Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83931.t1 | XP_003613674.2 | 98.1 | 476 | 9 | 0 | 1 | 476 | 1 | 476 | 8.00E-262 | 912.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83931.t1 | Q8L736 | 59.9 | 486 | 166 | 10 | 1 | 476 | 1 | 467 | 4.3e-139 | 496.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83931.t1 | G7KCH5 | 98.1 | 476 | 9 | 0 | 1 | 476 | 1 | 476 | 5.8e-262 | 912.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055886 | MS.gene83931 | 0.808488 | 3.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene25443 | MS.gene83931 | PPI |
| MS.gene34711 | MS.gene83931 | PPI |
| MS.gene032830 | MS.gene83931 | PPI |
| MS.gene074027 | MS.gene83931 | PPI |
| MS.gene83931 | MS.gene63251 | PPI |
| MS.gene83931 | MS.gene049391 | PPI |
| MS.gene055886 | MS.gene83931 | PPI |
| MS.gene013419 | MS.gene83931 | PPI |
| MS.gene91909 | MS.gene83931 | PPI |
| MS.gene06516 | MS.gene83931 | PPI |
| MS.gene059347 | MS.gene83931 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83931.t1 | MTR_5g039390 | 98.319 | 476 | 8 | 0 | 1 | 476 | 1 | 476 | 0.0 | 972 |
| MS.gene83931.t1 | MTR_8g468030 | 73.870 | 509 | 88 | 7 | 1 | 476 | 1 | 497 | 0.0 | 734 |
| MS.gene83931.t1 | MTR_4g108280 | 63.760 | 367 | 128 | 2 | 112 | 476 | 17 | 380 | 5.42e-173 | 491 |
| MS.gene83931.t1 | MTR_4g022260 | 60.656 | 366 | 143 | 1 | 111 | 476 | 53 | 417 | 6.10e-161 | 462 |
| MS.gene83931.t1 | MTR_0345s0010 | 61.190 | 353 | 136 | 1 | 124 | 476 | 84 | 435 | 7.17e-161 | 462 |
| MS.gene83931.t1 | MTR_0105s0130 | 61.190 | 353 | 136 | 1 | 124 | 476 | 84 | 435 | 2.02e-160 | 461 |
| MS.gene83931.t1 | MTR_0105s0170 | 60.907 | 353 | 137 | 1 | 124 | 476 | 97 | 448 | 9.58e-160 | 460 |
| MS.gene83931.t1 | MTR_0105s0020 | 60.340 | 353 | 139 | 1 | 124 | 476 | 84 | 435 | 3.15e-159 | 458 |
| MS.gene83931.t1 | MTR_0105s0040 | 60.907 | 353 | 137 | 1 | 124 | 476 | 182 | 533 | 4.02e-159 | 461 |
| MS.gene83931.t1 | MTR_0496s0010 | 59.563 | 366 | 141 | 3 | 111 | 476 | 53 | 411 | 6.89e-158 | 454 |
| MS.gene83931.t1 | MTR_1330s0010 | 60.807 | 347 | 135 | 1 | 124 | 470 | 97 | 442 | 6.18e-156 | 450 |
| MS.gene83931.t1 | MTR_4g022400 | 59.322 | 354 | 143 | 1 | 123 | 476 | 62 | 414 | 1.04e-151 | 438 |
| MS.gene83931.t1 | MTR_2g094093 | 50.282 | 354 | 163 | 4 | 128 | 476 | 19 | 364 | 2.87e-115 | 343 |
| MS.gene83931.t1 | MTR_7g029290 | 38.596 | 342 | 193 | 9 | 142 | 474 | 68 | 401 | 2.06e-66 | 219 |
| MS.gene83931.t1 | MTR_6g023805 | 37.534 | 365 | 190 | 9 | 130 | 474 | 57 | 403 | 2.40e-63 | 211 |
| MS.gene83931.t1 | MTR_0105s0060 | 56.044 | 91 | 40 | 0 | 386 | 476 | 15 | 105 | 6.31e-35 | 126 |
| MS.gene83931.t1 | MTR_3g085570 | 28.980 | 245 | 149 | 7 | 125 | 352 | 31 | 267 | 9.75e-22 | 97.1 |
| MS.gene83931.t1 | MTR_8g080370 | 26.446 | 242 | 159 | 5 | 125 | 352 | 47 | 283 | 2.16e-16 | 81.3 |
| MS.gene83931.t1 | MTR_3g073640 | 23.851 | 348 | 191 | 11 | 129 | 408 | 43 | 384 | 1.34e-15 | 79.3 |
| MS.gene83931.t1 | MTR_8g085600 | 26.408 | 284 | 181 | 9 | 129 | 401 | 77 | 343 | 2.76e-15 | 78.2 |
| MS.gene83931.t1 | MTR_4g114110 | 25.243 | 309 | 201 | 11 | 105 | 401 | 57 | 347 | 5.28e-15 | 77.4 |
| MS.gene83931.t1 | MTR_2g008440 | 23.810 | 357 | 191 | 12 | 125 | 408 | 39 | 387 | 1.47e-13 | 73.2 |
| MS.gene83931.t1 | MTR_4g011900 | 24.800 | 250 | 162 | 5 | 129 | 352 | 5 | 254 | 2.00e-13 | 72.0 |
| MS.gene83931.t1 | MTR_7g095220 | 22.511 | 231 | 161 | 4 | 138 | 351 | 13 | 242 | 5.72e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83931.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene83931.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene83931.t1 | AT2G02870 | 60.700 | 486 | 162 | 9 | 1 | 476 | 1 | 467 | 0.0 | 568 |
| MS.gene83931.t1 | AT1G14330 | 59.290 | 479 | 154 | 8 | 1 | 476 | 1 | 441 | 0.0 | 550 |
| MS.gene83931.t1 | AT1G26930 | 68.378 | 370 | 114 | 1 | 110 | 476 | 52 | 421 | 0.0 | 531 |
| MS.gene83931.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene83931.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene83931.t1 | AT1G74510 | 65.169 | 356 | 115 | 2 | 130 | 476 | 96 | 451 | 3.05e-169 | 484 |
| MS.gene83931.t1 | AT1G26930 | 57.838 | 370 | 101 | 2 | 110 | 476 | 52 | 369 | 4.88e-149 | 430 |
| MS.gene83931.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene83931.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene83931.t1 | AT5G60570 | 45.430 | 372 | 188 | 6 | 111 | 476 | 31 | 393 | 4.14e-107 | 324 |
| MS.gene83931.t1 | AT3G27150 | 37.673 | 361 | 198 | 10 | 130 | 474 | 71 | 420 | 9.55e-67 | 220 |
| MS.gene83931.t1 | AT3G27150 | 37.673 | 361 | 198 | 10 | 130 | 474 | 71 | 420 | 9.55e-67 | 220 |
| MS.gene83931.t1 | AT5G40680 | 35.119 | 336 | 206 | 4 | 107 | 437 | 43 | 371 | 4.92e-61 | 205 |
| MS.gene83931.t1 | AT1G16250 | 26.061 | 330 | 208 | 10 | 128 | 433 | 7 | 324 | 2.97e-24 | 104 |
| MS.gene83931.t1 | AT1G16250 | 26.061 | 330 | 208 | 10 | 128 | 433 | 7 | 324 | 2.97e-24 | 104 |
| MS.gene83931.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene83931.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene83931.t1 | AT1G67480 | 29.614 | 233 | 145 | 5 | 129 | 347 | 39 | 266 | 2.45e-21 | 95.9 |
| MS.gene83931.t1 | AT1G15670 | 25.648 | 347 | 209 | 14 | 129 | 445 | 3 | 330 | 7.49e-17 | 82.4 |
| MS.gene83931.t1 | AT1G55270 | 26.496 | 234 | 157 | 5 | 129 | 352 | 78 | 306 | 1.71e-16 | 82.0 |
| MS.gene83931.t1 | AT3G61350 | 23.054 | 334 | 215 | 12 | 115 | 432 | 6 | 313 | 2.20e-14 | 74.7 |
| MS.gene83931.t1 | AT3G61350 | 23.054 | 334 | 215 | 12 | 115 | 432 | 6 | 313 | 2.20e-14 | 74.7 |
| MS.gene83931.t1 | AT1G80440 | 24.540 | 326 | 208 | 11 | 129 | 432 | 3 | 312 | 7.52e-14 | 73.2 |
| MS.gene83931.t1 | AT1G22040 | 25.862 | 348 | 183 | 11 | 129 | 408 | 43 | 383 | 7.56e-14 | 73.9 |
| MS.gene83931.t1 | AT1G27420 | 24.206 | 252 | 150 | 7 | 124 | 350 | 5 | 240 | 4.60e-12 | 67.8 |
| MS.gene83931.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 4 | 214 | 2.97e-11 | 65.1 |
| MS.gene83931.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 11 | 221 | 3.01e-11 | 65.1 |
| MS.gene83931.t1 | AT3G63220 | 23.113 | 212 | 145 | 4 | 129 | 323 | 11 | 221 | 3.01e-11 | 65.1 |
Find 120 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGGAGATTCGTCGGATTC+TGG | 0.248232 | 5.4:+29240806 | MS.gene83931:CDS |
| TGCATCCACCTTTGGCGAAT+TGG | 0.251940 | 5.4:-29241037 | None:intergenic |
| ATGGTTGGGGTCTTGCATTT+AGG | 0.276947 | 5.4:+29241692 | MS.gene83931:CDS |
| TGGTTGGGGTCTTGCATTTA+GGG | 0.285142 | 5.4:+29241693 | MS.gene83931:CDS |
| GGAAATATGCTCATTGTTAT+TGG | 0.306561 | 5.4:+29241721 | MS.gene83931:CDS |
| CATGAAGTACATGCTGGATT+TGG | 0.313702 | 5.4:+29240523 | MS.gene83931:CDS |
| TTCATCGACATCGCTCGTTC+TGG | 0.316008 | 5.4:-29241670 | None:intergenic |
| GGAACCGAACTTCTTGTGTT+TGG | 0.324378 | 5.4:+29241121 | MS.gene83931:CDS |
| GTTTCATTGAACTCAATTCT+TGG | 0.330175 | 5.4:+29241767 | MS.gene83931:CDS |
| AGGAATTTCTGTCCATGTTC+TGG | 0.338733 | 5.4:-29241479 | None:intergenic |
| ATCTATAAACCTCGCCGCTT+CGG | 0.350189 | 5.4:-29240940 | None:intergenic |
| ATGCTGACATGGAAGTTAAA+AGG | 0.357590 | 5.4:+29241605 | MS.gene83931:CDS |
| GAACCGAACTTCTTGTGTTT+GGG | 0.358419 | 5.4:+29241122 | MS.gene83931:CDS |
| GCATCCACCTTTGGCGAATT+GGG | 0.360483 | 5.4:-29241036 | None:intergenic |
| CGCTGTTCAAGGTCTGATTA+TGG | 0.366547 | 5.4:+29240878 | MS.gene83931:CDS |
| TGTGTCTCAGTCGCTGTTCA+AGG | 0.376165 | 5.4:+29240867 | MS.gene83931:CDS |
| TCTCGTGTGCCCTCCTTGAA+TGG | 0.387707 | 5.4:+29241002 | MS.gene83931:CDS |
| TTTCATTGAACTCAATTCTT+GGG | 0.403492 | 5.4:+29241768 | MS.gene83931:CDS |
| TTGACAAATTCATGGTCCTC+TGG | 0.419803 | 5.4:+29241184 | MS.gene83931:CDS |
| TCGGCAAATGCATCCACCTT+TGG | 0.425014 | 5.4:-29241045 | None:intergenic |
| CGTTTATAATTGTGCTGTGA+TGG | 0.426012 | 5.4:+29241849 | MS.gene83931:CDS |
| ACAAACATTCAAGGGCTCAT+TGG | 0.427663 | 5.4:-29240708 | None:intergenic |
| CTCGTGTGCCCTCCTTGAAT+GGG | 0.432187 | 5.4:+29241003 | MS.gene83931:CDS |
| TTCCTCGGTTTATTCATGCT+CGG | 0.436739 | 5.4:-29241352 | None:intergenic |
| GGCGGTGCCTCGGTTGCAGC+AGG | 0.445232 | 5.4:-29241541 | None:intergenic |
| AAACCGAGGAAGATGTGTTC+TGG | 0.445291 | 5.4:+29241364 | MS.gene83931:CDS |
| ATTAGCAGGTGGTTGTGATT+CGG | 0.446104 | 5.4:+29241267 | MS.gene83931:CDS |
| TCCTCGGTTTATTCATGCTC+GGG | 0.446460 | 5.4:-29241351 | None:intergenic |
| GGATTCTGTTCTTTCTCAAA+CGG | 0.450478 | 5.4:+29240676 | MS.gene83931:CDS |
| AGCGATGTCGATGAATGGTT+GGG | 0.451823 | 5.4:+29241678 | MS.gene83931:CDS |
| AAACGGTATCATGGAACATT+GGG | 0.454394 | 5.4:+29240973 | MS.gene83931:CDS |
| ACAATTATAAACGAAGTTAC+CGG | 0.457419 | 5.4:-29241839 | None:intergenic |
| GGTCGTACGCTTCCCATTCA+AGG | 0.463650 | 5.4:-29241015 | None:intergenic |
| GATGTGTTCTGGTGTATTCA+TGG | 0.466838 | 5.4:+29241375 | MS.gene83931:CDS |
| AGAACCCCTACTACTTCGTC+CGG | 0.468311 | 5.4:-29241512 | None:intergenic |
| TGAGAAAGAACAGAATCCTT+AGG | 0.471913 | 5.4:-29240671 | None:intergenic |
| AACTCCTTGACAAACATTCA+AGG | 0.473412 | 5.4:-29240717 | None:intergenic |
| AATGCTCCAAGATGTTTGTT+CGG | 0.474359 | 5.4:+29241217 | MS.gene83931:CDS |
| CGCCGCTTCGGATTATTTCA+CGG | 0.477509 | 5.4:-29240928 | None:intergenic |
| GAGCGATGTCGATGAATGGT+TGG | 0.478296 | 5.4:+29241677 | MS.gene83931:CDS |
| ATCTTGTCACATAGTTGAAA+CGG | 0.478670 | 5.4:+29240628 | MS.gene83931:CDS |
| TATTTCGTTGCAACATCCTA+AGG | 0.480105 | 5.4:+29240655 | MS.gene83931:CDS |
| GTCGTTGCTGTGTTGTTTCG+AGG | 0.487767 | 5.4:+29240459 | MS.gene83931:CDS |
| TGAGATGCCTGCTGCAACCG+AGG | 0.492241 | 5.4:+29241534 | MS.gene83931:CDS |
| GAAGCGGCGAGGTTTATAGA+TGG | 0.493954 | 5.4:+29240942 | MS.gene83931:CDS |
| GGTGAGATTGCGATATTAGC+AGG | 0.494746 | 5.4:+29241253 | MS.gene83931:CDS |
| AAACCTTCACCATGAGTCCT+AGG | 0.500082 | 5.4:-29241748 | None:intergenic |
| GATAAAGAATCTTTAGCTGT+AGG | 0.501866 | 5.4:+29241100 | MS.gene83931:CDS |
| GTCTTACATGAAGTACATGC+TGG | 0.509303 | 5.4:+29240517 | MS.gene83931:CDS |
| GGCAATGAGAGGCGGTGCCT+CGG | 0.509621 | 5.4:-29241551 | None:intergenic |
| AACATCTTAGTACACTTCCT+AGG | 0.510935 | 5.4:-29240602 | None:intergenic |
| AATATGCTCATTGTTATTGG+TGG | 0.514731 | 5.4:+29241724 | MS.gene83931:CDS |
| CCGAACTTCTTGTGTTTGGG+AGG | 0.516315 | 5.4:+29241125 | MS.gene83931:CDS |
| TCTGATGAGGAGCAGCATGC+TGG | 0.518838 | 5.4:+29240788 | MS.gene83931:CDS |
| CCTCCCAAACACAAGAAGTT+CGG | 0.521950 | 5.4:-29241125 | None:intergenic |
| GTGGATGCATTTGCCGAGGA+TGG | 0.521975 | 5.4:+29241051 | MS.gene83931:CDS |
| GCAGAGCCGAACAAACATCT+TGG | 0.522841 | 5.4:-29241223 | None:intergenic |
| CTGAGGATGAACATCATCCT+AGG | 0.526740 | 5.4:+29240585 | MS.gene83931:CDS |
| TATAGATGGAGGAGACTAAA+CGG | 0.529185 | 5.4:+29240956 | MS.gene83931:CDS |
| GTTTATAATTGTGCTGTGAT+GGG | 0.533513 | 5.4:+29241850 | MS.gene83931:CDS |
| TAAACGGTATCATGGAACAT+TGG | 0.533894 | 5.4:+29240972 | MS.gene83931:CDS |
| GAGGAGACTAAACGGTATCA+TGG | 0.537620 | 5.4:+29240964 | MS.gene83931:CDS |
| GAACGAGCGATGTCGATGAA+TGG | 0.544102 | 5.4:+29241673 | MS.gene83931:CDS |
| CGAACTTCTTGTGTTTGGGA+GGG | 0.544855 | 5.4:+29241126 | MS.gene83931:CDS |
| TAGGAAGTGTACTAAGATGT+TGG | 0.544939 | 5.4:+29240604 | MS.gene83931:CDS |
| GAACCCCTACTACTTCGTCC+GGG | 0.545858 | 5.4:-29241511 | None:intergenic |
| ACACCAGAACACATCTTCCT+CGG | 0.549483 | 5.4:-29241367 | None:intergenic |
| GACACACGATTGAGCTGTCT+CGG | 0.549997 | 5.4:-29240850 | None:intergenic |
| TTGTTCGGCTCTGCAAGCCT+TGG | 0.557116 | 5.4:+29241232 | MS.gene83931:CDS |
| CATTGCGGAGGTCCTTCACT+AGG | 0.564491 | 5.4:-29241793 | None:intergenic |
| CTACTAGCTCGAAAACGATC+CGG | 0.564520 | 5.4:+29241820 | MS.gene83931:CDS |
| GAAATTCCTGACATGTCCCC+CGG | 0.564869 | 5.4:+29241493 | MS.gene83931:CDS |
| CTTGTGAAACAGAGAACGAT+TGG | 0.567058 | 5.4:+29240495 | MS.gene83931:CDS |
| GAGCTGTCTCGGTTCATCCT+CGG | 0.567515 | 5.4:-29240839 | None:intergenic |
| AGCAGGTGGTTGTGATTCGG+AGG | 0.569771 | 5.4:+29241270 | MS.gene83931:CDS |
| CAATCGTTTGAAGCCATCCT+CGG | 0.574471 | 5.4:-29241064 | None:intergenic |
| TGTTCTGGTGTATTCATGGA+TGG | 0.587848 | 5.4:+29241379 | MS.gene83931:CDS |
| AGAAAACTGTGGATTACTGT+TGG | 0.588100 | 5.4:+29241640 | MS.gene83931:CDS |
| TGAAATAATCCGAAGCGGCG+AGG | 0.588835 | 5.4:+29240931 | MS.gene83931:CDS |
| ACGACCCAATTCGCCAAAGG+TGG | 0.595170 | 5.4:+29241032 | MS.gene83931:CDS |
| AATTCTTGGGTGCCTAGTGA+AGG | 0.596232 | 5.4:+29241781 | MS.gene83931:CDS |
| GCAGCATGCTGGAGATTCGT+CGG | 0.597982 | 5.4:+29240799 | MS.gene83931:CDS |
| TGCTGCTGATTATGCTGACA+TGG | 0.602364 | 5.4:+29241594 | MS.gene83931:CDS |
| GCGATGTCGATGAATGGTTG+GGG | 0.603196 | 5.4:+29241679 | MS.gene83931:CDS |
| AGCATTCATTCTCATTCCAG+AGG | 0.606098 | 5.4:-29241200 | None:intergenic |
| TGTTATTCAGCAATCTGATG+AGG | 0.609072 | 5.4:+29240775 | MS.gene83931:CDS |
| GTTTGTCAAGGAGTTCATGA+TGG | 0.609659 | 5.4:+29240725 | MS.gene83931:CDS |
| ACTCCTTGACAAACATTCAA+GGG | 0.609745 | 5.4:-29240716 | None:intergenic |
| TCAGCAGAATCAATATTCAT+CGG | 0.615157 | 5.4:-29240566 | None:intergenic |
| GAGCCCTTGAATGTTTGTCA+AGG | 0.615186 | 5.4:+29240713 | MS.gene83931:CDS |
| GGTCTTGCATTTAGGGCATG+CGG | 0.616506 | 5.4:+29241700 | MS.gene83931:CDS |
| ATTGGTGGACCTAGGACTCA+TGG | 0.617047 | 5.4:+29241739 | MS.gene83931:CDS |
| AACCCCTACTACTTCGTCCG+GGG | 0.618769 | 5.4:-29241510 | None:intergenic |
| ATAATTTACAAACCAGAACA+TGG | 0.619596 | 5.4:+29241467 | MS.gene83931:CDS |
| CTCAGAATTGTATAGTTCAG+CGG | 0.626222 | 5.4:-29241308 | None:intergenic |
| CTAGTGAAGGACCTCCGCAA+TGG | 0.629280 | 5.4:+29241794 | MS.gene83931:CDS |
| ACCCCTACTACTTCGTCCGG+GGG | 0.630121 | 5.4:-29241509 | None:intergenic |
| CGAGCTAGTAGACGCCATTG+CGG | 0.631973 | 5.4:-29241808 | None:intergenic |
| TTATCTACAACGGCAATGAG+AGG | 0.633005 | 5.4:-29241562 | None:intergenic |
| GCAGGTGGTTGTGATTCGGA+GGG | 0.633783 | 5.4:+29241271 | MS.gene83931:CDS |
| TCATTGTTATTGGTGGACCT+AGG | 0.638584 | 5.4:+29241731 | MS.gene83931:CDS |
| GGACCTAGGACTCATGGTGA+AGG | 0.644982 | 5.4:+29241745 | MS.gene83931:CDS |
| TTCCGTGAAATAATCCGAAG+CGG | 0.645763 | 5.4:+29240926 | MS.gene83931:CDS |
| GCGGCGAGGTTTATAGATGG+AGG | 0.647194 | 5.4:+29240945 | MS.gene83931:CDS |
| CGTACGACCCAATTCGCCAA+AGG | 0.656928 | 5.4:+29241029 | MS.gene83931:CDS |
| AAAGGTGGATGCATTTGCCG+AGG | 0.658304 | 5.4:+29241047 | MS.gene83931:CDS |
| GCTAGTAGACGCCATTGCGG+AGG | 0.660404 | 5.4:-29241805 | None:intergenic |
| ATACAATTCATTATCTACAA+CGG | 0.661324 | 5.4:-29241572 | None:intergenic |
| TGTCAAGGAGTTCATGATGG+TGG | 0.663431 | 5.4:+29240728 | MS.gene83931:CDS |
| TATGTTGTAGGTGGAATCGG+CGG | 0.671001 | 5.4:+29241409 | MS.gene83931:CDS |
| CGTACGCTTCCCATTCAAGG+AGG | 0.678294 | 5.4:-29241012 | None:intergenic |
| GTACGCTTCCCATTCAAGGA+GGG | 0.679998 | 5.4:-29241011 | None:intergenic |
| TCTACAACGGCAATGAGAGG+CGG | 0.681780 | 5.4:-29241559 | None:intergenic |
| GGAAGTTAAAAGGTATGACA+AGG | 0.683430 | 5.4:+29241615 | MS.gene83931:CDS |
| GATGAATATTGATTCTGCTG+AGG | 0.684071 | 5.4:+29240568 | MS.gene83931:CDS |
| CTAATATCGCAATCTCACCA+AGG | 0.689793 | 5.4:-29241249 | None:intergenic |
| ATGACAAGGAAAGAAAACTG+TGG | 0.706266 | 5.4:+29241629 | MS.gene83931:CDS |
| TCCCGAGCATGAATAAACCG+AGG | 0.713762 | 5.4:+29241350 | MS.gene83931:CDS |
| GATTCAAAGCTTCTTACATG+CGG | 0.719497 | 5.4:+29241436 | MS.gene83931:CDS |
| GAGATTGCGATATTAGCAGG+TGG | 0.734472 | 5.4:+29241256 | MS.gene83931:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACAATTCATTATCTACAA+CGG | - | chr5.4:29241575-29241594 | None:intergenic | 20.0% |
| ! | ACAATTATAAACGAAGTTAC+CGG | - | chr5.4:29241842-29241861 | None:intergenic | 25.0% |
| ! | ATAATTTACAAACCAGAACA+TGG | + | chr5.4:29241467-29241486 | MS.gene83931:CDS | 25.0% |
| ! | TTTCATTGAACTCAATTCTT+GGG | + | chr5.4:29241768-29241787 | MS.gene83931:CDS | 25.0% |
| !! | GATGGAAAATTTTATGTTGT+AGG | + | chr5.4:29241397-29241416 | MS.gene83931:CDS | 25.0% |
| ATCTTGTCACATAGTTGAAA+CGG | + | chr5.4:29240628-29240647 | MS.gene83931:CDS | 30.0% | |
| GATAAAGAATCTTTAGCTGT+AGG | + | chr5.4:29241100-29241119 | MS.gene83931:CDS | 30.0% | |
| GGAAATATGCTCATTGTTAT+TGG | + | chr5.4:29241721-29241740 | MS.gene83931:CDS | 30.0% | |
| GTTTATAATTGTGCTGTGAT+GGG | + | chr5.4:29241850-29241869 | MS.gene83931:CDS | 30.0% | |
| GTTTCATTGAACTCAATTCT+TGG | + | chr5.4:29241767-29241786 | MS.gene83931:CDS | 30.0% | |
| TCAGCAGAATCAATATTCAT+CGG | - | chr5.4:29240569-29240588 | None:intergenic | 30.0% | |
| ! | AATATGCTCATTGTTATTGG+TGG | + | chr5.4:29241724-29241743 | MS.gene83931:CDS | 30.0% |
| ! | ACAGTCTTTTGACAAATTCA+TGG | + | chr5.4:29241176-29241195 | MS.gene83931:CDS | 30.0% |
| ! | GGAAAATTTTATGTTGTAGG+TGG | + | chr5.4:29241400-29241419 | MS.gene83931:CDS | 30.0% |
| ! | TTTTATGTTGTAGGTGGAAT+CGG | + | chr5.4:29241406-29241425 | MS.gene83931:CDS | 30.0% |
| AAACGGTATCATGGAACATT+GGG | + | chr5.4:29240973-29240992 | MS.gene83931:CDS | 35.0% | |
| AACATCTTAGTACACTTCCT+AGG | - | chr5.4:29240605-29240624 | None:intergenic | 35.0% | |
| AACTCCTTGACAAACATTCA+AGG | - | chr5.4:29240720-29240739 | None:intergenic | 35.0% | |
| AATGCTCCAAGATGTTTGTT+CGG | + | chr5.4:29241217-29241236 | MS.gene83931:CDS | 35.0% | |
| ACTCCTTGACAAACATTCAA+GGG | - | chr5.4:29240719-29240738 | None:intergenic | 35.0% | |
| ATGACAAGGAAAGAAAACTG+TGG | + | chr5.4:29241629-29241648 | MS.gene83931:CDS | 35.0% | |
| ATGCTGACATGGAAGTTAAA+AGG | + | chr5.4:29241605-29241624 | MS.gene83931:CDS | 35.0% | |
| CGTTTATAATTGTGCTGTGA+TGG | + | chr5.4:29241849-29241868 | MS.gene83931:CDS | 35.0% | |
| CTCAGAATTGTATAGTTCAG+CGG | - | chr5.4:29241311-29241330 | None:intergenic | 35.0% | |
| CTGGATTTGGACATCAAAAA+TGG | + | chr5.4:29240536-29240555 | MS.gene83931:CDS | 35.0% | |
| GATTCAAAGCTTCTTACATG+CGG | + | chr5.4:29241436-29241455 | MS.gene83931:CDS | 35.0% | |
| GGATTCTGTTCTTTCTCAAA+CGG | + | chr5.4:29240676-29240695 | MS.gene83931:CDS | 35.0% | |
| TAAACGGTATCATGGAACAT+TGG | + | chr5.4:29240972-29240991 | MS.gene83931:CDS | 35.0% | |
| TATAGATGGAGGAGACTAAA+CGG | + | chr5.4:29240956-29240975 | MS.gene83931:CDS | 35.0% | |
| TATTTCGTTGCAACATCCTA+AGG | + | chr5.4:29240655-29240674 | MS.gene83931:CDS | 35.0% | |
| TGAGAAAGAACAGAATCCTT+AGG | - | chr5.4:29240674-29240693 | None:intergenic | 35.0% | |
| TGTTATTCAGCAATCTGATG+AGG | + | chr5.4:29240775-29240794 | MS.gene83931:CDS | 35.0% | |
| ! | AGAAAACTGTGGATTACTGT+TGG | + | chr5.4:29241640-29241659 | MS.gene83931:CDS | 35.0% |
| ! | GGAAGTTAAAAGGTATGACA+AGG | + | chr5.4:29241615-29241634 | MS.gene83931:CDS | 35.0% |
| ! | TAGGAAGTGTACTAAGATGT+TGG | + | chr5.4:29240604-29240623 | MS.gene83931:CDS | 35.0% |
| !! | GATGAATATTGATTCTGCTG+AGG | + | chr5.4:29240568-29240587 | MS.gene83931:CDS | 35.0% |
| ACAAACATTCAAGGGCTCAT+TGG | - | chr5.4:29240711-29240730 | None:intergenic | 40.0% | |
| AGCATTCATTCTCATTCCAG+AGG | - | chr5.4:29241203-29241222 | None:intergenic | 40.0% | |
| AGGAATTTCTGTCCATGTTC+TGG | - | chr5.4:29241482-29241501 | None:intergenic | 40.0% | |
| ATTAGCAGGTGGTTGTGATT+CGG | + | chr5.4:29241267-29241286 | MS.gene83931:CDS | 40.0% | |
| CATGAAGTACATGCTGGATT+TGG | + | chr5.4:29240523-29240542 | MS.gene83931:CDS | 40.0% | |
| CTAATATCGCAATCTCACCA+AGG | - | chr5.4:29241252-29241271 | None:intergenic | 40.0% | |
| CTTGTGAAACAGAGAACGAT+TGG | + | chr5.4:29240495-29240514 | MS.gene83931:CDS | 40.0% | |
| GAACCGAACTTCTTGTGTTT+GGG | + | chr5.4:29241122-29241141 | MS.gene83931:CDS | 40.0% | |
| GATGTGTTCTGGTGTATTCA+TGG | + | chr5.4:29241375-29241394 | MS.gene83931:CDS | 40.0% | |
| GTCTTACATGAAGTACATGC+TGG | + | chr5.4:29240517-29240536 | MS.gene83931:CDS | 40.0% | |
| GTTTGTCAAGGAGTTCATGA+TGG | + | chr5.4:29240725-29240744 | MS.gene83931:CDS | 40.0% | |
| TGTTCTGGTGTATTCATGGA+TGG | + | chr5.4:29241379-29241398 | MS.gene83931:CDS | 40.0% | |
| TTATCTACAACGGCAATGAG+AGG | - | chr5.4:29241565-29241584 | None:intergenic | 40.0% | |
| TTCCGTGAAATAATCCGAAG+CGG | + | chr5.4:29240926-29240945 | MS.gene83931:CDS | 40.0% | |
| TTCCTCGGTTTATTCATGCT+CGG | - | chr5.4:29241355-29241374 | None:intergenic | 40.0% | |
| TTGACAAATTCATGGTCCTC+TGG | + | chr5.4:29241184-29241203 | MS.gene83931:CDS | 40.0% | |
| ! | TCATTGTTATTGGTGGACCT+AGG | + | chr5.4:29241731-29241750 | MS.gene83931:CDS | 40.0% |
| !!! | ATTCTGGTTCTCTTTTACCG+AGG | + | chr5.4:29240822-29240841 | MS.gene83931:CDS | 40.0% |
| AAACCGAGGAAGATGTGTTC+TGG | + | chr5.4:29241364-29241383 | MS.gene83931:CDS | 45.0% | |
| AAACCTTCACCATGAGTCCT+AGG | - | chr5.4:29241751-29241770 | None:intergenic | 45.0% | |
| AATTCTTGGGTGCCTAGTGA+AGG | + | chr5.4:29241781-29241800 | MS.gene83931:CDS | 45.0% | |
| ACACCAGAACACATCTTCCT+CGG | - | chr5.4:29241370-29241389 | None:intergenic | 45.0% | |
| AGCGATGTCGATGAATGGTT+GGG | + | chr5.4:29241678-29241697 | MS.gene83931:CDS | 45.0% | |
| ATCTATAAACCTCGCCGCTT+CGG | - | chr5.4:29240943-29240962 | None:intergenic | 45.0% | |
| ATGGTTGGGGTCTTGCATTT+AGG | + | chr5.4:29241692-29241711 | MS.gene83931:CDS | 45.0% | |
| CCTCCCAAACACAAGAAGTT+CGG | - | chr5.4:29241128-29241147 | None:intergenic | 45.0% | |
| CGAACTTCTTGTGTTTGGGA+GGG | + | chr5.4:29241126-29241145 | MS.gene83931:CDS | 45.0% | |
| CGCTGTTCAAGGTCTGATTA+TGG | + | chr5.4:29240878-29240897 | MS.gene83931:CDS | 45.0% | |
| CTACTAGCTCGAAAACGATC+CGG | + | chr5.4:29241820-29241839 | MS.gene83931:CDS | 45.0% | |
| CTGAGGATGAACATCATCCT+AGG | + | chr5.4:29240585-29240604 | MS.gene83931:CDS | 45.0% | |
| GAGATTGCGATATTAGCAGG+TGG | + | chr5.4:29241256-29241275 | MS.gene83931:CDS | 45.0% | |
| GAGCCCTTGAATGTTTGTCA+AGG | + | chr5.4:29240713-29240732 | MS.gene83931:CDS | 45.0% | |
| GAGGAGACTAAACGGTATCA+TGG | + | chr5.4:29240964-29240983 | MS.gene83931:CDS | 45.0% | |
| GGAACCGAACTTCTTGTGTT+TGG | + | chr5.4:29241121-29241140 | MS.gene83931:CDS | 45.0% | |
| GGTGAGATTGCGATATTAGC+AGG | + | chr5.4:29241253-29241272 | MS.gene83931:CDS | 45.0% | |
| TATGTTGTAGGTGGAATCGG+CGG | + | chr5.4:29241409-29241428 | MS.gene83931:CDS | 45.0% | |
| TCCTCGGTTTATTCATGCTC+GGG | - | chr5.4:29241354-29241373 | None:intergenic | 45.0% | |
| TGGTTGGGGTCTTGCATTTA+GGG | + | chr5.4:29241693-29241712 | MS.gene83931:CDS | 45.0% | |
| TGTCAAGGAGTTCATGATGG+TGG | + | chr5.4:29240728-29240747 | MS.gene83931:CDS | 45.0% | |
| ! | CAATCGTTTGAAGCCATCCT+CGG | - | chr5.4:29241067-29241086 | None:intergenic | 45.0% |
| ! | TGCTGCTGATTATGCTGACA+TGG | + | chr5.4:29241594-29241613 | MS.gene83931:CDS | 45.0% |
| AAAGGTGGATGCATTTGCCG+AGG | + | chr5.4:29241047-29241066 | MS.gene83931:CDS | 50.0% | |
| AGAACCCCTACTACTTCGTC+CGG | - | chr5.4:29241515-29241534 | None:intergenic | 50.0% | |
| CCGAACTTCTTGTGTTTGGG+AGG | + | chr5.4:29241125-29241144 | MS.gene83931:CDS | 50.0% | |
| CGCCGCTTCGGATTATTTCA+CGG | - | chr5.4:29240931-29240950 | None:intergenic | 50.0% | |
| GAAATTCCTGACATGTCCCC+CGG | + | chr5.4:29241493-29241512 | MS.gene83931:CDS | 50.0% | |
| GAACGAGCGATGTCGATGAA+TGG | + | chr5.4:29241673-29241692 | MS.gene83931:CDS | 50.0% | |
| GAAGCGGCGAGGTTTATAGA+TGG | + | chr5.4:29240942-29240961 | MS.gene83931:CDS | 50.0% | |
| GACACACGATTGAGCTGTCT+CGG | - | chr5.4:29240853-29240872 | None:intergenic | 50.0% | |
| GAGCGATGTCGATGAATGGT+TGG | + | chr5.4:29241677-29241696 | MS.gene83931:CDS | 50.0% | |
| GCAGAGCCGAACAAACATCT+TGG | - | chr5.4:29241226-29241245 | None:intergenic | 50.0% | |
| GCATCCACCTTTGGCGAATT+GGG | - | chr5.4:29241039-29241058 | None:intergenic | 50.0% | |
| GCGATGTCGATGAATGGTTG+GGG | + | chr5.4:29241679-29241698 | MS.gene83931:CDS | 50.0% | |
| GTACGCTTCCCATTCAAGGA+GGG | - | chr5.4:29241014-29241033 | None:intergenic | 50.0% | |
| TCCCGAGCATGAATAAACCG+AGG | + | chr5.4:29241350-29241369 | MS.gene83931:CDS | 50.0% | |
| TCGGCAAATGCATCCACCTT+TGG | - | chr5.4:29241048-29241067 | None:intergenic | 50.0% | |
| TCTACAACGGCAATGAGAGG+CGG | - | chr5.4:29241562-29241581 | None:intergenic | 50.0% | |
| TGAAATAATCCGAAGCGGCG+AGG | + | chr5.4:29240931-29240950 | MS.gene83931:CDS | 50.0% | |
| TGCATCCACCTTTGGCGAAT+TGG | - | chr5.4:29241040-29241059 | None:intergenic | 50.0% | |
| TGTGTCTCAGTCGCTGTTCA+AGG | + | chr5.4:29240867-29240886 | MS.gene83931:CDS | 50.0% | |
| TTCATCGACATCGCTCGTTC+TGG | - | chr5.4:29241673-29241692 | None:intergenic | 50.0% | |
| ! | ATTGGTGGACCTAGGACTCA+TGG | + | chr5.4:29241739-29241758 | MS.gene83931:CDS | 50.0% |
| ! | GGTCTTGCATTTAGGGCATG+CGG | + | chr5.4:29241700-29241719 | MS.gene83931:CDS | 50.0% |
| !! | GTCGTTGCTGTGTTGTTTCG+AGG | + | chr5.4:29240459-29240478 | MS.gene83931:CDS | 50.0% |
| AACCCCTACTACTTCGTCCG+GGG | - | chr5.4:29241513-29241532 | None:intergenic | 55.0% | |
| ACGACCCAATTCGCCAAAGG+TGG | + | chr5.4:29241032-29241051 | MS.gene83931:CDS | 55.0% | |
| CATTGCGGAGGTCCTTCACT+AGG | - | chr5.4:29241796-29241815 | None:intergenic | 55.0% | |
| CGAGCTAGTAGACGCCATTG+CGG | - | chr5.4:29241811-29241830 | None:intergenic | 55.0% | |
| CGTACGACCCAATTCGCCAA+AGG | + | chr5.4:29241029-29241048 | MS.gene83931:CDS | 55.0% | |
| CGTACGCTTCCCATTCAAGG+AGG | - | chr5.4:29241015-29241034 | None:intergenic | 55.0% | |
| CTAGTGAAGGACCTCCGCAA+TGG | + | chr5.4:29241794-29241813 | MS.gene83931:CDS | 55.0% | |
| CTCGTGTGCCCTCCTTGAAT+GGG | + | chr5.4:29241003-29241022 | MS.gene83931:CDS | 55.0% | |
| GAACCCCTACTACTTCGTCC+GGG | - | chr5.4:29241514-29241533 | None:intergenic | 55.0% | |
| GAGCTGTCTCGGTTCATCCT+CGG | - | chr5.4:29240842-29240861 | None:intergenic | 55.0% | |
| GCAGCATGCTGGAGATTCGT+CGG | + | chr5.4:29240799-29240818 | MS.gene83931:CDS | 55.0% | |
| GCGGCGAGGTTTATAGATGG+AGG | + | chr5.4:29240945-29240964 | MS.gene83931:CDS | 55.0% | |
| GCTGGAGATTCGTCGGATTC+TGG | + | chr5.4:29240806-29240825 | MS.gene83931:CDS | 55.0% | |
| GGACCTAGGACTCATGGTGA+AGG | + | chr5.4:29241745-29241764 | MS.gene83931:CDS | 55.0% | |
| GGTCGTACGCTTCCCATTCA+AGG | - | chr5.4:29241018-29241037 | None:intergenic | 55.0% | |
| GTGGATGCATTTGCCGAGGA+TGG | + | chr5.4:29241051-29241070 | MS.gene83931:CDS | 55.0% | |
| TCTCGTGTGCCCTCCTTGAA+TGG | + | chr5.4:29241002-29241021 | MS.gene83931:CDS | 55.0% | |
| TCTGATGAGGAGCAGCATGC+TGG | + | chr5.4:29240788-29240807 | MS.gene83931:CDS | 55.0% | |
| TTGTTCGGCTCTGCAAGCCT+TGG | + | chr5.4:29241232-29241251 | MS.gene83931:CDS | 55.0% | |
| ! | AGCAGGTGGTTGTGATTCGG+AGG | + | chr5.4:29241270-29241289 | MS.gene83931:CDS | 55.0% |
| ! | GCAGGTGGTTGTGATTCGGA+GGG | + | chr5.4:29241271-29241290 | MS.gene83931:CDS | 55.0% |
| ACCCCTACTACTTCGTCCGG+GGG | - | chr5.4:29241512-29241531 | None:intergenic | 60.0% | |
| GCTAGTAGACGCCATTGCGG+AGG | - | chr5.4:29241808-29241827 | None:intergenic | 60.0% | |
| GTCCCCCGGACGAAGTAGTA+GGG | + | chr5.4:29241507-29241526 | MS.gene83931:CDS | 60.0% | |
| TCCCCCGGACGAAGTAGTAG+GGG | + | chr5.4:29241508-29241527 | MS.gene83931:CDS | 60.0% | |
| TGAGATGCCTGCTGCAACCG+AGG | + | chr5.4:29241534-29241553 | MS.gene83931:CDS | 60.0% | |
| TGTCCCCCGGACGAAGTAGT+AGG | + | chr5.4:29241506-29241525 | MS.gene83931:CDS | 60.0% | |
| CTTCGTCCGGGGGACATGTC+AGG | - | chr5.4:29241502-29241521 | None:intergenic | 65.0% | |
| GGCAATGAGAGGCGGTGCCT+CGG | - | chr5.4:29241554-29241573 | None:intergenic | 65.0% | |
| GGCGGTGCCTCGGTTGCAGC+AGG | - | chr5.4:29241544-29241563 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 29240449 | 29241879 | 29240449 | ID=MS.gene83931 |
| chr5.4 | mRNA | 29240449 | 29241879 | 29240449 | ID=MS.gene83931.t1;Parent=MS.gene83931 |
| chr5.4 | exon | 29240449 | 29241879 | 29240449 | ID=MS.gene83931.t1.exon1;Parent=MS.gene83931.t1 |
| chr5.4 | CDS | 29240449 | 29241879 | 29240449 | ID=cds.MS.gene83931.t1;Parent=MS.gene83931.t1 |
| Gene Sequence |
| Protein sequence |
