Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067857.t1 | AAC25776.1 | 100 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 3.20E-14 | 88.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067857.t1 | Q40334 | 100.0 | 105 | 0 | 0 | 1 | 105 | 1 | 105 | 2.3e-14 | 88.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049438 | MS.gene067857 | 0.801776 | 7.68E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene067857 | 0.803594 | 3.24E-49 | -1.69E-46 |
| MS.gene051856 | MS.gene067857 | 0.801025 | 1.09E-48 | -1.69E-46 |
| MS.gene053295 | MS.gene067857 | 0.816091 | 6.61E-52 | -1.69E-46 |
| MS.gene05344 | MS.gene067857 | -0.812209 | 4.76E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene067857 | 0.815249 | 1.02E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene067857 | 0.801739 | 7.81E-49 | -1.69E-46 |
| MS.gene057055 | MS.gene067857 | 0.805632 | 1.22E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene067857 | 0.802546 | 5.33E-49 | -1.69E-46 |
| MS.gene05781 | MS.gene067857 | 0.808648 | 2.79E-50 | -1.69E-46 |
| MS.gene058054 | MS.gene067857 | 0.814453 | 1.53E-51 | -1.69E-46 |
| MS.gene058646 | MS.gene067857 | 0.803549 | 3.31E-49 | -1.69E-46 |
| MS.gene058654 | MS.gene067857 | 0.811312 | 7.46E-51 | -1.69E-46 |
| MS.gene059050 | MS.gene067857 | 0.805773 | 1.14E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene067857 | 0.804529 | 2.07E-49 | -1.69E-46 |
| MS.gene060869 | MS.gene067857 | 0.824965 | 6.06E-54 | -1.69E-46 |
| MS.gene060870 | MS.gene067857 | 0.82658 | 2.51E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067857.t1 | MTR_7g086340 | 83.065 | 124 | 6 | 1 | 1 | 124 | 1 | 109 | 4.56e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATGTTGTTCTCCTTTG+TGG | 0.347404 | 7.1:-19068443 | None:intergenic |
| AAAGGAGAGCAACATGGATT+TGG | 0.348363 | 7.1:+19068492 | MS.gene067857:CDS |
| GGAGAACAACATGGATTTGT+TGG | 0.387551 | 7.1:+19068405 | MS.gene067857:CDS |
| TGTCACTGCTGCTGCTATCA+TGG | 0.437381 | 7.1:-19068665 | None:intergenic |
| AAGATCAAGGACAAAATTCA+TGG | 0.463252 | 7.1:+19068573 | MS.gene067857:CDS |
| CTTGTCTCCTCCTCCGTGAA+GGG | 0.466823 | 7.1:-19068316 | None:intergenic |
| TCTTGTCTCCTCCTCCGTGA+AGG | 0.469459 | 7.1:-19068317 | None:intergenic |
| GCAGGAATCATGAACAAGAT+TGG | 0.500782 | 7.1:+19068288 | MS.gene067857:CDS |
| AAGAAGGAGAAGAAGAAACA+TGG | 0.508687 | 7.1:+19068624 | MS.gene067857:CDS |
| CCTCCTTGTGCTCTTCCCCA+TGG | 0.520638 | 7.1:-19068539 | None:intergenic |
| AAGATTGGTGATGCCCTTCA+CGG | 0.523694 | 7.1:+19068303 | MS.gene067857:CDS |
| GGTGATGCCCTTCACGGAGG+AGG | 0.538006 | 7.1:+19068309 | MS.gene067857:CDS |
| GGATTTGTTGGAGGACATGC+TGG | 0.539276 | 7.1:+19068417 | MS.gene067857:CDS |
| ATACCATGGGGAAGAGCACA+AGG | 0.548711 | 7.1:+19068536 | MS.gene067857:CDS |
| CGGAGGAGGAGACAAGAAAG+AGG | 0.550744 | 7.1:+19068323 | MS.gene067857:CDS |
| GGGATTTGCCGACAAGATCA+AGG | 0.551089 | 7.1:+19068560 | MS.gene067857:CDS |
| AAATAGAAATACAATATGGC+AGG | 0.556347 | 7.1:+19068270 | None:intergenic |
| GGAGAACAACATGGGCATGT+AGG | 0.561384 | 7.1:+19068357 | MS.gene067857:CDS |
| CATGGAGAGGGTCATGAACA+TGG | 0.563434 | 7.1:+19068642 | MS.gene067857:CDS |
| GACCACAAGGAGGGATACCA+TGG | 0.564363 | 7.1:+19068522 | MS.gene067857:CDS |
| GAACAACATGGATTTGTTGG+AGG | 0.585288 | 7.1:+19068408 | MS.gene067857:CDS |
| GAGCACAAAGGAGAACAACA+TGG | 0.591423 | 7.1:+19068348 | MS.gene067857:CDS |
| AAGGACAAAATTCATGGTGA+AGG | 0.593365 | 7.1:+19068579 | MS.gene067857:CDS |
| GAGCAACATGGATTTGGTCA+TGG | 0.593720 | 7.1:+19068498 | MS.gene067857:CDS |
| GAACAACATGGGCATGTAGG+AGG | 0.600098 | 7.1:+19068360 | MS.gene067857:CDS |
| GAGAAGAAGAAACATGGAGA+GGG | 0.604104 | 7.1:+19068630 | MS.gene067857:CDS |
| AAGAAAGAGGGAGAGCACAA+AGG | 0.613945 | 7.1:+19068336 | MS.gene067857:CDS |
| ATTCATGGTGAAGGTGCAGA+TGG | 0.614174 | 7.1:+19068588 | MS.gene067857:CDS |
| CATGTAGGAGGAGAACATCA+TGG | 0.615237 | 7.1:+19068372 | MS.gene067857:CDS |
| CCCCATGGTATCCCTCCTTG+TGG | 0.622941 | 7.1:-19068524 | None:intergenic |
| GGAGGAGGAGACAAGAAAGA+GGG | 0.626482 | 7.1:+19068324 | MS.gene067857:CDS |
| ATTTGGTCATGGAGACCACA+AGG | 0.646260 | 7.1:+19068509 | MS.gene067857:CDS |
| GATTACAAAGGAGAGCAACA+TGG | 0.647436 | 7.1:+19068486 | MS.gene067857:CDS |
| GGTCATGGAGACCACAAGGA+GGG | 0.647519 | 7.1:+19068513 | MS.gene067857:CDS |
| ACCACAAGGAGGGATACCAT+GGG | 0.658332 | 7.1:+19068523 | MS.gene067857:CDS |
| CATGGGGAAGAGCACAAGGA+GGG | 0.663399 | 7.1:+19068540 | MS.gene067857:CDS |
| AGCACAAAGGAGAACAACAT+GGG | 0.665854 | 7.1:+19068349 | MS.gene067857:CDS |
| GAGTACAAAGGAGAACAACA+TGG | 0.667533 | 7.1:+19068396 | MS.gene067857:CDS |
| GGAGAAGAAGAAACATGGAG+AGG | 0.668303 | 7.1:+19068629 | MS.gene067857:CDS |
| GAACATCATGGTGAGTACAA+AGG | 0.675462 | 7.1:+19068384 | MS.gene067857:CDS |
| GGACATGCTGGTGACCACAA+AGG | 0.680130 | 7.1:+19068429 | MS.gene067857:CDS |
| GACCACAAAGGAGAACAACA+TGG | 0.705961 | 7.1:+19068441 | MS.gene067857:CDS |
| TGGTCATGGAGACCACAAGG+AGG | 0.712485 | 7.1:+19068512 | MS.gene067857:CDS |
| CCATGGGGAAGAGCACAAGG+AGG | 0.727994 | 7.1:+19068539 | MS.gene067857:CDS |
| ATTGGTGATGCCCTTCACGG+AGG | 0.747767 | 7.1:+19068306 | MS.gene067857:CDS |
| CCACAAGGAGGGATACCATG+GGG | 0.757421 | 7.1:+19068524 | MS.gene067857:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGATCAAGGACAAAATTCA+TGG | + | chr7.1:19068573-19068592 | MS.gene067857:CDS | 30.0% | |
| TGGTGAAAAGAAAAAGAAGA+AGG | + | chr7.1:19068608-19068627 | MS.gene067857:CDS | 30.0% | |
| ! | AATTTTGTCCTTGATCTTGT+CGG | - | chr7.1:19068571-19068590 | None:intergenic | 30.0% |
| AAGAAGGAGAAGAAGAAACA+TGG | + | chr7.1:19068624-19068643 | MS.gene067857:CDS | 35.0% | |
| AAGGACAAAATTCATGGTGA+AGG | + | chr7.1:19068579-19068598 | MS.gene067857:CDS | 35.0% | |
| AAAGGAGAGCAACATGGATT+TGG | + | chr7.1:19068492-19068511 | MS.gene067857:CDS | 40.0% | |
| AACCATGTTGTTCTCCTTTG+TGG | - | chr7.1:19068446-19068465 | None:intergenic | 40.0% | |
| AGCACAAAGGAGAACAACAT+GGG | + | chr7.1:19068349-19068368 | MS.gene067857:CDS | 40.0% | |
| GAACATCATGGTGAGTACAA+AGG | + | chr7.1:19068384-19068403 | MS.gene067857:CDS | 40.0% | |
| GAGAAGAAGAAACATGGAGA+GGG | + | chr7.1:19068630-19068649 | MS.gene067857:CDS | 40.0% | |
| GAGTACAAAGGAGAACAACA+TGG | + | chr7.1:19068396-19068415 | MS.gene067857:CDS | 40.0% | |
| GATTACAAAGGAGAGCAACA+TGG | + | chr7.1:19068486-19068505 | MS.gene067857:CDS | 40.0% | |
| GCAGGAATCATGAACAAGAT+TGG | + | chr7.1:19068288-19068307 | MS.gene067857:CDS | 40.0% | |
| GGAGAACAACATGGATTTGT+TGG | + | chr7.1:19068405-19068424 | MS.gene067857:CDS | 40.0% | |
| !! | GAACAACATGGATTTGTTGG+AGG | + | chr7.1:19068408-19068427 | MS.gene067857:CDS | 40.0% |
| !!! | ATGGTTTTGTAGGAGGACAT+GGG | + | chr7.1:19068460-19068479 | MS.gene067857:CDS | 40.0% |
| !!! | GAACAACATGGTTTTGTAGG+AGG | + | chr7.1:19068453-19068472 | MS.gene067857:CDS | 40.0% |
| !!! | GGAGAACAACATGGTTTTGT+AGG | + | chr7.1:19068450-19068469 | MS.gene067857:CDS | 40.0% |
| ATTTGGTCATGGAGACCACA+AGG | + | chr7.1:19068509-19068528 | MS.gene067857:CDS | 45.0% | |
| CATGTAGGAGGAGAACATCA+TGG | + | chr7.1:19068372-19068391 | MS.gene067857:CDS | 45.0% | |
| GACCACAAAGGAGAACAACA+TGG | + | chr7.1:19068441-19068460 | MS.gene067857:CDS | 45.0% | |
| GAGCACAAAGGAGAACAACA+TGG | + | chr7.1:19068348-19068367 | MS.gene067857:CDS | 45.0% | |
| GGAGAAGAAGAAACATGGAG+AGG | + | chr7.1:19068629-19068648 | MS.gene067857:CDS | 45.0% | |
| ! | AAGAAAGAGGGAGAGCACAA+AGG | + | chr7.1:19068336-19068355 | MS.gene067857:CDS | 45.0% |
| ! | ATTCATGGTGAAGGTGCAGA+TGG | + | chr7.1:19068588-19068607 | MS.gene067857:CDS | 45.0% |
| ! | GAGCAACATGGATTTGGTCA+TGG | + | chr7.1:19068498-19068517 | MS.gene067857:CDS | 45.0% |
| !! | AAGATTGGTGATGCCCTTCA+CGG | + | chr7.1:19068303-19068322 | MS.gene067857:CDS | 45.0% |
| !!! | CATGGTTTTGTAGGAGGACA+TGG | + | chr7.1:19068459-19068478 | MS.gene067857:CDS | 45.0% |
| !!! | TGGTTTTGTAGGAGGACATG+GGG | + | chr7.1:19068461-19068480 | MS.gene067857:CDS | 45.0% |
| ACCACAAGGAGGGATACCAT+GGG | + | chr7.1:19068523-19068542 | MS.gene067857:CDS | 50.0% | |
| CATGGAGAGGGTCATGAACA+TGG | + | chr7.1:19068642-19068661 | MS.gene067857:CDS | 50.0% | |
| GAACAACATGGGCATGTAGG+AGG | + | chr7.1:19068360-19068379 | MS.gene067857:CDS | 50.0% | |
| GGACATGGGGGTGATTACAA+AGG | + | chr7.1:19068474-19068493 | MS.gene067857:CDS | 50.0% | |
| GGAGAACAACATGGGCATGT+AGG | + | chr7.1:19068357-19068376 | MS.gene067857:CDS | 50.0% | |
| GGAGGAGGAGACAAGAAAGA+GGG | + | chr7.1:19068324-19068343 | MS.gene067857:CDS | 50.0% | |
| GGGATTTGCCGACAAGATCA+AGG | + | chr7.1:19068560-19068579 | MS.gene067857:CDS | 50.0% | |
| TGTCACTGCTGCTGCTATCA+TGG | - | chr7.1:19068668-19068687 | None:intergenic | 50.0% | |
| ! | ATACCATGGGGAAGAGCACA+AGG | + | chr7.1:19068536-19068555 | MS.gene067857:CDS | 50.0% |
| !! | GGATTTGTTGGAGGACATGC+TGG | + | chr7.1:19068417-19068436 | MS.gene067857:CDS | 50.0% |
| !! | GGTTTTGTAGGAGGACATGG+GGG | + | chr7.1:19068462-19068481 | MS.gene067857:CDS | 50.0% |
| CCACAAGGAGGGATACCATG+GGG | + | chr7.1:19068524-19068543 | MS.gene067857:CDS | 55.0% | |
| CGGAGGAGGAGACAAGAAAG+AGG | + | chr7.1:19068323-19068342 | MS.gene067857:CDS | 55.0% | |
| CTTGTCTCCTCCTCCGTGAA+GGG | - | chr7.1:19068319-19068338 | None:intergenic | 55.0% | |
| GACCACAAGGAGGGATACCA+TGG | + | chr7.1:19068522-19068541 | MS.gene067857:CDS | 55.0% | |
| GGACATGCTGGTGACCACAA+AGG | + | chr7.1:19068429-19068448 | MS.gene067857:CDS | 55.0% | |
| GGTCATGGAGACCACAAGGA+GGG | + | chr7.1:19068513-19068532 | MS.gene067857:CDS | 55.0% | |
| TCTTGTCTCCTCCTCCGTGA+AGG | - | chr7.1:19068320-19068339 | None:intergenic | 55.0% | |
| TGGTCATGGAGACCACAAGG+AGG | + | chr7.1:19068512-19068531 | MS.gene067857:CDS | 55.0% | |
| ! | CATGGGGAAGAGCACAAGGA+GGG | + | chr7.1:19068540-19068559 | MS.gene067857:CDS | 55.0% |
| !! | ATTGGTGATGCCCTTCACGG+AGG | + | chr7.1:19068306-19068325 | MS.gene067857:CDS | 55.0% |
| CCCCATGGTATCCCTCCTTG+TGG | - | chr7.1:19068527-19068546 | None:intergenic | 60.0% | |
| CCTCCTTGTGCTCTTCCCCA+TGG | - | chr7.1:19068542-19068561 | None:intergenic | 60.0% | |
| ! | CCATGGGGAAGAGCACAAGG+AGG | + | chr7.1:19068539-19068558 | MS.gene067857:CDS | 60.0% |
| GGTGATGCCCTTCACGGAGG+AGG | + | chr7.1:19068309-19068328 | MS.gene067857:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 19068285 | 19068695 | 19068285 | ID=MS.gene067857 |
| chr7.1 | mRNA | 19068285 | 19068695 | 19068285 | ID=MS.gene067857.t1;Parent=MS.gene067857 |
| chr7.1 | exon | 19068285 | 19068695 | 19068285 | ID=MS.gene067857.t1.exon1;Parent=MS.gene067857.t1 |
| chr7.1 | CDS | 19068285 | 19068695 | 19068285 | ID=cds.MS.gene067857.t1;Parent=MS.gene067857.t1 |
| Gene Sequence |
| Protein sequence |
