AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068475

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068475.t1 MTR_6g065340 86.610 351 31 1 1 351 1 335 0.0 604
MS.gene068475.t1 MTR_6g065270 83.976 337 50 1 19 351 6 342 0.0 597
MS.gene068475.t1 MTR_6g065370 80.236 339 67 0 13 351 2 340 0.0 573
MS.gene068475.t1 MTR_6g065400 79.646 339 69 0 13 351 2 340 0.0 571
MS.gene068475.t1 MTR_6g065120 78.299 341 73 1 12 351 2 342 0.0 560
MS.gene068475.t1 MTR_6g065110 68.485 330 104 0 16 345 6 335 3.71e-177 495
MS.gene068475.t1 MTR_6g065430 59.819 331 124 2 18 346 6 329 2.73e-142 406
MS.gene068475.t1 MTR_1g082210 38.109 349 206 4 8 350 1 345 2.56e-77 241
MS.gene068475.t1 MTR_1g089310 38.177 351 206 5 1 345 12 357 3.90e-77 241
MS.gene068475.t1 MTR_1g089310 38.728 346 199 6 8 345 1 341 1.44e-76 239
MS.gene068475.t1 MTR_7g093950 36.494 348 210 5 8 345 1 347 3.60e-74 233
MS.gene068475.t1 MTR_6g464280 36.271 295 176 7 58 345 36 325 2.90e-51 173
MS.gene068475.t1 MTR_7g406880 33.003 303 176 8 66 346 54 351 6.75e-46 160
MS.gene068475.t1 MTR_6g464510 33.441 311 190 7 42 345 27 327 9.83e-46 159
MS.gene068475.t1 MTR_8g035540 33.439 314 188 6 42 344 13 316 1.06e-45 159
MS.gene068475.t1 MTR_8g035520 32.344 337 202 8 22 347 6 327 2.30e-45 158
MS.gene068475.t1 MTR_4g050140 32.281 285 175 4 71 346 42 317 4.23e-44 154
MS.gene068475.t1 MTR_1g102160 33.228 316 185 9 42 344 23 325 3.94e-42 149
MS.gene068475.t1 MTR_0007s0520 34.201 269 157 6 59 317 39 297 8.14e-42 149
MS.gene068475.t1 MTR_6g464320 34.981 263 158 5 58 312 37 294 1.09e-41 148
MS.gene068475.t1 MTR_1g102170 38.164 207 111 5 70 270 69 264 2.50e-41 147
MS.gene068475.t1 MTR_8g035500 31.731 312 192 7 43 344 17 317 3.34e-41 147
MS.gene068475.t1 MTR_7g107040 31.058 293 181 5 66 348 39 320 5.61e-40 144
MS.gene068475.t1 MTR_1g012430 31.290 310 193 9 42 346 15 309 2.40e-39 142
MS.gene068475.t1 MTR_3g012440 31.392 309 198 3 52 346 34 342 4.80e-39 142
MS.gene068475.t1 MTR_1g111280 32.013 303 173 9 59 344 52 338 5.82e-39 142
MS.gene068475.t1 MTR_6g464120 36.493 211 124 4 60 267 43 246 1.87e-37 141
MS.gene068475.t1 MTR_4g086540 30.492 305 178 8 49 348 33 308 5.13e-37 135
MS.gene068475.t1 MTR_4g086510 31.186 295 183 7 60 345 35 318 9.71e-36 132
MS.gene068475.t1 MTR_3g101420 30.000 300 180 7 60 346 34 316 1.02e-35 132
MS.gene068475.t1 MTR_4g086480 30.952 294 180 6 60 344 39 318 3.82e-34 128
MS.gene068475.t1 MTR_7g056207 31.000 300 186 7 60 350 67 354 6.20e-34 128
MS.gene068475.t1 MTR_4g086470 30.743 296 182 6 60 346 38 319 2.24e-29 115
MS.gene068475.t1 MTR_6g022710 28.727 275 150 5 102 335 156 425 1.77e-27 112
MS.gene068475.t1 MTR_1g105020 27.797 295 187 7 64 344 40 322 3.04e-27 109
MS.gene068475.t1 MTR_3g450680 27.241 290 185 7 64 339 48 325 8.95e-26 105
MS.gene068475.t1 MTR_6g065470 36.842 171 76 3 18 170 6 162 7.54e-25 99.4
MS.gene068475.t1 MTR_5g005850 26.959 319 200 10 43 344 26 328 8.88e-25 103
MS.gene068475.t1 MTR_8g040860 25.333 300 194 8 57 339 36 322 9.54e-23 97.4
MS.gene068475.t1 MTR_5g005900 26.531 294 191 7 68 346 45 328 1.89e-22 96.7
MS.gene068475.t1 MTR_7g051020 29.373 303 186 8 59 346 38 327 4.39e-21 92.8
MS.gene068475.t1 MTR_5g005920 25.076 331 217 11 33 346 16 332 8.34e-21 92.0
MS.gene068475.t1 MTR_1g104930 29.310 290 179 7 70 346 48 324 1.12e-18 85.9
MS.gene068475.t1 MTR_1g104933 28.276 290 178 8 70 346 46 318 4.05e-16 78.6
MS.gene068475.t1 MTR_6g065480 62.745 51 18 1 244 294 1 50 1.15e-14 68.2
MS.gene068475.t1 MTR_0007s0480 28.977 176 110 6 182 346 1 172 1.39e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068475.t1 AT5G23530 54.360 344 139 4 11 350 3 332 7.26e-136 390
MS.gene068475.t1 AT5G27320 38.192 343 204 3 8 345 1 340 3.20e-79 246
MS.gene068475.t1 AT3G63010 36.919 344 205 5 15 351 10 348 3.04e-72 229
MS.gene068475.t1 AT3G05120 35.362 345 213 3 8 345 1 342 2.03e-71 226
MS.gene068475.t1 AT5G62180 34.505 313 190 10 43 347 21 326 6.40e-50 170
MS.gene068475.t1 AT5G06570 36.897 290 161 6 71 348 50 329 3.64e-45 157
MS.gene068475.t1 AT5G06570 36.897 290 161 6 71 348 50 329 3.64e-45 157
MS.gene068475.t1 AT5G06570 36.897 290 161 6 71 348 65 344 4.59e-45 157
MS.gene068475.t1 AT5G06570 36.897 290 161 6 71 348 65 344 4.59e-45 157
MS.gene068475.t1 AT1G68620 32.404 287 173 7 67 345 57 330 1.62e-43 153
MS.gene068475.t1 AT5G16080 32.500 280 165 6 59 327 54 320 6.73e-43 152
MS.gene068475.t1 AT1G47480 30.303 330 206 8 24 349 5 314 1.16e-42 150
MS.gene068475.t1 AT2G45600 31.973 294 174 7 70 350 39 319 1.29e-42 151
MS.gene068475.t1 AT2G45610 31.529 314 193 8 43 345 22 324 3.92e-39 141
MS.gene068475.t1 AT1G19190 30.696 316 189 11 43 346 18 315 3.57e-34 128
MS.gene068475.t1 AT3G48700 29.934 304 184 8 60 344 32 325 2.68e-33 126
MS.gene068475.t1 AT2G03550 31.544 298 176 8 60 346 32 312 8.92e-33 124
MS.gene068475.t1 AT3G48690 28.428 299 190 7 60 344 32 320 1.26e-27 110
MS.gene068475.t1 AT3G27320 26.590 346 174 7 58 324 48 392 1.46e-27 112
MS.gene068475.t1 AT5G14310 29.603 277 148 5 103 335 148 421 3.99e-27 111
MS.gene068475.t1 AT1G49660 29.365 252 162 5 64 308 36 278 8.10e-26 105
MS.gene068475.t1 AT3G27320 28.912 294 159 7 83 335 151 435 1.12e-25 107
MS.gene068475.t1 AT1G49640 32.812 192 120 4 48 238 23 206 6.09e-25 103
MS.gene068475.t1 AT1G49650 29.804 255 162 6 64 311 92 336 2.93e-22 97.1

Find 102 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGGGCTTCCAAGGAATA+AGG 0.269212 6.3:-65095182 None:intergenic
TGCGGAGGATTTGTCGGGTT+TGG 0.280062 6.3:+65095941 MS.gene068475:CDS
GACGGGAAGGGAGGTTGTTT+TGG 0.283048 6.3:-65095427 None:intergenic
AGACAGTGCCGATGCAAATT+TGG 0.285174 6.3:+65095692 MS.gene068475:CDS
GGGGTTAACGGTAACGTCTT+TGG 0.289367 6.3:-65095334 None:intergenic
GTTGAAGAAATGTGGGAAAA+AGG 0.337242 6.3:+65096037 MS.gene068475:CDS
GGTTAGATCCTTTAAATGAT+TGG 0.346123 6.3:+65095994 MS.gene068475:CDS
TGGCACATCACCTTGCAGTT+AGG 0.348786 6.3:+65095712 MS.gene068475:CDS
CAACAACAGCGGAGATTTGC+CGG 0.365940 6.3:-65095530 None:intergenic
CTCCAGGAAATTCGGATTAT+CGG 0.370807 6.3:+65095750 MS.gene068475:CDS
TGAGTAGATTCAGGCAAATC+AGG 0.377775 6.3:-65096107 None:intergenic
GGCAAAAGACGTATTATGAT+TGG 0.383060 6.3:+65096015 MS.gene068475:CDS
GGCACATCACCTTGCAGTTA+GGG 0.384459 6.3:+65095713 MS.gene068475:CDS
AAGGGATCGAACCGGGATCA+TGG 0.385021 6.3:+65095894 MS.gene068475:CDS
CCGTTAACGGGAGTGGCATT+AGG 0.400101 6.3:-65095303 None:intergenic
AAGCAGCGCTCCAGGAAATT+CGG 0.405027 6.3:+65095742 MS.gene068475:CDS
GCCGAATGCGGAGGATTTGT+CGG 0.410434 6.3:+65095935 MS.gene068475:CDS
AACCTAAACCCCTTATTCCT+TGG 0.412248 6.3:+65095172 MS.gene068475:CDS
TTCCAAGGAATAAGGGGTTT+AGG 0.417633 6.3:-65095174 None:intergenic
TGACGTGTCAAAATGTTTCC+TGG 0.421557 6.3:+65095665 MS.gene068475:CDS
TTAACCCCGAAAATAATGTT+TGG 0.422380 6.3:+65095349 MS.gene068475:CDS
CATGGGGCAGTTAATGTAAA+CGG 0.422557 6.3:+65095912 MS.gene068475:CDS
GCGGGGCTTCCAAGGAATAA+GGG 0.428492 6.3:-65095181 None:intergenic
TTTGGTGGAAACGCCGTTAA+CGG 0.432566 6.3:-65095316 None:intergenic
CCTAATGCCACTCCCGTTAA+CGG 0.433533 6.3:+65095303 MS.gene068475:CDS
AGGGATCGAACCGGGATCAT+GGG 0.438059 6.3:+65095895 MS.gene068475:CDS
CCGAATGCGGAGGATTTGTC+GGG 0.438724 6.3:+65095936 MS.gene068475:CDS
TCCAGGAAATTCGGATTATC+GGG 0.444345 6.3:+65095751 MS.gene068475:CDS
CCCGACAAATCCTCCGCATT+CGG 0.445071 6.3:-65095936 None:intergenic
GGTTTATAAAGCAGCGCTCC+AGG 0.446788 6.3:+65095734 MS.gene068475:CDS
CTCTTTACACCCACTGTTGG+CGG 0.454181 6.3:+65095378 MS.gene068475:CDS
TTGGTGGAAACGCCGTTAAC+GGG 0.454826 6.3:-65095315 None:intergenic
TGGCATTAGGTGAGGATTTG+CGG 0.456562 6.3:-65095290 None:intergenic
ACCCGATAATCCGAATTTCC+TGG 0.463361 6.3:-65095752 None:intergenic
AATAATCAATCATTTAGAAC+AGG 0.469700 6.3:-65096168 None:intergenic
GAGGCGGAGATTAAACTTGA+TGG 0.470210 6.3:+65095816 MS.gene068475:CDS
ATAATCAATTGAGTAGATTC+AGG 0.472084 6.3:-65096116 None:intergenic
ATATTTGGATGAGAATAAAA+CGG 0.477006 6.3:+65095626 MS.gene068475:CDS
AGATTTGCCGGCAGAGTCTA+CGG 0.494073 6.3:-65095518 None:intergenic
CATTAACTGCCCCATGATCC+CGG 0.496678 6.3:-65095905 None:intergenic
TTTACCGAAGGGATCGAACC+GGG 0.502621 6.3:+65095887 MS.gene068475:CDS
ATGGGGCAGTTAATGTAAAC+GGG 0.502929 6.3:+65095913 MS.gene068475:CDS
TATGATTGGTTGAAGAAATG+TGG 0.503360 6.3:+65096029 MS.gene068475:CDS
ATGGTAGCGCGGGGCTTCCA+AGG 0.509543 6.3:-65095189 None:intergenic
GGAAACGCCGTTAACGGGAG+TGG 0.513035 6.3:-65095310 None:intergenic
TGATCCCGGTTCGATCCCTT+CGG 0.517080 6.3:-65095891 None:intergenic
CATCGGCACTGTCTCCAGCC+AGG 0.526327 6.3:-65095683 None:intergenic
TGGTAAGAACGGATTGGTGG+TGG 0.530541 6.3:+65095856 MS.gene068475:CDS
CGTCTCTTTACACCCACTGT+TGG 0.533230 6.3:+65095375 MS.gene068475:CDS
AAGAAGTGAAATGGTAGCGC+GGG 0.536287 6.3:-65095199 None:intergenic
GAAGAAAATGACGACGGGAA+GGG 0.539782 6.3:-65095439 None:intergenic
TGGATCGCCGTTGGTGTCGA+TGG 0.540273 6.3:+65095836 MS.gene068475:CDS
GGAAGAAAATGACGACGGGA+AGG 0.541051 6.3:-65095440 None:intergenic
GGAAATTCGGATTATCGGGT+TGG 0.547011 6.3:+65095755 MS.gene068475:CDS
GGCGGAGAAGTCATCGGAGA+TGG 0.551507 6.3:+65095396 MS.gene068475:CDS
ATGATTGGTTGAAGAAATGT+GGG 0.557528 6.3:+65096030 MS.gene068475:CDS
GATGACTTCTCCGCCAACAG+TGG 0.558808 6.3:-65095388 None:intergenic
AAAAGCATCATAGTAGATTG+AGG 0.560272 6.3:-65095493 None:intergenic
GTTAACGGTAACGTCTTTGG+TGG 0.561453 6.3:-65095331 None:intergenic
GGGATCGAACCGGGATCATG+GGG 0.562386 6.3:+65095896 MS.gene068475:CDS
GTGTCAAAATGTTTCCTGGC+TGG 0.564698 6.3:+65095669 MS.gene068475:CDS
TAAACTTGATGGATCGCCGT+TGG 0.573185 6.3:+65095827 MS.gene068475:CDS
TGGCGGTGAGGAACGGACTG+AGG 0.575528 6.3:+65095797 MS.gene068475:CDS
CGGGGCTTCCAAGGAATAAG+GGG 0.580014 6.3:-65095180 None:intergenic
TGTAAACGGGCCGAATGCGG+AGG 0.580158 6.3:+65095926 MS.gene068475:CDS
TCAATTGATTATGCAAGTGA+AGG 0.583815 6.3:+65096127 MS.gene068475:CDS
CGATGGTAAGAACGGATTGG+TGG 0.590471 6.3:+65095853 MS.gene068475:CDS
ATGACTTCTCCGCCAACAGT+GGG 0.591641 6.3:-65095387 None:intergenic
TAATGTAAACGGGCCGAATG+CGG 0.592555 6.3:+65095923 MS.gene068475:CDS
GATAGCGATGCTCCGGTGTG+AGG 0.593547 6.3:-65095569 None:intergenic
TGTGACGGATAGCGATGCTC+CGG 0.595092 6.3:-65095576 None:intergenic
GCATCATAGTAGATTGAGGA+GGG 0.595871 6.3:-65095489 None:intergenic
CCGTGGAAGAAAATGACGAC+GGG 0.598794 6.3:-65095444 None:intergenic
AGCGATGCTCCGGTGTGAGG+CGG 0.600306 6.3:-65095566 None:intergenic
AACGGGAGTGGCATTAGGTG+AGG 0.601408 6.3:-65095298 None:intergenic
ACTGTTGGCGGAGAAGTCAT+CGG 0.604892 6.3:+65095390 MS.gene068475:CDS
CGGTGAGGAACGGACTGAGG+CGG 0.607879 6.3:+65095800 MS.gene068475:CDS
AAGAACGGATTGGTGGTGGA+AGG 0.610997 6.3:+65095860 MS.gene068475:CDS
CAGCAAAATCATGATAGATG+AGG 0.613047 6.3:+65095116 None:intergenic
CTTTATAAACCCTAACTGCA+AGG 0.618028 6.3:-65095722 None:intergenic
GAAAATGACGACGGGAAGGG+AGG 0.620487 6.3:-65095436 None:intergenic
GACGCTTGTTGTCGCTCTAA+CGG 0.621428 6.3:+65095234 MS.gene068475:CDS
GTTGGTGTCGATGGTAAGAA+CGG 0.628389 6.3:+65095845 MS.gene068475:CDS
GGAGAAGTCATCGGAGATGG+TGG 0.629162 6.3:+65095399 MS.gene068475:CDS
CAACAGTGGGTGTAAAGAGA+CGG 0.629445 6.3:-65095374 None:intergenic
GTGATGTGCCAAATTTGCAT+CGG 0.629822 6.3:-65095700 None:intergenic
GTTGATTGAATATCCAAACA+TGG 0.630966 6.3:+65096064 MS.gene068475:CDS
GTTCTTACCATCGACACCAA+CGG 0.640241 6.3:-65095843 None:intergenic
GTCACAATACGATGACGGAG+AGG 0.656500 6.3:+65095593 MS.gene068475:CDS
TGTCGATGGTAAGAACGGAT+TGG 0.659666 6.3:+65095850 MS.gene068475:CDS
TCTCCGTCATCGTATTGTGA+CGG 0.668882 6.3:-65095591 None:intergenic
CATCATAGTAGATTGAGGAG+GGG 0.673790 6.3:-65095488 None:intergenic
AAAGAAGTGAAATGGTAGCG+CGG 0.678287 6.3:-65095200 None:intergenic
TATCCGTCACAATACGATGA+CGG 0.680205 6.3:+65095588 MS.gene068475:CDS
AGCATCATAGTAGATTGAGG+AGG 0.682743 6.3:-65095490 None:intergenic
ACCGTGGAAGAAAATGACGA+CGG 0.693683 6.3:-65095445 None:intergenic
AGAAGTGAAATGGTAGCGCG+GGG 0.693711 6.3:-65095198 None:intergenic
TGTCAACTACCGCCTCACAC+CGG 0.695639 6.3:+65095557 MS.gene068475:CDS
TGAAGAAGTTAAAGAGACGA+CGG 0.697225 6.3:-65095266 None:intergenic
GTTAGAGCGACAACAAGCGT+CGG 0.706955 6.3:-65095232 None:intergenic
GATAGTTGAAGCCACCACCG+TGG 0.715381 6.3:-65095461 None:intergenic
GTTGACAGAGACAACAACAG+CGG 0.758726 6.3:-65095541 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGGATGAGAATAAAA+CGG + chr6.3:65095626-65095645 MS.gene068475:CDS 20.0%
!! TAAAAATGAAAGAAGTGAAA+TGG - chr6.3:65095211-65095230 None:intergenic 20.0%
! ATAATCAATTGAGTAGATTC+AGG - chr6.3:65096119-65096138 None:intergenic 25.0%
CCAATAAAAACAAGCGTATT+CGG - chr6.3:65095972-65095991 None:intergenic 30.0%
GGTTAGATCCTTTAAATGAT+TGG + chr6.3:65095994-65096013 MS.gene068475:CDS 30.0%
GTTGATTGAATATCCAAACA+TGG + chr6.3:65096064-65096083 MS.gene068475:CDS 30.0%
TCAATTGATTATGCAAGTGA+AGG + chr6.3:65096127-65096146 MS.gene068475:CDS 30.0%
TTAACCCCGAAAATAATGTT+TGG + chr6.3:65095349-65095368 MS.gene068475:CDS 30.0%
! ACGGAACCAAACATTATTTT+CGG - chr6.3:65095358-65095377 None:intergenic 30.0%
! ATGATTGGTTGAAGAAATGT+GGG + chr6.3:65096030-65096049 MS.gene068475:CDS 30.0%
! GTCTTTTGCCAATCATTTAA+AGG - chr6.3:65096005-65096024 None:intergenic 30.0%
! TAAAAAGCATGAACCATGTT+TGG - chr6.3:65096080-65096099 None:intergenic 30.0%
! TATGATTGGTTGAAGAAATG+TGG + chr6.3:65096029-65096048 MS.gene068475:CDS 30.0%
! TGAGAATAAAACGGTTTTAC+CGG + chr6.3:65095635-65095654 MS.gene068475:CDS 30.0%
!! AAAAGCATCATAGTAGATTG+AGG - chr6.3:65095496-65095515 None:intergenic 30.0%
!! AACATTATTTTCGGGGTTAA+CGG - chr6.3:65095349-65095368 None:intergenic 30.0%
!!! AATACGCTTGTTTTTATTGG+TGG + chr6.3:65095972-65095991 MS.gene068475:CDS 30.0%
!!! AGAGGCTGTTTTGAAATATT+TGG + chr6.3:65095611-65095630 MS.gene068475:CDS 30.0%
!!! ATACGCTTGTTTTTATTGGT+GGG + chr6.3:65095973-65095992 MS.gene068475:CDS 30.0%
CTTTATAAACCCTAACTGCA+AGG - chr6.3:65095725-65095744 None:intergenic 35.0%
GGCAAAAGACGTATTATGAT+TGG + chr6.3:65096015-65096034 MS.gene068475:CDS 35.0%
GTTGAAGAAATGTGGGAAAA+AGG + chr6.3:65096037-65096056 MS.gene068475:CDS 35.0%
TGAAGAAGTTAAAGAGACGA+CGG - chr6.3:65095269-65095288 None:intergenic 35.0%
! CGGAACCAAACATTATTTTC+GGG - chr6.3:65095357-65095376 None:intergenic 35.0%
! GGAACCAAACATTATTTTCG+GGG - chr6.3:65095356-65095375 None:intergenic 35.0%
!!! CCGAATACGCTTGTTTTTAT+TGG + chr6.3:65095969-65095988 MS.gene068475:CDS 35.0%
AACCTAAACCCCTTATTCCT+TGG + chr6.3:65095172-65095191 MS.gene068475:CDS 40.0%
AGCATCATAGTAGATTGAGG+AGG - chr6.3:65095493-65095512 None:intergenic 40.0%
ATGGGGCAGTTAATGTAAAC+GGG + chr6.3:65095913-65095932 MS.gene068475:CDS 40.0%
CATCATAGTAGATTGAGGAG+GGG - chr6.3:65095491-65095510 None:intergenic 40.0%
CATGGGGCAGTTAATGTAAA+CGG + chr6.3:65095912-65095931 MS.gene068475:CDS 40.0%
CTCCAGGAAATTCGGATTAT+CGG + chr6.3:65095750-65095769 MS.gene068475:CDS 40.0%
GCATCATAGTAGATTGAGGA+GGG - chr6.3:65095492-65095511 None:intergenic 40.0%
GTGATGTGCCAAATTTGCAT+CGG - chr6.3:65095703-65095722 None:intergenic 40.0%
TATCCGTCACAATACGATGA+CGG + chr6.3:65095588-65095607 MS.gene068475:CDS 40.0%
TCCAGGAAATTCGGATTATC+GGG + chr6.3:65095751-65095770 MS.gene068475:CDS 40.0%
TGACGTGTCAAAATGTTTCC+TGG + chr6.3:65095665-65095684 MS.gene068475:CDS 40.0%
TGAGTAGATTCAGGCAAATC+AGG - chr6.3:65096110-65096129 None:intergenic 40.0%
TTCCAAGGAATAAGGGGTTT+AGG - chr6.3:65095177-65095196 None:intergenic 40.0%
! AAAGAAGTGAAATGGTAGCG+CGG - chr6.3:65095203-65095222 None:intergenic 40.0%
! TTTGACACGTCAGCATTTTC+CGG - chr6.3:65095657-65095676 None:intergenic 40.0%
!!! GTATCGATTCAGCCGTTTTT+TGG + chr6.3:65095777-65095796 MS.gene068475:CDS 40.0%
!!! TGGAAGGTGTTTTTACCGAA+GGG + chr6.3:65095876-65095895 MS.gene068475:CDS 40.0%
ACCCGATAATCCGAATTTCC+TGG - chr6.3:65095755-65095774 None:intergenic 45.0%
ACCGTGGAAGAAAATGACGA+CGG - chr6.3:65095448-65095467 None:intergenic 45.0%
CAACAGTGGGTGTAAAGAGA+CGG - chr6.3:65095377-65095396 None:intergenic 45.0%
GAAGAAAATGACGACGGGAA+GGG - chr6.3:65095442-65095461 None:intergenic 45.0%
GAGGCGGAGATTAAACTTGA+TGG + chr6.3:65095816-65095835 MS.gene068475:CDS 45.0%
GGAAATTCGGATTATCGGGT+TGG + chr6.3:65095755-65095774 MS.gene068475:CDS 45.0%
GTGTCAAAATGTTTCCTGGC+TGG + chr6.3:65095669-65095688 MS.gene068475:CDS 45.0%
GTTAACGGTAACGTCTTTGG+TGG - chr6.3:65095334-65095353 None:intergenic 45.0%
GTTCTTACCATCGACACCAA+CGG - chr6.3:65095846-65095865 None:intergenic 45.0%
GTTGACAGAGACAACAACAG+CGG - chr6.3:65095544-65095563 None:intergenic 45.0%
TAATGTAAACGGGCCGAATG+CGG + chr6.3:65095923-65095942 MS.gene068475:CDS 45.0%
TCTCCGTCATCGTATTGTGA+CGG - chr6.3:65095594-65095613 None:intergenic 45.0%
TGGCATTAGGTGAGGATTTG+CGG - chr6.3:65095293-65095312 None:intergenic 45.0%
TGTCGATGGTAAGAACGGAT+TGG + chr6.3:65095850-65095869 MS.gene068475:CDS 45.0%
TTTGGTGGAAACGCCGTTAA+CGG - chr6.3:65095319-65095338 None:intergenic 45.0%
! AAGAAGTGAAATGGTAGCGC+GGG - chr6.3:65095202-65095221 None:intergenic 45.0%
! TAAACTTGATGGATCGCCGT+TGG + chr6.3:65095827-65095846 MS.gene068475:CDS 45.0%
! TTTTACCGAAGGGATCGAAC+CGG + chr6.3:65095886-65095905 MS.gene068475:CDS 45.0%
!! AGACAGTGCCGATGCAAATT+TGG + chr6.3:65095692-65095711 MS.gene068475:CDS 45.0%
!! GTTGGTGTCGATGGTAAGAA+CGG + chr6.3:65095845-65095864 MS.gene068475:CDS 45.0%
!!! GTGGAAGGTGTTTTTACCGA+AGG + chr6.3:65095875-65095894 MS.gene068475:CDS 45.0%
!!! TCGATTCAGCCGTTTTTTGG+CGG + chr6.3:65095780-65095799 MS.gene068475:CDS 45.0%
AAGCAGCGCTCCAGGAAATT+CGG + chr6.3:65095742-65095761 MS.gene068475:CDS 50.0%
ACTGTTGGCGGAGAAGTCAT+CGG + chr6.3:65095390-65095409 MS.gene068475:CDS 50.0%
AGATTTGCCGGCAGAGTCTA+CGG - chr6.3:65095521-65095540 None:intergenic 50.0%
ATGACTTCTCCGCCAACAGT+GGG - chr6.3:65095390-65095409 None:intergenic 50.0%
CAACAACAGCGGAGATTTGC+CGG - chr6.3:65095533-65095552 None:intergenic 50.0%
CATTAACTGCCCCATGATCC+CGG - chr6.3:65095908-65095927 None:intergenic 50.0%
CCGTGGAAGAAAATGACGAC+GGG - chr6.3:65095447-65095466 None:intergenic 50.0%
CCTAATGCCACTCCCGTTAA+CGG + chr6.3:65095303-65095322 MS.gene068475:CDS 50.0%
CGTCTCTTTACACCCACTGT+TGG + chr6.3:65095375-65095394 MS.gene068475:CDS 50.0%
CGTTCCTCACCGCCAAAAAA+CGG - chr6.3:65095792-65095811 None:intergenic 50.0%
CTCTTTACACCCACTGTTGG+CGG + chr6.3:65095378-65095397 MS.gene068475:CDS 50.0%
GGAAGAAAATGACGACGGGA+AGG - chr6.3:65095443-65095462 None:intergenic 50.0%
GGCACATCACCTTGCAGTTA+GGG + chr6.3:65095713-65095732 MS.gene068475:CDS 50.0%
GGGGTTAACGGTAACGTCTT+TGG - chr6.3:65095337-65095356 None:intergenic 50.0%
GGTTTATAAAGCAGCGCTCC+AGG + chr6.3:65095734-65095753 MS.gene068475:CDS 50.0%
GTTAGAGCGACAACAAGCGT+CGG - chr6.3:65095235-65095254 None:intergenic 50.0%
TGGCACATCACCTTGCAGTT+AGG + chr6.3:65095712-65095731 MS.gene068475:CDS 50.0%
TTGGTGGAAACGCCGTTAAC+GGG - chr6.3:65095318-65095337 None:intergenic 50.0%
TTTACCGAAGGGATCGAACC+GGG + chr6.3:65095887-65095906 MS.gene068475:CDS 50.0%
! AGAAGTGAAATGGTAGCGCG+GGG - chr6.3:65095201-65095220 None:intergenic 50.0%
! CTTTTTGCCGTAGACTCTGC+CGG + chr6.3:65095511-65095530 MS.gene068475:CDS 50.0%
!! AAGAACGGATTGGTGGTGGA+AGG + chr6.3:65095860-65095879 MS.gene068475:CDS 50.0%
!! CCCGTCGTCATTTTCTTCCA+CGG + chr6.3:65095444-65095463 MS.gene068475:CDS 50.0%
!! CGATGGTAAGAACGGATTGG+TGG + chr6.3:65095853-65095872 MS.gene068475:CDS 50.0%
!! GACGCTTGTTGTCGCTCTAA+CGG + chr6.3:65095234-65095253 MS.gene068475:CDS 50.0%
!! GTCACAATACGATGACGGAG+AGG + chr6.3:65095593-65095612 MS.gene068475:CDS 50.0%
!! GTCATTTTCTTCCACGGTGG+TGG + chr6.3:65095450-65095469 MS.gene068475:CDS 50.0%
!! GTCGTCATTTTCTTCCACGG+TGG + chr6.3:65095447-65095466 MS.gene068475:CDS 50.0%
!! TGGTAAGAACGGATTGGTGG+TGG + chr6.3:65095856-65095875 MS.gene068475:CDS 50.0%
!!! CGTTTTTTGGCGGTGAGGAA+CGG + chr6.3:65095790-65095809 MS.gene068475:CDS 50.0%
AACGGGAGTGGCATTAGGTG+AGG - chr6.3:65095301-65095320 None:intergenic 55.0%
AAGGGATCGAACCGGGATCA+TGG + chr6.3:65095894-65095913 MS.gene068475:CDS 55.0%
AGGGATCGAACCGGGATCAT+GGG + chr6.3:65095895-65095914 MS.gene068475:CDS 55.0%
CCCGACAAATCCTCCGCATT+CGG - chr6.3:65095939-65095958 None:intergenic 55.0%
CCGAATGCGGAGGATTTGTC+GGG + chr6.3:65095936-65095955 MS.gene068475:CDS 55.0%
CCGTTAACGGGAGTGGCATT+AGG - chr6.3:65095306-65095325 None:intergenic 55.0%
CGGGGCTTCCAAGGAATAAG+GGG - chr6.3:65095183-65095202 None:intergenic 55.0%
GAAAATGACGACGGGAAGGG+AGG - chr6.3:65095439-65095458 None:intergenic 55.0%
GATAGTTGAAGCCACCACCG+TGG - chr6.3:65095464-65095483 None:intergenic 55.0%
GATGACTTCTCCGCCAACAG+TGG - chr6.3:65095391-65095410 None:intergenic 55.0%
GCCGAATGCGGAGGATTTGT+CGG + chr6.3:65095935-65095954 MS.gene068475:CDS 55.0%
GCGGGGCTTCCAAGGAATAA+GGG - chr6.3:65095184-65095203 None:intergenic 55.0%
GGAGAAGTCATCGGAGATGG+TGG + chr6.3:65095399-65095418 MS.gene068475:CDS 55.0%
TGCGGAGGATTTGTCGGGTT+TGG + chr6.3:65095941-65095960 MS.gene068475:CDS 55.0%
TGTCAACTACCGCCTCACAC+CGG + chr6.3:65095557-65095576 MS.gene068475:CDS 55.0%
! GACGGGAAGGGAGGTTGTTT+TGG - chr6.3:65095430-65095449 None:intergenic 55.0%
! TGATCCCGGTTCGATCCCTT+CGG - chr6.3:65095894-65095913 None:intergenic 55.0%
!! TGTGACGGATAGCGATGCTC+CGG - chr6.3:65095579-65095598 None:intergenic 55.0%
!!! TCAGCCGTTTTTTGGCGGTG+AGG + chr6.3:65095785-65095804 MS.gene068475:CDS 55.0%
CGCGGGGCTTCCAAGGAATA+AGG - chr6.3:65095185-65095204 None:intergenic 60.0%
GATAGCGATGCTCCGGTGTG+AGG - chr6.3:65095572-65095591 None:intergenic 60.0%
GGAAACGCCGTTAACGGGAG+TGG - chr6.3:65095313-65095332 None:intergenic 60.0%
GGCGGAGAAGTCATCGGAGA+TGG + chr6.3:65095396-65095415 MS.gene068475:CDS 60.0%
GGGATCGAACCGGGATCATG+GGG + chr6.3:65095896-65095915 MS.gene068475:CDS 60.0%
TGTAAACGGGCCGAATGCGG+AGG + chr6.3:65095926-65095945 MS.gene068475:CDS 60.0%
!! TGGATCGCCGTTGGTGTCGA+TGG + chr6.3:65095836-65095855 MS.gene068475:CDS 60.0%
AGCGATGCTCCGGTGTGAGG+CGG - chr6.3:65095569-65095588 None:intergenic 65.0%
CATCGGCACTGTCTCCAGCC+AGG - chr6.3:65095686-65095705 None:intergenic 65.0%
! ATGGTAGCGCGGGGCTTCCA+AGG - chr6.3:65095192-65095211 None:intergenic 65.0%
!! CGGTGAGGAACGGACTGAGG+CGG + chr6.3:65095800-65095819 MS.gene068475:CDS 65.0%
!! TGGCGGTGAGGAACGGACTG+AGG + chr6.3:65095797-65095816 MS.gene068475:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 65095126 65096181 65095126 ID=MS.gene068475
chr6.3 mRNA 65095126 65096181 65095126 ID=MS.gene068475.t1;Parent=MS.gene068475
chr6.3 exon 65095126 65096181 65095126 ID=MS.gene068475.t1.exon1;Parent=MS.gene068475.t1
chr6.3 CDS 65095126 65096181 65095126 ID=cds.MS.gene068475.t1;Parent=MS.gene068475.t1
Gene Sequence

>MS.gene068475

ATGATAGATGAGGACGACAGTATGTCACACTCAAACTCAACAAACAAACCTAAACCCCTTATTCCTTGGAAGCCCCGCGCTACCATTTCACTTCTTTCATTTTTAACCGACGCTTGTTGTCGCTCTAACGGCACATTCAACCGTCGTCTCTTTAACTTCTTCATCCGCAAATCCTCACCTAATGCCACTCCCGTTAACGGCGTTTCCACCAAAGACGTTACCGTTAACCCCGAAAATAATGTTTGGTTCCGTCTCTTTACACCCACTGTTGGCGGAGAAGTCATCGGAGATGGTGGTGCAACCAAAACAACCTCCCTTCCCGTCGTCATTTTCTTCCACGGTGGTGGCTTCAACTATCTTTGCCCCTCCTCAATCTACTATGATGCTTTTTGCCGTAGACTCTGCCGGCAAATCTCCGCTGTTGTTGTCTCTGTCAACTACCGCCTCACACCGGAGCATCGCTATCCGTCACAATACGATGACGGAGAGGCTGTTTTGAAATATTTGGATGAGAATAAAACGGTTTTACCGGAAAATGCTGACGTGTCAAAATGTTTCCTGGCTGGAGACAGTGCCGATGCAAATTTGGCACATCACCTTGCAGTTAGGGTTTATAAAGCAGCGCTCCAGGAAATTCGGATTATCGGGTTGGTATCGATTCAGCCGTTTTTTGGCGGTGAGGAACGGACTGAGGCGGAGATTAAACTTGATGGATCGCCGTTGGTGTCGATGGTAAGAACGGATTGGTGGTGGAAGGTGTTTTTACCGAAGGGATCGAACCGGGATCATGGGGCAGTTAATGTAAACGGGCCGAATGCGGAGGATTTGTCGGGTTTGGATTATCCGAATACGCTTGTTTTTATTGGTGGGTTAGATCCTTTAAATGATTGGCAAAAGACGTATTATGATTGGTTGAAGAAATGTGGGAAAAAGGTTGAGTTGATTGAATATCCAAACATGGTTCATGCTTTTTATATTTTTCCTGATTTGCCTGAATCTACTCAATTGATTATGCAAGTGAAGGAGTTTGTCAACAAAGTCTCCTGTTCTAAATGA
Protein sequence

>MS.gene068475.t1

MIDEDDSMSHSNSTNKPKPLIPWKPRATISLLSFLTDACCRSNGTFNRRLFNFFIRKSSPNATPVNGVSTKDVTVNPENNVWFRLFTPTVGGEVIGDGGATKTTSLPVVIFFHGGGFNYLCPSSIYYDAFCRRLCRQISAVVVSVNYRLTPEHRYPSQYDDGEAVLKYLDENKTVLPENADVSKCFLAGDSADANLAHHLAVRVYKAALQEIRIIGLVSIQPFFGGEERTEAEIKLDGSPLVSMVRTDWWWKVFLPKGSNRDHGAVNVNGPNAEDLSGLDYPNTLVFIGGLDPLNDWQKTYYDWLKKCGKKVELIEYPNMVHAFYIFPDLPESTQLIMQVKEFVNKVSCSK