Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063069.t1 | XP_024635233.1 | 96.2 | 442 | 6 | 1 | 1 | 431 | 1 | 442 | 5.20E-244 | 853.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063069.t1 | Q9XGM8 | 69.8 | 441 | 122 | 1 | 1 | 430 | 1 | 441 | 8.9e-184 | 644.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063069.t1 | A0A1S2Y7Q7 | 88.0 | 442 | 42 | 1 | 1 | 431 | 1 | 442 | 9.6e-224 | 785.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene063069 | 0.807768 | 4.30E-50 | -1.69E-46 |
| MS.gene049958 | MS.gene063069 | 0.800284 | 1.55E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene063069 | 0.804319 | 2.29E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene063069 | 0.818425 | 1.97E-52 | -1.69E-46 |
| MS.gene052190 | MS.gene063069 | 0.805166 | 1.52E-49 | -1.69E-46 |
| MS.gene053221 | MS.gene063069 | 0.80854 | 2.95E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene063069 | -0.822356 | 2.47E-53 | -1.69E-46 |
| MS.gene054467 | MS.gene063069 | 0.820463 | 6.77E-53 | -1.69E-46 |
| MS.gene054468 | MS.gene063069 | 0.833151 | 6.29E-56 | -1.69E-46 |
| MS.gene054576 | MS.gene063069 | 0.821519 | 3.87E-53 | -1.69E-46 |
| MS.gene054581 | MS.gene063069 | 0.80312 | 4.06E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene063069 | 0.822061 | 2.90E-53 | -1.69E-46 |
| MS.gene055778 | MS.gene063069 | 0.802716 | 4.92E-49 | -1.69E-46 |
| MS.gene057012 | MS.gene063069 | 0.800389 | 1.47E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene063069 | 0.802117 | 6.53E-49 | -1.69E-46 |
| MS.gene058829 | MS.gene063069 | 0.800511 | 1.39E-48 | -1.69E-46 |
| MS.gene059584 | MS.gene063069 | 0.802321 | 5.93E-49 | -1.69E-46 |
| MS.gene060553 | MS.gene063069 | 0.811812 | 5.81E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063069.t1 | MTR_3g118520 | 97.544 | 285 | 7 | 0 | 1 | 285 | 1 | 285 | 0.0 | 578 |
| MS.gene063069.t1 | MTR_3g118525 | 98.438 | 128 | 2 | 0 | 304 | 431 | 15 | 142 | 2.39e-86 | 259 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063069.t1 | AT4G38240 | 70.930 | 430 | 125 | 0 | 1 | 430 | 1 | 430 | 0.0 | 667 |
| MS.gene063069.t1 | AT4G38240 | 69.841 | 441 | 122 | 1 | 1 | 430 | 1 | 441 | 0.0 | 665 |
| MS.gene063069.t1 | AT4G38240 | 69.841 | 441 | 122 | 1 | 1 | 430 | 1 | 441 | 0.0 | 665 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAACATTGCTCAACAATT+TGG | 0.214206 | 3.3:+96018316 | MS.gene063069:CDS |
| GGATATGCCTTCGGCCATTT+TGG | 0.228883 | 3.3:-96017273 | None:intergenic |
| TGAAGTAGCTTCAAAGATTC+TGG | 0.231515 | 3.3:-96018580 | None:intergenic |
| TATCGCTCGGACTTCTTTCC+TGG | 0.251249 | 3.3:+96017207 | MS.gene063069:CDS |
| TTTGGGAAATGGCGAAATTT+TGG | 0.251374 | 3.3:+96010617 | None:intergenic |
| TAAAGGAAATCTTGAAGAAA+TGG | 0.304704 | 3.3:-96011490 | None:intergenic |
| TATCCATTCTAGGTCCATTA+TGG | 0.306360 | 3.3:+96015962 | MS.gene063069:intron |
| TCTTCTCTTTGTTGCAGCCT+TGG | 0.315376 | 3.3:+96010654 | MS.gene063069:CDS |
| CTTGAACAAGAGACTTGATT+TGG | 0.319300 | 3.3:-96011056 | None:intergenic |
| TGGACTTCATTCAGCTTGAT+TGG | 0.364035 | 3.3:-96017958 | None:intergenic |
| TTATATGTTCTGCATACTTC+GGG | 0.365657 | 3.3:-96017596 | None:intergenic |
| GGGATGAACTATCACCAAAA+TGG | 0.369625 | 3.3:+96017259 | MS.gene063069:CDS |
| CCTGGCCTTTCAGTTTGAAC+CGG | 0.375663 | 3.3:-96015499 | None:intergenic |
| GTTTCGTCATGGAATGATAA+TGG | 0.381929 | 3.3:+96015987 | MS.gene063069:CDS |
| AATGGACTTGAGCTATTTGT+TGG | 0.383291 | 3.3:+96018080 | MS.gene063069:CDS |
| CCATGACGAAACAGCCATAA+TGG | 0.392943 | 3.3:-96015976 | None:intergenic |
| AATACCTGTGATACAAATAA+AGG | 0.404682 | 3.3:-96011507 | None:intergenic |
| CTTTGATTACTTTGAAGCTA+TGG | 0.412034 | 3.3:+96015822 | MS.gene063069:CDS |
| TCAAAGTAATCAAAGAAATC+AGG | 0.439796 | 3.3:-96015814 | None:intergenic |
| AAAGGAAATCTTGAAGAAAT+GGG | 0.442893 | 3.3:-96011489 | None:intergenic |
| ACGAAGCGTATATTCCTTGT+TGG | 0.443306 | 3.3:+96018556 | MS.gene063069:CDS |
| GTGCCTAGGACAGCTTATAA+AGG | 0.447968 | 3.3:+96018508 | MS.gene063069:CDS |
| GTTATATGTTCTGCATACTT+CGG | 0.455588 | 3.3:-96017597 | None:intergenic |
| ATTTCCTTTATTTGTATCAC+AGG | 0.466127 | 3.3:+96011503 | MS.gene063069:CDS |
| GGTCATTACAAGTGGGCTCT+TGG | 0.466698 | 3.3:+96015633 | MS.gene063069:intron |
| GAAGTAGCTTCAAAGATTCT+GGG | 0.477829 | 3.3:-96018579 | None:intergenic |
| TCGGACTTCTTTCCTGGACT+AGG | 0.478118 | 3.3:+96017213 | MS.gene063069:CDS |
| GCGAAGTCTGCTCCAGAGAC+AGG | 0.486412 | 3.3:-96018232 | None:intergenic |
| AAATTCAGACGTCAAAAGTA+AGG | 0.488269 | 3.3:+96011673 | MS.gene063069:CDS |
| AGTGGCAGCTGTTGTCATCA+TGG | 0.497854 | 3.3:+96011325 | MS.gene063069:CDS |
| TAGTAAGCAATTAACTCTCC+TGG | 0.506538 | 3.3:-96015517 | None:intergenic |
| TGCAGACGAACTTTATCGCT+CGG | 0.520518 | 3.3:+96017194 | MS.gene063069:intron |
| AACAAGGAATATACGCTTCG+TGG | 0.520785 | 3.3:-96018554 | None:intergenic |
| CTTGGTGTTCATCTACATCC+AGG | 0.522745 | 3.3:+96010672 | MS.gene063069:CDS |
| GGCTACATTCTTGCTCTCGA+CGG | 0.523455 | 3.3:-96011035 | None:intergenic |
| GTATGCAGAACATATAACTT+TGG | 0.523714 | 3.3:+96017602 | MS.gene063069:CDS |
| TGGTATATTTCAAGAGTGGA+AGG | 0.526230 | 3.3:+96018336 | MS.gene063069:CDS |
| GCCAGCATCCATCCTAGTCC+AGG | 0.526290 | 3.3:-96017225 | None:intergenic |
| CAGAATTGATAGTCCTCTCC+AGG | 0.527635 | 3.3:-96011367 | None:intergenic |
| ACTTCTTTCCTGGACTAGGA+TGG | 0.531658 | 3.3:+96017217 | MS.gene063069:CDS |
| TCCTGGACTAGGATGGATGC+TGG | 0.534970 | 3.3:+96017224 | MS.gene063069:CDS |
| CCATTATGGCTGTTTCGTCA+TGG | 0.538200 | 3.3:+96015976 | MS.gene063069:CDS |
| GAAGGTCGACTGGAAGTCAA+TGG | 0.538850 | 3.3:+96018062 | MS.gene063069:intron |
| AATCAAGCTGAATGAAGTCC+AGG | 0.541494 | 3.3:+96017960 | MS.gene063069:CDS |
| TTCTCGACTTACAACAAGAA+AGG | 0.541791 | 3.3:+96011010 | MS.gene063069:CDS |
| ACTCCTTTATAAGCTGTCCT+AGG | 0.545192 | 3.3:-96018511 | None:intergenic |
| ATGTAATCGTGCTGATTACC+TGG | 0.547620 | 3.3:+96011349 | MS.gene063069:CDS |
| TTGAGCCGGTTCAAACTGAA+AGG | 0.550180 | 3.3:+96015494 | MS.gene063069:CDS |
| GGATGCTGGCTAGATCTACA+TGG | 0.553110 | 3.3:+96017238 | MS.gene063069:CDS |
| CCGGTTCAAACTGAAAGGCC+AGG | 0.554999 | 3.3:+96015499 | MS.gene063069:CDS |
| GGACTTGAGCTATTTGTTGG+AGG | 0.555050 | 3.3:+96018083 | MS.gene063069:CDS |
| TGGAGCAGACTTCGCTCAGA+AGG | 0.556130 | 3.3:+96018240 | MS.gene063069:CDS |
| CGATGAGCTATCTCATATGC+AGG | 0.562275 | 3.3:+96011706 | MS.gene063069:CDS |
| ATGATGACAACAGCTGCCAC+TGG | 0.562931 | 3.3:-96011323 | None:intergenic |
| TAATCTGATCAATGAGTGAT+CGG | 0.568391 | 3.3:-96010969 | None:intergenic |
| TGGATTGAATGAAGGTCGAC+TGG | 0.576838 | 3.3:+96018052 | MS.gene063069:intron |
| ACTTCCAGGATGGTGTGCCT+AGG | 0.576852 | 3.3:+96018494 | MS.gene063069:intron |
| AAGGCTGATTGATAAAGTCC+AGG | 0.584981 | 3.3:+96011204 | MS.gene063069:CDS |
| TTATCCAACCTCAGATGACA+TGG | 0.598293 | 3.3:+96015783 | MS.gene063069:intron |
| AATTTGGTATATTTCAAGAG+TGG | 0.599428 | 3.3:+96018332 | MS.gene063069:CDS |
| TATAAAGTATAAAGATCAGT+GGG | 0.600564 | 3.3:+96018288 | MS.gene063069:CDS |
| ATGTTCTGCATACTTCGGGT+CGG | 0.615835 | 3.3:-96017592 | None:intergenic |
| ACACATACTTATCCTTGTCA+AGG | 0.618408 | 3.3:-96015852 | None:intergenic |
| AACAGAGTCATCATTCTCGA+AGG | 0.620252 | 3.3:+96015681 | MS.gene063069:CDS |
| TTTCAGGAAAAGATGTACAA+AGG | 0.627774 | 3.3:+96011185 | MS.gene063069:intron |
| CAGAAGGCACGCAATATAGA+CGG | 0.629864 | 3.3:+96018256 | MS.gene063069:CDS |
| TCAAAGATTCTGGGCCAACA+AGG | 0.632429 | 3.3:-96018570 | None:intergenic |
| ATCGTGCTGATTACCTGGAG+AGG | 0.643089 | 3.3:+96011354 | MS.gene063069:CDS |
| GATCAGATTAGCTTGCAACA+AGG | 0.650241 | 3.3:+96010982 | MS.gene063069:CDS |
| ACTATCACCAAAATGGCCGA+AGG | 0.673152 | 3.3:+96017266 | MS.gene063069:CDS |
| GTATAAAGTATAAAGATCAG+TGG | 0.683838 | 3.3:+96018287 | MS.gene063069:CDS |
| GATGCTGGCTAGATCTACAT+GGG | 0.685395 | 3.3:+96017239 | MS.gene063069:CDS |
| TATGGCAACTCTCCTTGACA+AGG | 0.700285 | 3.3:+96015840 | MS.gene063069:CDS |
| AGATTAAAAGAGAATCACAA+AGG | 0.714494 | 3.3:+96017557 | MS.gene063069:intron |
| AATTACATACAGCAACAGCG+AGG | 0.779431 | 3.3:-96010824 | None:intergenic |
| CTGGATGTAGATGAACACCA+AGG | 0.780942 | 3.3:-96010671 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 96010625 | 96018612 | 96010625 | ID=MS.gene063069 |
| chr3.3 | mRNA | 96010625 | 96018612 | 96010625 | ID=MS.gene063069.t1;Parent=MS.gene063069 |
| chr3.3 | exon | 96010625 | 96010693 | 96010625 | ID=MS.gene063069.t1.exon1;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96010625 | 96010693 | 96010625 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96010786 | 96010836 | 96010786 | ID=MS.gene063069.t1.exon2;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96010786 | 96010836 | 96010786 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96010937 | 96011087 | 96010937 | ID=MS.gene063069.t1.exon3;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96010937 | 96011087 | 96010937 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96011191 | 96011225 | 96011191 | ID=MS.gene063069.t1.exon4;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96011191 | 96011225 | 96011191 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96011320 | 96011396 | 96011320 | ID=MS.gene063069.t1.exon5;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96011320 | 96011396 | 96011320 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96011479 | 96011524 | 96011479 | ID=MS.gene063069.t1.exon6;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96011479 | 96011524 | 96011479 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96011665 | 96011727 | 96011665 | ID=MS.gene063069.t1.exon7;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96011665 | 96011727 | 96011665 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96015484 | 96015550 | 96015484 | ID=MS.gene063069.t1.exon8;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96015484 | 96015550 | 96015484 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96015634 | 96015702 | 96015634 | ID=MS.gene063069.t1.exon9;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96015634 | 96015702 | 96015634 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96015797 | 96015866 | 96015797 | ID=MS.gene063069.t1.exon10;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96015797 | 96015866 | 96015797 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96015974 | 96016035 | 96015974 | ID=MS.gene063069.t1.exon11;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96015974 | 96016035 | 96015974 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96017199 | 96017287 | 96017199 | ID=MS.gene063069.t1.exon12;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96017199 | 96017287 | 96017199 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96017569 | 96017631 | 96017569 | ID=MS.gene063069.t1.exon13;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96017569 | 96017631 | 96017569 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96017919 | 96017981 | 96017919 | ID=MS.gene063069.t1.exon14;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96017919 | 96017981 | 96017919 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96018066 | 96018104 | 96018066 | ID=MS.gene063069.t1.exon15;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96018066 | 96018104 | 96018066 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96018187 | 96018357 | 96018187 | ID=MS.gene063069.t1.exon16;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96018187 | 96018357 | 96018187 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| chr3.3 | exon | 96018502 | 96018612 | 96018502 | ID=MS.gene063069.t1.exon17;Parent=MS.gene063069.t1 |
| chr3.3 | CDS | 96018502 | 96018612 | 96018502 | ID=cds.MS.gene063069.t1;Parent=MS.gene063069.t1 |
| Gene Sequence |
| Protein sequence |
