AlfalfaGEDB | MS.gene068237

M. sativa cultivar XinJiangDaYe / MS.gene068237

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068237.t1	RHN76190.1	88.1	109	13	0	6	114	788	896	1.90E-44	188.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068237.t1	A0A072VBT1	88.1	109	13	0	6	114	581	689	1.4e-44	188.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene068237	0.844126	9.17E-59	-1.69E-46
MS.gene049404	MS.gene068237	0.800068	1.71E-48	-1.69E-46
MS.gene049435	MS.gene068237	0.809161	2.17E-50	-1.69E-46
MS.gene049501	MS.gene068237	0.808313	3.29E-50	-1.69E-46
MS.gene050442	MS.gene068237	0.839761	1.31E-57	-1.69E-46
MS.gene050719	MS.gene068237	0.80383	2.89E-49	-1.69E-46
MS.gene051247	MS.gene068237	0.804761	1.85E-49	-1.69E-46
MS.gene051265	MS.gene068237	0.805577	1.25E-49	-1.69E-46
MS.gene052020	MS.gene068237	0.841676	4.11E-58	-1.69E-46
MS.gene052323	MS.gene068237	0.800595	1.34E-48	-1.69E-46
MS.gene052611	MS.gene068237	0.80485	1.77E-49	-1.69E-46
MS.gene052896	MS.gene068237	0.803254	3.81E-49	-1.69E-46
MS.gene054467	MS.gene068237	0.813581	2.38E-51	-1.69E-46
MS.gene055357	MS.gene068237	0.813229	2.84E-51	-1.69E-46
MS.gene055818	MS.gene068237	0.808672	2.76E-50	-1.69E-46
MS.gene056173	MS.gene068237	0.830934	2.22E-55	-1.69E-46
MS.gene057869	MS.gene068237	0.817695	2.89E-52	-1.69E-46
MS.gene058010	MS.gene068237	0.801543	8.57E-49	-1.69E-46
MS.gene058168	MS.gene068237	0.801394	9.19E-49	-1.69E-46
MS.gene059570	MS.gene068237	0.802033	6.80E-49	-1.69E-46
MS.gene060137	MS.gene068237	-0.824058	9.91E-54	-1.69E-46
MS.gene060803	MS.gene068237	0.802197	6.29E-49	-1.69E-46
MS.gene060998	MS.gene068237	0.805153	1.53E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068237.t1	MTR_2g098830	88.073	109	13	0	6	114	581	689	2.00e-59	196
MS.gene068237.t1	MTR_2g098830	67.213	61	20	0	6	66	1071	1131	2.36e-20	85.1
MS.gene068237.t1	MTR_2g098830	88.073	109	13	0	6	114	848	956	2.27e-59	196
MS.gene068237.t1	MTR_2g098830	67.213	61	20	0	6	66	1338	1398	2.29e-20	85.1
MS.gene068237.t1	MTR_2g098830	76.087	46	10	1	69	114	1	45	6.04e-16	72.8
MS.gene068237.t1	MTR_4g067090	67.442	43	14	0	72	114	5	47	6.16e-14	66.6
MS.gene068237.t1	MTR_0002s1280	60.870	46	18	0	69	114	1	46	1.25e-12	62.8
MS.gene068237.t1	MTR_2g098770	60.870	46	18	0	69	114	1	46	2.30e-12	62.4
MS.gene068237.t1	MTR_2g098720	58.696	46	19	0	69	114	1	46	4.43e-12	61.2
MS.gene068237.t1	MTR_2g022300	54.348	46	21	0	69	114	1	46	1.03e-11	57.0
MS.gene068237.t1	MTR_2g098730	58.696	46	19	0	69	114	1	46	1.29e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 27 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTTCTGCTGTTGAGATAT+TGG	0.266453	2.3:+4626173	None:intergenic
TGTTGAAATTATCTGCAATC+AGG	0.361667	2.3:+4626345	None:intergenic
ACAGCAGAAAGTTGCAAAAC+AGG	0.369097	2.3:-4626162	None:intergenic
GTCCTACTGTGGATGCAGTT+AGG	0.395558	2.3:-4626259	MS.gene068237:CDS
GAAGCCTTTGCTGGCAGCAT+TGG	0.419671	2.3:+4626212	None:intergenic
TCCTACTGTGGATGCAGTTA+GGG	0.428934	2.3:-4626258	MS.gene068237:CDS
CTACATGCCATTGGATTGGC+AGG	0.452749	2.3:-4626486	None:intergenic
CATAGCTTCGAAGAGGAAGA+TGG	0.455683	2.3:-4626297	MS.gene068237:CDS
TCACGGCTAGAAGCCTTTGC+TGG	0.462181	2.3:+4626203	None:intergenic
TAGGGCATTCGTTGAACACT+TGG	0.464155	2.3:-4626240	MS.gene068237:CDS
CCCATCTAACTACATGCCAT+TGG	0.502094	2.3:-4626495	None:intergenic
TTCTGCTGTTGAGATATTGG+AGG	0.535331	2.3:+4626176	None:intergenic
ATTGGATTGGCAGGCTGAGA+AGG	0.536680	2.3:-4626477	MS.gene068237:CDS
CTAACTACATGCCATTGGAT+TGG	0.537594	2.3:-4626490	None:intergenic
TGATCCAATGCTGCCAGCAA+AGG	0.549274	2.3:-4626216	MS.gene068237:CDS
GTTGAAATTATCTGCAATCA+GGG	0.555509	2.3:+4626346	None:intergenic
TTCTCAGCCTGCCAATCCAA+TGG	0.556666	2.3:+4626479	None:intergenic
CTGAGAAGGTTGTTTCAACA+AGG	0.573360	2.3:-4626463	MS.gene068237:CDS
ATCTGATGTGTGTCCTACTG+TGG	0.606281	2.3:-4626270	MS.gene068237:CDS
AGGATGCACATTACTGAGGG+TGG	0.608899	2.3:-4626443	MS.gene068237:CDS
ATAGCTTCGAAGAGGAAGAT+GGG	0.609022	2.3:-4626296	MS.gene068237:CDS
AACAAGGATGCACATTACTG+AGG	0.610296	2.3:-4626447	MS.gene068237:CDS
ACAAGGATGCACATTACTGA+GGG	0.616230	2.3:-4626446	MS.gene068237:CDS
AAAGTTTCATAGCTTCGAAG+AGG	0.617952	2.3:-4626304	MS.gene068237:CDS
GAGATATTGGAGGTGCATCA+CGG	0.628198	2.3:+4626186	None:intergenic
TAAGATTTGTGCTGATGCCA+CGG	0.632079	2.3:-4626396	MS.gene068237:CDS
GCCCTAACTGCATCCACAGT+AGG	0.676357	2.3:+4626257	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTGAAATTATCTGCAATCA+GGG	+	chr2.3:4626302-4626321	None:intergenic	30.0%
	TGTTGAAATTATCTGCAATC+AGG	+	chr2.3:4626303-4626322	None:intergenic	30.0%
!	AATCAGGGTACATTTTTCTT+TGG	+	chr2.3:4626287-4626306	None:intergenic	30.0%
	AAAGTTTCATAGCTTCGAAG+AGG	-	chr2.3:4626341-4626360	MS.gene068237:CDS	35.0%
	ACTTTCTGCTGTTGAGATAT+TGG	+	chr2.3:4626475-4626494	None:intergenic	35.0%
	AACAAGGATGCACATTACTG+AGG	-	chr2.3:4626198-4626217	MS.gene068237:CDS	40.0%
	ACAAGGATGCACATTACTGA+GGG	-	chr2.3:4626199-4626218	MS.gene068237:CDS	40.0%
	ATAGCTTCGAAGAGGAAGAT+GGG	-	chr2.3:4626349-4626368	MS.gene068237:CDS	40.0%
	TTCTGCTGTTGAGATATTGG+AGG	+	chr2.3:4626472-4626491	None:intergenic	40.0%
!	CTGAGAAGGTTGTTTCAACA+AGG	-	chr2.3:4626182-4626201	MS.gene068237:CDS	40.0%
!	TAAGATTTGTGCTGATGCCA+CGG	-	chr2.3:4626249-4626268	MS.gene068237:CDS	40.0%
!	TTTGGATTCGTTTTCAACCG+TGG	+	chr2.3:4626269-4626288	None:intergenic	40.0%
	ATCTGATGTGTGTCCTACTG+TGG	-	chr2.3:4626375-4626394	MS.gene068237:CDS	45.0%
	CATAGCTTCGAAGAGGAAGA+TGG	-	chr2.3:4626348-4626367	MS.gene068237:CDS	45.0%
	TCCTACTGTGGATGCAGTTA+GGG	-	chr2.3:4626387-4626406	MS.gene068237:CDS	45.0%
!	GAGATATTGGAGGTGCATCA+CGG	+	chr2.3:4626462-4626481	None:intergenic	45.0%
!	TAGGGCATTCGTTGAACACT+TGG	-	chr2.3:4626405-4626424	MS.gene068237:CDS	45.0%
	AGGATGCACATTACTGAGGG+TGG	-	chr2.3:4626202-4626221	MS.gene068237:CDS	50.0%
	GTCCTACTGTGGATGCAGTT+AGG	-	chr2.3:4626386-4626405	MS.gene068237:CDS	50.0%
	TGATCCAATGCTGCCAGCAA+AGG	-	chr2.3:4626429-4626448	MS.gene068237:CDS	50.0%
	TTCTCAGCCTGCCAATCCAA+TGG	+	chr2.3:4626169-4626188	None:intergenic	50.0%
!!	ATTGGATTGGCAGGCTGAGA+AGG	-	chr2.3:4626168-4626187	MS.gene068237:CDS	50.0%
	GAAGCCTTTGCTGGCAGCAT+TGG	+	chr2.3:4626436-4626455	None:intergenic	55.0%
	GCCCTAACTGCATCCACAGT+AGG	+	chr2.3:4626391-4626410	None:intergenic	55.0%
	TCACGGCTAGAAGCCTTTGC+TGG	+	chr2.3:4626445-4626464	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	4626163	4626504	4626163	ID=MS.gene068237
chr2.3	mRNA	4626163	4626504	4626163	ID=MS.gene068237.t1;Parent=MS.gene068237
chr2.3	exon	4626163	4626504	4626163	ID=MS.gene068237.t1.exon1;Parent=MS.gene068237.t1
chr2.3	CDS	4626163	4626504	4626163	ID=cds.MS.gene068237.t1;Parent=MS.gene068237.t1

Gene Sequence	>MS.gene068237 ATGCCATTGGATTGGCAGGCTGAGAAGGTTGTTTCAACAAGGATGCACATTACTGAGGGTGGAATCAAAGATGAATCTGCTTTTGATAAGATTTGTGCTGATGCCACGGTTGAAAACGAATCCAAAGAAAAATGTACCCTGATTGCAGATAATTTCAACACAGATTTTGAGAAAGTTCAAAGTTTCATAGCTTCGAAGAGGAAGATGGGTCGATCTGATGTGTGTCCTACTGTGGATGCAGTTAGGGCATTCGTTGAACACTTGGTTGATCCAATGCTGCCAGCAAAGGCTTCTAGCCGTGATGCACCTCCAATATCTCAACAGCAGAAAGTTGCAAAACAG
Protein sequence	>MS.gene068237.t1 MPLDWQAEKVVSTRMHITEGGIKDESAFDKICADATVENESKEKCTLIADNFNTDFEKVQSFIASKRKMGRSDVCPTVDAVRAFVEHLVDPMLPAKASSRDAPPISQQQKVAKQ