AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24822


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAAATGAAAAGCAAATA+TGG 0.232910 8.1:-6884758 MS.gene24822:CDS
TTGCAAGAAAGAGGAGTAGA+AGG 0.398153 8.1:-6884853 MS.gene24822:CDS
TGAAAAGCAAATATGGGCAG+TGG 0.445200 8.1:-6884751 MS.gene24822:CDS
AGAAAATGAAAAGCAAATAT+GGG 0.492982 8.1:-6884757 MS.gene24822:CDS
GATTGACGAAATTAAAGATA+AGG 0.504450 8.1:-6884789 MS.gene24822:CDS
AGAACTTGAAGCTGAGAAAG+AGG 0.510711 8.1:-6884879 MS.gene24822:CDS
AAGAGGCTATTGCAAGAAAG+AGG 0.537999 8.1:-6884862 MS.gene24822:CDS
AATCTTAGCTACATTTGCAG+TGG 0.558481 8.1:+6884808 None:intergenic
GAAAAGCAAATATGGGCAGT+GGG 0.567953 8.1:-6884750 MS.gene24822:CDS
AAGGATTTCTAAAGTGCAAG+AGG 0.585321 8.1:-6884834 MS.gene24822:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATGAAAAGCAAATAT+GGG - chr8.1:6884856-6884875 MS.gene24822:CDS 20.0%
! GAGAAAATGAAAAGCAAATA+TGG - chr8.1:6884855-6884874 MS.gene24822:CDS 25.0%
! GATTGACGAAATTAAAGATA+AGG - chr8.1:6884824-6884843 MS.gene24822:CDS 25.0%
AATCTTAGCTACATTTGCAG+TGG + chr8.1:6884808-6884827 None:intergenic 35.0%
!! AAGGATTTCTAAAGTGCAAG+AGG - chr8.1:6884779-6884798 MS.gene24822:CDS 35.0%
AAGAGGCTATTGCAAGAAAG+AGG - chr8.1:6884751-6884770 MS.gene24822:CDS 40.0%
AGAACTTGAAGCTGAGAAAG+AGG - chr8.1:6884734-6884753 MS.gene24822:CDS 40.0%
GAAAAGCAAATATGGGCAGT+GGG - chr8.1:6884863-6884882 MS.gene24822:CDS 40.0%
TGAAAAGCAAATATGGGCAG+TGG - chr8.1:6884862-6884881 MS.gene24822:CDS 40.0%
TTGCAAGAAAGAGGAGTAGA+AGG - chr8.1:6884760-6884779 MS.gene24822:CDS 40.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 6884727 6884908 6884727 ID=MS.gene24822
chr8.1 mRNA 6884727 6884908 6884727 ID=MS.gene24822.t1;Parent=MS.gene24822
chr8.1 exon 6884727 6884908 6884727 ID=MS.gene24822.t1.exon1;Parent=MS.gene24822.t1
chr8.1 CDS 6884727 6884908 6884727 ID=cds.MS.gene24822.t1;Parent=MS.gene24822.t1
Gene Sequence

>MS.gene24822

ATTATGAAGAACTTGAAGCTGAGAAAGAGGCTATTGCAAGAAAGAGGAGTAGAAGGATTTCTAAAGTGCAAGAGGAATCCACTGCAAATGTAGCTAAGATTGACGAAATTAAAGATAAGGAGTTTGATGAGAAAATGAAAAGCAAATATGGGCAGTGGGTAAAAACTGATTTTGAACCATGA

Protein sequence

>MS.gene24822.t1

IMKNLKLRKRLLQERGVEGFLKCKRNPLQMULRLTKLKIRSLMRKUKANMGSGUKLILNH