Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09732.t1 | GAU29296.1 | 89.2 | 93 | 10 | 0 | 1 | 93 | 332 | 424 | 1.20E-39 | 172.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09732.t1 | Q8W5B1 | 71.8 | 85 | 24 | 0 | 1 | 85 | 277 | 361 | 6.7e-31 | 134.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09732.t1 | A0A2Z6MA04 | 89.2 | 93 | 10 | 0 | 1 | 93 | 332 | 424 | 8.7e-40 | 172.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene09732 | 0.826078 | 3.31E-54 | -1.69E-46 |
| MS.gene049159 | MS.gene09732 | 0.81727 | 3.60E-52 | -1.69E-46 |
| MS.gene049501 | MS.gene09732 | 0.806788 | 6.94E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene09732 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene09732 | 0.824474 | 7.91E-54 | -1.69E-46 |
| MS.gene051247 | MS.gene09732 | 0.821008 | 5.07E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene09732 | 0.853113 | 2.96E-61 | -1.69E-46 |
| MS.gene051975 | MS.gene09732 | 0.812145 | 4.91E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene09732 | 0.815536 | 8.79E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene09732 | 0.808356 | 3.22E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene09732 | 0.816528 | 5.28E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene09732 | 0.821682 | 3.55E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene09732 | 0.830203 | 3.35E-55 | -1.69E-46 |
| MS.gene054775 | MS.gene09732 | 0.811175 | 7.99E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene09732 | 0.822119 | 2.81E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene09732 | 0.817394 | 3.37E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene09732 | 0.817955 | 2.52E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene09732 | 0.804414 | 2.19E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene09732 | 0.820706 | 5.95E-53 | -1.69E-46 |
| MS.gene058783 | MS.gene09732 | 0.810004 | 1.43E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene09732 | 0.804435 | 2.16E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene09732 | 0.814053 | 1.87E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene09732 | 0.826977 | 2.02E-54 | -1.69E-46 |
| MS.gene060429 | MS.gene09732 | 0.808553 | 2.93E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene09732 | 0.8061 | 9.69E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCATCCCGAACTTTATTT+TGG | 0.206594 | 5.4:-7106208 | MS.gene09732:CDS |
| AGATTACCCTTCTATCTTCC+AGG | 0.369139 | 5.4:+7107056 | None:intergenic |
| AAAAGCAGCTCATGCATCTC+TGG | 0.388906 | 5.4:-7106458 | MS.gene09732:CDS |
| ATGAGTACCAAAATAAAGTT+CGG | 0.391721 | 5.4:+7106201 | None:intergenic |
| TTGCTAACCTGGAAGATAGA+AGG | 0.399917 | 5.4:-7107063 | MS.gene09732:CDS |
| TGAGTACCAAAATAAAGTTC+GGG | 0.440705 | 5.4:+7106202 | None:intergenic |
| AGAAATTAGAGAACTATTAG+TGG | 0.442088 | 5.4:+7106164 | None:intergenic |
| AACACTTGGTTCCAGTGCCA+TGG | 0.479406 | 5.4:+7107124 | None:intergenic |
| GGCTGATGGTCATGTTCCGT+GGG | 0.496542 | 5.4:-7106270 | MS.gene09732:CDS |
| TGATGATGGAGTTGCTAACC+TGG | 0.518682 | 5.4:-7107074 | MS.gene09732:CDS |
| TGGCTGATGGTCATGTTCCG+TGG | 0.518749 | 5.4:-7106271 | MS.gene09732:CDS |
| ACACTTGGTTCCAGTGCCAT+GGG | 0.520060 | 5.4:+7107125 | None:intergenic |
| GTTAATGAAGCCCATGGCAC+TGG | 0.543354 | 5.4:-7107135 | None:intergenic |
| TGCTAACCTGGAAGATAGAA+GGG | 0.563120 | 5.4:-7107062 | MS.gene09732:intron |
| AGTGAAGATGAAGTTGATGA+TGG | 0.571990 | 5.4:-7107088 | MS.gene09732:CDS |
| GAGAACTATTAGTGGGCGAA+AGG | 0.590076 | 5.4:+7106172 | None:intergenic |
| TGTGGTAGGGTACTGGCTGA+TGG | 0.593400 | 5.4:-7106284 | MS.gene09732:intron |
| GAAATTAGAGAACTATTAGT+GGG | 0.597602 | 5.4:+7106165 | None:intergenic |
| TCATGTTCCGTGGGCTTGTG+AGG | 0.609215 | 5.4:-7106261 | MS.gene09732:CDS |
| CAACTTCATCTTCACTATCA+CGG | 0.618030 | 5.4:+7107095 | None:intergenic |
| TATCACGGTCTGATAACACT+TGG | 0.622180 | 5.4:+7107110 | None:intergenic |
| GAAAATGCCTCACAAGCCCA+CGG | 0.656006 | 5.4:+7106254 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAACATGTTTTTAAACACTA+TGG | + | chr5.4:7106423-7106442 | None:intergenic | 20.0% |
| ! | AACAACCTTGAATAATAGAT+AGG | - | chr5.4:7106452-7106471 | MS.gene09732:CDS | 25.0% |
| ! | AACATGATCACATATAGTAT+AGG | - | chr5.4:7106608-7106627 | MS.gene09732:intron | 25.0% |
| ! | ACATGATCACATATAGTATA+GGG | - | chr5.4:7106609-7106628 | MS.gene09732:intron | 25.0% |
| ! | ATCAACAATAATCAAACACA+TGG | + | chr5.4:7106972-7106991 | None:intergenic | 25.0% |
| ! | ATGAGTACCAAAATAAAGTT+CGG | + | chr5.4:7107096-7107115 | None:intergenic | 25.0% |
| ! | CAATACCTATCTATTATTCA+AGG | + | chr5.4:7106460-7106479 | None:intergenic | 25.0% |
| !!! | AGATTTTAGATTGTTACTTC+TGG | - | chr5.4:7106318-7106337 | MS.gene09732:intron | 25.0% |
| !!! | ATCTTGTGTTTTTTTAACTG+TGG | - | chr5.4:7106992-7107011 | MS.gene09732:intron | 25.0% |
| !!! | ATTTTTGTTTCTTTTCCCAA+CGG | - | chr5.4:7106914-7106933 | MS.gene09732:intron | 25.0% |
| !!! | GATTTTAGATTGTTACTTCT+GGG | - | chr5.4:7106319-7106338 | MS.gene09732:intron | 25.0% |
| AGAAAAGGATCAAAATGAAC+GGG | + | chr5.4:7106689-7106708 | None:intergenic | 30.0% | |
| AGGAATTACTAGAACAGAAA+AGG | + | chr5.4:7106704-7106723 | None:intergenic | 30.0% | |
| CAGAAAAGGATCAAAATGAA+CGG | + | chr5.4:7106690-7106709 | None:intergenic | 30.0% | |
| TGAGTACCAAAATAAAGTTC+GGG | + | chr5.4:7107095-7107114 | None:intergenic | 30.0% | |
| ! | ACTCTTTTATGAGGAAACAA+AGG | - | chr5.4:7106860-7106879 | MS.gene09732:intron | 30.0% |
| ! | CATGTTTTGTAGAATGAACA+TGG | - | chr5.4:7106342-7106361 | MS.gene09732:intron | 30.0% |
| !! | ATTCATCCCGAACTTTATTT+TGG | - | chr5.4:7107086-7107105 | MS.gene09732:CDS | 30.0% |
| !!! | ACCTTTTTTACTTTTGCATC+AGG | - | chr5.4:7106565-7106584 | MS.gene09732:intron | 30.0% |
| !!! | CCTTTTTTACTTTTGCATCA+GGG | - | chr5.4:7106566-7106585 | MS.gene09732:intron | 30.0% |
| !!! | GTGTTTTTTTAACTGTGGTA+GGG | - | chr5.4:7106997-7107016 | MS.gene09732:intron | 30.0% |
| !!! | TGTGTTTTTTTAACTGTGGT+AGG | - | chr5.4:7106996-7107015 | MS.gene09732:intron | 30.0% |
| CAACTTCATCTTCACTATCA+CGG | + | chr5.4:7106202-7106221 | None:intergenic | 35.0% | |
| CCCTGATGCAAAAGTAAAAA+AGG | + | chr5.4:7106569-7106588 | None:intergenic | 35.0% | |
| GAAAAGGATCAAAATGAACG+GGG | + | chr5.4:7106688-7106707 | None:intergenic | 35.0% | |
| GATCTTGTGCAGTTTACTAT+CGG | - | chr5.4:7106727-7106746 | MS.gene09732:intron | 35.0% | |
| GTAAACTGCACAAGATCTTA+AGG | + | chr5.4:7106724-7106743 | None:intergenic | 35.0% | |
| TAGAATGAACATGGACATGT+GGG | - | chr5.4:7106351-7106370 | MS.gene09732:intron | 35.0% | |
| !! | AGTGAAGATGAAGTTGATGA+TGG | - | chr5.4:7106206-7106225 | MS.gene09732:CDS | 35.0% |
| !!! | ATCTCTGGAACTCTTTTATG+AGG | - | chr5.4:7106851-7106870 | MS.gene09732:intron | 35.0% |
| ACTCACAGTTTACCGATAAG+TGG | - | chr5.4:7106378-7106397 | MS.gene09732:intron | 40.0% | |
| AGATTACCCTTCTATCTTCC+AGG | + | chr5.4:7106241-7106260 | None:intergenic | 40.0% | |
| ATTAATATGGTCGCCATCAG+TGG | + | chr5.4:7106889-7106908 | None:intergenic | 40.0% | |
| CTACCACTCAAACTAAACAG+CGG | + | chr5.4:7106486-7106505 | None:intergenic | 40.0% | |
| CTCACAGTTTACCGATAAGT+GGG | - | chr5.4:7106379-7106398 | MS.gene09732:intron | 40.0% | |
| GTAGAATGAACATGGACATG+TGG | - | chr5.4:7106350-7106369 | MS.gene09732:intron | 40.0% | |
| TATCACGGTCTGATAACACT+TGG | + | chr5.4:7106187-7106206 | None:intergenic | 40.0% | |
| TGCTAACCTGGAAGATAGAA+GGG | - | chr5.4:7106232-7106251 | MS.gene09732:CDS | 40.0% | |
| TTGCTAACCTGGAAGATAGA+AGG | - | chr5.4:7106231-7106250 | MS.gene09732:CDS | 40.0% | |
| TTTGAGTGGTAGGCACAAAT+TGG | - | chr5.4:7106494-7106513 | MS.gene09732:CDS | 40.0% | |
| ! | CAGCTCTTTTGCATAATGCT+TGG | + | chr5.4:7107065-7107084 | None:intergenic | 40.0% |
| !! | GCAGATGCTTGATGATTTTG+TGG | - | chr5.4:7106798-7106817 | MS.gene09732:intron | 40.0% |
| !! | TTTTAACTGTGGTAGGGTAC+TGG | - | chr5.4:7107003-7107022 | MS.gene09732:intron | 40.0% |
| !! | TTTTACTTTTGCATCAGGGC+TGG | - | chr5.4:7106570-7106589 | MS.gene09732:intron | 40.0% |
| !!! | CATGAGCTGCTTTTCATCTT+TGG | + | chr5.4:7106830-7106849 | None:intergenic | 40.0% |
| AAAAGCAGCTCATGCATCTC+TGG | - | chr5.4:7106836-7106855 | MS.gene09732:intron | 45.0% | |
| ACTCATTCTGCAGAAACCGT+TGG | + | chr5.4:7106933-7106952 | None:intergenic | 45.0% | |
| CTCATTCTGCAGAAACCGTT+GGG | + | chr5.4:7106932-7106951 | None:intergenic | 45.0% | |
| ! | GAGAACTATTAGTGGGCGAA+AGG | + | chr5.4:7107125-7107144 | None:intergenic | 45.0% |
| !! | CGCTGTTTAGTTTGAGTGGT+AGG | - | chr5.4:7106484-7106503 | MS.gene09732:CDS | 45.0% |
| !! | GAAACAAAGGTGTCCACTGA+TGG | - | chr5.4:7106873-7106892 | MS.gene09732:intron | 45.0% |
| !! | TGATGATGGAGTTGCTAACC+TGG | - | chr5.4:7106220-7106239 | MS.gene09732:CDS | 45.0% |
| !! | TTGCCGCTGTTTAGTTTGAG+TGG | - | chr5.4:7106480-7106499 | MS.gene09732:CDS | 45.0% |
| !! | ACAAAAATAAAATATTAATA+TGG | + | chr5.4:7106902-7106921 | None:intergenic | 5.0% |
| AACACTTGGTTCCAGTGCCA+TGG | + | chr5.4:7106173-7106192 | None:intergenic | 50.0% | |
| ACACTTGGTTCCAGTGCCAT+GGG | + | chr5.4:7106172-7106191 | None:intergenic | 50.0% | |
| CAGTTTACCGATAAGTGGGC+AGG | - | chr5.4:7106383-7106402 | MS.gene09732:intron | 50.0% | |
| GAAAATGCCTCACAAGCCCA+CGG | + | chr5.4:7107043-7107062 | None:intergenic | 50.0% | |
| TGAGCAACCTGCCCACTTAT+CGG | + | chr5.4:7106393-7106412 | None:intergenic | 50.0% | |
| GGCTGATGGTCATGTTCCGT+GGG | - | chr5.4:7107024-7107043 | MS.gene09732:intron | 55.0% | |
| TCATGTTCCGTGGGCTTGTG+AGG | - | chr5.4:7107033-7107052 | MS.gene09732:intron | 55.0% | |
| TGGCTGATGGTCATGTTCCG+TGG | - | chr5.4:7107023-7107042 | MS.gene09732:intron | 55.0% | |
| !! | TGTGGTAGGGTACTGGCTGA+TGG | - | chr5.4:7107010-7107029 | MS.gene09732:intron | 55.0% |
| AGTGGGCAGGTTGCTCAAGC+TGG | - | chr5.4:7106396-7106415 | MS.gene09732:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 7106169 | 7107147 | 7106169 | ID=MS.gene09732 |
| chr5.4 | mRNA | 7106169 | 7107147 | 7106169 | ID=MS.gene09732.t1;Parent=MS.gene09732 |
| chr5.4 | exon | 7107063 | 7107147 | 7107063 | ID=MS.gene09732.t1.exon1;Parent=MS.gene09732.t1 |
| chr5.4 | CDS | 7107063 | 7107147 | 7107063 | ID=cds.MS.gene09732.t1;Parent=MS.gene09732.t1 |
| chr5.4 | exon | 7106435 | 7106514 | 7106435 | ID=MS.gene09732.t1.exon2;Parent=MS.gene09732.t1 |
| chr5.4 | CDS | 7106435 | 7106514 | 7106435 | ID=cds.MS.gene09732.t1;Parent=MS.gene09732.t1 |
| chr5.4 | exon | 7106169 | 7106298 | 7106169 | ID=MS.gene09732.t1.exon3;Parent=MS.gene09732.t1 |
| chr5.4 | CDS | 7106169 | 7106298 | 7106169 | ID=cds.MS.gene09732.t1;Parent=MS.gene09732.t1 |
| Gene Sequence |
| Protein sequence |
