AlfalfaGEDB | MS.gene072013

M. sativa cultivar XinJiangDaYe / MS.gene072013

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072013.t1	XP_003593045.1	97.3	147	4	0	1	147	1	147	2.20E-53	218.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072013.t1	A2Q3U1	97.3	147	4	0	1	147	1	147	1.6e-53	218.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049341	MS.gene072013	0.819143	1.36E-52	-1.69E-46
MS.gene049350	MS.gene072013	0.821408	4.10E-53	-1.69E-46
MS.gene049887	MS.gene072013	0.816707	4.81E-52	-1.69E-46
MS.gene050807	MS.gene072013	0.852632	4.06E-61	-1.69E-46
MS.gene050808	MS.gene072013	0.800791	1.22E-48	-1.69E-46
MS.gene051687	MS.gene072013	0.821749	3.42E-53	-1.69E-46
MS.gene052133	MS.gene072013	0.841766	3.89E-58	-1.69E-46
MS.gene052302	MS.gene072013	0.832898	7.27E-56	-1.69E-46
MS.gene052376	MS.gene072013	0.831792	1.37E-55	-1.69E-46
MS.gene052856	MS.gene072013	0.83902	2.03E-57	-1.69E-46
MS.gene052858	MS.gene072013	0.842717	2.18E-58	-1.69E-46
MS.gene052860	MS.gene072013	0.8329	7.26E-56	-1.69E-46
MS.gene052947	MS.gene072013	0.814216	1.72E-51	-1.69E-46
MS.gene053563	MS.gene072013	0.808692	2.73E-50	-1.69E-46
MS.gene053581	MS.gene072013	0.811914	5.52E-51	-1.69E-46
MS.gene055465	MS.gene072013	0.81388	2.05E-51	-1.69E-46
MS.gene055466	MS.gene072013	0.806041	9.97E-50	-1.69E-46
MS.gene055468	MS.gene072013	0.818679	1.73E-52	-1.69E-46
MS.gene055469	MS.gene072013	0.80308	4.13E-49	-1.69E-46
MS.gene056830	MS.gene072013	-0.802676	5.01E-49	-1.69E-46
MS.gene058852	MS.gene072013	0.853045	3.10E-61	-1.69E-46
MS.gene058906	MS.gene072013	0.807001	6.26E-50	-1.69E-46
MS.gene059171	MS.gene072013	0.807065	6.06E-50	-1.69E-46
MS.gene06001	MS.gene072013	0.824758	6.79E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072013.t1	MTR_2g007190	97.959	147	3	0	1	147	1	147	4.86e-101	286

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072013.t1	AT1G60870	59.184	147	60	0	1	147	1	147	1.58e-33	115

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTGAGAAAGCAAGAAATA+TGG	0.282094	2.4:+75594577	MS.gene072013:CDS
GCTTGGAAAGGAATTTCTTC+TGG	0.317736	2.4:+75594686	MS.gene072013:CDS
AGCAGCTGCCCATGCTTTGT+AGG	0.349595	2.4:-75594393	None:intergenic
AAGAAATATGGGAAACTTGA+TGG	0.389367	2.4:+75594589	MS.gene072013:CDS
GTGCCATGGATGAATTTAGA+AGG	0.394252	2.4:+75594498	MS.gene072013:CDS
GGAATATCTCGACTCGGCTA+TGG	0.422650	2.4:+75594472	MS.gene072013:CDS
GAGGATTTCAAGAGATGAAG+TGG	0.462068	2.4:+75594538	MS.gene072013:CDS
GCTGAGAAAGCAAGAAATAT+GGG	0.463308	2.4:+75594578	MS.gene072013:CDS
ATAAGATCTTCGATCGTCGA+TGG	0.506443	2.4:-75594350	None:intergenic
GAAAGGAATTTCTTCTGGCA+AGG	0.513277	2.4:+75594691	MS.gene072013:CDS
AGATAGAATCCTACAAAGCA+TGG	0.513381	2.4:+75594384	MS.gene072013:CDS
AAAGGAAGGTGAACTGAGAA+AGG	0.516993	2.4:-75594295	None:intergenic
CTGCTTCTATGAAATCTGCT+TGG	0.529588	2.4:+75594669	MS.gene072013:CDS
GGTTTGAAGAAGAACTTGAG+AGG	0.547329	2.4:+75594519	MS.gene072013:CDS
AAACCTTCTAAATTCATCCA+TGG	0.552800	2.4:-75594501	None:intergenic
AAAGTATATATGTATCAAGA+TGG	0.576287	2.4:-75594719	None:intergenic
CTCGGCTATGGAAAGTGCCA+TGG	0.577831	2.4:+75594484	MS.gene072013:CDS
TCTATGAAATCTGCTTGGAA+AGG	0.578134	2.4:+75594674	MS.gene072013:CDS
TAAAGATTGATCTGAAAGGA+AGG	0.581920	2.4:-75594309	None:intergenic
CGTGTAAAGATTGATCTGAA+AGG	0.585025	2.4:-75594313	None:intergenic
AGCCGAGTCGAGATATTCCT+CGG	0.604035	2.4:-75594468	None:intergenic
TGAACTTGAGCAAGAAAGAG+AGG	0.624850	2.4:+75594415	MS.gene072013:CDS
AGCCGAGGAATATCTCGACT+CGG	0.631008	2.4:+75594466	MS.gene072013:CDS
GATAGAATCCTACAAAGCAT+GGG	0.638623	2.4:+75594385	MS.gene072013:CDS
AGATGCAATGCAAGAAGCCG+AGG	0.640190	2.4:+75594451	MS.gene072013:CDS
ACTTGAGCAAGAAAGAGAGG+TGG	0.641638	2.4:+75594418	MS.gene072013:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACCTTCTAAATTCATCCA+TGG	-	chr2.4:75594504-75594523	None:intergenic	30.0%
	AAGAAATATGGGAAACTTGA+TGG	+	chr2.4:75594589-75594608	MS.gene072013:CDS	30.0%
	TAAAGATTGATCTGAAAGGA+AGG	-	chr2.4:75594312-75594331	None:intergenic	30.0%
	AGATAGAATCCTACAAAGCA+TGG	+	chr2.4:75594384-75594403	MS.gene072013:CDS	35.0%
	AGCTGAGAAAGCAAGAAATA+TGG	+	chr2.4:75594577-75594596	MS.gene072013:CDS	35.0%
	CGTGTAAAGATTGATCTGAA+AGG	-	chr2.4:75594316-75594335	None:intergenic	35.0%
	GATAGAATCCTACAAAGCAT+GGG	+	chr2.4:75594385-75594404	MS.gene072013:CDS	35.0%
	GCTGAGAAAGCAAGAAATAT+GGG	+	chr2.4:75594578-75594597	MS.gene072013:CDS	35.0%
	TCTATGAAATCTGCTTGGAA+AGG	+	chr2.4:75594674-75594693	MS.gene072013:CDS	35.0%
	AAAGGAAGGTGAACTGAGAA+AGG	-	chr2.4:75594298-75594317	None:intergenic	40.0%
	ATAAGATCTTCGATCGTCGA+TGG	-	chr2.4:75594353-75594372	None:intergenic	40.0%
	CTGCTTCTATGAAATCTGCT+TGG	+	chr2.4:75594669-75594688	MS.gene072013:CDS	40.0%
	GAAAGGAATTTCTTCTGGCA+AGG	+	chr2.4:75594691-75594710	MS.gene072013:CDS	40.0%
	GAGGATTTCAAGAGATGAAG+TGG	+	chr2.4:75594538-75594557	MS.gene072013:CDS	40.0%
	GCTTGGAAAGGAATTTCTTC+TGG	+	chr2.4:75594686-75594705	MS.gene072013:CDS	40.0%
	GGTTTGAAGAAGAACTTGAG+AGG	+	chr2.4:75594519-75594538	MS.gene072013:CDS	40.0%
	GTGCCATGGATGAATTTAGA+AGG	+	chr2.4:75594498-75594517	MS.gene072013:CDS	40.0%
	TGAACTTGAGCAAGAAAGAG+AGG	+	chr2.4:75594415-75594434	MS.gene072013:CDS	40.0%
	ACTTGAGCAAGAAAGAGAGG+TGG	+	chr2.4:75594418-75594437	MS.gene072013:CDS	45.0%
	AGATGCAATGCAAGAAGCCG+AGG	+	chr2.4:75594451-75594470	MS.gene072013:CDS	50.0%
	AGCCGAGGAATATCTCGACT+CGG	+	chr2.4:75594466-75594485	MS.gene072013:CDS	50.0%
	AGCCGAGTCGAGATATTCCT+CGG	-	chr2.4:75594471-75594490	None:intergenic	50.0%
!!	GGAATATCTCGACTCGGCTA+TGG	+	chr2.4:75594472-75594491	MS.gene072013:CDS	50.0%
	AGCAGCTGCCCATGCTTTGT+AGG	-	chr2.4:75594396-75594415	None:intergenic	55.0%
!!	CTCGGCTATGGAAAGTGCCA+TGG	+	chr2.4:75594484-75594503	MS.gene072013:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	75594284	75594727	75594284	ID=MS.gene072013
chr2.4	mRNA	75594284	75594727	75594284	ID=MS.gene072013.t1;Parent=MS.gene072013
chr2.4	exon	75594284	75594727	75594284	ID=MS.gene072013.t1.exon1;Parent=MS.gene072013.t1
chr2.4	CDS	75594284	75594727	75594284	ID=cds.MS.gene072013.t1;Parent=MS.gene072013.t1

Gene Sequence

>MS.gene072013

ATGGAAGCTCTCCTTTCTCAGTTCACCTTCCTTTCAGATCAATCTTTACACGACAAGAGCTTCGATCCATCGACGATCGAAGATCTTATGAAACTATTTGAGATAGAATCCTACAAAGCATGGGCAGCTGCTGAACTTGAGCAAGAAAGAGAGGTGGAAGAAGCAGAAGATGCAATGCAAGAAGCCGAGGAATATCTCGACTCGGCTATGGAAAGTGCCATGGATGAATTTAGAAGGTTTGAAGAAGAACTTGAGAGGATTTCAAGAGATGAAGTGGAGAGTTTAGTTCAAACAGCTGAGAAAGCAAGAAATATGGGAAACTTGATGGAAAAAACAGCTTCTATTGCTTCTAAGAAATATATTGAAGCTGCACTTAATTCAGCTACTGCTTCTATGAAATCTGCTTGGAAAGGAATTTCTTCTGGCAAGGTTCATCCATCTTGA

Protein sequence

>MS.gene072013.t1

MEALLSQFTFLSDQSLHDKSFDPSTIEDLMKLFEIESYKAWAAAELEQEREVEEAEDAMQEAEEYLDSAMESAMDEFRRFEEELERISRDEVESLVQTAEKARNMGNLMEKTASIASKKYIEAALNSATASMKSAWKGISSGKVHPS