Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071751.t1 | PNX65784.1 | 85.3 | 75 | 7 | 1 | 1 | 75 | 1 | 71 | 4.20E-24 | 120.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071751.t1 | A0A392T8J3 | 88.0 | 75 | 7 | 1 | 1 | 75 | 1 | 73 | 3.6e-25 | 123.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene071751 | 0.832814 | 7.62E-56 | -1.69E-46 |
| MS.gene049411 | MS.gene071751 | 0.82463 | 7.27E-54 | -1.69E-46 |
| MS.gene050277 | MS.gene071751 | 0.828762 | 7.51E-55 | -1.69E-46 |
| MS.gene050437 | MS.gene071751 | 0.818255 | 2.16E-52 | -1.69E-46 |
| MS.gene050689 | MS.gene071751 | 0.839527 | 1.50E-57 | -1.69E-46 |
| MS.gene050752 | MS.gene071751 | 0.833827 | 4.26E-56 | -1.69E-46 |
| MS.gene050838 | MS.gene071751 | 0.828551 | 8.44E-55 | -1.69E-46 |
| MS.gene050955 | MS.gene071751 | 0.839074 | 1.97E-57 | -1.69E-46 |
| MS.gene051003 | MS.gene071751 | 0.82293 | 1.82E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene071751 | 0.822808 | 1.94E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene071751 | 0.813209 | 2.87E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene071751 | 0.806562 | 7.74E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene071751 | 0.831524 | 1.59E-55 | -1.69E-46 |
| MS.gene055162 | MS.gene071751 | 0.807326 | 5.34E-50 | -1.69E-46 |
| MS.gene055979 | MS.gene071751 | 0.804635 | 1.96E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene071751 | 0.827502 | 1.51E-54 | -1.69E-46 |
| MS.gene057705 | MS.gene071751 | 0.843077 | 1.75E-58 | -1.69E-46 |
| MS.gene058055 | MS.gene071751 | 0.810883 | 9.24E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene071751 | 0.802428 | 5.64E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene071751 | 0.805278 | 1.44E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071751.t1 | MTR_2g019240 | 84.416 | 77 | 3 | 2 | 1 | 75 | 1 | 70 | 4.76e-38 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071751.t1 | AT2G34585 | 76.190 | 42 | 10 | 0 | 1 | 42 | 1 | 42 | 9.45e-19 | 73.2 |
Find 19 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGTTAGGGGCGGGGTTGAG+TGG | 0.375759 | 2.3:-67434312 | None:intergenic |
| TCGTCGTCGGGATCGTAATC+AGG | 0.386527 | 2.3:-67434270 | None:intergenic |
| TTGACGACCTTGTTCGAGGC+AGG | 0.394231 | 2.3:-67434226 | None:intergenic |
| GCAGTGGTAGCAGTGATTCT+AGG | 0.395537 | 2.3:+67434174 | MS.gene071751:CDS |
| GCAGCGGTGGTGGAGGCGAC+GGG | 0.399580 | 2.3:-67434336 | None:intergenic |
| AGCAGCGGTGGTGGAGGCGA+CGG | 0.425781 | 2.3:-67434337 | None:intergenic |
| AGGATTGAGATTGCGATCAA+TGG | 0.439079 | 2.3:-67434250 | None:intergenic |
| ACTGCGTTGTCTTCGTCGTC+GGG | 0.450375 | 2.3:-67434282 | None:intergenic |
| AACTGCGTTGTCTTCGTCGT+CGG | 0.458028 | 2.3:-67434283 | None:intergenic |
| AGTGAATACCACTTATGAAA+CGG | 0.507725 | 2.3:-67434364 | None:intergenic |
| CAGCGGTGGTGGAGGCGACG+GGG | 0.518735 | 2.3:-67434335 | None:intergenic |
| TGGCTTGACGACCTTGTTCG+AGG | 0.522648 | 2.3:-67434230 | None:intergenic |
| GGGATCATGGGAGCAATCAC+CGG | 0.545095 | 2.3:+67434141 | None:intergenic |
| TGACGACCTTGTTCGAGGCA+GGG | 0.551454 | 2.3:-67434225 | None:intergenic |
| TGTAAACCCTGCCTCGAACA+AGG | 0.577165 | 2.3:+67434219 | MS.gene071751:CDS |
| TACCACTGCTATCACTGCAC+CGG | 0.586478 | 2.3:-67434160 | None:intergenic |
| AGCGGTGGTGGAGGCGACGG+GGG | 0.608456 | 2.3:-67434334 | None:intergenic |
| TGGTAGCAGTGATTCTAGGT+TGG | 0.618694 | 2.3:+67434178 | MS.gene071751:CDS |
| CACCGGTGCAGTGATAGCAG+TGG | 0.664884 | 2.3:+67434158 | MS.gene071751:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGAAACGGTTTTAGCAG+CGG | - | chr2.3:67434355-67434374 | None:intergenic | 35.0% |
| AGGATTGAGATTGCGATCAA+TGG | - | chr2.3:67434253-67434272 | None:intergenic | 40.0% | |
| TGGTAGCAGTGATTCTAGGT+TGG | + | chr2.3:67434178-67434197 | MS.gene071751:CDS | 45.0% | |
| AACTGCGTTGTCTTCGTCGT+CGG | - | chr2.3:67434286-67434305 | None:intergenic | 50.0% | |
| GCAGTGGTAGCAGTGATTCT+AGG | + | chr2.3:67434174-67434193 | MS.gene071751:CDS | 50.0% | |
| TACCACTGCTATCACTGCAC+CGG | - | chr2.3:67434163-67434182 | None:intergenic | 50.0% | |
| TGTAAACCCTGCCTCGAACA+AGG | + | chr2.3:67434219-67434238 | MS.gene071751:CDS | 50.0% | |
| !! | TGAAACGGTTTTAGCAGCGG+TGG | - | chr2.3:67434352-67434371 | None:intergenic | 50.0% |
| ACTGCGTTGTCTTCGTCGTC+GGG | - | chr2.3:67434285-67434304 | None:intergenic | 55.0% | |
| TCGTCGTCGGGATCGTAATC+AGG | - | chr2.3:67434273-67434292 | None:intergenic | 55.0% | |
| TGACGACCTTGTTCGAGGCA+GGG | - | chr2.3:67434228-67434247 | None:intergenic | 55.0% | |
| TGGCTTGACGACCTTGTTCG+AGG | - | chr2.3:67434233-67434252 | None:intergenic | 55.0% | |
| TTGACGACCTTGTTCGAGGC+AGG | - | chr2.3:67434229-67434248 | None:intergenic | 55.0% | |
| !! | AACGGTTTTAGCAGCGGTGG+TGG | - | chr2.3:67434349-67434368 | None:intergenic | 55.0% |
| CACCGGTGCAGTGATAGCAG+TGG | + | chr2.3:67434158-67434177 | MS.gene071751:CDS | 60.0% | |
| !! | GGTTTTAGCAGCGGTGGTGG+AGG | - | chr2.3:67434346-67434365 | None:intergenic | 60.0% |
| GCGTTAGGGGCGGGGTTGAG+TGG | - | chr2.3:67434315-67434334 | None:intergenic | 70.0% | |
| !! | AGCAGCGGTGGTGGAGGCGA+CGG | - | chr2.3:67434340-67434359 | None:intergenic | 70.0% |
| !! | TGGAGGCGACGGGGGCGTTA+GGG | - | chr2.3:67434329-67434348 | None:intergenic | 70.0% |
| !! | AGCGGTGGTGGAGGCGACGG+GGG | - | chr2.3:67434337-67434356 | None:intergenic | 75.0% |
| !! | CAGCGGTGGTGGAGGCGACG+GGG | - | chr2.3:67434338-67434357 | None:intergenic | 75.0% |
| !! | GCAGCGGTGGTGGAGGCGAC+GGG | - | chr2.3:67434339-67434358 | None:intergenic | 75.0% |
| !! | GGAGGCGACGGGGGCGTTAG+GGG | - | chr2.3:67434328-67434347 | None:intergenic | 75.0% |
| !! | GTGGAGGCGACGGGGGCGTT+AGG | - | chr2.3:67434330-67434349 | None:intergenic | 75.0% |
| !!! | CGACGGGGGCGTTAGGGGCG+GGG | - | chr2.3:67434323-67434342 | None:intergenic | 80.0% |
| !!! | GCGACGGGGGCGTTAGGGGC+GGG | - | chr2.3:67434324-67434343 | None:intergenic | 80.0% |
| !!! | GGCGACGGGGGCGTTAGGGG+CGG | - | chr2.3:67434325-67434344 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 67434147 | 67434374 | 67434147 | ID=MS.gene071751 |
| chr2.3 | mRNA | 67434147 | 67434374 | 67434147 | ID=MS.gene071751.t1;Parent=MS.gene071751 |
| chr2.3 | exon | 67434147 | 67434374 | 67434147 | ID=MS.gene071751.t1.exon1;Parent=MS.gene071751.t1 |
| chr2.3 | CDS | 67434147 | 67434374 | 67434147 | ID=cds.MS.gene071751.t1;Parent=MS.gene071751.t1 |
| Gene Sequence |
| Protein sequence |
