t1 AT1G49650 30.847 295 183 10 64 349 92 374 1.47e-23 100

Find 105 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACGGGAAGGGAGGTTATTT+TGG 0.215751 6.4:-46110380 None:intergenic
CGCGGGGCTTCCAAGGAATA+AGG 0.269212 6.4:-46110135 None:intergenic
AGACAGTGCCGGTGCAAATT+TGG 0.273184 6.4:+46110645 MS.gene062658:CDS
TGCGGAGGATTTGTCGGGTT+TGG 0.280062 6.4:+46110894 MS.gene062658:CDS
GGGGTTAACGGTAATGTCTT+TGG 0.321933 6.4:-46110287 None:intergenic
GTTGAAGAAATGTGGGAAAA+AGG 0.337242 6.4:+46110990 MS.gene062658:CDS
TGGCACATCACCTTGCAGTT+AGG 0.348786 6.4:+46110665 MS.gene062658:CDS
CAACAACAGCGGAGATTTGC+TGG 0.356181 6.4:-46110483 None:intergenic
GGTTTGATCCTTTAAATGAT+TGG 0.359729 6.4:+46110947 MS.gene062658:CDS
CTTCCAAGGAATAAGGGGTT+TGG 0.365944 6.4:-46110128 None:intergenic
GGCAAAAGAGGTATTATGAT+TGG 0.376841 6.4:+46110968 MS.gene062658:CDS
GGCACATCACCTTGCAGTTA+GGG 0.384459 6.4:+46110666 MS.gene062658:CDS
TTCCAAGGAATAAGGGGTTT+GGG 0.393433 6.4:-46110127 None:intergenic
GGCGGAGAAGTCCTCGGATA+TGG 0.399898 6.4:+46110349 MS.gene062658:CDS
CCGTTAACGGGAGTGGCATT+AGG 0.400101 6.4:-46110256 None:intergenic
TCGATACCAACCCGATAATC+CGG 0.408280 6.4:-46110714 None:intergenic
GCCGAATGCGGAGGATTTGT+CGG 0.409294 6.4:+46110888 MS.gene062658:CDS
AACCCAAACCCCTTATTCCT+TGG 0.419489 6.4:+46110125 MS.gene062658:CDS
CTCCGGGAAATCCGGATTAT+CGG 0.421000 6.4:+46110703 MS.gene062658:CDS
TTAACCCCGAAAATAATGTT+TGG 0.422380 6.4:+46110302 MS.gene062658:CDS
GTGTCAAAATGTTTCATTGC+TGG 0.422545 6.4:+46110622 MS.gene062658:CDS
ACCCGATAATCCGGATTTCC+CGG 0.425872 6.4:-46110705 None:intergenic
GCGGGGCTTCCAAGGAATAA+GGG 0.428492 6.4:-46110134 None:intergenic
TTTGGTGGAAACGCCGTTAA+CGG 0.430126 6.4:-46110269 None:intergenic
CCTAATGCCACTCCCGTTAA+CGG 0.433533 6.4:+46110256 MS.gene062658:CDS
CCGAATGCGGAGGATTTGTC+GGG 0.438724 6.4:+46110889 MS.gene062658:CDS
AAGCAGCGCTCCGGGAAATC+CGG 0.439201 6.4:+46110695 MS.gene062658:CDS
CTCTTTACACCCTCTGTTGG+CGG 0.440539 6.4:+46110331 MS.gene062658:CDS
CCCGACAAATCCTCCGCATT+CGG 0.445071 6.4:-46110889 None:intergenic
GGGTTTGTAAAGCAGCGCTC+CGG 0.454316 6.4:+46110686 MS.gene062658:CDS
TTGGTGGAAACGCCGTTAAC+GGG 0.454826 6.4:-46110268 None:intergenic
TGGCATTAGGTGAGGATTTG+CGG 0.456562 6.4:-46110243 None:intergenic
CTTTAAATGATTGGCAAAAG+AGG 0.459493 6.4:+46110956 MS.gene062658:CDS
TCCGGGAAATCCGGATTATC+GGG 0.462547 6.4:+46110704 MS.gene062658:CDS
AATAATCAATCATTTAGAAC+CGG 0.473533 6.4:-46111121 None:intergenic
TGAGCAGATTCAAGCAAGTC+AGG 0.473765 6.4:-46111060 None:intergenic
ATATTTGGATGAGAATAAAA+CGG 0.477006 6.4:+46110579 MS.gene062658:CDS
GAGGCGGAGATTAAACTTGT+TGG 0.477896 6.4:+46110769 MS.gene062658:CDS
GAGGGATTGAACCGGGATCA+TGG 0.494441 6.4:+46110847 MS.gene062658:CDS
GGTTTGTAAAGCAGCGCTCC+GGG 0.495981 6.4:+46110687 MS.gene062658:CDS
TATGATTGGTTGAAGAAATG+TGG 0.503360 6.4:+46110982 MS.gene062658:CDS
ATGGTAGCGCGGGGCTTCCA+AGG 0.509543 6.4:-46110142 None:intergenic
CGTCTCTTTACACCCTCTGT+TGG 0.511038 6.4:+46110328 MS.gene062658:CDS
GGAAACGCCGTTAACGGGAG+TGG 0.513035 6.4:-46110263 None:intergenic
AGATTTGCTGGCAGAGTCTA+CGG 0.515690 6.4:-46110471 None:intergenic
GAGTTTGTCAACAAAGTCTC+CGG 0.518446 6.4:+46111102 MS.gene062658:CDS
CGATGGTGAGGACGGATTGG+TGG 0.520340 6.4:+46110806 MS.gene062658:CDS
TGGTGAGGACGGATTGGTGG+TGG 0.527652 6.4:+46110809 MS.gene062658:CDS
GAGGACGGATTGGTGGTGGA+AGG 0.528358 6.4:+46110813 MS.gene062658:CDS
TGTCGATGGTGAGGACGGAT+TGG 0.534568 6.4:+46110803 MS.gene062658:CDS
AAGAAGTGAAATGGTAGCGC+GGG 0.536287 6.4:-46110152 None:intergenic
GAAGAAAATGACGACGGGAA+GGG 0.539782 6.4:-46110392 None:intergenic
GTTAACGGTAATGTCTTTGG+TGG 0.540475 6.4:-46110284 None:intergenic
GGAAGAAAATGACGACGGGA+AGG 0.541051 6.4:-46110393 None:intergenic
CATTAACAGCACCATGATCC+CGG 0.544471 6.4:-46110858 None:intergenic
TGGATCGCCGTTGGTGTCGA+TGG 0.547626 6.4:+46110789 MS.gene062658:CDS
CGCCGTTGGTGTCGATGGTG+AGG 0.551306 6.4:+46110794 MS.gene062658:CDS
ATGATTGGTTGAAGAAATGT+GGG 0.557528 6.4:+46110983 MS.gene062658:CDS
TTTACCGGAGGGATTGAACC+GGG 0.557831 6.4:+46110840 MS.gene062658:CDS
TGCGACGGATAGTGATGCTC+CGG 0.557947 6.4:-46110529 None:intergenic
AAAAGCATCATAGTAGATTG+AGG 0.560272 6.4:-46110446 None:intergenic
TGGTGGGGAGGAACGGACTG+AGG 0.564345 6.4:+46110750 MS.gene062658:CDS
TTCATTGCTGGAGACAGTGC+CGG 0.566877 6.4:+46110634 MS.gene062658:CDS
TGATCCCGGTTCAATCCCTC+CGG 0.569200 6.4:-46110844 None:intergenic
TAAACTTGTTGGATCGCCGT+TGG 0.569356 6.4:+46110780 MS.gene062658:CDS
TGTAAGCGGGCCGAATGCGG+AGG 0.569404 6.4:+46110879 MS.gene062658:CDS
GGAAATCCGGATTATCGGGT+TGG 0.574486 6.4:+46110708 MS.gene062658:CDS
CGGGGCTTCCAAGGAATAAG+GGG 0.580014 6.4:-46110133 None:intergenic
ATGGTGCTGTTAATGTAAGC+GGG 0.580275 6.4:+46110866 MS.gene062658:CDS
TCTGTTGGCGGAGAAGTCCT+CGG 0.584137 6.4:+46110343 MS.gene062658:CDS
AGGACTTCTCCGCCAACAGA+GGG 0.584418 6.4:-46110340 None:intergenic
TGGGGAGGAACGGACTGAGG+CGG 0.585654 6.4:+46110753 MS.gene062658:CDS
GATAGTGATGCTCCGGTGTG+AGG 0.586887 6.4:-46110522 None:intergenic
GCATCATAGTAGATTGAGGA+GGG 0.595871 6.4:-46110442 None:intergenic
GTTGGTGTCGATGGTGAGGA+CGG 0.597501 6.4:+46110798 MS.gene062658:CDS
CCGTGGAAGAAAATGACGAC+GGG 0.598794 6.4:-46110397 None:intergenic
AACGGGAGTGGCATTAGGTG+AGG 0.601408 6.4:-46110251 None:intergenic
CATGGTGCTGTTAATGTAAG+CGG 0.604475 6.4:+46110865 MS.gene062658:CDS
AGTGATGCTCCGGTGTGAGG+CGG 0.605919 6.4:-46110519 None:intergenic
GTCCTCACCATCGACACCAA+CGG 0.614135 6.4:-46110796 None:intergenic
GTGATGTGCCAAATTTGCAC+CGG 0.614788 6.4:-46110653 None:intergenic
CAGCAAAATCATGATAGATG+AGG 0.617404 6.4:+46110069 None:intergenic
CAACAGAGGGTGTAAAGAGA+CGG 0.619635 6.4:-46110327 None:intergenic
CAAAATCATGATAGATGAGG+AGG 0.620704 6.4:+46110072 None:intergenic
GACGCTTGTTGTCGCTCTAA+CGG 0.621428 6.4:+46110187 MS.gene062658:CDS
GAAAATGACGACGGGAAGGG+AGG 0.625225 6.4:-46110389 None:intergenic
GGAGAAGTCCTCGGATATGG+TGG 0.626550 6.4:+46110352 MS.gene062658:CDS
GAGGACTTCTCCGCCAACAG+AGG 0.633257 6.4:-46110341 None:intergenic
TCAGTTGATTATGCAAGTCA+AGG 0.633424 6.4:+46111080 MS.gene062658:CDS
TGGTTGCACCACCATATCCG+AGG 0.641388 6.4:-46110360 None:intergenic
CTTTACAAACCCTAACTGCA+AGG 0.644163 6.4:-46110675 None:intergenic
TAATGTAAGCGGGCCGAATG+CGG 0.645773 6.4:+46110876 MS.gene062658:CDS
TATCCGTCGCAATATGATGA+CGG 0.665117 6.4:+46110541 MS.gene062658:CDS
CATCATAGTAGATTGAGGAG+GGG 0.673790 6.4:-46110441 None:intergenic
AAAGAAGTGAAATGGTAGCG+CGG 0.678287 6.4:-46110153 None:intergenic
AGCATCATAGTAGATTGAGG+AGG 0.682743 6.4:-46110443 None:intergenic
TCTCCGTCATCATATTGCGA+CGG 0.686474 6.4:-46110544 None:intergenic
ACCGTGGAAGAAAATGACGA+CGG 0.693683 6.4:-46110398 None:intergenic
AGAAGTGAAATGGTAGCGCG+GGG 0.693711 6.4:-46110151 None:intergenic
TGTCAACTACCGCCTCACAC+CGG 0.695639 6.4:+46110510 MS.gene062658:CDS
TGAAGAAGTTAAAGAGACGA+CGG 0.697225 6.4:-46110219 None:intergenic
GTCGCAATATGATGACGGAG+AGG 0.697229 6.4:+46110546 MS.gene062658:CDS
GTTAGAGCGACAACAAGCGT+CGG 0.706955 6.4:-46110185 None:intergenic
GATAGTTGAAGCCACCACCG+TGG 0.715381 6.4:-46110414 None:intergenic
GTTGACAGAGACAACAACAG+CGG 0.758726 6.4:-46110494 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGGATGAGAATAAAA+CGG + chr6.4:46110579-46110598 MS.gene062658:CDS 20.0%
!! TAAAAATGAAAGAAGTGAAA+TGG - chr6.4:46110164-46110183 None:intergenic 20.0%
!!! TATATAAAAAGCATGAATCA+TGG - chr6.4:46111037-46111056 None:intergenic 20.0%
CCAATAAAAACAAGCGTATT+CGG - chr6.4:46110925-46110944 None:intergenic 30.0%
CTTTAAATGATTGGCAAAAG+AGG + chr6.4:46110956-46110975 MS.gene062658:CDS 30.0%
GGTTTGATCCTTTAAATGAT+TGG + chr6.4:46110947-46110966 MS.gene062658:CDS 30.0%
TTAACCCCGAAAATAATGTT+TGG + chr6.4:46110302-46110321 MS.gene062658:CDS 30.0%
! ACGGAACCAAACATTATTTT+CGG - chr6.4:46110311-46110330 None:intergenic 30.0%
! ATGATTGGTTGAAGAAATGT+GGG + chr6.4:46110983-46111002 MS.gene062658:CDS 30.0%
! CTCTTTTGCCAATCATTTAA+AGG - chr6.4:46110958-46110977 None:intergenic 30.0%
! TAAAAAGCATGAATCATGGT+TGG - chr6.4:46111033-46111052 None:intergenic 30.0%
! TATGATTGGTTGAAGAAATG+TGG + chr6.4:46110982-46111001 MS.gene062658:CDS 30.0%
! TGAGAATAAAACGGTTTTAC+CGG + chr6.4:46110588-46110607 MS.gene062658:CDS 30.0%
!! AAAAGCATCATAGTAGATTG+AGG - chr6.4:46110449-46110468 None:intergenic 30.0%
!! AACATTATTTTCGGGGTTAA+CGG - chr6.4:46110302-46110321 None:intergenic 30.0%
!!! AATACGCTTGTTTTTATTGG+TGG + chr6.4:46110925-46110944 MS.gene062658:CDS 30.0%
!!! AGAGGCTGTTTTGAAATATT+TGG + chr6.4:46110564-46110583 MS.gene062658:CDS 30.0%
!!! ATACGCTTGTTTTTATTGGT+GGG + chr6.4:46110926-46110945 MS.gene062658:CDS 30.0%
GTGTCAAAATGTTTCATTGC+TGG + chr6.4:46110622-46110641 MS.gene062658:CDS 35.0%
GTTGAAGAAATGTGGGAAAA+AGG + chr6.4:46110990-46111009 MS.gene062658:CDS 35.0%
TCAGTTGATTATGCAAGTCA+AGG + chr6.4:46111080-46111099 MS.gene062658:CDS 35.0%
TGAAGAAGTTAAAGAGACGA+CGG - chr6.4:46110222-46110241 None:intergenic 35.0%
! CGGAACCAAACATTATTTTC+GGG - chr6.4:46110310-46110329 None:intergenic 35.0%
! GGAACCAAACATTATTTTCG+GGG - chr6.4:46110309-46110328 None:intergenic 35.0%
! TTTGACACATCAGCATTTTC+CGG - chr6.4:46110610-46110629 None:intergenic 35.0%
!! GGCAAAAGAGGTATTATGAT+TGG + chr6.4:46110968-46110987 MS.gene062658:CDS 35.0%
!!! CCGAATACGCTTGTTTTTAT+TGG + chr6.4:46110922-46110941 MS.gene062658:CDS 35.0%
!!! GTATCGATTCAGCCTTTTTT+TGG + chr6.4:46110730-46110749 MS.gene062658:CDS 35.0%
AGCATCATAGTAGATTGAGG+AGG - chr6.4:46110446-46110465 None:intergenic 40.0%
CATCATAGTAGATTGAGGAG+GGG - chr6.4:46110444-46110463 None:intergenic 40.0%
CTTTACAAACCCTAACTGCA+AGG - chr6.4:46110678-46110697 None:intergenic 40.0%
GAGTTTGTCAACAAAGTCTC+CGG + chr6.4:46111102-46111121 MS.gene062658:CDS 40.0%
GCATCATAGTAGATTGAGGA+GGG - chr6.4:46110445-46110464 None:intergenic 40.0%
GTTAACGGTAATGTCTTTGG+TGG - chr6.4:46110287-46110306 None:intergenic 40.0%
TATCCGTCGCAATATGATGA+CGG + chr6.4:46110541-46110560 MS.gene062658:CDS 40.0%
TTCCAAGGAATAAGGGGTTT+GGG - chr6.4:46110130-46110149 None:intergenic 40.0%
! AAAGAAGTGAAATGGTAGCG+CGG - chr6.4:46110156-46110175 None:intergenic 40.0%
!! ATGGTGCTGTTAATGTAAGC+GGG + chr6.4:46110866-46110885 MS.gene062658:CDS 40.0%
!! CATGGTGCTGTTAATGTAAG+CGG + chr6.4:46110865-46110884 MS.gene062658:CDS 40.0%
!!! CGATTCAGCCTTTTTTTGGT+GGG + chr6.4:46110734-46110753 MS.gene062658:CDS 40.0%
!!! GATTCAGCCTTTTTTTGGTG+GGG + chr6.4:46110735-46110754 MS.gene062658:CDS 40.0%
!!! TCGATTCAGCCTTTTTTTGG+TGG + chr6.4:46110733-46110752 MS.gene062658:CDS 40.0%
AACCCAAACCCCTTATTCCT+TGG + chr6.4:46110125-46110144 MS.gene062658:CDS 45.0%
ACCGTGGAAGAAAATGACGA+CGG - chr6.4:46110401-46110420 None:intergenic 45.0%
CATTAACAGCACCATGATCC+CGG - chr6.4:46110861-46110880 None:intergenic 45.0%
CTTCCAAGGAATAAGGGGTT+TGG - chr6.4:46110131-46110150 None:intergenic 45.0%
GAAGAAAATGACGACGGGAA+GGG - chr6.4:46110395-46110414 None:intergenic 45.0%
GAGGCGGAGATTAAACTTGT+TGG + chr6.4:46110769-46110788 MS.gene062658:CDS 45.0%
GGGGTTAACGGTAATGTCTT+TGG - chr6.4:46110290-46110309 None:intergenic 45.0%
GTGATGTGCCAAATTTGCAC+CGG - chr6.4:46110656-46110675 None:intergenic 45.0%
GTTGACAGAGACAACAACAG+CGG - chr6.4:46110497-46110516 None:intergenic 45.0%
TCGATACCAACCCGATAATC+CGG - chr6.4:46110717-46110736 None:intergenic 45.0%
TCTCCGTCATCATATTGCGA+CGG - chr6.4:46110547-46110566 None:intergenic 45.0%
TGAGCAGATTCAAGCAAGTC+AGG - chr6.4:46111063-46111082 None:intergenic 45.0%
TGGCATTAGGTGAGGATTTG+CGG - chr6.4:46110246-46110265 None:intergenic 45.0%
TTTGGTGGAAACGCCGTTAA+CGG - chr6.4:46110272-46110291 None:intergenic 45.0%
! AAGAAGTGAAATGGTAGCGC+GGG - chr6.4:46110155-46110174 None:intergenic 45.0%
! CAACAGAGGGTGTAAAGAGA+CGG - chr6.4:46110330-46110349 None:intergenic 45.0%
! TTTTACCGGAGGGATTGAAC+CGG + chr6.4:46110839-46110858 MS.gene062658:CDS 45.0%
!! AGATTTGCTGGCAGAGTCTA+CGG - chr6.4:46110474-46110493 None:intergenic 45.0%
!! TAAACTTGTTGGATCGCCGT+TGG + chr6.4:46110780-46110799 MS.gene062658:CDS 45.0%
!!! CTTTTTTTGGTGGGGAGGAA+CGG + chr6.4:46110743-46110762 MS.gene062658:CDS 45.0%
!!! GTGGTGGAAGGTGTTTTTAC+CGG + chr6.4:46110825-46110844 MS.gene062658:CDS 45.0%
!!! TGGAAGGTGTTTTTACCGGA+GGG + chr6.4:46110829-46110848 MS.gene062658:CDS 45.0%
ACCCGATAATCCGGATTTCC+CGG - chr6.4:46110708-46110727 None:intergenic 50.0%
CAACAACAGCGGAGATTTGC+TGG - chr6.4:46110486-46110505 None:intergenic 50.0%
CCGTGGAAGAAAATGACGAC+GGG - chr6.4:46110400-46110419 None:intergenic 50.0%
CCTAATGCCACTCCCGTTAA+CGG + chr6.4:46110256-46110275 MS.gene062658:CDS 50.0%
CGTCTCTTTACACCCTCTGT+TGG + chr6.4:46110328-46110347 MS.gene062658:CDS 50.0%
CGTTCCTCCCCACCAAAAAA+AGG - chr6.4:46110745-46110764 None:intergenic 50.0%
CTCCGGGAAATCCGGATTAT+CGG + chr6.4:46110703-46110722 MS.gene062658:CDS 50.0%
CTCTTTACACCCTCTGTTGG+CGG + chr6.4:46110331-46110350 MS.gene062658:CDS 50.0%
GGAAATCCGGATTATCGGGT+TGG + chr6.4:46110708-46110727 MS.gene062658:CDS 50.0%
GGAAGAAAATGACGACGGGA+AGG - chr6.4:46110396-46110415 None:intergenic 50.0%
GGCACATCACCTTGCAGTTA+GGG + chr6.4:46110666-46110685 MS.gene062658:CDS 50.0%
GTTAGAGCGACAACAAGCGT+CGG - chr6.4:46110188-46110207 None:intergenic 50.0%
TAATGTAAGCGGGCCGAATG+CGG + chr6.4:46110876-46110895 MS.gene062658:CDS 50.0%
TCCGGGAAATCCGGATTATC+GGG + chr6.4:46110704-46110723 MS.gene062658:CDS 50.0%
TGGCACATCACCTTGCAGTT+AGG + chr6.4:46110665-46110684 MS.gene062658:CDS 50.0%
TTCATTGCTGGAGACAGTGC+CGG + chr6.4:46110634-46110653 MS.gene062658:CDS 50.0%
TTGGTGGAAACGCCGTTAAC+GGG - chr6.4:46110271-46110290 None:intergenic 50.0%
TTTACCGGAGGGATTGAACC+GGG + chr6.4:46110840-46110859 MS.gene062658:CDS 50.0%
! AGAAGTGAAATGGTAGCGCG+GGG - chr6.4:46110154-46110173 None:intergenic 50.0%
! GACGGGAAGGGAGGTTATTT+TGG - chr6.4:46110383-46110402 None:intergenic 50.0%
!! AGACAGTGCCGGTGCAAATT+TGG + chr6.4:46110645-46110664 MS.gene062658:CDS 50.0%
!! CCCGTCGTCATTTTCTTCCA+CGG + chr6.4:46110397-46110416 MS.gene062658:CDS 50.0%
!! GACGCTTGTTGTCGCTCTAA+CGG + chr6.4:46110187-46110206 MS.gene062658:CDS 50.0%
!! GTCATTTTCTTCCACGGTGG+TGG + chr6.4:46110403-46110422 MS.gene062658:CDS 50.0%
!! GTCGCAATATGATGACGGAG+AGG + chr6.4:46110546-46110565 MS.gene062658:CDS 50.0%
!! GTCGTCATTTTCTTCCACGG+TGG + chr6.4:46110400-46110419 MS.gene062658:CDS 50.0%
!!! GTGGAAGGTGTTTTTACCGG+AGG + chr6.4:46110828-46110847 MS.gene062658:CDS 50.0%
!!! TCAGCCTTTTTTTGGTGGGG+AGG + chr6.4:46110738-46110757 MS.gene062658:CDS 50.0%
AACGGGAGTGGCATTAGGTG+AGG - chr6.4:46110254-46110273 None:intergenic 55.0%
AGGACTTCTCCGCCAACAGA+GGG - chr6.4:46110343-46110362 None:intergenic 55.0%
CCCGACAAATCCTCCGCATT+CGG - chr6.4:46110892-46110911 None:intergenic 55.0%
CCGAATGCGGAGGATTTGTC+GGG + chr6.4:46110889-46110908 MS.gene062658:CDS 55.0%
CCGTTAACGGGAGTGGCATT+AGG - chr6.4:46110259-46110278 None:intergenic 55.0%
CGGGGCTTCCAAGGAATAAG+GGG - chr6.4:46110136-46110155 None:intergenic 55.0%
GAAAATGACGACGGGAAGGG+AGG - chr6.4:46110392-46110411 None:intergenic 55.0%
GAGGGATTGAACCGGGATCA+TGG + chr6.4:46110847-46110866 MS.gene062658:CDS 55.0%
GATAGTGATGCTCCGGTGTG+AGG - chr6.4:46110525-46110544 None:intergenic 55.0%
GATAGTTGAAGCCACCACCG+TGG - chr6.4:46110417-46110436 None:intergenic 55.0%
GCCGAATGCGGAGGATTTGT+CGG + chr6.4:46110888-46110907 MS.gene062658:CDS 55.0%
GCGGGGCTTCCAAGGAATAA+GGG - chr6.4:46110137-46110156 None:intergenic 55.0%
GGAGAAGTCCTCGGATATGG+TGG + chr6.4:46110352-46110371 MS.gene062658:CDS 55.0%
GGGTTTGTAAAGCAGCGCTC+CGG + chr6.4:46110686-46110705 MS.gene062658:CDS 55.0%
GGTTTGTAAAGCAGCGCTCC+GGG + chr6.4:46110687-46110706 MS.gene062658:CDS 55.0%
GTCCTCACCATCGACACCAA+CGG - chr6.4:46110799-46110818 None:intergenic 55.0%
TCTGTTGGCGGAGAAGTCCT+CGG + chr6.4:46110343-46110362 MS.gene062658:CDS 55.0%
TGATCCCGGTTCAATCCCTC+CGG - chr6.4:46110847-46110866 None:intergenic 55.0%
TGCGACGGATAGTGATGCTC+CGG - chr6.4:46110532-46110551 None:intergenic 55.0%
TGCGGAGGATTTGTCGGGTT+TGG + chr6.4:46110894-46110913 MS.gene062658:CDS 55.0%
TGGTTGCACCACCATATCCG+AGG - chr6.4:46110363-46110382 None:intergenic 55.0%
TGTCAACTACCGCCTCACAC+CGG + chr6.4:46110510-46110529 MS.gene062658:CDS 55.0%
TGTCGATGGTGAGGACGGAT+TGG + chr6.4:46110803-46110822 MS.gene062658:CDS 55.0%
!! GTTGGTGTCGATGGTGAGGA+CGG + chr6.4:46110798-46110817 MS.gene062658:CDS 55.0%
AAGCAGCGCTCCGGGAAATC+CGG + chr6.4:46110695-46110714 MS.gene062658:CDS 60.0%
AGTGATGCTCCGGTGTGAGG+CGG - chr6.4:46110522-46110541 None:intergenic 60.0%
CGCGGGGCTTCCAAGGAATA+AGG - chr6.4:46110138-46110157 None:intergenic 60.0%
GAGGACTTCTCCGCCAACAG+AGG - chr6.4:46110344-46110363 None:intergenic 60.0%
GGAAACGCCGTTAACGGGAG+TGG - chr6.4:46110266-46110285 None:intergenic 60.0%
GGCGGAGAAGTCCTCGGATA+TGG + chr6.4:46110349-46110368 MS.gene062658:CDS 60.0%
!! CGATGGTGAGGACGGATTGG+TGG + chr6.4:46110806-46110825 MS.gene062658:CDS 60.0%
!! GAGGACGGATTGGTGGTGGA+AGG + chr6.4:46110813-46110832 MS.gene062658:CDS 60.0%
!! TGGATCGCCGTTGGTGTCGA+TGG + chr6.4:46110789-46110808 MS.gene062658:CDS 60.0%
!! TGGTGAGGACGGATTGGTGG+TGG + chr6.4:46110809-46110828 MS.gene062658:CDS 60.0%
TGTAAGCGGGCCGAATGCGG+AGG + chr6.4:46110879-46110898 MS.gene062658:CDS 65.0%
! ATGGTAGCGCGGGGCTTCCA+AGG - chr6.4:46110145-46110164 None:intergenic 65.0%
!! CGCCGTTGGTGTCGATGGTG+AGG + chr6.4:46110794-46110813 MS.gene062658:CDS 65.0%
!! TGGGGAGGAACGGACTGAGG+CGG + chr6.4:46110753-46110772 MS.gene062658:CDS 65.0%
!! TGGTGGGGAGGAACGGACTG+AGG + chr6.4:46110750-46110769 MS.gene062658:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 46110079 46111134 46110079 ID=MS.gene062658
chr6.4 mRNA 46110079 46111134 46110079 ID=MS.gene062658.t1;Parent=MS.gene062658
chr6.4 exon 46110079 46111134 46110079 ID=MS.gene062658.t1.exon1;Parent=MS.gene062658.t1
chr6.4 CDS 46110079 46111134 46110079 ID=cds.MS.gene062658.t1;Parent=MS.gene062658.t1
Gene Sequence

>MS.gene062658

ATGATAGATGAGGAGGACAATATGTCACACTCAAACTCAACAAACAAACCCAAACCCCTTATTCCTTGGAAGCCCCGCGCTACCATTTCACTTCTTTCATTTTTAACCGACGCTTGTTGTCGCTCTAACGGCACATTCAACCGTCGTCTCTTTAACTTCTTCATCCGCAAATCCTCACCTAATGCCACTCCCGTTAACGGCGTTTCCACCAAAGACATTACCGTTAACCCCGAAAATAATGTTTGGTTCCGTCTCTTTACACCCTCTGTTGGCGGAGAAGTCCTCGGATATGGTGGTGCAACCAAAATAACCTCCCTTCCCGTCGTCATTTTCTTCCACGGTGGTGGCTTCAACTATCTTTGCCCCTCCTCAATCTACTATGATGCTTTTTGCCGTAGACTCTGCCAGCAAATCTCCGCTGTTGTTGTCTCTGTCAACTACCGCCTCACACCGGAGCATCACTATCCGTCGCAATATGATGACGGAGAGGCTGTTTTGAAATATTTGGATGAGAATAAAACGGTTTTACCGGAAAATGCTGATGTGTCAAAATGTTTCATTGCTGGAGACAGTGCCGGTGCAAATTTGGCACATCACCTTGCAGTTAGGGTTTGTAAAGCAGCGCTCCGGGAAATCCGGATTATCGGGTTGGTATCGATTCAGCCTTTTTTTGGTGGGGAGGAACGGACTGAGGCGGAGATTAAACTTGTTGGATCGCCGTTGGTGTCGATGGTGAGGACGGATTGGTGGTGGAAGGTGTTTTTACCGGAGGGATTGAACCGGGATCATGGTGCTGTTAATGTAAGCGGGCCGAATGCGGAGGATTTGTCGGGTTTGGATTATCCGAATACGCTTGTTTTTATTGGTGGGTTTGATCCTTTAAATGATTGGCAAAAGAGGTATTATGATTGGTTGAAGAAATGTGGGAAAAAGGTTGAGTTGATTGAATATCCAACCATGATTCATGCTTTTTATATATTTCCTGACTTGCTTGAATCTGCTCAGTTGATTATGCAAGTCAAGGAGTTTGTCAACAAAGTCTCCGGTTCTAAATGA
Protein sequence

>MS.gene062658.t1

MIDEEDNMSHSNSTNKPKPLIPWKPRATISLLSFLTDACCRSNGTFNRRLFNFFIRKSSPNATPVNGVSTKDITVNPENNVWFRLFTPSVGGEVLGYGGATKITSLPVVIFFHGGGFNYLCPSSIYYDAFCRRLCQQISAVVVSVNYRLTPEHHYPSQYDDGEAVLKYLDENKTVLPENADVSKCFIAGDSAGANLAHHLAVRVCKAALREIRIIGLVSIQPFFGGEERTEAEIKLVGSPLVSMVRTDWWWKVFLPEGLNRDHGAVNVSGPNAEDLSGLDYPNTLVFIGGFDPLNDWQKRYYDWLKKCGKKVELIEYPTMIHAFYIFPDLLESAQLIMQVKEFVNKVSGSK