Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070163.t1 | XP_013470045.1 | 94 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 2.50E-55 | 224.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070163.t1 | Q2V6K1 | 49.1 | 108 | 54 | 1 | 5 | 111 | 7 | 114 | 4.4e-21 | 102.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070163.t1 | A0A072VRL6 | 94.0 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 1.8e-55 | 224.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene070163 | 0.808957 | 2.40E-50 | -1.69E-46 |
| MS.gene049232 | MS.gene070163 | 0.847839 | 8.97E-60 | -1.69E-46 |
| MS.gene049285 | MS.gene070163 | 0.808216 | 3.45E-50 | -1.69E-46 |
| MS.gene050323 | MS.gene070163 | 0.824429 | 8.11E-54 | -1.69E-46 |
| MS.gene050496 | MS.gene070163 | 0.802246 | 6.14E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene070163 | 0.801936 | 7.12E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene070163 | 0.827303 | 1.69E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene070163 | 0.803184 | 3.93E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene070163 | 0.832296 | 1.03E-55 | -1.69E-46 |
| MS.gene051268 | MS.gene070163 | 0.824657 | 7.17E-54 | -1.69E-46 |
| MS.gene051509 | MS.gene070163 | 0.817568 | 3.08E-52 | -1.69E-46 |
| MS.gene052966 | MS.gene070163 | 0.818393 | 2.01E-52 | -1.69E-46 |
| MS.gene053633 | MS.gene070163 | 0.828508 | 8.65E-55 | -1.69E-46 |
| MS.gene054595 | MS.gene070163 | 0.811205 | 7.87E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene070163 | 0.826388 | 2.79E-54 | -1.69E-46 |
| MS.gene055941 | MS.gene070163 | 0.808123 | 3.62E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene070163 | 0.815695 | 8.10E-52 | -1.69E-46 |
| MS.gene056510 | MS.gene070163 | 0.827737 | 1.33E-54 | -1.69E-46 |
| MS.gene056657 | MS.gene070163 | 0.800744 | 1.25E-48 | -1.69E-46 |
| MS.gene059022 | MS.gene070163 | 0.803505 | 3.38E-49 | -1.69E-46 |
| MS.gene05913 | MS.gene070163 | 0.801057 | 1.08E-48 | -1.69E-46 |
| MS.gene059305 | MS.gene070163 | 0.808182 | 3.51E-50 | -1.69E-46 |
| MS.gene059680 | MS.gene070163 | 0.808835 | 2.55E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene070163 | 0.814461 | 1.52E-51 | -1.69E-46 |
| MS.gene060778 | MS.gene070163 | 0.833526 | 5.07E-56 | -1.69E-46 |
| MS.gene061134 | MS.gene070163 | 0.823021 | 1.73E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070163.t1 | MTR_1g107340 | 94.017 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 1.19e-77 | 229 |
| MS.gene070163.t1 | MTR_1g107285 | 92.308 | 117 | 9 | 0 | 1 | 117 | 1 | 117 | 3.19e-72 | 223 |
| MS.gene070163.t1 | MTR_1g107375 | 61.538 | 117 | 37 | 2 | 1 | 117 | 1 | 109 | 1.44e-39 | 137 |
| MS.gene070163.t1 | MTR_1g107365 | 61.404 | 114 | 38 | 1 | 4 | 117 | 41 | 148 | 1.61e-39 | 137 |
| MS.gene070163.t1 | MTR_1g107350 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 3.39e-39 | 136 |
| MS.gene070163.t1 | MTR_1g107315 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 4.10e-37 | 131 |
| MS.gene070163.t1 | MTR_3g049970 | 56.522 | 115 | 48 | 2 | 4 | 117 | 5 | 118 | 3.08e-36 | 128 |
| MS.gene070163.t1 | MTR_8g064880 | 54.206 | 107 | 49 | 0 | 4 | 110 | 5 | 111 | 2.28e-35 | 126 |
| MS.gene070163.t1 | MTR_1g107360 | 51.754 | 114 | 55 | 0 | 4 | 117 | 5 | 118 | 1.25e-34 | 124 |
| MS.gene070163.t1 | MTR_8g064870 | 50.893 | 112 | 53 | 1 | 6 | 117 | 9 | 118 | 3.72e-34 | 123 |
| MS.gene070163.t1 | MTR_1g032490 | 51.261 | 119 | 56 | 1 | 1 | 117 | 1 | 119 | 4.48e-34 | 122 |
| MS.gene070163.t1 | MTR_1g107325 | 64.557 | 79 | 24 | 1 | 1 | 79 | 1 | 75 | 8.25e-26 | 99.4 |
| MS.gene070163.t1 | MTR_5g004940 | 37.615 | 109 | 65 | 1 | 9 | 117 | 18 | 123 | 1.33e-16 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070163.t1 | AT3G21560 | 41.818 | 110 | 63 | 1 | 6 | 114 | 10 | 119 | 1.34e-24 | 97.1 |
| MS.gene070163.t1 | AT4G15480 | 40.708 | 113 | 67 | 0 | 5 | 117 | 16 | 128 | 7.58e-24 | 95.1 |
| MS.gene070163.t1 | AT4G15490 | 44.037 | 109 | 57 | 2 | 5 | 109 | 2 | 110 | 2.84e-21 | 87.8 |
| MS.gene070163.t1 | AT4G15500 | 47.059 | 102 | 49 | 3 | 9 | 109 | 10 | 107 | 1.62e-20 | 85.5 |
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATTTATGATACATGAAAA+TGG | 0.293046 | 1.2:-79901652 | None:intergenic |
| AGAGACAAGGAGGAGCTTAA+TGG | 0.357032 | 1.2:-79901326 | None:intergenic |
| GAGACAAGGAGGAGCTTAAT+GGG | 0.398246 | 1.2:-79901325 | None:intergenic |
| TACGCTGAAATTGAGGTTGC+TGG | 0.419412 | 1.2:+79901580 | MS.gene070163:CDS |
| GCTTTCGAGTTCTTTGATGA+TGG | 0.422696 | 1.2:+79901514 | MS.gene070163:CDS |
| ATAAACCATCTTGTTGGTCT+AGG | 0.470490 | 1.2:+79901367 | MS.gene070163:CDS |
| GACAAAGTAGCAACTCCAAT+TGG | 0.473853 | 1.2:+79901478 | MS.gene070163:CDS |
| AGGAAAATACCTTGCTGCAA+AGG | 0.482858 | 1.2:+79901387 | MS.gene070163:CDS |
| GGAAAATACCTTGCTGCAAA+GGG | 0.485937 | 1.2:+79901388 | MS.gene070163:CDS |
| GCAAAAGTACCATCACCAAT+TGG | 0.487301 | 1.2:-79901493 | None:intergenic |
| TGGTTTATGTGACCTTGTGC+TGG | 0.487980 | 1.2:-79901352 | None:intergenic |
| TCAGAACTACGCTGAAATTG+AGG | 0.500708 | 1.2:+79901573 | MS.gene070163:CDS |
| CGTACCAGATGGTGATGGTT+TGG | 0.507040 | 1.2:+79901537 | MS.gene070163:CDS |
| AAGGCCAAACCATCACCATC+TGG | 0.514473 | 1.2:-79901541 | None:intergenic |
| GGTCACATAAACCATCTTGT+TGG | 0.536025 | 1.2:+79901361 | MS.gene070163:CDS |
| GTAGTTCTGAAGAGCTCTGA+AGG | 0.569331 | 1.2:-79901560 | None:intergenic |
| TTCAGCACAACAGAGACGGC+TGG | 0.599179 | 1.2:+79901424 | MS.gene070163:CDS |
| CAGTGGCACCCTTTGCAGCA+AGG | 0.603274 | 1.2:-79901396 | None:intergenic |
| TTTGATGATGGCGTACCAGA+TGG | 0.614936 | 1.2:+79901526 | MS.gene070163:CDS |
| GTAGCAACTCCAATTGGTGA+TGG | 0.622872 | 1.2:+79901484 | MS.gene070163:CDS |
| TATCTTCAGCACAACAGAGA+CGG | 0.646837 | 1.2:+79901420 | MS.gene070163:CDS |
| CTTGTGCTGGAAAAGAGACA+AGG | 0.654164 | 1.2:-79901339 | None:intergenic |
| GATGGCGTACCAGATGGTGA+TGG | 0.698073 | 1.2:+79901532 | MS.gene070163:CDS |
| GTGCTGGAAAAGAGACAAGG+AGG | 0.705172 | 1.2:-79901336 | None:intergenic |
| TGTTGTGCTGAAGATAACAG+TGG | 0.758469 | 1.2:-79901413 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAACCATCTTGTTGGTCT+AGG | + | chr1.2:79901367-79901386 | MS.gene070163:CDS | 35.0% |
| ! | ATTTTCCTAGACCAACAAGA+TGG | - | chr1.2:79901375-79901394 | None:intergenic | 35.0% |
| AGGAAAATACCTTGCTGCAA+AGG | + | chr1.2:79901387-79901406 | MS.gene070163:CDS | 40.0% | |
| GACAAAGTAGCAACTCCAAT+TGG | + | chr1.2:79901478-79901497 | MS.gene070163:CDS | 40.0% | |
| GGAAAATACCTTGCTGCAAA+GGG | + | chr1.2:79901388-79901407 | MS.gene070163:CDS | 40.0% | |
| GGTCACATAAACCATCTTGT+TGG | + | chr1.2:79901361-79901380 | MS.gene070163:CDS | 40.0% | |
| TATCTTCAGCACAACAGAGA+CGG | + | chr1.2:79901420-79901439 | MS.gene070163:CDS | 40.0% | |
| TCAGAACTACGCTGAAATTG+AGG | + | chr1.2:79901573-79901592 | MS.gene070163:CDS | 40.0% | |
| TGTTGTGCTGAAGATAACAG+TGG | - | chr1.2:79901416-79901435 | None:intergenic | 40.0% | |
| !! | GCAAAAGTACCATCACCAAT+TGG | - | chr1.2:79901496-79901515 | None:intergenic | 40.0% |
| !! | GCTTTCGAGTTCTTTGATGA+TGG | + | chr1.2:79901514-79901533 | MS.gene070163:CDS | 40.0% |
| AGAGACAAGGAGGAGCTTAA+TGG | - | chr1.2:79901329-79901348 | None:intergenic | 45.0% | |
| CTTGTGCTGGAAAAGAGACA+AGG | - | chr1.2:79901342-79901361 | None:intergenic | 45.0% | |
| GAGACAAGGAGGAGCTTAAT+GGG | - | chr1.2:79901328-79901347 | None:intergenic | 45.0% | |
| GTAGCAACTCCAATTGGTGA+TGG | + | chr1.2:79901484-79901503 | MS.gene070163:CDS | 45.0% | |
| TACGCTGAAATTGAGGTTGC+TGG | + | chr1.2:79901580-79901599 | MS.gene070163:CDS | 45.0% | |
| TGGTTTATGTGACCTTGTGC+TGG | - | chr1.2:79901355-79901374 | None:intergenic | 45.0% | |
| ! | CTTGTCTCTTTTCCAGCACA+AGG | + | chr1.2:79901340-79901359 | MS.gene070163:CDS | 45.0% |
| !! | GTAGTTCTGAAGAGCTCTGA+AGG | - | chr1.2:79901563-79901582 | None:intergenic | 45.0% |
| !! | TTTGATGATGGCGTACCAGA+TGG | + | chr1.2:79901526-79901545 | MS.gene070163:CDS | 45.0% |
| AAGGCCAAACCATCACCATC+TGG | - | chr1.2:79901544-79901563 | None:intergenic | 50.0% | |
| GTGCTGGAAAAGAGACAAGG+AGG | - | chr1.2:79901339-79901358 | None:intergenic | 50.0% | |
| !! | CGTACCAGATGGTGATGGTT+TGG | + | chr1.2:79901537-79901556 | MS.gene070163:CDS | 50.0% |
| GATGGCGTACCAGATGGTGA+TGG | + | chr1.2:79901532-79901551 | MS.gene070163:CDS | 55.0% | |
| TTCAGCACAACAGAGACGGC+TGG | + | chr1.2:79901424-79901443 | MS.gene070163:CDS | 55.0% | |
| CAGTGGCACCCTTTGCAGCA+AGG | - | chr1.2:79901399-79901418 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 79901310 | 79901661 | 79901310 | ID=MS.gene070163 |
| chr1.2 | mRNA | 79901310 | 79901661 | 79901310 | ID=MS.gene070163.t1;Parent=MS.gene070163 |
| chr1.2 | exon | 79901310 | 79901661 | 79901310 | ID=MS.gene070163.t1.exon1;Parent=MS.gene070163.t1 |
| chr1.2 | CDS | 79901310 | 79901661 | 79901310 | ID=cds.MS.gene070163.t1;Parent=MS.gene070163.t1 |
| Gene Sequence |
| Protein sequence |
