AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068477

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068477.t1 MTR_6g065400 92.258 310 24 0 1 310 1 310 0.0 602
MS.gene068477.t1 MTR_6g065370 91.613 310 26 0 1 310 1 310 0.0 599
MS.gene068477.t1 MTR_6g065270 80.456 307 56 1 8 310 6 312 0.0 523
MS.gene068477.t1 MTR_6g065120 76.161 323 76 1 2 323 3 325 0.0 513
MS.gene068477.t1 MTR_6g065340 77.994 309 52 1 2 310 13 305 3.44e-170 477
MS.gene068477.t1 MTR_6g065110 70.740 311 91 0 5 315 6 316 3.03e-166 467
MS.gene068477.t1 MTR_6g065430 58.824 306 117 2 7 310 6 304 4.91e-131 378
MS.gene068477.t1 MTR_1g089310 41.748 309 171 3 1 304 7 311 7.45e-76 237
MS.gene068477.t1 MTR_1g089310 41.748 309 171 3 1 304 23 327 8.54e-76 238
MS.gene068477.t1 MTR_1g082210 41.100 309 173 3 1 304 7 311 3.63e-75 236
MS.gene068477.t1 MTR_7g093950 39.423 312 180 4 1 304 7 317 1.01e-72 229
MS.gene068477.t1 MTR_6g464280 38.321 274 155 5 32 298 24 290 3.03e-54 181
MS.gene068477.t1 MTR_6g464510 37.050 278 153 8 31 298 27 292 1.01e-49 169
MS.gene068477.t1 MTR_8g035540 37.453 267 146 5 47 302 30 286 2.78e-49 168
MS.gene068477.t1 MTR_1g102160 36.594 276 138 9 31 287 23 280 2.84e-45 157
MS.gene068477.t1 MTR_8g035520 34.831 267 153 6 49 304 40 296 5.74e-44 154
MS.gene068477.t1 MTR_6g464320 35.401 274 163 5 32 298 25 291 9.85e-44 154
MS.gene068477.t1 MTR_0007s0520 34.579 321 171 11 1 306 1 297 1.01e-43 154
MS.gene068477.t1 MTR_8g035500 32.740 281 169 6 32 302 17 287 3.11e-43 152
MS.gene068477.t1 MTR_4g050140 34.155 284 166 4 31 306 17 287 5.01e-42 149
MS.gene068477.t1 MTR_1g102170 36.134 238 123 6 32 260 43 260 1.28e-41 148
MS.gene068477.t1 MTR_6g464120 35.000 240 132 6 32 264 29 251 2.37e-41 151
MS.gene068477.t1 MTR_1g012430 35.424 271 157 6 31 298 15 270 6.42e-41 145
MS.gene068477.t1 MTR_7g107040 32.899 307 174 6 10 306 4 288 2.72e-40 144
MS.gene068477.t1 MTR_7g406880 35.793 271 149 8 55 306 54 318 2.75e-39 142
MS.gene068477.t1 MTR_1g111280 33.210 271 150 9 48 302 52 307 8.48e-37 136
MS.gene068477.t1 MTR_3g012440 32.103 271 162 3 48 304 41 303 2.62e-34 129
MS.gene068477.t1 MTR_4g086540 31.970 269 148 6 32 298 31 266 6.17e-33 125
MS.gene068477.t1 MTR_4g086480 31.274 259 152 5 49 298 39 280 5.66e-32 122
MS.gene068477.t1 MTR_4g086510 32.967 273 148 7 49 305 35 288 7.55e-32 122
MS.gene068477.t1 MTR_7g056207 32.432 259 152 5 49 298 67 311 2.76e-31 121
MS.gene068477.t1 MTR_3g101420 30.855 269 157 5 49 305 34 285 7.45e-31 119
MS.gene068477.t1 MTR_6g022710 32.180 289 149 7 95 340 160 444 1.22e-30 121
MS.gene068477.t1 MTR_4g086470 30.888 259 153 5 49 298 38 279 9.83e-30 116
MS.gene068477.t1 MTR_5g005920 29.474 285 177 9 31 303 25 297 1.82e-29 115
MS.gene068477.t1 MTR_3g450680 28.889 270 154 7 53 303 48 298 4.19e-25 103
MS.gene068477.t1 MTR_5g005850 29.545 264 160 7 53 303 45 295 4.49e-25 103
MS.gene068477.t1 MTR_1g105020 28.223 287 173 9 32 303 21 289 9.59e-25 102
MS.gene068477.t1 MTR_6g065470 35.088 171 79 3 7 159 6 162 1.46e-23 95.9
MS.gene068477.t1 MTR_8g040860 26.531 294 169 9 31 303 28 295 1.55e-23 99.8
MS.gene068477.t1 MTR_5g005900 27.368 285 169 10 31 296 21 286 2.58e-23 99.0
MS.gene068477.t1 MTR_1g104930 30.435 299 163 9 23 302 16 288 3.16e-23 98.6
MS.gene068477.t1 MTR_7g051020 29.766 299 170 8 23 303 16 292 1.30e-22 97.1
MS.gene068477.t1 MTR_1g104933 30.980 255 147 6 59 302 46 282 5.90e-20 89.4
MS.gene068477.t1 MTR_6g065480 53.571 56 25 1 235 290 3 57 6.51e-15 68.9
MS.gene068477.t1 MTR_0007s0480 34.000 150 78 6 171 306 1 143 7.41e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068477.t1 AT5G23530 54.717 318 126 4 10 323 13 316 1.77e-127 369
MS.gene068477.t1 AT5G27320 42.525 301 167 2 7 304 13 310 6.60e-78 243
MS.gene068477.t1 AT3G63010 40.968 310 173 4 1 304 7 312 6.75e-73 230
MS.gene068477.t1 AT3G05120 40.193 311 164 3 7 305 13 313 1.19e-70 224
MS.gene068477.t1 AT5G62180 37.367 281 164 7 32 306 21 295 7.44e-55 182
MS.gene068477.t1 AT2G45600 33.721 258 148 4 58 302 38 285 1.99e-45 158
MS.gene068477.t1 AT2G45610 30.868 311 188 6 1 302 1 293 4.93e-43 152
MS.gene068477.t1 AT5G16080 34.444 270 153 6 48 306 54 310 8.91e-43 151
MS.gene068477.t1 AT1G68620 37.021 235 132 5 56 285 57 280 4.80e-41 147
MS.gene068477.t1 AT5G06570 38.846 260 138 5 60 308 50 299 2.22e-39 142
MS.gene068477.t1 AT5G06570 38.846 260 138 5 60 308 50 299 2.22e-39 142
MS.gene068477.t1 AT5G06570 38.846 260 138 5 60 308 65 314 2.69e-39 142
MS.gene068477.t1 AT5G06570 38.846 260 138 5 60 308 65 314 2.69e-39 142
MS.gene068477.t1 AT1G19190 32.971 276 158 8 32 298 18 275 1.37e-34 129
MS.gene068477.t1 AT5G14310 31.399 293 151 6 95 340 151 440 6.04e-33 127
MS.gene068477.t1 AT3G48700 30.882 272 163 6 49 305 32 293 4.14e-32 123
MS.gene068477.t1 AT2G03550 33.840 263 149 6 49 303 32 277 3.73e-31 120
MS.gene068477.t1 AT1G47480 30.363 303 171 9 9 305 12 280 3.85e-31 120
MS.gene068477.t1 AT3G48690 32.090 268 151 6 49 300 32 284 3.56e-30 117
MS.gene068477.t1 AT3G27320 28.024 339 164 6 47 306 48 385 8.27e-30 118
MS.gene068477.t1 AT3G27320 29.431 299 161 6 72 329 151 440 1.47e-28 115
MS.gene068477.t1 AT1G49640 31.746 189 119 5 30 217 17 196 1.21e-25 105
MS.gene068477.t1 AT1G49660 31.349 252 157 7 53 297 36 278 1.59e-25 105
MS.gene068477.t1 AT1G49650 30.078 256 162 6 53 301 92 337 2.24e-24 102

Find 109 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGAGGATTTGTCGGGTT+TGG 0.228528 6.3:+65147256 MS.gene068477:CDS
ACCCGATAACCCGGATATTC+TGG 0.265541 6.3:-65147067 None:intergenic
AGATAGCGCCGGTGGAAATT+TGG 0.269758 6.3:+65147007 MS.gene068477:CDS
GTCGTTAACGGTGATGTCTT+TGG 0.284165 6.3:-65146649 None:intergenic
CTCTTTACTCCCACTGTTGC+CGG 0.296074 6.3:+65146693 MS.gene068477:CDS
CTTCCAAGGAAGAAGGGGTT+TGG 0.297350 6.3:-65146490 None:intergenic
CAAAAGTCTTCTAGCCAAAA+TGG 0.329107 6.3:-65147856 None:intergenic
GCGGTTAGGGCTTGCAAAGC+GGG 0.337635 6.3:+65147041 MS.gene068477:CDS
ATGGTCCCGGTCCGATCCTT+CGG 0.344521 6.3:-65147207 None:intergenic
ACAGTAGATGATGACCATTT+TGG 0.348137 6.3:+65147842 MS.gene068477:intron
GACAGGAAGGGAGATTGTTT+TGG 0.351128 6.3:-65146742 None:intergenic
GGCACACCATGTTGCGGTTA+GGG 0.353563 6.3:+65147028 MS.gene068477:CDS
CTCCAGAATATCCGGGTTAT+CGG 0.354754 6.3:+65147065 MS.gene068477:CDS
GGTTTGATCCTTTGATTGAT+TGG 0.363142 6.3:+65147309 MS.gene068477:CDS
TGGCACACCATGTTGCGGTT+AGG 0.375335 6.3:+65147027 MS.gene068477:CDS
AACCCAAACCCCTTCTTCCT+TGG 0.375869 6.3:+65146487 MS.gene068477:CDS
GGCAAAAGAGGTATTATGAT+TGG 0.376841 6.3:+65147330 MS.gene068477:CDS
TGAAAAGCATGAACCATGTT+TGG 0.377355 6.3:-65147392 None:intergenic
CGACGACAGCAGAGATTTCC+CGG 0.388643 6.3:-65146845 None:intergenic
CCTAACACCACTCCCGTTAA+CGG 0.390634 6.3:+65146618 MS.gene068477:CDS
AAGCGGGGCTCCAGAATATC+CGG 0.405006 6.3:+65147057 MS.gene068477:CDS
TTCCAAGGAAGAAGGGGTTT+GGG 0.406480 6.3:-65146489 None:intergenic
CCGTTAACGGGAGTGGTGTT+AGG 0.419725 6.3:-65146618 None:intergenic
GCCGAACGCGGAGGATTTGT+CGG 0.420668 6.3:+65147250 MS.gene068477:CDS
TTTGGTGGAAACACCGTTAA+CGG 0.431078 6.3:-65146631 None:intergenic
CCCGACAAATCCTCCGCGTT+CGG 0.434640 6.3:-65147251 None:intergenic
TGGTCCCGGTCCGATCCTTC+GGG 0.435638 6.3:-65147206 None:intergenic
TTGGTGGAAACACCGTTAAC+GGG 0.448936 6.3:-65146630 None:intergenic
TTTGACAAGTCAGCATTTCC+CGG 0.449205 6.3:-65146969 None:intergenic
CCGAACGCGGAGGATTTGTC+GGG 0.451420 6.3:+65147251 MS.gene068477:CDS
TTTGTCGGGTTTGGATTATC+CGG 0.455467 6.3:+65147265 MS.gene068477:CDS
CTGTTTGCCGTAGACTCTGC+CGG 0.459198 6.3:+65146826 MS.gene068477:CDS
TAAAACTGTAAGAAGTGAAA+TGG 0.461001 6.3:-65146523 None:intergenic
TTAACGACGAAAATAACGTT+TGG 0.461518 6.3:+65146664 MS.gene068477:CDS
TCCAGAATATCCGGGTTATC+GGG 0.461680 6.3:+65147066 MS.gene068477:CDS
AACAGCATCGTAGAGGTTAG+AGG 0.470736 6.3:-65146808 None:intergenic
ATATTGATCCAACCGTTCTT+CGG 0.477926 6.3:+65147092 MS.gene068477:CDS
CATGGCGCGGTTAATGTATG+TGG 0.478399 6.3:+65147227 MS.gene068477:CDS
AGGAGAAGAACGGACGGAGG+CGG 0.478435 6.3:+65147115 MS.gene068477:CDS
CGCGGACCTTCCAAGGAAGA+AGG 0.480629 6.3:-65146497 None:intergenic
CGTTCTTCTCCTCCGAAGAA+CGG 0.480969 6.3:-65147104 None:intergenic
TGCCGTTTGTGTCGGTGGCA+AGG 0.481183 6.3:+65147156 MS.gene068477:CDS
TTTGGTGGAACCACCATCTC+CGG 0.486902 6.3:-65146724 None:intergenic
CTTTGATTGATTGGCAAAAG+AGG 0.488937 6.3:+65147318 MS.gene068477:CDS
CTTCTCCTCCGAAGAACGGT+TGG 0.489442 6.3:-65147100 None:intergenic
TGTCGGTGGCAAGGACTGAT+TGG 0.490643 6.3:+65147165 MS.gene068477:CDS
AGATTTCCCGGCAGAGTCTA+CGG 0.500283 6.3:-65146833 None:intergenic
AGGCGGAGATTCATCTAGTT+GGG 0.501879 6.3:+65147132 MS.gene068477:CDS
AATTTGATTACAAGAACAGT+TGG 0.505669 6.3:+65147927 MS.gene068477:CDS
GAGGCGGAGATTCATCTAGT+TGG 0.506923 6.3:+65147131 MS.gene068477:CDS
CATTAACCGCGCCATGGTCC+CGG 0.510284 6.3:-65147220 None:intergenic
TGGTGCAAGTCGGTAGTTGA+CGG 0.516831 6.3:-65146871 None:intergenic
ATGGTAGCGCGGACCTTCCA+AGG 0.517263 6.3:-65146504 None:intergenic
TGCGACGGATAGCGATGCTC+TGG 0.519802 6.3:-65146891 None:intergenic
GTCCTTGCCACCGACACAAA+CGG 0.526350 6.3:-65147158 None:intergenic
ATTTCCACCGGCGCTATCTC+CGG 0.529772 6.3:-65147003 None:intergenic
TTGTCAAAATGTTTCCTAGC+CGG 0.531131 6.3:+65146984 MS.gene068477:CDS
GCCGGAGAAGTCACCGGAGA+TGG 0.534767 6.3:+65146711 MS.gene068477:CDS
GGAAACACCGTTAACGGGAG+TGG 0.541054 6.3:-65146625 None:intergenic
GCGGACCTTCCAAGGAAGAA+GGG 0.545035 6.3:-65146496 None:intergenic
GGTGACTTCTCCGGCAACAG+TGG 0.548822 6.3:-65146703 None:intergenic
CAAACCCCTTCTTCCTTGGA+AGG 0.548828 6.3:+65146491 MS.gene068477:CDS
TGGGATGCCGTTTGTGTCGG+TGG 0.557749 6.3:+65147151 MS.gene068477:CDS
GTTAACGGTGATGTCTTTGG+TGG 0.559373 6.3:-65146646 None:intergenic
GTTAAAGAGACAGCGGTTGA+CGG 0.560255 6.3:-65146574 None:intergenic
TGCGGTTAGGGCTTGCAAAG+CGG 0.562566 6.3:+65147040 MS.gene068477:CDS
TCAATATTGACCCGATAACC+CGG 0.567192 6.3:-65147076 None:intergenic
ACCATCTCCGGTGACTTCTC+CGG 0.568498 6.3:-65146712 None:intergenic
GTGACTTCTCCGGCAACAGT+GGG 0.568706 6.3:-65146702 None:intergenic
CGGTTAGGGCTTGCAAAGCG+GGG 0.572111 6.3:+65147042 MS.gene068477:CDS
ACTGTTGCCGGAGAAGTCAC+CGG 0.572191 6.3:+65146705 MS.gene068477:CDS
TTTACCCGAAGGATCGGACC+GGG 0.573271 6.3:+65147202 MS.gene068477:CDS
GAAGGATCGGACCGGGACCA+TGG 0.578061 6.3:+65147209 MS.gene068477:CDS
TGGCAAGGACTGATTGGATG+TGG 0.582609 6.3:+65147171 MS.gene068477:CDS
GATGCTTCATGTCGCTCTAA+CGG 0.583341 6.3:+65146549 MS.gene068477:CDS
TGTTTGCCGTAGACTCTGCC+GGG 0.585420 6.3:+65146827 MS.gene068477:CDS
AGTTGGGATGCCGTTTGTGT+CGG 0.586039 6.3:+65147148 MS.gene068477:CDS
GGAAGAAAATAATGACAGGA+AGG 0.590861 6.3:-65146755 None:intergenic
AACGGGAGTGGTGTTAGGTG+AGG 0.594957 6.3:-65146613 None:intergenic
CCGTGGAAGAAAATAATGAC+AGG 0.596483 6.3:-65146759 None:intergenic
CACCGGCGCTATCTCCGGCT+AGG 0.596783 6.3:-65146998 None:intergenic
AGCGGGGCTCCAGAATATCC+GGG 0.603800 6.3:+65147058 MS.gene068477:CDS
GGAGAAGTCACCGGAGATGG+TGG 0.603928 6.3:+65146714 MS.gene068477:CDS
CACATACATTAACCGCGCCA+TGG 0.604387 6.3:-65147226 None:intergenic
ATCGGACCGGGACCATGGCG+CGG 0.608936 6.3:+65147214 MS.gene068477:CDS
CTAGCCGGAGATAGCGCCGG+TGG 0.609370 6.3:+65146999 MS.gene068477:CDS
AAATTTGGCACACCATGTTG+CGG 0.611772 6.3:+65147022 MS.gene068477:CDS
TTGATCCAACCGTTCTTCGG+AGG 0.612628 6.3:+65147095 MS.gene068477:CDS
GAAGAAAATAATGACAGGAA+GGG 0.614078 6.3:-65146754 None:intergenic
ATGGTGTGCCAAATTTCCAC+CGG 0.614422 6.3:-65147015 None:intergenic
ATGGCGCGGTTAATGTATGT+GGG 0.615269 6.3:+65147228 MS.gene068477:CDS
TCTCCGTCTTCATATTGCGA+CGG 0.615711 6.3:-65146906 None:intergenic
CGGACCTTCCAAGGAAGAAG+GGG 0.620041 6.3:-65146495 None:intergenic
TGCAAGCCCTAACCGCAACA+TGG 0.630176 6.3:-65147034 None:intergenic
GAAAAGTGAAGCCACCGCCG+TGG 0.638638 6.3:-65146776 None:intergenic
GCATCGTAGAGGTTAGAGGA+AGG 0.641381 6.3:-65146804 None:intergenic
CGAAGAAGTTAAAGAGACAG+CGG 0.642287 6.3:-65146581 None:intergenic
TAATGTATGTGGGCCGAACG+CGG 0.643618 6.3:+65147238 MS.gene068477:CDS
AGCGATGCTCTGGTGCAAGT+CGG 0.647802 6.3:-65146881 None:intergenic
CAACAGTGGGAGTAAAGAGA+CGG 0.651964 6.3:-65146689 None:intergenic
CGTTCTTCGGAGGAGAAGAA+CGG 0.653860 6.3:+65147105 MS.gene068477:CDS
CTTCGGAGGAGAAGAACGGA+CGG 0.656110 6.3:+65147109 MS.gene068477:CDS
TATCCGTCGCAATATGAAGA+CGG 0.658300 6.3:+65146903 MS.gene068477:CDS
GTTGATTGAATATCCAAACA+TGG 0.659983 6.3:+65147379 MS.gene068477:CDS
TACGGCAAACAGCATCGTAG+AGG 0.660757 6.3:-65146815 None:intergenic
CGGAGGAGAAGAACGGACGG+AGG 0.662414 6.3:+65147112 MS.gene068477:CDS
TTCCTAGCCGGAGATAGCGC+CGG 0.669385 6.3:+65146996 MS.gene068477:CDS
TGTATGTGGGCCGAACGCGG+AGG 0.708115 6.3:+65147241 MS.gene068477:CDS
TAAGAAGTGAAATGGTAGCG+CGG 0.709848 6.3:-65146515 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATAATCATAATTTAGAAT+TGG - chr6.3:65147486-65147505 None:intergenic 15.0%
!! GTCTTGAATTTAATAAATCT+GGG + chr6.3:65147674-65147693 MS.gene068477:intron 20.0%
!! TGTCTTGAATTTAATAAATC+TGG + chr6.3:65147673-65147692 MS.gene068477:intron 20.0%
!!! TATGTTTTTAGAGTAAACTT+TGG + chr6.3:65147641-65147660 MS.gene068477:intron 20.0%
! AATTTGATTACAAGAACAGT+TGG + chr6.3:65147927-65147946 MS.gene068477:CDS 25.0%
! TAAAACTGTAAGAAGTGAAA+TGG - chr6.3:65146526-65146545 None:intergenic 25.0%
!! ATGATTGGTTGAAAAAATGT+GGG + chr6.3:65147345-65147364 MS.gene068477:CDS 25.0%
!! CTCTAAAAACATACTCATAT+AGG - chr6.3:65147634-65147653 None:intergenic 25.0%
!! TATGATTGGTTGAAAAAATG+TGG + chr6.3:65147344-65147363 MS.gene068477:CDS 25.0%
GAAGAAAATAATGACAGGAA+GGG - chr6.3:65146757-65146776 None:intergenic 30.0%
GTTGATTGAATATCCAAACA+TGG + chr6.3:65147379-65147398 MS.gene068477:CDS 30.0%
TTAACGACGAAAATAACGTT+TGG + chr6.3:65146664-65146683 MS.gene068477:CDS 30.0%
! GCGTGTTAGAAATTTAGAAT+AGG + chr6.3:65147755-65147774 MS.gene068477:intron 30.0%
!! AACGTTATTTTCGTCGTTAA+CGG - chr6.3:65146664-65146683 None:intergenic 30.0%
!!! ATACACTTGTTTTTGTTGGT+GGG + chr6.3:65147288-65147307 MS.gene068477:CDS 30.0%
ACAATCAACTGAGTAGATTC+AGG - chr6.3:65147434-65147453 None:intergenic 35.0%
ATATTGATCCAACCGTTCTT+CGG + chr6.3:65147092-65147111 MS.gene068477:CDS 35.0%
ATTGACTATTGTGCACAAGT+TGG - chr6.3:65147793-65147812 None:intergenic 35.0%
CAAAAGTCTTCTAGCCAAAA+TGG - chr6.3:65147859-65147878 None:intergenic 35.0%
CCAACAAAAACAAGTGTATC+CGG - chr6.3:65147287-65147306 None:intergenic 35.0%
CTTTGATTGATTGGCAAAAG+AGG + chr6.3:65147318-65147337 MS.gene068477:CDS 35.0%
GGAAGAAAATAATGACAGGA+AGG - chr6.3:65146758-65146777 None:intergenic 35.0%
GGTTTGATCCTTTGATTGAT+TGG + chr6.3:65147309-65147328 MS.gene068477:CDS 35.0%
GTGCACAATAGTCAATTACT+TGG + chr6.3:65147797-65147816 MS.gene068477:intron 35.0%
TAGTCAATTACTTGGTTGTG+TGG + chr6.3:65147805-65147824 MS.gene068477:intron 35.0%
TGAGTAGATTCAGGAAAATC+AGG - chr6.3:65147425-65147444 None:intergenic 35.0%
TTGACTATTGTGCACAAGTT+GGG - chr6.3:65147792-65147811 None:intergenic 35.0%
TTGTCAAAATGTTTCCTAGC+CGG + chr6.3:65146984-65147003 MS.gene068477:CDS 35.0%
! ACAGTAGATGATGACCATTT+TGG + chr6.3:65147842-65147861 MS.gene068477:intron 35.0%
! CATCTACTGTAGTAGACTTT+TGG - chr6.3:65147832-65147851 None:intergenic 35.0%
! CCTGTCATTATTTTCTTCCA+CGG + chr6.3:65146759-65146778 MS.gene068477:CDS 35.0%
! CTCTTTTGCCAATCAATCAA+AGG - chr6.3:65147320-65147339 None:intergenic 35.0%
! TGAAAAGCATGAACCATGTT+TGG - chr6.3:65147395-65147414 None:intergenic 35.0%
!! GGCAAAAGAGGTATTATGAT+TGG + chr6.3:65147330-65147349 MS.gene068477:CDS 35.0%
!!! CATTTTGGCTAGAAGACTTT+TGG + chr6.3:65147857-65147876 MS.gene068477:CDS 35.0%
!!! GATACACTTGTTTTTGTTGG+TGG + chr6.3:65147287-65147306 MS.gene068477:CDS 35.0%
AAATTTGGCACACCATGTTG+CGG + chr6.3:65147022-65147041 MS.gene068477:CDS 40.0%
CCGTGGAAGAAAATAATGAC+AGG - chr6.3:65146762-65146781 None:intergenic 40.0%
CGAAGAAGTTAAAGAGACAG+CGG - chr6.3:65146584-65146603 None:intergenic 40.0%
TAAGCAAACTGCTCATCATC+TGG - chr6.3:65147718-65147737 None:intergenic 40.0%
TATCCGTCGCAATATGAAGA+CGG + chr6.3:65146903-65146922 MS.gene068477:CDS 40.0%
TCAATATTGACCCGATAACC+CGG - chr6.3:65147079-65147098 None:intergenic 40.0%
TCAGTTGATTGTGCAAGTCA+AGG + chr6.3:65147442-65147461 MS.gene068477:intron 40.0%
TGTAGCTAGAACGTACAAGT+TGG + chr6.3:65147557-65147576 MS.gene068477:intron 40.0%
TTTGACAAGTCAGCATTTCC+CGG - chr6.3:65146972-65146991 None:intergenic 40.0%
TTTGGTGGAAACACCGTTAA+CGG - chr6.3:65146634-65146653 None:intergenic 40.0%
TTTGTCGGGTTTGGATTATC+CGG + chr6.3:65147265-65147284 MS.gene068477:CDS 40.0%
! GATGATGAGCAGTTTGCTTA+TGG + chr6.3:65147718-65147737 MS.gene068477:intron 40.0%
! GTCATTATTTTCTTCCACGG+CGG + chr6.3:65146762-65146781 MS.gene068477:CDS 40.0%
! TAAGAAGTGAAATGGTAGCG+CGG - chr6.3:65146518-65146537 None:intergenic 40.0%
! TGAGCAGTTTGCTTATGGTT+TGG + chr6.3:65147723-65147742 MS.gene068477:intron 40.0%
!! AGTGTTTTTACCCGAAGGAT+CGG + chr6.3:65147196-65147215 MS.gene068477:CDS 40.0%
!!! CCGGATACACTTGTTTTTGT+TGG + chr6.3:65147284-65147303 MS.gene068477:CDS 40.0%
!!! TGGAAAGTGTTTTTACCCGA+AGG + chr6.3:65147191-65147210 MS.gene068477:CDS 40.0%
AACAGCATCGTAGAGGTTAG+AGG - chr6.3:65146811-65146830 None:intergenic 45.0%
AGGCGGAGATTCATCTAGTT+GGG + chr6.3:65147132-65147151 MS.gene068477:CDS 45.0%
ATGGCGCGGTTAATGTATGT+GGG + chr6.3:65147228-65147247 MS.gene068477:CDS 45.0%
ATGGTGTGCCAAATTTCCAC+CGG - chr6.3:65147018-65147037 None:intergenic 45.0%
CAACAGTGGGAGTAAAGAGA+CGG - chr6.3:65146692-65146711 None:intergenic 45.0%
CTCCAGAATATCCGGGTTAT+CGG + chr6.3:65147065-65147084 MS.gene068477:CDS 45.0%
GATGCTTCATGTCGCTCTAA+CGG + chr6.3:65146549-65146568 MS.gene068477:CDS 45.0%
GTCGTTAACGGTGATGTCTT+TGG - chr6.3:65146652-65146671 None:intergenic 45.0%
GTTAAAGAGACAGCGGTTGA+CGG - chr6.3:65146577-65146596 None:intergenic 45.0%
GTTAACGGTGATGTCTTTGG+TGG - chr6.3:65146649-65146668 None:intergenic 45.0%
TCCAGAATATCCGGGTTATC+GGG + chr6.3:65147066-65147085 MS.gene068477:CDS 45.0%
TCTCCGTCTTCATATTGCGA+CGG - chr6.3:65146909-65146928 None:intergenic 45.0%
TTCCAAGGAAGAAGGGGTTT+GGG - chr6.3:65146492-65146511 None:intergenic 45.0%
TTGGTGGAAACACCGTTAAC+GGG - chr6.3:65146633-65146652 None:intergenic 45.0%
! AGAAGACTTTTGGACGAGGT+TGG + chr6.3:65147867-65147886 MS.gene068477:CDS 45.0%
! ATTATTTTCTTCCACGGCGG+TGG + chr6.3:65146765-65146784 MS.gene068477:CDS 45.0%
! CGAGAACGCAACAGTTTTAC+CGG + chr6.3:65146950-65146969 MS.gene068477:CDS 45.0%
! GACAGGAAGGGAGATTGTTT+TGG - chr6.3:65146745-65146764 None:intergenic 45.0%
! GAGAACGCAACAGTTTTACC+GGG + chr6.3:65146951-65146970 MS.gene068477:CDS 45.0%
!! ACTTTTGGACGAGGTTGGTT+GGG + chr6.3:65147872-65147891 MS.gene068477:CDS 45.0%
!!! AGGAAGGGAGATTGTTTTGG+TGG - chr6.3:65146742-65146761 None:intergenic 45.0%
AACCCAAACCCCTTCTTCCT+TGG + chr6.3:65146487-65146506 MS.gene068477:CDS 50.0%
ACCCGATAACCCGGATATTC+TGG - chr6.3:65147070-65147089 None:intergenic 50.0%
AGATTTCCCGGCAGAGTCTA+CGG - chr6.3:65146836-65146855 None:intergenic 50.0%
CAAACCCCTTCTTCCTTGGA+AGG + chr6.3:65146491-65146510 MS.gene068477:CDS 50.0%
CACATACATTAACCGCGCCA+TGG - chr6.3:65147229-65147248 None:intergenic 50.0%
CATGGCGCGGTTAATGTATG+TGG + chr6.3:65147227-65147246 MS.gene068477:CDS 50.0%
CCTAACACCACTCCCGTTAA+CGG + chr6.3:65146618-65146637 MS.gene068477:CDS 50.0%
CGTTCTTCGGAGGAGAAGAA+CGG + chr6.3:65147105-65147124 MS.gene068477:CDS 50.0%
CGTTCTTCTCCTCCGAAGAA+CGG - chr6.3:65147107-65147126 None:intergenic 50.0%
CTCTTTACTCCCACTGTTGC+CGG + chr6.3:65146693-65146712 MS.gene068477:CDS 50.0%
CTTCCAAGGAAGAAGGGGTT+TGG - chr6.3:65146493-65146512 None:intergenic 50.0%
GAGGCGGAGATTCATCTAGT+TGG + chr6.3:65147131-65147150 MS.gene068477:CDS 50.0%
GCATCGTAGAGGTTAGAGGA+AGG - chr6.3:65146807-65146826 None:intergenic 50.0%
TAATGTATGTGGGCCGAACG+CGG + chr6.3:65147238-65147257 MS.gene068477:CDS 50.0%
TACGGCAAACAGCATCGTAG+AGG - chr6.3:65146818-65146837 None:intergenic 50.0%
TGGCAAGGACTGATTGGATG+TGG + chr6.3:65147171-65147190 MS.gene068477:CDS 50.0%
TGGTGCAAGTCGGTAGTTGA+CGG - chr6.3:65146874-65146893 None:intergenic 50.0%
TTGATCCAACCGTTCTTCGG+AGG + chr6.3:65147095-65147114 MS.gene068477:CDS 50.0%
TTTGGTGGAACCACCATCTC+CGG - chr6.3:65146727-65146746 None:intergenic 50.0%
! AGTTGGGATGCCGTTTGTGT+CGG + chr6.3:65147148-65147167 MS.gene068477:CDS 50.0%
! GGCTAGAAGACTTTTGGACG+AGG + chr6.3:65147863-65147882 MS.gene068477:CDS 50.0%
! TTTTACCCGAAGGATCGGAC+CGG + chr6.3:65147201-65147220 MS.gene068477:CDS 50.0%
!! AGATAGCGCCGGTGGAAATT+TGG + chr6.3:65147007-65147026 MS.gene068477:CDS 50.0%
!! GACTTTTGGACGAGGTTGGT+TGG + chr6.3:65147871-65147890 MS.gene068477:CDS 50.0%
AAGCGGGGCTCCAGAATATC+CGG + chr6.3:65147057-65147076 MS.gene068477:CDS 55.0%
ACCATCTCCGGTGACTTCTC+CGG - chr6.3:65146715-65146734 None:intergenic 55.0%
ACTGTTGCCGGAGAAGTCAC+CGG + chr6.3:65146705-65146724 MS.gene068477:CDS 55.0%
AGCGATGCTCTGGTGCAAGT+CGG - chr6.3:65146884-65146903 None:intergenic 55.0%
ATTTCCACCGGCGCTATCTC+CGG - chr6.3:65147006-65147025 None:intergenic 55.0%
CCGTTAACGGGAGTGGTGTT+AGG - chr6.3:65146621-65146640 None:intergenic 55.0%
CGACGACAGCAGAGATTTCC+CGG - chr6.3:65146848-65146867 None:intergenic 55.0%
CGGACCTTCCAAGGAAGAAG+GGG - chr6.3:65146498-65146517 None:intergenic 55.0%
CTGTTTGCCGTAGACTCTGC+CGG + chr6.3:65146826-65146845 MS.gene068477:CDS 55.0%
CTTCGGAGGAGAAGAACGGA+CGG + chr6.3:65147109-65147128 MS.gene068477:CDS 55.0%
CTTCTCCTCCGAAGAACGGT+TGG - chr6.3:65147103-65147122 None:intergenic 55.0%
GCGGACCTTCCAAGGAAGAA+GGG - chr6.3:65146499-65146518 None:intergenic 55.0%
GGAAACACCGTTAACGGGAG+TGG - chr6.3:65146628-65146647 None:intergenic 55.0%
GGCACACCATGTTGCGGTTA+GGG + chr6.3:65147028-65147047 MS.gene068477:CDS 55.0%
GTCCTTGCCACCGACACAAA+CGG - chr6.3:65147161-65147180 None:intergenic 55.0%
GTGACTTCTCCGGCAACAGT+GGG - chr6.3:65146705-65146724 None:intergenic 55.0%
TGCAAGCCCTAACCGCAACA+TGG - chr6.3:65147037-65147056 None:intergenic 55.0%
TGCGGTTAGGGCTTGCAAAG+CGG + chr6.3:65147040-65147059 MS.gene068477:CDS 55.0%
TGGCACACCATGTTGCGGTT+AGG + chr6.3:65147027-65147046 MS.gene068477:CDS 55.0%
TGTCGGTGGCAAGGACTGAT+TGG + chr6.3:65147165-65147184 MS.gene068477:CDS 55.0%
TGTTTGCCGTAGACTCTGCC+GGG + chr6.3:65146827-65146846 MS.gene068477:CDS 55.0%
TTTACCCGAAGGATCGGACC+GGG + chr6.3:65147202-65147221 MS.gene068477:CDS 55.0%
! AACGGGAGTGGTGTTAGGTG+AGG - chr6.3:65146616-65146635 None:intergenic 55.0%
AGCGGGGCTCCAGAATATCC+GGG + chr6.3:65147058-65147077 MS.gene068477:CDS 60.0%
AGGAGAAGAACGGACGGAGG+CGG + chr6.3:65147115-65147134 MS.gene068477:CDS 60.0%
ATGGTCCCGGTCCGATCCTT+CGG - chr6.3:65147210-65147229 None:intergenic 60.0%
CATTAACCGCGCCATGGTCC+CGG - chr6.3:65147223-65147242 None:intergenic 60.0%
CCCGACAAATCCTCCGCGTT+CGG - chr6.3:65147254-65147273 None:intergenic 60.0%
CCGAACGCGGAGGATTTGTC+GGG + chr6.3:65147251-65147270 MS.gene068477:CDS 60.0%
CGCGGACCTTCCAAGGAAGA+AGG - chr6.3:65146500-65146519 None:intergenic 60.0%
CGCGGAGGATTTGTCGGGTT+TGG + chr6.3:65147256-65147275 MS.gene068477:CDS 60.0%
CGGTTAGGGCTTGCAAAGCG+GGG + chr6.3:65147042-65147061 MS.gene068477:CDS 60.0%
GAAAAGTGAAGCCACCGCCG+TGG - chr6.3:65146779-65146798 None:intergenic 60.0%
GCCGAACGCGGAGGATTTGT+CGG + chr6.3:65147250-65147269 MS.gene068477:CDS 60.0%
GCGGTTAGGGCTTGCAAAGC+GGG + chr6.3:65147041-65147060 MS.gene068477:CDS 60.0%
GGAGAAGTCACCGGAGATGG+TGG + chr6.3:65146714-65146733 MS.gene068477:CDS 60.0%
GGTGACTTCTCCGGCAACAG+TGG - chr6.3:65146706-65146725 None:intergenic 60.0%
TGCGACGGATAGCGATGCTC+TGG - chr6.3:65146894-65146913 None:intergenic 60.0%
TTCCTAGCCGGAGATAGCGC+CGG + chr6.3:65146996-65147015 MS.gene068477:CDS 60.0%
! ATGGTAGCGCGGACCTTCCA+AGG - chr6.3:65146507-65146526 None:intergenic 60.0%
! TGCCGTTTGTGTCGGTGGCA+AGG + chr6.3:65147156-65147175 MS.gene068477:CDS 60.0%
! TGGGATGCCGTTTGTGTCGG+TGG + chr6.3:65147151-65147170 MS.gene068477:CDS 60.0%
CGGAGGAGAAGAACGGACGG+AGG + chr6.3:65147112-65147131 MS.gene068477:CDS 65.0%
GAAGGATCGGACCGGGACCA+TGG + chr6.3:65147209-65147228 MS.gene068477:CDS 65.0%
GCCGGAGAAGTCACCGGAGA+TGG + chr6.3:65146711-65146730 MS.gene068477:CDS 65.0%
TGGTCCCGGTCCGATCCTTC+GGG - chr6.3:65147209-65147228 None:intergenic 65.0%
TGTATGTGGGCCGAACGCGG+AGG + chr6.3:65147241-65147260 MS.gene068477:CDS 65.0%
ATCGGACCGGGACCATGGCG+CGG + chr6.3:65147214-65147233 MS.gene068477:CDS 70.0%
CACCGGCGCTATCTCCGGCT+AGG - chr6.3:65147001-65147020 None:intergenic 70.0%
! CTAGCCGGAGATAGCGCCGG+TGG + chr6.3:65146999-65147018 MS.gene068477:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 65146474 65147968 65146474 ID=MS.gene068477
chr6.3 mRNA 65146474 65147968 65146474 ID=MS.gene068477.t1;Parent=MS.gene068477
chr6.3 exon 65146474 65147400 65146474 ID=MS.gene068477.t1.exon1;Parent=MS.gene068477.t1
chr6.3 CDS 65146474 65147400 65146474 ID=cds.MS.gene068477.t1;Parent=MS.gene068477.t1
chr6.3 exon 65147849 65147968 65147849 ID=MS.gene068477.t1.exon2;Parent=MS.gene068477.t1
chr6.3 CDS 65147849 65147968 65147849 ID=cds.MS.gene068477.t1;Parent=MS.gene068477.t1
Gene Sequence

>MS.gene068477

ATGTCGACAAACAAACCCAAACCCCTTCTTCCTTGGAAGGTCCGCGCTACCATTTCACTTCTTACAGTTTTAACAGATGCTTCATGTCGCTCTAACGGCACCGTCAACCGCTGTCTCTTTAACTTCTTCGATCTCAAATCCTCACCTAACACCACTCCCGTTAACGGTGTTTCCACCAAAGACATCACCGTTAACGACGAAAATAACGTTTGGTTCCGTCTCTTTACTCCCACTGTTGCCGGAGAAGTCACCGGAGATGGTGGTTCCACCAAAACAATCTCCCTTCCTGTCATTATTTTCTTCCACGGCGGTGGCTTCACTTTTCTTACTCCTTCCTCTAACCTCTACGATGCTGTTTGCCGTAGACTCTGCCGGGAAATCTCTGCTGTCGTCGTCTCCGTCAACTACCGACTTGCACCAGAGCATCGCTATCCGTCGCAATATGAAGACGGAGAAGAAGTTCTGAGATTTCTTGACGAGAACGCAACAGTTTTACCGGGAAATGCTGACTTGTCAAAATGTTTCCTAGCCGGAGATAGCGCCGGTGGAAATTTGGCACACCATGTTGCGGTTAGGGCTTGCAAAGCGGGGCTCCAGAATATCCGGGTTATCGGGTCAATATTGATCCAACCGTTCTTCGGAGGAGAAGAACGGACGGAGGCGGAGATTCATCTAGTTGGGATGCCGTTTGTGTCGGTGGCAAGGACTGATTGGATGTGGAAAGTGTTTTTACCCGAAGGATCGGACCGGGACCATGGCGCGGTTAATGTATGTGGGCCGAACGCGGAGGATTTGTCGGGTTTGGATTATCCGGATACACTTGTTTTTGTTGGTGGGTTTGATCCTTTGATTGATTGGCAAAAGAGGTATTATGATTGGTTGAAAAAATGTGGGAAAAAAGCTGAGTTGATTGAATATCCAAACATGGTTCATGCTTTTCATGTTTTTCCTGATTTTCCTGAATCTACTCAGTTGATTGTGCAAGTCAAGGATTTTGTCAACAAAGTCTCCAATTCTAAATTATGATTATTGATTATTGTTGTAATGATATTGTTATGATAATAGCAACTGTGTTAAATTATTTGTAGCTAGAACGTACAAGTTGGTATGTTTATGTTTTATTTTAGTTTAATAAATATAATATGAGTGATCTTCATCCTATATGAGTATGTTTTTAGAGTAAACTTTGGAAGTATACATGTCTTGAATTTAATAAATCTGGGTTTGCATAATTTGATATGCCAGATGATGAGCAGTTTGCTTATGGTTTGGAACTGATATGCGTGTTAGAAATTTAGAATAGGTGTTGCTACTACCCAACTTGTGCACAATAGTCAATTACTTGGTTGTGTGGTCCAAAAGTCTACTACAGTAGATGATGACCATTTTGGCTAGAAGACTTTTGGACGAGGTTGGTTGGGAAGATGAATGCTGTAACATTCAAATCAAGAAGAATTTGATTACAAGAACAGTTGGAGATGATAGTATGACTTAG
Protein sequence

>MS.gene068477.t1

MSTNKPKPLLPWKVRATISLLTVLTDASCRSNGTVNRCLFNFFDLKSSPNTTPVNGVSTKDITVNDENNVWFRLFTPTVAGEVTGDGGSTKTISLPVIIFFHGGGFTFLTPSSNLYDAVCRRLCREISAVVVSVNYRLAPEHRYPSQYEDGEEVLRFLDENATVLPGNADLSKCFLAGDSAGGNLAHHVAVRACKAGLQNIRVIGSILIQPFFGGEERTEAEIHLVGMPFVSVARTDWMWKVFLPEGSDRDHGAVNVCGPNAEDLSGLDYPDTLVFVGGFDPLIDWQKRYYDWLKKCGKKAELIEYPNMMMTILARRLLDEVGWEDECCNIQIKKNLITRTVGDDSMT