AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054595


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054595.t1 MTR_4g007190 94.059 101 3 1 1 101 5 102 5.84e-61 190
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054595.t1 AT1G73060 47.059 102 32 3 1 101 1 81 6.47e-17 74.3

Find 12 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACAGATACACCTGATTT+AGG 0.217240 4.3:+37106940 None:intergenic
TTAGGATCGAAACCACGATT+TGG 0.404526 4.3:+37106958 None:intergenic
CGTGGTTTCGATCCTAAATC+AGG 0.444850 4.3:-37106952 MS.gene054595:CDS
GCGAGAACTTCATAAGACTT+AGG 0.454079 4.3:+37106913 None:intergenic
TTAGTAGTAGAAATTGAAGA+AGG 0.484021 4.3:+37107051 None:intergenic
GAAGAAGGTGAAGAAGATAG+TGG 0.505084 4.3:+37107066 None:intergenic
GATAGTGGAGAAAGATGTGA+AGG 0.532078 4.3:+37107081 None:intergenic
GGAAGAAAAGTGAGGTTGTG+AGG 0.536168 4.3:+37107102 None:intergenic
AATGCTACTATCCATGGCTA+TGG 0.594263 4.3:-37107139 None:intergenic
AGATGACATAGCCATAGCCA+TGG 0.618729 4.3:+37107128 None:intergenic
TCTTCATCAGAGCCAAATCG+TGG 0.636063 4.3:-37106970 MS.gene054595:CDS
GATGTGAAGGAAGAAAAGTG+AGG 0.687118 4.3:+37107094 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTAGTAGTAGAAATTGAAGA+AGG + chr4.3:37106922-37106941 None:intergenic 25.0%
TAAACAGATACACCTGATTT+AGG + chr4.3:37107033-37107052 None:intergenic 30.0%
! TGATGAAGAAGCTTCTTTTT+CGG + chr4.3:37106989-37107008 None:intergenic 30.0%
! CTCAATTTTGCTCTTCAAGA+TGG - chr4.3:37107099-37107118 MS.gene054595:CDS 35.0%
GAAGAAGGTGAAGAAGATAG+TGG + chr4.3:37106907-37106926 None:intergenic 40.0%
GATGTGAAGGAAGAAAAGTG+AGG + chr4.3:37106879-37106898 None:intergenic 40.0%
GCGAGAACTTCATAAGACTT+AGG + chr4.3:37107060-37107079 None:intergenic 40.0%
TTAGGATCGAAACCACGATT+TGG + chr4.3:37107015-37107034 None:intergenic 40.0%
!! GATAGTGGAGAAAGATGTGA+AGG + chr4.3:37106892-37106911 None:intergenic 40.0%
AGATGACATAGCCATAGCCA+TGG + chr4.3:37106845-37106864 None:intergenic 45.0%
CGTGGTTTCGATCCTAAATC+AGG - chr4.3:37107018-37107037 MS.gene054595:CDS 45.0%
GATGACATAGCCATAGCCAT+GGG + chr4.3:37106844-37106863 None:intergenic 45.0%
GGAAGAAAAGTGAGGTTGTG+AGG + chr4.3:37106871-37106890 None:intergenic 45.0%
TCTTCATCAGAGCCAAATCG+TGG - chr4.3:37107000-37107019 MS.gene054595:CDS 45.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 37106844 37107148 37106844 ID=MS.gene054595
chr4.3 mRNA 37106844 37107148 37106844 ID=MS.gene054595.t1;Parent=MS.gene054595
chr4.3 exon 37106844 37107148 37106844 ID=MS.gene054595.t1.exon1;Parent=MS.gene054595.t1
chr4.3 CDS 37106844 37107148 37106844 ID=cds.MS.gene054595.t1;Parent=MS.gene054595.t1
Gene Sequence

>MS.gene054595

ATGGCTATGGCTATGTCATCTCTACCTCACAACCTCACTTTTCTTCCTTCACATCTTTCTCCACTATCTTCTTCACCTTCTTCAATTTCTACTACTAAATCTCTTCGAAACACTTCTTTATTCAAAATCAAATGCATTAAAACCGAAAAAGAAGCTTCTTCATCAGAGCCAAATCGTGGTTTCGATCCTAAATCAGGTGTATCTGTTTACAAACCTAAGTCTTATGAAGTTCTCGCTACTGATGCTGCTAATTCACTCAATTTTGCTCTTCAAGATGGAAAACTGAGACTCGAAATCGATTTCCC

Protein sequence

>MS.gene054595.t1

MAMAMSSLPHNLTFLPSHLSPLSSSPSSISTTKSLRNTSLFKIKCIKTEKEASSSEPNRGFDPKSGVSVYKPKSYEVLATDAANSLNFALQDGKLRLEIDF