Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07506.t1 | XP_013468867.1 | 99.5 | 419 | 2 | 0 | 1 | 419 | 102 | 520 | 1.80E-201 | 711.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07506.t1 | Q9FHY8 | 69.4 | 421 | 125 | 3 | 1 | 419 | 147 | 565 | 2.3e-128 | 460.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07506.t1 | A0A072VLP9 | 99.5 | 419 | 2 | 0 | 1 | 419 | 102 | 520 | 1.3e-201 | 711.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049411 | MS.gene07506 | 0.808455 | 3.07E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene07506 | 0.818648 | 1.76E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene07506 | 0.882086 | 1.40E-70 | -1.69E-46 |
| MS.gene051003 | MS.gene07506 | 0.850417 | 1.72E-60 | -1.69E-46 |
| MS.gene051234 | MS.gene07506 | 0.828269 | 9.87E-55 | -1.69E-46 |
| MS.gene051381 | MS.gene07506 | 0.804102 | 2.54E-49 | -1.69E-46 |
| MS.gene051642 | MS.gene07506 | 0.814176 | 1.76E-51 | -1.69E-46 |
| MS.gene051850 | MS.gene07506 | 0.809882 | 1.52E-50 | -1.69E-46 |
| MS.gene052296 | MS.gene07506 | 0.803529 | 3.34E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene07506 | 0.812789 | 3.55E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene07506 | 0.810498 | 1.12E-50 | -1.69E-46 |
| MS.gene053952 | MS.gene07506 | 0.809692 | 1.67E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene07506 | 0.813109 | 3.02E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene07506 | 0.803374 | 3.59E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene07506 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene057094 | MS.gene07506 | 0.821102 | 4.83E-53 | -1.69E-46 |
| MS.gene057234 | MS.gene07506 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene058654 | MS.gene07506 | 0.808541 | 2.94E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene07506 | 0.803457 | 3.46E-49 | -1.69E-46 |
| MS.gene059227 | MS.gene07506 | 0.835988 | 1.22E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene07506 | 0.804433 | 2.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07506.t1 | MTR_1g077850 | 99.284 | 419 | 3 | 0 | 1 | 419 | 102 | 520 | 0.0 | 862 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07506.t1 | AT5G41950 | 75.414 | 423 | 96 | 4 | 1 | 419 | 147 | 565 | 0.0 | 656 |
Find 105 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTCAGGCGCTTAATAAT+TGG | 0.142305 | 1.3:-55576856 | MS.gene07506:intron |
| GAGCTAACATCTTCTCTTTC+GGG | 0.200894 | 1.3:+55580970 | None:intergenic |
| CGGTTAAATATCCAACTTTA+AGG | 0.206145 | 1.3:+55573900 | None:intergenic |
| TGGCTTCTTACTGAATAATT+TGG | 0.228506 | 1.3:+55574578 | None:intergenic |
| CACCGGGCAATTTACAATCT+TGG | 0.286151 | 1.3:-55574849 | MS.gene07506:CDS |
| TACAGTGCATCATAATCTTC+AGG | 0.287071 | 1.3:+55579831 | None:intergenic |
| AAGATGTTAGCTCTCCTTAC+AGG | 0.291498 | 1.3:-55580960 | MS.gene07506:intron |
| ATTTCAGGCGCTTAATAATT+GGG | 0.322345 | 1.3:-55576855 | MS.gene07506:intron |
| AGAGCTAACATCTTCTCTTT+CGG | 0.335566 | 1.3:+55580969 | None:intergenic |
| TAATTGGGGACTTGCGCTTC+AGG | 0.337641 | 1.3:-55576840 | MS.gene07506:intron |
| TGCTGACTCTTGGGAATCAT+TGG | 0.349651 | 1.3:-55572830 | MS.gene07506:CDS |
| TTACAATCTTGGAACTGTTC+TGG | 0.353539 | 1.3:-55574838 | MS.gene07506:intron |
| ATTATGATGCACTGTACAAT+TGG | 0.360131 | 1.3:-55579824 | MS.gene07506:CDS |
| CTGACTTTGCCACCTGGTGC+TGG | 0.360992 | 1.3:-55573532 | MS.gene07506:CDS |
| GATTGGCTGTACAATTCATT+TGG | 0.366257 | 1.3:+55574635 | None:intergenic |
| CAATTGGGCATTGGTACTTC+AGG | 0.369935 | 1.3:-55579808 | MS.gene07506:intron |
| GCTGTTGCTGTTGACTATCT+TGG | 0.373402 | 1.3:+55580156 | None:intergenic |
| TTAGAAGGTGAAGTGGAATC+TGG | 0.383082 | 1.3:+55578294 | None:intergenic |
| ACCATGAGAGAATTGCTTCA+TGG | 0.392969 | 1.3:-55581012 | MS.gene07506:CDS |
| GCAGAGGACACTTTAAGAAC+AGG | 0.403120 | 1.3:-55574686 | MS.gene07506:CDS |
| TGCTTGCCTCTGTTCAACTC+TGG | 0.404309 | 1.3:+55573657 | None:intergenic |
| TTATCAAGGTTGCTGACTCT+TGG | 0.405651 | 1.3:-55572840 | MS.gene07506:intron |
| CTTGCGGTTGGTACGATCTA+TGG | 0.407275 | 1.3:-55574360 | MS.gene07506:intron |
| TTAAAGCAGTCCAACTCAAC+TGG | 0.408233 | 1.3:-55577092 | MS.gene07506:CDS |
| GTATCCAGGCTTACTACAAT+TGG | 0.408555 | 1.3:-55577428 | MS.gene07506:intron |
| ATTCATGGCATCTCCTGATC+TGG | 0.410632 | 1.3:+55573618 | None:intergenic |
| TCAAGCAGAGCATCTTTAGA+AGG | 0.411824 | 1.3:+55578279 | None:intergenic |
| CAAAGTCTCTCAACCAGATC+AGG | 0.413385 | 1.3:-55573631 | MS.gene07506:CDS |
| TATCAAGGTTGCTGACTCTT+GGG | 0.414988 | 1.3:-55572839 | MS.gene07506:intron |
| CTCTGCATTGATACAGTTCA+TGG | 0.422436 | 1.3:-55573508 | MS.gene07506:CDS |
| AGCTGAAGAGCTATGGAAAC+AGG | 0.425760 | 1.3:-55577358 | MS.gene07506:intron |
| GTATTGGCGGGTATTATAAC+AGG | 0.435968 | 1.3:-55572748 | MS.gene07506:CDS |
| CCGCTACCACACCTTAAAGT+TGG | 0.441526 | 1.3:-55573911 | MS.gene07506:CDS |
| GATCAGGAGATGCCATGAAT+GGG | 0.443681 | 1.3:-55573615 | MS.gene07506:CDS |
| GGAATCATTGGATGGATGGC+TGG | 0.453718 | 1.3:-55572818 | MS.gene07506:CDS |
| AGATCAGGAGATGCCATGAA+TGG | 0.456962 | 1.3:-55573616 | MS.gene07506:CDS |
| TATTTAACCGCACCTCCTGC+GGG | 0.458286 | 1.3:-55573887 | MS.gene07506:CDS |
| GATCGTTTCCAGTCATTATG+AGG | 0.464366 | 1.3:+55573851 | None:intergenic |
| AGTTCATGGATCAGTTTACT+TGG | 0.466595 | 1.3:-55573494 | MS.gene07506:intron |
| GAGTTGATAAACAGCGTTGT+AGG | 0.474591 | 1.3:-55580107 | MS.gene07506:CDS |
| ACTCAACTGGAACAGTCCTC+AGG | 0.484214 | 1.3:-55577079 | MS.gene07506:intron |
| AGTTGCAATTTGATTTCCAC+CGG | 0.493039 | 1.3:-55574866 | MS.gene07506:CDS |
| ATATTTAACCGCACCTCCTG+CGG | 0.501162 | 1.3:-55573888 | MS.gene07506:CDS |
| GCTTGCCTCTGTTCAACTCT+GGG | 0.504813 | 1.3:+55573658 | None:intergenic |
| CAAAGGAAGCTGAAGAGCTA+TGG | 0.504826 | 1.3:-55577365 | MS.gene07506:CDS |
| GGATCACGAAAAGCTTCAGC+AGG | 0.507858 | 1.3:-55573816 | MS.gene07506:intron |
| CTTGGGAATCATTGGATGGA+TGG | 0.512023 | 1.3:-55572822 | MS.gene07506:CDS |
| CTGCAATATATATTGCAGAT+TGG | 0.512331 | 1.3:+55574618 | None:intergenic |
| ACAGTGCATCATAATCTTCA+GGG | 0.517083 | 1.3:+55579832 | None:intergenic |
| TTCCAAGATTGTAAATTGCC+CGG | 0.521530 | 1.3:+55574847 | None:intergenic |
| GCTGATCTGACTTTGCCACC+TGG | 0.523003 | 1.3:-55573538 | MS.gene07506:CDS |
| GAGGACTGTTCCAGTTGAGT+TGG | 0.523242 | 1.3:+55577082 | None:intergenic |
| GAAATCAAATTGCAACTGTA+TGG | 0.525917 | 1.3:+55574872 | None:intergenic |
| TATCCAGGCTTACTACAATT+GGG | 0.527161 | 1.3:-55577427 | MS.gene07506:intron |
| AGAACATAACAATGCTGCTA+TGG | 0.535089 | 1.3:-55580129 | MS.gene07506:CDS |
| TTGATGAGGAGGGACGGTCT+AGG | 0.539601 | 1.3:-55580082 | MS.gene07506:CDS |
| TTATGATGCACTGTACAATT+GGG | 0.539658 | 1.3:-55579823 | MS.gene07506:CDS |
| TTTCAGGCGCTTAATAATTG+GGG | 0.541226 | 1.3:-55576854 | MS.gene07506:intron |
| GACTCTTGGGAATCATTGGA+TGG | 0.547060 | 1.3:-55572826 | MS.gene07506:CDS |
| TAGTTGCACCTCATAATGAC+TGG | 0.549693 | 1.3:-55573859 | MS.gene07506:CDS |
| CAGAGGACACTTTAAGAACA+GGG | 0.561148 | 1.3:-55574685 | MS.gene07506:CDS |
| GCGAGGCAAAAGTGATGTAT+TGG | 0.562011 | 1.3:-55572764 | MS.gene07506:CDS |
| GGCAAAAGTGATGTATTGGC+GGG | 0.563534 | 1.3:-55572760 | MS.gene07506:CDS |
| TTCTAAAGATGCTCTGCTTG+AGG | 0.564390 | 1.3:-55578277 | MS.gene07506:CDS |
| ATTGCAACTGTATGGCTGAA+CGG | 0.567248 | 1.3:+55574880 | None:intergenic |
| AGGCAAAAGTGATGTATTGG+CGG | 0.568848 | 1.3:-55572761 | MS.gene07506:CDS |
| GCTCTTCAGCTTCCTTTGTG+CGG | 0.573547 | 1.3:+55577370 | None:intergenic |
| AGCATCTTTAGAAGGTGAAG+TGG | 0.579092 | 1.3:+55578287 | None:intergenic |
| CGTTGTAGGTGTTGATGAGG+AGG | 0.579367 | 1.3:-55580093 | MS.gene07506:CDS |
| GTCTACACTATTTATGTGCG+AGG | 0.583038 | 1.3:-55572781 | MS.gene07506:CDS |
| GGTTGAGCGGAAAACCTGTA+AGG | 0.583881 | 1.3:+55580946 | None:intergenic |
| AGGAGGGACGGTCTAGGCAA+CGG | 0.587494 | 1.3:-55580076 | MS.gene07506:CDS |
| CATCATGTAGTGTGGGACAA+AGG | 0.588356 | 1.3:+55578223 | None:intergenic |
| TCCATGAAGCAATTCTCTCA+TGG | 0.592727 | 1.3:+55581011 | None:intergenic |
| CTTACATACATCATGTAGTG+TGG | 0.595760 | 1.3:+55578215 | None:intergenic |
| AAATATCCAACTTTAAGGTG+TGG | 0.603114 | 1.3:+55573905 | None:intergenic |
| TCAATGCAGAGGCCAGCACC+AGG | 0.604478 | 1.3:+55573520 | None:intergenic |
| CAGCGTTGTAGGTGTTGATG+AGG | 0.612108 | 1.3:-55580096 | MS.gene07506:CDS |
| TGTTTGCATGCAGGAAAGTG+CGG | 0.614003 | 1.3:-55578328 | MS.gene07506:intron |
| TTTAACCGCACCTCCTGCGG+GGG | 0.615781 | 1.3:-55573885 | MS.gene07506:CDS |
| TTCTTACATTTGCTGCCAAG+AGG | 0.617598 | 1.3:-55580052 | MS.gene07506:intron |
| CCAACTTTAAGGTGTGGTAG+CGG | 0.619422 | 1.3:+55573911 | None:intergenic |
| ATATAGCAGTGCCTTGCGGT+TGG | 0.626613 | 1.3:-55574372 | MS.gene07506:CDS |
| TTACATACATCATGTAGTGT+GGG | 0.629806 | 1.3:+55578216 | None:intergenic |
| TAGGTGTTGATGAGGAGGGA+CGG | 0.640085 | 1.3:-55580088 | MS.gene07506:CDS |
| GGTATGCTGCTGCTATTGAG+AGG | 0.640973 | 1.3:-55579857 | MS.gene07506:CDS |
| TGCACTGTACAATTGGGCAT+TGG | 0.643214 | 1.3:-55579817 | MS.gene07506:CDS |
| ATCAGGAGATGCCATGAATG+GGG | 0.644427 | 1.3:-55573614 | MS.gene07506:CDS |
| ATTTAACCGCACCTCCTGCG+GGG | 0.650150 | 1.3:-55573886 | MS.gene07506:CDS |
| GAGGACACTTTAAGAACAGG+GGG | 0.652005 | 1.3:-55574683 | MS.gene07506:CDS |
| CATGAACTGTATCAATGCAG+AGG | 0.654472 | 1.3:+55573509 | None:intergenic |
| TGTACGAACTGCTATCAGCA+AGG | 0.656315 | 1.3:-55575019 | MS.gene07506:intron |
| GTTGTAGGTGTTGATGAGGA+GGG | 0.661388 | 1.3:-55580092 | MS.gene07506:CDS |
| CATAGATCGTACCAACCGCA+AGG | 0.665616 | 1.3:+55574361 | None:intergenic |
| GTTGCAATTTGATTTCCACC+GGG | 0.682764 | 1.3:-55574865 | MS.gene07506:CDS |
| TCTGATCGTGCAAAGATGCG+AGG | 0.685281 | 1.3:-55577393 | MS.gene07506:CDS |
| AAAGATGCGAGGCCGCACAA+AGG | 0.686655 | 1.3:-55577382 | MS.gene07506:CDS |
| TTTACAGTACGGATTAGCAG+AGG | 0.686728 | 1.3:-55574702 | MS.gene07506:intron |
| ATGCAGAGGCCAGCACCAGG+TGG | 0.687236 | 1.3:+55573523 | None:intergenic |
| AGGTATATAGCAGTGCCTTG+CGG | 0.692086 | 1.3:-55574376 | MS.gene07506:CDS |
| TAGTGTGGGACAAAGGCGAG+TGG | 0.693067 | 1.3:+55578230 | None:intergenic |
| CAAGATTGTAAATTGCCCGG+TGG | 0.696463 | 1.3:+55574850 | None:intergenic |
| AGAGGACACTTTAAGAACAG+GGG | 0.707286 | 1.3:-55574684 | MS.gene07506:CDS |
| AGGTACCCAGAGTTGAACAG+AGG | 0.741985 | 1.3:-55573663 | MS.gene07506:CDS |
| AGGACACTTTAAGAACAGGG+GGG | 0.806890 | 1.3:-55574682 | MS.gene07506:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 55572744 | 55581031 | 55572744 | ID=MS.gene07506 |
| chr1.3 | mRNA | 55572744 | 55581031 | 55572744 | ID=MS.gene07506.t1;Parent=MS.gene07506 |
| chr1.3 | exon | 55580961 | 55581031 | 55580961 | ID=MS.gene07506.t1.exon1;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55580961 | 55581031 | 55580961 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55580053 | 55580178 | 55580053 | ID=MS.gene07506.t1.exon2;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55580053 | 55580178 | 55580053 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55579809 | 55579878 | 55579809 | ID=MS.gene07506.t1.exon3;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55579809 | 55579878 | 55579809 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55578224 | 55578337 | 55578224 | ID=MS.gene07506.t1.exon4;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55578224 | 55578337 | 55578224 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55577359 | 55577442 | 55577359 | ID=MS.gene07506.t1.exon5;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55577359 | 55577442 | 55577359 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55577080 | 55577130 | 55577080 | ID=MS.gene07506.t1.exon6;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55577080 | 55577130 | 55577080 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55576841 | 55576870 | 55576841 | ID=MS.gene07506.t1.exon7;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55576841 | 55576870 | 55576841 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55575020 | 55575082 | 55575020 | ID=MS.gene07506.t1.exon8;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55575020 | 55575082 | 55575020 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55574839 | 55574904 | 55574839 | ID=MS.gene07506.t1.exon9;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55574839 | 55574904 | 55574839 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55574589 | 55574717 | 55574589 | ID=MS.gene07506.t1.exon10;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55574589 | 55574717 | 55574589 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55574361 | 55574396 | 55574361 | ID=MS.gene07506.t1.exon11;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55574361 | 55574396 | 55574361 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55573817 | 55573936 | 55573817 | ID=MS.gene07506.t1.exon12;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55573817 | 55573936 | 55573817 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55573495 | 55573683 | 55573495 | ID=MS.gene07506.t1.exon13;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55573495 | 55573683 | 55573495 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| chr1.3 | exon | 55572744 | 55572854 | 55572744 | ID=MS.gene07506.t1.exon14;Parent=MS.gene07506.t1 |
| chr1.3 | CDS | 55572744 | 55572854 | 55572744 | ID=cds.MS.gene07506.t1;Parent=MS.gene07506.t1 |
| Gene Sequence |
| Protein sequence |
