AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25443

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25443.t1 MTR_8g468030 98.390 497 8 0 1 497 1 497 0.0 1020
MS.gene25443.t1 MTR_5g039390 72.745 510 92 7 1 497 1 476 0.0 742
MS.gene25443.t1 MTR_4g108280 62.803 371 135 2 130 497 10 380 2.11e-175 498
MS.gene25443.t1 MTR_0345s0010 62.323 353 132 1 145 497 84 435 5.21e-164 471
MS.gene25443.t1 MTR_0105s0170 62.323 353 132 1 145 497 97 448 3.75e-163 469
MS.gene25443.t1 MTR_0105s0130 62.323 353 132 1 145 497 84 435 4.92e-163 469
MS.gene25443.t1 MTR_0105s0040 62.323 353 132 1 145 497 182 533 2.60e-162 471
MS.gene25443.t1 MTR_0105s0020 61.473 353 135 1 145 497 84 435 2.63e-162 467
MS.gene25443.t1 MTR_4g022260 62.890 353 130 1 145 497 66 417 8.84e-162 465
MS.gene25443.t1 MTR_0496s0010 61.756 353 128 2 145 497 66 411 3.66e-160 461
MS.gene25443.t1 MTR_1330s0010 61.960 347 131 1 145 491 97 442 4.49e-159 459
MS.gene25443.t1 MTR_4g022400 60.169 354 140 1 144 497 62 414 3.41e-154 445
MS.gene25443.t1 MTR_2g094093 51.130 354 160 4 149 497 19 364 4.77e-117 349
MS.gene25443.t1 MTR_7g029290 36.031 383 217 10 122 495 38 401 2.35e-67 222
MS.gene25443.t1 MTR_6g023805 37.466 363 193 8 151 495 57 403 5.11e-64 213
MS.gene25443.t1 MTR_0105s0060 62.637 91 34 0 407 497 15 105 1.67e-36 130
MS.gene25443.t1 MTR_3g085570 27.306 271 168 8 124 373 5 267 2.38e-21 95.9
MS.gene25443.t1 MTR_8g080370 25.424 295 182 9 102 373 4 283 1.67e-16 81.6
MS.gene25443.t1 MTR_3g073640 24.642 349 187 12 150 429 43 384 5.21e-16 80.9
MS.gene25443.t1 MTR_4g114110 25.890 309 198 11 126 422 58 347 8.89e-16 79.7
MS.gene25443.t1 MTR_8g085600 26.056 284 182 9 150 422 77 343 1.12e-15 79.7
MS.gene25443.t1 MTR_2g008440 24.438 356 190 11 146 429 39 387 1.21e-14 76.6
MS.gene25443.t1 MTR_4g011900 22.255 337 220 7 150 460 5 325 1.34e-14 75.5
MS.gene25443.t1 MTR_7g095220 21.605 324 207 7 148 454 2 295 1.44e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25443.t1 AT2G02870 58.268 508 160 11 1 497 1 467 0.0 553
MS.gene25443.t1 AT2G02870 58.268 508 160 11 1 497 1 467 0.0 553
MS.gene25443.t1 AT2G02870 58.268 508 160 11 1 497 1 467 0.0 553
MS.gene25443.t1 AT1G14330 69.841 378 108 3 123 497 67 441 0.0 541
MS.gene25443.t1 AT1G26930 67.935 368 115 1 133 497 54 421 0.0 525
MS.gene25443.t1 AT1G74510 65.169 356 115 2 151 497 96 451 7.99e-169 484
MS.gene25443.t1 AT1G74510 65.169 356 115 2 151 497 96 451 7.99e-169 484
MS.gene25443.t1 AT1G74510 65.169 356 115 2 151 497 96 451 7.99e-169 484
MS.gene25443.t1 AT1G26930 57.609 368 101 2 133 497 54 369 1.40e-146 424
MS.gene25443.t1 AT5G60570 47.297 370 180 6 134 497 33 393 2.95e-109 330
MS.gene25443.t1 AT5G60570 47.297 370 180 6 134 497 33 393 2.95e-109 330
MS.gene25443.t1 AT5G60570 47.297 370 180 6 134 497 33 393 2.95e-109 330
MS.gene25443.t1 AT3G27150 35.655 359 208 9 151 495 71 420 1.25e-63 213
MS.gene25443.t1 AT3G27150 35.655 359 208 9 151 495 71 420 1.25e-63 213
MS.gene25443.t1 AT5G40680 33.611 360 222 6 146 495 61 413 3.95e-59 201
MS.gene25443.t1 AT1G16250 24.024 333 219 8 145 454 3 324 4.57e-22 98.2
MS.gene25443.t1 AT1G16250 24.024 333 219 8 145 454 3 324 4.57e-22 98.2
MS.gene25443.t1 AT1G67480 28.151 238 152 5 150 373 39 271 1.38e-20 94.0
MS.gene25443.t1 AT1G67480 28.151 238 152 5 150 373 39 271 1.38e-20 94.0
MS.gene25443.t1 AT1G67480 28.151 238 152 5 150 373 39 271 1.38e-20 94.0
MS.gene25443.t1 AT1G15670 25.937 347 208 13 150 466 3 330 2.34e-17 84.0
MS.gene25443.t1 AT1G55270 25.352 284 184 10 150 422 78 344 1.13e-15 79.7
MS.gene25443.t1 AT1G80440 24.924 329 211 11 150 457 3 316 1.07e-13 72.8
MS.gene25443.t1 AT3G63220 23.832 214 145 4 148 344 2 214 2.56e-13 71.6
MS.gene25443.t1 AT3G63220 23.832 214 145 4 148 344 9 221 2.90e-13 71.6
MS.gene25443.t1 AT3G63220 23.832 214 145 4 148 344 9 221 2.90e-13 71.6

Find 144 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAATTCATGCCGGATT+TGG 0.208968 8.2:-40862551 MS.gene25443:CDS
GGAGATAGGCTTATTGTTAT+TGG 0.237398 8.2:-40861296 MS.gene25443:CDS
TTCCTGGGCTTGATCATGTT+TGG 0.245172 8.2:+40861665 None:intergenic
ACGGTTGGGGTCTTGCATTT+AGG 0.270827 8.2:-40861325 MS.gene25443:CDS
TCCTGGGCTTGATCATGTTT+GGG 0.274266 8.2:+40861666 None:intergenic
CGGTTGGGGTCTTGCATTTA+GGG 0.286736 8.2:-40861324 MS.gene25443:CDS
TGCATCCACCGTTCGCGAAT+TGG 0.289308 8.2:+40861980 None:intergenic
TTGACAAACTCGTGGGAATC+TGG 0.303822 8.2:-40861833 MS.gene25443:CDS
GGTACCGAACTTCTTGTGTT+TGG 0.310632 8.2:-40861896 MS.gene25443:CDS
CGTTGTTCGAGGTCCGATTA+TGG 0.329116 8.2:-40862139 MS.gene25443:CDS
TTTCCTGTGCCCTCCTTGAA+TGG 0.329821 8.2:-40862015 MS.gene25443:CDS
CATTCGTTGGAAGCCATTCT+AGG 0.330901 8.2:+40861953 None:intergenic
TACCCCAGAGCACATCTTCC+TGG 0.332847 8.2:+40861649 None:intergenic
ACGATAAAGGTAACAAAGTA+TGG 0.334348 8.2:-40861388 MS.gene25443:CDS
CATTTAGGGCATGTGGAGAT+AGG 0.347335 8.2:-40861310 MS.gene25443:CDS
GTACCGAACTTCTTGTGTTT+GGG 0.354894 8.2:-40861895 MS.gene25443:CDS
CGCAGCCGAGGAAGGTTAAC+AGG 0.363634 8.2:-40862486 MS.gene25443:CDS
CCGTTCGCGAATTGGATCAT+AGG 0.379832 8.2:+40861988 None:intergenic
TGTTTCCCATGTTTGATTCT+CGG 0.387447 8.2:+40861691 None:intergenic
TTCCTGTGCCCTCCTTGAAT+GGG 0.390967 8.2:-40862014 MS.gene25443:CDS
GCTTACAATTGGTAGATTAC+CGG 0.406813 8.2:-40861366 MS.gene25443:CDS
AGAGCTTCCGCAACATAATT+CGG 0.411413 8.2:-40862096 MS.gene25443:CDS
CAACGAATGCTTCATGTGTT+CGG 0.415249 8.2:-40861939 MS.gene25443:CDS
CGAGGCAAGTGAACCATAAT+CGG 0.416718 8.2:+40862126 None:intergenic
GATGTGCTCTGGGGTATTCA+TGG 0.419067 8.2:-40861642 MS.gene25443:CDS
CAGAAGAAGCAGCTTGATGA+TGG 0.421720 8.2:-40862286 MS.gene25443:CDS
TGTCACCCGGGCGTAGTGCT+AGG 0.424613 8.2:-40861511 MS.gene25443:CDS
CTACGCCCGGGTGACATGTT+AGG 0.424620 8.2:+40861518 None:intergenic
CCTGTCTAAGCCGTTGTTCG+AGG 0.429093 8.2:-40862150 MS.gene25443:CDS
AATCTCCTGTTAACCTTCCT+CGG 0.429428 8.2:+40862481 None:intergenic
TGTTGTTCCGCAAATCGATC+TGG 0.429697 8.2:-40862428 MS.gene25443:CDS
AGGCAGAGGCAGAGAACAAT+TGG 0.435851 8.2:-40862579 MS.gene25443:CDS
ATGCTTCATGTGTTCGGATA+AGG 0.437733 8.2:-40861933 MS.gene25443:CDS
GTGGATGCATTTACCTAGAA+TGG 0.438443 8.2:-40861966 MS.gene25443:CDS
TTCATCGACGCCGCTCTCTC+CGG 0.439593 8.2:+40861347 None:intergenic
GCAATTATAAACAAAGTTAC+CGG 0.440298 8.2:+40861178 None:intergenic
GGTGGTGCCTCGGCTGTGGC+AGG 0.444795 8.2:+40861476 None:intergenic
AGCGGCGTCGATGAACGGTT+GGG 0.449670 8.2:-40861339 MS.gene25443:CDS
AAGCCCAGGAAGATGTGCTC+TGG 0.455338 8.2:-40861653 MS.gene25443:CDS
GCACTTTGGCAATCATTCAA+TGG 0.457578 8.2:+40862379 None:intergenic
TTCTAGTTCTTGTCAGGCAG+AGG 0.462830 8.2:-40862593 MS.gene25443:CDS
GTCACCCGGGCGTAGTGCTA+GGG 0.467230 8.2:-40861510 MS.gene25443:CDS
TGTTTATAATTGCGCCGTGA+TGG 0.469236 8.2:-40861168 MS.gene25443:CDS
AACAAAGTATGGCTTACAAT+TGG 0.470901 8.2:-40861377 MS.gene25443:CDS
GTTGGAAGGTCGTTCCTGCT+TGG 0.471319 8.2:-40862629 MS.gene25443:CDS
TAGAGGTGGTGCCTCGGCTG+TGG 0.471382 8.2:+40861472 None:intergenic
GATCATAGGCCTCCCATTCA+AGG 0.478811 8.2:+40862002 None:intergenic
GCAGAACCAAACAAGCATCT+TGG 0.482493 8.2:+40861794 None:intergenic
AGATTGGAATCTTGTTATCA+AGG 0.484042 8.2:-40862463 MS.gene25443:CDS
AAACCCTCGCCATGACTCCT+TGG 0.487015 8.2:+40861269 None:intergenic
GAGGAAGGTTAACAGGAGAT+TGG 0.488758 8.2:-40862479 MS.gene25443:CDS
GTTTATAATTGCGCCGTGAT+GGG 0.493022 8.2:-40861167 MS.gene25443:CDS
CTCGCCGCACGTAAGAAGCT+TGG 0.497088 8.2:+40861577 None:intergenic
CCTCGAACAACGGCTTAGAC+AGG 0.497131 8.2:+40862150 None:intergenic
GTCGTTCCTGCTTGGTTCCG+AGG 0.503606 8.2:-40862621 MS.gene25443:CDS
TGCTGCTTCTGTTCATCCGT+CGG 0.504235 8.2:+40862238 None:intergenic
AGCCCAGGAAGATGTGCTCT+GGG 0.511106 8.2:-40861652 MS.gene25443:CDS
CTGTTAACCTTCCTCGGCTG+CGG 0.511143 8.2:+40862487 None:intergenic
GATAGGCTTATTGTTATTGG+CGG 0.511196 8.2:-40861293 MS.gene25443:CDS
GGTCTTGCATTTAGGGCATG+TGG 0.511219 8.2:-40861317 MS.gene25443:CDS
GTCTTTCATGAAATTCATGC+CGG 0.514207 8.2:-40862557 MS.gene25443:CDS
AACAGAAGCAGCATCATGTA+GGG 0.519682 8.2:-40862228 MS.gene25443:CDS
CTGTGCCCTCCTTGAATGGG+AGG 0.521239 8.2:-40862011 MS.gene25443:CDS
GAGCGGCGTCGATGAACGGT+TGG 0.522706 8.2:-40861340 MS.gene25443:CDS
CGATCCCCTAGCACTACGCC+CGG 0.523209 8.2:+40861505 None:intergenic
GCGGCGTCGATGAACGGTTG+GGG 0.523734 8.2:-40861338 MS.gene25443:CDS
CCGAACTTCTTGTGTTTGGG+AGG 0.526756 8.2:-40861892 MS.gene25443:CDS
CATTGTGGAGGTCCTTCACT+AGG 0.527917 8.2:+40861224 None:intergenic
GACTGTATCTGTATATAACA+TGG 0.528568 8.2:+40861858 None:intergenic
AGAGAGATTGCAATTCTAGC+TGG 0.531625 8.2:-40861764 MS.gene25443:CDS
CGCCGCTGATTATGCTGACA+TGG 0.534149 8.2:-40861423 MS.gene25443:CDS
ACCCCAGAGCACATCTTCCT+GGG 0.535814 8.2:+40861650 None:intergenic
GAACAGAAGCAGCATCATGT+AGG 0.538092 8.2:-40862229 MS.gene25443:CDS
CCTCCCAAACACAAGAAGTT+CGG 0.539106 8.2:+40861892 None:intergenic
CGGGCGTAGTGCTAGGGGAT+CGG 0.539496 8.2:-40861504 MS.gene25443:CDS
TTCATCCGTCGGCTCGTCAG+TGG 0.539654 8.2:+40862249 None:intergenic
CTACTTGCTAGAAAACAATC+CGG 0.542893 8.2:-40861197 MS.gene25443:CDS
CTCCCATTCAAGGAGGGCAC+AGG 0.543252 8.2:+40862012 None:intergenic
TCATCATCCAGATCGATTTG+CGG 0.545355 8.2:+40862421 None:intergenic
ATCTTGTTATCAAGGTGAGA+TGG 0.548591 8.2:-40862455 MS.gene25443:CDS
CATAATCGGACCTCGAACAA+CGG 0.554013 8.2:+40862140 None:intergenic
CGAACTTCTTGTGTTTGGGA+GGG 0.560794 8.2:-40861891 MS.gene25443:CDS
TGGTAGATTACCGGAGAGAG+CGG 0.564163 8.2:-40861357 MS.gene25443:CDS
TATCGTTGGCGAAGACTGAA+CGG 0.565024 8.2:-40862061 MS.gene25443:CDS
CGAACACATGAAGCATTCGT+TGG 0.566259 8.2:+40861940 None:intergenic
TATAGAGCTCAGCCGAGTCA+AGG 0.569597 8.2:+40861717 None:intergenic
TAATTCCACTGACGAGCCGA+CGG 0.571828 8.2:-40862254 MS.gene25443:CDS
TGCTCTGGGGTATTCATGGA+CGG 0.572915 8.2:-40861638 MS.gene25443:CDS
GAGAGAGCGGCGTCGATGAA+CGG 0.573082 8.2:-40861344 MS.gene25443:CDS
GATAAGGAGTCTTTAGCGGT+AGG 0.574089 8.2:-40861917 MS.gene25443:CDS
GCTGGTGGTTGTGATTCAGA+TGG 0.575080 8.2:-40861746 MS.gene25443:CDS
GGAGATGCCTGCCACAGCCG+AGG 0.577171 8.2:-40861483 MS.gene25443:CDS
TTCGGATAAGGAGTCTTTAG+CGG 0.577829 8.2:-40861921 MS.gene25443:CDS
ATGATCCAATTCGCGAACGG+TGG 0.578353 8.2:-40861985 MS.gene25443:CDS
AAACAACCTCGGAACCAAGC+AGG 0.585898 8.2:+40862615 None:intergenic
GAGATTGCAATTCTAGCTGG+TGG 0.586483 8.2:-40861761 MS.gene25443:CDS
ATGAAGATGAACCGCAGCCG+AGG 0.586920 8.2:-40862498 MS.gene25443:CDS
ATTGGCGGACCAAGGAGTCA+TGG 0.588019 8.2:-40861278 MS.gene25443:CDS
AGCAACTAGAGGTGGTGCCT+CGG 0.588296 8.2:+40861466 None:intergenic
GAAATTCCTAACATGTCACC+CGG 0.589237 8.2:-40861524 MS.gene25443:CDS
CAAGCTTCTTACGTGCGGCG+AGG 0.604569 8.2:-40861576 MS.gene25443:CDS
TCCCAAACATGATCAAGCCC+AGG 0.608139 8.2:-40861667 MS.gene25443:CDS
AGATGAACCGCAGCCGAGGA+AGG 0.608999 8.2:-40862494 MS.gene25443:CDS
CTGGTGGTTGTGATTCAGAT+GGG 0.609342 8.2:-40861745 MS.gene25443:CDS
AGAAGAAGCAGCTTGATGAT+GGG 0.610932 8.2:-40862285 MS.gene25443:CDS
TCACCCGGGCGTAGTGCTAG+GGG 0.612909 8.2:-40861509 MS.gene25443:CDS
ACTCGTGGGAATCTGGAATG+AGG 0.616994 8.2:-40861826 MS.gene25443:CDS
AGATGGGCATATCCTTGACT+CGG 0.619344 8.2:-40861729 MS.gene25443:CDS
GGACCAAGGAGTCATGGCGA+GGG 0.620296 8.2:-40861272 MS.gene25443:CDS
CGGACCAAGGAGTCATGGCG+AGG 0.624374 8.2:-40861273 MS.gene25443:CDS
AATTCTTCAGCATCCCATCA+CGG 0.624527 8.2:+40861154 None:intergenic
AATTCATGGGTTCCTAGTGA+AGG 0.624631 8.2:-40861236 MS.gene25443:CDS
TTTACAACAGCAACTAGAGG+TGG 0.625889 8.2:+40861458 None:intergenic
TCTGGATGATGATCAGAGGA+AGG 0.626736 8.2:-40862410 MS.gene25443:CDS
ACTGTATCTGTATATAACAT+GGG 0.627241 8.2:+40861859 None:intergenic
TGAACGGTATCATAGAGCAC+TGG 0.630163 8.2:-40862045 MS.gene25443:CDS
CACCGCTCCGAATTATGTTG+CGG 0.630361 8.2:+40862089 None:intergenic
TTATTGTTATTGGCGGACCA+AGG 0.631861 8.2:-40861286 MS.gene25443:CDS
ATAACTCCGAGAATCAAACA+TGG 0.632851 8.2:-40861697 MS.gene25443:CDS
GATCCCCTAGCACTACGCCC+GGG 0.636164 8.2:+40861506 None:intergenic
ACAATCTACAAACTAGAACA+TGG 0.636640 8.2:-40861550 MS.gene25443:CDS
GCAAGTAGATTCCATTGTGG+AGG 0.637868 8.2:+40861212 None:intergenic
TTATTTACAACAGCAACTAG+AGG 0.638870 8.2:+40861455 None:intergenic
TCGATCTGGATGATGATCAG+AGG 0.644226 8.2:-40862414 MS.gene25443:CDS
ATTGAATGATTGCCAAAGTG+CGG 0.655209 8.2:-40862377 MS.gene25443:CDS
CGCTGATTATGCTGACATGG+AGG 0.655991 8.2:-40861420 MS.gene25443:CDS
CTCCATGTCAGCATAATCAG+CGG 0.662179 8.2:+40861421 None:intergenic
CCTATGATCCAATTCGCGAA+CGG 0.662545 8.2:-40861988 MS.gene25443:CDS
GGAGCGGTGAGCTCTATCGT+TGG 0.662547 8.2:-40862075 MS.gene25443:CDS
TAACTCCGAGAATCAAACAT+GGG 0.666523 8.2:-40861696 MS.gene25443:CDS
CTAGAATTGCAATCTCTCTG+AGG 0.668609 8.2:+40861768 None:intergenic
CTAGTGAAGGACCTCCACAA+TGG 0.672606 8.2:-40861223 MS.gene25443:CDS
CTAGCAAGTAGATTCCATTG+TGG 0.677748 8.2:+40861209 None:intergenic
ATAGGCCTCCCATTCAAGGA+GGG 0.678331 8.2:+40862006 None:intergenic
GCTGCAAAGACTATGTTGGA+AGG 0.683095 8.2:-40862643 None:intergenic
CAAGAACTAGAAAACAACCT+CGG 0.684499 8.2:+40862604 None:intergenic
CATAGGCCTCCCATTCAAGG+AGG 0.690418 8.2:+40862005 None:intergenic
GATTCCAAGCTTCTTACGTG+CGG 0.696878 8.2:-40861581 MS.gene25443:CDS
AAATTCCTAACATGTCACCC+GGG 0.697406 8.2:-40861523 MS.gene25443:CDS
ACAGAAGCAGCATCATGTAG+GGG 0.701644 8.2:-40862227 MS.gene25443:CDS
GAGCTGTCCCGATTCATGCG+CGG 0.706639 8.2:+40862178 None:intergenic
GCCCAGGAAGATGTGCTCTG+GGG 0.715567 8.2:-40861651 MS.gene25443:CDS
TTCCGCAACATAATTCGGAG+CGG 0.727088 8.2:-40862091 MS.gene25443:CDS
GCGGAAGCTCTGATTCAGCG+AGG 0.753966 8.2:+40862108 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTGAACTCAATTCAT+GGG - chr8.2:40862542-40862561 MS.gene25443:CDS 20.0%
! ACTGTATCTGTATATAACAT+GGG + chr8.2:40861935-40861954 None:intergenic 25.0%
! GCAATTATAAACAAAGTTAC+CGG + chr8.2:40862616-40862635 None:intergenic 25.0%
!! ACGGAAATTTTTATGTTATC+GGG - chr8.2:40862172-40862191 MS.gene25443:CDS 25.0%
!! GACGGAAATTTTTATGTTAT+CGG - chr8.2:40862171-40862190 MS.gene25443:CDS 25.0%
!!! GTTTTATTGAACTCAATTCA+TGG - chr8.2:40862541-40862560 MS.gene25443:CDS 25.0%
AACAAAGTATGGCTTACAAT+TGG - chr8.2:40862414-40862433 MS.gene25443:CDS 30.0%
ACAATCTACAAACTAGAACA+TGG - chr8.2:40862241-40862260 MS.gene25443:CDS 30.0%
ACGATAAAGGTAACAAAGTA+TGG - chr8.2:40862403-40862422 MS.gene25443:CDS 30.0%
CCGGATTTGGAAATAAAAAA+CGG - chr8.2:40861253-40861272 MS.gene25443:CDS 30.0%
GACTGTATCTGTATATAACA+TGG + chr8.2:40861936-40861955 None:intergenic 30.0%
TTATTTACAACAGCAACTAG+AGG + chr8.2:40862339-40862358 None:intergenic 30.0%
! AATTTTCCAAGATGCTTGTT+TGG - chr8.2:40861991-40862010 MS.gene25443:CDS 30.0%
! AGATTGGAATCTTGTTATCA+AGG - chr8.2:40861328-40861347 MS.gene25443:CDS 30.0%
! CGGAAATTTTTATGTTATCG+GGG - chr8.2:40862173-40862192 MS.gene25443:CDS 30.0%
! GAGGTTAAAAAGTACGATAA+AGG - chr8.2:40862390-40862409 MS.gene25443:CDS 30.0%
! GGAAATTTTTATGTTATCGG+GGG - chr8.2:40862174-40862193 MS.gene25443:CDS 30.0%
!! TTTTTCAGCCAAAAACATGA+CGG + chr8.2:40861458-40861477 None:intergenic 30.0%
!!! CCGTTTTTTATTTCCAAATC+CGG + chr8.2:40861256-40861275 None:intergenic 30.0%
ATAACTCCGAGAATCAAACA+TGG - chr8.2:40862094-40862113 MS.gene25443:CDS 35.0%
ATTGAATGATTGCCAAAGTG+CGG - chr8.2:40861414-40861433 MS.gene25443:CDS 35.0%
CAAGAACTAGAAAACAACCT+CGG + chr8.2:40861190-40861209 None:intergenic 35.0%
GAAATAAAAAACGGAAAGCG+AGG - chr8.2:40861262-40861281 MS.gene25443:CDS 35.0%
GCTTACAATTGGTAGATTAC+CGG - chr8.2:40862425-40862444 MS.gene25443:CDS 35.0%
GGAGATAGGCTTATTGTTAT+TGG - chr8.2:40862495-40862514 MS.gene25443:CDS 35.0%
GTCTTTCATGAAATTCATGC+CGG - chr8.2:40861234-40861253 MS.gene25443:CDS 35.0%
TAACTCCGAGAATCAAACAT+GGG - chr8.2:40862095-40862114 MS.gene25443:CDS 35.0%
TGTTTCCCATGTTTGATTCT+CGG + chr8.2:40862103-40862122 None:intergenic 35.0%
! ATCTTGTTATCAAGGTGAGA+TGG - chr8.2:40861336-40861355 MS.gene25443:CDS 35.0%
! CTACTTGCTAGAAAACAATC+CGG - chr8.2:40862594-40862613 MS.gene25443:CDS 35.0%
! GATAGGCTTATTGTTATTGG+CGG - chr8.2:40862498-40862517 MS.gene25443:CDS 35.0%
! TTTTATGTTATCGGGGGAAT+TGG - chr8.2:40862180-40862199 MS.gene25443:CDS 35.0%
!!! GTTGTTTTCTAGTTCTTGTC+AGG - chr8.2:40861192-40861211 MS.gene25443:CDS 35.0%
!!! TGCTTTTTCCGTCATGTTTT+TGG - chr8.2:40861447-40861466 MS.gene25443:CDS 35.0%
AAATTCCTAACATGTCACCC+GGG - chr8.2:40862268-40862287 MS.gene25443:CDS 40.0%
AACAGAAGCAGCATCATGTA+GGG - chr8.2:40861563-40861582 MS.gene25443:CDS 40.0%
AATCTCCTGTTAACCTTCCT+CGG + chr8.2:40861313-40861332 None:intergenic 40.0%
AATTCATGGGTTCCTAGTGA+AGG - chr8.2:40862555-40862574 MS.gene25443:CDS 40.0%
AGAGAGATTGCAATTCTAGC+TGG - chr8.2:40862027-40862046 MS.gene25443:CDS 40.0%
AGAGCTTCCGCAACATAATT+CGG - chr8.2:40861695-40861714 MS.gene25443:CDS 40.0%
ATGCTTCATGTGTTCGGATA+AGG - chr8.2:40861858-40861877 MS.gene25443:CDS 40.0%
CAACGAATGCTTCATGTGTT+CGG - chr8.2:40861852-40861871 MS.gene25443:CDS 40.0%
CATGAAATTCATGCCGGATT+TGG - chr8.2:40861240-40861259 MS.gene25443:CDS 40.0%
CTAGAATTGCAATCTCTCTG+AGG + chr8.2:40862026-40862045 None:intergenic 40.0%
CTAGCAAGTAGATTCCATTG+TGG + chr8.2:40862585-40862604 None:intergenic 40.0%
GAAAAATCGCAGCATTCGTT+CGG - chr8.2:40861472-40861491 MS.gene25443:CDS 40.0%
GAAATTCCTAACATGTCACC+CGG - chr8.2:40862267-40862286 MS.gene25443:CDS 40.0%
GCACTTTGGCAATCATTCAA+TGG + chr8.2:40861415-40861434 None:intergenic 40.0%
GTACCGAACTTCTTGTGTTT+GGG - chr8.2:40861896-40861915 MS.gene25443:CDS 40.0%
GTGGATGCATTTACCTAGAA+TGG - chr8.2:40861825-40861844 MS.gene25443:CDS 40.0%
GTTTATAATTGCGCCGTGAT+GGG - chr8.2:40862624-40862643 MS.gene25443:CDS 40.0%
TGTTTATAATTGCGCCGTGA+TGG - chr8.2:40862623-40862642 MS.gene25443:CDS 40.0%
TTCGGATAAGGAGTCTTTAG+CGG - chr8.2:40861870-40861889 MS.gene25443:CDS 40.0%
TTTACAACAGCAACTAGAGG+TGG + chr8.2:40862336-40862355 None:intergenic 40.0%
! ACAGTCTTTTGACAAACTCG+TGG - chr8.2:40861950-40861969 MS.gene25443:CDS 40.0%
! AGAAGAAGCAGCTTGATGAT+GGG - chr8.2:40861506-40861525 MS.gene25443:CDS 40.0%
! CAACAACATTTTCCGCACTT+TGG + chr8.2:40861429-40861448 None:intergenic 40.0%
! CAGTCTTTTGACAAACTCGT+GGG - chr8.2:40861951-40861970 MS.gene25443:CDS 40.0%
! TTATTGTTATTGGCGGACCA+AGG - chr8.2:40862505-40862524 MS.gene25443:CDS 40.0%
!! TCATCATCCAGATCGATTTG+CGG + chr8.2:40861373-40861392 None:intergenic 40.0%
ACAGAAGCAGCATCATGTAG+GGG - chr8.2:40861564-40861583 MS.gene25443:CDS 45.0%
AGATGGGCATATCCTTGACT+CGG - chr8.2:40862062-40862081 MS.gene25443:CDS 45.0%
CAGAAGAAGCAGCTTGATGA+TGG - chr8.2:40861505-40861524 MS.gene25443:CDS 45.0%
CATAATCGGACCTCGAACAA+CGG + chr8.2:40861654-40861673 None:intergenic 45.0%
CATTCGTTGGAAGCCATTCT+AGG + chr8.2:40861841-40861860 None:intergenic 45.0%
CCTATGATCCAATTCGCGAA+CGG - chr8.2:40861803-40861822 MS.gene25443:CDS 45.0%
CCTCCCAAACACAAGAAGTT+CGG + chr8.2:40861902-40861921 None:intergenic 45.0%
CGAACTTCTTGTGTTTGGGA+GGG - chr8.2:40861900-40861919 MS.gene25443:CDS 45.0%
CGAGGCAAGTGAACCATAAT+CGG + chr8.2:40861668-40861687 None:intergenic 45.0%
CTCCATGTCAGCATAATCAG+CGG + chr8.2:40862373-40862392 None:intergenic 45.0%
CTGGTGGTTGTGATTCAGAT+GGG - chr8.2:40862046-40862065 MS.gene25443:CDS 45.0%
GAACAGAAGCAGCATCATGT+AGG - chr8.2:40861562-40861581 MS.gene25443:CDS 45.0%
GAGGAAGGTTAACAGGAGAT+TGG - chr8.2:40861312-40861331 MS.gene25443:CDS 45.0%
GATAAGGAGTCTTTAGCGGT+AGG - chr8.2:40861874-40861893 MS.gene25443:CDS 45.0%
GATTCCAAGCTTCTTACGTG+CGG - chr8.2:40862210-40862229 MS.gene25443:CDS 45.0%
GCAAGTAGATTCCATTGTGG+AGG + chr8.2:40862582-40862601 None:intergenic 45.0%
GCAGAACCAAACAAGCATCT+TGG + chr8.2:40862000-40862019 None:intergenic 45.0%
TATCGTTGGCGAAGACTGAA+CGG - chr8.2:40861730-40861749 MS.gene25443:CDS 45.0%
TATGTTATCGGGGGAATTGG+TGG - chr8.2:40862183-40862202 MS.gene25443:CDS 45.0%
TCCTGGGCTTGATCATGTTT+GGG + chr8.2:40862128-40862147 None:intergenic 45.0%
TCGATCTGGATGATGATCAG+AGG - chr8.2:40861377-40861396 MS.gene25443:CDS 45.0%
TCTGGATGATGATCAGAGGA+AGG - chr8.2:40861381-40861400 MS.gene25443:CDS 45.0%
TGAACGGTATCATAGAGCAC+TGG - chr8.2:40861746-40861765 MS.gene25443:CDS 45.0%
TGTTGTTCCGCAAATCGATC+TGG - chr8.2:40861363-40861382 MS.gene25443:CDS 45.0%
TTCCGCAACATAATTCGGAG+CGG - chr8.2:40861700-40861719 MS.gene25443:CDS 45.0%
TTCCTGGGCTTGATCATGTT+TGG + chr8.2:40862129-40862148 None:intergenic 45.0%
TTGACAAACTCGTGGGAATC+TGG - chr8.2:40861958-40861977 MS.gene25443:CDS 45.0%
! CATTTAGGGCATGTGGAGAT+AGG - chr8.2:40862481-40862500 MS.gene25443:CDS 45.0%
! CGAACACATGAAGCATTCGT+TGG + chr8.2:40861854-40861873 None:intergenic 45.0%
! GAGATTGCAATTCTAGCTGG+TGG - chr8.2:40862030-40862049 MS.gene25443:CDS 45.0%
! GGTACCGAACTTCTTGTGTT+TGG - chr8.2:40861895-40861914 MS.gene25443:CDS 45.0%
!! TTCTAGTTCTTGTCAGGCAG+AGG - chr8.2:40861198-40861217 MS.gene25443:CDS 45.0%
AAACAACCTCGGAACCAAGC+AGG + chr8.2:40861179-40861198 None:intergenic 50.0%
ACGGTTGGGGTCTTGCATTT+AGG - chr8.2:40862466-40862485 MS.gene25443:CDS 50.0%
AGGCAGAGGCAGAGAACAAT+TGG - chr8.2:40861212-40861231 MS.gene25443:CDS 50.0%
ATAGGCCTCCCATTCAAGGA+GGG + chr8.2:40861788-40861807 None:intergenic 50.0%
ATGATCCAATTCGCGAACGG+TGG - chr8.2:40861806-40861825 MS.gene25443:CDS 50.0%
CACCGCTCCGAATTATGTTG+CGG + chr8.2:40861705-40861724 None:intergenic 50.0%
CATTGTGGAGGTCCTTCACT+AGG + chr8.2:40862570-40862589 None:intergenic 50.0%
CCGAACTTCTTGTGTTTGGG+AGG - chr8.2:40861899-40861918 MS.gene25443:CDS 50.0%
CCGTTCGCGAATTGGATCAT+AGG + chr8.2:40861806-40861825 None:intergenic 50.0%
CGGTTGGGGTCTTGCATTTA+GGG - chr8.2:40862467-40862486 MS.gene25443:CDS 50.0%
CGTTGTTCGAGGTCCGATTA+TGG - chr8.2:40861652-40861671 MS.gene25443:CDS 50.0%
CTAGTGAAGGACCTCCACAA+TGG - chr8.2:40862568-40862587 MS.gene25443:CDS 50.0%
GATCATAGGCCTCCCATTCA+AGG + chr8.2:40861792-40861811 None:intergenic 50.0%
GCTGGTGGTTGTGATTCAGA+TGG - chr8.2:40862045-40862064 MS.gene25443:CDS 50.0%
TAATTCCACTGACGAGCCGA+CGG - chr8.2:40861537-40861556 MS.gene25443:CDS 50.0%
TATAGAGCTCAGCCGAGTCA+AGG + chr8.2:40862077-40862096 None:intergenic 50.0%
TCCCAAACATGATCAAGCCC+AGG - chr8.2:40862124-40862143 MS.gene25443:CDS 50.0%
TGGTAGATTACCGGAGAGAG+CGG - chr8.2:40862434-40862453 MS.gene25443:CDS 50.0%
TTCCTGTGCCCTCCTTGAAT+GGG - chr8.2:40861777-40861796 MS.gene25443:CDS 50.0%
TTTCCTGTGCCCTCCTTGAA+TGG - chr8.2:40861776-40861795 MS.gene25443:CDS 50.0%
! ACTCGTGGGAATCTGGAATG+AGG - chr8.2:40861965-40861984 MS.gene25443:CDS 50.0%
! ACTTTTGCCGCGCATGAATC+GGG - chr8.2:40861606-40861625 MS.gene25443:CDS 50.0%
! CACTTTTGCCGCGCATGAAT+CGG - chr8.2:40861605-40861624 MS.gene25443:CDS 50.0%
! CGCTGATTATGCTGACATGG+AGG - chr8.2:40862371-40862390 MS.gene25443:CDS 50.0%
! GGTCTTGCATTTAGGGCATG+TGG - chr8.2:40862474-40862493 MS.gene25443:CDS 50.0%
! TGCTGCTTCTGTTCATCCGT+CGG + chr8.2:40861556-40861575 None:intergenic 50.0%
!! GATGTGCTCTGGGGTATTCA+TGG - chr8.2:40862149-40862168 MS.gene25443:CDS 50.0%
!! TGCTCTGGGGTATTCATGGA+CGG - chr8.2:40862153-40862172 MS.gene25443:CDS 50.0%
AAACCCTCGCCATGACTCCT+TGG + chr8.2:40862525-40862544 None:intergenic 55.0%
AAGCCCAGGAAGATGTGCTC+TGG - chr8.2:40862138-40862157 MS.gene25443:CDS 55.0%
ACCCCAGAGCACATCTTCCT+GGG + chr8.2:40862144-40862163 None:intergenic 55.0%
AGCAACTAGAGGTGGTGCCT+CGG + chr8.2:40862328-40862347 None:intergenic 55.0%
AGCCCAGGAAGATGTGCTCT+GGG - chr8.2:40862139-40862158 MS.gene25443:CDS 55.0%
ATGAAGATGAACCGCAGCCG+AGG - chr8.2:40861293-40861312 MS.gene25443:CDS 55.0%
CATAGGCCTCCCATTCAAGG+AGG + chr8.2:40861789-40861808 None:intergenic 55.0%
CCTCGAACAACGGCTTAGAC+AGG + chr8.2:40861644-40861663 None:intergenic 55.0%
CCTGTCTAAGCCGTTGTTCG+AGG - chr8.2:40861641-40861660 MS.gene25443:CDS 55.0%
CTGTTAACCTTCCTCGGCTG+CGG + chr8.2:40861307-40861326 None:intergenic 55.0%
GTTGGAAGGTCGTTCCTGCT+TGG - chr8.2:40861162-40861181 MS.gene25443:CDS 55.0%
TACCCCAGAGCACATCTTCC+TGG + chr8.2:40862145-40862164 None:intergenic 55.0%
TGCATCCACCGTTCGCGAAT+TGG + chr8.2:40861814-40861833 None:intergenic 55.0%
! ATTGGCGGACCAAGGAGTCA+TGG - chr8.2:40862513-40862532 MS.gene25443:CDS 55.0%
! CGCCGCTGATTATGCTGACA+TGG - chr8.2:40862368-40862387 MS.gene25443:CDS 55.0%
AGATGAACCGCAGCCGAGGA+AGG - chr8.2:40861297-40861316 MS.gene25443:CDS 60.0%
CAAGCTTCTTACGTGCGGCG+AGG - chr8.2:40862215-40862234 MS.gene25443:CDS 60.0%
CGCAGCCGAGGAAGGTTAAC+AGG - chr8.2:40861305-40861324 MS.gene25443:CDS 60.0%
CTACGCCCGGGTGACATGTT+AGG + chr8.2:40862276-40862295 None:intergenic 60.0%
CTCGCCGCACGTAAGAAGCT+TGG + chr8.2:40862217-40862236 None:intergenic 60.0%
CTGTGCCCTCCTTGAATGGG+AGG - chr8.2:40861780-40861799 MS.gene25443:CDS 60.0%
GAGCTGTCCCGATTCATGCG+CGG + chr8.2:40861616-40861635 None:intergenic 60.0%
GCCCAGGAAGATGTGCTCTG+GGG - chr8.2:40862140-40862159 MS.gene25443:CDS 60.0%
GGACCAAGGAGTCATGGCGA+GGG - chr8.2:40862519-40862538 MS.gene25443:CDS 60.0%
GGAGCGGTGAGCTCTATCGT+TGG - chr8.2:40861716-40861735 MS.gene25443:CDS 60.0%
GTCGTTCCTGCTTGGTTCCG+AGG - chr8.2:40861170-40861189 MS.gene25443:CDS 60.0%
TTCATCGACGCCGCTCTCTC+CGG + chr8.2:40862447-40862466 None:intergenic 60.0%
! CTCCCATTCAAGGAGGGCAC+AGG + chr8.2:40861782-40861801 None:intergenic 60.0%
! GCGGAAGCTCTGATTCAGCG+AGG + chr8.2:40861686-40861705 None:intergenic 60.0%
!! AGCGGCGTCGATGAACGGTT+GGG - chr8.2:40862452-40862471 MS.gene25443:CDS 60.0%
!! GAGAGAGCGGCGTCGATGAA+CGG - chr8.2:40862447-40862466 MS.gene25443:CDS 60.0%
!! TTCATCCGTCGGCTCGTCAG+TGG + chr8.2:40861545-40861564 None:intergenic 60.0%
CGATCCCCTAGCACTACGCC+CGG + chr8.2:40862289-40862308 None:intergenic 65.0%
CGGACCAAGGAGTCATGGCG+AGG - chr8.2:40862518-40862537 MS.gene25443:CDS 65.0%
CGGGCGTAGTGCTAGGGGAT+CGG - chr8.2:40862287-40862306 MS.gene25443:CDS 65.0%
GATCCCCTAGCACTACGCCC+GGG + chr8.2:40862288-40862307 None:intergenic 65.0%
GTCACCCGGGCGTAGTGCTA+GGG - chr8.2:40862281-40862300 MS.gene25443:CDS 65.0%
TAGAGGTGGTGCCTCGGCTG+TGG + chr8.2:40862322-40862341 None:intergenic 65.0%
TCACCCGGGCGTAGTGCTAG+GGG - chr8.2:40862282-40862301 MS.gene25443:CDS 65.0%
TGTCACCCGGGCGTAGTGCT+AGG - chr8.2:40862280-40862299 MS.gene25443:CDS 65.0%
!! GAGCGGCGTCGATGAACGGT+TGG - chr8.2:40862451-40862470 MS.gene25443:CDS 65.0%
!! GCGGCGTCGATGAACGGTTG+GGG - chr8.2:40862453-40862472 MS.gene25443:CDS 65.0%
GGAGATGCCTGCCACAGCCG+AGG - chr8.2:40862308-40862327 MS.gene25443:CDS 70.0%
GGTGGTGCCTCGGCTGTGGC+AGG + chr8.2:40862318-40862337 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 40861160 40862653 40861160 ID=MS.gene25443
chr8.2 mRNA 40861160 40862653 40861160 ID=MS.gene25443.t1;Parent=MS.gene25443
chr8.2 exon 40861160 40862653 40861160 ID=MS.gene25443.t1.exon1;Parent=MS.gene25443.t1
chr8.2 CDS 40861160 40862653 40861160 ID=cds.MS.gene25443.t1;Parent=MS.gene25443.t1
Gene Sequence

>MS.gene25443

ATGTTGGAAGGTCGTTCCTGCTTGGTTCCGAGGTTGTTTTCTAGTTCTTGTCAGGCAGAGGCAGAGAACAATTGGTCTTTCATGAAATTCATGCCGGATTTGGAAATAAAAAACGGAAAGCGAGGTATTGATGATGAAGATGAACCGCAGCCGAGGAAGGTTAACAGGAGATTGGAATCTTGTTATCAAGGTGAGATGGATTCTGTTGTTCCGCAAATCGATCTGGATGATGATCAGAGGAAGGATTTTGAGCCATTGAATGATTGCCAAAGTGCGGAAAATGTTGTTGCTTTTTCCGTCATGTTTTTGGCTGAAAAATCGCAGCATTCGTTCGGTGATGAACTGCAGAAGAAGCAGCTTGATGATGGGAATTTGATTAATTCCACTGACGAGCCGACGGATGAACAGAAGCAGCATCATGTAGGGGATTCATCAGATTCAAGTTCACTTTTGCCGCGCATGAATCGGGACAGCTCGATTACCTGTCTAAGCCGTTGTTCGAGGTCCGATTATGGTTCACTTGCCTCGCTGAATCAGAGCTTCCGCAACATAATTCGGAGCGGTGAGCTCTATCGTTGGCGAAGACTGAACGGTATCATAGAGCACTGGATTTATTTTTCCTGTGCCCTCCTTGAATGGGAGGCCTATGATCCAATTCGCGAACGGTGGATGCATTTACCTAGAATGGCTTCCAACGAATGCTTCATGTGTTCGGATAAGGAGTCTTTAGCGGTAGGTACCGAACTTCTTGTGTTTGGGAGGGAGTTGCAATCCCATGTTATATACAGATACAGTCTTTTGACAAACTCGTGGGAATCTGGAATGAGGATGAATTTTCCAAGATGCTTGTTTGGTTCTGCTAGCCTCAGAGAGATTGCAATTCTAGCTGGTGGTTGTGATTCAGATGGGCATATCCTTGACTCGGCTGAGCTCTATAACTCCGAGAATCAAACATGGGAAACACTCCCAAACATGATCAAGCCCAGGAAGATGTGCTCTGGGGTATTCATGGACGGAAATTTTTATGTTATCGGGGGAATTGGTGGACGCGATTCCAAGCTTCTTACGTGCGGCGAGGAATACAATCTACAAACTAGAACATGGAAAGAAATTCCTAACATGTCACCCGGGCGTAGTGCTAGGGGATCGGAGATGCCTGCCACAGCCGAGGCACCACCTCTAGTTGCTGTTGTAAATAATGAACTATACGCCGCTGATTATGCTGACATGGAGGTTAAAAAGTACGATAAAGGTAACAAAGTATGGCTTACAATTGGTAGATTACCGGAGAGAGCGGCGTCGATGAACGGTTGGGGTCTTGCATTTAGGGCATGTGGAGATAGGCTTATTGTTATTGGCGGACCAAGGAGTCATGGCGAGGGTTTTATTGAACTCAATTCATGGGTTCCTAGTGAAGGACCTCCACAATGGAATCTACTTGCTAGAAAACAATCCGGTAACTTTGTTTATAATTGCGCCGTGATGGGATGCTGA
Protein sequence

>MS.gene25443.t1

MLEGRSCLVPRLFSSSCQAEAENNWSFMKFMPDLEIKNGKRGIDDEDEPQPRKVNRRLESCYQGEMDSVVPQIDLDDDQRKDFEPLNDCQSAENVVAFSVMFLAEKSQHSFGDELQKKQLDDGNLINSTDEPTDEQKQHHVGDSSDSSSLLPRMNRDSSITCLSRCSRSDYGSLASLNQSFRNIIRSGELYRWRRLNGIIEHWIYFSCALLEWEAYDPIRERWMHLPRMASNECFMCSDKESLAVGTELLVFGRELQSHVIYRYSLLTNSWESGMRMNFPRCLFGSASLREIAILAGGCDSDGHILDSAELYNSENQTWETLPNMIKPRKMCSGVFMDGNFYVIGGIGGRDSKLLTCGEEYNLQTRTWKEIPNMSPGRSARGSEMPATAEAPPLVAVVNNELYAADYADMEVKKYDKGNKVWLTIGRLPERAASMNGWGLAFRACGDRLIVIGGPRSHGEGFIELNSWVPSEGPPQWNLLARKQSGNFVYNCAVMGC