Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22900.t1 | XP_013450572.1 | 97.6 | 170 | 3 | 1 | 1 | 169 | 1 | 170 | 2.40E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22900.t1 | Q9LW68 | 75.6 | 131 | 26 | 3 | 23 | 150 | 44 | 171 | 1.9e-52 | 206.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22900.t1 | I3T2Z0 | 97.6 | 170 | 3 | 1 | 1 | 169 | 1 | 170 | 1.7e-91 | 344.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene22900 | 0.815659 | 8.25E-52 | -1.69E-46 |
| MS.gene050298 | MS.gene22900 | 0.831665 | 1.47E-55 | -1.69E-46 |
| MS.gene050563 | MS.gene22900 | 0.936808 | 9.59E-98 | -1.69E-46 |
| MS.gene050584 | MS.gene22900 | 0.83093 | 2.22E-55 | -1.69E-46 |
| MS.gene050585 | MS.gene22900 | 0.841782 | 3.86E-58 | -1.69E-46 |
| MS.gene050587 | MS.gene22900 | 0.832345 | 9.97E-56 | -1.69E-46 |
| MS.gene05143 | MS.gene22900 | 0.823713 | 1.19E-53 | -1.69E-46 |
| MS.gene051789 | MS.gene22900 | 0.830249 | 3.27E-55 | -1.69E-46 |
| MS.gene052142 | MS.gene22900 | 0.874943 | 4.55E-68 | -1.69E-46 |
| MS.gene052456 | MS.gene22900 | 0.803099 | 4.10E-49 | -1.69E-46 |
| MS.gene052698 | MS.gene22900 | 0.84214 | 3.10E-58 | -1.69E-46 |
| MS.gene053074 | MS.gene22900 | 0.808219 | 3.45E-50 | -1.69E-46 |
| MS.gene053383 | MS.gene22900 | 0.920694 | 9.25E-88 | -1.69E-46 |
| MS.gene053563 | MS.gene22900 | 0.80346 | 3.45E-49 | -1.69E-46 |
| MS.gene05456 | MS.gene22900 | 0.871286 | 7.67E-67 | -1.69E-46 |
| MS.gene055359 | MS.gene22900 | 0.879073 | 1.68E-69 | -1.69E-46 |
| MS.gene057022 | MS.gene22900 | 0.811408 | 7.11E-51 | -1.69E-46 |
| MS.gene058918 | MS.gene22900 | 0.835444 | 1.67E-56 | -1.69E-46 |
| MS.gene059006 | MS.gene22900 | 0.853412 | 2.43E-61 | -1.69E-46 |
| MS.gene059632 | MS.gene22900 | 0.806328 | 8.68E-50 | -1.69E-46 |
| MS.gene059768 | MS.gene22900 | 0.870545 | 1.35E-66 | -1.69E-46 |
| MS.gene060513 | MS.gene22900 | 0.828276 | 9.84E-55 | -1.69E-46 |
| MS.gene060691 | MS.gene22900 | 0.851357 | 9.35E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22900.t1 | MTR_7g115700 | 97.647 | 170 | 3 | 1 | 1 | 169 | 1 | 170 | 3.97e-124 | 346 |
| MS.gene22900.t1 | MTR_5g072510 | 72.263 | 137 | 31 | 4 | 26 | 158 | 36 | 169 | 1.07e-67 | 205 |
| MS.gene22900.t1 | MTR_4g094428 | 68.571 | 140 | 37 | 4 | 25 | 160 | 44 | 180 | 2.87e-64 | 197 |
| MS.gene22900.t1 | MTR_1g442860 | 73.438 | 128 | 28 | 3 | 27 | 151 | 65 | 189 | 4.07e-64 | 196 |
| MS.gene22900.t1 | MTR_2g016110 | 71.318 | 129 | 31 | 3 | 25 | 150 | 33 | 158 | 9.05e-63 | 191 |
| MS.gene22900.t1 | MTR_1g080210 | 73.288 | 146 | 33 | 3 | 27 | 169 | 40 | 182 | 2.10e-62 | 191 |
| MS.gene22900.t1 | MTR_2g092950 | 65.306 | 147 | 45 | 3 | 16 | 159 | 12 | 155 | 4.38e-62 | 189 |
| MS.gene22900.t1 | MTR_2g076400 | 68.217 | 129 | 35 | 3 | 25 | 150 | 31 | 156 | 5.15e-60 | 184 |
| MS.gene22900.t1 | MTR_1g075990 | 70.213 | 141 | 36 | 3 | 19 | 156 | 45 | 182 | 9.17e-60 | 184 |
| MS.gene22900.t1 | MTR_4g079930 | 66.667 | 129 | 37 | 3 | 25 | 150 | 27 | 152 | 1.79e-59 | 183 |
| MS.gene22900.t1 | MTR_7g097030 | 72.263 | 137 | 32 | 3 | 23 | 156 | 60 | 193 | 4.58e-59 | 183 |
| MS.gene22900.t1 | MTR_1g069825 | 73.684 | 133 | 29 | 3 | 27 | 156 | 52 | 181 | 1.49e-58 | 182 |
| MS.gene22900.t1 | MTR_3g031830 | 68.276 | 145 | 39 | 4 | 27 | 167 | 42 | 183 | 1.46e-56 | 177 |
| MS.gene22900.t1 | MTR_8g086350 | 64.865 | 148 | 43 | 5 | 25 | 167 | 29 | 172 | 3.45e-48 | 154 |
| MS.gene22900.t1 | MTR_1g114020 | 65.385 | 52 | 17 | 1 | 20 | 71 | 64 | 114 | 6.02e-17 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22900.t1 | AT5G58500 | 70.423 | 142 | 36 | 4 | 26 | 163 | 18 | 157 | 1.64e-67 | 203 |
| MS.gene22900.t1 | AT1G07090 | 65.493 | 142 | 43 | 3 | 26 | 164 | 30 | 168 | 1.49e-64 | 196 |
| MS.gene22900.t1 | AT5G28490 | 76.471 | 136 | 26 | 3 | 27 | 159 | 25 | 157 | 1.61e-62 | 191 |
| MS.gene22900.t1 | AT3G04510 | 70.714 | 140 | 35 | 3 | 27 | 163 | 33 | 169 | 8.56e-60 | 184 |
| MS.gene22900.t1 | AT2G42610 | 67.969 | 128 | 35 | 3 | 26 | 150 | 24 | 148 | 2.55e-59 | 182 |
| MS.gene22900.t1 | AT2G42610 | 67.969 | 128 | 35 | 3 | 26 | 150 | 24 | 148 | 2.55e-59 | 182 |
| MS.gene22900.t1 | AT2G31160 | 75.556 | 135 | 26 | 4 | 27 | 157 | 54 | 185 | 4.54e-58 | 181 |
| MS.gene22900.t1 | AT4G18610 | 65.116 | 129 | 38 | 3 | 26 | 150 | 36 | 161 | 2.18e-57 | 178 |
| MS.gene22900.t1 | AT3G23290 | 75.573 | 131 | 26 | 3 | 23 | 150 | 44 | 171 | 2.69e-57 | 178 |
| MS.gene22900.t1 | AT1G78815 | 63.566 | 129 | 41 | 3 | 25 | 150 | 38 | 163 | 2.96e-55 | 173 |
| MS.gene22900.t1 | AT1G16910 | 58.955 | 134 | 49 | 3 | 25 | 155 | 21 | 151 | 3.23e-52 | 164 |
Find 48 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCACTACCTTGATCAATT+TGG | 0.137888 | 7.4:-2026912 | MS.gene22900:CDS |
| AATCCAACTTGTCCTTTCTT+TGG | 0.215866 | 7.4:-2026873 | MS.gene22900:CDS |
| TGTTCCAGTCTCTCCTCTTT+TGG | 0.217847 | 7.4:+2027015 | None:intergenic |
| ATCCAACTTGTCCTTTCTTT+GGG | 0.324334 | 7.4:-2026872 | MS.gene22900:CDS |
| TTCACTACCTTGATCAATTT+GGG | 0.326576 | 7.4:-2026911 | MS.gene22900:CDS |
| TTGTCCTCTCCGTCAGGCTT+GGG | 0.343185 | 7.4:-2026823 | MS.gene22900:CDS |
| CTTGTCCTCTCCGTCAGGCT+TGG | 0.356689 | 7.4:-2026824 | MS.gene22900:CDS |
| CATGGTGGAAGTAACCCTTT+TGG | 0.395005 | 7.4:-2026747 | MS.gene22900:CDS |
| GCACATGGAGATGGAGGGTT+TGG | 0.411549 | 7.4:+2026846 | None:intergenic |
| CACTTCCATTGTAGCTGCTA+GGG | 0.441671 | 7.4:+2027075 | None:intergenic |
| GTCGATGGTTTCTTAAGTAC+TGG | 0.444811 | 7.4:+2026985 | None:intergenic |
| ATGTGCTTGTCCTCTCCGTC+AGG | 0.459665 | 7.4:-2026829 | MS.gene22900:CDS |
| TGATCAATTTGGGAAGACTA+AGG | 0.465045 | 7.4:-2026901 | MS.gene22900:CDS |
| GTGGTGGCAACAAGATCAAC+AGG | 0.470799 | 7.4:-2027046 | MS.gene22900:CDS |
| CTAACAGCGCCATCACCAAA+AGG | 0.486109 | 7.4:+2026732 | None:intergenic |
| GAGTTAGCTACCATAAGAAA+AGG | 0.502671 | 7.4:-2026665 | MS.gene22900:CDS |
| TAGTCTTCCCAAATTGATCA+AGG | 0.503689 | 7.4:+2026904 | None:intergenic |
| GTAGTTGTGATGTTGCGGTT+AGG | 0.503903 | 7.4:+2026636 | None:intergenic |
| AGAGCCAAAAGAGGAGAGAC+TGG | 0.512337 | 7.4:-2027019 | MS.gene22900:CDS |
| GCTTATGATCAAGAGATTCA+TGG | 0.517251 | 7.4:-2026765 | MS.gene22900:CDS |
| ATTGAAGCAGAAAGTGGTAC+TGG | 0.535622 | 7.4:+2026963 | None:intergenic |
| TCACTTCCATTGTAGCTGCT+AGG | 0.535763 | 7.4:+2027074 | None:intergenic |
| TTATACAAGAAATATGGCAT+TGG | 0.537262 | 7.4:-2027117 | None:intergenic |
| CTTTCTGCTTCAATGTGCAA+TGG | 0.545802 | 7.4:-2026954 | MS.gene22900:CDS |
| TGTCCTCTCCGTCAGGCTTG+GGG | 0.552431 | 7.4:-2026822 | MS.gene22900:CDS |
| GGTAGCCTTGATGCTCTCAT+CGG | 0.556787 | 7.4:-2026801 | MS.gene22900:CDS |
| AGAGGACAAGCACATGGAGA+TGG | 0.557220 | 7.4:+2026837 | None:intergenic |
| CAAATTGATCAAGGTAGTGA+AGG | 0.557585 | 7.4:+2026913 | None:intergenic |
| GGGTTTGGCATCCCAAAGAA+AGG | 0.560750 | 7.4:+2026861 | None:intergenic |
| ACAAACCCTAGCAGCTACAA+TGG | 0.562580 | 7.4:-2027080 | MS.gene22900:CDS |
| TTGCACATTGAAGCAGAAAG+TGG | 0.564133 | 7.4:+2026957 | None:intergenic |
| TAACAGCGCCATCACCAAAA+GGG | 0.569402 | 7.4:+2026733 | None:intergenic |
| CATGACAAATAGAAATCAAG+AGG | 0.576026 | 7.4:+2026602 | None:intergenic |
| GGACAAGCACATGGAGATGG+AGG | 0.578694 | 7.4:+2026840 | None:intergenic |
| ATCCCAAAGAAAGGACAAGT+TGG | 0.587746 | 7.4:+2026870 | None:intergenic |
| CAGAAAGTGGTACTGGTCGA+TGG | 0.588396 | 7.4:+2026970 | None:intergenic |
| GAGAAGTAGTTGTGATGTTG+CGG | 0.588969 | 7.4:+2026631 | None:intergenic |
| CAAGGCTACCCCAAGCCTGA+CGG | 0.597531 | 7.4:+2026814 | None:intergenic |
| ACAGGTACGAGAGCCAAAAG+AGG | 0.613687 | 7.4:-2027028 | MS.gene22900:CDS |
| TGACGGAGAGGACAAGCACA+TGG | 0.624357 | 7.4:+2026831 | None:intergenic |
| AATGGAAGTGATTCAAGTGG+TGG | 0.625867 | 7.4:-2027062 | MS.gene22900:CDS |
| GGCGACCGATGAGAGCATCA+AGG | 0.637841 | 7.4:+2026796 | None:intergenic |
| GACAAGCACATGGAGATGGA+GGG | 0.652537 | 7.4:+2026841 | None:intergenic |
| CTACCCCAAGCCTGACGGAG+AGG | 0.655871 | 7.4:+2026819 | None:intergenic |
| TACAATGGAAGTGATTCAAG+TGG | 0.673612 | 7.4:-2027065 | MS.gene22900:CDS |
| CTTGATCATAAGCAGCGCGG+AGG | 0.674942 | 7.4:+2026775 | None:intergenic |
| TATGATCAAGAGATTCATGG+TGG | 0.715975 | 7.4:-2026762 | MS.gene22900:CDS |
| TCTCTTGATCATAAGCAGCG+CGG | 0.775865 | 7.4:+2026772 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCACTACCTTGATCAATTT+GGG | - | chr7.4:2026810-2026829 | MS.gene22900:CDS | 30.0% | |
| AATCCAACTTGTCCTTTCTT+TGG | - | chr7.4:2026848-2026867 | MS.gene22900:CDS | 35.0% | |
| ATCCAACTTGTCCTTTCTTT+GGG | - | chr7.4:2026849-2026868 | MS.gene22900:CDS | 35.0% | |
| CAAATTGATCAAGGTAGTGA+AGG | + | chr7.4:2026811-2026830 | None:intergenic | 35.0% | |
| CTTCACTACCTTGATCAATT+TGG | - | chr7.4:2026809-2026828 | MS.gene22900:CDS | 35.0% | |
| GAGTTAGCTACCATAAGAAA+AGG | - | chr7.4:2027056-2027075 | MS.gene22900:CDS | 35.0% | |
| GCTTATGATCAAGAGATTCA+TGG | - | chr7.4:2026956-2026975 | MS.gene22900:CDS | 35.0% | |
| TACAATGGAAGTGATTCAAG+TGG | - | chr7.4:2026656-2026675 | MS.gene22900:CDS | 35.0% | |
| TAGTCTTCCCAAATTGATCA+AGG | + | chr7.4:2026820-2026839 | None:intergenic | 35.0% | |
| TATGATCAAGAGATTCATGG+TGG | - | chr7.4:2026959-2026978 | MS.gene22900:CDS | 35.0% | |
| TGATCAATTTGGGAAGACTA+AGG | - | chr7.4:2026820-2026839 | MS.gene22900:CDS | 35.0% | |
| ! | TAGGTCTCTTCCTTTTCTTA+TGG | + | chr7.4:2027069-2027088 | None:intergenic | 35.0% |
| ! | TGTTCGTGATTTTCAGTCAA+AGG | - | chr7.4:2027027-2027046 | MS.gene22900:CDS | 35.0% |
| AATGGAAGTGATTCAAGTGG+TGG | - | chr7.4:2026659-2026678 | MS.gene22900:CDS | 40.0% | |
| ATCCCAAAGAAAGGACAAGT+TGG | + | chr7.4:2026854-2026873 | None:intergenic | 40.0% | |
| ATTGAAGCAGAAAGTGGTAC+TGG | + | chr7.4:2026761-2026780 | None:intergenic | 40.0% | |
| CTTTCTGCTTCAATGTGCAA+TGG | - | chr7.4:2026767-2026786 | MS.gene22900:CDS | 40.0% | |
| GAGAAGTAGTTGTGATGTTG+CGG | + | chr7.4:2027093-2027112 | None:intergenic | 40.0% | |
| GTCGATGGTTTCTTAAGTAC+TGG | + | chr7.4:2026739-2026758 | None:intergenic | 40.0% | |
| TTGCACATTGAAGCAGAAAG+TGG | + | chr7.4:2026767-2026786 | None:intergenic | 40.0% | |
| ! | GATTTTCAGTCAAAGGCTAG+AGG | - | chr7.4:2027034-2027053 | MS.gene22900:CDS | 40.0% |
| ACAAACCCTAGCAGCTACAA+TGG | - | chr7.4:2026641-2026660 | MS.gene22900:CDS | 45.0% | |
| CACTTCCATTGTAGCTGCTA+GGG | + | chr7.4:2026649-2026668 | None:intergenic | 45.0% | |
| GTAGTTGTGATGTTGCGGTT+AGG | + | chr7.4:2027088-2027107 | None:intergenic | 45.0% | |
| TAACAGCGCCATCACCAAAA+GGG | + | chr7.4:2026991-2027010 | None:intergenic | 45.0% | |
| TCACTTCCATTGTAGCTGCT+AGG | + | chr7.4:2026650-2026669 | None:intergenic | 45.0% | |
| TCTCTTGATCATAAGCAGCG+CGG | + | chr7.4:2026952-2026971 | None:intergenic | 45.0% | |
| ! | GGAAGTAACCCTTTTGGTGA+TGG | - | chr7.4:2026980-2026999 | MS.gene22900:CDS | 45.0% |
| ! | TGTTCCAGTCTCTCCTCTTT+TGG | + | chr7.4:2026709-2026728 | None:intergenic | 45.0% |
| !! | CATGGTGGAAGTAACCCTTT+TGG | - | chr7.4:2026974-2026993 | MS.gene22900:CDS | 45.0% |
| ACAGGTACGAGAGCCAAAAG+AGG | - | chr7.4:2026693-2026712 | MS.gene22900:CDS | 50.0% | |
| AGAGCCAAAAGAGGAGAGAC+TGG | - | chr7.4:2026702-2026721 | MS.gene22900:CDS | 50.0% | |
| AGAGGACAAGCACATGGAGA+TGG | + | chr7.4:2026887-2026906 | None:intergenic | 50.0% | |
| CTAACAGCGCCATCACCAAA+AGG | + | chr7.4:2026992-2027011 | None:intergenic | 50.0% | |
| GACAAGCACATGGAGATGGA+GGG | + | chr7.4:2026883-2026902 | None:intergenic | 50.0% | |
| GGGTTTGGCATCCCAAAGAA+AGG | + | chr7.4:2026863-2026882 | None:intergenic | 50.0% | |
| GTGGTGGCAACAAGATCAAC+AGG | - | chr7.4:2026675-2026694 | MS.gene22900:CDS | 50.0% | |
| ! | GGTAGCCTTGATGCTCTCAT+CGG | - | chr7.4:2026920-2026939 | MS.gene22900:CDS | 50.0% |
| !! | CAGAAAGTGGTACTGGTCGA+TGG | + | chr7.4:2026754-2026773 | None:intergenic | 50.0% |
| !!! | CTTTTGGTGATGGCGCTGTT+AGG | - | chr7.4:2026990-2027009 | MS.gene22900:CDS | 50.0% |
| ATGTGCTTGTCCTCTCCGTC+AGG | - | chr7.4:2026892-2026911 | MS.gene22900:CDS | 55.0% | |
| CTTGATCATAAGCAGCGCGG+AGG | + | chr7.4:2026949-2026968 | None:intergenic | 55.0% | |
| GCACATGGAGATGGAGGGTT+TGG | + | chr7.4:2026878-2026897 | None:intergenic | 55.0% | |
| GGACAAGCACATGGAGATGG+AGG | + | chr7.4:2026884-2026903 | None:intergenic | 55.0% | |
| TGACGGAGAGGACAAGCACA+TGG | + | chr7.4:2026893-2026912 | None:intergenic | 55.0% | |
| TTGTCCTCTCCGTCAGGCTT+GGG | - | chr7.4:2026898-2026917 | MS.gene22900:CDS | 55.0% | |
| CAAGGCTACCCCAAGCCTGA+CGG | + | chr7.4:2026910-2026929 | None:intergenic | 60.0% | |
| CTTGTCCTCTCCGTCAGGCT+TGG | - | chr7.4:2026897-2026916 | MS.gene22900:CDS | 60.0% | |
| TGTCCTCTCCGTCAGGCTTG+GGG | - | chr7.4:2026899-2026918 | MS.gene22900:CDS | 60.0% | |
| !! | GGCGACCGATGAGAGCATCA+AGG | + | chr7.4:2026928-2026947 | None:intergenic | 60.0% |
| CTACCCCAAGCCTGACGGAG+AGG | + | chr7.4:2026905-2026924 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 2026617 | 2027126 | 2026617 | ID=MS.gene22900 |
| chr7.4 | mRNA | 2026617 | 2027126 | 2026617 | ID=MS.gene22900.t1;Parent=MS.gene22900 |
| chr7.4 | exon | 2026617 | 2027126 | 2026617 | ID=MS.gene22900.t1.exon1;Parent=MS.gene22900.t1 |
| chr7.4 | CDS | 2026617 | 2027126 | 2026617 | ID=cds.MS.gene22900.t1;Parent=MS.gene22900.t1 |
| Gene Sequence |
| Protein sequence |
