AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070005


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070005.t1 MTR_3g115620 96.067 178 7 0 1 178 20 197 2.84e-129 362
MS.gene070005.t1 MTR_1g115315 72.297 148 37 3 1 148 25 168 2.60e-72 219
MS.gene070005.t1 MTR_1g115315 72.297 148 37 3 1 148 25 168 3.07e-72 219
MS.gene070005.t1 MTR_7g023810 47.753 178 67 4 1 175 22 176 5.34e-45 147
MS.gene070005.t1 MTR_2g015000 37.363 91 56 1 33 122 24 114 3.05e-15 73.2
MS.gene070005.t1 MTR_4g084550 44.262 61 34 0 63 123 64 124 2.61e-14 68.2
MS.gene070005.t1 MTR_7g025010 44.444 63 35 0 61 123 45 107 2.42e-13 67.8
MS.gene070005.t1 MTR_1g019130 40.789 76 45 0 63 138 90 165 2.66e-13 66.2
MS.gene070005.t1 MTR_4g088070 36.585 82 52 0 61 142 27 108 4.05e-13 67.0
MS.gene070005.t1 MTR_1g019130 40.789 76 45 0 63 138 90 165 6.03e-13 65.5
MS.gene070005.t1 MTR_8g107210 44.262 61 34 0 63 123 96 156 2.49e-12 64.7
MS.gene070005.t1 MTR_4g088080 45.000 60 33 0 61 120 38 97 4.68e-12 63.5
MS.gene070005.t1 MTR_4g063735 42.623 61 35 0 63 123 25 85 1.74e-11 61.6
MS.gene070005.t1 MTR_7g060630 45.000 60 33 0 63 122 8 67 5.17e-11 59.7
MS.gene070005.t1 MTR_5g081990 38.462 65 40 0 63 127 33 97 8.74e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070005.t1 AT4G35550 54.774 199 74 3 1 184 38 235 3.63e-65 201
MS.gene070005.t1 AT1G20700 48.889 180 79 4 1 178 31 199 2.82e-55 174
MS.gene070005.t1 AT1G20700 48.603 179 79 4 1 177 31 198 1.06e-54 172
MS.gene070005.t1 AT1G20700 48.507 134 58 2 45 178 15 137 9.78e-41 135
MS.gene070005.t1 AT1G20710 46.667 180 86 5 1 178 15 186 4.74e-38 130
MS.gene070005.t1 AT5G45980 46.774 62 33 0 61 122 53 114 1.17e-14 71.2
MS.gene070005.t1 AT2G33880 45.161 62 34 0 61 122 53 114 2.56e-14 70.5
MS.gene070005.t1 AT2G33880 45.161 62 34 0 61 122 53 114 2.87e-14 70.5
MS.gene070005.t1 AT1G46480 47.541 61 32 0 63 123 90 150 1.12e-13 66.6
MS.gene070005.t1 AT1G46480 47.541 61 32 0 63 123 90 150 3.37e-13 66.2
MS.gene070005.t1 AT3G18010 40.845 71 42 0 63 133 76 146 3.84e-13 67.0
MS.gene070005.t1 AT3G11260 36.458 96 56 2 63 155 24 117 1.18e-12 63.5
MS.gene070005.t1 AT2G28610 46.667 60 32 0 63 122 8 67 4.54e-12 63.2
MS.gene070005.t1 AT5G05770 33.333 84 55 1 63 146 29 111 8.69e-12 60.1
MS.gene070005.t1 AT5G59340 43.077 65 37 0 59 123 10 74 1.47e-11 62.0
MS.gene070005.t1 AT2G01500 35.577 104 57 2 49 142 37 140 3.17e-11 60.8

Find 36 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAGACTAATGTTTATAAT+TGG 0.232522 3.1:+91469418 MS.gene070005:CDS
TTGAACGAGCTCTTCTGTTC+TGG 0.269547 3.1:-91469443 None:intergenic
TATGTCTTTGATCTTCTGTT+TGG 0.364760 3.1:-91469364 None:intergenic
TCAACTTCTGTGTCTGCCTC+TGG 0.402663 3.1:-91469507 None:intergenic
GTCTTTGATCTTCTGTTTGG+TGG 0.403902 3.1:-91469361 None:intergenic
CGTTGGAGTCCAACGCTGCC+TGG 0.416225 3.1:-91469286 None:intergenic
CTGGACATAAGATAACTTCC+AGG 0.428363 3.1:+91469268 MS.gene070005:CDS
CTCAACAAGCTGATCAGAAA+TGG 0.436221 3.1:-91467951 None:intergenic
TGAGAGTTTATCACCTCATC+GGG 0.446050 3.1:+91469581 MS.gene070005:CDS
CCACTGATGATGGCTTGCTC+TGG 0.448763 3.1:+91469249 MS.gene070005:CDS
TCTTTGATCTTCTGTTTGGT+GGG 0.460764 3.1:-91469360 None:intergenic
TTGAGAGTTTATCACCTCAT+CGG 0.471970 3.1:+91469580 MS.gene070005:CDS
AGTTATGACCGATGAACAAA+TGG 0.474281 3.1:+91467895 None:intergenic
ACCGTGTTGCCCTAGTTCAA+TGG 0.479896 3.1:-91469388 None:intergenic
TAAAAGTTCCATTTGTTCAT+CGG 0.487682 3.1:-91467903 None:intergenic
TACGCTCAAGTATTTGAAGC+TGG 0.512249 3.1:-91469314 None:intergenic
ATTTGAAGCTGGATAGGCGT+TGG 0.517894 3.1:-91469303 None:intergenic
TTAAGATTAATACCTATGTC+AGG 0.524253 3.1:-91469627 None:intergenic
GTACTGTGATCCACTGATGA+TGG 0.540609 3.1:+91469239 MS.gene070005:CDS
TCAAGTATTTGAAGCTGGAT+AGG 0.541162 3.1:-91469309 None:intergenic
AAGCAATCATCTGTTACTGC+AGG 0.542364 3.1:+91469471 MS.gene070005:CDS
GAATGTTCAGGACTGAGACT+TGG 0.551998 3.1:+91469210 MS.gene070005:intron
TCTATGAATACTCACTAACT+AGG 0.567909 3.1:+91469652 MS.gene070005:CDS
ACCTGCGAGGTCGTGTTGAG+TGG 0.570461 3.1:-91467990 None:intergenic
AAAGACATAACCATTGAACT+AGG 0.570662 3.1:+91469378 MS.gene070005:CDS
ACCACTCAACACGACCTCGC+AGG 0.579613 3.1:+91467989 MS.gene070005:CDS
CCAATTATAAACATTAGTCT+CGG 0.584869 3.1:-91469418 None:intergenic
TTTATCACCTCATCGGGTCA+AGG 0.591799 3.1:+91469587 MS.gene070005:CDS
AGATAACTTCCAGGCAGCGT+TGG 0.599900 3.1:+91469277 MS.gene070005:CDS
AAGACATAACCATTGAACTA+GGG 0.603476 3.1:+91469379 MS.gene070005:CDS
CCAGAGCAAGCCATCATCAG+TGG 0.606100 3.1:-91469249 None:intergenic
TACATATCCTTGACCCGATG+AGG 0.618531 3.1:-91469594 None:intergenic
AGGAAATCATGCAGAGCCAG+AGG 0.631315 3.1:+91469491 MS.gene070005:CDS
ACCATTGAACTAGGGCAACA+CGG 0.634091 3.1:+91469387 MS.gene070005:CDS
TACATTCAGAGTCCTGACAT+AGG 0.642781 3.1:+91469615 MS.gene070005:CDS
ATAGTAGTAGTTTACCTGCG+AGG 0.718032 3.1:-91468003 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTCTCTATTTTTTTA+AGG - chr3.1:91469042-91469061 None:intergenic 10.0%
!! CTGTAATATAACATTTATAT+AGG - chr3.1:91468255-91468274 None:intergenic 15.0%
!! TCTATAAAAAAAAACACTAA+AGG - chr3.1:91468964-91468983 None:intergenic 15.0%
!! AAAAACACTAAAGGTTTAAT+TGG - chr3.1:91468955-91468974 None:intergenic 20.0%
!! ATATAGGCAAATTTGTATAA+GGG - chr3.1:91468239-91468258 None:intergenic 20.0%
!! TATATAGGCAAATTTGTATA+AGG - chr3.1:91468240-91468259 None:intergenic 20.0%
!!! AAAACATTCATTTTTGTAAG+AGG + chr3.1:91468507-91468526 MS.gene070005:intron 20.0%
!!! AATAATGTACGATGATTTTT+CGG - chr3.1:91468906-91468925 None:intergenic 20.0%
!!! GAACAGAAAAATTGATTTTT+TGG - chr3.1:91468829-91468848 None:intergenic 20.0%
!!! TTTTTATAGGTGAATATTAG+TGG + chr3.1:91469075-91469094 MS.gene070005:intron 20.0%
! AAATTAAATGTGGAGAAATG+AGG - chr3.1:91468454-91468473 None:intergenic 25.0%
! ACTAATGGAAACAACTTATT+TGG - chr3.1:91468692-91468711 None:intergenic 25.0%
! AGAACTCACAAAATTAAATG+TGG - chr3.1:91468464-91468483 None:intergenic 25.0%
! CCTATAAAAAAAAACACTCA+AGG - chr3.1:91469065-91469084 None:intergenic 25.0%
! TAAAAGTTCCATTTGTTCAT+CGG - chr3.1:91467906-91467925 None:intergenic 25.0%
! TGTTATATTGTTGATGTCAT+TGG + chr3.1:91468309-91468328 MS.gene070005:intron 25.0%
! TTAAGATTAATACCTATGTC+AGG - chr3.1:91469630-91469649 None:intergenic 25.0%
! TTCAAATAAGTCTATCCAAA+CGG + chr3.1:91468753-91468772 MS.gene070005:intron 25.0%
!! AGAAAGAGAATACATGATTT+TGG + chr3.1:91468188-91468207 MS.gene070005:intron 25.0%
!! CCAATTATAAACATTAGTCT+CGG - chr3.1:91469421-91469440 None:intergenic 25.0%
!!! ACACTTTTTGGAATTACTAA+TGG - chr3.1:91468707-91468726 None:intergenic 25.0%
!!! CCTTGAGTGTTTTTTTTTAT+AGG + chr3.1:91469062-91469081 MS.gene070005:intron 25.0%
AAAGACATAACCATTGAACT+AGG + chr3.1:91469378-91469397 MS.gene070005:CDS 30.0%
AAGACATAACCATTGAACTA+GGG + chr3.1:91469379-91469398 MS.gene070005:CDS 30.0%
CCGAGACTAATGTTTATAAT+TGG + chr3.1:91469418-91469437 MS.gene070005:CDS 30.0%
GACATTGCATAATTTATGCA+GGG + chr3.1:91468407-91468426 MS.gene070005:intron 30.0%
GTCAATGTTATTGAATGTTC+AGG + chr3.1:91469198-91469217 MS.gene070005:intron 30.0%
TATGTCTTTGATCTTCTGTT+TGG - chr3.1:91469367-91469386 None:intergenic 30.0%
TCAAATAAGTCTATCCAAAC+GGG + chr3.1:91468754-91468773 MS.gene070005:intron 30.0%
TCTATGAATACTCACTAACT+AGG + chr3.1:91469652-91469671 MS.gene070005:CDS 30.0%
! TAAACACTTGTGACACTTTT+TGG - chr3.1:91468719-91468738 None:intergenic 30.0%
!! TCACATGTTTTGTTCTCTTT+TGG - chr3.1:91469537-91469556 None:intergenic 30.0%
!!! ATTGATTTTTTGGAATCGAG+AGG - chr3.1:91468819-91468838 None:intergenic 30.0%
AGAGAGCTTATGGAAATAAG+CGG + chr3.1:91468648-91468667 MS.gene070005:intron 35.0%
AGTGTCACAAGTGTTTATGT+CGG + chr3.1:91468722-91468741 MS.gene070005:intron 35.0%
CGACATTGCATAATTTATGC+AGG + chr3.1:91468406-91468425 MS.gene070005:intron 35.0%
CTAGTCCTAAATAAGTAGCT+CGG - chr3.1:91469157-91469176 None:intergenic 35.0%
CTTATTTCCATAAGCTCTCT+AGG - chr3.1:91468648-91468667 None:intergenic 35.0%
TCTTTGATCTTCTGTTTGGT+GGG - chr3.1:91469363-91469382 None:intergenic 35.0%
TTGAGAGTTTATCACCTCAT+CGG + chr3.1:91469580-91469599 MS.gene070005:CDS 35.0%
! AGTGAGTGCAGGATATATTT+TGG - chr3.1:91468781-91468800 None:intergenic 35.0%
! TCAAGTATTTGAAGCTGGAT+AGG - chr3.1:91469312-91469331 None:intergenic 35.0%
!!! TTTTTTGTCTAGTAGCCTAG+TGG - chr3.1:91468489-91468508 None:intergenic 35.0%
AAATTGGTTACAGTGAGTGC+AGG - chr3.1:91468792-91468811 None:intergenic 40.0%
AAGCAATCATCTGTTACTGC+AGG + chr3.1:91469471-91469490 MS.gene070005:CDS 40.0%
ACCAACCGAGCTACTTATTT+AGG + chr3.1:91469149-91469168 MS.gene070005:intron 40.0%
ATAGTAGTAGTTTACCTGCG+AGG - chr3.1:91468006-91468025 None:intergenic 40.0%
CTCAACAAGCTGATCAGAAA+TGG - chr3.1:91467954-91467973 None:intergenic 40.0%
CTGGACATAAGATAACTTCC+AGG + chr3.1:91469268-91469287 MS.gene070005:CDS 40.0%
GACAAGCGCTAAATCAATCA+AGG - chr3.1:91469181-91469200 None:intergenic 40.0%
GGAATCGAGAGGACAAAAAT+TGG - chr3.1:91468808-91468827 None:intergenic 40.0%
GTCTTTGATCTTCTGTTTGG+TGG - chr3.1:91469364-91469383 None:intergenic 40.0%
TACATTCAGAGTCCTGACAT+AGG + chr3.1:91469615-91469634 MS.gene070005:CDS 40.0%
TAGCTATCCTAGAGAGCTTA+TGG + chr3.1:91468638-91468657 MS.gene070005:intron 40.0%
TCCTAAATAAGTAGCTCGGT+TGG - chr3.1:91469153-91469172 None:intergenic 40.0%
TGAGAGTTTATCACCTCATC+GGG + chr3.1:91469581-91469600 MS.gene070005:CDS 40.0%
TGCATAATTTATGCAGGGCT+GGG + chr3.1:91468412-91468431 MS.gene070005:intron 40.0%
TTGCATAATTTATGCAGGGC+TGG + chr3.1:91468411-91468430 MS.gene070005:intron 40.0%
! CGTATTTTCGATGAAGGCAA+TGG + chr3.1:91469333-91469352 MS.gene070005:CDS 40.0%
! CTTGAGCGTATTTTCGATGA+AGG + chr3.1:91469327-91469346 MS.gene070005:CDS 40.0%
! TACGCTCAAGTATTTGAAGC+TGG - chr3.1:91469317-91469336 None:intergenic 40.0%
!! GGATATATTTTGGACCCGTT+TGG - chr3.1:91468771-91468790 None:intergenic 40.0%
!! TTTTGTGAGTTCTAGCCACT+AGG + chr3.1:91468471-91468490 MS.gene070005:intron 40.0%
ACCATTGAACTAGGGCAACA+CGG + chr3.1:91469387-91469406 MS.gene070005:CDS 45.0%
GAATGTTCAGGACTGAGACT+TGG + chr3.1:91469210-91469229 MS.gene070005:intron 45.0%
GTACTGTGATCCACTGATGA+TGG + chr3.1:91469239-91469258 MS.gene070005:CDS 45.0%
TACATATCCTTGACCCGATG+AGG - chr3.1:91469597-91469616 None:intergenic 45.0%
TTGAACGAGCTCTTCTGTTC+TGG - chr3.1:91469446-91469465 None:intergenic 45.0%
TTTATCACCTCATCGGGTCA+AGG + chr3.1:91469587-91469606 MS.gene070005:CDS 45.0%
! ATGTGGAGAAATGAGGTGTC+CGG - chr3.1:91468447-91468466 None:intergenic 45.0%
! ATTTGAAGCTGGATAGGCGT+TGG - chr3.1:91469306-91469325 None:intergenic 45.0%
ACCGTGTTGCCCTAGTTCAA+TGG - chr3.1:91469391-91469410 None:intergenic 50.0%
AGATAACTTCCAGGCAGCGT+TGG + chr3.1:91469277-91469296 MS.gene070005:CDS 50.0%
AGGAAATCATGCAGAGCCAG+AGG + chr3.1:91469491-91469510 MS.gene070005:CDS 50.0%
CTAGTACCTTCAGTGTCCCT+TGG + chr3.1:91469123-91469142 MS.gene070005:intron 50.0%
GCCAACTGTGCTAAGCTCTA+GGG - chr3.1:91468384-91468403 None:intergenic 50.0%
TCAACTTCTGTGTCTGCCTC+TGG - chr3.1:91469510-91469529 None:intergenic 50.0%
TCCCTAGAGCTTAGCACAGT+TGG + chr3.1:91468380-91468399 MS.gene070005:intron 50.0%
TGCCAACTGTGCTAAGCTCT+AGG - chr3.1:91468385-91468404 None:intergenic 50.0%
! TGTGGAGAAATGAGGTGTCC+GGG - chr3.1:91468446-91468465 None:intergenic 50.0%
!! CAAGGGACACTGAAGGTACT+AGG - chr3.1:91469125-91469144 None:intergenic 50.0%
CCAGAGCAAGCCATCATCAG+TGG - chr3.1:91469252-91469271 None:intergenic 55.0%
! GTGGAGAAATGAGGTGTCCG+GGG - chr3.1:91468445-91468464 None:intergenic 55.0%
!! CCACTGATGATGGCTTGCTC+TGG + chr3.1:91469249-91469268 MS.gene070005:CDS 55.0%
!! TAGCTCGGTTGGTGAGCCAA+GGG - chr3.1:91469142-91469161 None:intergenic 55.0%
ACCACTCAACACGACCTCGC+AGG + chr3.1:91467989-91468008 MS.gene070005:CDS 60.0%
ACCTGCGAGGTCGTGTTGAG+TGG - chr3.1:91467993-91468012 None:intergenic 60.0%
GGTGAGCCAAGGGACACTGA+AGG - chr3.1:91469132-91469151 None:intergenic 60.0%
!! GTAGCTCGGTTGGTGAGCCA+AGG - chr3.1:91469143-91469162 None:intergenic 60.0%
CAGGGCTGGGTTTCGAACCC+CGG + chr3.1:91468425-91468444 MS.gene070005:intron 65.0%
CGTTGGAGTCCAACGCTGCC+TGG - chr3.1:91469289-91469308 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 91467899 91469677 91467899 ID=MS.gene070005
chr3.1 mRNA 91467899 91469677 91467899 ID=MS.gene070005.t1;Parent=MS.gene070005
chr3.1 exon 91467899 91468010 91467899 ID=MS.gene070005.t1.exon1;Parent=MS.gene070005.t1
chr3.1 CDS 91467899 91468010 91467899 ID=cds.MS.gene070005.t1;Parent=MS.gene070005.t1
chr3.1 exon 91469220 91469677 91469220 ID=MS.gene070005.t1.exon2;Parent=MS.gene070005.t1
chr3.1 CDS 91469220 91469677 91469220 ID=cds.MS.gene070005.t1;Parent=MS.gene070005.t1
Gene Sequence

>MS.gene070005

ATGACCGATGAACAAATGGAACTTTTAAGACAACAGATATCTGTTTACATCACCATTTCTGATCAGCTTGTTGAGATGCATAAATCTGTTACCACTCAACACGACCTCGCAGGTAAACTACTACTATTTTCTTTCTTGCAATTCTCTATTGTCATCATAGATGTTAGAATTAGTTGCAAGTTTGTTGAGATCAGATAGTATAGTTAGAATTGATATGTATAGAAAACTTATTTTGTAGCTTATATCATCATATAATCGTCTATGAATTAGTTATATATGCTATATATAAAGAAAGAGAATACATGATTTTGGATTCACTTTTTCACATTCAATTATACCCTTATACAAATTTGCCTATATAAATGTTATATTACAGTTTTATTAAAAAAAATAAGAATTTATATTATATTTGTTATATTGTTGATGTCATTGGAAAAAGATGATAATAGTTTGTATACAAAATAAAATTTCATTTTTGTTGTCCCTAGAGCTTAGCACAGTTGGCAGCGACATTGCATAATTTATGCAGGGCTGGGTTTCGAACCCCGGACACCTCATTTCTCCACATTTAATTTTGTGAGTTCTAGCCACTAGGCTACTAGACAAAAAAAACATTCATTTTTGTAAGAGGAAAAAACAAGATTTTATAAAAATTAATTTTCGTAATTTATACACATTAATGCAATAAAAAGAGCAGTAACTGTTAAACTATTTTCATATAATTTATAAGTTGTTTCATTAGCTATCCTAGAGAGCTTATGGAAATAAGCGGAAAACAACTTATGATCTTCCAAATAAGTTGTTTCCATTAGTAATTCCAAAAAGTGTCACAAGTGTTTATGTCGGTAGATAAGTTCAAATAAGTCTATCCAAACGGGTCCAAAATATATCCTGCACTCACTGTAACCAATTTTTGTCCTCTCGATTCCAAAAAATCAATTTTTCTGTTCGATGAGTTCTACAATGTTTCTGAGCAGTAGATTTGCATTGTTTGTATTTGAATACCGAAAAATCATCGTACATTATTGTTAGTTGCTTTACTTAATTATCTTACCAATTAAACCTTTAGTGTTTTTTTTTATAGATTTGCATTGTTTGTCTTGAATACTGAAAGATTATAATACATTATTGTTTATCTCACCTTAAAAAAATAGAGAATTAAACCTTGAGTGTTTTTTTTTATAGGTGAATATTAGTGGTTATGTTCATTTTCATTGTTGAAACCTAGTACCTTCAGTGTCCCTTGGCTCACCAACCGAGCTACTTATTTAGGACTAGACCTTGATTGATTTAGCGCTTGTCAATGTTATTGAATGTTCAGGACTGAGACTTGGTAATTTGTACTGTGATCCACTGATGATGGCTTGCTCTGGACATAAGATAACTTCCAGGCAGCGTTGGACTCCAACGCCTATCCAGCTTCAAATACTTGAGCGTATTTTCGATGAAGGCAATGGCACTCCCACCAAACAGAAGATCAAAGACATAACCATTGAACTAGGGCAACACGGTCAAATATCCGAGACTAATGTTTATAATTGGTTCCAGAACAGAAGAGCTCGTTCAAAGCGCAAGCAATCATCTGTTACTGCAGGAAATCATGCAGAGCCAGAGGCAGACACAGAAGTTGAGTCTCCAAAAGAGAACAAAACATGTGAAGAAAGCGATCTAGTTCAGTTCTTTGAGAGTTTATCACCTCATCGGGTCAAGGATATGTACATTCAGAGTCCTGACATAGGTATTAATCTTAACTTCTATGAATACTCACTAACTAGGTAA

Protein sequence

>MS.gene070005.t1

MTDEQMELLRQQISVYITISDQLVEMHKSVTTQHDLAGLRLGNLYCDPLMMACSGHKITSRQRWTPTPIQLQILERIFDEGNGTPTKQKIKDITIELGQHGQISETNVYNWFQNRRARSKRKQSSVTAGNHAEPEADTEVESPKENKTCEESDLVQFFESLSPHRVKDMYIQSPDIGINLNFYEYSLTR