Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051373.t1 | RHN48636.1 | 54.6 | 456 | 8 | 1 | 1 | 257 | 1 | 456 | 5.50E-116 | 427.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051373.t1 | Q9SD53 | 46.8 | 124 | 62 | 3 | 39 | 159 | 34 | 156 | 4.7e-23 | 109.8 |
| MS.gene051373.t1 | Q9SD53 | 32.0 | 125 | 79 | 2 | 133 | 256 | 341 | 460 | 1.5e-13 | 78.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051373.t1 | A0A396H5T2 | 54.6 | 456 | 8 | 1 | 1 | 257 | 1 | 456 | 4.0e-116 | 427.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051370 | MS.gene051373 | 0.855172 | 7.54E-62 | -1.69E-46 |
| MS.gene051373 | MS.gene051983 | 0.807974 | 3.89E-50 | -1.69E-46 |
| MS.gene051373 | MS.gene053494 | 0.818656 | 1.75E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene055367 | 0.83056 | 2.74E-55 | -1.69E-46 |
| MS.gene051373 | MS.gene055701 | 0.830009 | 3.74E-55 | -1.69E-46 |
| MS.gene051373 | MS.gene056979 | 0.802525 | 5.38E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene061343 | 0.835014 | 2.15E-56 | -1.69E-46 |
| MS.gene051373 | MS.gene061506 | 0.802067 | 6.69E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene064293 | 0.831227 | 1.88E-55 | -1.69E-46 |
| MS.gene051373 | MS.gene065350 | -0.819551 | 1.09E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene067274 | -0.80885 | 2.53E-50 | -1.69E-46 |
| MS.gene051373 | MS.gene068128 | 0.815663 | 8.24E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene074393 | -0.834226 | 3.39E-56 | -1.69E-46 |
| MS.gene051373 | MS.gene07548 | 0.801099 | 1.06E-48 | -1.69E-46 |
| MS.gene051373 | MS.gene08779 | 0.834184 | 3.47E-56 | -1.69E-46 |
| MS.gene051373 | MS.gene08906 | 0.831837 | 1.33E-55 | -1.69E-46 |
| MS.gene051373 | MS.gene08908 | 0.825668 | 4.13E-54 | -1.69E-46 |
| MS.gene051373 | MS.gene08932 | 0.818956 | 1.50E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene09310 | 0.828229 | 1.01E-54 | -1.69E-46 |
| MS.gene051373 | MS.gene09558 | 0.840961 | 6.34E-58 | -1.69E-46 |
| MS.gene051373 | MS.gene24337 | 0.800055 | 1.72E-48 | -1.69E-46 |
| MS.gene051373 | MS.gene25201 | 0.816059 | 6.72E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene25913 | -0.835764 | 1.39E-56 | -1.69E-46 |
| MS.gene051373 | MS.gene26487 | 0.829944 | 3.88E-55 | -1.69E-46 |
| MS.gene051373 | MS.gene26514 | 0.807214 | 5.64E-50 | -1.69E-46 |
| MS.gene051373 | MS.gene28532 | 0.803178 | 3.95E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene31116 | 0.80105 | 1.08E-48 | -1.69E-46 |
| MS.gene051373 | MS.gene31671 | 0.802382 | 5.76E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene32732 | 0.812828 | 3.48E-51 | -1.69E-46 |
| MS.gene051373 | MS.gene33198 | 0.803809 | 2.92E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene39430 | 0.803748 | 3.01E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene39638 | 0.808025 | 3.79E-50 | -1.69E-46 |
| MS.gene051373 | MS.gene39685 | 0.828125 | 1.07E-54 | -1.69E-46 |
| MS.gene051373 | MS.gene40031 | 0.818812 | 1.61E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene41037 | 0.807807 | 4.22E-50 | -1.69E-46 |
| MS.gene051373 | MS.gene42668 | 0.826232 | 3.04E-54 | -1.69E-46 |
| MS.gene051373 | MS.gene48030 | 0.808375 | 3.20E-50 | -1.69E-46 |
| MS.gene051373 | MS.gene52059 | 0.804981 | 1.66E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene55041 | 0.812459 | 4.19E-51 | -1.69E-46 |
| MS.gene051373 | MS.gene55357 | 0.80144 | 9.00E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene57183 | 0.832772 | 7.81E-56 | -1.69E-46 |
| MS.gene051373 | MS.gene57950 | 0.820073 | 8.31E-53 | -1.69E-46 |
| MS.gene051373 | MS.gene57951 | 0.81858 | 1.82E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene58503 | 0.814388 | 1.58E-51 | -1.69E-46 |
| MS.gene051373 | MS.gene58652 | 0.841012 | 6.15E-58 | -1.69E-46 |
| MS.gene051373 | MS.gene58789 | 0.84273 | 2.16E-58 | -1.69E-46 |
| MS.gene051373 | MS.gene59149 | 0.804815 | 1.80E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene62587 | 0.801847 | 7.42E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene66208 | 0.849176 | 3.82E-60 | -1.69E-46 |
| MS.gene051373 | MS.gene67316 | 0.801814 | 7.54E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene68525 | 0.803875 | 2.83E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene68528 | 0.817763 | 2.79E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene70586 | -0.839674 | 1.38E-57 | -1.69E-46 |
| MS.gene051373 | MS.gene71595 | -0.810831 | 9.48E-51 | -1.69E-46 |
| MS.gene051373 | MS.gene72054 | 0.864106 | 1.54E-64 | -1.69E-46 |
| MS.gene051373 | MS.gene73759 | 0.825414 | 4.75E-54 | -1.69E-46 |
| MS.gene051373 | MS.gene75626 | 0.817532 | 3.14E-52 | -1.69E-46 |
| MS.gene051373 | MS.gene77579 | 0.800444 | 1.44E-48 | -1.69E-46 |
| MS.gene051373 | MS.gene80157 | 0.821185 | 4.62E-53 | -1.69E-46 |
| MS.gene051373 | MS.gene83082 | 0.823081 | 1.68E-53 | -1.69E-46 |
| MS.gene051373 | MS.gene95070 | 0.801313 | 9.55E-49 | -1.69E-46 |
| MS.gene051373 | MS.gene95377 | 0.825256 | 5.18E-54 | -1.69E-46 |
| MS.gene051373 | MS.gene95457 | 0.811223 | 7.79E-51 | -1.69E-46 |
| MS.gene051373 | MS.gene98959 | 0.822111 | 2.82E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051373.t1 | MTR_5g016570 | 44.275 | 131 | 68 | 3 | 25 | 152 | 76 | 204 | 2.00e-27 | 110 |
| MS.gene051373.t1 | MTR_5g016570 | 42.424 | 99 | 57 | 0 | 157 | 255 | 432 | 530 | 4.47e-16 | 77.8 |
| MS.gene051373.t1 | MTR_3g079390 | 47.115 | 104 | 54 | 1 | 51 | 153 | 40 | 143 | 5.35e-22 | 94.7 |
| MS.gene051373.t1 | MTR_6g009170 | 38.938 | 113 | 69 | 0 | 49 | 161 | 23 | 135 | 2.94e-21 | 92.4 |
| MS.gene051373.t1 | MTR_6g009610 | 37.615 | 109 | 68 | 0 | 43 | 151 | 17 | 125 | 9.18e-21 | 90.9 |
| MS.gene051373.t1 | MTR_5g086230 | 40.385 | 104 | 59 | 2 | 52 | 152 | 29 | 132 | 1.65e-20 | 86.7 |
| MS.gene051373.t1 | MTR_6g010920 | 39.604 | 101 | 61 | 0 | 51 | 151 | 25 | 125 | 2.12e-20 | 89.7 |
| MS.gene051373.t1 | MTR_6g009590 | 37.615 | 109 | 68 | 0 | 43 | 151 | 17 | 125 | 3.07e-20 | 89.4 |
| MS.gene051373.t1 | MTR_6g011060 | 37.864 | 103 | 64 | 0 | 51 | 153 | 25 | 127 | 8.96e-20 | 88.2 |
| MS.gene051373.t1 | MTR_6g011050 | 38.318 | 107 | 66 | 0 | 51 | 157 | 24 | 130 | 9.38e-20 | 87.8 |
| MS.gene051373.t1 | MTR_5g086340 | 41.346 | 104 | 58 | 1 | 52 | 152 | 33 | 136 | 4.41e-19 | 86.3 |
| MS.gene051373.t1 | MTR_6g010890 | 35.075 | 134 | 85 | 1 | 51 | 182 | 25 | 158 | 4.77e-19 | 85.9 |
| MS.gene051373.t1 | MTR_0024s0080 | 39.806 | 103 | 61 | 1 | 52 | 153 | 26 | 128 | 8.91e-18 | 82.4 |
| MS.gene051373.t1 | MTR_7g067940 | 42.718 | 103 | 58 | 1 | 50 | 151 | 28 | 130 | 1.40e-17 | 82.0 |
| MS.gene051373.t1 | MTR_7g068070 | 39.655 | 116 | 65 | 2 | 50 | 160 | 28 | 143 | 4.80e-17 | 80.5 |
| MS.gene051373.t1 | MTR_3g466740 | 35.211 | 142 | 78 | 3 | 30 | 166 | 6 | 138 | 6.33e-17 | 80.1 |
| MS.gene051373.t1 | MTR_3g107590 | 36.364 | 121 | 73 | 2 | 41 | 157 | 14 | 134 | 6.51e-17 | 80.1 |
| MS.gene051373.t1 | MTR_2g036320 | 34.615 | 182 | 100 | 6 | 50 | 223 | 66 | 236 | 2.77e-16 | 78.2 |
| MS.gene051373.t1 | MTR_0067s0010 | 35.338 | 133 | 81 | 2 | 24 | 151 | 13 | 145 | 4.97e-16 | 77.4 |
| MS.gene051373.t1 | MTR_7g067660 | 38.182 | 110 | 60 | 2 | 50 | 151 | 15 | 124 | 1.41e-15 | 75.9 |
| MS.gene051373.t1 | MTR_2g041180 | 37.615 | 109 | 65 | 1 | 50 | 155 | 49 | 157 | 4.94e-15 | 74.7 |
| MS.gene051373.t1 | MTR_6g010810 | 34.314 | 102 | 65 | 1 | 52 | 151 | 23 | 124 | 6.09e-15 | 73.9 |
| MS.gene051373.t1 | MTR_8g005265 | 35.088 | 114 | 69 | 2 | 50 | 159 | 26 | 138 | 3.12e-14 | 72.0 |
| MS.gene051373.t1 | MTR_7g068160 | 39.450 | 109 | 58 | 3 | 50 | 150 | 35 | 143 | 5.04e-14 | 71.6 |
| MS.gene051373.t1 | MTR_4g009590 | 29.252 | 147 | 89 | 4 | 47 | 189 | 38 | 173 | 1.06e-13 | 70.9 |
| MS.gene051373.t1 | MTR_8g005235 | 33.628 | 113 | 72 | 2 | 51 | 160 | 27 | 139 | 1.52e-13 | 70.1 |
| MS.gene051373.t1 | MTR_4g009340 | 28.276 | 145 | 89 | 4 | 49 | 189 | 40 | 173 | 3.07e-13 | 69.3 |
| MS.gene051373.t1 | MTR_7g067970 | 33.108 | 148 | 90 | 4 | 50 | 192 | 28 | 171 | 5.67e-13 | 68.6 |
| MS.gene051373.t1 | MTR_8g005285 | 33.945 | 109 | 66 | 2 | 51 | 153 | 36 | 144 | 1.50e-12 | 67.4 |
| MS.gene051373.t1 | MTR_8g005325 | 35.088 | 114 | 69 | 3 | 51 | 160 | 56 | 168 | 1.84e-12 | 67.0 |
| MS.gene051373.t1 | MTR_8g005295 | 33.028 | 109 | 67 | 3 | 51 | 153 | 36 | 144 | 1.86e-12 | 67.0 |
| MS.gene051373.t1 | MTR_5g087570 | 36.000 | 75 | 48 | 0 | 52 | 126 | 28 | 102 | 2.02e-12 | 62.8 |
| MS.gene051373.t1 | MTR_8g042830 | 27.737 | 137 | 87 | 2 | 127 | 255 | 285 | 417 | 3.97e-12 | 65.9 |
| MS.gene051373.t1 | MTR_0209s0060 | 31.579 | 114 | 74 | 3 | 51 | 160 | 29 | 142 | 5.52e-12 | 65.5 |
| MS.gene051373.t1 | MTR_0209s0030 | 31.579 | 114 | 74 | 3 | 51 | 160 | 29 | 142 | 5.52e-12 | 65.5 |
| MS.gene051373.t1 | MTR_8g006525 | 32.456 | 114 | 72 | 3 | 51 | 160 | 28 | 140 | 5.71e-12 | 65.5 |
| MS.gene051373.t1 | MTR_4g015290 | 35.514 | 107 | 61 | 3 | 50 | 155 | 27 | 126 | 5.82e-12 | 63.9 |
| MS.gene051373.t1 | MTR_7g067990 | 35.593 | 118 | 68 | 3 | 50 | 159 | 23 | 140 | 7.60e-12 | 65.1 |
| MS.gene051373.t1 | MTR_4g009620 | 25.352 | 142 | 96 | 3 | 49 | 189 | 40 | 172 | 8.18e-12 | 65.1 |
| MS.gene051373.t1 | MTR_6g010850 | 25.767 | 163 | 112 | 1 | 91 | 253 | 197 | 350 | 1.01e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051373.t1 | AT2G36430 | 58.451 | 142 | 55 | 2 | 20 | 160 | 17 | 155 | 5.89e-50 | 170 |
| MS.gene051373.t1 | AT2G36430 | 54.128 | 109 | 50 | 0 | 147 | 255 | 338 | 446 | 2.46e-31 | 120 |
| MS.gene051373.t1 | AT3G47210 | 47.581 | 124 | 65 | 0 | 36 | 159 | 78 | 201 | 1.84e-32 | 124 |
| MS.gene051373.t1 | AT5G22540 | 44.444 | 144 | 73 | 3 | 23 | 160 | 1 | 143 | 3.65e-32 | 122 |
| MS.gene051373.t1 | AT5G22540 | 46.465 | 99 | 53 | 0 | 157 | 255 | 336 | 434 | 9.15e-20 | 88.2 |
| MS.gene051373.t1 | AT3G44700 | 43.210 | 162 | 77 | 5 | 7 | 160 | 11 | 165 | 5.12e-32 | 117 |
| MS.gene051373.t1 | AT3G47250 | 44.094 | 127 | 68 | 2 | 36 | 159 | 46 | 172 | 3.48e-31 | 120 |
| MS.gene051373.t1 | AT3G47250 | 28.226 | 124 | 83 | 2 | 133 | 255 | 358 | 476 | 6.37e-16 | 77.4 |
| MS.gene051373.t1 | AT3G47250 | 44.094 | 127 | 68 | 2 | 36 | 159 | 46 | 172 | 3.48e-31 | 120 |
| MS.gene051373.t1 | AT3G47250 | 28.226 | 124 | 83 | 2 | 133 | 255 | 358 | 476 | 6.37e-16 | 77.4 |
| MS.gene051373.t1 | AT3G47250 | 44.094 | 127 | 68 | 2 | 36 | 159 | 46 | 172 | 3.48e-31 | 120 |
| MS.gene051373.t1 | AT3G47250 | 28.226 | 124 | 83 | 2 | 133 | 255 | 358 | 476 | 6.37e-16 | 77.4 |
| MS.gene051373.t1 | AT5G22550 | 43.089 | 123 | 65 | 1 | 133 | 255 | 320 | 437 | 1.20e-30 | 118 |
| MS.gene051373.t1 | AT5G22550 | 50.000 | 68 | 32 | 1 | 39 | 104 | 32 | 99 | 5.60e-14 | 71.6 |
| MS.gene051373.t1 | AT3G44710 | 39.506 | 162 | 90 | 2 | 94 | 255 | 347 | 500 | 2.18e-30 | 118 |
| MS.gene051373.t1 | AT3G44710 | 46.281 | 121 | 61 | 2 | 39 | 155 | 35 | 155 | 2.79e-27 | 110 |
| MS.gene051373.t1 | AT5G22550 | 43.089 | 123 | 65 | 1 | 133 | 255 | 369 | 486 | 2.49e-30 | 118 |
| MS.gene051373.t1 | AT5G22550 | 42.520 | 127 | 68 | 2 | 39 | 160 | 32 | 158 | 3.59e-27 | 109 |
| MS.gene051373.t1 | AT3G47200 | 46.457 | 127 | 64 | 3 | 36 | 159 | 31 | 156 | 8.63e-29 | 114 |
| MS.gene051373.t1 | AT3G47200 | 32.000 | 125 | 79 | 2 | 133 | 256 | 341 | 460 | 9.97e-18 | 82.4 |
| MS.gene051373.t1 | AT3G47200 | 46.457 | 127 | 64 | 3 | 36 | 159 | 31 | 156 | 8.63e-29 | 114 |
| MS.gene051373.t1 | AT3G47200 | 32.000 | 125 | 79 | 2 | 133 | 256 | 341 | 460 | 9.97e-18 | 82.4 |
| MS.gene051373.t1 | AT5G22560 | 39.837 | 123 | 69 | 1 | 133 | 255 | 396 | 513 | 1.46e-28 | 113 |
| MS.gene051373.t1 | AT5G22560 | 39.726 | 146 | 70 | 3 | 33 | 160 | 28 | 173 | 1.25e-26 | 108 |
| MS.gene051373.t1 | AT5G11290 | 34.615 | 182 | 106 | 4 | 30 | 208 | 14 | 185 | 3.81e-27 | 108 |
| MS.gene051373.t1 | AT4G31980 | 45.614 | 114 | 62 | 0 | 40 | 153 | 282 | 395 | 9.36e-27 | 108 |
| MS.gene051373.t1 | AT3G50120 | 41.353 | 133 | 71 | 3 | 47 | 177 | 101 | 228 | 1.13e-26 | 108 |
| MS.gene051373.t1 | AT3G50120 | 39.394 | 99 | 60 | 0 | 157 | 255 | 429 | 527 | 2.96e-18 | 84.3 |
| MS.gene051373.t1 | AT3G50170 | 47.170 | 106 | 54 | 1 | 47 | 152 | 111 | 214 | 4.07e-26 | 107 |
| MS.gene051373.t1 | AT3G50170 | 36.634 | 101 | 64 | 0 | 155 | 255 | 435 | 535 | 1.27e-16 | 79.3 |
| MS.gene051373.t1 | AT3G50170 | 47.170 | 106 | 54 | 1 | 47 | 152 | 111 | 214 | 4.78e-26 | 105 |
| MS.gene051373.t1 | AT3G50130 | 45.545 | 101 | 55 | 0 | 52 | 152 | 140 | 240 | 2.80e-25 | 104 |
| MS.gene051373.t1 | AT3G50130 | 41.748 | 103 | 60 | 0 | 155 | 257 | 460 | 562 | 2.06e-12 | 67.0 |
| MS.gene051373.t1 | AT3G44700 | 43.307 | 127 | 61 | 4 | 7 | 129 | 11 | 130 | 1.63e-24 | 97.1 |
| MS.gene051373.t1 | AT3G50160 | 42.453 | 106 | 61 | 0 | 50 | 155 | 103 | 208 | 1.68e-24 | 102 |
| MS.gene051373.t1 | AT3G50160 | 40.594 | 101 | 60 | 0 | 155 | 255 | 397 | 497 | 9.19e-20 | 88.6 |
| MS.gene051373.t1 | AT3G50150 | 46.078 | 102 | 54 | 1 | 52 | 152 | 91 | 192 | 2.47e-24 | 101 |
| MS.gene051373.t1 | AT3G50150 | 39.604 | 101 | 61 | 0 | 155 | 255 | 405 | 505 | 1.10e-17 | 82.4 |
| MS.gene051373.t1 | AT3G50150 | 46.078 | 102 | 54 | 1 | 52 | 152 | 91 | 192 | 4.49e-24 | 100 |
| MS.gene051373.t1 | AT3G50180 | 45.455 | 110 | 58 | 1 | 45 | 154 | 174 | 281 | 1.16e-23 | 100 |
| MS.gene051373.t1 | AT3G50180 | 40.385 | 104 | 58 | 1 | 157 | 256 | 482 | 585 | 3.05e-17 | 81.3 |
| MS.gene051373.t1 | AT3G50190 | 41.584 | 101 | 59 | 0 | 52 | 152 | 97 | 197 | 1.37e-23 | 98.2 |
| MS.gene051373.t1 | AT3G50190 | 41.584 | 101 | 59 | 0 | 52 | 152 | 97 | 197 | 2.17e-23 | 97.8 |
| MS.gene051373.t1 | AT3G50190 | 41.584 | 101 | 59 | 0 | 52 | 152 | 97 | 197 | 2.17e-23 | 97.8 |
| MS.gene051373.t1 | AT3G50190 | 41.584 | 101 | 59 | 0 | 52 | 152 | 97 | 197 | 3.61e-23 | 98.2 |
| MS.gene051373.t1 | AT3G50140 | 40.594 | 101 | 60 | 0 | 52 | 152 | 114 | 214 | 1.02e-22 | 97.1 |
| MS.gene051373.t1 | AT3G50140 | 40.594 | 101 | 60 | 0 | 155 | 255 | 435 | 535 | 1.52e-20 | 90.9 |
| MS.gene051373.t1 | AT3G50140 | 40.594 | 101 | 60 | 0 | 52 | 152 | 114 | 214 | 1.05e-22 | 96.3 |
| MS.gene051373.t1 | AT5G11290 | 31.452 | 124 | 74 | 3 | 88 | 208 | 1 | 116 | 2.35e-12 | 66.2 |
Find 39 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGGATTTGCTTAGTAATTT+TGG | 0.067484 | 7.2:+8746553 | None:intergenic |
| AACATAGTTGAGAATCATTT+TGG | 0.141581 | 7.2:-8745576 | MS.gene051373:CDS |
| TTCTTCATTGTTGTGATTAT+TGG | 0.145321 | 7.2:+8746660 | None:intergenic |
| GACGAGGTTCATCAATATTA+TGG | 0.195531 | 7.2:-8745465 | MS.gene051373:CDS |
| GGCTTCCAAAACCTCTTCTA+AGG | 0.272835 | 7.2:+8746351 | None:intergenic |
| ATTACCCTTAGAAGAGGTTT+TGG | 0.319735 | 7.2:-8746356 | MS.gene051373:CDS |
| GAACATAAATATCGTTATCT+TGG | 0.354708 | 7.2:-8746400 | MS.gene051373:CDS |
| TTGGATATGTGTTATTTGTC+TGG | 0.356356 | 7.2:-8745495 | MS.gene051373:CDS |
| AATATCTTGTTGCATCTTCA+AGG | 0.359058 | 7.2:-8746524 | MS.gene051373:CDS |
| AAAAGATGTTGCGGTTGATT+TGG | 0.372070 | 7.2:-8745514 | MS.gene051373:CDS |
| TTGGTCGTTTATCTCTGCTT+TGG | 0.402449 | 7.2:-8745388 | MS.gene051373:CDS |
| ATCAATATTATGGAAATAGT+TGG | 0.410722 | 7.2:-8745455 | MS.gene051373:CDS |
| TATCTCTGCTTTGGCTGCAT+TGG | 0.413002 | 7.2:-8745379 | MS.gene051373:CDS |
| GAAATAGTTGGCATGTTCAA+TGG | 0.413194 | 7.2:-8745443 | MS.gene051373:CDS |
| GCACATTTGATTAATAATGC+TGG | 0.449997 | 7.2:-8745537 | MS.gene051373:CDS |
| TTCGTGTGAATCAAGTTGAA+TGG | 0.452910 | 7.2:+8746285 | None:intergenic |
| ACACGAATTCGTTGAAATGA+TGG | 0.453940 | 7.2:-8746269 | MS.gene051373:CDS |
| GTTGAAATGATGGTACTTGA+TGG | 0.462276 | 7.2:-8746259 | MS.gene051373:CDS |
| GTCTTGCTGATTCTCACGGT+TGG | 0.468434 | 7.2:-8745357 | MS.gene051373:CDS |
| TCCACGATGGTAAGGTCCTA+TGG | 0.474312 | 7.2:+8746447 | None:intergenic |
| AAATAGTTGGCATGTTCAAT+GGG | 0.499667 | 7.2:-8745442 | MS.gene051373:CDS |
| ATTGGTCTTGCTGATTCTCA+CGG | 0.512389 | 7.2:-8745361 | MS.gene051373:CDS |
| CGAGGTTGTCCACGATGGTA+AGG | 0.515482 | 7.2:+8746439 | None:intergenic |
| AACACAATTACCCTTAGAAG+AGG | 0.535365 | 7.2:-8746362 | MS.gene051373:CDS |
| TTTGCTTCAACGAAGGTTTG+TGG | 0.536234 | 7.2:+8746499 | None:intergenic |
| GCTTCCAAAACCTCTTCTAA+GGG | 0.545818 | 7.2:+8746352 | None:intergenic |
| TTTAACGTTTGCTTCAACGA+AGG | 0.547855 | 7.2:+8746492 | None:intergenic |
| ATGTTGAGTATTTATGTGAA+AGG | 0.553593 | 7.2:-8745599 | MS.gene051373:CDS |
| TTTGTCTGGTTTGTTTGACG+AGG | 0.614289 | 7.2:-8745481 | MS.gene051373:CDS |
| CACCCTCGTATAGTTTCCAT+AGG | 0.628917 | 7.2:-8746463 | MS.gene051373:CDS |
| AAATTACTAAGCAAATCCGC+AGG | 0.640596 | 7.2:-8746550 | MS.gene051373:CDS |
| TAATGCTGGAAAAGATGTTG+CGG | 0.662689 | 7.2:-8745523 | MS.gene051373:CDS |
| TCTTCGATCATGTTGAGACG+AGG | 0.666022 | 7.2:+8746421 | None:intergenic |
| GTCCTATGGAAACTATACGA+GGG | 0.676624 | 7.2:+8746461 | None:intergenic |
| TCCATAGGACCTTACCATCG+TGG | 0.683623 | 7.2:-8746448 | MS.gene051373:CDS |
| CTATGGAAACTATACGAGGG+TGG | 0.693319 | 7.2:+8746464 | None:intergenic |
| AAAGCAGAGATAAACGACCA+AGG | 0.693575 | 7.2:+8745390 | None:intergenic |
| GGTCCTATGGAAACTATACG+AGG | 0.715116 | 7.2:+8746460 | None:intergenic |
| TGAGACGAGGTTGTCCACGA+TGG | 0.728331 | 7.2:+8746434 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAATCTCTATAAAAAAA+TGG | + | chr7.2:8745839-8745858 | None:intergenic | 15.0% |
| !! | ACAATCTAATAATGTTACAT+AGG | + | chr7.2:8746346-8746365 | None:intergenic | 20.0% |
| !! | ATCAAAGAATTTACTTGATT+TGG | - | chr7.2:8746029-8746048 | MS.gene051373:intron | 20.0% |
| !! | ATCAATATTATGGAAATAGT+TGG | - | chr7.2:8746529-8746548 | MS.gene051373:CDS | 20.0% |
| !! | ATTGTTTGATCAATACTTAT+AGG | - | chr7.2:8746361-8746380 | MS.gene051373:CDS | 20.0% |
| !! | TTGTTTGATCAATACTTATA+GGG | - | chr7.2:8746362-8746381 | MS.gene051373:CDS | 20.0% |
| !!! | TCTTTTTCTTTTACACTAAT+CGG | + | chr7.2:8746075-8746094 | None:intergenic | 20.0% |
| ! | ATGTTGAGTATTTATGTGAA+AGG | - | chr7.2:8746385-8746404 | MS.gene051373:CDS | 25.0% |
| ! | GAACATAAATATCGTTATCT+TGG | - | chr7.2:8745584-8745603 | MS.gene051373:CDS | 25.0% |
| ! | TTCTTCATTGTTGTGATTAT+TGG | + | chr7.2:8745327-8745346 | None:intergenic | 25.0% |
| !! | AAATCATCCATTGTTATTGT+TGG | + | chr7.2:8746240-8746259 | None:intergenic | 25.0% |
| !! | AACATAGTTGAGAATCATTT+TGG | - | chr7.2:8746408-8746427 | MS.gene051373:CDS | 25.0% |
| !! | ACATAGTTGAGAATCATTTT+GGG | - | chr7.2:8746409-8746428 | MS.gene051373:CDS | 25.0% |
| !!! | ATACTTATTTTGATACTCCT+TGG | - | chr7.2:8746577-8746596 | MS.gene051373:CDS | 25.0% |
| AAATAGTTGGCATGTTCAAT+GGG | - | chr7.2:8746542-8746561 | MS.gene051373:CDS | 30.0% | |
| AACACTTTCATATCTTGCTA+TGG | - | chr7.2:8745939-8745958 | MS.gene051373:intron | 30.0% | |
| AATATCTTGTTGCATCTTCA+AGG | - | chr7.2:8745460-8745479 | MS.gene051373:CDS | 30.0% | |
| ACCACCAAATGAAAATTCAA+CGG | - | chr7.2:8745915-8745934 | MS.gene051373:intron | 30.0% | |
| GCACATTTGATTAATAATGC+TGG | - | chr7.2:8746447-8746466 | MS.gene051373:CDS | 30.0% | |
| TGAAATGCCAACAATAACAA+TGG | - | chr7.2:8746230-8746249 | MS.gene051373:CDS | 30.0% | |
| TTGAATGTGAAGTTCAAACA+CGG | - | chr7.2:8746201-8746220 | MS.gene051373:intron | 30.0% | |
| ! | GTTACCGTTGAATTTTCATT+TGG | + | chr7.2:8745922-8745941 | None:intergenic | 30.0% |
| ! | TTCGTTTTCTAATGGTGATA+TGG | + | chr7.2:8745657-8745676 | None:intergenic | 30.0% |
| ! | TTTTGCAGATATCTATCACA+TGG | + | chr7.2:8745364-8745383 | None:intergenic | 30.0% |
| !! | CGGATTTGCTTAGTAATTTT+GGG | + | chr7.2:8745433-8745452 | None:intergenic | 30.0% |
| !! | TTGGATATGTGTTATTTGTC+TGG | - | chr7.2:8746489-8746508 | MS.gene051373:CDS | 30.0% |
| AAAAGATGTTGCGGTTGATT+TGG | - | chr7.2:8746470-8746489 | MS.gene051373:CDS | 35.0% | |
| AAATTACTAAGCAAATCCGC+AGG | - | chr7.2:8745434-8745453 | MS.gene051373:CDS | 35.0% | |
| AACACAATTACCCTTAGAAG+AGG | - | chr7.2:8745622-8745641 | MS.gene051373:intron | 35.0% | |
| AAGTGGATCATCAACTTCAA+AGG | + | chr7.2:8745795-8745814 | None:intergenic | 35.0% | |
| ACACAATGAATTATGTGCGT+TGG | + | chr7.2:8746117-8746136 | None:intergenic | 35.0% | |
| ACACGAATTCGTTGAAATGA+TGG | - | chr7.2:8745715-8745734 | MS.gene051373:intron | 35.0% | |
| AGTGGATCATCAACTTCAAA+GGG | + | chr7.2:8745794-8745813 | None:intergenic | 35.0% | |
| AGTGGGATTAAGATAAATCC+AGG | - | chr7.2:8746153-8746172 | MS.gene051373:intron | 35.0% | |
| ATCATGATAACCTCTTCTTC+TGG | + | chr7.2:8745991-8746010 | None:intergenic | 35.0% | |
| GAAATAGTTGGCATGTTCAA+TGG | - | chr7.2:8746541-8746560 | MS.gene051373:CDS | 35.0% | |
| GACGAGGTTCATCAATATTA+TGG | - | chr7.2:8746519-8746538 | MS.gene051373:CDS | 35.0% | |
| GTGGGATTAAGATAAATCCA+GGG | - | chr7.2:8746154-8746173 | MS.gene051373:intron | 35.0% | |
| GTTGAAATGATGGTACTTGA+TGG | - | chr7.2:8745725-8745744 | MS.gene051373:intron | 35.0% | |
| TAATGCTGGAAAAGATGTTG+CGG | - | chr7.2:8746461-8746480 | MS.gene051373:CDS | 35.0% | |
| TTTAACGTTTGCTTCAACGA+AGG | + | chr7.2:8745495-8745514 | None:intergenic | 35.0% | |
| ! | ACCGTTGAATTTTCATTTGG+TGG | + | chr7.2:8745919-8745938 | None:intergenic | 35.0% |
| ! | ATTACCCTTAGAAGAGGTTT+TGG | - | chr7.2:8745628-8745647 | MS.gene051373:intron | 35.0% |
| ! | GCAACAAGATATTTTTCCTG+CGG | + | chr7.2:8745453-8745472 | None:intergenic | 35.0% |
| ! | GCTTTTGAACAATGTTACAG+TGG | - | chr7.2:8746288-8746307 | MS.gene051373:CDS | 35.0% |
| ! | TGATGATCCACTTGTTAACA+TGG | - | chr7.2:8745802-8745821 | MS.gene051373:intron | 35.0% |
| !! | AAGCGTTGAAGAACAAAGAA+AGG | + | chr7.2:8745881-8745900 | None:intergenic | 35.0% |
| !! | GCGGATTTGCTTAGTAATTT+TGG | + | chr7.2:8745434-8745453 | None:intergenic | 35.0% |
| !! | TCTCTAGCTTCGTTTTCTAA+TGG | + | chr7.2:8745665-8745684 | None:intergenic | 35.0% |
| !! | TTCGTGTGAATCAAGTTGAA+TGG | + | chr7.2:8745702-8745721 | None:intergenic | 35.0% |
| !!! | AACTTTTTCGCAAAGTTGCA+AGG | - | chr7.2:8745762-8745781 | MS.gene051373:intron | 35.0% |
| AAAGCAGAGATAAACGACCA+AGG | + | chr7.2:8746597-8746616 | None:intergenic | 40.0% | |
| ATCCATGCCATGTTAACAAG+TGG | + | chr7.2:8745812-8745831 | None:intergenic | 40.0% | |
| GCTTCCAAAACCTCTTCTAA+GGG | + | chr7.2:8745635-8745654 | None:intergenic | 40.0% | |
| GTCCTATGGAAACTATACGA+GGG | + | chr7.2:8745526-8745545 | None:intergenic | 40.0% | |
| TTGGTCGTTTATCTCTGCTT+TGG | - | chr7.2:8746596-8746615 | MS.gene051373:CDS | 40.0% | |
| TTTGCTTCAACGAAGGTTTG+TGG | + | chr7.2:8745488-8745507 | None:intergenic | 40.0% | |
| ! | ATCCACTTGTTAACATGGCA+TGG | - | chr7.2:8745807-8745826 | MS.gene051373:intron | 40.0% |
| ! | ATGAATTATGTGCGTTGGTG+TGG | + | chr7.2:8746112-8746131 | None:intergenic | 40.0% |
| !! | ATTGGTCTTGCTGATTCTCA+CGG | - | chr7.2:8746623-8746642 | MS.gene051373:CDS | 40.0% |
| !! | TTTGTCTGGTTTGTTTGACG+AGG | - | chr7.2:8746503-8746522 | MS.gene051373:CDS | 40.0% |
| !!! | GAGAATCATTTTGGGACAGA+AGG | - | chr7.2:8746417-8746436 | MS.gene051373:CDS | 40.0% |
| !!! | TTTTGGGACAGAAGGTGAAA+TGG | - | chr7.2:8746425-8746444 | MS.gene051373:CDS | 40.0% |
| CACCCTCGTATAGTTTCCAT+AGG | - | chr7.2:8745521-8745540 | MS.gene051373:CDS | 45.0% | |
| CTATGGAAACTATACGAGGG+TGG | + | chr7.2:8745523-8745542 | None:intergenic | 45.0% | |
| GCTACAAAGACCAGAAGAAG+AGG | - | chr7.2:8745978-8745997 | MS.gene051373:intron | 45.0% | |
| GGCTTCCAAAACCTCTTCTA+AGG | + | chr7.2:8745636-8745655 | None:intergenic | 45.0% | |
| GGTCCTATGGAAACTATACG+AGG | + | chr7.2:8745527-8745546 | None:intergenic | 45.0% | |
| TAACAAAGCTTCGTCGCAGT+GGG | - | chr7.2:8746136-8746155 | MS.gene051373:intron | 45.0% | |
| TATCTCTGCTTTGGCTGCAT+TGG | - | chr7.2:8746605-8746624 | MS.gene051373:CDS | 45.0% | |
| TCTTCGATCATGTTGAGACG+AGG | + | chr7.2:8745566-8745585 | None:intergenic | 45.0% | |
| !!! | TCTCTTTCGTTGCTTTTCCC+TGG | + | chr7.2:8746174-8746193 | None:intergenic | 45.0% |
| GTAACAAAGCTTCGTCGCAG+TGG | - | chr7.2:8746135-8746154 | MS.gene051373:intron | 50.0% | |
| TCCACGATGGTAAGGTCCTA+TGG | + | chr7.2:8745540-8745559 | None:intergenic | 50.0% | |
| TCCATAGGACCTTACCATCG+TGG | - | chr7.2:8745536-8745555 | MS.gene051373:CDS | 50.0% | |
| !! | GTCTTGCTGATTCTCACGGT+TGG | - | chr7.2:8746627-8746646 | MS.gene051373:CDS | 50.0% |
| CGAGGTTGTCCACGATGGTA+AGG | + | chr7.2:8745548-8745567 | None:intergenic | 55.0% | |
| TGAGACGAGGTTGTCCACGA+TGG | + | chr7.2:8745553-8745572 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 8745317 | 8746689 | 8745317 | ID=MS.gene051373 |
| chr7.2 | mRNA | 8745317 | 8746689 | 8745317 | ID=MS.gene051373.t1;Parent=MS.gene051373 |
| chr7.2 | exon | 8746223 | 8746689 | 8746223 | ID=MS.gene051373.t1.exon1;Parent=MS.gene051373.t1 |
| chr7.2 | CDS | 8746223 | 8746689 | 8746223 | ID=cds.MS.gene051373.t1;Parent=MS.gene051373.t1 |
| chr7.2 | exon | 8745317 | 8745623 | 8745317 | ID=MS.gene051373.t1.exon2;Parent=MS.gene051373.t1 |
| chr7.2 | CDS | 8745317 | 8745623 | 8745317 | ID=cds.MS.gene051373.t1;Parent=MS.gene051373.t1 |
| Gene Sequence |
| Protein sequence |
