AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20030.t1 MTR_7g100590 97.877 471 10 0 1 471 1 471 0.0 963
MS.gene20030.t1 MTR_2g093060 53.811 433 142 14 1 408 1 400 2.46e-119 357
MS.gene20030.t1 MTR_2g093060 53.811 433 141 15 1 408 1 399 6.06e-118 354
MS.gene20030.t1 MTR_4g061200 54.273 433 135 13 1 408 1 395 2.32e-116 350
MS.gene20030.t1 MTR_4g061200 55.721 402 121 10 1 381 1 366 5.44e-114 341
MS.gene20030.t1 MTR_2g093060 50.808 433 140 15 1 408 1 385 1.13e-104 319
MS.gene20030.t1 MTR_4g061200 51.270 433 133 14 1 408 1 380 7.34e-102 312
MS.gene20030.t1 MTR_4g061200 52.488 402 119 11 1 381 1 351 3.37e-99 303
MS.gene20030.t1 MTR_5g016810 83.770 191 31 0 124 314 137 327 3.81e-97 302
MS.gene20030.t1 MTR_4g094868 83.420 193 30 1 124 314 161 353 3.08e-96 300
MS.gene20030.t1 MTR_4g094868 83.333 192 31 1 124 314 161 352 6.36e-95 296
MS.gene20030.t1 MTR_5g016810 80.711 197 32 1 124 314 137 333 3.41e-94 294
MS.gene20030.t1 MTR_7g061190 96.503 143 5 0 161 303 3 145 1.96e-85 259
MS.gene20030.t1 MTR_4g094868 80.380 158 30 1 158 314 39 196 1.13e-77 247
MS.gene20030.t1 MTR_1g049140 43.161 329 141 13 1 315 3 299 1.62e-54 187
MS.gene20030.t1 MTR_1g049140 42.553 329 143 12 1 315 3 299 6.67e-54 184
MS.gene20030.t1 MTR_4g127930 49.744 195 86 4 110 293 134 327 1.48e-51 182
MS.gene20030.t1 MTR_1g049140 50.195 257 94 13 1 242 3 240 4.78e-51 174
MS.gene20030.t1 MTR_4g130270 48.168 191 88 3 113 292 24 214 1.56e-50 176
MS.gene20030.t1 MTR_7g009410 48.515 202 87 4 136 323 65 263 1.77e-50 176
MS.gene20030.t1 MTR_7g080460 41.045 268 119 7 82 330 209 456 2.87e-49 179
MS.gene20030.t1 MTR_4g065370 52.071 169 70 3 136 293 163 331 4.25e-49 176
MS.gene20030.t1 MTR_4g065370 44.156 77 41 1 126 200 255 331 7.46e-12 67.8
MS.gene20030.t1 MTR_4g065370 42.857 91 43 2 215 296 150 240 9.80e-11 64.3
MS.gene20030.t1 MTR_2g098180 51.479 169 71 3 136 293 167 335 8.29e-49 175
MS.gene20030.t1 MTR_2g098180 44.156 77 41 1 126 200 259 335 1.37e-11 67.0
MS.gene20030.t1 MTR_5g015070 51.462 171 72 3 136 295 249 419 8.48e-49 176
MS.gene20030.t1 MTR_8g068510 49.490 196 84 5 112 295 106 298 2.30e-48 171
MS.gene20030.t1 MTR_8g068510 36.449 107 63 3 102 203 194 300 6.57e-11 64.3
MS.gene20030.t1 MTR_8g044070 44.545 220 105 5 113 323 31 242 3.48e-48 171
MS.gene20030.t1 MTR_3g103460 49.180 183 84 4 122 295 295 477 6.86e-47 172
MS.gene20030.t1 MTR_8g020510 51.807 166 72 3 136 293 248 413 9.17e-47 171
MS.gene20030.t1 MTR_6g011490 49.425 174 74 3 140 302 65 235 1.57e-46 165
MS.gene20030.t1 MTR_1g017400 50.595 168 75 3 136 295 312 479 3.83e-46 170
MS.gene20030.t1 MTR_4g097520 50.595 168 75 3 136 295 310 477 7.27e-46 169
MS.gene20030.t1 MTR_4g007770 48.598 214 88 6 130 321 30 243 1.17e-44 159
MS.gene20030.t1 MTR_8g044040 47.236 199 93 4 119 307 37 233 1.87e-44 160
MS.gene20030.t1 MTR_3g090510 80.952 105 20 0 167 271 163 267 7.54e-43 156
MS.gene20030.t1 MTR_8g468920 49.215 191 88 3 111 292 43 233 5.42e-41 151
MS.gene20030.t1 MTR_2g460730 46.465 198 96 4 116 303 25 222 3.12e-40 148
MS.gene20030.t1 MTR_5g031880 49.198 187 87 3 117 295 205 391 6.27e-40 150
MS.gene20030.t1 MTR_7g060560 79.048 105 21 1 167 271 156 259 8.03e-40 148
MS.gene20030.t1 MTR_7g091390 45.985 137 60 3 136 262 57 189 1.81e-21 96.7
MS.gene20030.t1 MTR_7g055620 93.182 44 3 0 213 256 6 49 4.98e-21 95.1
MS.gene20030.t1 MTR_1g037030 77.083 48 11 0 205 252 118 165 2.67e-18 84.0
MS.gene20030.t1 MTR_7g091390 47.573 103 47 2 163 262 3 101 6.39e-15 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20030.t1 AT2G28550 53.211 436 153 15 1 408 1 413 1.67e-108 330
MS.gene20030.t1 AT4G36920 66.406 256 76 3 117 369 110 358 1.62e-96 298
MS.gene20030.t1 AT4G36920 66.406 256 76 3 117 369 110 358 1.62e-96 298
MS.gene20030.t1 AT2G28550 50.229 436 151 16 1 408 1 398 4.27e-94 292
MS.gene20030.t1 AT5G67180 78.947 190 40 0 125 314 82 271 2.07e-90 277
MS.gene20030.t1 AT5G67180 78.947 190 40 0 125 314 82 271 3.39e-90 279
MS.gene20030.t1 AT5G67180 84.795 171 26 0 125 295 82 252 2.06e-88 271
MS.gene20030.t1 AT2G28550 57.827 313 99 8 1 289 1 304 4.10e-86 268
MS.gene20030.t1 AT2G28550 59.928 277 79 7 1 254 1 268 7.17e-76 239
MS.gene20030.t1 AT2G28550 59.928 277 79 7 1 254 1 268 7.17e-76 239
MS.gene20030.t1 AT5G67180 77.059 170 36 2 125 293 82 249 1.11e-73 233
MS.gene20030.t1 AT5G60120 38.370 503 218 18 1 455 1 459 1.61e-70 232
MS.gene20030.t1 AT5G60120 38.632 497 218 18 1 455 1 452 3.22e-70 231
MS.gene20030.t1 AT5G60120 44.110 365 138 12 1 330 1 334 6.11e-67 220
MS.gene20030.t1 AT5G60120 44.110 365 138 12 1 330 1 334 6.11e-67 220
MS.gene20030.t1 AT5G60120 44.110 365 138 12 1 330 1 334 6.11e-67 220
MS.gene20030.t1 AT5G60120 37.333 525 215 20 1 455 1 481 9.19e-67 223
MS.gene20030.t1 AT2G28550 52.713 258 88 9 156 408 4 232 8.17e-64 208
MS.gene20030.t1 AT1G72570 48.958 192 85 4 115 293 197 388 2.12e-51 179
MS.gene20030.t1 AT4G37750 43.460 237 107 4 114 323 258 494 4.89e-51 182
MS.gene20030.t1 AT5G57390 48.469 196 89 4 109 293 56 250 1.21e-50 178
MS.gene20030.t1 AT1G51190 44.206 233 103 6 72 293 139 355 2.08e-50 180
MS.gene20030.t1 AT5G57390 48.469 196 89 4 109 293 174 368 4.75e-50 179
MS.gene20030.t1 AT5G17430 48.438 192 87 4 112 292 122 312 1.19e-49 177
MS.gene20030.t1 AT5G17430 48.438 192 87 4 112 292 184 374 2.05e-49 178
MS.gene20030.t1 AT2G39250 39.701 335 123 11 1 326 1 265 3.08e-49 171
MS.gene20030.t1 AT3G20840 50.888 169 72 3 136 293 178 346 7.28e-49 176
MS.gene20030.t1 AT3G20840 44.156 77 41 1 126 200 270 346 8.73e-12 67.8
MS.gene20030.t1 AT2G39250 46.473 241 82 6 1 233 1 202 3.62e-48 166
MS.gene20030.t1 AT3G54320 46.829 205 84 4 120 299 32 236 2.21e-44 161
MS.gene20030.t1 AT5G65510 54.386 171 67 3 136 295 170 340 2.61e-44 161
MS.gene20030.t1 AT3G54320 46.602 206 85 4 120 300 32 237 2.81e-44 161
MS.gene20030.t1 AT5G65510 54.386 171 67 3 136 295 228 398 3.76e-44 162
MS.gene20030.t1 AT5G65510 54.386 171 67 3 136 295 228 398 3.76e-44 162
MS.gene20030.t1 AT3G54990 66.443 149 39 2 97 245 91 228 8.01e-44 154
MS.gene20030.t1 AT3G54990 59.016 183 50 3 97 279 91 248 1.28e-43 157
MS.gene20030.t1 AT5G10510 52.047 171 71 3 136 295 227 397 3.20e-42 157
MS.gene20030.t1 AT5G10510 52.047 171 71 3 136 295 227 397 3.20e-42 157
MS.gene20030.t1 AT5G10510 52.047 171 71 3 136 295 265 435 3.92e-42 158
MS.gene20030.t1 AT5G10510 52.047 171 71 3 136 295 265 435 3.92e-42 158
MS.gene20030.t1 AT5G10510 52.047 171 71 3 136 295 250 420 4.27e-42 157
MS.gene20030.t1 AT5G65510 53.191 141 55 3 136 265 170 310 2.48e-40 147
MS.gene20030.t1 AT1G79700 53.448 174 70 2 130 292 43 216 4.27e-40 147
MS.gene20030.t1 AT1G16060 50.000 188 83 2 116 292 35 222 3.47e-39 145
MS.gene20030.t1 AT5G10510 50.000 168 76 3 136 295 227 394 4.25e-38 146
MS.gene20030.t1 AT5G10510 45.026 191 74 4 136 295 265 455 1.86e-35 139
MS.gene20030.t1 AT1G79700 50.575 174 65 3 130 292 43 206 8.32e-33 127
MS.gene20030.t1 AT3G54320 50.725 138 67 1 163 299 33 170 1.47e-31 124
MS.gene20030.t1 AT3G54320 50.725 138 67 1 163 299 33 170 1.47e-31 124
MS.gene20030.t1 AT1G79700 54.412 136 61 1 158 292 5 140 1.12e-28 113
MS.gene20030.t1 AT1G16060 54.412 136 61 1 158 292 17 152 6.80e-28 112
MS.gene20030.t1 AT1G79700 54.264 129 58 1 165 292 6 134 1.44e-26 107
MS.gene20030.t1 AT2G41710 48.855 131 53 2 136 256 67 193 5.06e-25 107
MS.gene20030.t1 AT2G41710 48.855 131 53 2 136 256 67 193 6.92e-25 107
MS.gene20030.t1 AT2G41710 47.059 136 53 3 136 256 67 198 7.34e-23 100
MS.gene20030.t1 AT2G41710 46.281 121 51 2 146 256 112 228 3.48e-20 93.2
MS.gene20030.t1 AT3G54320 54.167 96 43 1 206 300 1 96 3.43e-19 88.2
MS.gene20030.t1 AT1G16060 55.682 88 38 1 206 292 1 88 8.42e-18 82.4
MS.gene20030.t1 AT1G16060 55.682 88 38 1 206 292 1 88 8.42e-18 82.4
MS.gene20030.t1 AT1G16060 55.682 88 38 1 206 292 1 88 8.42e-18 82.4
MS.gene20030.t1 AT1G16060 55.682 88 38 1 206 292 1 88 8.42e-18 82.4
MS.gene20030.t1 AT3G25730 38.947 95 54 2 128 222 53 143 2.55e-12 68.2
MS.gene20030.t1 AT1G68840 37.383 107 63 2 116 222 44 146 1.29e-11 66.2
MS.gene20030.t1 AT1G68840 37.383 107 63 2 116 222 44 146 1.29e-11 66.2
MS.gene20030.t1 AT2G47520 44.578 83 43 2 122 202 32 113 1.56e-11 63.2

Find 134 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGCTGCCATGCCCAATT+TGG 0.214682 7.1:-8190923 None:intergenic
ATCATTCCCGATTCGTTTGC+TGG 0.249662 7.1:-8188726 None:intergenic
CTCAATGTTCAGTCATCTTA+TGG 0.249805 7.1:+8190666 MS.gene20030:CDS
GTTTGCAAGTTCAGCGTAAC+TGG 0.251291 7.1:+8190463 MS.gene20030:CDS
AATCTGCTCCCGTTCCAAAT+TGG 0.265423 7.1:+8190909 MS.gene20030:CDS
CTCCAAAGTCCATATAATAA+TGG 0.282974 7.1:+8190941 MS.gene20030:CDS
GACGTTGGGAATCGCATATT+TGG 0.285512 7.1:+8188919 MS.gene20030:CDS
AAAGTTGACCCATTATTATA+TGG 0.312812 7.1:-8190950 None:intergenic
GCTCGTATGGGACAGTTTCT+TGG 0.324940 7.1:+8189719 MS.gene20030:CDS
GGAAGCGTTGTTGTTGAGTA+TGG 0.328168 7.1:-8188625 None:intergenic
ATCTGCTCCCGTTCCAAATT+GGG 0.340346 7.1:+8190910 MS.gene20030:CDS
CGTTGATAGTTTGAGGACTC+TGG 0.357542 7.1:+8190633 MS.gene20030:intron
ACCCATTATTATATGGACTT+TGG 0.377342 7.1:-8190943 None:intergenic
TTATGTAGGTATATATATCT+TGG 0.380122 7.1:+8189881 MS.gene20030:intron
TTCTCTGCTGCAGCATCATC+AGG 0.380972 7.1:+8190986 MS.gene20030:CDS
CTGAGCATCAACCTCCAATC+TGG 0.386362 7.1:+8190703 MS.gene20030:CDS
ACCATTACACTCTTATCAAC+AGG 0.390876 7.1:-8190495 None:intergenic
GTTACTTTCTACCGGAGAAC+TGG 0.391369 7.1:+8188897 MS.gene20030:CDS
TCCAAAGTCCATATAATAAT+GGG 0.393361 7.1:+8190942 MS.gene20030:CDS
TTCTTCACCGGCTGAGGTCG+TGG 0.400221 7.1:-8188831 None:intergenic
GATGCTGCAGCAGAGAAATT+AGG 0.402479 7.1:-8190980 None:intergenic
TAATTACTTTCCCGTGTGTA+AGG 0.413461 7.1:+8190734 MS.gene20030:CDS
CTCGTATGGGACAGTTTCTT+GGG 0.413776 7.1:+8189720 MS.gene20030:CDS
TATGTAGGTATATATATCTT+GGG 0.414146 7.1:+8189882 MS.gene20030:intron
TGCCATGCCCAATTTGGAAC+GGG 0.426003 7.1:-8190917 None:intergenic
GGGTGGAGGTATGTTATGTT+TGG 0.445569 7.1:-8191049 None:intergenic
ATTTGAACATCAGAGTTAGA+AGG 0.449742 7.1:-8190414 None:intergenic
GCCGGTGAAGAAGAGTCGTC+GGG 0.459593 7.1:+8188842 MS.gene20030:CDS
TGGACGATGGGAAGCTCGTA+TGG 0.459668 7.1:+8189706 MS.gene20030:CDS
CCTGCAGTAGAAAGAGGGTA+TGG 0.464065 7.1:-8191104 None:intergenic
TACAACAGCTTCGCCGGCGC+CGG 0.464183 7.1:-8188685 None:intergenic
GCAGAATCTTGATCTGAACT+TGG 0.465184 7.1:+8190380 MS.gene20030:CDS
CTATCCGCAGCTGTTGTTGA+AGG 0.465186 7.1:-8191013 None:intergenic
AAGAGCAATAGAGAATAGGA+TGG 0.477231 7.1:+8190886 MS.gene20030:CDS
ACTCTCCTCCGTCGTTCAGA+TGG 0.481608 7.1:+8188509 MS.gene20030:CDS
CTACCGGAGAACTGGACGTT+GGG 0.483697 7.1:+8188905 MS.gene20030:CDS
AGGAAAGAGCAATAGAGAAT+AGG 0.487980 7.1:+8190882 MS.gene20030:intron
CCATACCCTCTTTCTACTGC+AGG 0.495357 7.1:+8191104 MS.gene20030:CDS
TTTGAGGCTAGTTCTTATGA+AGG 0.495376 7.1:+8190121 MS.gene20030:CDS
ACTGAAGATTCATGCTCTTA+CGG 0.496241 7.1:+8188579 MS.gene20030:CDS
TAGTACCATCGTTATCAGCC+TGG 0.496630 7.1:-8190156 None:intergenic
TGCTCTTACGGCGATGCATA+CGG 0.502837 7.1:+8188591 MS.gene20030:CDS
TGTCCGTACAACAGCTTCGC+CGG 0.508835 7.1:-8188691 None:intergenic
GTAGAAAGAGGGTATGGGGC+TGG 0.510625 7.1:-8191098 None:intergenic
ATCGTGGTGTTACTTTCTAC+CGG 0.518398 7.1:+8188889 MS.gene20030:CDS
TAATTAGTACCACTCCAGAT+TGG 0.520137 7.1:-8190717 None:intergenic
TGTTGCTCCACGACCTCAGC+CGG 0.520275 7.1:+8188824 MS.gene20030:CDS
CGACTCTTCTTCACCGGCTG+AGG 0.520783 7.1:-8188837 None:intergenic
AACATGCACCACAGTGGAAG+TGG 0.520906 7.1:+8190441 MS.gene20030:CDS
ATTCCCAACGTCCAGTTCTC+CGG 0.521427 7.1:-8188908 None:intergenic
ATGGGGCTGGAGTTAGTCGC+TGG 0.523340 7.1:-8191085 None:intergenic
TGAAGGAGAGTTAACTTCCC+AGG 0.523464 7.1:+8190138 MS.gene20030:CDS
GTCGCTGGAGTAGGAAAATG+AGG 0.525924 7.1:-8191070 None:intergenic
GAGCTTCAACACTCCGGCGC+CGG 0.528911 7.1:+8188672 MS.gene20030:CDS
CGGTACGCCACCGTGATGAT+CGG 0.529586 7.1:-8188787 None:intergenic
AGGTTGATGCTCAGATGCTA+TGG 0.530023 7.1:-8190694 None:intergenic
TGTGGAAAACAAGTATACTT+AGG 0.532079 7.1:+8189057 MS.gene20030:CDS
CTGCTATAAAATGCAATGGA+AGG 0.535486 7.1:+8190083 MS.gene20030:CDS
CTGCAGTAGAAAGAGGGTAT+GGG 0.539113 7.1:-8191103 None:intergenic
CGATCATCACGGTGGCGTAC+CGG 0.539554 7.1:+8188788 MS.gene20030:CDS
CCCCGACGACTCTTCTTCAC+CGG 0.545486 7.1:-8188843 None:intergenic
CTGGAGTTAGTCGCTGGAGT+AGG 0.547085 7.1:-8191079 None:intergenic
GCCGGAGTGTTGAAGCTCAC+CGG 0.549298 7.1:-8188667 None:intergenic
GTTGATAGTTTGAGGACTCT+GGG 0.550553 7.1:+8190634 MS.gene20030:intron
GACAGAGTACTGGTTTCTCA+AGG 0.553049 7.1:+8189645 MS.gene20030:CDS
TCAGCTCCTGCAGTAGAAAG+AGG 0.553716 7.1:-8191110 None:intergenic
TGATTACAGAGCGTATGATA+AGG 0.555672 7.1:+8190060 MS.gene20030:intron
CTCCCGTTCCAAATTGGGCA+TGG 0.556092 7.1:+8190915 MS.gene20030:CDS
TTATGATGAAGATATAAAGC+AGG 0.557521 7.1:+8189392 MS.gene20030:CDS
GCGCCGGCGAAGCTGTTGTA+CGG 0.557964 7.1:+8188688 MS.gene20030:CDS
TACTGCGAGCTTCGTGACCT+CGG 0.565421 7.1:-8188867 None:intergenic
ATCTGAACGACGGAGGAGAG+TGG 0.568541 7.1:-8188507 None:intergenic
ATTCTGCGTAGACAGAGTAC+TGG 0.571541 7.1:+8189635 MS.gene20030:CDS
GAGTAGGAAAATGAGGGTGG+AGG 0.571882 7.1:-8191063 None:intergenic
AGCCGGTGAAGAAGAGTCGT+CGG 0.574425 7.1:+8188841 MS.gene20030:CDS
GTTCAGATGGAAGATTCATC+CGG 0.575771 7.1:+8188522 MS.gene20030:CDS
CGAGCTGCGATAAAGTTTCG+TGG 0.575785 7.1:+8189336 MS.gene20030:CDS
TGCAGTAGAAAGAGGGTATG+GGG 0.576798 7.1:-8191102 None:intergenic
TATCCGCAGCTGTTGTTGAA+GGG 0.577107 7.1:-8191012 None:intergenic
TGCTATAAAATGCAATGGAA+GGG 0.577688 7.1:+8190084 MS.gene20030:CDS
CGCCTTCTGAGAGTAGAGGA+TGG 0.578820 7.1:+8188751 MS.gene20030:CDS
GTCGACAACGGTACTATCTC+CGG 0.580552 7.1:+8188648 MS.gene20030:CDS
TTACCTTGCCGCAGCATGCG+CGG 0.580853 7.1:-8189205 None:intergenic
TCTACCGGAGAACTGGACGT+TGG 0.583316 7.1:+8188904 MS.gene20030:CDS
ATACCGCGCATGCTGCGGCA+AGG 0.583419 7.1:+8189202 MS.gene20030:CDS
GCAACAACAACAGTCTGCTC+CGG 0.583622 7.1:-8188807 None:intergenic
CAGAATCTTGATCTGAACTT+GGG 0.584312 7.1:+8190381 MS.gene20030:CDS
AAGGCTGCTATAAAATGCAA+TGG 0.584344 7.1:+8190079 MS.gene20030:CDS
TTAGTACCACTCCAGATTGG+AGG 0.585536 7.1:-8190714 None:intergenic
GACGGAGGAATCAAAGCTAC+CGG 0.588222 7.1:-8188541 None:intergenic
TTTGCAAGTTCAGCGTAACT+GGG 0.588454 7.1:+8190464 MS.gene20030:CDS
TCTGCATAAATGTGGACGAT+GGG 0.589018 7.1:+8189694 MS.gene20030:CDS
AGTACCATCGTTATCAGCCT+GGG 0.589962 7.1:-8190155 None:intergenic
AGAAGAGTCGTCGGGGACCG+AGG 0.591121 7.1:+8188850 MS.gene20030:CDS
ATCCATCCTCTACTCTCAGA+AGG 0.594152 7.1:-8188753 None:intergenic
CTGCCATGCCCAATTTGGAA+CGG 0.595613 7.1:-8190918 None:intergenic
GACGGAGGAGAGTGGAAGAG+AGG 0.597615 7.1:-8188499 None:intergenic
ATAGCGAGCTAGATGCTGCA+AGG 0.598807 7.1:+8189912 MS.gene20030:CDS
ACAGAGTACTGGTTTCTCAA+GGG 0.599902 7.1:+8189646 MS.gene20030:CDS
ATTTGATACCGCGCATGCTG+CGG 0.600999 7.1:+8189197 MS.gene20030:CDS
CAGAGTACTGGTTTCTCAAG+GGG 0.605158 7.1:+8189647 MS.gene20030:CDS
AAAGTTTCGTGGCGTCGATG+CGG 0.606313 7.1:+8189347 MS.gene20030:CDS
GGACGATGGGAAGCTCGTAT+GGG 0.611195 7.1:+8189707 MS.gene20030:CDS
CAGCTCCTGCAGTAGAAAGA+GGG 0.613397 7.1:-8191109 None:intergenic
ATTAGTATTCACCTTACACA+CGG 0.613966 7.1:-8190745 None:intergenic
TGAACATCAGAGTTAGAAGG+AGG 0.614498 7.1:-8190411 None:intergenic
GCCTGTTGATAAGAGTGTAA+TGG 0.619307 7.1:+8190494 MS.gene20030:CDS
AATTTCTCGTCCGATCATCA+CGG 0.619679 7.1:+8188777 MS.gene20030:CDS
AACAACGCTTCCGTCGACAA+CGG 0.620133 7.1:+8188636 MS.gene20030:CDS
GGAGTTACTCTGCATAAATG+TGG 0.624873 7.1:+8189686 MS.gene20030:CDS
CTCTGCATAAATGTGGACGA+TGG 0.629073 7.1:+8189693 MS.gene20030:CDS
ATCTCGCCTTCTGAGAGTAG+AGG 0.632485 7.1:+8188747 MS.gene20030:CDS
TCCGGTGAGCTTCAACACTC+CGG 0.632556 7.1:+8188666 MS.gene20030:CDS
GTAGATGAATAACTTTACAA+AGG 0.633971 7.1:+8189598 MS.gene20030:intron
TCACGAAGCTCGCAGTATCG+TGG 0.635514 7.1:+8188873 MS.gene20030:CDS
AGAGCATGAATCTTCAGTGT+CGG 0.641138 7.1:-8188574 None:intergenic
GGTTGATGCTCAGATGCTAT+GGG 0.641889 7.1:-8190693 None:intergenic
GTCGGCAGCGTTGACGACGG+AGG 0.642992 7.1:-8188556 None:intergenic
GCTGCAGCAGAGAAATTAGG+TGG 0.656992 7.1:-8190977 None:intergenic
CTGGAGTAGGAAAATGAGGG+TGG 0.661562 7.1:-8191066 None:intergenic
CATCAACCTCCAATCTGGAG+TGG 0.662314 7.1:+8190708 MS.gene20030:CDS
GGAGATAGTACCGTTGTCGA+CGG 0.663908 7.1:-8188646 None:intergenic
ATCTTCCATCTGAACGACGG+AGG 0.664445 7.1:-8188514 None:intergenic
AGTGTCGGCAGCGTTGACGA+CGG 0.674336 7.1:-8188559 None:intergenic
TTAGTATTCACCTTACACAC+GGG 0.680796 7.1:-8190744 None:intergenic
AGGGGAAGTTCTAAATACAG+AGG 0.681558 7.1:+8189665 MS.gene20030:CDS
ATTCCCTTCAACAACAGCTG+CGG 0.681754 7.1:+8191009 MS.gene20030:CDS
TGAATCTTCCATCTGAACGA+CGG 0.693422 7.1:-8188517 None:intergenic
TCGCTGGAGTAGGAAAATGA+GGG 0.701704 7.1:-8191069 None:intergenic
CCGGTGAAGAAGAGTCGTCG+GGG 0.715698 7.1:+8188843 MS.gene20030:CDS
TTCTCGTCCGATCATCACGG+TGG 0.718908 7.1:+8188780 MS.gene20030:CDS
ACTTCCCAGGCTGATAACGA+TGG 0.728400 7.1:+8190151 MS.gene20030:CDS
ATGATGAACATGCACCACAG+TGG 0.729813 7.1:+8190435 MS.gene20030:CDS
CTTGCAAACCACTTCCACTG+TGG 0.732557 7.1:-8190449 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATCAAATTAAACTAA+AGG - chr7.1:8189761-8189780 None:intergenic 10.0%
!!! TATTTAGTTCTTAATTATAT+TGG + chr7.1:8189829-8189848 MS.gene20030:intron 10.0%
!! AAAGATCAAAATCTAAAAAA+AGG - chr7.1:8189156-8189175 None:intergenic 15.0%
!! ATGATCCAAAATATTTAATT+AGG - chr7.1:8189983-8190002 None:intergenic 15.0%
!! TATAATTAAGAACTAAATAC+TGG - chr7.1:8189828-8189847 None:intergenic 15.0%
!! TGATTATAAAACCAAAAAAA+AGG - chr7.1:8190203-8190222 None:intergenic 15.0%
!!! AAATGCCTAATTAAATATTT+TGG + chr7.1:8189975-8189994 MS.gene20030:intron 15.0%
!!! TAAATATTGAATGTTTTTCA+TGG + chr7.1:8189268-8189287 MS.gene20030:intron 15.0%
!!! TCTTTGAAATTTAATTTTTC+AGG + chr7.1:8189171-8189190 MS.gene20030:intron 15.0%
!! ATTATTTGTGTTCTATTTAC+AGG + chr7.1:8190862-8190881 MS.gene20030:intron 20.0%
!! ATTTATCAATCATATATAGC+AGG + chr7.1:8189940-8189959 MS.gene20030:intron 20.0%
!! TATGTAGGTATATATATCTT+GGG + chr7.1:8189882-8189901 MS.gene20030:intron 20.0%
!! TTATGTAGGTATATATATCT+TGG + chr7.1:8189881-8189900 MS.gene20030:intron 20.0%
!!! TTGAAATTTAATTTTTCAGG+TGG + chr7.1:8189174-8189193 MS.gene20030:intron 20.0%
! AAAGTTGACCCATTATTATA+TGG - chr7.1:8190953-8190972 None:intergenic 25.0%
! AGGTTAATTGACATACTTTA+AGG - chr7.1:8190287-8190306 None:intergenic 25.0%
! AGTTCTTAATTATATTGGTC+TGG + chr7.1:8189834-8189853 MS.gene20030:intron 25.0%
! GTAGATGAATAACTTTACAA+AGG + chr7.1:8189598-8189617 MS.gene20030:intron 25.0%
! TCCAAAGTCCATATAATAAT+GGG + chr7.1:8190942-8190961 MS.gene20030:CDS 25.0%
! TTAACCTTTACTATTAAGAC+TGG + chr7.1:8190300-8190319 MS.gene20030:intron 25.0%
! TTATGATGAAGATATAAAGC+AGG + chr7.1:8189392-8189411 MS.gene20030:CDS 25.0%
!! GTTCTTAATTATATTGGTCT+GGG + chr7.1:8189835-8189854 MS.gene20030:intron 25.0%
!! GTTGTTAATTATATTGGTCT+GGG + chr7.1:8189857-8189876 MS.gene20030:intron 25.0%
!!! AAGAACTAAATACTGGTTTA+TGG - chr7.1:8189821-8189840 None:intergenic 25.0%
!!! AATCACAAATCAGTCATTTT+AGG - chr7.1:8189791-8189810 None:intergenic 25.0%
ACCCATTATTATATGGACTT+TGG - chr7.1:8190946-8190965 None:intergenic 30.0%
AGAGTGAGTGATAGTTTATA+TGG - chr7.1:8190250-8190269 None:intergenic 30.0%
ATATCTTAAGAATCTTCTGC+TGG + chr7.1:8190827-8190846 MS.gene20030:intron 30.0%
ATCACCAGTCTTAATAGTAA+AGG - chr7.1:8190307-8190326 None:intergenic 30.0%
ATTAGTATTCACCTTACACA+CGG - chr7.1:8190748-8190767 None:intergenic 30.0%
ATTTGAACATCAGAGTTAGA+AGG - chr7.1:8190417-8190436 None:intergenic 30.0%
CTCCAAAGTCCATATAATAA+TGG + chr7.1:8190941-8190960 MS.gene20030:CDS 30.0%
GGTTGTTAATTATATTGGTC+TGG + chr7.1:8189856-8189875 MS.gene20030:intron 30.0%
GTCTGGGTTGTTAATTATAT+TGG + chr7.1:8189851-8189870 MS.gene20030:intron 30.0%
TGCTATAAAATGCAATGGAA+GGG + chr7.1:8190084-8190103 MS.gene20030:CDS 30.0%
TGTGGAAAACAAGTATACTT+AGG + chr7.1:8189057-8189076 MS.gene20030:CDS 30.0%
! TACATGTTGTGTTATTGACT+TGG + chr7.1:8190025-8190044 MS.gene20030:intron 30.0%
! TTGATAAGAGTGTAATGGTA+AGG + chr7.1:8190499-8190518 MS.gene20030:intron 30.0%
!! TCTGTTTTCGTTTTTCTGTA+GGG + chr7.1:8189032-8189051 MS.gene20030:intron 30.0%
AAAAAAAAGGTGAGCCTCAA+TGG - chr7.1:8190190-8190209 None:intergenic 35.0%
AAGAGCAATAGAGAATAGGA+TGG + chr7.1:8190886-8190905 MS.gene20030:CDS 35.0%
AAGGCTGCTATAAAATGCAA+TGG + chr7.1:8190079-8190098 MS.gene20030:CDS 35.0%
AATTCATGTGAAAGAGCAGA+GGG - chr7.1:8190571-8190590 None:intergenic 35.0%
ACAGAAAAACGAAAACAGAG+TGG - chr7.1:8189031-8189050 None:intergenic 35.0%
ACCATTACACTCTTATCAAC+AGG - chr7.1:8190498-8190517 None:intergenic 35.0%
ACTGAAGATTCATGCTCTTA+CGG + chr7.1:8188579-8188598 MS.gene20030:CDS 35.0%
AGGAAAGAGCAATAGAGAAT+AGG + chr7.1:8190882-8190901 MS.gene20030:intron 35.0%
ATTTGGTTAGCATTTCGCAA+TGG + chr7.1:8188936-8188955 MS.gene20030:intron 35.0%
CAGAATCTTGATCTGAACTT+GGG + chr7.1:8190381-8190400 MS.gene20030:CDS 35.0%
CTCAATGTTCAGTCATCTTA+TGG + chr7.1:8190666-8190685 MS.gene20030:CDS 35.0%
CTGCTATAAAATGCAATGGA+AGG + chr7.1:8190083-8190102 MS.gene20030:CDS 35.0%
GTACTACTTATTGACCATTG+AGG + chr7.1:8190173-8190192 MS.gene20030:intron 35.0%
TAATTACTTTCCCGTGTGTA+AGG + chr7.1:8190734-8190753 MS.gene20030:CDS 35.0%
TAATTAGTACCACTCCAGAT+TGG - chr7.1:8190720-8190739 None:intergenic 35.0%
TGATTACAGAGCGTATGATA+AGG + chr7.1:8190060-8190079 MS.gene20030:intron 35.0%
TTAGTATTCACCTTACACAC+GGG - chr7.1:8190747-8190766 None:intergenic 35.0%
! ATATTGGTCTGGGTTTATGT+AGG + chr7.1:8189867-8189886 MS.gene20030:intron 35.0%
! GGAAGCAGTTACAAATTTTG+AGG + chr7.1:8190105-8190124 MS.gene20030:CDS 35.0%
! TTTGAGGCTAGTTCTTATGA+AGG + chr7.1:8190121-8190140 MS.gene20030:CDS 35.0%
!! CTCTGTTTTCGTTTTTCTGT+AGG + chr7.1:8189031-8189050 MS.gene20030:intron 35.0%
!! TAATCTGCGTTGATAGTTTG+AGG + chr7.1:8190626-8190645 MS.gene20030:intron 35.0%
!!! ATTGAGGCTCACCTTTTTTT+TGG + chr7.1:8190189-8190208 MS.gene20030:intron 35.0%
AATTTCTCGTCCGATCATCA+CGG + chr7.1:8188777-8188796 MS.gene20030:CDS 40.0%
AGAGCATGAATCTTCAGTGT+CGG - chr7.1:8188577-8188596 None:intergenic 40.0%
ATTCATGTGAAAGAGCAGAG+GGG - chr7.1:8190570-8190589 None:intergenic 40.0%
GAATTCATGTGAAAGAGCAG+AGG - chr7.1:8190572-8190591 None:intergenic 40.0%
GCAGAATCTTGATCTGAACT+TGG + chr7.1:8190380-8190399 MS.gene20030:CDS 40.0%
GGAGTTACTCTGCATAAATG+TGG + chr7.1:8189686-8189705 MS.gene20030:CDS 40.0%
GTTCAGATGGAAGATTCATC+CGG + chr7.1:8188522-8188541 MS.gene20030:CDS 40.0%
TCTGCATAAATGTGGACGAT+GGG + chr7.1:8189694-8189713 MS.gene20030:CDS 40.0%
TGAACATCAGAGTTAGAAGG+AGG - chr7.1:8190414-8190433 None:intergenic 40.0%
TGAATCTTCCATCTGAACGA+CGG - chr7.1:8188520-8188539 None:intergenic 40.0%
TTTGCAAGTTCAGCGTAACT+GGG + chr7.1:8190464-8190483 MS.gene20030:CDS 40.0%
! CTTTTTCCAGCAAACGAATC+GGG + chr7.1:8188720-8188739 MS.gene20030:CDS 40.0%
! GCCTGTTGATAAGAGTGTAA+TGG + chr7.1:8190494-8190513 MS.gene20030:CDS 40.0%
!! ACAGAGTACTGGTTTCTCAA+GGG + chr7.1:8189646-8189665 MS.gene20030:CDS 40.0%
!! AGGGGAAGTTCTAAATACAG+AGG + chr7.1:8189665-8189684 MS.gene20030:CDS 40.0%
!! ATCGTGGTGTTACTTTCTAC+CGG + chr7.1:8188889-8188908 MS.gene20030:CDS 40.0%
!! GCTTTTTCCAGCAAACGAAT+CGG + chr7.1:8188719-8188738 MS.gene20030:CDS 40.0%
!! GTTGATAGTTTGAGGACTCT+GGG + chr7.1:8190634-8190653 MS.gene20030:intron 40.0%
!! TCGTTTTTCTGTAGGGATTG+TGG + chr7.1:8189039-8189058 MS.gene20030:intron 40.0%
AATCTGCTCCCGTTCCAAAT+TGG + chr7.1:8190909-8190928 MS.gene20030:CDS 45.0%
AGTACCATCGTTATCAGCCT+GGG - chr7.1:8190158-8190177 None:intergenic 45.0%
ATCCATCCTCTACTCTCAGA+AGG - chr7.1:8188756-8188775 None:intergenic 45.0%
ATCTGCTCCCGTTCCAAATT+GGG + chr7.1:8190910-8190929 MS.gene20030:CDS 45.0%
ATGATGAACATGCACCACAG+TGG + chr7.1:8190435-8190454 MS.gene20030:CDS 45.0%
ATTCCCTTCAACAACAGCTG+CGG + chr7.1:8191009-8191028 MS.gene20030:CDS 45.0%
ATTCTGCGTAGACAGAGTAC+TGG + chr7.1:8189635-8189654 MS.gene20030:CDS 45.0%
CTCGTATGGGACAGTTTCTT+GGG + chr7.1:8189720-8189739 MS.gene20030:CDS 45.0%
CTCTGCATAAATGTGGACGA+TGG + chr7.1:8189693-8189712 MS.gene20030:CDS 45.0%
GACGTTGGGAATCGCATATT+TGG + chr7.1:8188919-8188938 MS.gene20030:CDS 45.0%
GATGCTGCAGCAGAGAAATT+AGG - chr7.1:8190983-8191002 None:intergenic 45.0%
GTTTGCAAGTTCAGCGTAAC+TGG + chr7.1:8190463-8190482 MS.gene20030:CDS 45.0%
TAGTACCATCGTTATCAGCC+TGG - chr7.1:8190159-8190178 None:intergenic 45.0%
TATCCGCAGCTGTTGTTGAA+GGG - chr7.1:8191015-8191034 None:intergenic 45.0%
TCGCTGGAGTAGGAAAATGA+GGG - chr7.1:8191072-8191091 None:intergenic 45.0%
TGAAGGAGAGTTAACTTCCC+AGG + chr7.1:8190138-8190157 MS.gene20030:CDS 45.0%
TTAGTACCACTCCAGATTGG+AGG - chr7.1:8190717-8190736 None:intergenic 45.0%
! ATCATTCCCGATTCGTTTGC+TGG - chr7.1:8188729-8188748 None:intergenic 45.0%
! CTGCAGTAGAAAGAGGGTAT+GGG - chr7.1:8191106-8191125 None:intergenic 45.0%
! GGGTGGAGGTATGTTATGTT+TGG - chr7.1:8191052-8191071 None:intergenic 45.0%
! GGTTGATGCTCAGATGCTAT+GGG - chr7.1:8190696-8190715 None:intergenic 45.0%
! GTTACTTTCTACCGGAGAAC+TGG + chr7.1:8188897-8188916 MS.gene20030:CDS 45.0%
! TGCAGTAGAAAGAGGGTATG+GGG - chr7.1:8191105-8191124 None:intergenic 45.0%
!! AGGTTGATGCTCAGATGCTA+TGG - chr7.1:8190697-8190716 None:intergenic 45.0%
!! CAGAGTACTGGTTTCTCAAG+GGG + chr7.1:8189647-8189666 MS.gene20030:CDS 45.0%
!! CGTTGATAGTTTGAGGACTC+TGG + chr7.1:8190633-8190652 MS.gene20030:intron 45.0%
!! GACAGAGTACTGGTTTCTCA+AGG + chr7.1:8189645-8189664 MS.gene20030:CDS 45.0%
!! GGAAGCGTTGTTGTTGAGTA+TGG - chr7.1:8188628-8188647 None:intergenic 45.0%
AACAACGCTTCCGTCGACAA+CGG + chr7.1:8188636-8188655 MS.gene20030:CDS 50.0%
AACATGCACCACAGTGGAAG+TGG + chr7.1:8190441-8190460 MS.gene20030:CDS 50.0%
ATAGCGAGCTAGATGCTGCA+AGG + chr7.1:8189912-8189931 MS.gene20030:CDS 50.0%
ATCTTCCATCTGAACGACGG+AGG - chr7.1:8188517-8188536 None:intergenic 50.0%
ATTCCCAACGTCCAGTTCTC+CGG - chr7.1:8188911-8188930 None:intergenic 50.0%
ATTTGATACCGCGCATGCTG+CGG + chr7.1:8189197-8189216 MS.gene20030:CDS 50.0%
CAGCTCCTGCAGTAGAAAGA+GGG - chr7.1:8191112-8191131 None:intergenic 50.0%
CATCAACCTCCAATCTGGAG+TGG + chr7.1:8190708-8190727 MS.gene20030:CDS 50.0%
CCATACCCTCTTTCTACTGC+AGG + chr7.1:8191104-8191123 MS.gene20030:CDS 50.0%
CCTGCAGTAGAAAGAGGGTA+TGG - chr7.1:8191107-8191126 None:intergenic 50.0%
CGAGCTGCGATAAAGTTTCG+TGG + chr7.1:8189336-8189355 MS.gene20030:CDS 50.0%
CTATCCGCAGCTGTTGTTGA+AGG - chr7.1:8191016-8191035 None:intergenic 50.0%
CTGAGCATCAACCTCCAATC+TGG + chr7.1:8190703-8190722 MS.gene20030:CDS 50.0%
CTGCCATGCCCAATTTGGAA+CGG - chr7.1:8190921-8190940 None:intergenic 50.0%
CTGGAGTAGGAAAATGAGGG+TGG - chr7.1:8191069-8191088 None:intergenic 50.0%
CTTGCAAACCACTTCCACTG+TGG - chr7.1:8190452-8190471 None:intergenic 50.0%
GACGGAGGAATCAAAGCTAC+CGG - chr7.1:8188544-8188563 None:intergenic 50.0%
GAGTAGGAAAATGAGGGTGG+AGG - chr7.1:8191066-8191085 None:intergenic 50.0%
GCAACAACAACAGTCTGCTC+CGG - chr7.1:8188810-8188829 None:intergenic 50.0%
GCTCGTATGGGACAGTTTCT+TGG + chr7.1:8189719-8189738 MS.gene20030:CDS 50.0%
GCTGCAGCAGAGAAATTAGG+TGG - chr7.1:8190980-8190999 None:intergenic 50.0%
GGAGATAGTACCGTTGTCGA+CGG - chr7.1:8188649-8188668 None:intergenic 50.0%
GTCGACAACGGTACTATCTC+CGG + chr7.1:8188648-8188667 MS.gene20030:CDS 50.0%
GTCGCTGGAGTAGGAAAATG+AGG - chr7.1:8191073-8191092 None:intergenic 50.0%
TCAGCTCCTGCAGTAGAAAG+AGG - chr7.1:8191113-8191132 None:intergenic 50.0%
TGCTCTTACGGCGATGCATA+CGG + chr7.1:8188591-8188610 MS.gene20030:CDS 50.0%
TTCTCTGCTGCAGCATCATC+AGG + chr7.1:8190986-8191005 MS.gene20030:CDS 50.0%
! ACTTCCCAGGCTGATAACGA+TGG + chr7.1:8190151-8190170 MS.gene20030:CDS 50.0%
! ATCTCGCCTTCTGAGAGTAG+AGG + chr7.1:8188747-8188766 MS.gene20030:CDS 50.0%
! TGCCATGCCCAATTTGGAAC+GGG - chr7.1:8190920-8190939 None:intergenic 50.0%
!! AAAGTTTCGTGGCGTCGATG+CGG + chr7.1:8189347-8189366 MS.gene20030:CDS 50.0%
ACTCTCCTCCGTCGTTCAGA+TGG + chr7.1:8188509-8188528 MS.gene20030:CDS 55.0%
AGCCGGTGAAGAAGAGTCGT+CGG + chr7.1:8188841-8188860 MS.gene20030:CDS 55.0%
ATCTGAACGACGGAGGAGAG+TGG - chr7.1:8188510-8188529 None:intergenic 55.0%
CTACCGGAGAACTGGACGTT+GGG + chr7.1:8188905-8188924 MS.gene20030:CDS 55.0%
CTCCCGTTCCAAATTGGGCA+TGG + chr7.1:8190915-8190934 MS.gene20030:CDS 55.0%
CTGGAGTTAGTCGCTGGAGT+AGG - chr7.1:8191082-8191101 None:intergenic 55.0%
GGACGATGGGAAGCTCGTAT+GGG + chr7.1:8189707-8189726 MS.gene20030:CDS 55.0%
TACTGCGAGCTTCGTGACCT+CGG - chr7.1:8188870-8188889 None:intergenic 55.0%
TCACGAAGCTCGCAGTATCG+TGG + chr7.1:8188873-8188892 MS.gene20030:CDS 55.0%
TCCGGTGAGCTTCAACACTC+CGG + chr7.1:8188666-8188685 MS.gene20030:CDS 55.0%
TCTACCGGAGAACTGGACGT+TGG + chr7.1:8188904-8188923 MS.gene20030:CDS 55.0%
TGGACGATGGGAAGCTCGTA+TGG + chr7.1:8189706-8189725 MS.gene20030:CDS 55.0%
TGGAGCTGCCATGCCCAATT+TGG - chr7.1:8190926-8190945 None:intergenic 55.0%
TGTCCGTACAACAGCTTCGC+CGG - chr7.1:8188694-8188713 None:intergenic 55.0%
TTCTCGTCCGATCATCACGG+TGG + chr7.1:8188780-8188799 MS.gene20030:CDS 55.0%
! CGCCTTCTGAGAGTAGAGGA+TGG + chr7.1:8188751-8188770 MS.gene20030:CDS 55.0%
! GTAGAAAGAGGGTATGGGGC+TGG - chr7.1:8191101-8191120 None:intergenic 55.0%
! TGAAAGAGCAGAGGGGCATG+AGG - chr7.1:8190563-8190582 None:intergenic 55.0%
ATGGGGCTGGAGTTAGTCGC+TGG - chr7.1:8191088-8191107 None:intergenic 60.0%
CCCCGACGACTCTTCTTCAC+CGG - chr7.1:8188846-8188865 None:intergenic 60.0%
CCGGTGAAGAAGAGTCGTCG+GGG + chr7.1:8188843-8188862 MS.gene20030:CDS 60.0%
CGATCATCACGGTGGCGTAC+CGG + chr7.1:8188788-8188807 MS.gene20030:CDS 60.0%
CGGTACGCCACCGTGATGAT+CGG - chr7.1:8188790-8188809 None:intergenic 60.0%
GACGGAGGAGAGTGGAAGAG+AGG - chr7.1:8188502-8188521 None:intergenic 60.0%
GCCGGTGAAGAAGAGTCGTC+GGG + chr7.1:8188842-8188861 MS.gene20030:CDS 60.0%
TGTTGCTCCACGACCTCAGC+CGG + chr7.1:8188824-8188843 MS.gene20030:CDS 60.0%
TTACCTTGCCGCAGCATGCG+CGG - chr7.1:8189208-8189227 None:intergenic 60.0%
! CGACTCTTCTTCACCGGCTG+AGG - chr7.1:8188840-8188859 None:intergenic 60.0%
! TTCTTCACCGGCTGAGGTCG+TGG - chr7.1:8188834-8188853 None:intergenic 60.0%
!! AGTGTCGGCAGCGTTGACGA+CGG - chr7.1:8188562-8188581 None:intergenic 60.0%
!! GCCGGAGTGTTGAAGCTCAC+CGG - chr7.1:8188670-8188689 None:intergenic 60.0%
AGAAGAGTCGTCGGGGACCG+AGG + chr7.1:8188850-8188869 MS.gene20030:CDS 65.0%
ATACCGCGCATGCTGCGGCA+AGG + chr7.1:8189202-8189221 MS.gene20030:CDS 65.0%
GAGCTTCAACACTCCGGCGC+CGG + chr7.1:8188672-8188691 MS.gene20030:CDS 65.0%
TACAACAGCTTCGCCGGCGC+CGG - chr7.1:8188688-8188707 None:intergenic 65.0%
! GCGCCGGCGAAGCTGTTGTA+CGG + chr7.1:8188688-8188707 MS.gene20030:CDS 65.0%
!! GTCGGCAGCGTTGACGACGG+AGG - chr7.1:8188559-8188578 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 8188471 8191132 8188471 ID=MS.gene20030
chr7.1 mRNA 8188471 8191132 8188471 ID=MS.gene20030.t1;Parent=MS.gene20030
chr7.1 exon 8188471 8188940 8188471 ID=MS.gene20030.t1.exon1;Parent=MS.gene20030.t1
chr7.1 CDS 8188471 8188940 8188471 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8189053 8189078 8189053 ID=MS.gene20030.t1.exon2;Parent=MS.gene20030.t1
chr7.1 CDS 8189053 8189078 8189053 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8189193 8189223 8189193 ID=MS.gene20030.t1.exon3;Parent=MS.gene20030.t1
chr7.1 CDS 8189193 8189223 8189193 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8189326 8189413 8189326 ID=MS.gene20030.t1.exon4;Parent=MS.gene20030.t1
chr7.1 CDS 8189326 8189413 8189326 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8189602 8189747 8189602 ID=MS.gene20030.t1.exon5;Parent=MS.gene20030.t1
chr7.1 CDS 8189602 8189747 8189602 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8189889 8189933 8189889 ID=MS.gene20030.t1.exon6;Parent=MS.gene20030.t1
chr7.1 CDS 8189889 8189933 8189889 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8190069 8190172 8190069 ID=MS.gene20030.t1.exon7;Parent=MS.gene20030.t1
chr7.1 CDS 8190069 8190172 8190069 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8190373 8190515 8190373 ID=MS.gene20030.t1.exon8;Parent=MS.gene20030.t1
chr7.1 CDS 8190373 8190515 8190373 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8190642 8190755 8190642 ID=MS.gene20030.t1.exon9;Parent=MS.gene20030.t1
chr7.1 CDS 8190642 8190755 8190642 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
chr7.1 exon 8190884 8191132 8190884 ID=MS.gene20030.t1.exon10;Parent=MS.gene20030.t1
chr7.1 CDS 8190884 8191132 8190884 ID=cds.MS.gene20030.t1;Parent=MS.gene20030.t1
Gene Sequence

>MS.gene20030

ATGTTGGATCTCAATCTCAACGCTGATTCCTCTCTTCCACTCTCCTCCGTCGTTCAGATGGAAGATTCATCCGGTAGCTTTGATTCCTCCGTCGTCAACGCTGCCGACACTGAAGATTCATGCTCTTACGGCGATGCATACGGTTACAACTTCGCCATACTCAACAACAACGCTTCCGTCGACAACGGTACTATCTCCGGTGAGCTTCAACACTCCGGCGCCGGCGAAGCTGTTGTACGGACAATTCAGCTTTTTCCAGCAAACGAATCGGGAATGATCTCGCCTTCTGAGAGTAGAGGATGGATGAATTTCTCGTCCGATCATCACGGTGGCGTACCGGAGCAGACTGTTGTTGTTGCTCCACGACCTCAGCCGGTGAAGAAGAGTCGTCGGGGACCGAGGTCACGAAGCTCGCAGTATCGTGGTGTTACTTTCTACCGGAGAACTGGACGTTGGGAATCGCATATTTGGTTAGCATTTCGCAATGGATCATTCTGTTTCTTCGTAGTTCAGTTCATTCAGTTTAGTTTTCACTTTCTGCATTTTTGTTTCTGAATCCACTCTGTTTTCGTTTTTCTGTAGGGATTGTGGAAAACAAGTATACTTAGGTGAGAAATCAACACTGATTAAGTGATTATTTGAGACGATTTCGTTGTTTTATTTTACGTTCTTGATCGTTCTTCCTTTTTTTAGATTTTGATCTTTGAAATTTAATTTTTCAGGTGGATTTGATACCGCGCATGCTGCGGCAAGGTAATTTTCATATTTGAATATTTTTTATATATTAATTATTTTTTTAAATATTGAATGTTTTTCATGGATTTATTTTTGTTTTTTTGTATGATTTATTTTCAGAGCATATGATCGAGCTGCGATAAAGTTTCGTGGCGTCGATGCGGATATTAATTTCAATGTCAGTGATTATGATGAAGATATAAAGCAGGTTTGTGAGAATTTGTTATTACTAATGTTGTGTTGATCTTGTTTAAAATCTGATTGTAAGTTAGTATAATTGTAGCTTTAACTTTGATTCTATTGAAGTGTAGATGAGTAATTTTTGAGATGATCTATTTTAAAATTTGAATGTTAAGTTCTAATTGTAGCATAAACTTTGATTTTATTGTTGTGTAGATGAATAACTTTACAAAGGAAGAGTTTGTGCATATTCTGCGTAGACAGAGTACTGGTTTCTCAAGGGGAAGTTCTAAATACAGAGGAGTTACTCTGCATAAATGTGGACGATGGGAAGCTCGTATGGGACAGTTTCTTGGGAAGAAGTAAGAAAATCCTTTAGTTTAATTTGATTTATTAGTTGAACCTAAAATGACTGATTTGTGATTTATTTAACCATAAACCAGTATTTAGTTCTTAATTATATTGGTCTGGGTTGTTAATTATATTGGTCTGGGTTTATGTAGGTATATATATCTTGGGCTTTTTGATAGCGAGCTAGATGCTGCAAGGTTTTAATTTATCAATCATATATAGCAGGATATCTCAAATGAAATGCCTAATTAAATATTTTGGATCATTTCACTTTTATATTTTCTTTTCTACATGTTGTGTTATTGACTTGGTTGCTCTATTGATGATTACAGAGCGTATGATAAGGCTGCTATAAAATGCAATGGAAGGGAAGCAGTTACAAATTTTGAGGCTAGTTCTTATGAAGGAGAGTTAACTTCCCAGGCTGATAACGATGGTACTACTTATTGACCATTGAGGCTCACCTTTTTTTTGGTTTTATAATCAAATTGAATAAAACATCAATTTAGTCCATATAAACTATCACTCACTCTCTAATTTAGTCAGTCCTTAAAGTATGTCAATTAACCTTTACTATTAAGACTGGTGATTTAACTATGACGTGAAATGCAATGATGTATTTTGTGCTTTATTCAGATATCAAGCAGAATCTTGATCTGAACTTGGGTATAGCTCCTCCTTCTAACTCTGATGTTCAAATGATGAACATGCACCACAGTGGAAGTGGTTTGCAAGTTCAGCGTAACTGGGATGATATGCCTGTTGATAAGAGTGTAATGGTAAGGTCAAAAAAAAAAAAATTTTTTATATTAATTCTGCAATTCCTCATGCCCCTCTGCTCTTTCACATGAATTCAAAGTTTGCATACTTAACTTGCATCATATATTTTTAATCTGCGTTGATAGTTTGAGGACTCTGGGTCAAGATCACTCAATGTTCAGTCATCTTATGGCTTTCCCATAGCATCTGAGCATCAACCTCCAATCTGGAGTGGTACTAATTACTTTCCCGTGTGTAAGGTGAATACTAATTTTGCTTTAGTATCTTCTGCTAGTTATGAATTATCACTGTTCAATTTGATTTTGCTTCAATATCTTAAGAATCTTCTGCTGGTTATGAATTATGATTATTTGTGTTCTATTTACAGGAAAGAGCAATAGAGAATAGGATGGAATCTGCTCCCGTTCCAAATTGGGCATGGCAGCTCCAAAGTCCATATAATAATGGGTCAACTTTGATTCCACCTAATTTCTCTGCTGCAGCATCATCAGGATTCCCTTCAACAACAGCTGCGGATAGTCAGATTCATTTTCCAAACATAACATACCTCCACCCTCATTTTCCTACTCCAGCGACTAACTCCAGCCCCATACCCTCTTTCTACTGCAGGAGCTGA
Protein sequence

>MS.gene20030.t1

MLDLNLNADSSLPLSSVVQMEDSSGSFDSSVVNAADTEDSCSYGDAYGYNFAILNNNASVDNGTISGELQHSGAGEAVVRTIQLFPANESGMISPSESRGWMNFSSDHHGGVPEQTVVVAPRPQPVKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDRAAIKFRGVDADINFNVSDYDEDIKQMNNFTKEEFVHILRRQSTGFSRGSSKYRGVTLHKCGRWEARMGQFLGKKYIYLGLFDSELDAARAYDKAAIKCNGREAVTNFEASSYEGELTSQADNDDIKQNLDLNLGIAPPSNSDVQMMNMHHSGSGLQVQRNWDDMPVDKSVMFEDSGSRSLNVQSSYGFPIASEHQPPIWSGTNYFPVCKERAIENRMESAPVPNWAWQLQSPYNNGSTLIPPNFSAAASSGFPSTTAADSQIHFPNITYLHPHFPTPATNSSPIPSFYCRS