Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072915.t1 | AES79525.2 | 85.5 | 55 | 7 | 1 | 1 | 54 | 56 | 110 | 1.50E-15 | 91.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072915.t1 | G7KSA4 | 85.5 | 55 | 7 | 1 | 1 | 54 | 56 | 110 | 1.1e-15 | 91.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene072915 | 0.810162 | 1.32E-50 | -1.69E-46 |
| MS.gene049262 | MS.gene072915 | 0.805897 | 1.07E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene072915 | 0.810045 | 1.40E-50 | -1.69E-46 |
| MS.gene050043 | MS.gene072915 | 0.802818 | 4.68E-49 | -1.69E-46 |
| MS.gene051181 | MS.gene072915 | 0.803886 | 2.81E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene072915 | 0.809724 | 1.64E-50 | -1.69E-46 |
| MS.gene051575 | MS.gene072915 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene072915 | 0.800648 | 1.31E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene072915 | 0.812149 | 4.90E-51 | -1.69E-46 |
| MS.gene054991 | MS.gene072915 | 0.818035 | 2.42E-52 | -1.69E-46 |
| MS.gene055056 | MS.gene072915 | 0.805848 | 1.09E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene072915 | 0.80188 | 7.31E-49 | -1.69E-46 |
| MS.gene05925 | MS.gene072915 | 0.801613 | 8.29E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene072915 | 0.828362 | 9.38E-55 | -1.69E-46 |
| MS.gene060257 | MS.gene072915 | 0.801576 | 8.44E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072915.t1 | MTR_7g069410 | 85.455 | 55 | 7 | 1 | 1 | 54 | 56 | 110 | 1.18e-25 | 90.5 |
| MS.gene072915.t1 | MTR_4g130640 | 80.000 | 55 | 10 | 1 | 1 | 54 | 99 | 153 | 4.33e-23 | 85.1 |
| MS.gene072915.t1 | MTR_5g079130 | 46.667 | 75 | 11 | 1 | 1 | 46 | 1 | 75 | 1.54e-14 | 62.0 |
| MS.gene072915.t1 | MTR_6g023775 | 50.000 | 72 | 7 | 1 | 4 | 46 | 90 | 161 | 3.96e-14 | 62.4 |
| MS.gene072915.t1 | MTR_7g031710 | 70.270 | 37 | 11 | 0 | 17 | 53 | 42 | 78 | 7.27e-12 | 54.7 |
| MS.gene072915.t1 | MTR_6g465000 | 68.571 | 35 | 11 | 0 | 18 | 52 | 35 | 69 | 8.62e-11 | 51.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAATAAAATGGATCCAAAA+AGG | 0.253008 | 3.4:-93436206 | None:intergenic |
| TGAGTTATCTTTAGATGAAC+CGG | 0.418024 | 3.4:-93436130 | MS.gene072915:CDS |
| GCTACAATGTTCTTTGATGA+TGG | 0.418641 | 3.4:-93436099 | MS.gene072915:CDS |
| TCCAACATTCACTACTTAAG+AGG | 0.452504 | 3.4:+93436041 | None:intergenic |
| AACATTGTAGCCTCCAACTC+CGG | 0.456994 | 3.4:+93436111 | None:intergenic |
| ATCTTTAGATGAACCGGAGT+TGG | 0.478188 | 3.4:-93436124 | MS.gene072915:CDS |
| TTTAGATGAACCGGAGTTGG+AGG | 0.500804 | 3.4:-93436121 | MS.gene072915:CDS |
| ATGTTCTTTGATGATGGAAA+CGG | 0.513119 | 3.4:-93436093 | MS.gene072915:CDS |
| GTGCATACTAATGTTGGAGT+TGG | 0.524300 | 3.4:-93436165 | MS.gene072915:CDS |
| GTTTATGTGCATACTAATGT+TGG | 0.532305 | 3.4:-93436171 | MS.gene072915:CDS |
| AGAGGAAACACGGATTGTAT+CGG | 0.568490 | 3.4:+93436059 | None:intergenic |
| TTCTTTGATGATGGAAACGG+AGG | 0.597380 | 3.4:-93436090 | MS.gene072915:CDS |
| TGATGATGGAAACGGAGGAG+AGG | 0.621097 | 3.4:-93436085 | MS.gene072915:CDS |
| TCACTACTTAAGAGGAAACA+CGG | 0.653134 | 3.4:+93436049 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTTATGTGCATACTAATGT+TGG | - | chr3.4:93436083-93436102 | MS.gene072915:CDS | 30.0% | |
| TGAGTTATCTTTAGATGAAC+CGG | - | chr3.4:93436124-93436143 | MS.gene072915:CDS | 30.0% | |
| !! | ATGTTCTTTGATGATGGAAA+CGG | - | chr3.4:93436161-93436180 | MS.gene072915:CDS | 30.0% |
| GCTACAATGTTCTTTGATGA+TGG | - | chr3.4:93436155-93436174 | MS.gene072915:CDS | 35.0% | |
| ATCTTTAGATGAACCGGAGT+TGG | - | chr3.4:93436130-93436149 | MS.gene072915:CDS | 40.0% | |
| GTGCATACTAATGTTGGAGT+TGG | - | chr3.4:93436089-93436108 | MS.gene072915:CDS | 40.0% | |
| TCCAAAAAGGGCTGAAGATT+TGG | - | chr3.4:93436061-93436080 | MS.gene072915:CDS | 40.0% | |
| ! | ACCAAATCTTCAGCCCTTTT+TGG | + | chr3.4:93436065-93436084 | None:intergenic | 40.0% |
| ! | AGAGGAAACACGGATTGTAT+CGG | + | chr3.4:93436198-93436217 | None:intergenic | 40.0% |
| !! | TTCTTTGATGATGGAAACGG+AGG | - | chr3.4:93436164-93436183 | MS.gene072915:CDS | 40.0% |
| AACATTGTAGCCTCCAACTC+CGG | + | chr3.4:93436146-93436165 | None:intergenic | 45.0% | |
| TTTAGATGAACCGGAGTTGG+AGG | - | chr3.4:93436133-93436152 | MS.gene072915:CDS | 45.0% | |
| !! | TGATGATGGAAACGGAGGAG+AGG | - | chr3.4:93436169-93436188 | MS.gene072915:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 93436056 | 93436220 | 93436056 | ID=MS.gene072915 |
| chr3.4 | mRNA | 93436056 | 93436220 | 93436056 | ID=MS.gene072915.t1;Parent=MS.gene072915 |
| chr3.4 | exon | 93436056 | 93436220 | 93436056 | ID=MS.gene072915.t1.exon1;Parent=MS.gene072915.t1 |
| chr3.4 | CDS | 93436056 | 93436220 | 93436056 | ID=cds.MS.gene072915.t1;Parent=MS.gene072915.t1 |
| Gene Sequence |
| Protein sequence |
