AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066752

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066752.t1 MTR_4g007830 44.528 265 104 6 163 393 85 340 8.76e-65 211
MS.gene066752.t1 MTR_8g089760 51.235 162 72 3 234 393 1 157 5.94e-50 167
MS.gene066752.t1 MTR_1g009820 57.143 98 38 1 310 407 1 94 6.81e-35 125
MS.gene066752.t1 MTR_4g040020 32.544 169 106 3 223 388 11 174 2.61e-26 105
MS.gene066752.t1 MTR_5g022270 33.918 171 104 5 221 388 223 387 1.08e-22 100
MS.gene066752.t1 MTR_2g022080 33.333 171 105 5 221 388 223 387 2.64e-22 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTATCGAAAGCGATTTA+TGG 0.217435 2.2:-28466040 MS.gene066752:CDS
TCAAGTTTCAAATCAACTTA+TGG 0.227227 2.2:-28465637 MS.gene066752:CDS
AACCAATATTTAGGATAAAA+TGG 0.269706 2.2:+28466210 None:intergenic
GAATCTCGCTCGAAATTAAT+TGG 0.291467 2.2:-28466786 MS.gene066752:CDS
ATTGTTTCAGGTAATTGATT+TGG 0.299049 2.2:-28466271 MS.gene066752:intron
AATTGCTCATGTCGAAAGTT+TGG 0.300899 2.2:-28466988 MS.gene066752:CDS
TAGGAATGATTGTGCGATTT+GGG 0.304560 2.2:-28465445 MS.gene066752:CDS
TAATCAAACAAATGAGGATT+TGG 0.312634 2.2:-28467601 MS.gene066752:CDS
TTTCATGGAAAACTTGTTGA+TGG 0.314872 2.2:-28465683 MS.gene066752:CDS
ATCAAATGGATGATGGAAAA+AGG 0.314997 2.2:-28465420 MS.gene066752:CDS
GTCGGAATAAACCAATATTT+AGG 0.320561 2.2:+28466201 None:intergenic
AAATCCTCATTTGTTTGATT+AGG 0.320853 2.2:+28467603 None:intergenic
CTTGATACTTGTCTTCCTCT+TGG 0.328648 2.2:+28465803 None:intergenic
CTACTAAAACATTACCTCAT+AGG 0.337049 2.2:+28467554 None:intergenic
ATTGTAACCAAAACACCTTT+AGG 0.341831 2.2:-28466837 MS.gene066752:CDS
TTAGGAATGATTGTGCGATT+TGG 0.345924 2.2:-28465446 MS.gene066752:intron
GAAGACAAGTATCAAGAAAA+TGG 0.346177 2.2:-28465796 MS.gene066752:intron
TTTGTAACCTAAAGGTGTTT+TGG 0.354281 2.2:+28466830 None:intergenic
GGTTATCGAAAGCGATTTAT+GGG 0.356200 2.2:-28466039 MS.gene066752:CDS
CTGCACAAAATCAGAGTGTT+AGG 0.365999 2.2:-28466896 MS.gene066752:CDS
TGACATCGGCAGAAAGGAAT+TGG 0.366356 2.2:-28465862 MS.gene066752:CDS
GTTAGCACAAAGGATGAAAA+TGG 0.367000 2.2:-28467096 MS.gene066752:CDS
TTGATACTTGTCTTCCTCTT+GGG 0.380492 2.2:+28465804 None:intergenic
GGATTTGATCTTGCGGAATT+CGG 0.384621 2.2:-28465220 MS.gene066752:CDS
AAATTCTGTAATAGTGTTAT+CGG 0.391323 2.2:-28467751 MS.gene066752:CDS
GTTTATTCCGACCACATTAT+CGG 0.391757 2.2:-28466190 MS.gene066752:intron
ACAATCTGTAGATCAAATAA+AGG 0.392762 2.2:-28467048 MS.gene066752:CDS
TGAGCAATTTGTGAGCGTTT+TGG 0.431058 2.2:+28467002 None:intergenic
CGATACATGATACCGTTCAT+TGG 0.450387 2.2:-28465899 MS.gene066752:CDS
TTAGCAACTCGTGTTGGCAT+TGG 0.451963 2.2:-28466088 MS.gene066752:intron
TAGGGACACGAGCACCTCAT+TGG 0.461676 2.2:+28467573 None:intergenic
GTGCGATTTGGGTAATCAAA+TGG 0.465432 2.2:-28465434 MS.gene066752:CDS
TACTAAAACATTACCTCATA+GGG 0.479347 2.2:+28467555 None:intergenic
AGGAAACTCTTCTCAACTAA+AGG 0.490542 2.2:-28466876 MS.gene066752:CDS
ATGGATCCCTCTTTCTATGT+GGG 0.495254 2.2:-28465664 MS.gene066752:CDS
TTTGTTGCTCCACGTGGTTA+CGG 0.500598 2.2:-28465592 MS.gene066752:CDS
GATGGATCCCTCTTTCTATG+TGG 0.506410 2.2:-28465665 MS.gene066752:CDS
CTGTAATAGTGTTATCGGAC+AGG 0.508801 2.2:-28467746 MS.gene066752:intron
GCATCCTAATCAAACAAATG+AGG 0.512763 2.2:-28467607 MS.gene066752:CDS
TTGGGTAATCAAATGGATGA+TGG 0.515751 2.2:-28465427 MS.gene066752:CDS
TACAAATTAGATACCTCAAA+AGG 0.521430 2.2:-28466813 MS.gene066752:CDS
GTATCTAATTTGTAACCTAA+AGG 0.521441 2.2:+28466822 None:intergenic
CATAATTGACATCGGCAGAA+AGG 0.522205 2.2:-28465868 MS.gene066752:CDS
GATCCCTCTTTCTATGTGGG+TGG 0.554391 2.2:-28465661 MS.gene066752:CDS
TGAACCACCCACATAGAAAG+AGG 0.556566 2.2:+28465657 None:intergenic
GAGTGCTGAGTTTATTTCAA+AGG 0.557552 2.2:-28466013 MS.gene066752:intron
TTACACTAACAGGTGGATGA+AGG 0.560129 2.2:-28465297 MS.gene066752:intron
CCACGTGGTTACGGAAGTCA+AGG 0.569221 2.2:-28465583 MS.gene066752:CDS
ATCGAAAGCGATTTATGGGG+GGG 0.572674 2.2:-28466035 MS.gene066752:CDS
TGCCGATGTCAATTATGAGA+AGG 0.572917 2.2:+28465874 None:intergenic
CAATGAACGGTATCATGTAT+CGG 0.573628 2.2:+28465900 None:intergenic
TTAATAATTTACCGATAATG+TGG 0.589365 2.2:+28466179 None:intergenic
ATTTATTTAGCAACTCGTGT+TGG 0.592550 2.2:-28466094 MS.gene066752:intron
TTATCGAAAGCGATTTATGG+GGG 0.592691 2.2:-28466037 MS.gene066752:CDS
AAGGATGAAAATGGTAGAGA+CGG 0.599188 2.2:-28467087 MS.gene066752:CDS
TAATTTACCGATAATGTGGT+CGG 0.599623 2.2:+28466183 None:intergenic
ATTAGATACCTCAAAAGGCA+AGG 0.605997 2.2:-28466808 MS.gene066752:CDS
TGGTGGAGATGAAACGGAAT+CGG 0.606779 2.2:+28467821 None:intergenic
AATTGGAGCTAATATTCCAA+AGG 0.615507 2.2:-28466769 MS.gene066752:intron
TATCGAAAGCGATTTATGGG+GGG 0.620517 2.2:-28466036 MS.gene066752:CDS
AAAAGAGGGATTTGATCTTG+CGG 0.622365 2.2:-28465227 MS.gene066752:CDS
TTCCTTCTCATAATTGACAT+CGG 0.629925 2.2:-28465876 MS.gene066752:CDS
GAGGTGCTCGTGTCCCTATG+AGG 0.630432 2.2:-28467568 MS.gene066752:intron
AGGATTTGGAATTTCCAATG+AGG 0.632110 2.2:-28467587 MS.gene066752:CDS
TTTGGTTACAATAATCGGAG+TGG 0.642068 2.2:+28466848 None:intergenic
GTTATCGAAAGCGATTTATG+GGG 0.643499 2.2:-28466038 MS.gene066752:CDS
TATGAGAAGGAACCAATGAA+CGG 0.646603 2.2:+28465887 None:intergenic
TTTAAGTTTGTTGCTCCACG+TGG 0.650344 2.2:-28465598 MS.gene066752:CDS
GTTCGTCAGCTCTTCCCAAG+AGG 0.652918 2.2:-28465818 MS.gene066752:CDS
GAACCACCCACATAGAAAGA+GGG 0.662397 2.2:+28465658 None:intergenic
CCTTGACTTCCGTAACCACG+TGG 0.696038 2.2:+28465583 None:intergenic
CACGTGGTTACGGAAGTCAA+GGG 0.703622 2.2:-28465582 MS.gene066752:CDS
TATGAGAAAGAACATAGACA+TGG 0.713561 2.2:-28466927 MS.gene066752:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAACAAATATTCT+TGG + chr2.2:28465825-28465844 None:intergenic 10.0%
!!! ATACTCATAATTTTTTATTT+AGG - chr2.2:28467612-28467631 MS.gene066752:CDS 10.0%
!! ATATATATATATATCATGTG+TGG - chr2.2:28465594-28465613 MS.gene066752:CDS 15.0%
!! AAATTCTGTAATAGTGTTAT+CGG - chr2.2:28465325-28465344 MS.gene066752:intron 20.0%
!! ATTTCTCTTTAAATTGTTTC+AGG - chr2.2:28466793-28466812 MS.gene066752:CDS 20.0%
!! CTACAATTATATGAAGAATA+TGG + chr2.2:28466339-28466358 None:intergenic 20.0%
!! TAATTAATCTTAATTGCTGT+AGG - chr2.2:28466603-28466622 MS.gene066752:intron 20.0%
!! TTAATAATTTACCGATAATG+TGG + chr2.2:28466900-28466919 None:intergenic 20.0%
!!! AACCAATATTTAGGATAAAA+TGG + chr2.2:28466869-28466888 None:intergenic 20.0%
!!! TTGTTATCATTTTGTTTTGA+TGG + chr2.2:28465278-28465297 None:intergenic 20.0%
!!! TTTTTCTTTTCTTCATTCAA+AGG + chr2.2:28465308-28465327 None:intergenic 20.0%
!!! TTTTTTATATCTTACGAGTT+TGG + chr2.2:28467520-28467539 None:intergenic 20.0%
! AAATCCTCATTTGTTTGATT+AGG + chr2.2:28465476-28465495 None:intergenic 25.0%
! AAATGATGAAGAATCTAATC+TGG - chr2.2:28466446-28466465 MS.gene066752:intron 25.0%
! AAGAATATGGTTAATACCTT+TGG + chr2.2:28466326-28466345 None:intergenic 25.0%
! ACAATCTGTAGATCAAATAA+AGG - chr2.2:28466028-28466047 MS.gene066752:CDS 25.0%
! ACCAACAGAAAAAATGATAA+AGG - chr2.2:28467015-28467034 MS.gene066752:CDS 25.0%
! ATCATCGTCAATAAATTTGT+TGG + chr2.2:28466699-28466718 None:intergenic 25.0%
! CATAGCTATGTAGATAAAAA+GGG + chr2.2:28467122-28467141 None:intergenic 25.0%
! CCAACAGAAAAAATGATAAA+GGG - chr2.2:28467016-28467035 MS.gene066752:CDS 25.0%
! TAAACAAAAATTCCAACAGA+GGG + chr2.2:28465852-28465871 None:intergenic 25.0%
! TAATCAAACAAATGAGGATT+TGG - chr2.2:28465475-28465494 MS.gene066752:intron 25.0%
! TACAAATTAGATACCTCAAA+AGG - chr2.2:28466263-28466282 MS.gene066752:CDS 25.0%
! TACTAAAACATTACCTCATA+GGG + chr2.2:28465524-28465543 None:intergenic 25.0%
! TCAAGTTTCAAATCAACTTA+TGG - chr2.2:28467439-28467458 MS.gene066752:intron 25.0%
! TCATAGCTATGTAGATAAAA+AGG + chr2.2:28467123-28467142 None:intergenic 25.0%
! TTAAACAAAAATTCCAACAG+AGG + chr2.2:28465853-28465872 None:intergenic 25.0%
! TTATATGTTTGTTTATGCAG+TGG - chr2.2:28466385-28466404 MS.gene066752:intron 25.0%
!! AAATCCCTCTTTTTATTGTA+AGG + chr2.2:28467842-28467861 None:intergenic 25.0%
!! ATTGTTTCAGGTAATTGATT+TGG - chr2.2:28466805-28466824 MS.gene066752:CDS 25.0%
!! CACCATTTTATCCTAAATAT+TGG - chr2.2:28466864-28466883 MS.gene066752:CDS 25.0%
!! GTATCTAATTTGTAACCTAA+AGG + chr2.2:28466257-28466276 None:intergenic 25.0%
!! TCATCGTCAATAAATTTGTT+GGG + chr2.2:28466698-28466717 None:intergenic 25.0%
!!! ACATTTTTAGTCTCTTTTCA+TGG - chr2.2:28467378-28467397 MS.gene066752:intron 25.0%
!!! ATTTTAATGTATGCTCATTG+TGG - chr2.2:28466949-28466968 MS.gene066752:CDS 25.0%
!!! CAGCTTTTTGATTATATTGA+TGG + chr2.2:28466488-28466507 None:intergenic 25.0%
!!! TTATCATTTTGTTTTGATGG+TGG + chr2.2:28465275-28465294 None:intergenic 25.0%
AATCTGGCAGTTTACATATT+TGG - chr2.2:28466462-28466481 MS.gene066752:intron 30.0%
AATTGGAGCTAATATTCCAA+AGG - chr2.2:28466307-28466326 MS.gene066752:intron 30.0%
ATTGTAACCAAAACACCTTT+AGG - chr2.2:28466239-28466258 MS.gene066752:CDS 30.0%
ATTTATTTAGCAACTCGTGT+TGG - chr2.2:28466982-28467001 MS.gene066752:CDS 30.0%
CATGTACACATTAGTGATAA+TGG - chr2.2:28465742-28465761 MS.gene066752:intron 30.0%
CTACTAAAACATTACCTCAT+AGG + chr2.2:28465525-28465544 None:intergenic 30.0%
GAAGACAAGTATCAAGAAAA+TGG - chr2.2:28467280-28467299 MS.gene066752:intron 30.0%
GATCTGTTAGCACAATTTAA+TGG - chr2.2:28465631-28465650 MS.gene066752:CDS 30.0%
GCATCCTTACAATAAAAAGA+GGG - chr2.2:28467835-28467854 MS.gene066752:CDS 30.0%
GTCGGAATAAACCAATATTT+AGG + chr2.2:28466878-28466897 None:intergenic 30.0%
TAATTTACCGATAATGTGGT+CGG + chr2.2:28466896-28466915 None:intergenic 30.0%
TATGAGAAAGAACATAGACA+TGG - chr2.2:28466149-28466168 MS.gene066752:intron 30.0%
TGCATCCTTACAATAAAAAG+AGG - chr2.2:28467834-28467853 MS.gene066752:CDS 30.0%
TTCCTTCTCATAATTGACAT+CGG - chr2.2:28467200-28467219 MS.gene066752:intron 30.0%
TTTACCACAATTACACTAAC+AGG - chr2.2:28467769-28467788 MS.gene066752:CDS 30.0%
! TTCATCATTTAACTTCAGTG+TGG + chr2.2:28466436-28466455 None:intergenic 30.0%
!! AATTTAATGGTGGATTTGGT+AGG - chr2.2:28465644-28465663 MS.gene066752:CDS 30.0%
!! ATCAAATGGATGATGGAAAA+AGG - chr2.2:28467656-28467675 MS.gene066752:intron 30.0%
!! TTTAACTTCAGTGTGGTTTT+AGG + chr2.2:28466429-28466448 None:intergenic 30.0%
!! TTTCATGGAAAACTTGTTGA+TGG - chr2.2:28467393-28467412 MS.gene066752:intron 30.0%
!!! AATTTTGCGAAAGTTTGCTA+AGG - chr2.2:28466115-28466134 MS.gene066752:intron 30.0%
!!! CACACTTCTTATTTTTACAG+AGG - chr2.2:28465958-28465977 MS.gene066752:intron 30.0%
!!! CCCTTTATCATTTTTTCTGT+TGG + chr2.2:28467019-28467038 None:intergenic 30.0%
!!! CTATGAGGTAATGTTTTAGT+AGG - chr2.2:28465523-28465542 MS.gene066752:intron 30.0%
!!! CTTATGTCATTTTTTGTGTG+TGG - chr2.2:28465397-28465416 MS.gene066752:intron 30.0%
!!! GGTGTTTTGGTTACAATAAT+CGG + chr2.2:28466236-28466255 None:intergenic 30.0%
!!! TTTGTAACCTAAAGGTGTTT+TGG + chr2.2:28466249-28466268 None:intergenic 30.0%
!!! TTTTTTTTCTTTCCCTCTGT+TGG - chr2.2:28465837-28465856 MS.gene066752:CDS 30.0%
AAAAGAGGGATTTGATCTTG+CGG - chr2.2:28467849-28467868 MS.gene066752:CDS 35.0%
AAGGATGAAAATGGTAGAGA+CGG - chr2.2:28465989-28466008 MS.gene066752:intron 35.0%
AATTGCTCATGTCGAAAGTT+TGG - chr2.2:28466088-28466107 MS.gene066752:intron 35.0%
ACAGAGATAGATTACGTTGA+AGG - chr2.2:28466644-28466663 MS.gene066752:intron 35.0%
AGGAAACTCTTCTCAACTAA+AGG - chr2.2:28466200-28466219 MS.gene066752:CDS 35.0%
ATTAGATACCTCAAAAGGCA+AGG - chr2.2:28466268-28466287 MS.gene066752:intron 35.0%
CAATGAACGGTATCATGTAT+CGG + chr2.2:28467179-28467198 None:intergenic 35.0%
CTGTTAGCACAATTTAATGG+TGG - chr2.2:28465634-28465653 MS.gene066752:CDS 35.0%
GAATCTCGCTCGAAATTAAT+TGG - chr2.2:28466290-28466309 MS.gene066752:intron 35.0%
GCATCCTAATCAAACAAATG+AGG - chr2.2:28465469-28465488 MS.gene066752:intron 35.0%
GGTTATCGAAAGCGATTTAT+GGG - chr2.2:28467037-28467056 MS.gene066752:CDS 35.0%
GTTAGCACAAAGGATGAAAA+TGG - chr2.2:28465980-28465999 MS.gene066752:intron 35.0%
GTTATCGAAAGCGATTTATG+GGG - chr2.2:28467038-28467057 MS.gene066752:CDS 35.0%
GTTTATTCCGACCACATTAT+CGG - chr2.2:28466886-28466905 MS.gene066752:CDS 35.0%
TAGATTACGTTGAAGGAACT+AGG - chr2.2:28466651-28466670 MS.gene066752:intron 35.0%
TAGGAATGATTGTGCGATTT+GGG - chr2.2:28467631-28467650 MS.gene066752:CDS 35.0%
TATGAGAAGGAACCAATGAA+CGG + chr2.2:28467192-28467211 None:intergenic 35.0%
TTAGGAATGATTGTGCGATT+TGG - chr2.2:28467630-28467649 MS.gene066752:CDS 35.0%
TTATCGAAAGCGATTTATGG+GGG - chr2.2:28467039-28467058 MS.gene066752:CDS 35.0%
TTGATACTTGTCTTCCTCTT+GGG + chr2.2:28467275-28467294 None:intergenic 35.0%
TTGGGTAATCAAATGGATGA+TGG - chr2.2:28467649-28467668 MS.gene066752:CDS 35.0%
TTTGGTTACAATAATCGGAG+TGG + chr2.2:28466231-28466250 None:intergenic 35.0%
! AGGATTTGGAATTTCCAATG+AGG - chr2.2:28465489-28465508 MS.gene066752:intron 35.0%
! ATCATGTGTGGATTGCTTAA+TGG - chr2.2:28465606-28465625 MS.gene066752:CDS 35.0%
! TTTTACAGAGGTTAGCACAA+AGG - chr2.2:28465970-28465989 MS.gene066752:intron 35.0%
!! GAGTGCTGAGTTTATTTCAA+AGG - chr2.2:28467063-28467082 MS.gene066752:CDS 35.0%
!! GCACAATTTAATGGTGGATT+TGG - chr2.2:28465640-28465659 MS.gene066752:CDS 35.0%
!!! ATGTCATTTTTTGTGTGTGG+TGG - chr2.2:28465400-28465419 MS.gene066752:CDS 35.0%
!!! TTTTGATGGTGGAGATGAAA+CGG + chr2.2:28465264-28465283 None:intergenic 35.0%
ACCACAATTACACTAACAGG+TGG - chr2.2:28467772-28467791 MS.gene066752:CDS 40.0%
ATGGATCCCTCTTTCTATGT+GGG - chr2.2:28467412-28467431 MS.gene066752:intron 40.0%
CATAATTGACATCGGCAGAA+AGG - chr2.2:28467208-28467227 MS.gene066752:intron 40.0%
CGATACATGATACCGTTCAT+TGG - chr2.2:28467177-28467196 MS.gene066752:intron 40.0%
CTGTAATAGTGTTATCGGAC+AGG - chr2.2:28465330-28465349 MS.gene066752:intron 40.0%
CTTGATACTTGTCTTCCTCT+TGG + chr2.2:28467276-28467295 None:intergenic 40.0%
GGATTTGATCTTGCGGAATT+CGG - chr2.2:28467856-28467875 MS.gene066752:CDS 40.0%
GGGTTATCGAAAGCGATTTA+TGG - chr2.2:28467036-28467055 MS.gene066752:CDS 40.0%
TATCGAAAGCGATTTATGGG+GGG - chr2.2:28467040-28467059 MS.gene066752:CDS 40.0%
TCCACCTGTTAGTGTAATTG+TGG + chr2.2:28467776-28467795 None:intergenic 40.0%
TCTTAATTGCTGTAGGCAAG+AGG - chr2.2:28466610-28466629 MS.gene066752:intron 40.0%
TGCCGATGTCAATTATGAGA+AGG + chr2.2:28467205-28467224 None:intergenic 40.0%
TTACACTAACAGGTGGATGA+AGG - chr2.2:28467779-28467798 MS.gene066752:CDS 40.0%
! CTGCACAAAATCAGAGTGTT+AGG - chr2.2:28466180-28466199 MS.gene066752:intron 40.0%
! GTGCGATTTGGGTAATCAAA+TGG - chr2.2:28467642-28467661 MS.gene066752:CDS 40.0%
! GTGGATTTGGTAGGAACTAA+TGG - chr2.2:28465653-28465672 MS.gene066752:CDS 40.0%
! TGAGCAATTTGTGAGCGTTT+TGG + chr2.2:28466077-28466096 None:intergenic 40.0%
!! TTTAAGTTTGTTGCTCCACG+TGG - chr2.2:28467478-28467497 MS.gene066752:intron 40.0%
ATCGAAAGCGATTTATGGGG+GGG - chr2.2:28467041-28467060 MS.gene066752:CDS 45.0%
GAACCACCCACATAGAAAGA+GGG + chr2.2:28467421-28467440 None:intergenic 45.0%
GATGGATCCCTCTTTCTATG+TGG - chr2.2:28467411-28467430 MS.gene066752:intron 45.0%
TGAACCACCCACATAGAAAG+AGG + chr2.2:28467422-28467441 None:intergenic 45.0%
TGACATCGGCAGAAAGGAAT+TGG - chr2.2:28467214-28467233 MS.gene066752:intron 45.0%
TGGTGGAGATGAAACGGAAT+CGG + chr2.2:28465258-28465277 None:intergenic 45.0%
TTTGTTGCTCCACGTGGTTA+CGG - chr2.2:28467484-28467503 MS.gene066752:intron 45.0%
! TTAGCAACTCGTGTTGGCAT+TGG - chr2.2:28466988-28467007 MS.gene066752:CDS 45.0%
!! TAAATAACAATTAAAATAAA+AGG - chr2.2:28465888-28465907 MS.gene066752:CDS 5.0%
!!! TAAATATTATCTTATTTTAT+AGG - chr2.2:28466563-28466582 MS.gene066752:intron 5.0%
AGGAACTAGGAAGTTGCTGC+AGG - chr2.2:28466664-28466683 MS.gene066752:intron 50.0%
CACGTGGTTACGGAAGTCAA+GGG - chr2.2:28467494-28467513 MS.gene066752:intron 50.0%
GATCCCTCTTTCTATGTGGG+TGG - chr2.2:28467415-28467434 MS.gene066752:intron 50.0%
GGAACTAGGAAGTTGCTGCA+GGG - chr2.2:28466665-28466684 MS.gene066752:intron 50.0%
! GCGAGATTCCTTGCCTTTTG+AGG + chr2.2:28466279-28466298 None:intergenic 50.0%
CCACGTGGTTACGGAAGTCA+AGG - chr2.2:28467493-28467512 MS.gene066752:intron 55.0%
CCTTGACTTCCGTAACCACG+TGG + chr2.2:28467496-28467515 None:intergenic 55.0%
GTTCGTCAGCTCTTCCCAAG+AGG - chr2.2:28467258-28467277 MS.gene066752:intron 55.0%
TAGGGACACGAGCACCTCAT+TGG + chr2.2:28465506-28465525 None:intergenic 55.0%
! GAGGTGCTCGTGTCCCTATG+AGG - chr2.2:28465508-28465527 MS.gene066752:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 28465206 28467892 28465206 ID=MS.gene066752
chr2.2 mRNA 28465206 28467892 28465206 ID=MS.gene066752.t1;Parent=MS.gene066752
chr2.2 exon 28467747 28467892 28467747 ID=MS.gene066752.t1.exon1;Parent=MS.gene066752.t1
chr2.2 CDS 28467747 28467892 28467747 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28467569 28467668 28467569 ID=MS.gene066752.t1.exon2;Parent=MS.gene066752.t1
chr2.2 CDS 28467569 28467668 28467569 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28466770 28467120 28466770 ID=MS.gene066752.t1.exon3;Parent=MS.gene066752.t1
chr2.2 CDS 28466770 28467120 28466770 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28466191 28466283 28466191 ID=MS.gene066752.t1.exon4;Parent=MS.gene066752.t1
chr2.2 CDS 28466191 28466283 28466191 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28466014 28466106 28466014 ID=MS.gene066752.t1.exon5;Parent=MS.gene066752.t1
chr2.2 CDS 28466014 28466106 28466014 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28465797 28465931 28465797 ID=MS.gene066752.t1.exon6;Parent=MS.gene066752.t1
chr2.2 CDS 28465797 28465931 28465797 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28465574 28465710 28465574 ID=MS.gene066752.t1.exon7;Parent=MS.gene066752.t1
chr2.2 CDS 28465574 28465710 28465574 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28465398 28465464 28465398 ID=MS.gene066752.t1.exon8;Parent=MS.gene066752.t1
chr2.2 CDS 28465398 28465464 28465398 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
chr2.2 exon 28465206 28465307 28465206 ID=MS.gene066752.t1.exon9;Parent=MS.gene066752.t1
chr2.2 CDS 28465206 28465307 28465206 ID=cds.MS.gene066752.t1;Parent=MS.gene066752.t1
Gene Sequence

>MS.gene066752

ATGGGTAACAAAAGAAAACGATCACAAAATGACAATGAAGAAAGATCAACCGATTCCGTTTCATCTCCACCATCAAAACAAAATGATAACAATAAAAAACCTTTGAATGAAGAAAAGAAAAATTCTGTAATAGTGTTATCGGACAGGTATACAATTTATTACTTTATTTTATGAAATTGTTTGATAAAGTGCTTATGTCATTTTTTGTGTGTGGTGGTTTATAGTATCAAAGAAGCAGACAATCTGAAATATTTTTATGATTTGCATCCTAATCAAACAAATGAGGATTTGGAATTTCCAATGAGGTGCTCGTGTCCCTATGAGGTAATGTTTTAGTAGGCATCTTATTTGTTTCTATGAGTTAATATATATTTCATGTGTGTTACTAATATATATATATATCATGTGTGGATTGCTTAATGGTCGATCTGTTAGCACAATTTAATGGTGGATTTGGTAGGAACTAATGGTGTAATTTTTATGATCAGTTCTGTAATTTTTATGTAACAGATTTTTATAAAGACTACAATATAGTTCATGTACACATTAGTGATAATGGTTTGTATATAGTGATAATTGTTCATGTATACATTATTGTATTAAAAATCATATTTAGCCAAGAATATTTGTTTTTTTTTTCTTTCCCTCTGTTGGAATTTTTGTTTAAGAATGTAAAATAATGTAAATAACAATTAAAATAAAAGGAAAAATAAACTATTATTTATTATTCTGTTACTGCACATTTTGTTTTACACACTTCTTATTTTTACAGAGGTTAGCACAAAGGATGAAAATGGTAGAGACGGTTGTTTACAGCACAAAACAATCTGTAGATCAAATAAAGGAAATACTTGTGAAGAGCGCTACACCAAAACGCTCACAAATTGCTCATGTCGAAAGTTTGGAAGAAATTTTGCGAAAGTTTGCTAAGGAAAAAGATTCATATGAGAAAGAACATAGACATGGCAAAGAATCTGCACAAAATCAGAGTGTTAGGAAACTCTTCTCAACTAAAGGAGACACCACTCCGATTATTGTAACCAAAACACCTTTAGGTTACAAATTAGATACCTCAAAAGGCAAGGAATCTCGCTCGAAATTAATTGGAGCTAATATTCCAAAGGTATTAACCATATTCTTCATATAATTGTAGTTTCTTTACATTTTATTTAATTTATTTTATATGTTTGTTTATGCAGTGGCTGACTGAATTATATCATCCTAAAACCACACTGAAGTTAAATGATGAAGAATCTAATCTGGCAGTTTACATATTTGGCCATCAATATAATCAAAAAGCTGATGAGTTAATTATACTTTTTATTTTATTTTGACTTTTTTTATTATTATAATTTTTTAAATATTATCTTATTTTATAGGTTGTACATATAATGATATAATTAATCTTAATTGCTGTAGGCAAGAGGTCATGATCAGTACAGAGATAGATTACGTTGAAGGAACTAGGAAGTTGCTGCAGGGTTTGAAACCCAACAAATTTATTGACGATGATGTACAAACTAGTTTCTTTTTCAATATTGAATTGATTTTATACTTAAATCTCTAACGTTAAATTATAAGATAATAATTTCTCTTTAAATTGTTTCAGGTAATTGATTTGGCTGCTTGCATGTTGACTTTTGATGAGAGTCTGAAATCACCATTTTATCCTAAATATTGGTTTATTCCGACCACATTATCGGTAAATTATTAATGATAAATATTTTTTATGTAAAAGTTACAATTTTAATGTATGCTCATTGTGGTTTAAAATGTATTTATTTAGCAACTCGTGTTGGCATTGGAAACACCAACAGAAAAAATGATAAAGGGTTATCGAAAGCGATTTATGGGGGGGAGTGCTGAGTTTATTTCAAAGGTAATTAACATGTATTTTTATAACACTTACAAATCCCTTTTTATCTACATAGCTATGATACTAATATTTTGAATATAATCAGATATTTGTGCCGATACATGATACCGTTCATTGGTTCCTTCTCATAATTGACATCGGCAGAAAGGAATTGGTTTATCTTGACTCGTATCAAAGTTCGTCAGCTCTTCCCAAGAGGAAGACAAGTATCAAGAAAATGGTATGAAAAATACATTTTATAGTTTATATCTTACAATTTCAACTATCATAGAAAATCACTAACAAATATAAATCATACATTTTTAGTCTCTTTTCATGGAAAACTTGTTGATGGATCCCTCTTTCTATGTGGGTGGTTCAAGTTTCAAATCAACTTATGGACACAAGATATCAGAGTTTAAGTTTGTTGCTCCACGTGGTTACGGAAGTCAAGGGCCAAACTCGTAAGATATAAAAAATATTTGTTTATCAAATTTTAGTTACTCTTAAAATTTCTACTCATAATTTTTTATTTTTTAATTTAAATAATAATACTCATAATTTTTTATTTAGGAATGATTGTGCGATTTGGGTAATCAAATGGATGATGGAAAAAGGAGCTGATGAATATAAGATTAAAGTATGCAAAAAAAATTGCTCAATTAAGTTTTCAAATTTATTACATTATATGCAATTGACACTACTGACTTTACCACAATTACACTAACAGGTGGATGAAGGCTCAAGACTACAAACTGCACTGCAATTGACAATGCATCCTTACAATAAAAAGAGGGATTTGATCTTGCGGAATTCGGTGAAATACAGATAA
Protein sequence

>MS.gene066752.t1

MGNKRKRSQNDNEERSTDSVSSPPSKQNDNNKKPLNEEKKNSVIVLSDSIKEADNLKYFYDLHPNQTNEDLEFPMRCSCPYERLAQRMKMVETVVYSTKQSVDQIKEILVKSATPKRSQIAHVESLEEILRKFAKEKDSYEKEHRHGKESAQNQSVRKLFSTKGDTTPIIVTKTPLGYKLDTSKGKESRSKLIGANIPKVIDLAACMLTFDESLKSPFYPKYWFIPTTLSQLVLALETPTEKMIKGYRKRFMGGSAEFISKIFVPIHDTVHWFLLIIDIGRKELVYLDSYQSSSALPKRKTSIKKMSLFMENLLMDPSFYVGGSSFKSTYGHKISEFKFVAPRGYGSQGPNSNDCAIWVIKWMMEKGADEYKIKVDEGSRLQTALQLTMHPYNKKRDLILRNSVKYR