AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068788


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068788.t1 MTR_2g079630 97.500 280 7 0 6 285 110 389 0.0 552
MS.gene068788.t1 MTR_2g079650 82.562 281 44 2 6 285 109 385 2.07e-155 439
MS.gene068788.t1 MTR_2g013110 47.703 283 129 4 6 283 125 393 8.85e-77 239
MS.gene068788.t1 MTR_7g079680 29.618 314 147 13 5 275 114 396 3.00e-20 90.1
MS.gene068788.t1 MTR_7g079680 31.780 236 130 9 51 275 61 276 2.58e-18 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068788.t1 AT4G27460 53.147 286 119 6 6 283 110 388 7.37e-82 252
MS.gene068788.t1 AT5G53750 48.667 300 123 8 6 283 115 405 2.78e-69 220
MS.gene068788.t1 AT1G65320 26.984 315 154 11 5 275 117 399 2.75e-16 79.0

Find 78 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTGATGATACAGCTTAT+TGG 0.263301 2.3:+18035026 MS.gene068788:intron
CGATGACCTTGCATACTTTC+CGG 0.310170 2.3:-18035664 None:intergenic
AAGGTTGAAGGAAATGAATT+TGG 0.316598 2.3:+18035539 MS.gene068788:CDS
CACAGCAATCAAAGAGCTCT+TGG 0.367612 2.3:-18035736 None:intergenic
GAGGATGATTGGAGTTTAGT+TGG 0.388830 2.3:+18035804 MS.gene068788:CDS
GCCCTCCAGAGGATCTAGTT+AGG 0.395318 2.3:+18035505 MS.gene068788:CDS
CTCTACATTCCTCACATACT+TGG 0.414784 2.3:-18035697 None:intergenic
TGTGGGTTATTGAGGATGAT+TGG 0.421114 2.3:+18035793 MS.gene068788:CDS
GCCATCACAACTTTGTCTGC+AGG 0.430978 2.3:+18035453 MS.gene068788:CDS
CTTCAACAGCAGAAGACGCC+TGG 0.435308 2.3:-18035314 None:intergenic
GATGTGATCAGGTTCTTACT+TGG 0.466946 2.3:+18035201 MS.gene068788:CDS
TAATAGAGTGAATTACTTGT+GGG 0.472091 2.3:+18035776 MS.gene068788:CDS
CTCCATTGATTACTCTTCCC+AGG 0.476252 2.3:+18035296 MS.gene068788:CDS
GGTTGTAAAGGCAAGGTTGA+AGG 0.477069 2.3:+18035527 MS.gene068788:CDS
GCCATTGATCTCATACTTGA+GGG 0.480304 2.3:+18035051 MS.gene068788:CDS
CGAATTGATTGACATGGAAA+GGG 0.483775 2.3:+18034890 None:intergenic
GAATTACTTGTGGGTTATTG+AGG 0.486011 2.3:+18035785 MS.gene068788:CDS
AGGTTCTTACTTGGTTCCAT+TGG 0.493319 2.3:+18035210 MS.gene068788:CDS
GCGAGGAGTTTGACACGGTC+CGG 0.494146 2.3:+18035645 MS.gene068788:CDS
TTCATCTGATGAGGACAGTC+CGG 0.496147 2.3:+18035623 MS.gene068788:CDS
CCGAATTGATTGACATGGAA+AGG 0.498180 2.3:+18034889 None:intergenic
CCCTCCAGAGGATCTAGTTA+GGG 0.498391 2.3:+18035506 MS.gene068788:CDS
AGACGATCGATGGATTGTGC+AGG 0.510025 2.3:-18035249 None:intergenic
GTTGCAATTGTCGACGGTGA+TGG 0.521762 2.3:+18035369 MS.gene068788:CDS
GGATCTAGTTAGGGTTGTAA+AGG 0.524022 2.3:+18035515 MS.gene068788:CDS
CCATCACAACTTTGTCTGCA+GGG 0.524200 2.3:+18035454 MS.gene068788:CDS
ACAGCAATCAAAGAGCTCTT+GGG 0.525945 2.3:-18035735 None:intergenic
TTGTGTGAGGGACTTTGAGA+TGG 0.531894 2.3:-18035337 None:intergenic
CCCCTCAAGTATGAGATCAA+TGG 0.534194 2.3:-18035052 None:intergenic
CTAATAGAGTGAATTACTTG+TGG 0.535417 2.3:+18035775 MS.gene068788:CDS
ACAACCCTAACTAGATCCTC+TGG 0.535562 2.3:-18035510 None:intergenic
TGCCATTGATCTCATACTTG+AGG 0.539564 2.3:+18035050 MS.gene068788:CDS
TTGCAATTGTCGACGGTGAT+GGG 0.542443 2.3:+18035370 MS.gene068788:CDS
GACGGTGATGGGACATTCAT+CGG 0.542508 2.3:+18035381 MS.gene068788:CDS
CCCTGCAGACAAAGTTGTGA+TGG 0.549793 2.3:-18035454 None:intergenic
CATCACAACTTTGTCTGCAG+GGG 0.550293 2.3:+18035455 MS.gene068788:CDS
TAACACAAGAGGATGTGATC+AGG 0.550433 2.3:+18035190 MS.gene068788:CDS
GATAATATTGAGACGATCGA+TGG 0.553025 2.3:-18035259 None:intergenic
GAGTGCACTGAAGCGGCCAA+TGG 0.559548 2.3:-18035226 None:intergenic
ACAACGGTGTTGAGTTCTGT+TGG 0.560590 2.3:+18035166 MS.gene068788:CDS
TGCAAGGTCATCGAGTTACT+CGG 0.561913 2.3:+18035674 MS.gene068788:CDS
CGCCTGGGAAGAGTAATCAA+TGG 0.562410 2.3:-18035298 None:intergenic
ATCACAACATGCAAGTGTGA+AGG 0.567692 2.3:-18035415 None:intergenic
TAGTTAGGGTTGTAAAGGCA+AGG 0.569328 2.3:+18035520 MS.gene068788:CDS
CAACAGAAGTTTGTTGTGTG+AGG 0.577183 2.3:-18035350 None:intergenic
ATCCTCCTCTTCATCTGATG+AGG 0.578040 2.3:+18035614 MS.gene068788:CDS
GACCGTGTCAAACTCCTCGC+CGG 0.578167 2.3:-18035642 None:intergenic
ATCACAACTTTGTCTGCAGG+GGG 0.584206 2.3:+18035456 MS.gene068788:CDS
TCGTCTCAATATTATCAGCT+CGG 0.585428 2.3:+18035266 MS.gene068788:CDS
GTTCTGTTGGTTAACACAAG+AGG 0.588395 2.3:+18035179 MS.gene068788:CDS
TTCAACAGCAGAAGACGCCT+GGG 0.592455 2.3:-18035313 None:intergenic
GTGCAGGGAGTGCACTGAAG+CGG 0.593463 2.3:-18035233 None:intergenic
GTCCTCATCAGATGAAGAGG+AGG 0.596841 2.3:-18035616 None:intergenic
TCACAACATGCAAGTGTGAA+GGG 0.596883 2.3:-18035414 None:intergenic
ACTGTCCTCATCAGATGAAG+AGG 0.604105 2.3:-18035619 None:intergenic
GACGATCGATGGATTGTGCA+GGG 0.604203 2.3:-18035248 None:intergenic
TGCAGACAAAGTTGTGATGG+CGG 0.609998 2.3:-18035451 None:intergenic
AACAGAAGTTTGTTGTGTGA+GGG 0.614439 2.3:-18035349 None:intergenic
CACGGTCCGGAAAGTATGCA+AGG 0.614793 2.3:+18035658 MS.gene068788:CDS
AGGGCGACCACAGTCAATGT+AGG 0.615124 2.3:-18035487 None:intergenic
TGACTGTGGTCGCCCTCCAG+AGG 0.619657 2.3:+18035494 MS.gene068788:CDS
ATGTTGTGATGAGACGGTTG+CGG 0.621625 2.3:+18035428 MS.gene068788:CDS
ACTTCTGTTGCAATTGTCGA+CGG 0.622534 2.3:+18035363 MS.gene068788:CDS
AACAGAACTCAACACCGTTG+TGG 0.629524 2.3:-18035164 None:intergenic
TGATTGACATGGAAAGGGGA+CGG 0.643465 2.3:+18034895 None:intergenic
ACCCTAACTAGATCCTCTGG+AGG 0.646675 2.3:-18035507 None:intergenic
AGAGGAGGATGACATATCAC+CGG 0.649223 2.3:-18035601 None:intergenic
GAATTGATTGACATGGAAAG+GGG 0.650322 2.3:+18034891 None:intergenic
GTTACTCGGCCAAGTATGTG+AGG 0.653188 2.3:+18035688 MS.gene068788:CDS
CATTGATCTCATACTTGAGG+GGG 0.653452 2.3:+18035053 MS.gene068788:CDS
CAAGTATGTGAGGAATGTAG+AGG 0.658949 2.3:+18035698 MS.gene068788:CDS
ACTTGCATGTTGTGATGAGA+CGG 0.665678 2.3:+18035422 MS.gene068788:CDS
CCATTGATCTCATACTTGAG+GGG 0.673886 2.3:+18035052 MS.gene068788:CDS
CCCTAACTAGATCCTCTGGA+GGG 0.680964 2.3:-18035506 None:intergenic
TTGATGGCCTACATTGACTG+TGG 0.683351 2.3:+18035480 MS.gene068788:CDS
GTCCGGCGAGGAGTTTGACA+CGG 0.708960 2.3:+18035640 MS.gene068788:CDS
TTAACAATCAACAGCCACAA+CGG 0.709558 2.3:+18035150 MS.gene068788:CDS
CTGATGAGGACAGTCCGGCG+AGG 0.730013 2.3:+18035628 MS.gene068788:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTTTTCTAATATCTTGA+AGG + chr2.3:18035833-18035852 MS.gene068788:CDS 20.0%
! TAATAGAGTGAATTACTTGT+GGG + chr2.3:18035776-18035795 MS.gene068788:CDS 25.0%
!! AATTTGATGATACAGCTTAT+TGG + chr2.3:18035026-18035045 MS.gene068788:intron 25.0%
AAGGTTGAAGGAAATGAATT+TGG + chr2.3:18035539-18035558 MS.gene068788:CDS 30.0%
ATTTCGCAAACAAAAAAAGG+TGG + chr2.3:18035096-18035115 MS.gene068788:CDS 30.0%
CATCAAATTAACAAGAAGCA+AGG - chr2.3:18035015-18035034 None:intergenic 30.0%
CCAATTTCGCAAACAAAAAA+AGG + chr2.3:18035093-18035112 MS.gene068788:CDS 30.0%
CTAATAGAGTGAATTACTTG+TGG + chr2.3:18035775-18035794 MS.gene068788:CDS 30.0%
!!! CCTTTTTTTGTTTGCGAAAT+TGG - chr2.3:18035096-18035115 None:intergenic 30.0%
CAAAAAAAGGTGGTCTTTCA+AGG + chr2.3:18035106-18035125 MS.gene068788:CDS 35.0%
GATAATATTGAGACGATCGA+TGG - chr2.3:18035262-18035281 None:intergenic 35.0%
TCGTCTCAATATTATCAGCT+CGG + chr2.3:18035266-18035285 MS.gene068788:CDS 35.0%
TTAACAATCAACAGCCACAA+CGG + chr2.3:18035150-18035169 MS.gene068788:CDS 35.0%
! GAATTACTTGTGGGTTATTG+AGG + chr2.3:18035785-18035804 MS.gene068788:CDS 35.0%
!! AACAGAAGTTTGTTGTGTGA+GGG - chr2.3:18035352-18035371 None:intergenic 35.0%
ACAGCAATCAAAGAGCTCTT+GGG - chr2.3:18035738-18035757 None:intergenic 40.0%
ACTTCTGTTGCAATTGTCGA+CGG + chr2.3:18035363-18035382 MS.gene068788:CDS 40.0%
ACTTGCATGTTGTGATGAGA+CGG + chr2.3:18035422-18035441 MS.gene068788:CDS 40.0%
AGGTTCTTACTTGGTTCCAT+TGG + chr2.3:18035210-18035229 MS.gene068788:CDS 40.0%
ATCACAACATGCAAGTGTGA+AGG - chr2.3:18035418-18035437 None:intergenic 40.0%
CAAGTATGTGAGGAATGTAG+AGG + chr2.3:18035698-18035717 MS.gene068788:CDS 40.0%
CATTGATCTCATACTTGAGG+GGG + chr2.3:18035053-18035072 MS.gene068788:CDS 40.0%
CCATTGATCTCATACTTGAG+GGG + chr2.3:18035052-18035071 MS.gene068788:CDS 40.0%
CTCTACATTCCTCACATACT+TGG - chr2.3:18035700-18035719 None:intergenic 40.0%
GAGGATGATTGGAGTTTAGT+TGG + chr2.3:18035804-18035823 MS.gene068788:CDS 40.0%
GATGTGATCAGGTTCTTACT+TGG + chr2.3:18035201-18035220 MS.gene068788:CDS 40.0%
GCCATTGATCTCATACTTGA+GGG + chr2.3:18035051-18035070 MS.gene068788:CDS 40.0%
GGATCTAGTTAGGGTTGTAA+AGG + chr2.3:18035515-18035534 MS.gene068788:CDS 40.0%
TAACACAAGAGGATGTGATC+AGG + chr2.3:18035190-18035209 MS.gene068788:CDS 40.0%
TAGTTAGGGTTGTAAAGGCA+AGG + chr2.3:18035520-18035539 MS.gene068788:CDS 40.0%
TCACAACATGCAAGTGTGAA+GGG - chr2.3:18035417-18035436 None:intergenic 40.0%
TGCCATTGATCTCATACTTG+AGG + chr2.3:18035050-18035069 MS.gene068788:CDS 40.0%
! GTTCTGTTGGTTAACACAAG+AGG + chr2.3:18035179-18035198 MS.gene068788:CDS 40.0%
! TGTGGGTTATTGAGGATGAT+TGG + chr2.3:18035793-18035812 MS.gene068788:CDS 40.0%
!! CAACAGAAGTTTGTTGTGTG+AGG - chr2.3:18035353-18035372 None:intergenic 40.0%
!!! ACGCTTTTGACTTCTTTGAC+CGG + chr2.3:18035582-18035601 MS.gene068788:CDS 40.0%
AACAGAACTCAACACCGTTG+TGG - chr2.3:18035167-18035186 None:intergenic 45.0%
ACAACCCTAACTAGATCCTC+TGG - chr2.3:18035513-18035532 None:intergenic 45.0%
ACTGTCCTCATCAGATGAAG+AGG - chr2.3:18035622-18035641 None:intergenic 45.0%
AGAGGAGGATGACATATCAC+CGG - chr2.3:18035604-18035623 None:intergenic 45.0%
ATCACAACTTTGTCTGCAGG+GGG + chr2.3:18035456-18035475 MS.gene068788:CDS 45.0%
ATCCTCCTCTTCATCTGATG+AGG + chr2.3:18035614-18035633 MS.gene068788:CDS 45.0%
ATGTTGTGATGAGACGGTTG+CGG + chr2.3:18035428-18035447 MS.gene068788:CDS 45.0%
CACAGCAATCAAAGAGCTCT+TGG - chr2.3:18035739-18035758 None:intergenic 45.0%
CATCACAACTTTGTCTGCAG+GGG + chr2.3:18035455-18035474 MS.gene068788:CDS 45.0%
CCATCACAACTTTGTCTGCA+GGG + chr2.3:18035454-18035473 MS.gene068788:CDS 45.0%
CCCCTCAAGTATGAGATCAA+TGG - chr2.3:18035055-18035074 None:intergenic 45.0%
CGATGACCTTGCATACTTTC+CGG - chr2.3:18035667-18035686 None:intergenic 45.0%
GGTTGTAAAGGCAAGGTTGA+AGG + chr2.3:18035527-18035546 MS.gene068788:CDS 45.0%
TGCAAGGTCATCGAGTTACT+CGG + chr2.3:18035674-18035693 MS.gene068788:CDS 45.0%
TGCAGACAAAGTTGTGATGG+CGG - chr2.3:18035454-18035473 None:intergenic 45.0%
TTCATCTGATGAGGACAGTC+CGG + chr2.3:18035623-18035642 MS.gene068788:CDS 45.0%
TTGCAATTGTCGACGGTGAT+GGG + chr2.3:18035370-18035389 MS.gene068788:CDS 45.0%
! ACAACGGTGTTGAGTTCTGT+TGG + chr2.3:18035166-18035185 MS.gene068788:CDS 45.0%
! CTCCATTGATTACTCTTCCC+AGG + chr2.3:18035296-18035315 MS.gene068788:CDS 45.0%
! TTGATGGCCTACATTGACTG+TGG + chr2.3:18035480-18035499 MS.gene068788:CDS 45.0%
! TTGTGTGAGGGACTTTGAGA+TGG - chr2.3:18035340-18035359 None:intergenic 45.0%
ACCCTAACTAGATCCTCTGG+AGG - chr2.3:18035510-18035529 None:intergenic 50.0%
CCCTAACTAGATCCTCTGGA+GGG - chr2.3:18035509-18035528 None:intergenic 50.0%
CCCTCCAGAGGATCTAGTTA+GGG + chr2.3:18035506-18035525 MS.gene068788:CDS 50.0%
CCCTGCAGACAAAGTTGTGA+TGG - chr2.3:18035457-18035476 None:intergenic 50.0%
CGCCTGGGAAGAGTAATCAA+TGG - chr2.3:18035301-18035320 None:intergenic 50.0%
GCCATCACAACTTTGTCTGC+AGG + chr2.3:18035453-18035472 MS.gene068788:CDS 50.0%
GTCCTCATCAGATGAAGAGG+AGG - chr2.3:18035619-18035638 None:intergenic 50.0%
GTTACTCGGCCAAGTATGTG+AGG + chr2.3:18035688-18035707 MS.gene068788:CDS 50.0%
GTTGCAATTGTCGACGGTGA+TGG + chr2.3:18035369-18035388 MS.gene068788:CDS 50.0%
TTCAACAGCAGAAGACGCCT+GGG - chr2.3:18035316-18035335 None:intergenic 50.0%
!! AGACGATCGATGGATTGTGC+AGG - chr2.3:18035252-18035271 None:intergenic 50.0%
!! GACGATCGATGGATTGTGCA+GGG - chr2.3:18035251-18035270 None:intergenic 50.0%
!! GACGGTGATGGGACATTCAT+CGG + chr2.3:18035381-18035400 MS.gene068788:CDS 50.0%
AGGGCGACCACAGTCAATGT+AGG - chr2.3:18035490-18035509 None:intergenic 55.0%
CTTCAACAGCAGAAGACGCC+TGG - chr2.3:18035317-18035336 None:intergenic 55.0%
GCCCTCCAGAGGATCTAGTT+AGG + chr2.3:18035505-18035524 MS.gene068788:CDS 55.0%
TTTGTCTGCAGGGGGCTTGA+TGG + chr2.3:18035464-18035483 MS.gene068788:CDS 55.0%
! CACGGTCCGGAAAGTATGCA+AGG + chr2.3:18035658-18035677 MS.gene068788:CDS 55.0%
! TGAGGGGGCACAGAATCTTG+TGG + chr2.3:18035068-18035087 MS.gene068788:CDS 55.0%
GACCGTGTCAAACTCCTCGC+CGG - chr2.3:18035645-18035664 None:intergenic 60.0%
GAGTGCACTGAAGCGGCCAA+TGG - chr2.3:18035229-18035248 None:intergenic 60.0%
GCGAGGAGTTTGACACGGTC+CGG + chr2.3:18035645-18035664 MS.gene068788:CDS 60.0%
GTCCGGCGAGGAGTTTGACA+CGG + chr2.3:18035640-18035659 MS.gene068788:CDS 60.0%
! GTGCAGGGAGTGCACTGAAG+CGG - chr2.3:18035236-18035255 None:intergenic 60.0%
CTGATGAGGACAGTCCGGCG+AGG + chr2.3:18035628-18035647 MS.gene068788:CDS 65.0%
TGACTGTGGTCGCCCTCCAG+AGG + chr2.3:18035494-18035513 MS.gene068788:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 18034903 18035884 18034903 ID=MS.gene068788
chr2.3 mRNA 18034903 18035884 18034903 ID=MS.gene068788.t1;Parent=MS.gene068788
chr2.3 exon 18034903 18034916 18034903 ID=MS.gene068788.t1.exon1;Parent=MS.gene068788.t1
chr2.3 CDS 18034903 18034916 18034903 ID=cds.MS.gene068788.t1;Parent=MS.gene068788.t1
chr2.3 exon 18035041 18035884 18035041 ID=MS.gene068788.t1.exon2;Parent=MS.gene068788.t1
chr2.3 CDS 18035041 18035884 18035041 ID=cds.MS.gene068788.t1;Parent=MS.gene068788.t1
Gene Sequence

>MS.gene068788

ATGGAAAGGGGACGGTAATTATATATCTGTAATTTCTTGTTGAAGCTTGAAATTCTGTTTTAATGTGTTTGTTTTAATATGATCAAAATTAAGAATTTTAGTTAATTAACCTTGCTTCTTGTTAATTTGATGATACAGCTTATTGGATGCCATTGATCTCATACTTGAGGGGGCACAGAATCTTGTGGTGCCAATTTCGCAAACAAAAAAAGGTGGTCTTTCAAGGAGAAAATTGCAACAGAAGTCATTAACAATCAACAGCCACAACGGTGTTGAGTTCTGTTGGTTAACACAAGAGGATGTGATCAGGTTCTTACTTGGTTCCATTGGCCGCTTCAGTGCACTCCCTGCACAATCCATCGATCGTCTCAATATTATCAGCTCGGATGTGCTCTCCATTGATTACTCTTCCCAGGCGTCTTCTGCTGTTGAAGCCATCTCAAAGTCCCTCACACAACAAACTTCTGTTGCAATTGTCGACGGTGATGGGACATTCATCGGTGAGATATCACCCTTCACACTTGCATGTTGTGATGAGACGGTTGCGGCCGCCATCACAACTTTGTCTGCAGGGGGCTTGATGGCCTACATTGACTGTGGTCGCCCTCCAGAGGATCTAGTTAGGGTTGTAAAGGCAAGGTTGAAGGAAATGAATTTGGAAAAACTGCTACAAGAGTTTACGCTTTTGACTTCTTTGACCGGTGATATGTCATCCTCCTCTTCATCTGATGAGGACAGTCCGGCGAGGAGTTTGACACGGTCCGGAAAGTATGCAAGGTCATCGAGTTACTCGGCCAAGTATGTGAGGAATGTAGAGGCTATAGTTTGTCATCCCAAGAGCTCTTTGATTGCTGTGATGATGCAAGCAATTGCTAATAGAGTGAATTACTTGTGGGTTATTGAGGATGATTGGAGTTTAGTTGGCATTGTTACTTTTTCTAATATCTTGAAGGTGTTCAAAGAGCGTTTAGAAGTTATTTAG

Protein sequence

>MS.gene068788.t1

MERGRLLDAIDLILEGAQNLVVPISQTKKGGLSRRKLQQKSLTINSHNGVEFCWLTQEDVIRFLLGSIGRFSALPAQSIDRLNIISSDVLSIDYSSQASSAVEAISKSLTQQTSVAIVDGDGTFIGEISPFTLACCDETVAAAITTLSAGGLMAYIDCGRPPEDLVRVVKARLKEMNLEKLLQEFTLLTSLTGDMSSSSSSDEDSPARSLTRSGKYARSSSYSAKYVRNVEAIVCHPKSSLIAVMMQAIANRVNYLWVIEDDWSLVGIVTFSNILKVFKERLEVI