AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062670.t1 MTR_6g065110 89.320 309 27 1 5 307 2 310 0.0 570
MS.gene062670.t1 MTR_6g065120 74.138 290 74 1 5 293 2 291 2.69e-165 463
MS.gene062670.t1 MTR_6g065400 72.569 288 79 0 6 293 2 289 9.22e-157 441
MS.gene062670.t1 MTR_6g065370 71.528 288 82 0 6 293 2 289 3.87e-156 440
MS.gene062670.t1 MTR_6g065270 69.492 295 81 2 3 293 2 291 2.22e-153 433
MS.gene062670.t1 MTR_6g065340 67.918 293 78 1 1 293 8 284 4.10e-142 404
MS.gene062670.t1 MTR_6g065430 55.749 287 116 3 9 293 6 283 1.24e-112 329
MS.gene062670.t1 MTR_1g082210 40.645 310 175 3 2 307 4 308 5.75e-74 231
MS.gene062670.t1 MTR_7g093950 42.807 285 154 5 15 291 17 300 2.12e-73 229
MS.gene062670.t1 MTR_1g089310 42.553 282 153 3 15 291 17 294 1.51e-71 225
MS.gene062670.t1 MTR_1g089310 42.553 282 153 3 15 291 33 310 2.57e-71 224
MS.gene062670.t1 MTR_6g464280 36.842 266 154 5 36 294 24 282 1.54e-49 167
MS.gene062670.t1 MTR_6g464510 39.301 229 128 4 35 260 27 247 2.05e-48 164
MS.gene062670.t1 MTR_8g035500 34.831 267 154 7 36 292 17 273 3.01e-44 153
MS.gene062670.t1 MTR_8g035540 32.721 272 163 5 35 295 13 275 5.24e-44 153
MS.gene062670.t1 MTR_6g464320 34.962 266 159 5 36 294 25 283 3.19e-43 151
MS.gene062670.t1 MTR_1g102170 37.313 201 113 3 63 260 69 259 1.95e-42 149
MS.gene062670.t1 MTR_0007s0520 38.095 210 115 4 57 260 44 244 4.01e-42 148
MS.gene062670.t1 MTR_7g406880 34.884 258 143 7 51 289 46 297 2.34e-40 144
MS.gene062670.t1 MTR_1g012430 34.914 232 137 4 59 287 35 255 2.90e-40 143
MS.gene062670.t1 MTR_8g035520 34.538 249 142 6 57 294 44 282 7.24e-40 142
MS.gene062670.t1 MTR_6g464120 35.498 231 130 5 36 260 29 246 2.80e-39 144
MS.gene062670.t1 MTR_1g102160 32.472 271 157 6 9 272 4 255 1.21e-38 139
MS.gene062670.t1 MTR_1g111280 33.712 264 146 9 51 299 51 300 1.47e-34 129
MS.gene062670.t1 MTR_4g086510 34.728 239 139 5 57 289 39 266 3.04e-33 124
MS.gene062670.t1 MTR_4g050140 31.197 234 144 3 64 289 42 266 4.60e-32 121
MS.gene062670.t1 MTR_7g056207 34.328 201 117 3 61 255 75 266 4.91e-32 122
MS.gene062670.t1 MTR_4g086480 31.381 239 144 4 57 289 43 267 2.78e-31 119
MS.gene062670.t1 MTR_6g022710 32.234 273 127 7 84 307 137 400 3.59e-31 121
MS.gene062670.t1 MTR_4g086540 35.135 185 109 3 42 224 33 208 2.82e-30 116
MS.gene062670.t1 MTR_7g107040 29.644 253 154 5 48 289 28 267 5.86e-30 116
MS.gene062670.t1 MTR_4g086470 31.915 235 140 4 61 289 46 266 6.81e-30 115
MS.gene062670.t1 MTR_3g012440 33.831 201 127 1 95 289 84 284 9.21e-29 113
MS.gene062670.t1 MTR_3g101420 29.876 241 142 5 57 287 38 261 8.45e-27 107
MS.gene062670.t1 MTR_3g450680 30.279 251 139 7 57 289 48 280 3.19e-26 106
MS.gene062670.t1 MTR_8g040860 29.200 250 149 6 57 291 48 284 8.41e-25 102
MS.gene062670.t1 MTR_5g005900 28.413 271 162 9 35 289 21 275 6.09e-24 99.8
MS.gene062670.t1 MTR_1g105020 28.253 269 160 9 36 289 21 271 1.15e-21 93.6
MS.gene062670.t1 MTR_6g065470 34.104 173 80 5 9 163 6 162 3.91e-21 88.6
MS.gene062670.t1 MTR_5g005850 30.601 183 104 5 57 226 45 217 9.74e-21 90.9
MS.gene062670.t1 MTR_5g005920 27.372 274 169 9 28 287 18 275 6.95e-20 88.6
MS.gene062670.t1 MTR_0007s0480 41.758 91 47 2 175 260 1 90 8.09e-17 77.0
MS.gene062670.t1 MTR_1g104930 29.965 287 158 10 18 287 9 269 9.97e-17 79.7
MS.gene062670.t1 MTR_7g051020 30.380 237 141 6 63 287 48 272 9.30e-15 73.9
MS.gene062670.t1 MTR_6g065480 50.877 57 27 1 237 293 1 56 5.01e-14 66.2
MS.gene062670.t1 MTR_1g104933 29.362 235 139 6 63 287 46 263 9.80e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062670.t1 AT5G23530 51.351 296 125 5 1 292 1 281 3.53e-109 320
MS.gene062670.t1 AT5G27320 42.414 290 149 3 11 291 13 293 7.42e-74 231
MS.gene062670.t1 AT3G63010 40.989 283 157 3 15 291 17 295 5.98e-68 216
MS.gene062670.t1 AT3G05120 39.175 291 167 3 15 300 17 302 9.38e-66 210
MS.gene062670.t1 AT5G62180 34.831 267 162 7 36 295 21 282 9.23e-48 163
MS.gene062670.t1 AT2G45600 33.198 247 142 5 62 295 38 274 8.87e-44 152
MS.gene062670.t1 AT1G68620 33.761 234 141 4 60 289 57 280 4.66e-40 143
MS.gene062670.t1 AT2G45610 31.452 248 151 5 36 272 22 261 6.95e-39 139
MS.gene062670.t1 AT5G16080 34.615 234 129 7 68 289 68 289 2.02e-35 131
MS.gene062670.t1 AT1G47480 33.190 232 140 4 57 287 39 256 1.02e-33 125
MS.gene062670.t1 AT5G06570 35.563 284 144 8 35 300 22 284 2.46e-33 125
MS.gene062670.t1 AT5G06570 35.563 284 144 8 35 300 22 284 2.46e-33 125
MS.gene062670.t1 AT5G06570 35.563 284 144 8 35 300 37 299 4.58e-33 124
MS.gene062670.t1 AT5G06570 35.563 284 144 8 35 300 37 299 4.58e-33 124
MS.gene062670.t1 AT3G27320 29.787 329 145 9 59 307 56 378 5.01e-31 120
MS.gene062670.t1 AT5G14310 32.075 265 129 6 87 307 139 396 5.10e-31 121
MS.gene062670.t1 AT3G48690 31.557 244 146 6 55 287 34 267 2.05e-30 117
MS.gene062670.t1 AT1G19190 32.917 240 137 7 57 287 36 260 2.71e-30 117
MS.gene062670.t1 AT3G48700 31.474 251 141 6 55 287 34 271 4.22e-30 116
MS.gene062670.t1 AT3G27320 31.724 290 142 9 60 307 135 410 1.40e-29 117
MS.gene062670.t1 AT2G03550 33.613 238 133 6 56 285 35 255 7.78e-29 112
MS.gene062670.t1 AT1G49640 28.629 248 162 7 56 300 36 271 1.30e-25 104
MS.gene062670.t1 AT1G49660 31.298 262 159 9 33 285 13 262 6.25e-25 102
MS.gene062670.t1 AT1G49650 29.297 256 160 7 53 300 88 330 1.22e-23 99.8

Find 100 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATGCCGAAAATAAAATT+TGG 0.244137 6.4:+45817510 MS.gene062670:CDS
GGACAGCGCGGGTGCAAATT+TGG 0.251377 6.4:+45817853 MS.gene062670:CDS
GGCACATCATGTTGCGGTTC+GGG 0.272011 6.4:+45817874 MS.gene062670:CDS
CTTTAAAGGAAAAGGGAGTT+TGG 0.286360 6.4:-45817336 None:intergenic
ATCGAAAGGCGGGTCTTTAA+AGG 0.300157 6.4:-45817350 None:intergenic
AACCGATTACCCGAATTCTT+TGG 0.307430 6.4:-45817913 None:intergenic
GCGGTTCGGGCTTGCAAAGC+GGG 0.308999 6.4:+45817887 MS.gene062670:CDS
CACAGGAAGGGAGGTGGTTT+CGG 0.337864 6.4:-45817588 None:intergenic
AAGCGGGGCTCCAAAGAATT+CGG 0.351399 6.4:+45817903 MS.gene062670:CDS
TGGCACATCATGTTGCGGTT+CGG 0.360768 6.4:+45817873 MS.gene062670:CDS
TTCATTGATTGTGACTTTAA+CGG 0.364358 6.4:+45817373 MS.gene062670:CDS
AAATCATCAACGACCCTTCA+AGG 0.373985 6.4:-45817991 None:intergenic
CTCCAAAGAATTCGGGTAAT+CGG 0.378183 6.4:+45817911 MS.gene062670:CDS
TCTCCGACAAATGGTATCAT+AGG 0.400432 6.4:-45817663 None:intergenic
TTTGTGGAAACACCGTTAAT+GGG 0.405974 6.4:-45817476 None:intergenic
CAACGACGACATTCATTTCC+CGG 0.406758 6.4:-45817691 None:intergenic
GTTGAGAAGACGGCGGTTAA+CGG 0.412536 6.4:-45817420 None:intergenic
TTTAAAGGAAAAGGGAGTTT+GGG 0.419670 6.4:-45817335 None:intergenic
GTCAGAATAAGTGTTGCTTA+TGG 0.421076 6.4:+45818829 MS.gene062670:intron
CCGGATAAATCCTCCGCATT+CGG 0.425373 6.4:-45818097 None:intergenic
GTCAATGACGGCGCCGGAGG+TGG 0.430884 6.4:+45817557 MS.gene062670:CDS
CTCCATCTTCGTATTGTGTC+GGG 0.432185 6.4:-45817751 None:intergenic
AGCGGGGCTCCAAAGAATTC+GGG 0.432561 6.4:+45817904 MS.gene062670:CDS
TTTATCCGGATTAAATTATC+CGG 0.439009 6.4:+45818111 MS.gene062670:CDS
TTCAAGGCGAATCTCGGCTT+CGG 0.441952 6.4:-45817975 None:intergenic
AGGCGGGTCTTTAAAGGAAA+AGG 0.448184 6.4:-45817344 None:intergenic
GGTTGGATGGGTTATATGAT+TGG 0.450683 6.4:+45818155 MS.gene062670:CDS
CATTAGCCGCGCTATGATCT+CGG 0.455761 6.4:-45818066 None:intergenic
GCTAAAGCAACTCCCATTAA+CGG 0.459397 6.4:+45817464 MS.gene062670:CDS
TTTCGATGGCGAGGACGGAT+TGG 0.463866 6.4:+45818011 MS.gene062670:CDS
TCTCCATCTTCGTATTGTGT+CGG 0.466415 6.4:-45817752 None:intergenic
TGTCAAAATGTTTCCTAGCT+GGG 0.479793 6.4:+45817831 MS.gene062670:CDS
ATGATATTCTTCCACGGTGG+AGG 0.480650 6.4:+45817611 MS.gene062670:CDS
CCATTTGTCGGAGATTCTCC+CGG 0.483164 6.4:+45817672 MS.gene062670:CDS
CGGTGTGCGGCGGTAGTTGA+CGG 0.483989 6.4:-45817717 None:intergenic
CCGAATGCGGAGGATTTATC+CGG 0.484693 6.4:+45818097 MS.gene062670:CDS
CCGGGAGAATCTCCGACAAA+TGG 0.494532 6.4:-45817672 None:intergenic
CTCGTCGAGGAATTTCAATA+CGG 0.501120 6.4:-45817777 None:intergenic
TATCCTATGATACCATTTGT+CGG 0.501388 6.4:+45817660 MS.gene062670:CDS
TGGCGAGGACGGATTGGATG+TGG 0.502768 6.4:+45818017 MS.gene062670:CDS
GTGTCAAAATGTTTCCTAGC+TGG 0.506781 6.4:+45817830 MS.gene062670:CDS
GACGCCGCATGTCGATCTAA+TGG 0.508766 6.4:+45817395 MS.gene062670:CDS
GAAGCCGAGATTCGCCTTGA+AGG 0.512423 6.4:+45817977 MS.gene062670:CDS
GGCGCCGTCATTGACGCCGG+TGG 0.516332 6.4:-45817549 None:intergenic
TTCGGTGTCACTACCACCTC+CGG 0.517098 6.4:-45817570 None:intergenic
GATGATTTCGATGGCGAGGA+CGG 0.517371 6.4:+45818006 MS.gene062670:CDS
TGTATCCGGATAATTTAATC+CGG 0.527100 6.4:-45818116 None:intergenic
CATTATCATGTGAGATGAGA+AGG 0.527608 6.4:-45818852 None:intergenic
ACTCCCACCGGCGTCAATGA+CGG 0.528187 6.4:+45817545 MS.gene062670:CDS
CATTTGTCGGAGATTCTCCC+GGG 0.529843 6.4:+45817673 MS.gene062670:CDS
AATCAATGAAATCGAAAGGC+GGG 0.532050 6.4:-45817360 None:intergenic
GGTTATATGATTGGCAAAAG+AGG 0.534905 6.4:+45818164 MS.gene062670:CDS
TTCCTAGCTGGGGACAGCGC+GGG 0.543364 6.4:+45817842 MS.gene062670:CDS
CGACCCTTCAAGGCGAATCT+CGG 0.547607 6.4:-45817981 None:intergenic
AACGACGACATTCATTTCCC+GGG 0.549284 6.4:-45817690 None:intergenic
CGGTTCGGGCTTGCAAAGCG+GGG 0.549616 6.4:+45817888 MS.gene062670:CDS
CACCCGCGCTGTCCCCAGCT+AGG 0.551572 6.4:-45817844 None:intergenic
GGCGTCAATGACGGCGCCGG+AGG 0.551968 6.4:+45817554 MS.gene062670:CDS
ACCGGCGTCAATGACGGCGC+CGG 0.560434 6.4:+45817551 MS.gene062670:CDS
CATAGCGCGGCTAATGTAAG+TGG 0.562022 6.4:+45818073 MS.gene062670:CDS
TGATCTCGGTTCGAACCCTC+CGG 0.563155 6.4:-45818052 None:intergenic
AAGCCGAGATTCGCCTTGAA+GGG 0.564157 6.4:+45817978 MS.gene062670:CDS
TGCGGTTCGGGCTTGCAAAG+CGG 0.569535 6.4:+45817886 MS.gene062670:CDS
TTCCGTCTCTTCACTCCCAC+CGG 0.570097 6.4:+45817533 MS.gene062670:CDS
GCGCCGTCATTGACGCCGGT+GGG 0.575778 6.4:-45817548 None:intergenic
GTATCATAGGATAATGAAGC+GGG 0.580476 6.4:-45817650 None:intergenic
CGCCGGTGGGAGTGAAGAGA+CGG 0.581353 6.4:-45817535 None:intergenic
GGCGGGTCTTTAAAGGAAAA+GGG 0.583638 6.4:-45817343 None:intergenic
TCCGGCGCCGTCATTGACGC+CGG 0.588664 6.4:-45817552 None:intergenic
AAATTTGGCACATCATGTTG+CGG 0.593551 6.4:+45817868 MS.gene062670:CDS
TATCATCACAGGAAGGGAGG+TGG 0.596027 6.4:-45817594 None:intergenic
GTCAAAATGTTTCCTAGCTG+GGG 0.597118 6.4:+45817832 MS.gene062670:CDS
TCACAATCAATGAAATCGAA+AGG 0.599759 6.4:-45817364 None:intergenic
TGTAAGTGGGCCGAATGCGG+AGG 0.602303 6.4:+45818087 MS.gene062670:CDS
GGAAGAATATCATCACAGGA+AGG 0.605245 6.4:-45817601 None:intergenic
CCGTGGAAGAATATCATCAC+AGG 0.606907 6.4:-45817605 None:intergenic
CCTGTGATGATATTCTTCCA+CGG 0.607650 6.4:+45817605 MS.gene062670:CDS
TACCCGACACAATACGAAGA+TGG 0.608658 6.4:+45817749 MS.gene062670:CDS
TATCTAGAAAGTTGAGAAGA+CGG 0.610452 6.4:-45817430 None:intergenic
AGGGTCGTTGATGATTTCGA+TGG 0.612939 6.4:+45817997 MS.gene062670:CDS
AGCGATACTCCGGTGTGCGG+CGG 0.618464 6.4:-45817727 None:intergenic
CGTTGATGATTTCGATGGCG+AGG 0.625109 6.4:+45818002 MS.gene062670:CDS
TTTCCTAGCTGGGGACAGCG+CGG 0.627301 6.4:+45817841 MS.gene062670:CDS
GAAGAATATCATCACAGGAA+GGG 0.627988 6.4:-45817600 None:intergenic
CTTTCTAGATAACAAAACAT+CGG 0.628714 6.4:+45817442 MS.gene062670:CDS
TAATGTAAGTGGGCCGAATG+CGG 0.629761 6.4:+45818084 MS.gene062670:CDS
GAATATCATCACAGGAAGGG+AGG 0.633236 6.4:-45817597 None:intergenic
TGTGTCGGGTAGCGATACTC+CGG 0.641761 6.4:-45817737 None:intergenic
CGTCAACTACCGCCGCACAC+CGG 0.643888 6.4:+45817718 MS.gene062670:CDS
GGTAGCGATACTCCGGTGTG+CGG 0.653707 6.4:-45817730 None:intergenic
CAATCAATGAAATCGAAAGG+CGG 0.654273 6.4:-45817361 None:intergenic
AAACGGATTTGTTCTCGTCG+AGG 0.660434 6.4:-45817790 None:intergenic
GAGGACGGATTGGATGTGGA+AGG 0.674137 6.4:+45818021 MS.gene062670:CDS
GGTATCATAGGATAATGAAG+CGG 0.685454 6.4:-45817651 None:intergenic
ATAGCGCGGCTAATGTAAGT+GGG 0.688902 6.4:+45818074 MS.gene062670:CDS
CTAGAAAGTTGAGAAGACGG+CGG 0.738759 6.4:-45817427 None:intergenic
GTGATGATATTCTTCCACGG+TGG 0.743450 6.4:+45817608 MS.gene062670:CDS
TTCGAACCGAGATCATAGCG+CGG 0.747041 6.4:+45818060 MS.gene062670:CDS
TAAATGTGAATCCTCCACCG+TGG 0.765187 6.4:-45817622 None:intergenic
GGTACCATTAGATCGACATG+CGG 0.784594 6.4:-45817399 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATCAATAGTTATAAG+TGG + chr6.4:45818591-45818610 MS.gene062670:intron 15.0%
!! AGTAATAATAAATTCCCATT+TGG + chr6.4:45818381-45818400 MS.gene062670:intron 20.0%
!! GTAATAATAAATTCCCATTT+GGG + chr6.4:45818382-45818401 MS.gene062670:intron 20.0%
!! GTGATTGTTTAAATTTCATT+TGG + chr6.4:45818450-45818469 MS.gene062670:intron 20.0%
!! TGAGTTAATATTCAAAACTA+TGG - chr6.4:45818501-45818520 None:intergenic 20.0%
!! TTAATGCCGAAAATAAAATT+TGG + chr6.4:45817510-45817529 MS.gene062670:CDS 20.0%
!!! AATTTTATTTTCGGCATTAA+CGG - chr6.4:45817510-45817529 None:intergenic 20.0%
!!! CAATACTAGATTTTGAATTA+AGG + chr6.4:45818519-45818538 MS.gene062670:intron 20.0%
!!! GCATCATCATTATTTAAAAT+TGG - chr6.4:45818335-45818354 None:intergenic 20.0%
! AACATCATAAACATACCATA+TGG - chr6.4:45818549-45818568 None:intergenic 25.0%
! CTTTCTAGATAACAAAACAT+CGG + chr6.4:45817442-45817461 MS.gene062670:CDS 25.0%
! GAAATTTAAACAATCACTTG+AGG - chr6.4:45818447-45818466 None:intergenic 25.0%
! GTTGAAAATATCTGGAAAAA+AGG + chr6.4:45818198-45818217 MS.gene062670:intron 25.0%
! TATGAATGGTTGAAAATATC+TGG + chr6.4:45818190-45818209 MS.gene062670:intron 25.0%
! TTTATCCGGATTAAATTATC+CGG + chr6.4:45818111-45818130 MS.gene062670:CDS 25.0%
!! ATGTTGCTTAATAAAGAGAA+AGG + chr6.4:45818564-45818583 MS.gene062670:intron 25.0%
!! TTCATTGATTGTGACTTTAA+CGG + chr6.4:45817373-45817392 MS.gene062670:CDS 25.0%
!!! ACGGAACCAAATTTTATTTT+CGG - chr6.4:45817519-45817538 None:intergenic 25.0%
!!! ATTTCAATGCAACCATTTTT+TGG + chr6.4:45817938-45817957 MS.gene062670:CDS 25.0%
!!! TCTGTTGATTAATTTGCTTA+TGG + chr6.4:45818788-45818807 MS.gene062670:intron 25.0%
!!! TGATTAATTTGCTTATGGTT+TGG + chr6.4:45818793-45818812 MS.gene062670:intron 25.0%
ATCATAAACATACCATATGG+TGG - chr6.4:45818546-45818565 None:intergenic 30.0%
TAAAAACCATGCATCATGTT+TGG - chr6.4:45818241-45818260 None:intergenic 30.0%
TATCCTATGATACCATTTGT+CGG + chr6.4:45817660-45817679 MS.gene062670:CDS 30.0%
TATCTAGAAAGTTGAGAAGA+CGG - chr6.4:45817433-45817452 None:intergenic 30.0%
TCACAATCAATGAAATCGAA+AGG - chr6.4:45817367-45817386 None:intergenic 30.0%
TGTATCCGGATAATTTAATC+CGG - chr6.4:45818119-45818138 None:intergenic 30.0%
TTTAAAGGAAAAGGGAGTTT+GGG - chr6.4:45817338-45817357 None:intergenic 30.0%
! ATTAACGGTTATGTCTTTTG+TGG - chr6.4:45817495-45817514 None:intergenic 30.0%
AAAATCAACTGAGAAGACTC+GGG - chr6.4:45818280-45818299 None:intergenic 35.0%
AAATTTGGCACATCATGTTG+CGG + chr6.4:45817868-45817887 MS.gene062670:CDS 35.0%
AATCAATGAAATCGAAAGGC+GGG - chr6.4:45817363-45817382 None:intergenic 35.0%
ACTACATATCATGACCCAAA+TGG - chr6.4:45818399-45818418 None:intergenic 35.0%
CAAAATCAACTGAGAAGACT+CGG - chr6.4:45818281-45818300 None:intergenic 35.0%
CAATCAATGAAATCGAAAGG+CGG - chr6.4:45817364-45817383 None:intergenic 35.0%
CATTATCATGTGAGATGAGA+AGG - chr6.4:45818855-45818874 None:intergenic 35.0%
CTACATATCATGACCCAAAT+GGG - chr6.4:45818398-45818417 None:intergenic 35.0%
GAAGAATATCATCACAGGAA+GGG - chr6.4:45817603-45817622 None:intergenic 35.0%
GAATATCCAAACATGATGCA+TGG + chr6.4:45818232-45818251 MS.gene062670:intron 35.0%
GGTATCATAGGATAATGAAG+CGG - chr6.4:45817654-45817673 None:intergenic 35.0%
GGTCATGATATGTAGTTTAG+TGG + chr6.4:45818403-45818422 MS.gene062670:intron 35.0%
GGTTATATGATTGGCAAAAG+AGG + chr6.4:45818164-45818183 MS.gene062670:CDS 35.0%
GTATCATAGGATAATGAAGC+GGG - chr6.4:45817653-45817672 None:intergenic 35.0%
TGTCAAAATGTTTCCTAGCT+GGG + chr6.4:45817831-45817850 MS.gene062670:CDS 35.0%
TTGAATTAAGGACCACCATA+TGG + chr6.4:45818531-45818550 MS.gene062670:intron 35.0%
TTTGTGGAAACACCGTTAAT+GGG - chr6.4:45817479-45817498 None:intergenic 35.0%
! ATCAGCATTTTCCGGTAAAA+CGG - chr6.4:45817810-45817829 None:intergenic 35.0%
! CTTTAAAGGAAAAGGGAGTT+TGG - chr6.4:45817339-45817358 None:intergenic 35.0%
! TTTGACACATCAGCATTTTC+CGG - chr6.4:45817818-45817837 None:intergenic 35.0%
! TTTTGTGGAAACACCGTTAA+TGG - chr6.4:45817480-45817499 None:intergenic 35.0%
!! GGCAAAAGAGGTATTATGAA+TGG + chr6.4:45818176-45818195 MS.gene062670:intron 35.0%
!! GTCAGAATAAGTGTTGCTTA+TGG + chr6.4:45818829-45818848 MS.gene062670:intron 35.0%
!!! AAAGGGAGTTTGGGTTTTTT+AGG - chr6.4:45817329-45817348 None:intergenic 35.0%
!!! ATACACTTGTTTTTGTGGGT+GGG + chr6.4:45818134-45818153 MS.gene062670:CDS 35.0%
!!! TCAATGCAACCATTTTTTGG+TGG + chr6.4:45817941-45817960 MS.gene062670:CDS 35.0%
AAATCATCAACGACCCTTCA+AGG - chr6.4:45817994-45818013 None:intergenic 40.0%
AACCGATTACCCGAATTCTT+TGG - chr6.4:45817916-45817935 None:intergenic 40.0%
CCCACAAAAACAAGTGTATC+CGG - chr6.4:45818133-45818152 None:intergenic 40.0%
CCTGTGATGATATTCTTCCA+CGG + chr6.4:45817605-45817624 MS.gene062670:CDS 40.0%
CTCCAAAGAATTCGGGTAAT+CGG + chr6.4:45817911-45817930 MS.gene062670:CDS 40.0%
CTCGTCGAGGAATTTCAATA+CGG - chr6.4:45817780-45817799 None:intergenic 40.0%
GCTAAAGCAACTCCCATTAA+CGG + chr6.4:45817464-45817483 MS.gene062670:CDS 40.0%
GGAAGAATATCATCACAGGA+AGG - chr6.4:45817604-45817623 None:intergenic 40.0%
GTCAAAATGTTTCCTAGCTG+GGG + chr6.4:45817832-45817851 MS.gene062670:CDS 40.0%
GTGTCAAAATGTTTCCTAGC+TGG + chr6.4:45817830-45817849 MS.gene062670:CDS 40.0%
TCTCCATCTTCGTATTGTGT+CGG - chr6.4:45817755-45817774 None:intergenic 40.0%
TCTCCGACAAATGGTATCAT+AGG - chr6.4:45817666-45817685 None:intergenic 40.0%
! CGAGAACAAATCCGTTTTAC+CGG + chr6.4:45817796-45817815 MS.gene062670:CDS 40.0%
!! GGTTGGATGGGTTATATGAT+TGG + chr6.4:45818155-45818174 MS.gene062670:CDS 40.0%
!!! CCGGATACACTTGTTTTTGT+GGG + chr6.4:45818130-45818149 MS.gene062670:CDS 40.0%
!!! GATACACTTGTTTTTGTGGG+TGG + chr6.4:45818133-45818152 MS.gene062670:CDS 40.0%
!!! TCCGGATACACTTGTTTTTG+TGG + chr6.4:45818129-45818148 MS.gene062670:CDS 40.0%
AACGACGACATTCATTTCCC+GGG - chr6.4:45817693-45817712 None:intergenic 45.0%
ATAGCGCGGCTAATGTAAGT+GGG + chr6.4:45818074-45818093 MS.gene062670:CDS 45.0%
ATCGAAAGGCGGGTCTTTAA+AGG - chr6.4:45817353-45817372 None:intergenic 45.0%
ATGATATTCTTCCACGGTGG+AGG + chr6.4:45817611-45817630 MS.gene062670:CDS 45.0%
CAACGACGACATTCATTTCC+CGG - chr6.4:45817694-45817713 None:intergenic 45.0%
CCGTGGAAGAATATCATCAC+AGG - chr6.4:45817608-45817627 None:intergenic 45.0%
CTAGAAAGTTGAGAAGACGG+CGG - chr6.4:45817430-45817449 None:intergenic 45.0%
CTCCATCTTCGTATTGTGTC+GGG - chr6.4:45817754-45817773 None:intergenic 45.0%
GAATATCATCACAGGAAGGG+AGG - chr6.4:45817600-45817619 None:intergenic 45.0%
GGTACCATTAGATCGACATG+CGG - chr6.4:45817402-45817421 None:intergenic 45.0%
GTGATGATATTCTTCCACGG+TGG + chr6.4:45817608-45817627 MS.gene062670:CDS 45.0%
TAAATGTGAATCCTCCACCG+TGG - chr6.4:45817625-45817644 None:intergenic 45.0%
TAATGTAAGTGGGCCGAATG+CGG + chr6.4:45818084-45818103 MS.gene062670:CDS 45.0%
TACCCGACACAATACGAAGA+TGG + chr6.4:45817749-45817768 MS.gene062670:CDS 45.0%
TGAGAAGACTCGGGAACATT+AGG - chr6.4:45818271-45818290 None:intergenic 45.0%
! AAACGGATTTGTTCTCGTCG+AGG - chr6.4:45817793-45817812 None:intergenic 45.0%
! AGGCGGGTCTTTAAAGGAAA+AGG - chr6.4:45817347-45817366 None:intergenic 45.0%
! GGCGGGTCTTTAAAGGAAAA+GGG - chr6.4:45817346-45817365 None:intergenic 45.0%
!! AGGGTCGTTGATGATTTCGA+TGG + chr6.4:45817997-45818016 MS.gene062670:CDS 45.0%
!!! ACTTGTTTTTGTGGGTGGGT+TGG + chr6.4:45818138-45818157 MS.gene062670:CDS 45.0%
!!! GATGTGGAAGGTGTTTTTGC+CGG + chr6.4:45818033-45818052 MS.gene062670:CDS 45.0%
!!! TTTTTGTGGGTGGGTTGGAT+GGG + chr6.4:45818143-45818162 MS.gene062670:CDS 45.0%
AAGCCGAGATTCGCCTTGAA+GGG + chr6.4:45817978-45817997 MS.gene062670:CDS 50.0%
AAGCGGGGCTCCAAAGAATT+CGG + chr6.4:45817903-45817922 MS.gene062670:CDS 50.0%
CATAGCGCGGCTAATGTAAG+TGG + chr6.4:45818073-45818092 MS.gene062670:CDS 50.0%
CATTAGCCGCGCTATGATCT+CGG - chr6.4:45818069-45818088 None:intergenic 50.0%
CATTTGTCGGAGATTCTCCC+GGG + chr6.4:45817673-45817692 MS.gene062670:CDS 50.0%
CCATTTGTCGGAGATTCTCC+CGG + chr6.4:45817672-45817691 MS.gene062670:CDS 50.0%
CCGAATGCGGAGGATTTATC+CGG + chr6.4:45818097-45818116 MS.gene062670:CDS 50.0%
CCGGATAAATCCTCCGCATT+CGG - chr6.4:45818100-45818119 None:intergenic 50.0%
GATGATTTCGATGGCGAGGA+CGG + chr6.4:45818006-45818025 MS.gene062670:CDS 50.0%
GTTGAGAAGACGGCGGTTAA+CGG - chr6.4:45817423-45817442 None:intergenic 50.0%
TATCATCACAGGAAGGGAGG+TGG - chr6.4:45817597-45817616 None:intergenic 50.0%
TGGCACATCATGTTGCGGTT+CGG + chr6.4:45817873-45817892 MS.gene062670:CDS 50.0%
TTCAAGGCGAATCTCGGCTT+CGG - chr6.4:45817978-45817997 None:intergenic 50.0%
TTCGAACCGAGATCATAGCG+CGG + chr6.4:45818060-45818079 MS.gene062670:CDS 50.0%
!! CGTTGATGATTTCGATGGCG+AGG + chr6.4:45818002-45818021 MS.gene062670:CDS 50.0%
!!! GCAACCATTTTTTGGTGGCG+AGG + chr6.4:45817946-45817965 MS.gene062670:CDS 50.0%
!!! GTTTTTGTGGGTGGGTTGGA+TGG + chr6.4:45818142-45818161 MS.gene062670:CDS 50.0%
!!! TGGAAGGTGTTTTTGCCGGA+GGG + chr6.4:45818037-45818056 MS.gene062670:CDS 50.0%
AGCGGGGCTCCAAAGAATTC+GGG + chr6.4:45817904-45817923 MS.gene062670:CDS 55.0%
CACAGGAAGGGAGGTGGTTT+CGG - chr6.4:45817591-45817610 None:intergenic 55.0%
CCGGGAGAATCTCCGACAAA+TGG - chr6.4:45817675-45817694 None:intergenic 55.0%
CGACCCTTCAAGGCGAATCT+CGG - chr6.4:45817984-45818003 None:intergenic 55.0%
CGCTCCTCGCCACCAAAAAA+TGG - chr6.4:45817953-45817972 None:intergenic 55.0%
GAAGCCGAGATTCGCCTTGA+AGG + chr6.4:45817977-45817996 MS.gene062670:CDS 55.0%
GACGCCGCATGTCGATCTAA+TGG + chr6.4:45817395-45817414 MS.gene062670:CDS 55.0%
GGCACATCATGTTGCGGTTC+GGG + chr6.4:45817874-45817893 MS.gene062670:CDS 55.0%
TGTGTCGGGTAGCGATACTC+CGG - chr6.4:45817740-45817759 None:intergenic 55.0%
TTCCGTCTCTTCACTCCCAC+CGG + chr6.4:45817533-45817552 MS.gene062670:CDS 55.0%
TTTCGATGGCGAGGACGGAT+TGG + chr6.4:45818011-45818030 MS.gene062670:CDS 55.0%
! TGATCTCGGTTCGAACCCTC+CGG - chr6.4:45818055-45818074 None:intergenic 55.0%
!! GAGGACGGATTGGATGTGGA+AGG + chr6.4:45818021-45818040 MS.gene062670:CDS 55.0%
!! TTCGGTGTCACTACCACCTC+CGG - chr6.4:45817573-45817592 None:intergenic 55.0%
!!! GTGGAAGGTGTTTTTGCCGG+AGG + chr6.4:45818036-45818055 MS.gene062670:CDS 55.0%
ACTCCCACCGGCGTCAATGA+CGG + chr6.4:45817545-45817564 MS.gene062670:CDS 60.0%
GGACAGCGCGGGTGCAAATT+TGG + chr6.4:45817853-45817872 MS.gene062670:CDS 60.0%
GGTAGCGATACTCCGGTGTG+CGG - chr6.4:45817733-45817752 None:intergenic 60.0%
TGTAAGTGGGCCGAATGCGG+AGG + chr6.4:45818087-45818106 MS.gene062670:CDS 60.0%
TTTCCTAGCTGGGGACAGCG+CGG + chr6.4:45817841-45817860 MS.gene062670:CDS 60.0%
! TGCGGTTCGGGCTTGCAAAG+CGG + chr6.4:45817886-45817905 MS.gene062670:CDS 60.0%
!! TGGCGAGGACGGATTGGATG+TGG + chr6.4:45818017-45818036 MS.gene062670:CDS 60.0%
AGCGATACTCCGGTGTGCGG+CGG - chr6.4:45817730-45817749 None:intergenic 65.0%
CGCCGGTGGGAGTGAAGAGA+CGG - chr6.4:45817538-45817557 None:intergenic 65.0%
CGGTGTGCGGCGGTAGTTGA+CGG - chr6.4:45817720-45817739 None:intergenic 65.0%
CGTCAACTACCGCCGCACAC+CGG + chr6.4:45817718-45817737 MS.gene062670:CDS 65.0%
TTCCTAGCTGGGGACAGCGC+GGG + chr6.4:45817842-45817861 MS.gene062670:CDS 65.0%
! CGGTTCGGGCTTGCAAAGCG+GGG + chr6.4:45817888-45817907 MS.gene062670:CDS 65.0%
! GCGGTTCGGGCTTGCAAAGC+GGG + chr6.4:45817887-45817906 MS.gene062670:CDS 65.0%
ACCGGCGTCAATGACGGCGC+CGG + chr6.4:45817551-45817570 MS.gene062670:CDS 70.0%
GCGCCGTCATTGACGCCGGT+GGG - chr6.4:45817551-45817570 None:intergenic 70.0%
TCCGGCGCCGTCATTGACGC+CGG - chr6.4:45817555-45817574 None:intergenic 70.0%
! GTCAATGACGGCGCCGGAGG+TGG + chr6.4:45817557-45817576 MS.gene062670:CDS 70.0%
CACCCGCGCTGTCCCCAGCT+AGG - chr6.4:45817847-45817866 None:intergenic 75.0%
GGCGCCGTCATTGACGCCGG+TGG - chr6.4:45817552-45817571 None:intergenic 75.0%
! GGCGTCAATGACGGCGCCGG+AGG + chr6.4:45817554-45817573 MS.gene062670:CDS 75.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 45817308 45818879 45817308 ID=MS.gene062670
chr6.4 mRNA 45817308 45818879 45817308 ID=MS.gene062670.t1;Parent=MS.gene062670
chr6.4 exon 45817308 45818185 45817308 ID=MS.gene062670.t1.exon1;Parent=MS.gene062670.t1
chr6.4 CDS 45817308 45818185 45817308 ID=cds.MS.gene062670.t1;Parent=MS.gene062670.t1
chr6.4 exon 45818834 45818879 45818834 ID=MS.gene062670.t1.exon2;Parent=MS.gene062670.t1
chr6.4 CDS 45818834 45818879 45818834 ID=cds.MS.gene062670.t1;Parent=MS.gene062670.t1
Gene Sequence

>MS.gene062670

ATGTCATCAGACTCATCACCTAAAAAACCCAAACTCCCTTTTCCTTTAAAGACCCGCCTTTCGATTTCATTGATTGTGACTTTAACGGACGCCGCATGTCGATCTAATGGTACCGTTAACCGCCGTCTTCTCAACTTTCTAGATAACAAAACATCGGCTAAAGCAACTCCCATTAACGGTGTTTCCACAAAAGACATAACCGTTAATGCCGAAAATAAAATTTGGTTCCGTCTCTTCACTCCCACCGGCGTCAATGACGGCGCCGGAGGTGGTAGTGACACCGAAACCACCTCCCTTCCTGTGATGATATTCTTCCACGGTGGAGGATTCACATTTATGTCTCCCGCTTCATTATCCTATGATACCATTTGTCGGAGATTCTCCCGGGAAATGAATGTCGTCGTTGTCTCCGTCAACTACCGCCGCACACCGGAGTATCGCTACCCGACACAATACGAAGATGGAGAAGCCGTATTGAAATTCCTCGACGAGAACAAATCCGTTTTACCGGAAAATGCTGATGTGTCAAAATGTTTCCTAGCTGGGGACAGCGCGGGTGCAAATTTGGCACATCATGTTGCGGTTCGGGCTTGCAAAGCGGGGCTCCAAAGAATTCGGGTAATCGGTTTGATTTCAATGCAACCATTTTTTGGTGGCGAGGAGCGAACCGAAGCCGAGATTCGCCTTGAAGGGTCGTTGATGATTTCGATGGCGAGGACGGATTGGATGTGGAAGGTGTTTTTGCCGGAGGGTTCGAACCGAGATCATAGCGCGGCTAATGTAAGTGGGCCGAATGCGGAGGATTTATCCGGATTAAATTATCCGGATACACTTGTTTTTGTGGGTGGGTTGGATGGGTTATATGATTGGCAAAAGAGGTATTATGAATGGTTGAAAATATCTGGAAAAAAGGCTCAGTTGATTGAATATCCAAACATGATGCATGGTTTTTATGCGTTTCCTAATGTTCCCGAGTCTTCTCAGTTGATTTTGCAAGTCAAAGATTTTATCAACAATAGAGTCTCCAATTTTAAATAATGATGATGCTTATTGTAATGATAATAACATCAATTAGTAATAATAAATTCCCATTTGGGTCATGATATGTAGTTTAGTGGTATGTGTTTATTTTGTAACCTCAAGTGATTGTTTAAATTTCATTTGGAATAAATGTAAAATAAAAATTAGTGCCATAGTTTTGAATATTAACTCAATACTAGATTTTGAATTAAGGACCACCATATGGTATGTTTATGATGTTGCTTAATAAAGAGAAAGGTTATTTAAAATCAATAGTTATAAGTGGTGCATTTTTATACAATGTCAAATAAATTGTGAAGTTTTTTAGATGTTTAAATAACATTTCTTATTAATTTAAATACAATGTGAATGATCTTCATTGCTGTTATGACAGTGTTCACATTTTGAAAGTTTATACTTTGAATTTAGAATTTGAGTTTTCATAATTTGATATGAAAGATCTGTTGATTAATTTGCTTATGGTTTGGAATTGATATACATGTCAGAATAAGTGTTGCTTATGGCCTTCTCATCTCACATGATAATGTTTGA
Protein sequence

>MS.gene062670.t1

MSSDSSPKKPKLPFPLKTRLSISLIVTLTDAACRSNGTVNRRLLNFLDNKTSAKATPINGVSTKDITVNAENKIWFRLFTPTGVNDGAGGGSDTETTSLPVMIFFHGGGFTFMSPASLSYDTICRRFSREMNVVVVSVNYRRTPEYRYPTQYEDGEAVLKFLDENKSVLPENADVSKCFLAGDSAGANLAHHVAVRACKAGLQRIRVIGLISMQPFFGGEERTEAEIRLEGSLMISMARTDWMWKVFLPEGSNRDHSAANVSGPNAEDLSGLNYPDTLVFVGGLDGLYDWQKRISVAYGLLISHDNV