Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067755.t1 | XP_024637633.1 | 97.1 | 170 | 5 | 0 | 1 | 170 | 1 | 170 | 1.80E-86 | 328.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067755.t1 | I3SF91 | 97.1 | 170 | 5 | 0 | 1 | 170 | 1 | 170 | 1.3e-86 | 328.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050128 | MS.gene067755 | 0.802192 | 6.30E-49 | -1.69E-46 |
| MS.gene050584 | MS.gene067755 | 0.82819 | 1.03E-54 | -1.69E-46 |
| MS.gene050585 | MS.gene067755 | 0.808196 | 3.49E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene067755 | 0.833352 | 5.60E-56 | -1.69E-46 |
| MS.gene052142 | MS.gene067755 | 0.812492 | 4.13E-51 | -1.69E-46 |
| MS.gene052178 | MS.gene067755 | 0.813052 | 3.11E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene067755 | 0.825703 | 4.06E-54 | -1.69E-46 |
| MS.gene054010 | MS.gene067755 | 0.813904 | 2.02E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene067755 | 0.819558 | 1.09E-52 | -1.69E-46 |
| MS.gene054012 | MS.gene067755 | 0.813078 | 3.07E-51 | -1.69E-46 |
| MS.gene054013 | MS.gene067755 | 0.815173 | 1.06E-51 | -1.69E-46 |
| MS.gene054214 | MS.gene067755 | 0.842252 | 2.90E-58 | -1.69E-46 |
| MS.gene05456 | MS.gene067755 | 0.822471 | 2.33E-53 | -1.69E-46 |
| MS.gene054618 | MS.gene067755 | 0.824567 | 7.53E-54 | -1.69E-46 |
| MS.gene055359 | MS.gene067755 | 0.801275 | 9.73E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene067755 | 0.810257 | 1.26E-50 | -1.69E-46 |
| MS.gene056629 | MS.gene067755 | 0.812953 | 3.27E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene067755 | 0.836887 | 7.19E-57 | -1.69E-46 |
| MS.gene059228 | MS.gene067755 | 0.860881 | 1.52E-63 | -1.69E-46 |
| MS.gene060005 | MS.gene067755 | 0.824467 | 7.94E-54 | -1.69E-46 |
| MS.gene060559 | MS.gene067755 | 0.800378 | 1.48E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067755.t1 | MTR_2g086680 | 67.442 | 172 | 54 | 2 | 1 | 170 | 1 | 172 | 5.62e-83 | 242 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067755.t1 | AT5G22430 | 47.134 | 157 | 81 | 2 | 15 | 170 | 17 | 172 | 1.50e-45 | 147 |
| MS.gene067755.t1 | AT2G27385 | 45.509 | 167 | 80 | 4 | 5 | 170 | 6 | 162 | 4.79e-41 | 135 |
| MS.gene067755.t1 | AT2G27385 | 45.181 | 166 | 82 | 3 | 5 | 170 | 17 | 173 | 6.61e-41 | 135 |
| MS.gene067755.t1 | AT2G27385 | 45.181 | 166 | 82 | 3 | 5 | 170 | 17 | 173 | 6.61e-41 | 135 |
Find 27 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCCTTACAATTTGTGTTT+TGG | 0.164797 | 4.2:-49528476 | None:intergenic |
| CAAGTTTCACAAGTTATTAA+AGG | 0.220047 | 4.2:+49528396 | MS.gene067755:CDS |
| AACTGTTTGGCAAAACTTCT+TGG | 0.296592 | 4.2:+49528339 | MS.gene067755:CDS |
| ATCAGCATGTGAAATTGTTC+AGG | 0.312927 | 4.2:+49527378 | MS.gene067755:CDS |
| CAAAGGAAGATCAATAGTTT+TGG | 0.317110 | 4.2:-49528517 | None:intergenic |
| TCTTCCTTTGCCTCCAGAAT+GGG | 0.330802 | 4.2:+49528530 | MS.gene067755:CDS |
| CTAGGTGCCAAACCCCATTC+TGG | 0.344968 | 4.2:-49528543 | None:intergenic |
| TGTAAGGCTGCAAAACCTAT+TGG | 0.353116 | 4.2:+49528489 | MS.gene067755:CDS |
| CTTTAATAACTTGTGAAACT+TGG | 0.355048 | 4.2:-49528395 | None:intergenic |
| AATTAAGGTATTCCAATGAT+AGG | 0.380107 | 4.2:-49528588 | None:intergenic |
| ACTCATAGCTACGATTTATC+TGG | 0.396323 | 4.2:+49527424 | MS.gene067755:CDS |
| TTTGCCTCCAGAATGGGGTT+TGG | 0.402516 | 4.2:+49528536 | MS.gene067755:CDS |
| ATCTTCCTTTGCCTCCAGAA+TGG | 0.404991 | 4.2:+49528529 | MS.gene067755:CDS |
| CTTCTGTAGTTGTCATAGCT+AGG | 0.461561 | 4.2:-49528263 | None:intergenic |
| GAACAATTTCACATGCTGAT+AGG | 0.462045 | 4.2:-49527375 | None:intergenic |
| TGAGGGAAGGTTAACCTTGA+AGG | 0.474424 | 4.2:-49528295 | None:intergenic |
| TGTTTGGCAAAACTTCTTGG+TGG | 0.491974 | 4.2:+49528342 | MS.gene067755:CDS |
| CTTCCTTTGCCTCCAGAATG+GGG | 0.503336 | 4.2:+49528531 | MS.gene067755:CDS |
| ATTCCAATGATAGGAACAAA+AGG | 0.504181 | 4.2:-49528579 | None:intergenic |
| AGAAGAAGATGGCTCCTTCA+AGG | 0.515472 | 4.2:+49528281 | MS.gene067755:CDS |
| CATGATATCAAGAAACTAAG+AGG | 0.542494 | 4.2:-49528450 | None:intergenic |
| AAACCCCATTCTGGAGGCAA+AGG | 0.556721 | 4.2:-49528534 | None:intergenic |
| TCAGCATGTGAAATTGTTCA+GGG | 0.561309 | 4.2:+49527379 | MS.gene067755:CDS |
| AAAGGTACATAATAGCTACT+AGG | 0.564274 | 4.2:-49528561 | None:intergenic |
| ATGACAACTACAGAAGAAGA+TGG | 0.604128 | 4.2:+49528270 | MS.gene067755:CDS |
| AAGGTCACAGTGAAATGTGA+AGG | 0.615631 | 4.2:+49528231 | MS.gene067755:CDS |
| GGTGCCAAACCCCATTCTGG+AGG | 0.664114 | 4.2:-49528540 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATAGTTTATTTATTAATA+GGG | + | chr4.2:49527615-49527634 | MS.gene067755:intron | 10.0% |
| !! | TCAATAGTTTATTTATTAAT+AGG | + | chr4.2:49527614-49527633 | MS.gene067755:intron | 10.0% |
| !!! | TAAAATTTTTCCCATAATTA+GGG | - | chr4.2:49527711-49527730 | None:intergenic | 15.0% |
| !! | AATAGTAGACATATGTATTA+TGG | + | chr4.2:49527531-49527550 | MS.gene067755:intron | 20.0% |
| !! | AATTAGGGACATTAAAAAAT+AGG | - | chr4.2:49527696-49527715 | None:intergenic | 20.0% |
| !! | ATCATGTATGTTTCTTTAAA+AGG | - | chr4.2:49527734-49527753 | None:intergenic | 20.0% |
| !! | GAACATTTAATTAAACTCAA+AGG | - | chr4.2:49527479-49527498 | None:intergenic | 20.0% |
| !! | TACAAGATGCATATAATATA+TGG | - | chr4.2:49528173-49528192 | None:intergenic | 20.0% |
| !! | TTTGTTAAGAAATACACATT+TGG | + | chr4.2:49527787-49527806 | MS.gene067755:intron | 20.0% |
| !!! | GTAAAATTTTTCCCATAATT+AGG | - | chr4.2:49527712-49527731 | None:intergenic | 20.0% |
| !!! | TAATTTTTTAGAAAGAGTGT+GGG | + | chr4.2:49528028-49528047 | MS.gene067755:intron | 20.0% |
| !!! | TCAATGATTTTCAGATATTA+AGG | + | chr4.2:49528212-49528231 | MS.gene067755:intron | 20.0% |
| !!! | TTTTTAATGTCCCTAATTAT+GGG | + | chr4.2:49527698-49527717 | MS.gene067755:intron | 20.0% |
| !!! | TTTTTTAATGTCCCTAATTA+TGG | + | chr4.2:49527697-49527716 | MS.gene067755:intron | 20.0% |
| ! | AAGGCCTTTACAAAAAATTA+CGG | - | chr4.2:49527880-49527899 | None:intergenic | 25.0% |
| ! | CAAGTTTCACAAGTTATTAA+AGG | + | chr4.2:49528396-49528415 | MS.gene067755:CDS | 25.0% |
| ! | TTAAACTCAAAGGCAAATTA+TGG | - | chr4.2:49527469-49527488 | None:intergenic | 25.0% |
| !! | CTTTAATAACTTGTGAAACT+TGG | - | chr4.2:49528398-49528417 | None:intergenic | 25.0% |
| !!! | ACAATAGTTTTTGTGTTGTT+TGG | - | chr4.2:49527767-49527786 | None:intergenic | 25.0% |
| !!! | GTAATTTTTTAGAAAGAGTG+TGG | + | chr4.2:49528027-49528046 | MS.gene067755:intron | 25.0% |
| !!! | TAAAAAATAGGAGCTTTGTT+TGG | - | chr4.2:49527684-49527703 | None:intergenic | 25.0% |
| AAAAACTTCCATGAACTGTT+TGG | + | chr4.2:49528326-49528345 | MS.gene067755:CDS | 30.0% | |
| AGGCCTTTACAAAAAATTAC+GGG | - | chr4.2:49527879-49527898 | None:intergenic | 30.0% | |
| ATTCCAATGATAGGAACAAA+AGG | - | chr4.2:49528582-49528601 | None:intergenic | 30.0% | |
| CATGATATCAAGAAACTAAG+AGG | - | chr4.2:49528453-49528472 | None:intergenic | 30.0% | |
| CTAAAAAATTACATGTGTCC+CGG | - | chr4.2:49528019-49528038 | None:intergenic | 30.0% | |
| TTGTTTGGAGATTAAAGCTA+GGG | - | chr4.2:49527669-49527688 | None:intergenic | 30.0% | |
| TTTATACCAGATGTCTCTTT+CGG | - | chr4.2:49527645-49527664 | None:intergenic | 30.0% | |
| TTTGTTTGGAGATTAAAGCT+AGG | - | chr4.2:49527670-49527689 | None:intergenic | 30.0% | |
| ! | AAAGGTACATAATAGCTACT+AGG | - | chr4.2:49528564-49528583 | None:intergenic | 30.0% |
| ! | AGCCTTACAATTTGTGTTTT+GGG | - | chr4.2:49528478-49528497 | None:intergenic | 30.0% |
| ! | CAAAGGAAGATCAATAGTTT+TGG | - | chr4.2:49528520-49528539 | None:intergenic | 30.0% |
| !! | TTTTTGGCATAAAGCTGAAT+TGG | - | chr4.2:49528369-49528388 | None:intergenic | 30.0% |
| !!! | ATAGTTTTGGAAGAACCAAT+AGG | - | chr4.2:49528507-49528526 | None:intergenic | 30.0% |
| !!! | GAAGTTTTTGTTTTGTCTGA+GGG | - | chr4.2:49528315-49528334 | None:intergenic | 30.0% |
| !!! | TGAAACTTGGTTTTGCTTTT+TGG | - | chr4.2:49528385-49528404 | None:intergenic | 30.0% |
| AACTGTTTGGCAAAACTTCT+TGG | + | chr4.2:49528339-49528358 | MS.gene067755:CDS | 35.0% | |
| ACTCATAGCTACGATTTATC+TGG | + | chr4.2:49527424-49527443 | MS.gene067755:CDS | 35.0% | |
| ATCAGCATGTGAAATTGTTC+AGG | + | chr4.2:49527378-49527397 | MS.gene067755:CDS | 35.0% | |
| ATGACAACTACAGAAGAAGA+TGG | + | chr4.2:49528270-49528289 | MS.gene067755:CDS | 35.0% | |
| CATCGACCATTTGATACATA+AGG | - | chr4.2:49528100-49528119 | None:intergenic | 35.0% | |
| CCTTTACAAAAAATTACGGG+AGG | - | chr4.2:49527876-49527895 | None:intergenic | 35.0% | |
| CTTAAGGTTCCAAGAATGTT+GGG | + | chr4.2:49528070-49528089 | MS.gene067755:intron | 35.0% | |
| GAACAATTTCACATGCTGAT+AGG | - | chr4.2:49527378-49527397 | None:intergenic | 35.0% | |
| GTATACTTCTAGACACATGT+TGG | - | chr4.2:49527949-49527968 | None:intergenic | 35.0% | |
| TACCTTGGAGATTTCACTTA+TGG | + | chr4.2:49527908-49527927 | MS.gene067755:intron | 35.0% | |
| TCAGCATGTGAAATTGTTCA+GGG | + | chr4.2:49527379-49527398 | MS.gene067755:CDS | 35.0% | |
| TTGAAGAACAATGAGAGTCA+TGG | - | chr4.2:49528196-49528215 | None:intergenic | 35.0% | |
| ! | AAGTTTTGCCAAACAGTTCA+TGG | - | chr4.2:49528337-49528356 | None:intergenic | 35.0% |
| ! | CAGCCTTACAATTTGTGTTT+TGG | - | chr4.2:49528479-49528498 | None:intergenic | 35.0% |
| ! | GCCTTACAATTTGTGTTTTG+GGG | - | chr4.2:49528477-49528496 | None:intergenic | 35.0% |
| ! | GTACCTTTTGTTCCTATCAT+TGG | + | chr4.2:49528576-49528595 | MS.gene067755:CDS | 35.0% |
| ! | TCTCCAAGGTATTATCAACA+AGG | - | chr4.2:49527899-49527918 | None:intergenic | 35.0% |
| !! | CCTTACAATTTGTGTTTTGG+GGG | - | chr4.2:49528476-49528495 | None:intergenic | 35.0% |
| !! | CTTTTTCACTCAGAAGAGTT+CGG | - | chr4.2:49527851-49527870 | None:intergenic | 35.0% |
| !!! | AAGCTGTGTTTTTTCCCATA+TGG | + | chr4.2:49527819-49527838 | MS.gene067755:intron | 35.0% |
| !!! | AGCTGTGTTTTTTCCCATAT+GGG | + | chr4.2:49527820-49527839 | MS.gene067755:intron | 35.0% |
| !!! | CCTCCCGTAATTTTTTGTAA+AGG | + | chr4.2:49527873-49527892 | MS.gene067755:intron | 35.0% |
| !!! | GGAAGTTTTTGTTTTGTCTG+AGG | - | chr4.2:49528316-49528335 | None:intergenic | 35.0% |
| !!! | TTTTTGTTTTGTCTGAGGGA+AGG | - | chr4.2:49528311-49528330 | None:intergenic | 35.0% |
| AAGCGTCTCAAAGTATACTC+CGG | + | chr4.2:49527997-49528016 | MS.gene067755:intron | 40.0% | |
| AAGGTCACAGTGAAATGTGA+AGG | + | chr4.2:49528231-49528250 | MS.gene067755:CDS | 40.0% | |
| ACTTCTAGACACATGTTGGT+TGG | - | chr4.2:49527945-49527964 | None:intergenic | 40.0% | |
| CCAACATTCTTGGAACCTTA+AGG | - | chr4.2:49528072-49528091 | None:intergenic | 40.0% | |
| CCCCCAAAACACAAATTGTA+AGG | + | chr4.2:49528473-49528492 | MS.gene067755:CDS | 40.0% | |
| CCTTAAGGTTCCAAGAATGT+TGG | + | chr4.2:49528069-49528088 | MS.gene067755:intron | 40.0% | |
| CTCCATAAGTGAAATCTCCA+AGG | - | chr4.2:49527913-49527932 | None:intergenic | 40.0% | |
| CTTCTGTAGTTGTCATAGCT+AGG | - | chr4.2:49528266-49528285 | None:intergenic | 40.0% | |
| GACCATTTGATACATAAGGC+CGG | - | chr4.2:49528096-49528115 | None:intergenic | 40.0% | |
| TGTAAGGCTGCAAAACCTAT+TGG | + | chr4.2:49528489-49528508 | MS.gene067755:CDS | 40.0% | |
| TGTTTGGCAAAACTTCTTGG+TGG | + | chr4.2:49528342-49528361 | MS.gene067755:CDS | 40.0% | |
| ! | CTTCTAGACACATGTTGGTT+GGG | - | chr4.2:49527944-49527963 | None:intergenic | 40.0% |
| !! | AGGCCTTGTTGATAATACCT+TGG | + | chr4.2:49527893-49527912 | MS.gene067755:intron | 40.0% |
| AAAGAAGCTCGCTTGCCTTA+AGG | + | chr4.2:49528054-49528073 | MS.gene067755:intron | 45.0% | |
| AGAAGAAGATGGCTCCTTCA+AGG | + | chr4.2:49528281-49528300 | MS.gene067755:CDS | 45.0% | |
| AGAGTTCGGATGTACCCATA+TGG | - | chr4.2:49527837-49527856 | None:intergenic | 45.0% | |
| AGCGTCTCAAAGTATACTCC+GGG | + | chr4.2:49527998-49528017 | MS.gene067755:intron | 45.0% | |
| ATCTTCCTTTGCCTCCAGAA+TGG | + | chr4.2:49528529-49528548 | MS.gene067755:CDS | 45.0% | |
| GAGTTCGGATGTACCCATAT+GGG | - | chr4.2:49527836-49527855 | None:intergenic | 45.0% | |
| TACCAGATGTCTCTTTCGGT+CGG | - | chr4.2:49527641-49527660 | None:intergenic | 45.0% | |
| TCTTCCTTTGCCTCCAGAAT+GGG | + | chr4.2:49528530-49528549 | MS.gene067755:CDS | 45.0% | |
| TGAGGGAAGGTTAACCTTGA+AGG | - | chr4.2:49528298-49528317 | None:intergenic | 45.0% | |
| AGGTTCCAAGAATGTTGGGC+CGG | + | chr4.2:49528074-49528093 | MS.gene067755:intron | 50.0% | |
| CTTCCTTTGCCTCCAGAATG+GGG | + | chr4.2:49528531-49528550 | MS.gene067755:CDS | 50.0% | |
| TTTGCCTCCAGAATGGGGTT+TGG | + | chr4.2:49528536-49528555 | MS.gene067755:CDS | 50.0% | |
| ! | AAACCCCATTCTGGAGGCAA+AGG | - | chr4.2:49528537-49528556 | None:intergenic | 50.0% |
| ! | GGCCGGCCTTATGTATCAAA+TGG | + | chr4.2:49528091-49528110 | MS.gene067755:intron | 50.0% |
| CTAGGTGCCAAACCCCATTC+TGG | - | chr4.2:49528546-49528565 | None:intergenic | 55.0% | |
| TAAGGCCGGCCCAACATTCT+TGG | - | chr4.2:49528082-49528101 | None:intergenic | 55.0% | |
| GGCCGACCGAAAGAGACATC+TGG | + | chr4.2:49527636-49527655 | MS.gene067755:intron | 60.0% | |
| GGTGCCAAACCCCATTCTGG+AGG | - | chr4.2:49528543-49528562 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 49527316 | 49528608 | 49527316 | ID=MS.gene067755 |
| chr4.2 | mRNA | 49527316 | 49528608 | 49527316 | ID=MS.gene067755.t1;Parent=MS.gene067755 |
| chr4.2 | exon | 49527316 | 49527445 | 49527316 | ID=MS.gene067755.t1.exon1;Parent=MS.gene067755.t1 |
| chr4.2 | CDS | 49527316 | 49527445 | 49527316 | ID=cds.MS.gene067755.t1;Parent=MS.gene067755.t1 |
| chr4.2 | exon | 49528226 | 49528608 | 49528226 | ID=MS.gene067755.t1.exon2;Parent=MS.gene067755.t1 |
| chr4.2 | CDS | 49528226 | 49528608 | 49528226 | ID=cds.MS.gene067755.t1;Parent=MS.gene067755.t1 |
| Gene Sequence |
| Protein sequence |
