Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08815.t1 | XP_003629603.1 | 98.3 | 174 | 3 | 0 | 1 | 174 | 1 | 174 | 3.20E-75 | 291.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08815.t1 | G7LH55 | 98.3 | 174 | 3 | 0 | 1 | 174 | 1 | 174 | 2.3e-75 | 291.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049133 | MS.gene08815 | 0.80546 | 1.32E-49 | -1.69E-46 |
| MS.gene049418 | MS.gene08815 | 0.859783 | 3.26E-63 | -1.69E-46 |
| MS.gene049989 | MS.gene08815 | 0.861698 | 8.55E-64 | -1.69E-46 |
| MS.gene050297 | MS.gene08815 | 0.878227 | 3.33E-69 | -1.69E-46 |
| MS.gene050310 | MS.gene08815 | 0.809406 | 1.92E-50 | -1.69E-46 |
| MS.gene051263 | MS.gene08815 | 0.825736 | 3.98E-54 | -1.69E-46 |
| MS.gene051478 | MS.gene08815 | 0.834118 | 3.61E-56 | -1.69E-46 |
| MS.gene051648 | MS.gene08815 | 0.800279 | 1.55E-48 | -1.69E-46 |
| MS.gene052143 | MS.gene08815 | 0.846668 | 1.88E-59 | -1.69E-46 |
| MS.gene052367 | MS.gene08815 | 0.838499 | 2.77E-57 | -1.69E-46 |
| MS.gene053416 | MS.gene08815 | 0.8092 | 2.13E-50 | -1.69E-46 |
| MS.gene053417 | MS.gene08815 | 0.80705 | 6.11E-50 | -1.69E-46 |
| MS.gene05369 | MS.gene08815 | 0.80203 | 6.81E-49 | -1.69E-46 |
| MS.gene054643 | MS.gene08815 | 0.810781 | 9.72E-51 | -1.69E-46 |
| MS.gene055957 | MS.gene08815 | 0.831909 | 1.28E-55 | -1.69E-46 |
| MS.gene056041 | MS.gene08815 | 0.835055 | 2.10E-56 | -1.69E-46 |
| MS.gene056042 | MS.gene08815 | 0.828725 | 7.66E-55 | -1.69E-46 |
| MS.gene056526 | MS.gene08815 | 0.824868 | 6.39E-54 | -1.69E-46 |
| MS.gene057004 | MS.gene08815 | 0.805138 | 1.54E-49 | -1.69E-46 |
| MS.gene057483 | MS.gene08815 | 0.82218 | 2.72E-53 | -1.69E-46 |
| MS.gene058196 | MS.gene08815 | 0.848872 | 4.65E-60 | -1.69E-46 |
| MS.gene058197 | MS.gene08815 | 0.859087 | 5.27E-63 | -1.69E-46 |
| MS.gene058198 | MS.gene08815 | 0.854942 | 8.80E-62 | -1.69E-46 |
| MS.gene059300 | MS.gene08815 | 0.84894 | 4.45E-60 | -1.69E-46 |
| MS.gene060473 | MS.gene08815 | 0.81782 | 2.70E-52 | -1.69E-46 |
| MS.gene060565 | MS.gene08815 | 0.840437 | 8.70E-58 | -1.69E-46 |
| MS.gene06057 | MS.gene08815 | 0.881705 | 1.92E-70 | -1.69E-46 |
| MS.gene061099 | MS.gene08815 | 0.802805 | 4.71E-49 | -1.69E-46 |
| MS.gene061260 | MS.gene08815 | 0.842115 | 3.15E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08815.t1 | MTR_8g081530 | 98.276 | 174 | 3 | 0 | 1 | 174 | 1 | 174 | 3.06e-124 | 347 |
| MS.gene08815.t1 | MTR_7g080550 | 70.857 | 175 | 44 | 2 | 4 | 174 | 14 | 185 | 1.77e-81 | 239 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08815.t1 | AT5G16250 | 66.667 | 174 | 55 | 2 | 3 | 174 | 11 | 183 | 1.19e-71 | 214 |
| MS.gene08815.t1 | AT3G02640 | 62.286 | 175 | 62 | 2 | 3 | 174 | 12 | 185 | 9.52e-66 | 199 |
| MS.gene08815.t1 | AT5G36800 | 59.302 | 172 | 65 | 2 | 3 | 169 | 4 | 175 | 8.04e-61 | 187 |
| MS.gene08815.t1 | AT5G36710 | 59.302 | 172 | 65 | 2 | 3 | 169 | 4 | 175 | 8.04e-61 | 187 |
| MS.gene08815.t1 | AT5G36800 | 59.649 | 171 | 64 | 2 | 4 | 169 | 40 | 210 | 1.22e-60 | 187 |
| MS.gene08815.t1 | AT5G36710 | 59.649 | 171 | 64 | 2 | 4 | 169 | 40 | 210 | 1.22e-60 | 187 |
Find 37 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACACCATCACCACCTTCT+TGG | 0.333498 | 4.2:+13136441 | None:intergenic |
| TCAGAATGAAGAAGATTTGA+AGG | 0.365892 | 4.2:-13136402 | MS.gene08815:CDS |
| AGAAACTGAACCTTGAAATA+TGG | 0.377833 | 4.2:+13136631 | None:intergenic |
| GTGCTAACTGCCATATTTCA+AGG | 0.401955 | 4.2:-13136641 | MS.gene08815:CDS |
| ATATTGAAGGGAAAAGGTTA+AGG | 0.429185 | 4.2:+13136780 | None:intergenic |
| ATGCTTTGTGAATGTTGTAT+TGG | 0.433769 | 4.2:+13136864 | None:intergenic |
| ATGAAGAAGATTTGAAGGAT+TGG | 0.450129 | 4.2:-13136397 | MS.gene08815:CDS |
| TGCCACATATTGAAGGGAAA+AGG | 0.478974 | 4.2:+13136774 | None:intergenic |
| AAAGGTTAAGGGAGAGAGTG+AGG | 0.489342 | 4.2:+13136792 | None:intergenic |
| TATTGAAGGGAAAAGGTTAA+GGG | 0.490541 | 4.2:+13136781 | None:intergenic |
| AAGAAGATGCCACATATTGA+AGG | 0.497178 | 4.2:+13136767 | None:intergenic |
| TGCAGAGGCGGAAACAGCTC+CGG | 0.500608 | 4.2:+13136718 | None:intergenic |
| AAGTGTTATTCTTACCAAGA+AGG | 0.514174 | 4.2:-13136455 | MS.gene08815:CDS |
| CTCCGGCGATGGTGAATGCA+TGG | 0.521359 | 4.2:+13136735 | None:intergenic |
| GGCGGAAACAGCTCCGGCGA+TGG | 0.531024 | 4.2:+13136724 | None:intergenic |
| TGCAACTTGAGGATGCAGAT+AGG | 0.536458 | 4.2:+13136819 | None:intergenic |
| CTCCATGCATTCACCATCGC+CGG | 0.537447 | 4.2:-13136737 | MS.gene08815:CDS |
| TCTTACCAAGAAGGTGGTGA+TGG | 0.553959 | 4.2:-13136446 | MS.gene08815:CDS |
| CAATAACCACTCTTACTCGT+TGG | 0.578838 | 4.2:-13136688 | MS.gene08815:CDS |
| TGGTGTTATGAGAAGTGGTA+AGG | 0.590308 | 4.2:-13136426 | MS.gene08815:CDS |
| TCCTATGTGAGGGAAGAAGA+TGG | 0.593105 | 4.2:-13136563 | MS.gene08815:CDS |
| AGAAGATGCCACATATTGAA+GGG | 0.602971 | 4.2:+13136768 | None:intergenic |
| TCGTTGGTACTCTGCACACA+TGG | 0.604168 | 4.2:-13136672 | MS.gene08815:CDS |
| TCTGTTATATTGAAACTATG+TGG | 0.612123 | 4.2:-13136539 | MS.gene08815:CDS |
| ACCATCTTCTTCCCTCACAT+AGG | 0.616023 | 4.2:+13136562 | None:intergenic |
| AGTGAGGAAGATGCAACTTG+AGG | 0.621213 | 4.2:+13136808 | None:intergenic |
| GTTATATTGAAACTATGTGG+TGG | 0.623425 | 4.2:-13136536 | MS.gene08815:CDS |
| TGTTATTCTTACCAAGAAGG+TGG | 0.624748 | 4.2:-13136452 | MS.gene08815:CDS |
| AACTAAAGCTGTAAAGACAA+TGG | 0.629500 | 4.2:+13136374 | None:intergenic |
| GGTGATGGTGTTATGAGAAG+TGG | 0.642861 | 4.2:-13136431 | MS.gene08815:CDS |
| TTGAGTTGAAATCCTATGTG+AGG | 0.659596 | 4.2:-13136574 | MS.gene08815:CDS |
| AGAGTACCAACGAGTAAGAG+TGG | 0.670822 | 4.2:+13136682 | None:intergenic |
| TTGGTACTCTGCACACATGG+TGG | 0.684738 | 4.2:-13136669 | MS.gene08815:CDS |
| TGAGTTGAAATCCTATGTGA+GGG | 0.688051 | 4.2:-13136573 | MS.gene08815:CDS |
| TATTGATGATGATGCAGAGG+CGG | 0.695458 | 4.2:+13136706 | None:intergenic |
| GGTTATTGATGATGATGCAG+AGG | 0.698569 | 4.2:+13136703 | None:intergenic |
| CGGCGATGGTGAATGCATGG+AGG | 0.710944 | 4.2:+13136738 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTATTTTAATATTTTTGT+TGG | - | chr4.2:13136744-13136763 | MS.gene08815:CDS | 10.0% |
| !!! | TTTAATATTTTTGTTGGAGT+GGG | - | chr4.2:13136750-13136769 | MS.gene08815:CDS | 20.0% |
| !!! | TTTTAATATTTTTGTTGGAG+TGG | - | chr4.2:13136749-13136768 | MS.gene08815:CDS | 20.0% |
| ! | TCTGTTATATTGAAACTATG+TGG | - | chr4.2:13136715-13136734 | MS.gene08815:CDS | 25.0% |
| AGAAACTGAACCTTGAAATA+TGG | + | chr4.2:13136626-13136645 | None:intergenic | 30.0% | |
| ATATTGAAGGGAAAAGGTTA+AGG | + | chr4.2:13136477-13136496 | None:intergenic | 30.0% | |
| ATGAAGAAGATTTGAAGGAT+TGG | - | chr4.2:13136857-13136876 | MS.gene08815:CDS | 30.0% | |
| ATGCTTTGTGAATGTTGTAT+TGG | + | chr4.2:13136393-13136412 | None:intergenic | 30.0% | |
| GTTATATTGAAACTATGTGG+TGG | - | chr4.2:13136718-13136737 | MS.gene08815:CDS | 30.0% | |
| TATTGAAGGGAAAAGGTTAA+GGG | + | chr4.2:13136476-13136495 | None:intergenic | 30.0% | |
| TCAGAATGAAGAAGATTTGA+AGG | - | chr4.2:13136852-13136871 | MS.gene08815:CDS | 30.0% | |
| ! | AAGTGTTATTCTTACCAAGA+AGG | - | chr4.2:13136799-13136818 | MS.gene08815:CDS | 30.0% |
| AAGAAGATGCCACATATTGA+AGG | + | chr4.2:13136490-13136509 | None:intergenic | 35.0% | |
| AGAAGATGCCACATATTGAA+GGG | + | chr4.2:13136489-13136508 | None:intergenic | 35.0% | |
| TGTTATTCTTACCAAGAAGG+TGG | - | chr4.2:13136802-13136821 | MS.gene08815:CDS | 35.0% | |
| ! | AACCTTTTCCCTTCAATATG+TGG | - | chr4.2:13136478-13136497 | MS.gene08815:CDS | 35.0% |
| ! | TGAGTTGAAATCCTATGTGA+GGG | - | chr4.2:13136681-13136700 | MS.gene08815:CDS | 35.0% |
| !! | TTGAGTTGAAATCCTATGTG+AGG | - | chr4.2:13136680-13136699 | MS.gene08815:CDS | 35.0% |
| CAATAACCACTCTTACTCGT+TGG | - | chr4.2:13136566-13136585 | MS.gene08815:CDS | 40.0% | |
| GTGCTAACTGCCATATTTCA+AGG | - | chr4.2:13136613-13136632 | MS.gene08815:CDS | 40.0% | |
| TGCCACATATTGAAGGGAAA+AGG | + | chr4.2:13136483-13136502 | None:intergenic | 40.0% | |
| !! | GCTGATTTTCACAAGAACTG+AGG | - | chr4.2:13136648-13136667 | MS.gene08815:CDS | 40.0% |
| !! | GGTTATTGATGATGATGCAG+AGG | + | chr4.2:13136554-13136573 | None:intergenic | 40.0% |
| !! | TATTGATGATGATGCAGAGG+CGG | + | chr4.2:13136551-13136570 | None:intergenic | 40.0% |
| !! | TGGTGTTATGAGAAGTGGTA+AGG | - | chr4.2:13136828-13136847 | MS.gene08815:CDS | 40.0% |
| AAAGGTTAAGGGAGAGAGTG+AGG | + | chr4.2:13136465-13136484 | None:intergenic | 45.0% | |
| ACCATCTTCTTCCCTCACAT+AGG | + | chr4.2:13136695-13136714 | None:intergenic | 45.0% | |
| AGTGAGGAAGATGCAACTTG+AGG | + | chr4.2:13136449-13136468 | None:intergenic | 45.0% | |
| TAACACCATCACCACCTTCT+TGG | + | chr4.2:13136816-13136835 | None:intergenic | 45.0% | |
| TCCTATGTGAGGGAAGAAGA+TGG | - | chr4.2:13136691-13136710 | MS.gene08815:CDS | 45.0% | |
| TCTTACCAAGAAGGTGGTGA+TGG | - | chr4.2:13136808-13136827 | MS.gene08815:CDS | 45.0% | |
| TGCAACTTGAGGATGCAGAT+AGG | + | chr4.2:13136438-13136457 | None:intergenic | 45.0% | |
| ! | AGAGTACCAACGAGTAAGAG+TGG | + | chr4.2:13136575-13136594 | None:intergenic | 45.0% |
| !! | GGTGATGGTGTTATGAGAAG+TGG | - | chr4.2:13136823-13136842 | MS.gene08815:CDS | 45.0% |
| TCGTTGGTACTCTGCACACA+TGG | - | chr4.2:13136582-13136601 | MS.gene08815:CDS | 50.0% | |
| TTGGTACTCTGCACACATGG+TGG | - | chr4.2:13136585-13136604 | MS.gene08815:CDS | 50.0% | |
| CTCCATGCATTCACCATCGC+CGG | - | chr4.2:13136517-13136536 | MS.gene08815:CDS | 55.0% | |
| CGGCGATGGTGAATGCATGG+AGG | + | chr4.2:13136519-13136538 | None:intergenic | 60.0% | |
| CTCCGGCGATGGTGAATGCA+TGG | + | chr4.2:13136522-13136541 | None:intergenic | 60.0% | |
| TGCAGAGGCGGAAACAGCTC+CGG | + | chr4.2:13136539-13136558 | None:intergenic | 60.0% | |
| GGCGGAAACAGCTCCGGCGA+TGG | + | chr4.2:13136533-13136552 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 13136376 | 13136900 | 13136376 | ID=MS.gene08815 |
| chr4.2 | mRNA | 13136376 | 13136900 | 13136376 | ID=MS.gene08815.t1;Parent=MS.gene08815 |
| chr4.2 | exon | 13136376 | 13136900 | 13136376 | ID=MS.gene08815.t1.exon1;Parent=MS.gene08815.t1 |
| chr4.2 | CDS | 13136376 | 13136900 | 13136376 | ID=cds.MS.gene08815.t1;Parent=MS.gene08815.t1 |
| Gene Sequence |
| Protein sequence |
