Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09761.t1 | XP_003611335.1 | 99.3 | 139 | 1 | 0 | 1 | 139 | 4 | 142 | 8.50E-71 | 276.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09761.t1 | Q9XHS0 | 74.1 | 139 | 35 | 1 | 1 | 138 | 4 | 142 | 4.4e-52 | 205.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09761.t1 | G7JXD6 | 99.3 | 139 | 1 | 0 | 1 | 139 | 4 | 142 | 6.1e-71 | 276.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene09761 | 0.823279 | 1.51E-53 | -1.69E-46 |
| MS.gene050134 | MS.gene09761 | 0.864127 | 1.52E-64 | -1.69E-46 |
| MS.gene050791 | MS.gene09761 | 0.841091 | 5.86E-58 | -1.69E-46 |
| MS.gene050912 | MS.gene09761 | 0.833773 | 4.40E-56 | -1.69E-46 |
| MS.gene051984 | MS.gene09761 | 0.843587 | 1.28E-58 | -1.69E-46 |
| MS.gene052664 | MS.gene09761 | 0.83189 | 1.29E-55 | -1.69E-46 |
| MS.gene053224 | MS.gene09761 | 0.888595 | 5.14E-73 | -1.69E-46 |
| MS.gene057272 | MS.gene09761 | 0.813848 | 2.08E-51 | -1.69E-46 |
| MS.gene057641 | MS.gene09761 | 0.822272 | 2.59E-53 | -1.69E-46 |
| MS.gene057643 | MS.gene09761 | 0.864146 | 1.50E-64 | -1.69E-46 |
| MS.gene060483 | MS.gene09761 | 0.833383 | 5.51E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACCAATTTGACATAATC+AGG | 0.268051 | 5.3:-6051533 | None:intergenic |
| GTTGTTGCTGCTGCAATTCC+AGG | 0.269519 | 5.3:+6051362 | MS.gene09761:CDS |
| GATGCTCTTGTGTGGTTGTC+AGG | 0.354575 | 5.3:+6051852 | MS.gene09761:CDS |
| ATGCTGCAAAACAACATTGT+AGG | 0.363171 | 5.3:-6051988 | None:intergenic |
| CATAAGCACGAGACTTTCTA+AGG | 0.363450 | 5.3:-6051421 | None:intergenic |
| CCAACCTGATTATGTCAAAT+TGG | 0.373462 | 5.3:+6051529 | MS.gene09761:CDS |
| CTTAGAAAGTCTCGTGCTTA+TGG | 0.383982 | 5.3:+6051422 | MS.gene09761:CDS |
| ATCAGGTTGGTCACAATCTT+CGG | 0.412966 | 5.3:-6051516 | None:intergenic |
| ATGATGTCCATTGGCTCGCC+TGG | 0.437864 | 5.3:-6051380 | None:intergenic |
| TCTCCAAGGGTCTTTGCACT+TGG | 0.459795 | 5.3:-6051596 | None:intergenic |
| GGAATTGCAGCAGCAACAAC+AGG | 0.463311 | 5.3:-6051359 | None:intergenic |
| GGTAAAGCTAGGAAGGTTAC+CGG | 0.476827 | 5.3:+6051830 | MS.gene09761:CDS |
| AACATTGTGCTCTGCGCAGA+GGG | 0.503587 | 5.3:-6051558 | None:intergenic |
| GACAACCACACAAGAGCATC+CGG | 0.522597 | 5.3:-6051849 | None:intergenic |
| TTACGCCAGCCCACTCTCCA+AGG | 0.529322 | 5.3:-6051610 | None:intergenic |
| CCAATTTGACATAATCAGGT+TGG | 0.530247 | 5.3:-6051529 | None:intergenic |
| TGCAAAGACCCTTGGAGAGT+GGG | 0.530356 | 5.3:+6051601 | MS.gene09761:CDS |
| TTTGTTATGCAGTGAAGAAG+AGG | 0.537025 | 5.3:+6051322 | None:intergenic |
| GTTCCAAGTGCAAAGACCCT+TGG | 0.537039 | 5.3:+6051593 | MS.gene09761:CDS |
| AAGACCCTTGGAGAGTGGGC+TGG | 0.538691 | 5.3:+6051605 | MS.gene09761:CDS |
| TTGATTCAGAAGGTAAAGCT+AGG | 0.543764 | 5.3:+6051819 | MS.gene09761:CDS |
| TTCAGAAGGTAAAGCTAGGA+AGG | 0.557714 | 5.3:+6051823 | MS.gene09761:CDS |
| TGCAATTCCAGGCGAGCCAA+TGG | 0.568250 | 5.3:+6051373 | MS.gene09761:CDS |
| GTGCAAAGACCCTTGGAGAG+TGG | 0.569975 | 5.3:+6051600 | MS.gene09761:CDS |
| ATGCTCTTGTGTGGTTGTCA+GGG | 0.586706 | 5.3:+6051853 | MS.gene09761:CDS |
| AAAGCAGTCATGATGTCCAT+TGG | 0.589394 | 5.3:-6051389 | None:intergenic |
| CTTGCACGAGGTCTTCATGA+AGG | 0.605005 | 5.3:+6051449 | MS.gene09761:CDS |
| AGAAAGTCTCGTGCTTATGG+TGG | 0.620799 | 5.3:+6051425 | MS.gene09761:CDS |
| TTGTGCAAGATTGATTCAGA+AGG | 0.623879 | 5.3:+6051809 | MS.gene09761:CDS |
| TACGCCAGCCCACTCTCCAA+GGG | 0.624257 | 5.3:-6051609 | None:intergenic |
| GCTTATGGTGGTCTTGCACG+AGG | 0.624672 | 5.3:+6051437 | MS.gene09761:CDS |
| TAACATTGTGCTCTGCGCAG+AGG | 0.632440 | 5.3:-6051559 | None:intergenic |
| GGTTACCGGATGCTCTTGTG+TGG | 0.638821 | 5.3:+6051844 | MS.gene09761:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGTTCACAATATAAATT+TGG | - | chr5.3:6051699-6051718 | None:intergenic | 15.0% |
| !! | AAAGTTCACAATATAAATTT+GGG | - | chr5.3:6051698-6051717 | None:intergenic | 15.0% |
| !! | ACAATATAAATTTGGGAATT+GGG | - | chr5.3:6051691-6051710 | None:intergenic | 20.0% |
| ! | AAAACTAGGTTAAGAATCAA+GGG | - | chr5.3:6051639-6051658 | None:intergenic | 25.0% |
| ! | ATCCTGCAAACAAAAAAAAA+AGG | - | chr5.3:6051949-6051968 | None:intergenic | 25.0% |
| ! | CACAATATAAATTTGGGAAT+TGG | - | chr5.3:6051692-6051711 | None:intergenic | 25.0% |
| ! | TAAAACTAGGTTAAGAATCA+AGG | - | chr5.3:6051640-6051659 | None:intergenic | 25.0% |
| ! | TCCTGCAAACAAAAAAAAAA+GGG | - | chr5.3:6051948-6051967 | None:intergenic | 25.0% |
| ! | TTAGCATGAAAAAAGACAAT+AGG | - | chr5.3:6051907-6051926 | None:intergenic | 25.0% |
| !!! | TTTTTTGTTTGCAGGATTTT+GGG | + | chr5.3:6051952-6051971 | MS.gene09761:intron | 25.0% |
| !!! | TTTTTTTGTTTGCAGGATTT+TGG | + | chr5.3:6051951-6051970 | MS.gene09761:intron | 25.0% |
| ACAAGGAATTGGATAAAACT+AGG | - | chr5.3:6051653-6051672 | None:intergenic | 30.0% | |
| TTCACCAATTTGACATAATC+AGG | - | chr5.3:6051536-6051555 | None:intergenic | 30.0% | |
| !! | TTTTTGTTTGCAGGATTTTG+GGG | + | chr5.3:6051953-6051972 | MS.gene09761:intron | 30.0% |
| !!! | TCCCTTTTTTTTTTGTTTGC+AGG | + | chr5.3:6051944-6051963 | MS.gene09761:intron | 30.0% |
| ATCAATCTTGCACAACTACA+TGG | - | chr5.3:6051804-6051823 | None:intergenic | 35.0% | |
| ATGCTGCAAAACAACATTGT+AGG | - | chr5.3:6051991-6052010 | None:intergenic | 35.0% | |
| CCAACCTGATTATGTCAAAT+TGG | + | chr5.3:6051529-6051548 | MS.gene09761:CDS | 35.0% | |
| CCAATTTGACATAATCAGGT+TGG | - | chr5.3:6051532-6051551 | None:intergenic | 35.0% | |
| TTGATTCAGAAGGTAAAGCT+AGG | + | chr5.3:6051819-6051838 | MS.gene09761:CDS | 35.0% | |
| ! | TTGTGCAAGATTGATTCAGA+AGG | + | chr5.3:6051809-6051828 | MS.gene09761:CDS | 35.0% |
| AAAGCAGTCATGATGTCCAT+TGG | - | chr5.3:6051392-6051411 | None:intergenic | 40.0% | |
| AATGGATGTGCACAAGGAAT+TGG | - | chr5.3:6051664-6051683 | None:intergenic | 40.0% | |
| ATTTGGGAATTGGGAGCAAA+TGG | - | chr5.3:6051682-6051701 | None:intergenic | 40.0% | |
| CATAAGCACGAGACTTTCTA+AGG | - | chr5.3:6051424-6051443 | None:intergenic | 40.0% | |
| CTTAGAAAGTCTCGTGCTTA+TGG | + | chr5.3:6051422-6051441 | MS.gene09761:CDS | 40.0% | |
| TTCAGAAGGTAAAGCTAGGA+AGG | + | chr5.3:6051823-6051842 | MS.gene09761:CDS | 40.0% | |
| !! | ATCAGGTTGGTCACAATCTT+CGG | - | chr5.3:6051519-6051538 | None:intergenic | 40.0% |
| AATGATGCAACAAGACTGCG+CGG | - | chr5.3:6051748-6051767 | None:intergenic | 45.0% | |
| AGAAAGTCTCGTGCTTATGG+TGG | + | chr5.3:6051425-6051444 | MS.gene09761:CDS | 45.0% | |
| ATGCTCTTGTGTGGTTGTCA+GGG | + | chr5.3:6051853-6051872 | MS.gene09761:CDS | 45.0% | |
| GGTAAAGCTAGGAAGGTTAC+CGG | + | chr5.3:6051830-6051849 | MS.gene09761:CDS | 45.0% | |
| !! | TTGTTTGCAGGATTTTGGGG+AGG | + | chr5.3:6051956-6051975 | MS.gene09761:intron | 45.0% |
| !!! | TATTTATTTTATTGATTTTT+GGG | + | chr5.3:6051769-6051788 | MS.gene09761:intron | 5.0% |
| !!! | TTATTTATTTTATTGATTTT+TGG | + | chr5.3:6051768-6051787 | MS.gene09761:intron | 5.0% |
| AACATTGTGCTCTGCGCAGA+GGG | - | chr5.3:6051561-6051580 | None:intergenic | 50.0% | |
| ATGATGCAACAAGACTGCGC+GGG | - | chr5.3:6051747-6051766 | None:intergenic | 50.0% | |
| CTTGCACGAGGTCTTCATGA+AGG | + | chr5.3:6051449-6051468 | MS.gene09761:CDS | 50.0% | |
| CTTGTGTGGTTGTCAGGGTA+TGG | + | chr5.3:6051858-6051877 | MS.gene09761:intron | 50.0% | |
| GATGCTCTTGTGTGGTTGTC+AGG | + | chr5.3:6051852-6051871 | MS.gene09761:CDS | 50.0% | |
| GGAATTGCAGCAGCAACAAC+AGG | - | chr5.3:6051362-6051381 | None:intergenic | 50.0% | |
| GGAGCAAATGGATGTGCACA+AGG | - | chr5.3:6051670-6051689 | None:intergenic | 50.0% | |
| GTTCCAAGTGCAAAGACCCT+TGG | + | chr5.3:6051593-6051612 | MS.gene09761:CDS | 50.0% | |
| GTTGTTGCTGCTGCAATTCC+AGG | + | chr5.3:6051362-6051381 | MS.gene09761:CDS | 50.0% | |
| TAACATTGTGCTCTGCGCAG+AGG | - | chr5.3:6051562-6051581 | None:intergenic | 50.0% | |
| TCTCCAAGGGTCTTTGCACT+TGG | - | chr5.3:6051599-6051618 | None:intergenic | 50.0% | |
| TGCAAAGACCCTTGGAGAGT+GGG | + | chr5.3:6051601-6051620 | MS.gene09761:CDS | 50.0% | |
| !! | GACAACCACACAAGAGCATC+CGG | - | chr5.3:6051852-6051871 | None:intergenic | 50.0% |
| GGTTACCGGATGCTCTTGTG+TGG | + | chr5.3:6051844-6051863 | MS.gene09761:CDS | 55.0% | |
| GTGCAAAGACCCTTGGAGAG+TGG | + | chr5.3:6051600-6051619 | MS.gene09761:CDS | 55.0% | |
| TGCAATTCCAGGCGAGCCAA+TGG | + | chr5.3:6051373-6051392 | MS.gene09761:CDS | 55.0% | |
| ! | GCTTATGGTGGTCTTGCACG+AGG | + | chr5.3:6051437-6051456 | MS.gene09761:CDS | 55.0% |
| !! | ATGATGTCCATTGGCTCGCC+TGG | - | chr5.3:6051383-6051402 | None:intergenic | 55.0% |
| AAGACCCTTGGAGAGTGGGC+TGG | + | chr5.3:6051605-6051624 | MS.gene09761:CDS | 60.0% | |
| TACGCCAGCCCACTCTCCAA+GGG | - | chr5.3:6051612-6051631 | None:intergenic | 60.0% | |
| TTACGCCAGCCCACTCTCCA+AGG | - | chr5.3:6051613-6051632 | None:intergenic | 60.0% | |
| GCAACAAGACTGCGCGGGCA+TGG | - | chr5.3:6051742-6051761 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6051334 | 6052025 | 6051334 | ID=MS.gene09761 |
| chr5.3 | mRNA | 6051334 | 6052025 | 6051334 | ID=MS.gene09761.t1;Parent=MS.gene09761 |
| chr5.3 | exon | 6051334 | 6051628 | 6051334 | ID=MS.gene09761.t1.exon1;Parent=MS.gene09761.t1 |
| chr5.3 | CDS | 6051334 | 6051628 | 6051334 | ID=cds.MS.gene09761.t1;Parent=MS.gene09761.t1 |
| chr5.3 | exon | 6051809 | 6051874 | 6051809 | ID=MS.gene09761.t1.exon2;Parent=MS.gene09761.t1 |
| chr5.3 | CDS | 6051809 | 6051874 | 6051809 | ID=cds.MS.gene09761.t1;Parent=MS.gene09761.t1 |
| chr5.3 | exon | 6051966 | 6052025 | 6051966 | ID=MS.gene09761.t1.exon3;Parent=MS.gene09761.t1 |
| chr5.3 | CDS | 6051966 | 6052025 | 6051966 | ID=cds.MS.gene09761.t1;Parent=MS.gene09761.t1 |
| Gene Sequence |
| Protein sequence |
