Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08971.t1 | XP_003629603.1 | 97.7 | 174 | 4 | 0 | 1 | 174 | 1 | 174 | 2.70E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08971.t1 | G7LH55 | 97.7 | 174 | 4 | 0 | 1 | 174 | 1 | 174 | 1.9e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049133 | MS.gene08971 | 0.806094 | 9.72E-50 | -1.69E-46 |
| MS.gene049397 | MS.gene08971 | 0.800113 | 1.68E-48 | -1.69E-46 |
| MS.gene049418 | MS.gene08971 | 0.857388 | 1.69E-62 | -1.69E-46 |
| MS.gene049989 | MS.gene08971 | 0.859959 | 2.88E-63 | -1.69E-46 |
| MS.gene050297 | MS.gene08971 | 0.883928 | 2.96E-71 | -1.69E-46 |
| MS.gene050310 | MS.gene08971 | 0.82981 | 4.18E-55 | -1.69E-46 |
| MS.gene051263 | MS.gene08971 | 0.810672 | 1.03E-50 | -1.69E-46 |
| MS.gene051478 | MS.gene08971 | 0.832926 | 7.15E-56 | -1.69E-46 |
| MS.gene052143 | MS.gene08971 | 0.838492 | 2.78E-57 | -1.69E-46 |
| MS.gene052367 | MS.gene08971 | 0.836946 | 6.95E-57 | -1.69E-46 |
| MS.gene053416 | MS.gene08971 | 0.804383 | 2.22E-49 | -1.69E-46 |
| MS.gene053417 | MS.gene08971 | 0.803267 | 3.78E-49 | -1.69E-46 |
| MS.gene05369 | MS.gene08971 | 0.80729 | 5.43E-50 | -1.69E-46 |
| MS.gene054643 | MS.gene08971 | 0.817412 | 3.34E-52 | -1.69E-46 |
| MS.gene055957 | MS.gene08971 | 0.815348 | 9.68E-52 | -1.69E-46 |
| MS.gene056041 | MS.gene08971 | 0.825528 | 4.46E-54 | -1.69E-46 |
| MS.gene056042 | MS.gene08971 | 0.801633 | 8.21E-49 | -1.69E-46 |
| MS.gene056526 | MS.gene08971 | 0.812712 | 3.69E-51 | -1.69E-46 |
| MS.gene057004 | MS.gene08971 | 0.805138 | 1.54E-49 | -1.69E-46 |
| MS.gene05709 | MS.gene08971 | 0.803816 | 2.91E-49 | -1.69E-46 |
| MS.gene057483 | MS.gene08971 | 0.830864 | 2.31E-55 | -1.69E-46 |
| MS.gene058196 | MS.gene08971 | 0.845409 | 4.13E-59 | -1.69E-46 |
| MS.gene058197 | MS.gene08971 | 0.85754 | 1.52E-62 | -1.69E-46 |
| MS.gene058198 | MS.gene08971 | 0.858277 | 9.20E-63 | -1.69E-46 |
| MS.gene059300 | MS.gene08971 | 0.854168 | 1.47E-61 | -1.69E-46 |
| MS.gene060473 | MS.gene08971 | 0.815307 | 9.88E-52 | -1.69E-46 |
| MS.gene060565 | MS.gene08971 | 0.836344 | 9.89E-57 | -1.69E-46 |
| MS.gene06057 | MS.gene08971 | 0.885961 | 5.17E-72 | -1.69E-46 |
| MS.gene061260 | MS.gene08971 | 0.834461 | 2.96E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08971.t1 | MTR_8g081530 | 97.701 | 174 | 4 | 0 | 1 | 174 | 1 | 174 | 8.15e-123 | 343 |
| MS.gene08971.t1 | MTR_7g080550 | 70.857 | 175 | 44 | 2 | 4 | 174 | 14 | 185 | 2.94e-81 | 238 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08971.t1 | AT5G16250 | 66.092 | 174 | 56 | 2 | 3 | 174 | 11 | 183 | 9.72e-71 | 212 |
| MS.gene08971.t1 | AT3G02640 | 62.286 | 175 | 62 | 3 | 3 | 174 | 12 | 185 | 1.82e-64 | 196 |
| MS.gene08971.t1 | AT5G36800 | 59.302 | 172 | 65 | 2 | 3 | 169 | 4 | 175 | 1.15e-60 | 186 |
| MS.gene08971.t1 | AT5G36710 | 59.302 | 172 | 65 | 2 | 3 | 169 | 4 | 175 | 1.15e-60 | 186 |
| MS.gene08971.t1 | AT5G36800 | 59.649 | 171 | 64 | 2 | 4 | 169 | 40 | 210 | 1.24e-60 | 187 |
| MS.gene08971.t1 | AT5G36710 | 59.649 | 171 | 64 | 2 | 4 | 169 | 40 | 210 | 1.24e-60 | 187 |
Find 37 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACACCATCACCACCTTCT+TGG | 0.335671 | 4.4:+14849101 | None:intergenic |
| TCAGAATGAAGAAGATTTGA+AGG | 0.365892 | 4.4:-14849062 | MS.gene08971:CDS |
| AGAAACTGAACCTTGAAATA+TGG | 0.377833 | 4.4:+14849291 | None:intergenic |
| GTGCTAACTGCCATATTTCA+AGG | 0.401955 | 4.4:-14849301 | MS.gene08971:CDS |
| ATATTGAAGGGAAAAGGTTA+AGG | 0.429185 | 4.4:+14849440 | None:intergenic |
| ATGCTTTGTGAATGTTGTAT+TGG | 0.433769 | 4.4:+14849524 | None:intergenic |
| ATGAAGAAGATTTGAAGGAT+TGG | 0.450129 | 4.4:-14849057 | MS.gene08971:CDS |
| TGCCACATATTGAAGGGAAA+AGG | 0.478974 | 4.4:+14849434 | None:intergenic |
| AAAGGTTAAGGGAGAGAGTG+AGG | 0.489342 | 4.4:+14849452 | None:intergenic |
| TATTGAAGGGAAAAGGTTAA+GGG | 0.490541 | 4.4:+14849441 | None:intergenic |
| AAGAAGATGCCACATATTGA+AGG | 0.497178 | 4.4:+14849427 | None:intergenic |
| TGCAGAGGCGGAAACAGCTC+CGG | 0.500608 | 4.4:+14849378 | None:intergenic |
| AAGTGTTATTCTTACCAAGA+AGG | 0.514174 | 4.4:-14849115 | MS.gene08971:CDS |
| CTCCGGCGATGGTGAATGCA+TGG | 0.521359 | 4.4:+14849395 | None:intergenic |
| GGCGGAAACAGCTCCGGCGA+TGG | 0.531024 | 4.4:+14849384 | None:intergenic |
| TGCAACTTGAGGATGCAGAT+AGG | 0.536458 | 4.4:+14849479 | None:intergenic |
| CTCCATGCATTCACCATCGC+CGG | 0.537447 | 4.4:-14849397 | MS.gene08971:CDS |
| GGTGATGGTGTTATGATAAG+TGG | 0.550289 | 4.4:-14849091 | MS.gene08971:CDS |
| TCTTACCAAGAAGGTGGTGA+TGG | 0.553959 | 4.4:-14849106 | MS.gene08971:CDS |
| CAATAACCACTCTTACTCGT+TGG | 0.578838 | 4.4:-14849348 | MS.gene08971:CDS |
| TCCTATGTGAGGGAAGAAGA+TGG | 0.593105 | 4.4:-14849223 | MS.gene08971:CDS |
| AGAAGATGCCACATATTGAA+GGG | 0.602971 | 4.4:+14849428 | None:intergenic |
| TCGTTGGTACTCTGCACACA+TGG | 0.604168 | 4.4:-14849332 | MS.gene08971:CDS |
| TCTGTTATATTGAAACTATG+TGG | 0.612123 | 4.4:-14849199 | MS.gene08971:CDS |
| ACCATCTTCTTCCCTCACAT+AGG | 0.616023 | 4.4:+14849222 | None:intergenic |
| AGTGAGGAAGATGCAACTTG+AGG | 0.621213 | 4.4:+14849468 | None:intergenic |
| TGGTGTTATGATAAGTGGTA+AGG | 0.622843 | 4.4:-14849086 | MS.gene08971:CDS |
| GTTATATTGAAACTATGTGG+TGG | 0.623425 | 4.4:-14849196 | MS.gene08971:CDS |
| TGTTATTCTTACCAAGAAGG+TGG | 0.624748 | 4.4:-14849112 | MS.gene08971:CDS |
| AACTAAAGCTGTAAAGACAA+TGG | 0.629500 | 4.4:+14849034 | None:intergenic |
| TTGAGTTGAAATCCTATGTG+AGG | 0.659596 | 4.4:-14849234 | MS.gene08971:CDS |
| AGAGTACCAACGAGTAAGAG+TGG | 0.670822 | 4.4:+14849342 | None:intergenic |
| TTGGTACTCTGCACACATGG+TGG | 0.684738 | 4.4:-14849329 | MS.gene08971:CDS |
| TGAGTTGAAATCCTATGTGA+GGG | 0.688051 | 4.4:-14849233 | MS.gene08971:CDS |
| TATTGATGATGATGCAGAGG+CGG | 0.695458 | 4.4:+14849366 | None:intergenic |
| GGTTATTGATGATGATGCAG+AGG | 0.698569 | 4.4:+14849363 | None:intergenic |
| CGGCGATGGTGAATGCATGG+AGG | 0.710944 | 4.4:+14849398 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTATTTTAATATTTTTGT+TGG | - | chr4.4:14849404-14849423 | MS.gene08971:CDS | 10.0% |
| !!! | TTTAATATTTTTGTTGGAGT+GGG | - | chr4.4:14849410-14849429 | MS.gene08971:CDS | 20.0% |
| !!! | TTTTAATATTTTTGTTGGAG+TGG | - | chr4.4:14849409-14849428 | MS.gene08971:CDS | 20.0% |
| ! | TCTGTTATATTGAAACTATG+TGG | - | chr4.4:14849375-14849394 | MS.gene08971:CDS | 25.0% |
| AGAAACTGAACCTTGAAATA+TGG | + | chr4.4:14849286-14849305 | None:intergenic | 30.0% | |
| ATATTGAAGGGAAAAGGTTA+AGG | + | chr4.4:14849137-14849156 | None:intergenic | 30.0% | |
| ATGAAGAAGATTTGAAGGAT+TGG | - | chr4.4:14849517-14849536 | MS.gene08971:CDS | 30.0% | |
| ATGCTTTGTGAATGTTGTAT+TGG | + | chr4.4:14849053-14849072 | None:intergenic | 30.0% | |
| GTTATATTGAAACTATGTGG+TGG | - | chr4.4:14849378-14849397 | MS.gene08971:CDS | 30.0% | |
| TATTGAAGGGAAAAGGTTAA+GGG | + | chr4.4:14849136-14849155 | None:intergenic | 30.0% | |
| TCAGAATGAAGAAGATTTGA+AGG | - | chr4.4:14849512-14849531 | MS.gene08971:CDS | 30.0% | |
| ! | AAGTGTTATTCTTACCAAGA+AGG | - | chr4.4:14849459-14849478 | MS.gene08971:CDS | 30.0% |
| AAGAAGATGCCACATATTGA+AGG | + | chr4.4:14849150-14849169 | None:intergenic | 35.0% | |
| AGAAGATGCCACATATTGAA+GGG | + | chr4.4:14849149-14849168 | None:intergenic | 35.0% | |
| TGTTATTCTTACCAAGAAGG+TGG | - | chr4.4:14849462-14849481 | MS.gene08971:CDS | 35.0% | |
| ! | AACCTTTTCCCTTCAATATG+TGG | - | chr4.4:14849138-14849157 | MS.gene08971:CDS | 35.0% |
| ! | TGAGTTGAAATCCTATGTGA+GGG | - | chr4.4:14849341-14849360 | MS.gene08971:CDS | 35.0% |
| !! | TGGTGTTATGATAAGTGGTA+AGG | - | chr4.4:14849488-14849507 | MS.gene08971:CDS | 35.0% |
| !! | TTGAGTTGAAATCCTATGTG+AGG | - | chr4.4:14849340-14849359 | MS.gene08971:CDS | 35.0% |
| CAATAACCACTCTTACTCGT+TGG | - | chr4.4:14849226-14849245 | MS.gene08971:CDS | 40.0% | |
| GTGCTAACTGCCATATTTCA+AGG | - | chr4.4:14849273-14849292 | MS.gene08971:CDS | 40.0% | |
| TGCCACATATTGAAGGGAAA+AGG | + | chr4.4:14849143-14849162 | None:intergenic | 40.0% | |
| !! | GCTGATTTTCACAAGAACTG+AGG | - | chr4.4:14849308-14849327 | MS.gene08971:CDS | 40.0% |
| !! | GGTGATGGTGTTATGATAAG+TGG | - | chr4.4:14849483-14849502 | MS.gene08971:CDS | 40.0% |
| !! | GGTTATTGATGATGATGCAG+AGG | + | chr4.4:14849214-14849233 | None:intergenic | 40.0% |
| !! | TATTGATGATGATGCAGAGG+CGG | + | chr4.4:14849211-14849230 | None:intergenic | 40.0% |
| AAAGGTTAAGGGAGAGAGTG+AGG | + | chr4.4:14849125-14849144 | None:intergenic | 45.0% | |
| ACCATCTTCTTCCCTCACAT+AGG | + | chr4.4:14849355-14849374 | None:intergenic | 45.0% | |
| AGTGAGGAAGATGCAACTTG+AGG | + | chr4.4:14849109-14849128 | None:intergenic | 45.0% | |
| TAACACCATCACCACCTTCT+TGG | + | chr4.4:14849476-14849495 | None:intergenic | 45.0% | |
| TCCTATGTGAGGGAAGAAGA+TGG | - | chr4.4:14849351-14849370 | MS.gene08971:CDS | 45.0% | |
| TCTTACCAAGAAGGTGGTGA+TGG | - | chr4.4:14849468-14849487 | MS.gene08971:CDS | 45.0% | |
| TGCAACTTGAGGATGCAGAT+AGG | + | chr4.4:14849098-14849117 | None:intergenic | 45.0% | |
| ! | AGAGTACCAACGAGTAAGAG+TGG | + | chr4.4:14849235-14849254 | None:intergenic | 45.0% |
| TCGTTGGTACTCTGCACACA+TGG | - | chr4.4:14849242-14849261 | MS.gene08971:CDS | 50.0% | |
| TTGGTACTCTGCACACATGG+TGG | - | chr4.4:14849245-14849264 | MS.gene08971:CDS | 50.0% | |
| CTCCATGCATTCACCATCGC+CGG | - | chr4.4:14849177-14849196 | MS.gene08971:CDS | 55.0% | |
| CGGCGATGGTGAATGCATGG+AGG | + | chr4.4:14849179-14849198 | None:intergenic | 60.0% | |
| CTCCGGCGATGGTGAATGCA+TGG | + | chr4.4:14849182-14849201 | None:intergenic | 60.0% | |
| TGCAGAGGCGGAAACAGCTC+CGG | + | chr4.4:14849199-14849218 | None:intergenic | 60.0% | |
| GGCGGAAACAGCTCCGGCGA+TGG | + | chr4.4:14849193-14849212 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 14849036 | 14849560 | 14849036 | ID=MS.gene08971 |
| chr4.4 | mRNA | 14849036 | 14849560 | 14849036 | ID=MS.gene08971.t1;Parent=MS.gene08971 |
| chr4.4 | exon | 14849036 | 14849560 | 14849036 | ID=MS.gene08971.t1.exon1;Parent=MS.gene08971.t1 |
| chr4.4 | CDS | 14849036 | 14849560 | 14849036 | ID=cds.MS.gene08971.t1;Parent=MS.gene08971.t1 |
| Gene Sequence |
| Protein sequence |
