Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06672.t1 | XP_003602283.2 | 75 | 292 | 4 | 4 | 1 | 225 | 17 | 306 | 3.70E-84 | 321.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06672.t1 | Q43385 | 29.8 | 252 | 113 | 14 | 17 | 225 | 66 | 296 | 3.5e-06 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06672.t1 | G7J7H3 | 75.0 | 292 | 4 | 4 | 1 | 225 | 17 | 306 | 2.7e-84 | 321.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene06672 | 0.835453 | 1.66E-56 | -1.69E-46 |
| MS.gene049254 | MS.gene06672 | 0.817168 | 3.79E-52 | -1.69E-46 |
| MS.gene049350 | MS.gene06672 | 0.806265 | 8.95E-50 | -1.69E-46 |
| MS.gene049672 | MS.gene06672 | 0.801515 | 8.68E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene06672 | 0.83225 | 1.05E-55 | -1.69E-46 |
| MS.gene051719 | MS.gene06672 | 0.808388 | 3.17E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene06672 | 0.810754 | 9.85E-51 | -1.69E-46 |
| MS.gene052000 | MS.gene06672 | 0.803279 | 3.76E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene06672 | 0.829862 | 4.06E-55 | -1.69E-46 |
| MS.gene052773 | MS.gene06672 | 0.824292 | 8.73E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene06672 | 0.81554 | 8.77E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene06672 | 0.822948 | 1.80E-53 | -1.69E-46 |
| MS.gene053900 | MS.gene06672 | 0.803654 | 3.14E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene06672 | 0.831751 | 1.40E-55 | -1.69E-46 |
| MS.gene055818 | MS.gene06672 | 0.812164 | 4.87E-51 | -1.69E-46 |
| MS.gene056050 | MS.gene06672 | 0.817853 | 2.66E-52 | -1.69E-46 |
| MS.gene056595 | MS.gene06672 | 0.80694 | 6.45E-50 | -1.69E-46 |
| MS.gene057132 | MS.gene06672 | 0.863351 | 2.65E-64 | -1.69E-46 |
| MS.gene058625 | MS.gene06672 | 0.805323 | 1.41E-49 | -1.69E-46 |
| MS.gene05894 | MS.gene06672 | 0.816212 | 6.21E-52 | -1.69E-46 |
| MS.gene059625 | MS.gene06672 | 0.805651 | 1.20E-49 | -1.69E-46 |
| MS.gene060962 | MS.gene06672 | 0.801369 | 9.30E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene06672 | 0.801663 | 8.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06672.t1 | MTR_3g091820 | 73.630 | 292 | 8 | 5 | 1 | 225 | 17 | 306 | 6.42e-132 | 374 |
| MS.gene06672.t1 | MTR_8g027295 | 36.713 | 286 | 103 | 15 | 1 | 225 | 1 | 269 | 1.56e-32 | 119 |
| MS.gene06672.t1 | MTR_1g077600 | 32.946 | 258 | 91 | 13 | 14 | 216 | 30 | 260 | 3.27e-19 | 84.0 |
| MS.gene06672.t1 | MTR_1g077600 | 32.946 | 258 | 91 | 13 | 14 | 216 | 30 | 260 | 4.44e-19 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06672.t1 | AT3G61850 | 29.643 | 280 | 113 | 13 | 13 | 225 | 50 | 312 | 1.12e-22 | 94.0 |
| MS.gene06672.t1 | AT3G61850 | 29.643 | 280 | 113 | 13 | 13 | 225 | 62 | 324 | 2.03e-22 | 93.6 |
| MS.gene06672.t1 | AT3G61850 | 31.418 | 261 | 105 | 14 | 13 | 225 | 50 | 284 | 5.32e-21 | 89.0 |
| MS.gene06672.t1 | AT3G61850 | 31.418 | 261 | 105 | 14 | 13 | 225 | 50 | 284 | 5.32e-21 | 89.0 |
| MS.gene06672.t1 | AT3G61850 | 31.418 | 261 | 105 | 14 | 13 | 225 | 62 | 296 | 8.26e-21 | 88.6 |
Find 48 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGTCCTTGATGGATCTT+AGG | 0.169048 | 3.3:-76661947 | None:intergenic |
| TTTAAGCTTGGGGCTAATTT+TGG | 0.206492 | 3.3:+76662187 | MS.gene06672:CDS |
| TGTTCAAACTCACCACTATT+TGG | 0.210892 | 3.3:-76662361 | None:intergenic |
| AAGGTGATTCTACTGGTTAT+TGG | 0.303787 | 3.3:+76662401 | MS.gene06672:CDS |
| GAAGGGTTTGAGAATGGTTA+TGG | 0.323309 | 3.3:+76662214 | MS.gene06672:CDS |
| GGTTAAGATCTTGTCCTTGA+TGG | 0.347085 | 3.3:-76661955 | None:intergenic |
| GCAAAACTTGTAGAAGGTAT+TGG | 0.349440 | 3.3:+76661738 | MS.gene06672:intron |
| ATTAATAATCAAGAGGGTTC+TGG | 0.381524 | 3.3:+76662250 | MS.gene06672:CDS |
| CTTGTCCTTGATGGATCTTA+GGG | 0.390118 | 3.3:-76661946 | None:intergenic |
| GAGGGTTCTGGTGGTGGTGG+TGG | 0.395369 | 3.3:+76662262 | MS.gene06672:CDS |
| CAAGAGGGTTCTGGTGGTGG+TGG | 0.424985 | 3.3:+76662259 | MS.gene06672:CDS |
| TGAAAACTGCTTCTTCATCA+AGG | 0.432756 | 3.3:+76662086 | MS.gene06672:CDS |
| AAGCTCAATGAATTTAGATA+TGG | 0.438747 | 3.3:-76662017 | None:intergenic |
| AACTGATGATCATCTACAAC+AGG | 0.454576 | 3.3:-76662313 | None:intergenic |
| TTGTCTTGATCAGTTGTGTA+AGG | 0.463233 | 3.3:-76662040 | None:intergenic |
| GGTTTAGAAGGGTTTGAGAA+TGG | 0.472180 | 3.3:+76662208 | MS.gene06672:CDS |
| CTCTCTAACATAGAAGGTGT+TGG | 0.476502 | 3.3:-76661605 | None:intergenic |
| GATTCTACTGGTTATTGGAA+TGG | 0.482077 | 3.3:+76662406 | MS.gene06672:CDS |
| CTAGACTCAGACAAAGTCAT+AGG | 0.485051 | 3.3:-76662127 | None:intergenic |
| GAAAACTGCTTCTTCATCAA+GGG | 0.488666 | 3.3:+76662087 | MS.gene06672:CDS |
| CAGCAACAAATTCCAAATAG+TGG | 0.488831 | 3.3:+76662349 | MS.gene06672:CDS |
| TAATTATCCTCAGTTGTTTG+TGG | 0.490761 | 3.3:-76661989 | None:intergenic |
| AATCAAGAGGGTTCTGGTGG+TGG | 0.495161 | 3.3:+76662256 | MS.gene06672:CDS |
| TTGTTTGTGGTGGATAAGCT+AGG | 0.500159 | 3.3:-76661976 | None:intergenic |
| AATGGTTATGGTGGCTTGCA+AGG | 0.516585 | 3.3:+76662226 | MS.gene06672:CDS |
| TCTTTAAGAAATGTTCCTGT+AGG | 0.524165 | 3.3:+76661773 | MS.gene06672:CDS |
| GAATGTTCGGTGGAGGATCA+TGG | 0.528349 | 3.3:+76662428 | MS.gene06672:CDS |
| TTATCCTCAGTTGTTTGTGG+TGG | 0.529976 | 3.3:-76661986 | None:intergenic |
| AGACAAAACTACAAGTGCTA+TGG | 0.533697 | 3.3:+76662057 | MS.gene06672:CDS |
| AGAACTCAAGGTGATTCTAC+TGG | 0.537114 | 3.3:+76662394 | MS.gene06672:CDS |
| CATCAGCAAAATAGAACTCA+AGG | 0.544205 | 3.3:+76662382 | MS.gene06672:CDS |
| GGTTATTGGAATGGAATGTT+CGG | 0.551153 | 3.3:+76662415 | MS.gene06672:CDS |
| GGATTGGTTAAACAAATGGA+TGG | 0.552908 | 3.3:+76661563 | None:intergenic |
| TGGAATGGAATGTTCGGTGG+AGG | 0.558324 | 3.3:+76662421 | MS.gene06672:CDS |
| ATCAAGCTTTAAACTGTCCA+AGG | 0.561852 | 3.3:+76661648 | MS.gene06672:CDS |
| GGGTTTGAGAATGGTTATGG+TGG | 0.564934 | 3.3:+76662217 | MS.gene06672:CDS |
| AAAACTGCTTCTTCATCAAG+GGG | 0.565168 | 3.3:+76662088 | MS.gene06672:CDS |
| AGTGCAATGTATAATTCAAG+TGG | 0.566445 | 3.3:+76662148 | MS.gene06672:CDS |
| CAAAACCCTAAGATCCATCA+AGG | 0.571674 | 3.3:+76661941 | MS.gene06672:CDS |
| TATTGGAATGGAATGTTCGG+TGG | 0.576421 | 3.3:+76662418 | MS.gene06672:CDS |
| CAAGGGATTAATAATCAAGA+GGG | 0.577580 | 3.3:+76662244 | MS.gene06672:CDS |
| GCAAGGGATTAATAATCAAG+AGG | 0.579146 | 3.3:+76662243 | MS.gene06672:CDS |
| ATGGTTATGGTGGCTTGCAA+GGG | 0.581200 | 3.3:+76662227 | MS.gene06672:CDS |
| TGTAGAAGGTATTGGACTGA+AGG | 0.583395 | 3.3:+76661746 | MS.gene06672:intron |
| AGAAGGTATTGGACTGAAGG+TGG | 0.592159 | 3.3:+76661749 | MS.gene06672:intron |
| AATAATCAAGAGGGTTCTGG+TGG | 0.645196 | 3.3:+76662253 | MS.gene06672:CDS |
| TTATCCACCACAAACAACTG+AGG | 0.701982 | 3.3:+76661982 | MS.gene06672:CDS |
| CTATGTTAGAGAGAAAAGCA+AGG | 0.712069 | 3.3:+76661615 | MS.gene06672:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTTTGTTATTGATGA+TGG | - | chr3.3:76661866-76661885 | None:intergenic | 15.0% |
| ! | AAGCTCAATGAATTTAGATA+TGG | - | chr3.3:76662020-76662039 | None:intergenic | 25.0% |
| !!! | TTTAAAGCTTGATCTTTTTG+TGG | - | chr3.3:76661641-76661660 | None:intergenic | 25.0% |
| !!! | TTTTTTGTTATTGATGATGG+AGG | - | chr3.3:76661863-76661882 | None:intergenic | 25.0% |
| AGTGCAATGTATAATTCAAG+TGG | + | chr3.3:76662148-76662167 | MS.gene06672:CDS | 30.0% | |
| ATTAATAATCAAGAGGGTTC+TGG | + | chr3.3:76662250-76662269 | MS.gene06672:CDS | 30.0% | |
| CAAGGGATTAATAATCAAGA+GGG | + | chr3.3:76662244-76662263 | MS.gene06672:CDS | 30.0% | |
| TAATTATCCTCAGTTGTTTG+TGG | - | chr3.3:76661992-76662011 | None:intergenic | 30.0% | |
| ! | ACTTTTGCAAAACTTGTAGA+AGG | + | chr3.3:76661732-76661751 | MS.gene06672:intron | 30.0% |
| ! | CTTTGCAAGATTTTAAGCTT+GGG | + | chr3.3:76662176-76662195 | MS.gene06672:CDS | 30.0% |
| ! | TCTTTAAGAAATGTTCCTGT+AGG | + | chr3.3:76661773-76661792 | MS.gene06672:CDS | 30.0% |
| ! | TTTGCAAGATTTTAAGCTTG+GGG | + | chr3.3:76662177-76662196 | MS.gene06672:CDS | 30.0% |
| AAAACTGCTTCTTCATCAAG+GGG | + | chr3.3:76662088-76662107 | MS.gene06672:CDS | 35.0% | |
| AACTGATGATCATCTACAAC+AGG | - | chr3.3:76662316-76662335 | None:intergenic | 35.0% | |
| AGACAAAACTACAAGTGCTA+TGG | + | chr3.3:76662057-76662076 | MS.gene06672:CDS | 35.0% | |
| CAGCAACAAATTCCAAATAG+TGG | + | chr3.3:76662349-76662368 | MS.gene06672:CDS | 35.0% | |
| CATCAGCAAAATAGAACTCA+AGG | + | chr3.3:76662382-76662401 | MS.gene06672:CDS | 35.0% | |
| CTATGTTAGAGAGAAAAGCA+AGG | + | chr3.3:76661615-76661634 | MS.gene06672:CDS | 35.0% | |
| GAAAACTGCTTCTTCATCAA+GGG | + | chr3.3:76662087-76662106 | MS.gene06672:CDS | 35.0% | |
| GCAAGGGATTAATAATCAAG+AGG | + | chr3.3:76662243-76662262 | MS.gene06672:CDS | 35.0% | |
| GGTTATTGGAATGGAATGTT+CGG | + | chr3.3:76662415-76662434 | MS.gene06672:CDS | 35.0% | |
| TGAAAACTGCTTCTTCATCA+AGG | + | chr3.3:76662086-76662105 | MS.gene06672:CDS | 35.0% | |
| TGTTCAAACTCACCACTATT+TGG | - | chr3.3:76662364-76662383 | None:intergenic | 35.0% | |
| TTAAGAAATGTTCCTGTAGG+TGG | + | chr3.3:76661776-76661795 | MS.gene06672:intron | 35.0% | |
| TTGTCTTGATCAGTTGTGTA+AGG | - | chr3.3:76662043-76662062 | None:intergenic | 35.0% | |
| ! | AAGGTGATTCTACTGGTTAT+TGG | + | chr3.3:76662401-76662420 | MS.gene06672:CDS | 35.0% |
| ! | GATTCTACTGGTTATTGGAA+TGG | + | chr3.3:76662406-76662425 | MS.gene06672:CDS | 35.0% |
| ! | GCAAAACTTGTAGAAGGTAT+TGG | + | chr3.3:76661738-76661757 | MS.gene06672:intron | 35.0% |
| ! | GCTTTGCAAGATTTTAAGCT+TGG | + | chr3.3:76662175-76662194 | MS.gene06672:CDS | 35.0% |
| ! | GCTTTTCTCTCTAACATAGA+AGG | - | chr3.3:76661614-76661633 | None:intergenic | 35.0% |
| ! | TTTAAGCTTGGGGCTAATTT+TGG | + | chr3.3:76662187-76662206 | MS.gene06672:CDS | 35.0% |
| !! | ATCAAGCTTTAAACTGTCCA+AGG | + | chr3.3:76661648-76661667 | MS.gene06672:CDS | 35.0% |
| !!! | CAAGTTTTGCAAAAGTACCT+TGG | - | chr3.3:76661728-76661747 | None:intergenic | 35.0% |
| !!! | GGGCTAATTTTGGTTTAGAA+GGG | + | chr3.3:76662197-76662216 | MS.gene06672:CDS | 35.0% |
| AGAACTCAAGGTGATTCTAC+TGG | + | chr3.3:76662394-76662413 | MS.gene06672:CDS | 40.0% | |
| CAAAACCCTAAGATCCATCA+AGG | + | chr3.3:76661941-76661960 | MS.gene06672:CDS | 40.0% | |
| CTAGACTCAGACAAAGTCAT+AGG | - | chr3.3:76662130-76662149 | None:intergenic | 40.0% | |
| GAAGGGTTTGAGAATGGTTA+TGG | + | chr3.3:76662214-76662233 | MS.gene06672:CDS | 40.0% | |
| GGTTAAGATCTTGTCCTTGA+TGG | - | chr3.3:76661958-76661977 | None:intergenic | 40.0% | |
| GGTTGTGATTGTGAGATTGA+AGG | - | chr3.3:76661842-76661861 | None:intergenic | 40.0% | |
| GGTTTAGAAGGGTTTGAGAA+TGG | + | chr3.3:76662208-76662227 | MS.gene06672:CDS | 40.0% | |
| GTGTTAGTTGAATTGCACCT+TGG | - | chr3.3:76661668-76661687 | None:intergenic | 40.0% | |
| TATTGGAATGGAATGTTCGG+TGG | + | chr3.3:76662418-76662437 | MS.gene06672:CDS | 40.0% | |
| TTATCCACCACAAACAACTG+AGG | + | chr3.3:76661982-76662001 | MS.gene06672:CDS | 40.0% | |
| TTATCCTCAGTTGTTTGTGG+TGG | - | chr3.3:76661989-76662008 | None:intergenic | 40.0% | |
| ! | AATAATCAAGAGGGTTCTGG+TGG | + | chr3.3:76662253-76662272 | MS.gene06672:CDS | 40.0% |
| ! | CTCTCTAACATAGAAGGTGT+TGG | - | chr3.3:76661608-76661627 | None:intergenic | 40.0% |
| ! | CTTGTCCTTGATGGATCTTA+GGG | - | chr3.3:76661949-76661968 | None:intergenic | 40.0% |
| ! | GATTGAAGGTGATGATGATG+AGG | - | chr3.3:76661828-76661847 | None:intergenic | 40.0% |
| ! | TCTTGTCCTTGATGGATCTT+AGG | - | chr3.3:76661950-76661969 | None:intergenic | 40.0% |
| ! | TTTTGTGCTGTAGCAAGATC+AGG | - | chr3.3:76661899-76661918 | None:intergenic | 40.0% |
| !! | AAAAGTACCTTGGTTGTGAG+AGG | - | chr3.3:76661718-76661737 | None:intergenic | 40.0% |
| !! | TGTAGAAGGTATTGGACTGA+AGG | + | chr3.3:76661746-76661765 | MS.gene06672:intron | 40.0% |
| !! | TTGTTTGTGGTGGATAAGCT+AGG | - | chr3.3:76661979-76661998 | None:intergenic | 40.0% |
| !!! | GGGGCTAATTTTGGTTTAGA+AGG | + | chr3.3:76662196-76662215 | MS.gene06672:CDS | 40.0% |
| !!! | GGTTTTGAGAAGCAGATTGA+GGG | - | chr3.3:76661928-76661947 | None:intergenic | 40.0% |
| ACTATAGCCTCTCACAACCA+AGG | + | chr3.3:76661708-76661727 | MS.gene06672:intron | 45.0% | |
| AGAAATGTTCCTGTAGGTGG+TGG | + | chr3.3:76661779-76661798 | MS.gene06672:intron | 45.0% | |
| GGGTTTGAGAATGGTTATGG+TGG | + | chr3.3:76662217-76662236 | MS.gene06672:CDS | 45.0% | |
| TTTCTTGAACCACCACCTAC+AGG | - | chr3.3:76661791-76661810 | None:intergenic | 45.0% | |
| ! | AATGGTTATGGTGGCTTGCA+AGG | + | chr3.3:76662226-76662245 | MS.gene06672:CDS | 45.0% |
| ! | ATGGTTATGGTGGCTTGCAA+GGG | + | chr3.3:76662227-76662246 | MS.gene06672:CDS | 45.0% |
| !! | AGAAGGTATTGGACTGAAGG+TGG | + | chr3.3:76661749-76661768 | MS.gene06672:intron | 45.0% |
| !!! | GGGTTTTGAGAAGCAGATTG+AGG | - | chr3.3:76661929-76661948 | None:intergenic | 45.0% |
| ! | AATCAAGAGGGTTCTGGTGG+TGG | + | chr3.3:76662256-76662275 | MS.gene06672:CDS | 50.0% |
| ! | GAATGTTCGGTGGAGGATCA+TGG | + | chr3.3:76662428-76662447 | MS.gene06672:CDS | 50.0% |
| ! | TGGAATGGAATGTTCGGTGG+AGG | + | chr3.3:76662421-76662440 | MS.gene06672:CDS | 50.0% |
| !! | CAAGAGGGTTCTGGTGGTGG+TGG | + | chr3.3:76662259-76662278 | MS.gene06672:CDS | 60.0% |
| !! | GAGGGTTCTGGTGGTGGTGG+TGG | + | chr3.3:76662262-76662281 | MS.gene06672:CDS | 65.0% |
| !! | GGTTCTGGTGGTGGTGGTGG+TGG | + | chr3.3:76662265-76662284 | MS.gene06672:CDS | 65.0% |
| !! | GTGGTGGTGGTGGTGGTGCA+AGG | + | chr3.3:76662281-76662300 | MS.gene06672:CDS | 65.0% |
| !! | TCTGGTGGTGGTGGTGGTGG+TGG | + | chr3.3:76662268-76662287 | MS.gene06672:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTGG+TGG | + | chr3.3:76662271-76662290 | MS.gene06672:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 76661578 | 76662453 | 76661578 | ID=MS.gene06672 |
| chr3.3 | mRNA | 76661578 | 76662453 | 76661578 | ID=MS.gene06672.t1;Parent=MS.gene06672 |
| chr3.3 | exon | 76661578 | 76661669 | 76661578 | ID=MS.gene06672.t1.exon1;Parent=MS.gene06672.t1 |
| chr3.3 | CDS | 76661578 | 76661669 | 76661578 | ID=cds.MS.gene06672.t1;Parent=MS.gene06672.t1 |
| chr3.3 | exon | 76661751 | 76661794 | 76661751 | ID=MS.gene06672.t1.exon2;Parent=MS.gene06672.t1 |
| chr3.3 | CDS | 76661751 | 76661794 | 76661751 | ID=cds.MS.gene06672.t1;Parent=MS.gene06672.t1 |
| chr3.3 | exon | 76661912 | 76662453 | 76661912 | ID=MS.gene06672.t1.exon3;Parent=MS.gene06672.t1 |
| chr3.3 | CDS | 76661912 | 76662453 | 76661912 | ID=cds.MS.gene06672.t1;Parent=MS.gene06672.t1 |
| Gene Sequence |
| Protein sequence |
