Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074027.t1 | XP_003613674.2 | 98.1 | 476 | 9 | 0 | 1 | 476 | 1 | 476 | 8.00E-262 | 912.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074027.t1 | Q8L736 | 59.9 | 486 | 166 | 10 | 1 | 476 | 1 | 467 | 4.3e-139 | 496.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074027.t1 | G7KCH5 | 98.1 | 476 | 9 | 0 | 1 | 476 | 1 | 476 | 5.8e-262 | 912.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055886 | MS.gene074027 | 0.821861 | 3.22E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene074027 | MS.gene06516 | PPI |
| MS.gene074027 | MS.gene055886 | PPI |
| MS.gene074027 | MS.gene013419 | PPI |
| MS.gene059347 | MS.gene074027 | PPI |
| MS.gene074027 | MS.gene34711 | PPI |
| MS.gene074027 | MS.gene83931 | PPI |
| MS.gene074027 | MS.gene25443 | PPI |
| MS.gene074027 | MS.gene91909 | PPI |
| MS.gene074027 | MS.gene032830 | PPI |
| MS.gene074027 | MS.gene049391 | PPI |
| MS.gene074027 | MS.gene63251 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074027.t1 | MTR_5g039390 | 98.319 | 476 | 8 | 0 | 1 | 476 | 1 | 476 | 0.0 | 972 |
| MS.gene074027.t1 | MTR_8g468030 | 73.870 | 509 | 88 | 7 | 1 | 476 | 1 | 497 | 0.0 | 734 |
| MS.gene074027.t1 | MTR_4g108280 | 63.760 | 367 | 128 | 2 | 112 | 476 | 17 | 380 | 5.42e-173 | 491 |
| MS.gene074027.t1 | MTR_4g022260 | 60.656 | 366 | 143 | 1 | 111 | 476 | 53 | 417 | 6.10e-161 | 462 |
| MS.gene074027.t1 | MTR_0345s0010 | 61.190 | 353 | 136 | 1 | 124 | 476 | 84 | 435 | 7.17e-161 | 462 |
| MS.gene074027.t1 | MTR_0105s0130 | 61.190 | 353 | 136 | 1 | 124 | 476 | 84 | 435 | 2.02e-160 | 461 |
| MS.gene074027.t1 | MTR_0105s0170 | 60.907 | 353 | 137 | 1 | 124 | 476 | 97 | 448 | 9.58e-160 | 460 |
| MS.gene074027.t1 | MTR_0105s0020 | 60.340 | 353 | 139 | 1 | 124 | 476 | 84 | 435 | 3.15e-159 | 458 |
| MS.gene074027.t1 | MTR_0105s0040 | 60.907 | 353 | 137 | 1 | 124 | 476 | 182 | 533 | 4.02e-159 | 461 |
| MS.gene074027.t1 | MTR_0496s0010 | 59.563 | 366 | 141 | 3 | 111 | 476 | 53 | 411 | 6.89e-158 | 454 |
| MS.gene074027.t1 | MTR_1330s0010 | 60.807 | 347 | 135 | 1 | 124 | 470 | 97 | 442 | 6.18e-156 | 450 |
| MS.gene074027.t1 | MTR_4g022400 | 59.322 | 354 | 143 | 1 | 123 | 476 | 62 | 414 | 1.04e-151 | 438 |
| MS.gene074027.t1 | MTR_2g094093 | 50.282 | 354 | 163 | 4 | 128 | 476 | 19 | 364 | 2.87e-115 | 343 |
| MS.gene074027.t1 | MTR_7g029290 | 38.596 | 342 | 193 | 9 | 142 | 474 | 68 | 401 | 2.06e-66 | 219 |
| MS.gene074027.t1 | MTR_6g023805 | 37.534 | 365 | 190 | 9 | 130 | 474 | 57 | 403 | 2.40e-63 | 211 |
| MS.gene074027.t1 | MTR_0105s0060 | 56.044 | 91 | 40 | 0 | 386 | 476 | 15 | 105 | 6.31e-35 | 126 |
| MS.gene074027.t1 | MTR_3g085570 | 28.980 | 245 | 149 | 7 | 125 | 352 | 31 | 267 | 9.75e-22 | 97.1 |
| MS.gene074027.t1 | MTR_8g080370 | 26.446 | 242 | 159 | 5 | 125 | 352 | 47 | 283 | 2.16e-16 | 81.3 |
| MS.gene074027.t1 | MTR_3g073640 | 23.851 | 348 | 191 | 11 | 129 | 408 | 43 | 384 | 1.34e-15 | 79.3 |
| MS.gene074027.t1 | MTR_8g085600 | 26.408 | 284 | 181 | 9 | 129 | 401 | 77 | 343 | 2.76e-15 | 78.2 |
| MS.gene074027.t1 | MTR_4g114110 | 25.243 | 309 | 201 | 11 | 105 | 401 | 57 | 347 | 5.28e-15 | 77.4 |
| MS.gene074027.t1 | MTR_2g008440 | 23.810 | 357 | 191 | 12 | 125 | 408 | 39 | 387 | 1.47e-13 | 73.2 |
| MS.gene074027.t1 | MTR_4g011900 | 24.800 | 250 | 162 | 5 | 129 | 352 | 5 | 254 | 2.00e-13 | 72.0 |
| MS.gene074027.t1 | MTR_7g095220 | 22.511 | 231 | 161 | 4 | 138 | 351 | 13 | 242 | 5.72e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074027.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene074027.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene074027.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene074027.t1 | AT1G14330 | 59.290 | 479 | 154 | 8 | 1 | 476 | 1 | 441 | 0.0 | 550 |
| MS.gene074027.t1 | AT1G26930 | 68.378 | 370 | 114 | 1 | 110 | 476 | 52 | 421 | 0.0 | 531 |
| MS.gene074027.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene074027.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene074027.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene074027.t1 | AT1G26930 | 57.838 | 370 | 101 | 2 | 110 | 476 | 52 | 369 | 4.88e-149 | 430 |
| MS.gene074027.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene074027.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene074027.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene074027.t1 | AT3G27150 | 37.673 | 361 | 198 | 10 | 130 | 474 | 71 | 420 | 9.55e-67 | 220 |
| MS.gene074027.t1 | AT3G27150 | 37.673 | 361 | 198 | 10 | 130 | 474 | 71 | 420 | 9.55e-67 | 220 |
| MS.gene074027.t1 | AT5G40680 | 35.119 | 336 | 206 | 4 | 107 | 437 | 43 | 371 | 4.92e-61 | 205 |
| MS.gene074027.t1 | AT1G16250 | 26.061 | 330 | 208 | 10 | 128 | 433 | 7 | 324 | 2.97e-24 | 104 |
| MS.gene074027.t1 | AT1G16250 | 26.061 | 330 | 208 | 10 | 128 | 433 | 7 | 324 | 2.97e-24 | 104 |
| MS.gene074027.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene074027.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene074027.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene074027.t1 | AT1G15670 | 25.648 | 347 | 209 | 14 | 129 | 445 | 3 | 330 | 7.49e-17 | 82.4 |
| MS.gene074027.t1 | AT1G55270 | 26.496 | 234 | 157 | 5 | 129 | 352 | 78 | 306 | 1.71e-16 | 82.0 |
| MS.gene074027.t1 | AT3G61350 | 23.054 | 334 | 215 | 12 | 115 | 432 | 6 | 313 | 2.20e-14 | 74.7 |
| MS.gene074027.t1 | AT3G61350 | 23.054 | 334 | 215 | 12 | 115 | 432 | 6 | 313 | 2.20e-14 | 74.7 |
| MS.gene074027.t1 | AT1G80440 | 24.540 | 326 | 208 | 11 | 129 | 432 | 3 | 312 | 7.52e-14 | 73.2 |
| MS.gene074027.t1 | AT1G22040 | 25.862 | 348 | 183 | 11 | 129 | 408 | 43 | 383 | 7.56e-14 | 73.9 |
| MS.gene074027.t1 | AT1G27420 | 24.206 | 252 | 150 | 7 | 124 | 350 | 5 | 240 | 4.60e-12 | 67.8 |
| MS.gene074027.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 4 | 214 | 2.97e-11 | 65.1 |
| MS.gene074027.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 11 | 221 | 3.01e-11 | 65.1 |
| MS.gene074027.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 11 | 221 | 3.01e-11 | 65.1 |
Find 119 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGGAGATTCGTCGGATTC+TGG | 0.248232 | 5.1:+29689202 | MS.gene074027:CDS |
| TGCATCCACCTTTGGCGAAT+TGG | 0.251940 | 5.1:-29689433 | None:intergenic |
| ATGGTTGGGGTCTTGCATTT+AGG | 0.276947 | 5.1:+29690088 | MS.gene074027:CDS |
| TGGTTGGGGTCTTGCATTTA+GGG | 0.285142 | 5.1:+29690089 | MS.gene074027:CDS |
| GGAAATATGCTCATTGTTAT+TGG | 0.306561 | 5.1:+29690117 | MS.gene074027:CDS |
| CATGAAGTACATGCTGGATT+TGG | 0.313702 | 5.1:+29688919 | MS.gene074027:CDS |
| TTCATCGACATCGCTCGTTC+TGG | 0.316008 | 5.1:-29690066 | None:intergenic |
| GGAACTGAACTTCTTGTGTT+TGG | 0.321969 | 5.1:+29689517 | MS.gene074027:CDS |
| GTTTCATTGAACTCAATTCT+TGG | 0.330175 | 5.1:+29690163 | MS.gene074027:CDS |
| AGGAATTTCTGTCCATGTTC+TGG | 0.338733 | 5.1:-29689875 | None:intergenic |
| ATCTATAAACCTCGCCGCTT+CGG | 0.350189 | 5.1:-29689336 | None:intergenic |
| ATGCTGACATGGAAGTTAAA+AGG | 0.357590 | 5.1:+29690001 | MS.gene074027:CDS |
| GCATCCACCTTTGGCGAATT+GGG | 0.360483 | 5.1:-29689432 | None:intergenic |
| GAACTGAACTTCTTGTGTTT+GGG | 0.361067 | 5.1:+29689518 | MS.gene074027:CDS |
| CGCTGTTCAAGGTCTGATTA+TGG | 0.366547 | 5.1:+29689274 | MS.gene074027:CDS |
| TGTGTCTCAGTCGCTGTTCA+AGG | 0.376165 | 5.1:+29689263 | MS.gene074027:CDS |
| TCTCGTGTGCCCTCCTTGAA+TGG | 0.387707 | 5.1:+29689398 | MS.gene074027:CDS |
| TTTCATTGAACTCAATTCTT+GGG | 0.403492 | 5.1:+29690164 | MS.gene074027:CDS |
| TTGACAAATTCATGGTCCTC+TGG | 0.419803 | 5.1:+29689580 | MS.gene074027:CDS |
| TCGGCAAATGCATCCACCTT+TGG | 0.425014 | 5.1:-29689441 | None:intergenic |
| CGTTTATAATTGTGCTGTGA+TGG | 0.426012 | 5.1:+29690245 | MS.gene074027:CDS |
| ACAAACATTCAAGGGCTCAT+TGG | 0.427663 | 5.1:-29689104 | None:intergenic |
| CTCGTGTGCCCTCCTTGAAT+GGG | 0.432187 | 5.1:+29689399 | MS.gene074027:CDS |
| TTCCTCGGTTTATTCATGCT+CGG | 0.436739 | 5.1:-29689748 | None:intergenic |
| GGCGGTGCCTCGGTTGCAGC+AGG | 0.445232 | 5.1:-29689937 | None:intergenic |
| AAACCGAGGAAGATGTGTTC+TGG | 0.445291 | 5.1:+29689760 | MS.gene074027:CDS |
| ATTAGCAGGTGGTTGTGATT+CGG | 0.446104 | 5.1:+29689663 | MS.gene074027:CDS |
| TCCTCGGTTTATTCATGCTC+GGG | 0.446460 | 5.1:-29689747 | None:intergenic |
| GGATTCTGTTCTTTCTCAAA+CGG | 0.450478 | 5.1:+29689072 | MS.gene074027:CDS |
| AGCGATGTCGATGAATGGTT+GGG | 0.451823 | 5.1:+29690074 | MS.gene074027:CDS |
| AAACGGTATCATGGAACATT+GGG | 0.454394 | 5.1:+29689369 | MS.gene074027:CDS |
| ACAATTATAAACGAAGTTAC+CGG | 0.457419 | 5.1:-29690235 | None:intergenic |
| GGTCGTACGCTTCCCATTCA+AGG | 0.463650 | 5.1:-29689411 | None:intergenic |
| GATGTGTTCTGGTGTATTCA+TGG | 0.466838 | 5.1:+29689771 | MS.gene074027:CDS |
| AGAACCCCTACTACTTCGTC+CGG | 0.468311 | 5.1:-29689908 | None:intergenic |
| TGAGAAAGAACAGAATCCTT+AGG | 0.471913 | 5.1:-29689067 | None:intergenic |
| AACTCCTTGACAAACATTCA+AGG | 0.473412 | 5.1:-29689113 | None:intergenic |
| AATGCTCCAAGATGTTTGTT+CGG | 0.474359 | 5.1:+29689613 | MS.gene074027:CDS |
| CGCCGCTTCGGATTATTTCA+CGG | 0.477509 | 5.1:-29689324 | None:intergenic |
| GAGCGATGTCGATGAATGGT+TGG | 0.478296 | 5.1:+29690073 | MS.gene074027:CDS |
| ATCTTGTCACATAGTTGAAA+CGG | 0.478670 | 5.1:+29689024 | MS.gene074027:CDS |
| TATTTCGTTGCAACATCCTA+AGG | 0.480105 | 5.1:+29689051 | MS.gene074027:CDS |
| GTCGTTGCTGTGTTGTTTCG+AGG | 0.487767 | 5.1:+29688855 | MS.gene074027:CDS |
| TGAGATGCCTGCTGCAACCG+AGG | 0.492241 | 5.1:+29689930 | MS.gene074027:CDS |
| GAAGCGGCGAGGTTTATAGA+TGG | 0.493954 | 5.1:+29689338 | MS.gene074027:CDS |
| GGTGAGATTGCGATATTAGC+AGG | 0.494746 | 5.1:+29689649 | MS.gene074027:CDS |
| CTGAACTTCTTGTGTTTGGG+AGG | 0.494791 | 5.1:+29689521 | MS.gene074027:CDS |
| AAACCTTCACCATGAGTCCT+AGG | 0.500082 | 5.1:-29690144 | None:intergenic |
| GATAAAGAATCTTTAGCTGT+AGG | 0.501866 | 5.1:+29689496 | MS.gene074027:CDS |
| GTCTTACATGAAGTACATGC+TGG | 0.509303 | 5.1:+29688913 | MS.gene074027:CDS |
| GGCAATGAGAGGCGGTGCCT+CGG | 0.509621 | 5.1:-29689947 | None:intergenic |
| AACATCTTAGTACACTTCCT+AGG | 0.510935 | 5.1:-29688998 | None:intergenic |
| AATATGCTCATTGTTATTGG+TGG | 0.514731 | 5.1:+29690120 | MS.gene074027:CDS |
| TCTGATGAGGAGCAGCATGC+TGG | 0.518838 | 5.1:+29689184 | MS.gene074027:CDS |
| GTGGATGCATTTGCCGAGGA+TGG | 0.521975 | 5.1:+29689447 | MS.gene074027:CDS |
| GCAGAGCCGAACAAACATCT+TGG | 0.522841 | 5.1:-29689619 | None:intergenic |
| CTGAGGATGAACATCATCCT+AGG | 0.526740 | 5.1:+29688981 | MS.gene074027:CDS |
| TATAGATGGAGGAGACTAAA+CGG | 0.529185 | 5.1:+29689352 | MS.gene074027:CDS |
| GTTTATAATTGTGCTGTGAT+GGG | 0.533513 | 5.1:+29690246 | MS.gene074027:CDS |
| TAAACGGTATCATGGAACAT+TGG | 0.533894 | 5.1:+29689368 | MS.gene074027:CDS |
| GAGGAGACTAAACGGTATCA+TGG | 0.537620 | 5.1:+29689360 | MS.gene074027:CDS |
| GAACGAGCGATGTCGATGAA+TGG | 0.544102 | 5.1:+29690069 | MS.gene074027:CDS |
| TAGGAAGTGTACTAAGATGT+TGG | 0.544939 | 5.1:+29689000 | MS.gene074027:CDS |
| GAACCCCTACTACTTCGTCC+GGG | 0.545858 | 5.1:-29689907 | None:intergenic |
| ACACCAGAACACATCTTCCT+CGG | 0.549483 | 5.1:-29689763 | None:intergenic |
| GACACACGATTGAGCTGTCT+CGG | 0.549997 | 5.1:-29689246 | None:intergenic |
| TTGTTCGGCTCTGCAAGCCT+TGG | 0.557116 | 5.1:+29689628 | MS.gene074027:CDS |
| TGAACTTCTTGTGTTTGGGA+GGG | 0.558574 | 5.1:+29689522 | MS.gene074027:CDS |
| CATTGCGGAGGTCCTTCACT+AGG | 0.564491 | 5.1:-29690189 | None:intergenic |
| CTACTAGCTCGAAAACGATC+CGG | 0.564520 | 5.1:+29690216 | MS.gene074027:CDS |
| GAAATTCCTGACATGTCCCC+CGG | 0.564869 | 5.1:+29689889 | MS.gene074027:CDS |
| CTTGTGAAACAGAGAACGAT+TGG | 0.567058 | 5.1:+29688891 | MS.gene074027:CDS |
| GAGCTGTCTCGGTTCATCCT+CGG | 0.567515 | 5.1:-29689235 | None:intergenic |
| AGCAGGTGGTTGTGATTCGG+AGG | 0.569771 | 5.1:+29689666 | MS.gene074027:CDS |
| CAATCGTTTGAAGCCATCCT+CGG | 0.574471 | 5.1:-29689460 | None:intergenic |
| TGTTCTGGTGTATTCATGGA+TGG | 0.587848 | 5.1:+29689775 | MS.gene074027:CDS |
| AGAAAACTGTGGATTACTGT+TGG | 0.588100 | 5.1:+29690036 | MS.gene074027:CDS |
| TGAAATAATCCGAAGCGGCG+AGG | 0.588835 | 5.1:+29689327 | MS.gene074027:CDS |
| ACGACCCAATTCGCCAAAGG+TGG | 0.595170 | 5.1:+29689428 | MS.gene074027:CDS |
| AATTCTTGGGTGCCTAGTGA+AGG | 0.596232 | 5.1:+29690177 | MS.gene074027:CDS |
| GCAGCATGCTGGAGATTCGT+CGG | 0.597982 | 5.1:+29689195 | MS.gene074027:CDS |
| TGCTGCTGATTATGCTGACA+TGG | 0.602364 | 5.1:+29689990 | MS.gene074027:CDS |
| GCGATGTCGATGAATGGTTG+GGG | 0.603196 | 5.1:+29690075 | MS.gene074027:CDS |
| AGCATTCATTCTCATTCCAG+AGG | 0.606098 | 5.1:-29689596 | None:intergenic |
| TGTTATTCAGCAATCTGATG+AGG | 0.609072 | 5.1:+29689171 | MS.gene074027:CDS |
| GTTTGTCAAGGAGTTCATGA+TGG | 0.609659 | 5.1:+29689121 | MS.gene074027:CDS |
| ACTCCTTGACAAACATTCAA+GGG | 0.609745 | 5.1:-29689112 | None:intergenic |
| TCAGCAGAATCAATATTCAT+CGG | 0.615157 | 5.1:-29688962 | None:intergenic |
| GAGCCCTTGAATGTTTGTCA+AGG | 0.615186 | 5.1:+29689109 | MS.gene074027:CDS |
| GGTCTTGCATTTAGGGCATG+CGG | 0.616506 | 5.1:+29690096 | MS.gene074027:CDS |
| ATTGGTGGACCTAGGACTCA+TGG | 0.617047 | 5.1:+29690135 | MS.gene074027:CDS |
| AACCCCTACTACTTCGTCCG+GGG | 0.618769 | 5.1:-29689906 | None:intergenic |
| ATAATTTACAAACCAGAACA+TGG | 0.619596 | 5.1:+29689863 | MS.gene074027:CDS |
| CTCAGAATTGTATAGTTCAG+CGG | 0.626222 | 5.1:-29689704 | None:intergenic |
| CTAGTGAAGGACCTCCGCAA+TGG | 0.629280 | 5.1:+29690190 | MS.gene074027:CDS |
| ACCCCTACTACTTCGTCCGG+GGG | 0.630121 | 5.1:-29689905 | None:intergenic |
| CGAGCTAGTAGACGCCATTG+CGG | 0.631973 | 5.1:-29690204 | None:intergenic |
| TTATCTACAACGGCAATGAG+AGG | 0.633005 | 5.1:-29689958 | None:intergenic |
| GCAGGTGGTTGTGATTCGGA+GGG | 0.633783 | 5.1:+29689667 | MS.gene074027:CDS |
| TCATTGTTATTGGTGGACCT+AGG | 0.638584 | 5.1:+29690127 | MS.gene074027:CDS |
| GGACCTAGGACTCATGGTGA+AGG | 0.644982 | 5.1:+29690141 | MS.gene074027:CDS |
| TTCCGTGAAATAATCCGAAG+CGG | 0.645763 | 5.1:+29689322 | MS.gene074027:CDS |
| GCGGCGAGGTTTATAGATGG+AGG | 0.647194 | 5.1:+29689341 | MS.gene074027:CDS |
| CGTACGACCCAATTCGCCAA+AGG | 0.656928 | 5.1:+29689425 | MS.gene074027:CDS |
| AAAGGTGGATGCATTTGCCG+AGG | 0.658304 | 5.1:+29689443 | MS.gene074027:CDS |
| GCTAGTAGACGCCATTGCGG+AGG | 0.660404 | 5.1:-29690201 | None:intergenic |
| ATACAATTCATTATCTACAA+CGG | 0.661324 | 5.1:-29689968 | None:intergenic |
| TGTCAAGGAGTTCATGATGG+TGG | 0.663431 | 5.1:+29689124 | MS.gene074027:CDS |
| TATGTTGTAGGTGGAATCGG+CGG | 0.671001 | 5.1:+29689805 | MS.gene074027:CDS |
| CGTACGCTTCCCATTCAAGG+AGG | 0.678294 | 5.1:-29689408 | None:intergenic |
| GTACGCTTCCCATTCAAGGA+GGG | 0.679998 | 5.1:-29689407 | None:intergenic |
| TCTACAACGGCAATGAGAGG+CGG | 0.681780 | 5.1:-29689955 | None:intergenic |
| GGAAGTTAAAAGGTATGACA+AGG | 0.683430 | 5.1:+29690011 | MS.gene074027:CDS |
| GATGAATATTGATTCTGCTG+AGG | 0.684071 | 5.1:+29688964 | MS.gene074027:CDS |
| CTAATATCGCAATCTCACCA+AGG | 0.689793 | 5.1:-29689645 | None:intergenic |
| ATGACAAGGAAAGAAAACTG+TGG | 0.706266 | 5.1:+29690025 | MS.gene074027:CDS |
| TCCCGAGCATGAATAAACCG+AGG | 0.713762 | 5.1:+29689746 | MS.gene074027:CDS |
| GATTCAAAGCTTCTTACATG+CGG | 0.719497 | 5.1:+29689832 | MS.gene074027:CDS |
| GAGATTGCGATATTAGCAGG+TGG | 0.734472 | 5.1:+29689652 | MS.gene074027:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACAATTCATTATCTACAA+CGG | - | chr5.1:29689971-29689990 | None:intergenic | 20.0% |
| ! | ACAATTATAAACGAAGTTAC+CGG | - | chr5.1:29690238-29690257 | None:intergenic | 25.0% |
| ! | ATAATTTACAAACCAGAACA+TGG | + | chr5.1:29689863-29689882 | MS.gene074027:CDS | 25.0% |
| ! | TTTCATTGAACTCAATTCTT+GGG | + | chr5.1:29690164-29690183 | MS.gene074027:CDS | 25.0% |
| !! | GATGGAAAATTTTATGTTGT+AGG | + | chr5.1:29689793-29689812 | MS.gene074027:CDS | 25.0% |
| ATCTTGTCACATAGTTGAAA+CGG | + | chr5.1:29689024-29689043 | MS.gene074027:CDS | 30.0% | |
| GATAAAGAATCTTTAGCTGT+AGG | + | chr5.1:29689496-29689515 | MS.gene074027:CDS | 30.0% | |
| GGAAATATGCTCATTGTTAT+TGG | + | chr5.1:29690117-29690136 | MS.gene074027:CDS | 30.0% | |
| GTTTATAATTGTGCTGTGAT+GGG | + | chr5.1:29690246-29690265 | MS.gene074027:CDS | 30.0% | |
| GTTTCATTGAACTCAATTCT+TGG | + | chr5.1:29690163-29690182 | MS.gene074027:CDS | 30.0% | |
| TCAGCAGAATCAATATTCAT+CGG | - | chr5.1:29688965-29688984 | None:intergenic | 30.0% | |
| ! | AATATGCTCATTGTTATTGG+TGG | + | chr5.1:29690120-29690139 | MS.gene074027:CDS | 30.0% |
| ! | ACAGTCTTTTGACAAATTCA+TGG | + | chr5.1:29689572-29689591 | MS.gene074027:CDS | 30.0% |
| ! | GGAAAATTTTATGTTGTAGG+TGG | + | chr5.1:29689796-29689815 | MS.gene074027:CDS | 30.0% |
| ! | TTTTATGTTGTAGGTGGAAT+CGG | + | chr5.1:29689802-29689821 | MS.gene074027:CDS | 30.0% |
| AAACGGTATCATGGAACATT+GGG | + | chr5.1:29689369-29689388 | MS.gene074027:CDS | 35.0% | |
| AACATCTTAGTACACTTCCT+AGG | - | chr5.1:29689001-29689020 | None:intergenic | 35.0% | |
| AACTCCTTGACAAACATTCA+AGG | - | chr5.1:29689116-29689135 | None:intergenic | 35.0% | |
| AATGCTCCAAGATGTTTGTT+CGG | + | chr5.1:29689613-29689632 | MS.gene074027:CDS | 35.0% | |
| ACTCCTTGACAAACATTCAA+GGG | - | chr5.1:29689115-29689134 | None:intergenic | 35.0% | |
| ATGACAAGGAAAGAAAACTG+TGG | + | chr5.1:29690025-29690044 | MS.gene074027:CDS | 35.0% | |
| ATGCTGACATGGAAGTTAAA+AGG | + | chr5.1:29690001-29690020 | MS.gene074027:CDS | 35.0% | |
| CGTTTATAATTGTGCTGTGA+TGG | + | chr5.1:29690245-29690264 | MS.gene074027:CDS | 35.0% | |
| CTCAGAATTGTATAGTTCAG+CGG | - | chr5.1:29689707-29689726 | None:intergenic | 35.0% | |
| CTGGATTTGGACATCAAAAA+TGG | + | chr5.1:29688932-29688951 | MS.gene074027:CDS | 35.0% | |
| GAACTGAACTTCTTGTGTTT+GGG | + | chr5.1:29689518-29689537 | MS.gene074027:CDS | 35.0% | |
| GATTCAAAGCTTCTTACATG+CGG | + | chr5.1:29689832-29689851 | MS.gene074027:CDS | 35.0% | |
| GGATTCTGTTCTTTCTCAAA+CGG | + | chr5.1:29689072-29689091 | MS.gene074027:CDS | 35.0% | |
| TAAACGGTATCATGGAACAT+TGG | + | chr5.1:29689368-29689387 | MS.gene074027:CDS | 35.0% | |
| TATAGATGGAGGAGACTAAA+CGG | + | chr5.1:29689352-29689371 | MS.gene074027:CDS | 35.0% | |
| TATTTCGTTGCAACATCCTA+AGG | + | chr5.1:29689051-29689070 | MS.gene074027:CDS | 35.0% | |
| TGAGAAAGAACAGAATCCTT+AGG | - | chr5.1:29689070-29689089 | None:intergenic | 35.0% | |
| TGTTATTCAGCAATCTGATG+AGG | + | chr5.1:29689171-29689190 | MS.gene074027:CDS | 35.0% | |
| ! | AGAAAACTGTGGATTACTGT+TGG | + | chr5.1:29690036-29690055 | MS.gene074027:CDS | 35.0% |
| ! | GGAAGTTAAAAGGTATGACA+AGG | + | chr5.1:29690011-29690030 | MS.gene074027:CDS | 35.0% |
| ! | TAGGAAGTGTACTAAGATGT+TGG | + | chr5.1:29689000-29689019 | MS.gene074027:CDS | 35.0% |
| !! | GATGAATATTGATTCTGCTG+AGG | + | chr5.1:29688964-29688983 | MS.gene074027:CDS | 35.0% |
| ACAAACATTCAAGGGCTCAT+TGG | - | chr5.1:29689107-29689126 | None:intergenic | 40.0% | |
| AGCATTCATTCTCATTCCAG+AGG | - | chr5.1:29689599-29689618 | None:intergenic | 40.0% | |
| AGGAATTTCTGTCCATGTTC+TGG | - | chr5.1:29689878-29689897 | None:intergenic | 40.0% | |
| ATTAGCAGGTGGTTGTGATT+CGG | + | chr5.1:29689663-29689682 | MS.gene074027:CDS | 40.0% | |
| CATGAAGTACATGCTGGATT+TGG | + | chr5.1:29688919-29688938 | MS.gene074027:CDS | 40.0% | |
| CTAATATCGCAATCTCACCA+AGG | - | chr5.1:29689648-29689667 | None:intergenic | 40.0% | |
| CTTGTGAAACAGAGAACGAT+TGG | + | chr5.1:29688891-29688910 | MS.gene074027:CDS | 40.0% | |
| GATGTGTTCTGGTGTATTCA+TGG | + | chr5.1:29689771-29689790 | MS.gene074027:CDS | 40.0% | |
| GGAACTGAACTTCTTGTGTT+TGG | + | chr5.1:29689517-29689536 | MS.gene074027:CDS | 40.0% | |
| GTCTTACATGAAGTACATGC+TGG | + | chr5.1:29688913-29688932 | MS.gene074027:CDS | 40.0% | |
| GTTTGTCAAGGAGTTCATGA+TGG | + | chr5.1:29689121-29689140 | MS.gene074027:CDS | 40.0% | |
| TGAACTTCTTGTGTTTGGGA+GGG | + | chr5.1:29689522-29689541 | MS.gene074027:CDS | 40.0% | |
| TGTTCTGGTGTATTCATGGA+TGG | + | chr5.1:29689775-29689794 | MS.gene074027:CDS | 40.0% | |
| TTATCTACAACGGCAATGAG+AGG | - | chr5.1:29689961-29689980 | None:intergenic | 40.0% | |
| TTCCGTGAAATAATCCGAAG+CGG | + | chr5.1:29689322-29689341 | MS.gene074027:CDS | 40.0% | |
| TTCCTCGGTTTATTCATGCT+CGG | - | chr5.1:29689751-29689770 | None:intergenic | 40.0% | |
| TTGACAAATTCATGGTCCTC+TGG | + | chr5.1:29689580-29689599 | MS.gene074027:CDS | 40.0% | |
| ! | TCATTGTTATTGGTGGACCT+AGG | + | chr5.1:29690127-29690146 | MS.gene074027:CDS | 40.0% |
| !!! | ATTCTGGTTCTCTTTTACCG+AGG | + | chr5.1:29689218-29689237 | MS.gene074027:CDS | 40.0% |
| AAACCGAGGAAGATGTGTTC+TGG | + | chr5.1:29689760-29689779 | MS.gene074027:CDS | 45.0% | |
| AAACCTTCACCATGAGTCCT+AGG | - | chr5.1:29690147-29690166 | None:intergenic | 45.0% | |
| AATTCTTGGGTGCCTAGTGA+AGG | + | chr5.1:29690177-29690196 | MS.gene074027:CDS | 45.0% | |
| ACACCAGAACACATCTTCCT+CGG | - | chr5.1:29689766-29689785 | None:intergenic | 45.0% | |
| AGCGATGTCGATGAATGGTT+GGG | + | chr5.1:29690074-29690093 | MS.gene074027:CDS | 45.0% | |
| ATCTATAAACCTCGCCGCTT+CGG | - | chr5.1:29689339-29689358 | None:intergenic | 45.0% | |
| ATGGTTGGGGTCTTGCATTT+AGG | + | chr5.1:29690088-29690107 | MS.gene074027:CDS | 45.0% | |
| CGCTGTTCAAGGTCTGATTA+TGG | + | chr5.1:29689274-29689293 | MS.gene074027:CDS | 45.0% | |
| CTACTAGCTCGAAAACGATC+CGG | + | chr5.1:29690216-29690235 | MS.gene074027:CDS | 45.0% | |
| CTGAACTTCTTGTGTTTGGG+AGG | + | chr5.1:29689521-29689540 | MS.gene074027:CDS | 45.0% | |
| CTGAGGATGAACATCATCCT+AGG | + | chr5.1:29688981-29689000 | MS.gene074027:CDS | 45.0% | |
| GAGATTGCGATATTAGCAGG+TGG | + | chr5.1:29689652-29689671 | MS.gene074027:CDS | 45.0% | |
| GAGCCCTTGAATGTTTGTCA+AGG | + | chr5.1:29689109-29689128 | MS.gene074027:CDS | 45.0% | |
| GAGGAGACTAAACGGTATCA+TGG | + | chr5.1:29689360-29689379 | MS.gene074027:CDS | 45.0% | |
| GGTGAGATTGCGATATTAGC+AGG | + | chr5.1:29689649-29689668 | MS.gene074027:CDS | 45.0% | |
| TATGTTGTAGGTGGAATCGG+CGG | + | chr5.1:29689805-29689824 | MS.gene074027:CDS | 45.0% | |
| TCCTCGGTTTATTCATGCTC+GGG | - | chr5.1:29689750-29689769 | None:intergenic | 45.0% | |
| TGGTTGGGGTCTTGCATTTA+GGG | + | chr5.1:29690089-29690108 | MS.gene074027:CDS | 45.0% | |
| TGTCAAGGAGTTCATGATGG+TGG | + | chr5.1:29689124-29689143 | MS.gene074027:CDS | 45.0% | |
| ! | CAATCGTTTGAAGCCATCCT+CGG | - | chr5.1:29689463-29689482 | None:intergenic | 45.0% |
| ! | TGCTGCTGATTATGCTGACA+TGG | + | chr5.1:29689990-29690009 | MS.gene074027:CDS | 45.0% |
| AAAGGTGGATGCATTTGCCG+AGG | + | chr5.1:29689443-29689462 | MS.gene074027:CDS | 50.0% | |
| AGAACCCCTACTACTTCGTC+CGG | - | chr5.1:29689911-29689930 | None:intergenic | 50.0% | |
| CGCCGCTTCGGATTATTTCA+CGG | - | chr5.1:29689327-29689346 | None:intergenic | 50.0% | |
| GAAATTCCTGACATGTCCCC+CGG | + | chr5.1:29689889-29689908 | MS.gene074027:CDS | 50.0% | |
| GAACGAGCGATGTCGATGAA+TGG | + | chr5.1:29690069-29690088 | MS.gene074027:CDS | 50.0% | |
| GAAGCGGCGAGGTTTATAGA+TGG | + | chr5.1:29689338-29689357 | MS.gene074027:CDS | 50.0% | |
| GACACACGATTGAGCTGTCT+CGG | - | chr5.1:29689249-29689268 | None:intergenic | 50.0% | |
| GAGCGATGTCGATGAATGGT+TGG | + | chr5.1:29690073-29690092 | MS.gene074027:CDS | 50.0% | |
| GCAGAGCCGAACAAACATCT+TGG | - | chr5.1:29689622-29689641 | None:intergenic | 50.0% | |
| GCATCCACCTTTGGCGAATT+GGG | - | chr5.1:29689435-29689454 | None:intergenic | 50.0% | |
| GCGATGTCGATGAATGGTTG+GGG | + | chr5.1:29690075-29690094 | MS.gene074027:CDS | 50.0% | |
| GTACGCTTCCCATTCAAGGA+GGG | - | chr5.1:29689410-29689429 | None:intergenic | 50.0% | |
| TCCCGAGCATGAATAAACCG+AGG | + | chr5.1:29689746-29689765 | MS.gene074027:CDS | 50.0% | |
| TCGGCAAATGCATCCACCTT+TGG | - | chr5.1:29689444-29689463 | None:intergenic | 50.0% | |
| TCTACAACGGCAATGAGAGG+CGG | - | chr5.1:29689958-29689977 | None:intergenic | 50.0% | |
| TGAAATAATCCGAAGCGGCG+AGG | + | chr5.1:29689327-29689346 | MS.gene074027:CDS | 50.0% | |
| TGCATCCACCTTTGGCGAAT+TGG | - | chr5.1:29689436-29689455 | None:intergenic | 50.0% | |
| TGTGTCTCAGTCGCTGTTCA+AGG | + | chr5.1:29689263-29689282 | MS.gene074027:CDS | 50.0% | |
| TTCATCGACATCGCTCGTTC+TGG | - | chr5.1:29690069-29690088 | None:intergenic | 50.0% | |
| ! | ATTGGTGGACCTAGGACTCA+TGG | + | chr5.1:29690135-29690154 | MS.gene074027:CDS | 50.0% |
| ! | GGTCTTGCATTTAGGGCATG+CGG | + | chr5.1:29690096-29690115 | MS.gene074027:CDS | 50.0% |
| !! | GTCGTTGCTGTGTTGTTTCG+AGG | + | chr5.1:29688855-29688874 | MS.gene074027:CDS | 50.0% |
| AACCCCTACTACTTCGTCCG+GGG | - | chr5.1:29689909-29689928 | None:intergenic | 55.0% | |
| ACGACCCAATTCGCCAAAGG+TGG | + | chr5.1:29689428-29689447 | MS.gene074027:CDS | 55.0% | |
| CATTGCGGAGGTCCTTCACT+AGG | - | chr5.1:29690192-29690211 | None:intergenic | 55.0% | |
| CGAGCTAGTAGACGCCATTG+CGG | - | chr5.1:29690207-29690226 | None:intergenic | 55.0% | |
| CGTACGACCCAATTCGCCAA+AGG | + | chr5.1:29689425-29689444 | MS.gene074027:CDS | 55.0% | |
| CGTACGCTTCCCATTCAAGG+AGG | - | chr5.1:29689411-29689430 | None:intergenic | 55.0% | |
| CTAGTGAAGGACCTCCGCAA+TGG | + | chr5.1:29690190-29690209 | MS.gene074027:CDS | 55.0% | |
| CTCGTGTGCCCTCCTTGAAT+GGG | + | chr5.1:29689399-29689418 | MS.gene074027:CDS | 55.0% | |
| GAACCCCTACTACTTCGTCC+GGG | - | chr5.1:29689910-29689929 | None:intergenic | 55.0% | |
| GAGCTGTCTCGGTTCATCCT+CGG | - | chr5.1:29689238-29689257 | None:intergenic | 55.0% | |
| GCAGCATGCTGGAGATTCGT+CGG | + | chr5.1:29689195-29689214 | MS.gene074027:CDS | 55.0% | |
| GCGGCGAGGTTTATAGATGG+AGG | + | chr5.1:29689341-29689360 | MS.gene074027:CDS | 55.0% | |
| GCTGGAGATTCGTCGGATTC+TGG | + | chr5.1:29689202-29689221 | MS.gene074027:CDS | 55.0% | |
| GGACCTAGGACTCATGGTGA+AGG | + | chr5.1:29690141-29690160 | MS.gene074027:CDS | 55.0% | |
| GGTCGTACGCTTCCCATTCA+AGG | - | chr5.1:29689414-29689433 | None:intergenic | 55.0% | |
| GTGGATGCATTTGCCGAGGA+TGG | + | chr5.1:29689447-29689466 | MS.gene074027:CDS | 55.0% | |
| TCTCGTGTGCCCTCCTTGAA+TGG | + | chr5.1:29689398-29689417 | MS.gene074027:CDS | 55.0% | |
| TCTGATGAGGAGCAGCATGC+TGG | + | chr5.1:29689184-29689203 | MS.gene074027:CDS | 55.0% | |
| TTGTTCGGCTCTGCAAGCCT+TGG | + | chr5.1:29689628-29689647 | MS.gene074027:CDS | 55.0% | |
| ! | AGCAGGTGGTTGTGATTCGG+AGG | + | chr5.1:29689666-29689685 | MS.gene074027:CDS | 55.0% |
| ! | GCAGGTGGTTGTGATTCGGA+GGG | + | chr5.1:29689667-29689686 | MS.gene074027:CDS | 55.0% |
| ACCCCTACTACTTCGTCCGG+GGG | - | chr5.1:29689908-29689927 | None:intergenic | 60.0% | |
| GCTAGTAGACGCCATTGCGG+AGG | - | chr5.1:29690204-29690223 | None:intergenic | 60.0% | |
| GTCCCCCGGACGAAGTAGTA+GGG | + | chr5.1:29689903-29689922 | MS.gene074027:CDS | 60.0% | |
| TCCCCCGGACGAAGTAGTAG+GGG | + | chr5.1:29689904-29689923 | MS.gene074027:CDS | 60.0% | |
| TGAGATGCCTGCTGCAACCG+AGG | + | chr5.1:29689930-29689949 | MS.gene074027:CDS | 60.0% | |
| TGTCCCCCGGACGAAGTAGT+AGG | + | chr5.1:29689902-29689921 | MS.gene074027:CDS | 60.0% | |
| CTTCGTCCGGGGGACATGTC+AGG | - | chr5.1:29689898-29689917 | None:intergenic | 65.0% | |
| GGCAATGAGAGGCGGTGCCT+CGG | - | chr5.1:29689950-29689969 | None:intergenic | 65.0% | |
| GGCGGTGCCTCGGTTGCAGC+AGG | - | chr5.1:29689940-29689959 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 29688845 | 29690275 | 29688845 | ID=MS.gene074027 |
| chr5.1 | mRNA | 29688845 | 29690275 | 29688845 | ID=MS.gene074027.t1;Parent=MS.gene074027 |
| chr5.1 | exon | 29688845 | 29690275 | 29688845 | ID=MS.gene074027.t1.exon1;Parent=MS.gene074027.t1 |
| chr5.1 | CDS | 29688845 | 29690275 | 29688845 | ID=cds.MS.gene074027.t1;Parent=MS.gene074027.t1 |
| Gene Sequence |
| Protein sequence |
