Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91909.t1 | XP_003613674.2 | 98.1 | 476 | 9 | 0 | 1 | 476 | 1 | 476 | 8.00E-262 | 912.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91909.t1 | Q8L736 | 59.9 | 486 | 166 | 10 | 1 | 476 | 1 | 467 | 4.3e-139 | 496.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91909.t1 | G7KCH5 | 98.1 | 476 | 9 | 0 | 1 | 476 | 1 | 476 | 5.8e-262 | 912.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene91909 | MS.gene63251 | PPI |
| MS.gene91909 | MS.gene06516 | PPI |
| MS.gene91909 | MS.gene055886 | PPI |
| MS.gene91909 | MS.gene013419 | PPI |
| MS.gene074027 | MS.gene91909 | PPI |
| MS.gene91909 | MS.gene34711 | PPI |
| MS.gene91909 | MS.gene83931 | PPI |
| MS.gene91909 | MS.gene25443 | PPI |
| MS.gene91909 | MS.gene032830 | PPI |
| MS.gene059347 | MS.gene91909 | PPI |
| MS.gene91909 | MS.gene049391 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91909.t1 | MTR_5g039390 | 98.319 | 476 | 8 | 0 | 1 | 476 | 1 | 476 | 0.0 | 972 |
| MS.gene91909.t1 | MTR_8g468030 | 73.870 | 509 | 88 | 7 | 1 | 476 | 1 | 497 | 0.0 | 734 |
| MS.gene91909.t1 | MTR_4g108280 | 63.760 | 367 | 128 | 2 | 112 | 476 | 17 | 380 | 5.42e-173 | 491 |
| MS.gene91909.t1 | MTR_4g022260 | 60.656 | 366 | 143 | 1 | 111 | 476 | 53 | 417 | 6.10e-161 | 462 |
| MS.gene91909.t1 | MTR_0345s0010 | 61.190 | 353 | 136 | 1 | 124 | 476 | 84 | 435 | 7.17e-161 | 462 |
| MS.gene91909.t1 | MTR_0105s0130 | 61.190 | 353 | 136 | 1 | 124 | 476 | 84 | 435 | 2.02e-160 | 461 |
| MS.gene91909.t1 | MTR_0105s0170 | 60.907 | 353 | 137 | 1 | 124 | 476 | 97 | 448 | 9.58e-160 | 460 |
| MS.gene91909.t1 | MTR_0105s0020 | 60.340 | 353 | 139 | 1 | 124 | 476 | 84 | 435 | 3.15e-159 | 458 |
| MS.gene91909.t1 | MTR_0105s0040 | 60.907 | 353 | 137 | 1 | 124 | 476 | 182 | 533 | 4.02e-159 | 461 |
| MS.gene91909.t1 | MTR_0496s0010 | 59.563 | 366 | 141 | 3 | 111 | 476 | 53 | 411 | 6.89e-158 | 454 |
| MS.gene91909.t1 | MTR_1330s0010 | 60.807 | 347 | 135 | 1 | 124 | 470 | 97 | 442 | 6.18e-156 | 450 |
| MS.gene91909.t1 | MTR_4g022400 | 59.322 | 354 | 143 | 1 | 123 | 476 | 62 | 414 | 1.04e-151 | 438 |
| MS.gene91909.t1 | MTR_2g094093 | 50.282 | 354 | 163 | 4 | 128 | 476 | 19 | 364 | 2.87e-115 | 343 |
| MS.gene91909.t1 | MTR_7g029290 | 38.596 | 342 | 193 | 9 | 142 | 474 | 68 | 401 | 2.06e-66 | 219 |
| MS.gene91909.t1 | MTR_6g023805 | 37.534 | 365 | 190 | 9 | 130 | 474 | 57 | 403 | 2.40e-63 | 211 |
| MS.gene91909.t1 | MTR_0105s0060 | 56.044 | 91 | 40 | 0 | 386 | 476 | 15 | 105 | 6.31e-35 | 126 |
| MS.gene91909.t1 | MTR_3g085570 | 28.980 | 245 | 149 | 7 | 125 | 352 | 31 | 267 | 9.75e-22 | 97.1 |
| MS.gene91909.t1 | MTR_8g080370 | 26.446 | 242 | 159 | 5 | 125 | 352 | 47 | 283 | 2.16e-16 | 81.3 |
| MS.gene91909.t1 | MTR_3g073640 | 23.851 | 348 | 191 | 11 | 129 | 408 | 43 | 384 | 1.34e-15 | 79.3 |
| MS.gene91909.t1 | MTR_8g085600 | 26.408 | 284 | 181 | 9 | 129 | 401 | 77 | 343 | 2.76e-15 | 78.2 |
| MS.gene91909.t1 | MTR_4g114110 | 25.243 | 309 | 201 | 11 | 105 | 401 | 57 | 347 | 5.28e-15 | 77.4 |
| MS.gene91909.t1 | MTR_2g008440 | 23.810 | 357 | 191 | 12 | 125 | 408 | 39 | 387 | 1.47e-13 | 73.2 |
| MS.gene91909.t1 | MTR_4g011900 | 24.800 | 250 | 162 | 5 | 129 | 352 | 5 | 254 | 2.00e-13 | 72.0 |
| MS.gene91909.t1 | MTR_7g095220 | 22.511 | 231 | 161 | 4 | 138 | 351 | 13 | 242 | 5.72e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91909.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene91909.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene91909.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene91909.t1 | AT1G14330 | 59.290 | 479 | 154 | 8 | 1 | 476 | 1 | 441 | 0.0 | 550 |
| MS.gene91909.t1 | AT1G26930 | 68.378 | 370 | 114 | 1 | 110 | 476 | 52 | 421 | 0.0 | 531 |
| MS.gene91909.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene91909.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene91909.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene91909.t1 | AT1G26930 | 57.838 | 370 | 101 | 2 | 110 | 476 | 52 | 369 | 4.88e-149 | 430 |
| MS.gene91909.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene91909.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene91909.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene91909.t1 | AT3G27150 | 37.673 | 361 | 198 | 10 | 130 | 474 | 71 | 420 | 9.55e-67 | 220 |
| MS.gene91909.t1 | AT3G27150 | 37.673 | 361 | 198 | 10 | 130 | 474 | 71 | 420 | 9.55e-67 | 220 |
| MS.gene91909.t1 | AT5G40680 | 35.119 | 336 | 206 | 4 | 107 | 437 | 43 | 371 | 4.92e-61 | 205 |
| MS.gene91909.t1 | AT1G16250 | 26.061 | 330 | 208 | 10 | 128 | 433 | 7 | 324 | 2.97e-24 | 104 |
| MS.gene91909.t1 | AT1G16250 | 26.061 | 330 | 208 | 10 | 128 | 433 | 7 | 324 | 2.97e-24 | 104 |
| MS.gene91909.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene91909.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene91909.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene91909.t1 | AT1G15670 | 25.648 | 347 | 209 | 14 | 129 | 445 | 3 | 330 | 7.49e-17 | 82.4 |
| MS.gene91909.t1 | AT1G55270 | 26.496 | 234 | 157 | 5 | 129 | 352 | 78 | 306 | 1.71e-16 | 82.0 |
| MS.gene91909.t1 | AT3G61350 | 23.054 | 334 | 215 | 12 | 115 | 432 | 6 | 313 | 2.20e-14 | 74.7 |
| MS.gene91909.t1 | AT3G61350 | 23.054 | 334 | 215 | 12 | 115 | 432 | 6 | 313 | 2.20e-14 | 74.7 |
| MS.gene91909.t1 | AT1G80440 | 24.540 | 326 | 208 | 11 | 129 | 432 | 3 | 312 | 7.52e-14 | 73.2 |
| MS.gene91909.t1 | AT1G22040 | 25.862 | 348 | 183 | 11 | 129 | 408 | 43 | 383 | 7.56e-14 | 73.9 |
| MS.gene91909.t1 | AT1G27420 | 24.206 | 252 | 150 | 7 | 124 | 350 | 5 | 240 | 4.60e-12 | 67.8 |
| MS.gene91909.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 4 | 214 | 2.97e-11 | 65.1 |
| MS.gene91909.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 11 | 221 | 3.01e-11 | 65.1 |
| MS.gene91909.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 11 | 221 | 3.01e-11 | 65.1 |
Find 119 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGGAGATTCGTCGGATTC+TGG | 0.248232 | 5.3:+27623253 | MS.gene91909:CDS |
| TGCATCCACCTTTGGCGAAT+TGG | 0.251940 | 5.3:-27623484 | None:intergenic |
| ATGGTTGGGGTCTTGCATTT+AGG | 0.276947 | 5.3:+27624139 | MS.gene91909:CDS |
| TGGTTGGGGTCTTGCATTTA+GGG | 0.285142 | 5.3:+27624140 | MS.gene91909:CDS |
| GGAAATATGCTCATTGTTAT+TGG | 0.306561 | 5.3:+27624168 | MS.gene91909:CDS |
| CATGAAGTACATGCTGGATT+TGG | 0.313702 | 5.3:+27622970 | MS.gene91909:CDS |
| TTCATCGACATCGCTCGTTC+TGG | 0.316008 | 5.3:-27624117 | None:intergenic |
| GGAACCGAACTTCTTGTGTT+TGG | 0.324378 | 5.3:+27623568 | MS.gene91909:CDS |
| GTTTCATTGAACTCAATTCT+TGG | 0.330175 | 5.3:+27624214 | MS.gene91909:CDS |
| AGGAATTTCTGTCCATGTTC+TGG | 0.338733 | 5.3:-27623926 | None:intergenic |
| ATCTATAAACCTCGCCGCTT+CGG | 0.350189 | 5.3:-27623387 | None:intergenic |
| ATGCTGACATGGAAGTTAAA+AGG | 0.357590 | 5.3:+27624052 | MS.gene91909:CDS |
| GAACCGAACTTCTTGTGTTT+GGG | 0.358419 | 5.3:+27623569 | MS.gene91909:CDS |
| GCATCCACCTTTGGCGAATT+GGG | 0.360483 | 5.3:-27623483 | None:intergenic |
| CGCTGTTCAAGGTCTGATTA+TGG | 0.366547 | 5.3:+27623325 | MS.gene91909:CDS |
| TGTGTCTCAGTCGCTGTTCA+AGG | 0.376165 | 5.3:+27623314 | MS.gene91909:CDS |
| TCTCGTGTGCCCTCCTTGAA+TGG | 0.387707 | 5.3:+27623449 | MS.gene91909:CDS |
| TTTCATTGAACTCAATTCTT+GGG | 0.403492 | 5.3:+27624215 | MS.gene91909:CDS |
| TTGACAAATTCATGGTCCTC+TGG | 0.419803 | 5.3:+27623631 | MS.gene91909:CDS |
| TCGGCAAATGCATCCACCTT+TGG | 0.425014 | 5.3:-27623492 | None:intergenic |
| CGTTTATAATTGTGCTGTGA+TGG | 0.426012 | 5.3:+27624296 | MS.gene91909:CDS |
| ACAAACATTCAAGGGCTCAT+TGG | 0.427663 | 5.3:-27623155 | None:intergenic |
| TCAGCAGAATCAATATTCAT+TGG | 0.430740 | 5.3:-27623013 | None:intergenic |
| CTCGTGTGCCCTCCTTGAAT+GGG | 0.432187 | 5.3:+27623450 | MS.gene91909:CDS |
| TTCCTCGGTTTATTCATGCT+CGG | 0.436739 | 5.3:-27623799 | None:intergenic |
| GGCGGTGCCTCGGTTGCAGC+AGG | 0.445232 | 5.3:-27623988 | None:intergenic |
| AAACCGAGGAAGATGTGTTC+TGG | 0.445291 | 5.3:+27623811 | MS.gene91909:CDS |
| ATTAGCAGGTGGTTGTGATT+CGG | 0.446104 | 5.3:+27623714 | MS.gene91909:CDS |
| TCCTCGGTTTATTCATGCTC+GGG | 0.446460 | 5.3:-27623798 | None:intergenic |
| GGATTCTGTTCTTTCTCAAA+CGG | 0.450478 | 5.3:+27623123 | MS.gene91909:CDS |
| AGCGATGTCGATGAATGGTT+GGG | 0.451823 | 5.3:+27624125 | MS.gene91909:CDS |
| AAACGGTATCATGGAACATT+GGG | 0.454394 | 5.3:+27623420 | MS.gene91909:CDS |
| ACAATTATAAACGAAGTTAC+CGG | 0.457419 | 5.3:-27624286 | None:intergenic |
| GGTCGTACGCTTCCCATTCA+AGG | 0.463650 | 5.3:-27623462 | None:intergenic |
| GATGTGTTCTGGTGTATTCA+TGG | 0.466838 | 5.3:+27623822 | MS.gene91909:CDS |
| AGAACCCCTACTACTTCGTC+CGG | 0.468311 | 5.3:-27623959 | None:intergenic |
| TGAGAAAGAACAGAATCCTT+AGG | 0.471913 | 5.3:-27623118 | None:intergenic |
| AACTCCTTGACAAACATTCA+AGG | 0.473412 | 5.3:-27623164 | None:intergenic |
| AATGCTCCAAGATGTTTGTT+CGG | 0.474359 | 5.3:+27623664 | MS.gene91909:CDS |
| CGCCGCTTCGGATTATTTCA+CGG | 0.477509 | 5.3:-27623375 | None:intergenic |
| GAGCGATGTCGATGAATGGT+TGG | 0.478296 | 5.3:+27624124 | MS.gene91909:CDS |
| ATCTTGTCACATAGTTGAAA+CGG | 0.478670 | 5.3:+27623075 | MS.gene91909:CDS |
| TATTTCGTTGCAACATCCTA+AGG | 0.480105 | 5.3:+27623102 | MS.gene91909:CDS |
| GTCGTTGCTGTGTTGTTTCG+AGG | 0.487767 | 5.3:+27622906 | MS.gene91909:CDS |
| TGAGATGCCTGCTGCAACCG+AGG | 0.492241 | 5.3:+27623981 | MS.gene91909:CDS |
| GAAGCGGCGAGGTTTATAGA+TGG | 0.493954 | 5.3:+27623389 | MS.gene91909:CDS |
| GGTGAGATTGCGATATTAGC+AGG | 0.494746 | 5.3:+27623700 | MS.gene91909:CDS |
| AAACCTTCACCATGAGTCCT+AGG | 0.500082 | 5.3:-27624195 | None:intergenic |
| GATAAAGAATCTTTAGCTGT+AGG | 0.501866 | 5.3:+27623547 | MS.gene91909:CDS |
| CTGAAGATGAACATCATCCT+AGG | 0.506033 | 5.3:+27623032 | MS.gene91909:CDS |
| GTCTTACATGAAGTACATGC+TGG | 0.509303 | 5.3:+27622964 | MS.gene91909:CDS |
| GGCAATGAGAGGCGGTGCCT+CGG | 0.509621 | 5.3:-27623998 | None:intergenic |
| AACATCTTAGTACACTTCCT+AGG | 0.510935 | 5.3:-27623049 | None:intergenic |
| AATATGCTCATTGTTATTGG+TGG | 0.514731 | 5.3:+27624171 | MS.gene91909:CDS |
| CCGAACTTCTTGTGTTTGGG+AGG | 0.516315 | 5.3:+27623572 | MS.gene91909:CDS |
| TCTGATGAGGAGCAGCATGC+TGG | 0.518838 | 5.3:+27623235 | MS.gene91909:CDS |
| CCTCCCAAACACAAGAAGTT+CGG | 0.521950 | 5.3:-27623572 | None:intergenic |
| GTGGATGCATTTGCCGAGGA+TGG | 0.521975 | 5.3:+27623498 | MS.gene91909:CDS |
| GCAGAGCCGAACAAACATCT+TGG | 0.522841 | 5.3:-27623670 | None:intergenic |
| TATAGATGGAGGAGACTAAA+CGG | 0.529185 | 5.3:+27623403 | MS.gene91909:CDS |
| GTTTATAATTGTGCTGTGAT+GGG | 0.533513 | 5.3:+27624297 | MS.gene91909:CDS |
| TAAACGGTATCATGGAACAT+TGG | 0.533894 | 5.3:+27623419 | MS.gene91909:CDS |
| GAGGAGACTAAACGGTATCA+TGG | 0.537620 | 5.3:+27623411 | MS.gene91909:CDS |
| GAACGAGCGATGTCGATGAA+TGG | 0.544102 | 5.3:+27624120 | MS.gene91909:CDS |
| CGAACTTCTTGTGTTTGGGA+GGG | 0.544855 | 5.3:+27623573 | MS.gene91909:CDS |
| TAGGAAGTGTACTAAGATGT+TGG | 0.544939 | 5.3:+27623051 | MS.gene91909:CDS |
| GAACCCCTACTACTTCGTCC+GGG | 0.545858 | 5.3:-27623958 | None:intergenic |
| ACACCAGAACACATCTTCCT+CGG | 0.549483 | 5.3:-27623814 | None:intergenic |
| GACACACGATTGAGCTGTCT+CGG | 0.549997 | 5.3:-27623297 | None:intergenic |
| TTGTTCGGCTCTGCAAGCCT+TGG | 0.557116 | 5.3:+27623679 | MS.gene91909:CDS |
| CTACTAGCTCGAAAACGATC+CGG | 0.564520 | 5.3:+27624267 | MS.gene91909:CDS |
| GAAATTCCTGACATGTCCCC+CGG | 0.564869 | 5.3:+27623940 | MS.gene91909:CDS |
| CTTGTGAAACAGAGAACGAT+TGG | 0.567058 | 5.3:+27622942 | MS.gene91909:CDS |
| GAGCTGTCTCGGTTCATCCT+CGG | 0.567515 | 5.3:-27623286 | None:intergenic |
| AGCAGGTGGTTGTGATTCGG+AGG | 0.569771 | 5.3:+27623717 | MS.gene91909:CDS |
| CATTGTGGAGGTCCTTCACT+AGG | 0.572615 | 5.3:-27624240 | None:intergenic |
| CAATCGTTTGAAGCCATCCT+CGG | 0.574471 | 5.3:-27623511 | None:intergenic |
| TGTTCTGGTGTATTCATGGA+TGG | 0.587848 | 5.3:+27623826 | MS.gene91909:CDS |
| AGAAAACTGTGGATTACTGT+TGG | 0.588100 | 5.3:+27624087 | MS.gene91909:CDS |
| TGAAATAATCCGAAGCGGCG+AGG | 0.588835 | 5.3:+27623378 | MS.gene91909:CDS |
| ACGACCCAATTCGCCAAAGG+TGG | 0.595170 | 5.3:+27623479 | MS.gene91909:CDS |
| AATTCTTGGGTGCCTAGTGA+AGG | 0.596232 | 5.3:+27624228 | MS.gene91909:CDS |
| GCAGCATGCTGGAGATTCGT+CGG | 0.597982 | 5.3:+27623246 | MS.gene91909:CDS |
| TGCTGCTGATTATGCTGACA+TGG | 0.602364 | 5.3:+27624041 | MS.gene91909:CDS |
| GCGATGTCGATGAATGGTTG+GGG | 0.603196 | 5.3:+27624126 | MS.gene91909:CDS |
| AGCATTCATTCTCATTCCAG+AGG | 0.606098 | 5.3:-27623647 | None:intergenic |
| TGTTATTCAGCAATCTGATG+AGG | 0.609072 | 5.3:+27623222 | MS.gene91909:CDS |
| GTTTGTCAAGGAGTTCATGA+TGG | 0.609659 | 5.3:+27623172 | MS.gene91909:CDS |
| ACTCCTTGACAAACATTCAA+GGG | 0.609745 | 5.3:-27623163 | None:intergenic |
| CGAGCTAGTAGACGCCATTG+TGG | 0.613296 | 5.3:-27624255 | None:intergenic |
| GAGCCCTTGAATGTTTGTCA+AGG | 0.615186 | 5.3:+27623160 | MS.gene91909:CDS |
| GGTCTTGCATTTAGGGCATG+CGG | 0.616506 | 5.3:+27624147 | MS.gene91909:CDS |
| ATTGGTGGACCTAGGACTCA+TGG | 0.617047 | 5.3:+27624186 | MS.gene91909:CDS |
| AACCCCTACTACTTCGTCCG+GGG | 0.618769 | 5.3:-27623957 | None:intergenic |
| ATAATTTACAAACCAGAACA+TGG | 0.619596 | 5.3:+27623914 | MS.gene91909:CDS |
| CTCAGAATTGTATAGTTCAG+CGG | 0.626222 | 5.3:-27623755 | None:intergenic |
| ACCCCTACTACTTCGTCCGG+GGG | 0.630121 | 5.3:-27623956 | None:intergenic |
| TTATCTACAACGGCAATGAG+AGG | 0.633005 | 5.3:-27624009 | None:intergenic |
| GCAGGTGGTTGTGATTCGGA+GGG | 0.633783 | 5.3:+27623718 | MS.gene91909:CDS |
| TCATTGTTATTGGTGGACCT+AGG | 0.638584 | 5.3:+27624178 | MS.gene91909:CDS |
| GGACCTAGGACTCATGGTGA+AGG | 0.644982 | 5.3:+27624192 | MS.gene91909:CDS |
| TTCCGTGAAATAATCCGAAG+CGG | 0.645763 | 5.3:+27623373 | MS.gene91909:CDS |
| GCTAGTAGACGCCATTGTGG+AGG | 0.646537 | 5.3:-27624252 | None:intergenic |
| GCGGCGAGGTTTATAGATGG+AGG | 0.647194 | 5.3:+27623392 | MS.gene91909:CDS |
| CGTACGACCCAATTCGCCAA+AGG | 0.656928 | 5.3:+27623476 | MS.gene91909:CDS |
| AAAGGTGGATGCATTTGCCG+AGG | 0.658304 | 5.3:+27623494 | MS.gene91909:CDS |
| ATACAATTCATTATCTACAA+CGG | 0.661324 | 5.3:-27624019 | None:intergenic |
| TGTCAAGGAGTTCATGATGG+TGG | 0.663431 | 5.3:+27623175 | MS.gene91909:CDS |
| TATGTTGTAGGTGGAATCGG+CGG | 0.671001 | 5.3:+27623856 | MS.gene91909:CDS |
| CGTACGCTTCCCATTCAAGG+AGG | 0.678294 | 5.3:-27623459 | None:intergenic |
| GTACGCTTCCCATTCAAGGA+GGG | 0.679998 | 5.3:-27623458 | None:intergenic |
| TCTACAACGGCAATGAGAGG+CGG | 0.681780 | 5.3:-27624006 | None:intergenic |
| GGAAGTTAAAAGGTATGACA+AGG | 0.683430 | 5.3:+27624062 | MS.gene91909:CDS |
| CTAATATCGCAATCTCACCA+AGG | 0.689793 | 5.3:-27623696 | None:intergenic |
| CTAGTGAAGGACCTCCACAA+TGG | 0.704380 | 5.3:+27624241 | MS.gene91909:CDS |
| ATGACAAGGAAAGAAAACTG+TGG | 0.706266 | 5.3:+27624076 | MS.gene91909:CDS |
| TCCCGAGCATGAATAAACCG+AGG | 0.713762 | 5.3:+27623797 | MS.gene91909:CDS |
| GATTCAAAGCTTCTTACATG+CGG | 0.719497 | 5.3:+27623883 | MS.gene91909:CDS |
| GAGATTGCGATATTAGCAGG+TGG | 0.734472 | 5.3:+27623703 | MS.gene91909:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACAATTCATTATCTACAA+CGG | - | chr5.3:27624022-27624041 | None:intergenic | 20.0% |
| ! | ACAATTATAAACGAAGTTAC+CGG | - | chr5.3:27624289-27624308 | None:intergenic | 25.0% |
| ! | ATAATTTACAAACCAGAACA+TGG | + | chr5.3:27623914-27623933 | MS.gene91909:CDS | 25.0% |
| ! | TTTCATTGAACTCAATTCTT+GGG | + | chr5.3:27624215-27624234 | MS.gene91909:CDS | 25.0% |
| !! | GATGGAAAATTTTATGTTGT+AGG | + | chr5.3:27623844-27623863 | MS.gene91909:CDS | 25.0% |
| ATCTTGTCACATAGTTGAAA+CGG | + | chr5.3:27623075-27623094 | MS.gene91909:CDS | 30.0% | |
| GATAAAGAATCTTTAGCTGT+AGG | + | chr5.3:27623547-27623566 | MS.gene91909:CDS | 30.0% | |
| GGAAATATGCTCATTGTTAT+TGG | + | chr5.3:27624168-27624187 | MS.gene91909:CDS | 30.0% | |
| GTTTATAATTGTGCTGTGAT+GGG | + | chr5.3:27624297-27624316 | MS.gene91909:CDS | 30.0% | |
| GTTTCATTGAACTCAATTCT+TGG | + | chr5.3:27624214-27624233 | MS.gene91909:CDS | 30.0% | |
| TCAGCAGAATCAATATTCAT+TGG | - | chr5.3:27623016-27623035 | None:intergenic | 30.0% | |
| ! | AATATGCTCATTGTTATTGG+TGG | + | chr5.3:27624171-27624190 | MS.gene91909:CDS | 30.0% |
| ! | ACAGTCTTTTGACAAATTCA+TGG | + | chr5.3:27623623-27623642 | MS.gene91909:CDS | 30.0% |
| ! | GGAAAATTTTATGTTGTAGG+TGG | + | chr5.3:27623847-27623866 | MS.gene91909:CDS | 30.0% |
| ! | TTTTATGTTGTAGGTGGAAT+CGG | + | chr5.3:27623853-27623872 | MS.gene91909:CDS | 30.0% |
| AAACGGTATCATGGAACATT+GGG | + | chr5.3:27623420-27623439 | MS.gene91909:CDS | 35.0% | |
| AACATCTTAGTACACTTCCT+AGG | - | chr5.3:27623052-27623071 | None:intergenic | 35.0% | |
| AACTCCTTGACAAACATTCA+AGG | - | chr5.3:27623167-27623186 | None:intergenic | 35.0% | |
| AATGCTCCAAGATGTTTGTT+CGG | + | chr5.3:27623664-27623683 | MS.gene91909:CDS | 35.0% | |
| ACTCCTTGACAAACATTCAA+GGG | - | chr5.3:27623166-27623185 | None:intergenic | 35.0% | |
| ATGACAAGGAAAGAAAACTG+TGG | + | chr5.3:27624076-27624095 | MS.gene91909:CDS | 35.0% | |
| ATGCTGACATGGAAGTTAAA+AGG | + | chr5.3:27624052-27624071 | MS.gene91909:CDS | 35.0% | |
| CGTTTATAATTGTGCTGTGA+TGG | + | chr5.3:27624296-27624315 | MS.gene91909:CDS | 35.0% | |
| CTCAGAATTGTATAGTTCAG+CGG | - | chr5.3:27623758-27623777 | None:intergenic | 35.0% | |
| CTGGATTTGGACATCAAAAA+TGG | + | chr5.3:27622983-27623002 | MS.gene91909:CDS | 35.0% | |
| GATTCAAAGCTTCTTACATG+CGG | + | chr5.3:27623883-27623902 | MS.gene91909:CDS | 35.0% | |
| GGATTCTGTTCTTTCTCAAA+CGG | + | chr5.3:27623123-27623142 | MS.gene91909:CDS | 35.0% | |
| TAAACGGTATCATGGAACAT+TGG | + | chr5.3:27623419-27623438 | MS.gene91909:CDS | 35.0% | |
| TATAGATGGAGGAGACTAAA+CGG | + | chr5.3:27623403-27623422 | MS.gene91909:CDS | 35.0% | |
| TATTTCGTTGCAACATCCTA+AGG | + | chr5.3:27623102-27623121 | MS.gene91909:CDS | 35.0% | |
| TGAGAAAGAACAGAATCCTT+AGG | - | chr5.3:27623121-27623140 | None:intergenic | 35.0% | |
| TGTTATTCAGCAATCTGATG+AGG | + | chr5.3:27623222-27623241 | MS.gene91909:CDS | 35.0% | |
| ! | AGAAAACTGTGGATTACTGT+TGG | + | chr5.3:27624087-27624106 | MS.gene91909:CDS | 35.0% |
| ! | GGAAGTTAAAAGGTATGACA+AGG | + | chr5.3:27624062-27624081 | MS.gene91909:CDS | 35.0% |
| ! | TAGGAAGTGTACTAAGATGT+TGG | + | chr5.3:27623051-27623070 | MS.gene91909:CDS | 35.0% |
| ACAAACATTCAAGGGCTCAT+TGG | - | chr5.3:27623158-27623177 | None:intergenic | 40.0% | |
| AGCATTCATTCTCATTCCAG+AGG | - | chr5.3:27623650-27623669 | None:intergenic | 40.0% | |
| AGGAATTTCTGTCCATGTTC+TGG | - | chr5.3:27623929-27623948 | None:intergenic | 40.0% | |
| ATTAGCAGGTGGTTGTGATT+CGG | + | chr5.3:27623714-27623733 | MS.gene91909:CDS | 40.0% | |
| CATGAAGTACATGCTGGATT+TGG | + | chr5.3:27622970-27622989 | MS.gene91909:CDS | 40.0% | |
| CTAATATCGCAATCTCACCA+AGG | - | chr5.3:27623699-27623718 | None:intergenic | 40.0% | |
| CTGAAGATGAACATCATCCT+AGG | + | chr5.3:27623032-27623051 | MS.gene91909:CDS | 40.0% | |
| CTTGTGAAACAGAGAACGAT+TGG | + | chr5.3:27622942-27622961 | MS.gene91909:CDS | 40.0% | |
| GAACCGAACTTCTTGTGTTT+GGG | + | chr5.3:27623569-27623588 | MS.gene91909:CDS | 40.0% | |
| GATGTGTTCTGGTGTATTCA+TGG | + | chr5.3:27623822-27623841 | MS.gene91909:CDS | 40.0% | |
| GTCTTACATGAAGTACATGC+TGG | + | chr5.3:27622964-27622983 | MS.gene91909:CDS | 40.0% | |
| GTTTGTCAAGGAGTTCATGA+TGG | + | chr5.3:27623172-27623191 | MS.gene91909:CDS | 40.0% | |
| TGTTCTGGTGTATTCATGGA+TGG | + | chr5.3:27623826-27623845 | MS.gene91909:CDS | 40.0% | |
| TTATCTACAACGGCAATGAG+AGG | - | chr5.3:27624012-27624031 | None:intergenic | 40.0% | |
| TTCCGTGAAATAATCCGAAG+CGG | + | chr5.3:27623373-27623392 | MS.gene91909:CDS | 40.0% | |
| TTCCTCGGTTTATTCATGCT+CGG | - | chr5.3:27623802-27623821 | None:intergenic | 40.0% | |
| TTGACAAATTCATGGTCCTC+TGG | + | chr5.3:27623631-27623650 | MS.gene91909:CDS | 40.0% | |
| ! | TCATTGTTATTGGTGGACCT+AGG | + | chr5.3:27624178-27624197 | MS.gene91909:CDS | 40.0% |
| !!! | ATTCTGGTTCTCTTTTACCG+AGG | + | chr5.3:27623269-27623288 | MS.gene91909:CDS | 40.0% |
| AAACCGAGGAAGATGTGTTC+TGG | + | chr5.3:27623811-27623830 | MS.gene91909:CDS | 45.0% | |
| AAACCTTCACCATGAGTCCT+AGG | - | chr5.3:27624198-27624217 | None:intergenic | 45.0% | |
| AATTCTTGGGTGCCTAGTGA+AGG | + | chr5.3:27624228-27624247 | MS.gene91909:CDS | 45.0% | |
| ACACCAGAACACATCTTCCT+CGG | - | chr5.3:27623817-27623836 | None:intergenic | 45.0% | |
| AGCGATGTCGATGAATGGTT+GGG | + | chr5.3:27624125-27624144 | MS.gene91909:CDS | 45.0% | |
| ATCTATAAACCTCGCCGCTT+CGG | - | chr5.3:27623390-27623409 | None:intergenic | 45.0% | |
| ATGGTTGGGGTCTTGCATTT+AGG | + | chr5.3:27624139-27624158 | MS.gene91909:CDS | 45.0% | |
| CCTCCCAAACACAAGAAGTT+CGG | - | chr5.3:27623575-27623594 | None:intergenic | 45.0% | |
| CGAACTTCTTGTGTTTGGGA+GGG | + | chr5.3:27623573-27623592 | MS.gene91909:CDS | 45.0% | |
| CGCTGTTCAAGGTCTGATTA+TGG | + | chr5.3:27623325-27623344 | MS.gene91909:CDS | 45.0% | |
| CTACTAGCTCGAAAACGATC+CGG | + | chr5.3:27624267-27624286 | MS.gene91909:CDS | 45.0% | |
| GAGATTGCGATATTAGCAGG+TGG | + | chr5.3:27623703-27623722 | MS.gene91909:CDS | 45.0% | |
| GAGCCCTTGAATGTTTGTCA+AGG | + | chr5.3:27623160-27623179 | MS.gene91909:CDS | 45.0% | |
| GAGGAGACTAAACGGTATCA+TGG | + | chr5.3:27623411-27623430 | MS.gene91909:CDS | 45.0% | |
| GGAACCGAACTTCTTGTGTT+TGG | + | chr5.3:27623568-27623587 | MS.gene91909:CDS | 45.0% | |
| GGTGAGATTGCGATATTAGC+AGG | + | chr5.3:27623700-27623719 | MS.gene91909:CDS | 45.0% | |
| TATGTTGTAGGTGGAATCGG+CGG | + | chr5.3:27623856-27623875 | MS.gene91909:CDS | 45.0% | |
| TCCTCGGTTTATTCATGCTC+GGG | - | chr5.3:27623801-27623820 | None:intergenic | 45.0% | |
| TGGTTGGGGTCTTGCATTTA+GGG | + | chr5.3:27624140-27624159 | MS.gene91909:CDS | 45.0% | |
| TGTCAAGGAGTTCATGATGG+TGG | + | chr5.3:27623175-27623194 | MS.gene91909:CDS | 45.0% | |
| ! | CAATCGTTTGAAGCCATCCT+CGG | - | chr5.3:27623514-27623533 | None:intergenic | 45.0% |
| ! | TGCTGCTGATTATGCTGACA+TGG | + | chr5.3:27624041-27624060 | MS.gene91909:CDS | 45.0% |
| AAAGGTGGATGCATTTGCCG+AGG | + | chr5.3:27623494-27623513 | MS.gene91909:CDS | 50.0% | |
| AGAACCCCTACTACTTCGTC+CGG | - | chr5.3:27623962-27623981 | None:intergenic | 50.0% | |
| CATTGTGGAGGTCCTTCACT+AGG | - | chr5.3:27624243-27624262 | None:intergenic | 50.0% | |
| CCGAACTTCTTGTGTTTGGG+AGG | + | chr5.3:27623572-27623591 | MS.gene91909:CDS | 50.0% | |
| CGCCGCTTCGGATTATTTCA+CGG | - | chr5.3:27623378-27623397 | None:intergenic | 50.0% | |
| CTAGTGAAGGACCTCCACAA+TGG | + | chr5.3:27624241-27624260 | MS.gene91909:CDS | 50.0% | |
| GAAATTCCTGACATGTCCCC+CGG | + | chr5.3:27623940-27623959 | MS.gene91909:CDS | 50.0% | |
| GAACGAGCGATGTCGATGAA+TGG | + | chr5.3:27624120-27624139 | MS.gene91909:CDS | 50.0% | |
| GAAGCGGCGAGGTTTATAGA+TGG | + | chr5.3:27623389-27623408 | MS.gene91909:CDS | 50.0% | |
| GACACACGATTGAGCTGTCT+CGG | - | chr5.3:27623300-27623319 | None:intergenic | 50.0% | |
| GAGCGATGTCGATGAATGGT+TGG | + | chr5.3:27624124-27624143 | MS.gene91909:CDS | 50.0% | |
| GCAGAGCCGAACAAACATCT+TGG | - | chr5.3:27623673-27623692 | None:intergenic | 50.0% | |
| GCATCCACCTTTGGCGAATT+GGG | - | chr5.3:27623486-27623505 | None:intergenic | 50.0% | |
| GCGATGTCGATGAATGGTTG+GGG | + | chr5.3:27624126-27624145 | MS.gene91909:CDS | 50.0% | |
| GTACGCTTCCCATTCAAGGA+GGG | - | chr5.3:27623461-27623480 | None:intergenic | 50.0% | |
| TCCCGAGCATGAATAAACCG+AGG | + | chr5.3:27623797-27623816 | MS.gene91909:CDS | 50.0% | |
| TCGGCAAATGCATCCACCTT+TGG | - | chr5.3:27623495-27623514 | None:intergenic | 50.0% | |
| TCTACAACGGCAATGAGAGG+CGG | - | chr5.3:27624009-27624028 | None:intergenic | 50.0% | |
| TGAAATAATCCGAAGCGGCG+AGG | + | chr5.3:27623378-27623397 | MS.gene91909:CDS | 50.0% | |
| TGCATCCACCTTTGGCGAAT+TGG | - | chr5.3:27623487-27623506 | None:intergenic | 50.0% | |
| TGTGTCTCAGTCGCTGTTCA+AGG | + | chr5.3:27623314-27623333 | MS.gene91909:CDS | 50.0% | |
| TTCATCGACATCGCTCGTTC+TGG | - | chr5.3:27624120-27624139 | None:intergenic | 50.0% | |
| ! | ATTGGTGGACCTAGGACTCA+TGG | + | chr5.3:27624186-27624205 | MS.gene91909:CDS | 50.0% |
| ! | GGTCTTGCATTTAGGGCATG+CGG | + | chr5.3:27624147-27624166 | MS.gene91909:CDS | 50.0% |
| !! | GTCGTTGCTGTGTTGTTTCG+AGG | + | chr5.3:27622906-27622925 | MS.gene91909:CDS | 50.0% |
| AACCCCTACTACTTCGTCCG+GGG | - | chr5.3:27623960-27623979 | None:intergenic | 55.0% | |
| ACGACCCAATTCGCCAAAGG+TGG | + | chr5.3:27623479-27623498 | MS.gene91909:CDS | 55.0% | |
| CGAGCTAGTAGACGCCATTG+TGG | - | chr5.3:27624258-27624277 | None:intergenic | 55.0% | |
| CGTACGACCCAATTCGCCAA+AGG | + | chr5.3:27623476-27623495 | MS.gene91909:CDS | 55.0% | |
| CGTACGCTTCCCATTCAAGG+AGG | - | chr5.3:27623462-27623481 | None:intergenic | 55.0% | |
| CTCGTGTGCCCTCCTTGAAT+GGG | + | chr5.3:27623450-27623469 | MS.gene91909:CDS | 55.0% | |
| GAACCCCTACTACTTCGTCC+GGG | - | chr5.3:27623961-27623980 | None:intergenic | 55.0% | |
| GAGCTGTCTCGGTTCATCCT+CGG | - | chr5.3:27623289-27623308 | None:intergenic | 55.0% | |
| GCAGCATGCTGGAGATTCGT+CGG | + | chr5.3:27623246-27623265 | MS.gene91909:CDS | 55.0% | |
| GCGGCGAGGTTTATAGATGG+AGG | + | chr5.3:27623392-27623411 | MS.gene91909:CDS | 55.0% | |
| GCTAGTAGACGCCATTGTGG+AGG | - | chr5.3:27624255-27624274 | None:intergenic | 55.0% | |
| GCTGGAGATTCGTCGGATTC+TGG | + | chr5.3:27623253-27623272 | MS.gene91909:CDS | 55.0% | |
| GGACCTAGGACTCATGGTGA+AGG | + | chr5.3:27624192-27624211 | MS.gene91909:CDS | 55.0% | |
| GGTCGTACGCTTCCCATTCA+AGG | - | chr5.3:27623465-27623484 | None:intergenic | 55.0% | |
| GTGGATGCATTTGCCGAGGA+TGG | + | chr5.3:27623498-27623517 | MS.gene91909:CDS | 55.0% | |
| TCTCGTGTGCCCTCCTTGAA+TGG | + | chr5.3:27623449-27623468 | MS.gene91909:CDS | 55.0% | |
| TCTGATGAGGAGCAGCATGC+TGG | + | chr5.3:27623235-27623254 | MS.gene91909:CDS | 55.0% | |
| TTGTTCGGCTCTGCAAGCCT+TGG | + | chr5.3:27623679-27623698 | MS.gene91909:CDS | 55.0% | |
| ! | AGCAGGTGGTTGTGATTCGG+AGG | + | chr5.3:27623717-27623736 | MS.gene91909:CDS | 55.0% |
| ! | GCAGGTGGTTGTGATTCGGA+GGG | + | chr5.3:27623718-27623737 | MS.gene91909:CDS | 55.0% |
| ACCCCTACTACTTCGTCCGG+GGG | - | chr5.3:27623959-27623978 | None:intergenic | 60.0% | |
| GTCCCCCGGACGAAGTAGTA+GGG | + | chr5.3:27623954-27623973 | MS.gene91909:CDS | 60.0% | |
| TCCCCCGGACGAAGTAGTAG+GGG | + | chr5.3:27623955-27623974 | MS.gene91909:CDS | 60.0% | |
| TGAGATGCCTGCTGCAACCG+AGG | + | chr5.3:27623981-27624000 | MS.gene91909:CDS | 60.0% | |
| TGTCCCCCGGACGAAGTAGT+AGG | + | chr5.3:27623953-27623972 | MS.gene91909:CDS | 60.0% | |
| CTTCGTCCGGGGGACATGTC+AGG | - | chr5.3:27623949-27623968 | None:intergenic | 65.0% | |
| GGCAATGAGAGGCGGTGCCT+CGG | - | chr5.3:27624001-27624020 | None:intergenic | 65.0% | |
| GGCGGTGCCTCGGTTGCAGC+AGG | - | chr5.3:27623991-27624010 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 27622896 | 27624326 | 27622896 | ID=MS.gene91909 |
| chr5.3 | mRNA | 27622896 | 27624326 | 27622896 | ID=MS.gene91909.t1;Parent=MS.gene91909 |
| chr5.3 | exon | 27622896 | 27624326 | 27622896 | ID=MS.gene91909.t1.exon1;Parent=MS.gene91909.t1 |
| chr5.3 | CDS | 27622896 | 27624326 | 27622896 | ID=cds.MS.gene91909.t1;Parent=MS.gene91909.t1 |
| Gene Sequence |
| Protein sequence |
