Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066953.t1 | XP_013457391.1 | 100 | 57 | 0 | 0 | 4 | 60 | 1 | 57 | 4.40E-24 | 119.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066953.t1 | G8A1V0 | 100.0 | 57 | 0 | 0 | 4 | 60 | 1 | 57 | 3.2e-24 | 119.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene066953 | 0.802383 | 5.76E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene066953 | 0.812344 | 4.44E-51 | -1.69E-46 |
| MS.gene051063 | MS.gene066953 | 0.816498 | 5.36E-52 | -1.69E-46 |
| MS.gene051687 | MS.gene066953 | 0.804378 | 2.22E-49 | -1.69E-46 |
| MS.gene051746 | MS.gene066953 | 0.834904 | 2.29E-56 | -1.69E-46 |
| MS.gene051748 | MS.gene066953 | 0.82481 | 6.60E-54 | -1.69E-46 |
| MS.gene051756 | MS.gene066953 | 0.800447 | 1.44E-48 | -1.69E-46 |
| MS.gene051903 | MS.gene066953 | 0.84547 | 3.98E-59 | -1.69E-46 |
| MS.gene052947 | MS.gene066953 | 0.816193 | 6.27E-52 | -1.69E-46 |
| MS.gene053952 | MS.gene066953 | 0.851309 | 9.65E-61 | -1.69E-46 |
| MS.gene055424 | MS.gene066953 | 0.80018 | 1.63E-48 | -1.69E-46 |
| MS.gene056075 | MS.gene066953 | 0.80529 | 1.43E-49 | -1.69E-46 |
| MS.gene056601 | MS.gene066953 | 0.801012 | 1.10E-48 | -1.69E-46 |
| MS.gene056631 | MS.gene066953 | 0.801028 | 1.09E-48 | -1.69E-46 |
| MS.gene057094 | MS.gene066953 | 0.851828 | 6.88E-61 | -1.69E-46 |
| MS.gene058906 | MS.gene066953 | 0.819009 | 1.45E-52 | -1.69E-46 |
| MS.gene059171 | MS.gene066953 | 0.806283 | 8.87E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene066953 | 0.870281 | 1.64E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 8 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCAAGTTGTAGGATAA+TGG | 0.313664 | 8.4:+69170414 | None:intergenic |
| ATTTCAAGTTGTAGGATAAT+GGG | 0.372893 | 8.4:+69170415 | None:intergenic |
| AGGATAATGGGGCGGAAAGC+TGG | 0.409342 | 8.4:+69170427 | MS.gene066953:CDS |
| AAATACATGTATAGAATCTC+AGG | 0.487514 | 8.4:+69170582 | MS.gene066953:CDS |
| TAATGGGGCGGAAAGCTGGC+GGG | 0.509687 | 8.4:+69170431 | MS.gene066953:CDS |
| ACTTCATAAACCTTGCATGA+AGG | 0.526107 | 8.4:+69170483 | MS.gene066953:CDS |
| GATCATGTTTATGAACTGTA+TGG | 0.548690 | 8.4:+69170510 | MS.gene066953:CDS |
| ATGATCATTTCCTTCATGCA+AGG | 0.553694 | 8.4:-69170493 | None:intergenic |
| ATAATGGGGCGGAAAGCTGG+CGG | 0.563977 | 8.4:+69170430 | MS.gene066953:CDS |
| TGAAGCATGTGCTAAGCAGA+AGG | 0.631587 | 8.4:+69170552 | MS.gene066953:CDS |
| TTTCAAGTTGTAGGATAATG+GGG | 0.640667 | 8.4:+69170416 | None:intergenic |
| CAAGTTGTAGGATAATGGGG+CGG | 0.699621 | 8.4:+69170419 | None:intergenic |
| CACATGCTTCAACATCACTG+TGG | 0.742984 | 8.4:-69170540 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATCATGTTTATGAACTGTA+TGG | + | chr8.4:69170510-69170529 | MS.gene066953:CDS | 30.0% | |
| ACTTCATAAACCTTGCATGA+AGG | + | chr8.4:69170483-69170502 | MS.gene066953:CDS | 35.0% | |
| ATGATCATTTCCTTCATGCA+AGG | - | chr8.4:69170496-69170515 | None:intergenic | 35.0% | |
| CACATGCTTCAACATCACTG+TGG | - | chr8.4:69170543-69170562 | None:intergenic | 45.0% | |
| TGAAGCATGTGCTAAGCAGA+AGG | + | chr8.4:69170552-69170571 | MS.gene066953:CDS | 45.0% | |
| ! | ATAATGGGGCGGAAAGCTGG+CGG | + | chr8.4:69170430-69170449 | MS.gene066953:CDS | 55.0% |
| !! | AGGATAATGGGGCGGAAAGC+TGG | + | chr8.4:69170427-69170446 | MS.gene066953:CDS | 55.0% |
| TAATGGGGCGGAAAGCTGGC+GGG | + | chr8.4:69170431-69170450 | MS.gene066953:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 69170423 | 69170603 | 69170423 | ID=MS.gene066953 |
| chr8.4 | mRNA | 69170423 | 69170603 | 69170423 | ID=MS.gene066953.t1;Parent=MS.gene066953 |
| chr8.4 | exon | 69170423 | 69170603 | 69170423 | ID=MS.gene066953.t1.exon1;Parent=MS.gene066953.t1 |
| chr8.4 | CDS | 69170423 | 69170603 | 69170423 | ID=cds.MS.gene066953.t1;Parent=MS.gene066953.t1 |
| Gene Sequence |
| Protein sequence |
