Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24527.t1 | ABO33479.1 | 99.6 | 238 | 1 | 0 | 1 | 238 | 51 | 288 | 3.30E-131 | 477.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24527.t1 | O04135 | 86.5 | 244 | 26 | 3 | 1 | 238 | 155 | 397 | 3.1e-114 | 412.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24527.t1 | X2D315 | 99.6 | 238 | 1 | 0 | 1 | 238 | 142 | 379 | 2.4e-131 | 477.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene24527.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene24527 | 0.819032 | 1.44E-52 | -1.69E-46 |
| MS.gene049660 | MS.gene24527 | 0.827091 | 1.89E-54 | -1.69E-46 |
| MS.gene050297 | MS.gene24527 | 0.800312 | 1.53E-48 | -1.69E-46 |
| MS.gene05054 | MS.gene24527 | 0.818267 | 2.14E-52 | -1.69E-46 |
| MS.gene051478 | MS.gene24527 | 0.814017 | 1.91E-51 | -1.69E-46 |
| MS.gene051648 | MS.gene24527 | 0.987796 | 1.44E-171 | -1.69E-46 |
| MS.gene051660 | MS.gene24527 | 0.824715 | 6.95E-54 | -1.69E-46 |
| MS.gene051664 | MS.gene24527 | 0.813107 | 3.03E-51 | -1.69E-46 |
| MS.gene052150 | MS.gene24527 | 0.939921 | 5.63E-100 | -1.69E-46 |
| MS.gene052151 | MS.gene24527 | 0.936266 | 2.28E-97 | -1.69E-46 |
| MS.gene053536 | MS.gene24527 | 0.871144 | 8.55E-67 | -1.69E-46 |
| MS.gene053538 | MS.gene24527 | 0.865871 | 4.31E-65 | -1.69E-46 |
| MS.gene054999 | MS.gene24527 | 0.808228 | 3.43E-50 | -1.69E-46 |
| MS.gene055537 | MS.gene24527 | 0.820313 | 7.33E-53 | -1.69E-46 |
| MS.gene055764 | MS.gene24527 | 0.811856 | 5.68E-51 | -1.69E-46 |
| MS.gene056169 | MS.gene24527 | 0.821591 | 3.72E-53 | -1.69E-46 |
| MS.gene057004 | MS.gene24527 | 0.802036 | 6.79E-49 | -1.69E-46 |
| MS.gene057082 | MS.gene24527 | 0.822961 | 1.79E-53 | -1.69E-46 |
| MS.gene05894 | MS.gene24527 | 0.867466 | 1.34E-65 | -1.69E-46 |
| MS.gene059424 | MS.gene24527 | 0.870177 | 1.78E-66 | -1.69E-46 |
| MS.gene060519 | MS.gene24527 | 0.81555 | 8.73E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24527.t1 | MTR_1g017080 | 99.580 | 238 | 1 | 0 | 1 | 238 | 142 | 379 | 1.56e-179 | 498 |
| MS.gene24527.t1 | MTR_2g024390 | 58.515 | 229 | 81 | 3 | 1 | 225 | 140 | 358 | 1.39e-77 | 238 |
| MS.gene24527.t1 | MTR_5g033720 | 55.300 | 217 | 92 | 2 | 1 | 215 | 95 | 308 | 1.51e-73 | 226 |
| MS.gene24527.t1 | MTR_5g085860 | 56.422 | 218 | 81 | 3 | 1 | 214 | 69 | 276 | 1.24e-63 | 200 |
| MS.gene24527.t1 | MTR_1g084060 | 48.035 | 229 | 106 | 4 | 1 | 221 | 42 | 265 | 2.38e-61 | 193 |
| MS.gene24527.t1 | MTR_1g084060 | 48.035 | 229 | 106 | 4 | 1 | 221 | 42 | 265 | 4.70e-61 | 194 |
| MS.gene24527.t1 | MTR_2g461240 | 32.287 | 223 | 129 | 5 | 1 | 201 | 117 | 339 | 1.20e-28 | 111 |
| MS.gene24527.t1 | MTR_1g012960 | 32.719 | 217 | 126 | 4 | 5 | 201 | 204 | 420 | 1.99e-27 | 108 |
| MS.gene24527.t1 | MTR_5g011070 | 32.547 | 212 | 103 | 5 | 23 | 201 | 72 | 276 | 7.38e-27 | 105 |
| MS.gene24527.t1 | MTR_4g116545 | 31.839 | 223 | 130 | 5 | 1 | 201 | 93 | 315 | 9.90e-26 | 103 |
| MS.gene24527.t1 | MTR_4g116545 | 31.839 | 223 | 130 | 5 | 1 | 201 | 93 | 315 | 1.03e-25 | 102 |
| MS.gene24527.t1 | MTR_3g106400 | 33.862 | 189 | 104 | 4 | 34 | 201 | 167 | 355 | 9.76e-25 | 100 |
| MS.gene24527.t1 | MTR_3g106400 | 33.862 | 189 | 104 | 4 | 34 | 201 | 167 | 355 | 1.09e-24 | 100 |
| MS.gene24527.t1 | MTR_3g106400 | 33.862 | 189 | 104 | 4 | 34 | 201 | 167 | 355 | 2.13e-24 | 100 |
| MS.gene24527.t1 | MTR_7g065050 | 39.726 | 73 | 42 | 1 | 131 | 203 | 296 | 366 | 7.84e-12 | 64.7 |
| MS.gene24527.t1 | MTR_4g019450 | 43.243 | 74 | 39 | 2 | 130 | 203 | 351 | 421 | 1.08e-11 | 64.3 |
| MS.gene24527.t1 | MTR_4g051532 | 50.909 | 55 | 27 | 0 | 149 | 203 | 409 | 463 | 3.15e-11 | 63.2 |
| MS.gene24527.t1 | MTR_3g112290 | 49.091 | 55 | 28 | 0 | 149 | 203 | 355 | 409 | 3.20e-11 | 62.8 |
| MS.gene24527.t1 | MTR_1g023050 | 49.091 | 55 | 28 | 0 | 149 | 203 | 363 | 417 | 5.51e-11 | 62.4 |
| MS.gene24527.t1 | MTR_3g112300 | 49.091 | 55 | 28 | 0 | 149 | 203 | 352 | 406 | 7.98e-11 | 61.6 |
| MS.gene24527.t1 | MTR_3g112300 | 49.091 | 55 | 28 | 0 | 149 | 203 | 352 | 406 | 8.05e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24527.t1 | AT4G08150 | 82.305 | 243 | 37 | 5 | 1 | 238 | 157 | 398 | 4.89e-134 | 383 |
| MS.gene24527.t1 | AT1G62360 | 57.872 | 235 | 82 | 4 | 1 | 231 | 144 | 365 | 2.65e-75 | 233 |
| MS.gene24527.t1 | AT1G23380 | 55.349 | 215 | 89 | 3 | 1 | 213 | 108 | 317 | 5.81e-72 | 223 |
| MS.gene24527.t1 | AT1G23380 | 55.349 | 215 | 89 | 3 | 1 | 213 | 110 | 319 | 6.09e-72 | 223 |
| MS.gene24527.t1 | AT1G70510 | 52.113 | 213 | 99 | 1 | 1 | 213 | 92 | 301 | 1.47e-67 | 211 |
| MS.gene24527.t1 | AT1G70510 | 51.152 | 217 | 99 | 2 | 1 | 213 | 92 | 305 | 2.74e-65 | 205 |
| MS.gene24527.t1 | AT1G62990 | 34.021 | 194 | 93 | 5 | 36 | 201 | 89 | 275 | 4.33e-28 | 108 |
| MS.gene24527.t1 | AT5G25220 | 37.433 | 187 | 95 | 5 | 34 | 201 | 220 | 403 | 1.20e-27 | 109 |
| MS.gene24527.t1 | AT5G25220 | 37.433 | 187 | 95 | 5 | 34 | 201 | 220 | 403 | 1.37e-27 | 109 |
| MS.gene24527.t1 | AT5G11060 | 35.263 | 190 | 101 | 5 | 31 | 201 | 181 | 367 | 1.02e-26 | 106 |
| MS.gene24527.t1 | AT4G32040 | 29.204 | 226 | 131 | 4 | 1 | 201 | 141 | 362 | 1.08e-25 | 103 |
| MS.gene24527.t1 | AT4G32040 | 28.761 | 226 | 132 | 4 | 1 | 201 | 141 | 362 | 1.18e-25 | 103 |
| MS.gene24527.t1 | AT1G75430 | 49.091 | 55 | 28 | 0 | 149 | 203 | 209 | 263 | 9.51e-12 | 63.9 |
| MS.gene24527.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 149 | 203 | 358 | 412 | 1.24e-11 | 64.3 |
| MS.gene24527.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 149 | 203 | 358 | 412 | 1.24e-11 | 64.3 |
| MS.gene24527.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 149 | 203 | 358 | 412 | 1.24e-11 | 64.3 |
| MS.gene24527.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 149 | 203 | 358 | 412 | 1.24e-11 | 64.3 |
| MS.gene24527.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 149 | 203 | 358 | 412 | 1.24e-11 | 64.3 |
| MS.gene24527.t1 | AT2G27220 | 49.091 | 55 | 28 | 0 | 149 | 203 | 235 | 289 | 4.28e-11 | 62.4 |
| MS.gene24527.t1 | AT2G27220 | 49.091 | 55 | 28 | 0 | 149 | 203 | 235 | 289 | 4.28e-11 | 62.4 |
| MS.gene24527.t1 | AT5G02030 | 47.273 | 55 | 29 | 0 | 149 | 203 | 355 | 409 | 4.44e-11 | 62.8 |
| MS.gene24527.t1 | AT2G27220 | 49.091 | 55 | 28 | 0 | 149 | 203 | 253 | 307 | 5.33e-11 | 62.4 |
| MS.gene24527.t1 | AT1G75410 | 49.091 | 55 | 28 | 0 | 149 | 203 | 353 | 407 | 5.83e-11 | 62.4 |
| MS.gene24527.t1 | AT1G75410 | 49.091 | 55 | 28 | 0 | 149 | 203 | 353 | 407 | 5.83e-11 | 62.4 |
Find 58 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAGAAGACATGCAGTTTA+TGG | 0.215872 | 1.2:-5664823 | MS.gene24527:CDS |
| GCTGATGGTCCCTACCGTTT+AGG | 0.271349 | 1.2:-5664744 | MS.gene24527:CDS |
| ACCAGTTATTTATTTGCTTC+TGG | 0.277102 | 1.2:+5664877 | None:intergenic |
| ACAGAGTGCAGCTCTTTATA+TGG | 0.322707 | 1.2:-5664781 | MS.gene24527:CDS |
| ACCATGAATTGGTCTAGTTC+TGG | 0.338115 | 1.2:+5674873 | None:intergenic |
| CTGATGGTCCCTACCGTTTA+GGG | 0.343258 | 1.2:-5664743 | MS.gene24527:CDS |
| CTAGACAAAAGCTACTTAAT+TGG | 0.349186 | 1.2:-5665562 | MS.gene24527:CDS |
| ACCAAACGAGCCACCACTTC+CGG | 0.349272 | 1.2:+5674963 | None:intergenic |
| ACCGGAAGTGGTGGCTCGTT+TGG | 0.365361 | 1.2:-5674964 | MS.gene24527:CDS |
| ACCAGAAGCAAATAAATAAC+TGG | 0.406580 | 1.2:-5664878 | MS.gene24527:CDS |
| GAAAGGAAAATTACCTAAAG+AGG | 0.441916 | 1.2:-5665585 | MS.gene24527:CDS |
| AAATCCATAGCCTCTTGTAT+AGG | 0.443002 | 1.2:+5674604 | None:intergenic |
| GTAGCATTGGCTGAATCAAC+AGG | 0.445109 | 1.2:-5664906 | MS.gene24527:CDS |
| TTACAAATGGCCTTATCCTT+CGG | 0.445371 | 1.2:-5665528 | MS.gene24527:intron |
| ATCATAATCATGGCCCTAAA+CGG | 0.455352 | 1.2:+5664730 | None:intergenic |
| ATTGGCTGAATCAACAGGTT+TGG | 0.461797 | 1.2:-5664901 | MS.gene24527:CDS |
| TCCAGAACTAGACCAATTCA+TGG | 0.475218 | 1.2:-5674874 | MS.gene24527:intron |
| CACTTTGCAATGGACCCCTT+CGG | 0.484187 | 1.2:-5674552 | MS.gene24527:CDS |
| GGTGGGAATTGCATTACAAA+TGG | 0.484691 | 1.2:-5665541 | MS.gene24527:CDS |
| TCTGCTCGAGGATCAATCTC+TGG | 0.491728 | 1.2:+5665692 | None:intergenic |
| GATTGGAGCTCCACCGGAAG+TGG | 0.502488 | 1.2:-5674976 | MS.gene24527:CDS |
| GGACCCCTTCGGATCTTCCC+TGG | 0.503797 | 1.2:-5674541 | MS.gene24527:intron |
| TCAATCAAAGGAAGAGGCAC+TGG | 0.507111 | 1.2:-5664851 | MS.gene24527:CDS |
| TTATAACGTACCGAAGGATA+AGG | 0.509147 | 1.2:+5665518 | None:intergenic |
| CACTATATTTCTTCAGCAAG+TGG | 0.509181 | 1.2:+5665651 | None:intergenic |
| GACAAAAGCTACTTAATTGG+TGG | 0.509834 | 1.2:-5665559 | MS.gene24527:CDS |
| TTATATGGATGGTCATTACA+TGG | 0.510806 | 1.2:-5664766 | MS.gene24527:CDS |
| AAGGCCTATACAAGAGGCTA+TGG | 0.514686 | 1.2:-5674608 | MS.gene24527:CDS |
| TGGCTCGTTTGGTTGCGTCT+AGG | 0.518848 | 1.2:-5674953 | MS.gene24527:CDS |
| GAGGATCAAGAGAATAGTGG+TGG | 0.527205 | 1.2:-5665731 | MS.gene24527:CDS |
| AATTCACGGTCTTCTGCTCG+AGG | 0.528363 | 1.2:+5665680 | None:intergenic |
| CACTTGCTGAAGAAATATAG+TGG | 0.533238 | 1.2:-5665650 | MS.gene24527:CDS |
| GAAGAGGATCAAGAGAATAG+TGG | 0.545053 | 1.2:-5665734 | MS.gene24527:CDS |
| TATGTAGATTGGAGCTCCAC+CGG | 0.547316 | 1.2:-5674982 | None:intergenic |
| GGAATCAGAGAAGGTAGCAT+TGG | 0.547951 | 1.2:-5664919 | MS.gene24527:CDS |
| ATAAACTGCATGTCTTCTGA+TGG | 0.556878 | 1.2:+5664825 | None:intergenic |
| AGACATGCAGTTTATGGTGA+TGG | 0.558113 | 1.2:-5664817 | MS.gene24527:CDS |
| CAGCTAAATACACTTTGCAA+TGG | 0.562110 | 1.2:-5674562 | MS.gene24527:CDS |
| TAATCATGGCCCTAAACGGT+AGG | 0.564867 | 1.2:+5664734 | None:intergenic |
| TGGAGCTCCACCGGAAGTGG+TGG | 0.570424 | 1.2:-5674973 | MS.gene24527:CDS |
| GGTTCATCAATCAAAGGAAG+AGG | 0.570775 | 1.2:-5664857 | MS.gene24527:CDS |
| AGTGCAGCTCTTTATATGGA+TGG | 0.581356 | 1.2:-5664777 | MS.gene24527:CDS |
| ATAACTGGTTCATCAATCAA+AGG | 0.592016 | 1.2:-5664863 | MS.gene24527:CDS |
| ACAAAAGCTACTTAATTGGT+GGG | 0.596461 | 1.2:-5665558 | MS.gene24527:CDS |
| ACTCACCAGGGAAGATCCGA+AGG | 0.608503 | 1.2:+5674536 | None:intergenic |
| ATTAACAAGGCCTATACAAG+AGG | 0.622954 | 1.2:-5674614 | MS.gene24527:CDS |
| AGGCGTTGGTTCATCAGAAG+AGG | 0.623533 | 1.2:-5665750 | MS.gene24527:CDS |
| AATCATGGCCCTAAACGGTA+GGG | 0.623950 | 1.2:+5664735 | None:intergenic |
| ATGCAGTTTATGGTGATGGA+TGG | 0.624056 | 1.2:-5664813 | MS.gene24527:CDS |
| ATATAAAGAGCTGCACTCTG+TGG | 0.627158 | 1.2:+5664783 | None:intergenic |
| AAACGAGCCACCACTTCCGG+TGG | 0.627824 | 1.2:+5674966 | None:intergenic |
| GATGGTCATTACATGGCTGA+TGG | 0.643940 | 1.2:-5664759 | MS.gene24527:CDS |
| CACTATATAGGAATCAGAGA+AGG | 0.647456 | 1.2:-5664928 | MS.gene24527:intron |
| CATGCTTGTGAAGTATAGAG+AGG | 0.648293 | 1.2:-5674638 | MS.gene24527:CDS |
| CTCACCAGGGAAGATCCGAA+GGG | 0.678902 | 1.2:+5674537 | None:intergenic |
| CTAGGCAAGAGTTTGAAGCA+AGG | 0.689197 | 1.2:-5674935 | MS.gene24527:CDS |
| AGTATAGAGAGGAATTAACA+AGG | 0.714149 | 1.2:-5674627 | MS.gene24527:CDS |
| TCACCAGGGAAGATCCGAAG+GGG | 0.724320 | 1.2:+5674538 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 5664737 | 5674997 | 5664737 | ID=MS.gene24527 |
| chr1.2 | mRNA | 5664737 | 5674997 | 5664737 | ID=MS.gene24527.t1;Parent=MS.gene24527 |
| chr1.2 | exon | 5674875 | 5674997 | 5674875 | ID=MS.gene24527.t1.exon1;Parent=MS.gene24527.t1 |
| chr1.2 | CDS | 5674875 | 5674997 | 5674875 | ID=cds.MS.gene24527.t1;Parent=MS.gene24527.t1 |
| chr1.2 | exon | 5674542 | 5674674 | 5674542 | ID=MS.gene24527.t1.exon2;Parent=MS.gene24527.t1 |
| chr1.2 | CDS | 5674542 | 5674674 | 5674542 | ID=cds.MS.gene24527.t1;Parent=MS.gene24527.t1 |
| chr1.2 | exon | 5665529 | 5665785 | 5665529 | ID=MS.gene24527.t1.exon3;Parent=MS.gene24527.t1 |
| chr1.2 | CDS | 5665529 | 5665785 | 5665529 | ID=cds.MS.gene24527.t1;Parent=MS.gene24527.t1 |
| chr1.2 | exon | 5664737 | 5664940 | 5664737 | ID=MS.gene24527.t1.exon4;Parent=MS.gene24527.t1 |
| chr1.2 | CDS | 5664737 | 5664940 | 5664737 | ID=cds.MS.gene24527.t1;Parent=MS.gene24527.t1 |
| Gene Sequence |
| Protein sequence |
