AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23983


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23983.t1 MTR_7g098290 100.000 140 0 0 1 140 1 140 1.96e-99 281
MS.gene23983.t1 MTR_1g069585 100.000 140 0 0 1 140 1 140 1.96e-99 281
MS.gene23983.t1 MTR_7g098290 100.000 125 0 0 16 140 1 125 4.58e-88 252
MS.gene23983.t1 MTR_1g026090 80.000 135 12 1 2 136 42 161 7.11e-70 208
MS.gene23983.t1 MTR_5g054560 67.200 125 17 1 16 140 1 101 4.13e-52 160
MS.gene23983.t1 MTR_5g464620 87.755 49 6 0 14 62 16 64 1.08e-24 90.5
MS.gene23983.t1 MTR_2g070580 90.244 41 4 0 100 140 87 127 1.94e-20 81.3
MS.gene23983.t1 MTR_1779s0020 37.391 115 64 3 26 136 8 118 3.53e-16 70.1
MS.gene23983.t1 MTR_4g051390 37.391 115 64 3 26 136 8 118 6.24e-16 69.3
MS.gene23983.t1 MTR_2g087550 37.736 106 59 2 26 128 8 109 2.08e-14 67.4
MS.gene23983.t1 MTR_0002s0780 35.246 122 72 3 23 140 132 250 2.22e-14 67.8
MS.gene23983.t1 MTR_4g066540 72.500 40 7 1 100 139 54 89 7.52e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23983.t1 AT3G04400 97.143 140 4 0 1 140 1 140 2.33e-97 276
MS.gene23983.t1 AT2G33370 97.143 140 4 0 1 140 1 140 2.33e-97 276
MS.gene23983.t1 AT1G04480 97.143 140 4 0 1 140 1 140 2.33e-97 276
MS.gene23983.t1 AT3G04400 98.400 125 2 0 16 140 1 125 1.18e-86 248
MS.gene23983.t1 AT2G33370 98.400 125 2 0 16 140 1 125 1.18e-86 248
MS.gene23983.t1 ATCG00780 34.783 115 67 3 26 136 8 118 1.22e-14 66.2

Find 48 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGGAATGTGCTGATCTT+TGG 0.329007 1.3:+49028523 MS.gene23983:CDS
ATACCTTTCATTTCTCCCTT+AGG 0.394994 1.3:-49028174 None:intergenic
TCAGCACATTCCTTAGCAAT+TGG 0.405879 1.3:-49028516 None:intergenic
GAACAAGTTCCGTATGTCAC+TGG 0.413902 1.3:+49027051 MS.gene23983:CDS
ACATGAAGACACCGTCCTTT+CGG 0.413999 1.3:-49027285 None:intergenic
CCTACCCTTGATTCCCTTCA+CGG 0.419579 1.3:-49027140 None:intergenic
AAAACCTTCTTCCTGAGATC+AGG 0.427356 1.3:-49027226 None:intergenic
CGTCTTCCTTCAGCTTGTGC+TGG 0.450748 1.3:+49027169 MS.gene23983:CDS
TTAGGTCGTGGTGGTTCCGC+GGG 0.471837 1.3:+49027028 MS.gene23983:intron
TTCAGCTTGTGCTGGTGACA+TGG 0.489849 1.3:+49027177 MS.gene23983:CDS
CATCTCCGTGAAGGGAATCA+AGG 0.491257 1.3:+49027135 MS.gene23983:CDS
TTGGCAGCACTTGCAATCCT+AGG 0.493302 1.3:-49028546 None:intergenic
CAGTTCACCGTCGCTGCCAC+TGG 0.502403 1.3:-49027079 None:intergenic
CGCTGACGAACAATGACAGC+TGG 0.509289 1.3:-49027250 None:intergenic
AGAAGGGAAAGCCTGATCTC+AGG 0.525390 1.3:+49027215 MS.gene23983:CDS
TTGTGCTGGTGACATGGTGA+TGG 0.531038 1.3:+49027183 MS.gene23983:CDS
AATGTGCTGATCTTTGGCCT+AGG 0.543147 1.3:+49028529 MS.gene23983:CDS
ACACCGTCCTTTCGGCGCCA+TGG 0.546165 1.3:-49027277 None:intergenic
ATTGTAGGTTCTGCTATCAC+CGG 0.549900 1.3:+49028492 MS.gene23983:intron
AACAAGTTCCGTATGTCACT+GGG 0.554811 1.3:+49027052 MS.gene23983:CDS
GGTGATGGCTACTGTGAAGA+AGG 0.561037 1.3:+49027198 MS.gene23983:CDS
CAGAATCCACACTCATACAA+TGG 0.562785 1.3:-49028571 None:intergenic
ATTAGGTCGTGGTGGTTCCG+CGG 0.570592 1.3:+49027027 MS.gene23983:intron
CTGGTAGACCCAGTGACATA+CGG 0.574663 1.3:-49027060 None:intergenic
AAGCCATGGCGCCGAAAGGA+CGG 0.575094 1.3:+49027274 MS.gene23983:CDS
AAAGCCTGATCTCAGGAAGA+AGG 0.579578 1.3:+49027222 MS.gene23983:CDS
TGGTGTCATTGTGAATCCTA+AGG 0.581900 1.3:+49028158 MS.gene23983:CDS
GGGTCTACCAGTGGCAGCGA+CGG 0.583652 1.3:+49027072 MS.gene23983:CDS
CCACACTCATACAATGGCAT+TGG 0.586391 1.3:-49028565 None:intergenic
CTGTACATCATCTCCGTGAA+GGG 0.589573 1.3:+49027127 MS.gene23983:CDS
ATCTCCGTGAAGGGAATCAA+GGG 0.592804 1.3:+49027136 MS.gene23983:CDS
ACATTCCTTAGCAATTGGAC+CGG 0.593816 1.3:-49028511 None:intergenic
AATCCTAAGGGAGAAATGAA+AGG 0.599885 1.3:+49028171 MS.gene23983:CDS
TCTGTACATCATCTCCGTGA+AGG 0.600106 1.3:+49027126 MS.gene23983:CDS
TTGTTCGTCAGCGTAAGCCA+TGG 0.601755 1.3:+49027260 MS.gene23983:CDS
GCGTAAGCCATGGCGCCGAA+AGG 0.602704 1.3:+49027270 MS.gene23983:CDS
TATCACCGGTCCAATTGCTA+AGG 0.605693 1.3:+49028506 MS.gene23983:CDS
ATGTCACCAGCACAAGCTGA+AGG 0.618315 1.3:-49027175 None:intergenic
AACGCAACCATGTCGAAGAG+AGG 0.636545 1.3:+49026932 None:intergenic
GGTGTCTTCATGTACTTCGA+AGG 0.643590 1.3:+49027295 MS.gene23983:CDS
GTGAACTGTGCTGATAACAC+TGG 0.644053 1.3:+49027094 MS.gene23983:CDS
CCGTGAAGGGAATCAAGGGT+AGG 0.651559 1.3:+49027140 MS.gene23983:CDS
GGTGTCATTGTGAATCCTAA+GGG 0.659767 1.3:+49028159 MS.gene23983:CDS
TATGTCACTGGGTCTACCAG+TGG 0.667978 1.3:+49027063 MS.gene23983:CDS
GTGATGGCTACTGTGAAGAA+GGG 0.669945 1.3:+49027199 MS.gene23983:CDS
GTACATACCTCTCTTCGACA+TGG 0.681454 1.3:-49026939 None:intergenic
TAGGTCGTGGTGGTTCCGCG+GGG 0.708397 1.3:+49027029 MS.gene23983:intron
CATACGGAACTTGTTCCCCG+CGG 0.790506 1.3:-49027044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAATCAATTAGACAAAA+CGG - chr1.3:49028429-49028448 None:intergenic 20.0%
!! ATAATTGATTATGACATGTT+TGG - chr1.3:49027826-49027845 None:intergenic 20.0%
!! ATATCAATTCAATCATTGTA+CGG + chr1.3:49028008-49028027 MS.gene23983:intron 20.0%
!! TTTAACAGAAATCAATTTCA+AGG - chr1.3:49027566-49027585 None:intergenic 20.0%
!! TTTCTATGTAGTAAATACTT+AGG + chr1.3:49028216-49028235 MS.gene23983:intron 20.0%
!!! TACACTTTTAGAATCAAATT+TGG + chr1.3:49027845-49027864 MS.gene23983:intron 20.0%
!!! TTTTAATTATGTCTTAATGC+CGG + chr1.3:49028092-49028111 MS.gene23983:intron 20.0%
! AATTGATATTAGCTAAAGAG+AGG - chr1.3:49027997-49028016 None:intergenic 25.0%
! CTCTTGTTAATATTTGGTAT+TGG + chr1.3:49028119-49028138 MS.gene23983:intron 25.0%
!! AAATATTAACAAGAGTACAC+CGG - chr1.3:49028114-49028133 None:intergenic 25.0%
!! AGTGAGTTGAACATAAAATT+TGG + chr1.3:49027727-49027746 MS.gene23983:intron 25.0%
!! CTATTTATTGGAAGTTTTTG+AGG + chr1.3:49027888-49027907 MS.gene23983:intron 25.0%
!! GTGAGTTGAACATAAAATTT+GGG + chr1.3:49027728-49027747 MS.gene23983:intron 25.0%
AACATATTTGCATGAGCATT+AGG - chr1.3:49028243-49028262 None:intergenic 30.0%
AATTAGACAAAACGGTACAA+AGG - chr1.3:49028421-49028440 None:intergenic 30.0%
ACATTGTAACAATAGCCAAA+AGG - chr1.3:49028320-49028339 None:intergenic 30.0%
AGAACAATTGAATCTGAGAA+AGG - chr1.3:49026978-49026997 None:intergenic 30.0%
GGTAACTTGAAGCTATTTAT+TGG + chr1.3:49027876-49027895 MS.gene23983:intron 30.0%
GGTGTACTCTTGTTAATATT+TGG + chr1.3:49028113-49028132 MS.gene23983:intron 30.0%
TAGCAAACTAACAAAAAGAC+AGG - chr1.3:49028390-49028409 None:intergenic 30.0%
! ATTGATTCATTGCTGGTATT+CGG + chr1.3:49028438-49028457 MS.gene23983:intron 30.0%
! TCTGATTGGTCATTCATATT+GGG + chr1.3:49027409-49027428 MS.gene23983:intron 30.0%
! TGTATCCCTATTTTCTGATT+AGG + chr1.3:49027010-49027029 MS.gene23983:intron 30.0%
! TTCTGATTGGTCATTCATAT+TGG + chr1.3:49027408-49027427 MS.gene23983:intron 30.0%
! TTGTCTAATTGATTCATTGC+TGG + chr1.3:49028431-49028450 MS.gene23983:intron 30.0%
!! TTTTGGCTATTGTTACAATG+TGG + chr1.3:49028319-49028338 MS.gene23983:intron 30.0%
!!! TACATTTTAGAATCAAGAGC+AGG + chr1.3:49027759-49027778 MS.gene23983:intron 30.0%
AATCAAAACACCACACACAT+AGG - chr1.3:49027347-49027366 None:intergenic 35.0%
AATCCTAAGGGAGAAATGAA+AGG + chr1.3:49028171-49028190 MS.gene23983:CDS 35.0%
AGAATCAAATTTGGTTGCTC+TGG + chr1.3:49027854-49027873 MS.gene23983:intron 35.0%
ATACCTTTCATTTCTCCCTT+AGG - chr1.3:49028177-49028196 None:intergenic 35.0%
CAAACTAACAAAAAGACAGG+TGG - chr1.3:49028387-49028406 None:intergenic 35.0%
CACGACCTAATCAGAAAATA+GGG - chr1.3:49027018-49027037 None:intergenic 35.0%
GAATCAAATTTGGTTGCTCT+GGG + chr1.3:49027855-49027874 MS.gene23983:intron 35.0%
TATGTCATGCTGTTCATTGT+AGG + chr1.3:49028477-49028496 MS.gene23983:intron 35.0%
! CTGATTGGTCATTCATATTG+GGG + chr1.3:49027410-49027429 MS.gene23983:intron 35.0%
!! TTGGTTTTTGCAGATAATGC+TGG + chr1.3:49028138-49028157 MS.gene23983:intron 35.0%
AAAACCTTCTTCCTGAGATC+AGG - chr1.3:49027229-49027248 None:intergenic 40.0%
AACAAGTTCCGTATGTCACT+GGG + chr1.3:49027052-49027071 MS.gene23983:CDS 40.0%
ACATTCCTTAGCAATTGGAC+CGG - chr1.3:49028514-49028533 None:intergenic 40.0%
ATTGTAGGTTCTGCTATCAC+CGG + chr1.3:49028492-49028511 MS.gene23983:intron 40.0%
CCACGACCTAATCAGAAAAT+AGG - chr1.3:49027019-49027038 None:intergenic 40.0%
GATTGTTGTACCTATGTGTG+TGG + chr1.3:49027334-49027353 MS.gene23983:intron 40.0%
TATGACATGTTTGGTTGCTC+TGG - chr1.3:49027817-49027836 None:intergenic 40.0%
TCAGCACATTCCTTAGCAAT+TGG - chr1.3:49028519-49028538 None:intergenic 40.0%
TGAGTGATCTGTGTTCTGAT+TGG + chr1.3:49027395-49027414 MS.gene23983:intron 40.0%
! ATTTTCTGATTAGGTCGTGG+TGG + chr1.3:49027019-49027038 MS.gene23983:intron 40.0%
! CACATCATGCTGTTTAAGAC+TGG - chr1.3:49027457-49027476 None:intergenic 40.0%
! CCTATTTTCTGATTAGGTCG+TGG + chr1.3:49027016-49027035 MS.gene23983:intron 40.0%
! CTAAGGAATGTGCTGATCTT+TGG + chr1.3:49028523-49028542 MS.gene23983:CDS 40.0%
!! GGTGTCATTGTGAATCCTAA+GGG + chr1.3:49028159-49028178 MS.gene23983:CDS 40.0%
!! TGGTGTCATTGTGAATCCTA+AGG + chr1.3:49028158-49028177 MS.gene23983:CDS 40.0%
AAAGCCTGATCTCAGGAAGA+AGG + chr1.3:49027222-49027241 MS.gene23983:CDS 45.0%
AACGAGATTTACCAGCAGAC+AGG - chr1.3:49027501-49027520 None:intergenic 45.0%
ACATGAAGACACCGTCCTTT+CGG - chr1.3:49027288-49027307 None:intergenic 45.0%
ATCTCCGTGAAGGGAATCAA+GGG + chr1.3:49027136-49027155 MS.gene23983:CDS 45.0%
CTGTACATCATCTCCGTGAA+GGG + chr1.3:49027127-49027146 MS.gene23983:CDS 45.0%
GAACAAGTTCCGTATGTCAC+TGG + chr1.3:49027051-49027070 MS.gene23983:CDS 45.0%
GGTGTCTTCATGTACTTCGA+AGG + chr1.3:49027295-49027314 MS.gene23983:CDS 45.0%
GTGATGGCTACTGTGAAGAA+GGG + chr1.3:49027199-49027218 MS.gene23983:CDS 45.0%
TATCACCGGTCCAATTGCTA+AGG + chr1.3:49028506-49028525 MS.gene23983:CDS 45.0%
TCTGTACATCATCTCCGTGA+AGG + chr1.3:49027126-49027145 MS.gene23983:CDS 45.0%
! AATGTGCTGATCTTTGGCCT+AGG + chr1.3:49028529-49028548 MS.gene23983:CDS 45.0%
! ACACTTGCTTCAGTGCCTTT+TGG + chr1.3:49028302-49028321 MS.gene23983:intron 45.0%
! GTGAACTGTGCTGATAACAC+TGG + chr1.3:49027094-49027113 MS.gene23983:CDS 45.0%
AGAAGGGAAAGCCTGATCTC+AGG + chr1.3:49027215-49027234 MS.gene23983:CDS 50.0%
ATGTCACCAGCACAAGCTGA+AGG - chr1.3:49027178-49027197 None:intergenic 50.0%
CATCTCCGTGAAGGGAATCA+AGG + chr1.3:49027135-49027154 MS.gene23983:CDS 50.0%
CCTACCCTTGATTCCCTTCA+CGG - chr1.3:49027143-49027162 None:intergenic 50.0%
CTGGTAGACCCAGTGACATA+CGG - chr1.3:49027063-49027082 None:intergenic 50.0%
TATGTCACTGGGTCTACCAG+TGG + chr1.3:49027063-49027082 MS.gene23983:CDS 50.0%
TCATTGTGCTACCTGTCTGC+TGG + chr1.3:49027487-49027506 MS.gene23983:intron 50.0%
TTGGCAGCACTTGCAATCCT+AGG - chr1.3:49028549-49028568 None:intergenic 50.0%
TTGTTCGTCAGCGTAAGCCA+TGG + chr1.3:49027260-49027279 MS.gene23983:CDS 50.0%
! GGTGATGGCTACTGTGAAGA+AGG + chr1.3:49027198-49027217 MS.gene23983:CDS 50.0%
! TTCAGCTTGTGCTGGTGACA+TGG + chr1.3:49027177-49027196 MS.gene23983:CDS 50.0%
! TTGTGCTGGTGACATGGTGA+TGG + chr1.3:49027183-49027202 MS.gene23983:CDS 50.0%
ATTAGGTCGTGGTGGTTCCG+CGG + chr1.3:49027027-49027046 MS.gene23983:intron 55.0%
CCGTGAAGGGAATCAAGGGT+AGG + chr1.3:49027140-49027159 MS.gene23983:CDS 55.0%
CGCTGACGAACAATGACAGC+TGG - chr1.3:49027253-49027272 None:intergenic 55.0%
CGTCTTCCTTCAGCTTGTGC+TGG + chr1.3:49027169-49027188 MS.gene23983:CDS 55.0%
! CATACGGAACTTGTTCCCCG+CGG - chr1.3:49027047-49027066 None:intergenic 55.0%
TTAGGTCGTGGTGGTTCCGC+GGG + chr1.3:49027028-49027047 MS.gene23983:intron 60.0%
! AAGCCATGGCGCCGAAAGGA+CGG + chr1.3:49027274-49027293 MS.gene23983:CDS 60.0%
ACACCGTCCTTTCGGCGCCA+TGG - chr1.3:49027280-49027299 None:intergenic 65.0%
CAGTTCACCGTCGCTGCCAC+TGG - chr1.3:49027082-49027101 None:intergenic 65.0%
GGGTCTACCAGTGGCAGCGA+CGG + chr1.3:49027072-49027091 MS.gene23983:CDS 65.0%
TAGGTCGTGGTGGTTCCGCG+GGG + chr1.3:49027029-49027048 MS.gene23983:intron 65.0%
! GCGTAAGCCATGGCGCCGAA+AGG + chr1.3:49027270-49027289 MS.gene23983:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 49026941 49028581 49026941 ID=MS.gene23983
chr1.3 mRNA 49026941 49028581 49026941 ID=MS.gene23983.t1;Parent=MS.gene23983
chr1.3 exon 49026941 49026953 49026941 ID=MS.gene23983.t1.exon1;Parent=MS.gene23983.t1
chr1.3 CDS 49026941 49026953 49026941 ID=cds.MS.gene23983.t1;Parent=MS.gene23983.t1
chr1.3 exon 49027032 49027316 49027032 ID=MS.gene23983.t1.exon2;Parent=MS.gene23983.t1
chr1.3 CDS 49027032 49027316 49027032 ID=cds.MS.gene23983.t1;Parent=MS.gene23983.t1
chr1.3 exon 49028151 49028192 49028151 ID=MS.gene23983.t1.exon3;Parent=MS.gene23983.t1
chr1.3 CDS 49028151 49028192 49028151 ID=cds.MS.gene23983.t1;Parent=MS.gene23983.t1
chr1.3 exon 49028499 49028581 49028499 ID=MS.gene23983.t1.exon4;Parent=MS.gene23983.t1
chr1.3 CDS 49028499 49028581 49028499 ID=cds.MS.gene23983.t1;Parent=MS.gene23983.t1
Gene Sequence

>MS.gene23983

ATGTCGAAGAGAGGTATGTACAGTGTTTCTGAATCCTTTCTCAGATTCAATTGTTCTCTTTTTCTGATTTGTATCCCTATTTTCTGATTAGGTCGTGGTGGTTCCGCGGGGAACAAGTTCCGTATGTCACTGGGTCTACCAGTGGCAGCGACGGTGAACTGTGCTGATAACACTGGAGCAAAGAATCTGTACATCATCTCCGTGAAGGGAATCAAGGGTAGGTTGAATCGTCTTCCTTCAGCTTGTGCTGGTGACATGGTGATGGCTACTGTGAAGAAGGGAAAGCCTGATCTCAGGAAGAAGGTTTTGCCAGCTGTCATTGTTCGTCAGCGTAAGCCATGGCGCCGAAAGGACGGTGTCTTCATGTACTTCGAAGGTAACAAACGCTGTTTTGATTGTTGTACCTATGTGTGTGGTGTTTTGATTTTGAAATGTTGCTTCTGTAAATTTATGATGAGTGATCTGTGTTCTGATTGGTCATTCATATTGGGGAAGCATATAACTGTGTTATTGCCAGTCTTAAACAGCATGATGTGTAATTTTGTTTCATTGTGCTACCTGTCTGCTGGTAAATCTCGTTTTATATATAAGTGTTGATTAAAATTGATTGAGTGAATTGATTCCTTGAAATTGATTTCTGTTAAAAGTGAGTTGAATCTAAAGTTATTTATGTCTAGATAACATTTATTTATAATTGATTGAGTGAATTGATTCCTTGAAATTGATTTCTGTTAAAAGTGAGTTGAATCTAAAGTTATTTATGTCTAGATAACATTTATTTATAAAAGTGAGTTGAACATAAAATTTGGGTGTGAAAATACATTTTAGAATCAAGAGCAGGAGAATCAATTCGAGTAGAAAATCAATTCTACTCCAGAGCAACCAAACATGTCATAATCAATTATACACTTTTAGAATCAAATTTGGTTGCTCTGGGTAACTTGAAGCTATTTATTGGAAGTTTTTGAGGCTAGAGTTGATTCTAACACTTAAACATGTCATAATCATTTTACTTAAACTCACTTTTAGCTCGAATTAATTTTACTAAATCAACCTCTCTTTAGCTAATATCAATTCAATCATTGTACGGAATTAATTCATTTAATATCAAATTTTGTCAACATAACTTATTCAATCTGTAGTGCTCTCAGTTTTAATTATGTCTTAATGCCGGTGTACTCTTGTTAATATTTGGTATTGGTTTTTGCAGATAATGCTGGTGTCATTGTGAATCCTAAGGGAGAAATGAAAGGTATCATTTATTCATTTATCTGATTTCTATGTAGTAAATACTTAGGACCTAATGCTCATGCAAATATGTTTACTTTCTTTTGTAATTTAAAGTGTAATATTAAATTAATACACTTGCTTCAGTGCCTTTTGGCTATTGTTACAATGTGGTAATGCTTCTGTGTTATTTTTTCACAAGAAAAAAACATTACACCACCTGTCTTTTTGTTAGTTTGCTACATTGTTACCTTTGTACCGTTTTGTCTAATTGATTCATTGCTGGTATTCGGTTCGTGTCTTGATGTCTATGTCATGCTGTTCATTGTAGGTTCTGCTATCACCGGTCCAATTGCTAAGGAATGTGCTGATCTTTGGCCTAGGATTGCAAGTGCTGCCAATGCCATTGTATGA

Protein sequence

>MS.gene23983.t1

MSKRGRGGSAGNKFRMSLGLPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACAGDMVMATVKKGKPDLRKKVLPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAITGPIAKECADLWPRIASAANAIV