AlfalfaGEDB | MS.gene069408

M. sativa cultivar XinJiangDaYe / MS.gene069408

KEGG: K12818
GO: GO:0003674 GO:0003824 GO:0004386 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0042623
PFAM: PF04408.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069408.t1	CAN82460.1	91.2	113	10	0	1	113	924	1036	1.80E-50	208.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069408.t1	Q38953	94.0	100	6	0	1	100	908	1007	1.9e-48	193.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069408.t1	A5AQH3	91.2	113	10	0	1	113	924	1036	1.3e-50	208.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene069408	0.818653	1.75E-52	-1.69E-46
MS.gene049254	MS.gene069408	0.803383	3.58E-49	-1.69E-46
MS.gene051198	MS.gene069408	0.806516	7.92E-50	-1.69E-46
MS.gene051279	MS.gene069408	0.810395	1.18E-50	-1.69E-46
MS.gene051594	MS.gene069408	0.815835	7.54E-52	-1.69E-46
MS.gene051719	MS.gene069408	0.809517	1.82E-50	-1.69E-46
MS.gene051720	MS.gene069408	0.801081	1.07E-48	-1.69E-46
MS.gene052516	MS.gene069408	0.805645	1.21E-49	-1.69E-46
MS.gene052519	MS.gene069408	0.803231	3.85E-49	-1.69E-46
MS.gene052582	MS.gene069408	0.819505	1.12E-52	-1.69E-46
MS.gene052584	MS.gene069408	0.815132	1.08E-51	-1.69E-46
MS.gene052773	MS.gene069408	0.812384	4.36E-51	-1.69E-46
MS.gene053226	MS.gene069408	0.837243	5.83E-57	-1.69E-46
MS.gene053998	MS.gene069408	0.804156	2.47E-49	-1.69E-46
MS.gene054651	MS.gene069408	0.83213	1.13E-55	-1.69E-46
MS.gene054775	MS.gene069408	0.813525	2.45E-51	-1.69E-46
MS.gene054991	MS.gene069408	0.807305	5.39E-50	-1.69E-46
MS.gene055228	MS.gene069408	0.801827	7.49E-49	-1.69E-46
MS.gene055926	MS.gene069408	0.818144	2.28E-52	-1.69E-46
MS.gene056050	MS.gene069408	0.851849	6.79E-61	-1.69E-46
MS.gene056173	MS.gene069408	0.81519	1.05E-51	-1.69E-46
MS.gene056174	MS.gene069408	0.816173	6.34E-52	-1.69E-46
MS.gene056366	MS.gene069408	0.80582	1.11E-49	-1.69E-46
MS.gene05690	MS.gene069408	0.801845	7.43E-49	-1.69E-46
MS.gene057444	MS.gene069408	0.82647	2.67E-54	-1.69E-46
MS.gene057855	MS.gene069408	0.808859	2.52E-50	-1.69E-46
MS.gene058625	MS.gene069408	0.816109	6.55E-52	-1.69E-46
MS.gene058979	MS.gene069408	0.822708	2.05E-53	-1.69E-46
MS.gene059625	MS.gene069408	0.835051	2.10E-56	-1.69E-46
MS.gene060348	MS.gene069408	0.802896	4.51E-49	-1.69E-46
MS.gene060353	MS.gene069408	0.806845	6.75E-50	-1.69E-46
MS.gene060587	MS.gene069408	0.804833	1.79E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069408.t1	MTR_1g023985	98.000	100	2	0	1	100	963	1062	4.93e-62	204
MS.gene069408.t1	MTR_1g114490	94.000	100	6	0	1	100	894	993	8.64e-60	197
MS.gene069408.t1	MTR_1543s0010	54.167	96	44	0	1	96	166	261	9.32e-33	116
MS.gene069408.t1	MTR_3g054400	55.208	96	43	0	1	96	765	860	1.65e-32	119
MS.gene069408.t1	MTR_3g055020	54.167	96	44	0	1	96	142	237	2.01e-32	117
MS.gene069408.t1	MTR_3g052610	48.760	121	53	1	1	112	486	606	8.68e-32	117
MS.gene069408.t1	MTR_2g098440	55.208	96	43	0	1	96	968	1063	1.46e-31	117
MS.gene069408.t1	MTR_1g086640	58.333	96	40	0	1	96	463	558	4.18e-31	115
MS.gene069408.t1	MTR_4g485610	47.934	121	54	1	1	112	425	545	7.89e-31	115
MS.gene069408.t1	MTR_3g023880	52.632	95	43	2	8	100	812	906	1.63e-26	102
MS.gene069408.t1	MTR_6g061840	50.980	102	48	2	1	100	650	751	1.93e-26	102
MS.gene069408.t1	MTR_6g061840	50.980	102	48	2	1	100	650	751	2.05e-26	102
MS.gene069408.t1	MTR_8g041990	56.627	83	34	2	16	96	421	503	1.07e-25	100
MS.gene069408.t1	MTR_5g075310	56.627	83	34	2	16	96	577	659	5.86e-25	98.2
MS.gene069408.t1	MTR_2g015320	39.344	122	64	2	1	112	465	586	3.19e-22	90.5
MS.gene069408.t1	MTR_7g095080	43.158	95	53	1	1	94	483	577	2.81e-19	82.0
MS.gene069408.t1	MTR_8g080240	37.963	108	61	3	1	104	398	503	1.69e-17	77.0
MS.gene069408.t1	MTR_8g080240	37.963	108	61	3	1	104	398	503	1.74e-17	77.0
MS.gene069408.t1	MTR_8g080240	37.963	108	61	3	1	104	398	503	1.78e-17	77.0
MS.gene069408.t1	MTR_4g094992	40.000	95	56	1	1	94	277	371	4.39e-17	75.9
MS.gene069408.t1	MTR_3g054420	46.774	62	33	0	35	96	1	62	8.69e-17	73.9
MS.gene069408.t1	MTR_8g044260	38.462	91	55	1	7	96	718	808	1.45e-14	68.6
MS.gene069408.t1	MTR_1g109170	35.417	96	62	0	1	96	791	886	6.58e-14	66.6
MS.gene069408.t1	MTR_1g109170	35.417	96	62	0	1	96	791	886	7.68e-14	66.6
MS.gene069408.t1	MTR_1g056490	38.776	98	57	2	1	96	689	785	1.03e-13	66.2
MS.gene069408.t1	MTR_8g106630	37.363	91	56	1	7	96	743	833	8.52e-13	63.5
MS.gene069408.t1	MTR_8g045110	34.021	97	62	2	1	96	299	394	1.62e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069408.t1	AT3G26560	94.000	100	6	0	1	100	908	1007	4.07e-60	198
MS.gene069408.t1	AT5G13010	56.250	96	42	0	1	96	955	1050	1.42e-31	117
MS.gene069408.t1	AT2G47250	56.250	96	42	0	1	96	471	566	1.68e-30	114
MS.gene069408.t1	AT3G62310	55.208	96	43	0	1	96	467	562	1.10e-29	111
MS.gene069408.t1	AT1G32490	54.902	102	44	2	1	100	787	888	1.21e-29	111
MS.gene069408.t1	AT1G32490	54.902	102	44	2	1	100	797	898	1.23e-29	111
MS.gene069408.t1	AT4G16680	50.962	104	47	2	1	100	524	627	9.55e-29	108
MS.gene069408.t1	AT2G35340	50.980	102	48	2	1	100	797	898	2.03e-27	105
MS.gene069408.t1	AT2G35340	50.980	102	48	2	1	100	659	760	2.40e-27	105
MS.gene069408.t1	AT5G14900	41.525	118	59	2	1	108	37	154	3.20e-26	99.4
MS.gene069408.t1	AT4G18465	39.344	122	64	2	1	112	460	581	4.92e-21	87.0
MS.gene069408.t1	AT2G01130	35.833	120	65	1	7	114	623	742	9.33e-18	77.8
MS.gene069408.t1	AT2G01130	35.833	120	65	1	7	114	688	807	1.14e-17	77.4
MS.gene069408.t1	AT1G26370	40.860	93	54	1	1	92	475	567	2.20e-17	76.6
MS.gene069408.t1	AT1G27900	35.000	100	60	2	1	95	401	500	3.85e-16	73.2
MS.gene069408.t1	AT1G27900	35.000	100	60	2	1	95	401	500	3.85e-16	73.2
MS.gene069408.t1	AT1G27900	35.000	100	60	2	1	95	401	500	4.02e-16	73.2
MS.gene069408.t1	AT1G48650	35.052	97	62	1	1	96	762	858	4.19e-14	67.4
MS.gene069408.t1	AT1G48650	35.052	97	62	1	1	96	762	858	4.66e-14	67.4
MS.gene069408.t1	AT3G62310	51.724	58	28	0	1	58	467	524	3.57e-13	64.7
MS.gene069408.t1	AT3G62310	53.571	56	26	0	1	56	467	522	3.88e-13	64.7
MS.gene069408.t1	AT5G04895	37.363	91	56	1	7	96	729	819	1.05e-12	63.5
MS.gene069408.t1	AT2G35920	34.694	98	61	2	1	96	629	725	8.47e-12	60.8
MS.gene069408.t1	AT2G35920	34.694	98	61	2	1	96	687	783	1.01e-11	60.5
MS.gene069408.t1	AT2G35920	34.694	98	61	2	1	96	719	815	1.02e-11	60.5

Find 32 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGGGTCTTCTAACCAAAT+TGG	0.277303	1.1:-999355	MS.gene069408:CDS
TGCTTGGGCTTGTTTCTCCC+TGG	0.296594	1.1:+999200	None:intergenic
AGTCTGAATCATGGCAATTA+TGG	0.333399	1.1:+999242	None:intergenic
GCTTTACAGTCTTGGAGCTT+TGG	0.341671	1.1:-999382	MS.gene069408:CDS
AACCAAATTGGGGAGGAAAA+TGG	0.351616	1.1:-999343	MS.gene069408:CDS
GCTTGGGCTTGTTTCTCCCT+GGG	0.368210	1.1:+999201	None:intergenic
GAGGGTCTTCTAACCAAATT+GGG	0.369219	1.1:-999354	MS.gene069408:CDS
GAAAATGGCTGAGTTTCCTT+TGG	0.380268	1.1:-999328	MS.gene069408:CDS
ATGGAGCAGCTTTACAGTCT+TGG	0.431319	1.1:-999390	MS.gene069408:CDS
TGCTTGCTGTTTATGAATCC+TGG	0.431920	1.1:-999128	MS.gene069408:CDS
AAGCCCAAGCAGATCAGAAA+AGG	0.432080	1.1:-999188	MS.gene069408:CDS
TGCTTGCCAGTGTAGACCTT+GGG	0.451872	1.1:-999284	MS.gene069408:CDS
TACACTGGCAAGCAACATCT+TGG	0.456841	1.1:+999293	None:intergenic
TAAGATGGTCACCCTCTGGC+TGG	0.498954	1.1:+999154	None:intergenic
AGCGTAAGATGGTCACCCTC+TGG	0.505396	1.1:+999150	None:intergenic
TATATTGCCAGTCTGAATCA+TGG	0.525241	1.1:+999233	None:intergenic
AGCCCAAGCAGATCAGAAAA+GGG	0.536339	1.1:-999187	MS.gene069408:CDS
TCTTGGAGCTTTGGATGAAG+AGG	0.542469	1.1:-999373	MS.gene069408:CDS
TTGCTTGCCAGTGTAGACCT+TGG	0.555949	1.1:-999285	MS.gene069408:CDS
AAACAGCAAGCAGCGTAAGA+TGG	0.556576	1.1:+999139	None:intergenic
CTTGGAGCTTTGGATGAAGA+GGG	0.570022	1.1:-999372	MS.gene069408:CDS
TTGGACAGCGGCGGATCCAA+AGG	0.573072	1.1:+999312	None:intergenic
AAATCTCATCACTGCACCCA+AGG	0.597096	1.1:+999268	None:intergenic
AGCAACATCTTGGACAGCGG+CGG	0.599358	1.1:+999303	None:intergenic
GCAAGCAACATCTTGGACAG+CGG	0.615292	1.1:+999300	None:intergenic
ATAATTGCCATGATTCAGAC+TGG	0.617453	1.1:-999240	MS.gene069408:CDS
AAGACTGTAAAGCTGCTCCA+TGG	0.623520	1.1:+999392	None:intergenic
GTCTTCTAACCAAATTGGGG+AGG	0.632314	1.1:-999350	MS.gene069408:CDS
ACTGCACCCAAGGTCTACAC+TGG	0.637206	1.1:+999278	None:intergenic
AAGCAGATCAGAAAAGGGCA+AGG	0.651984	1.1:-999182	MS.gene069408:CDS
AGGGTCTTCTAACCAAATTG+GGG	0.707720	1.1:-999353	MS.gene069408:CDS
ACAAAGTTCTCAAAACACCA+AGG	0.730042	1.1:+999085	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTGGAAAATAAAAACTTTTC+TGG	-	chr1.1:999349-999368	MS.gene069408:CDS	25.0%
!!!	CAGAAAAGTTTTTATTTTCC+AGG	+	chr1.1:999351-999370	None:intergenic	25.0%
!	AGACTGGCAATATATTTTAC+AGG	-	chr1.1:999234-999253	MS.gene069408:CDS	30.0%
!	ATAAAAACTTTTCTGGACCT+TGG	-	chr1.1:999356-999375	MS.gene069408:CDS	30.0%
	ACAAAGTTCTCAAAACACCA+AGG	+	chr1.1:999376-999395	None:intergenic	35.0%
	AGTCTGAATCATGGCAATTA+TGG	+	chr1.1:999219-999238	None:intergenic	35.0%
	ATAATTGCCATGATTCAGAC+TGG	-	chr1.1:999218-999237	MS.gene069408:CDS	35.0%
	TATATTGCCAGTCTGAATCA+TGG	+	chr1.1:999228-999247	None:intergenic	35.0%
!	CAATATATTTTACAGGCCCA+GGG	-	chr1.1:999241-999260	MS.gene069408:CDS	35.0%
	AACCAAATTGGGGAGGAAAA+TGG	-	chr1.1:999115-999134	MS.gene069408:CDS	40.0%
	AGAGGGTCTTCTAACCAAAT+TGG	-	chr1.1:999103-999122	MS.gene069408:CDS	40.0%
	AGGGTCTTCTAACCAAATTG+GGG	-	chr1.1:999105-999124	MS.gene069408:CDS	40.0%
	GAAAATGGCTGAGTTTCCTT+TGG	-	chr1.1:999130-999149	MS.gene069408:CDS	40.0%
	GAGGGTCTTCTAACCAAATT+GGG	-	chr1.1:999104-999123	MS.gene069408:CDS	40.0%
!	GCAATATATTTTACAGGCCC+AGG	-	chr1.1:999240-999259	MS.gene069408:CDS	40.0%
!!	TGCTTGCTGTTTATGAATCC+TGG	-	chr1.1:999330-999349	MS.gene069408:CDS	40.0%
	AAACAGCAAGCAGCGTAAGA+TGG	+	chr1.1:999322-999341	None:intergenic	45.0%
	AAATCTCATCACTGCACCCA+AGG	+	chr1.1:999193-999212	None:intergenic	45.0%
	AAGCAGATCAGAAAAGGGCA+AGG	-	chr1.1:999276-999295	MS.gene069408:CDS	45.0%
	AAGCCCAAGCAGATCAGAAA+AGG	-	chr1.1:999270-999289	MS.gene069408:CDS	45.0%
	AGCCCAAGCAGATCAGAAAA+GGG	-	chr1.1:999271-999290	MS.gene069408:CDS	45.0%
	ATGGAGCAGCTTTACAGTCT+TGG	-	chr1.1:999068-999087	MS.gene069408:CDS	45.0%
	GCTTTACAGTCTTGGAGCTT+TGG	-	chr1.1:999076-999095	MS.gene069408:CDS	45.0%
	GTCTTCTAACCAAATTGGGG+AGG	-	chr1.1:999108-999127	MS.gene069408:CDS	45.0%
	TACACTGGCAAGCAACATCT+TGG	+	chr1.1:999168-999187	None:intergenic	45.0%
!	TGCCCTTTTCTGATCTGCTT+GGG	+	chr1.1:999276-999295	None:intergenic	45.0%
!	TTGCCCTTTTCTGATCTGCT+TGG	+	chr1.1:999277-999296	None:intergenic	45.0%
!!	AGCCATTTTCCTCCCCAATT+TGG	+	chr1.1:999120-999139	None:intergenic	45.0%
!!	CTTGGAGCTTTGGATGAAGA+GGG	-	chr1.1:999086-999105	MS.gene069408:CDS	45.0%
!!	TCTTGGAGCTTTGGATGAAG+AGG	-	chr1.1:999085-999104	MS.gene069408:CDS	45.0%
	GCAAGCAACATCTTGGACAG+CGG	+	chr1.1:999161-999180	None:intergenic	50.0%
	TGCTTGCCAGTGTAGACCTT+GGG	-	chr1.1:999174-999193	MS.gene069408:CDS	50.0%
	TTGCTTGCCAGTGTAGACCT+TGG	-	chr1.1:999173-999192	MS.gene069408:CDS	50.0%
!	GCAAGGTTTTTCCAGCCAGA+GGG	-	chr1.1:999293-999312	MS.gene069408:CDS	50.0%
	ACTGCACCCAAGGTCTACAC+TGG	+	chr1.1:999183-999202	None:intergenic	55.0%
	AGCAACATCTTGGACAGCGG+CGG	+	chr1.1:999158-999177	None:intergenic	55.0%
	AGCGTAAGATGGTCACCCTC+TGG	+	chr1.1:999311-999330	None:intergenic	55.0%
	TAAGATGGTCACCCTCTGGC+TGG	+	chr1.1:999307-999326	None:intergenic	55.0%
!	GCTTGGGCTTGTTTCTCCCT+GGG	+	chr1.1:999260-999279	None:intergenic	55.0%
!	GGCAAGGTTTTTCCAGCCAG+AGG	-	chr1.1:999292-999311	MS.gene069408:CDS	55.0%
!	TGCTTGGGCTTGTTTCTCCC+TGG	+	chr1.1:999261-999280	None:intergenic	55.0%
	TTGGACAGCGGCGGATCCAA+AGG	+	chr1.1:999149-999168	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	999068	999412	999068	ID=MS.gene069408
chr1.1	mRNA	999068	999412	999068	ID=MS.gene069408.t1;Parent=MS.gene069408
chr1.1	exon	999068	999412	999068	ID=MS.gene069408.t1.exon1;Parent=MS.gene069408.t1
chr1.1	CDS	999068	999412	999068	ID=cds.MS.gene069408.t1;Parent=MS.gene069408.t1

Gene Sequence	>MS.gene069408 ATGGAGCAGCTTTACAGTCTTGGAGCTTTGGATGAAGAGGGTCTTCTAACCAAATTGGGGAGGAAAATGGCTGAGTTTCCTTTGGATCCGCCGCTGTCCAAGATGTTGCTTGCCAGTGTAGACCTTGGGTGCAGTGATGAGATTTTGACCATAATTGCCATGATTCAGACTGGCAATATATTTTACAGGCCCAGGGAGAAACAAGCCCAAGCAGATCAGAAAAGGGCAAGGTTTTTCCAGCCAGAGGGTGACCATCTTACGCTGCTTGCTGTTTATGAATCCTGGAAAATAAAAACTTTTCTGGACCTTGGTGTTTTGAGAACTTTGTTCAATCTCGATCGTTGA
Protein sequence	>MS.gene069408.t1 MEQLYSLGALDEEGLLTKLGRKMAEFPLDPPLSKMLLASVDLGCSDEILTIIAMIQTGNIFYRPREKQAQADQKRARFFQPEGDHLTLLAVYESWKIKTFLDLGVLRTLFNLDR