Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049328.t1 | PNX71555.1 | 96.9 | 193 | 3 | 1 | 1 | 190 | 1 | 193 | 1.40E-92 | 349 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049328.t1 | A0A2K3KZ39 | 96.9 | 193 | 3 | 1 | 1 | 190 | 1 | 193 | 1.0e-92 | 349.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049328 | MS.gene049418 | 0.814552 | 1.45E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene050310 | 0.832672 | 8.27E-56 | -1.69E-46 |
| MS.gene049328 | MS.gene051067 | 0.807888 | 4.06E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene051263 | 0.80633 | 8.67E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene052731 | 0.811483 | 6.85E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene052732 | 0.837031 | 6.61E-57 | -1.69E-46 |
| MS.gene049328 | MS.gene053144 | 0.818753 | 1.66E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene054550 | 0.80909 | 2.25E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene054584 | 0.824145 | 9.46E-54 | -1.69E-46 |
| MS.gene049328 | MS.gene055537 | 0.81147 | 6.89E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene055890 | 0.81119 | 7.92E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene055891 | 0.813279 | 2.77E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene061805 | 0.826574 | 2.52E-54 | -1.69E-46 |
| MS.gene049328 | MS.gene06258 | 0.827151 | 1.83E-54 | -1.69E-46 |
| MS.gene049328 | MS.gene063452 | 0.823832 | 1.12E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene064080 | 0.802992 | 4.31E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene06448 | 0.914859 | 1.17E-84 | -1.69E-46 |
| MS.gene049328 | MS.gene065643 | 0.817361 | 3.43E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene066010 | 0.807363 | 5.24E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene06793 | 0.803152 | 3.99E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene070339 | 0.801103 | 1.05E-48 | -1.69E-46 |
| MS.gene049328 | MS.gene070885 | 0.800364 | 1.49E-48 | -1.69E-46 |
| MS.gene049328 | MS.gene071577 | 0.803442 | 3.48E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene071652 | 0.820058 | 8.38E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene07369 | 0.805376 | 1.38E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene08581 | 0.825626 | 4.23E-54 | -1.69E-46 |
| MS.gene049328 | MS.gene09275 | 0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene09575 | 0.808882 | 2.49E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene09947 | 0.830754 | 2.46E-55 | -1.69E-46 |
| MS.gene049328 | MS.gene21014 | 0.818559 | 1.84E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene21133 | 0.811344 | 7.34E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene21144 | 0.843104 | 1.72E-58 | -1.69E-46 |
| MS.gene049328 | MS.gene23880 | 0.801101 | 1.06E-48 | -1.69E-46 |
| MS.gene049328 | MS.gene24699 | 0.809539 | 1.80E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene24822 | 0.803129 | 4.04E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene25435 | 0.801774 | 7.68E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene25436 | 0.801974 | 6.99E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene25907 | 0.804458 | 2.14E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene25945 | 0.835368 | 1.75E-56 | -1.69E-46 |
| MS.gene049328 | MS.gene26224 | 0.80403 | 2.63E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene26387 | 0.812633 | 3.84E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene26855 | 0.818185 | 2.24E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene28050 | 0.817765 | 2.78E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene31006 | 0.940254 | 3.19E-100 | -1.69E-46 |
| MS.gene049328 | MS.gene31203 | 0.846137 | 2.62E-59 | -1.69E-46 |
| MS.gene049328 | MS.gene31204 | 0.837268 | 5.75E-57 | -1.69E-46 |
| MS.gene049328 | MS.gene32920 | 0.806345 | 8.61E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene33123 | 0.827077 | 1.91E-54 | -1.69E-46 |
| MS.gene049328 | MS.gene34286 | 0.821225 | 4.52E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene34590 | 0.822105 | 2.83E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene38303 | 0.82666 | 2.40E-54 | -1.69E-46 |
| MS.gene049328 | MS.gene38688 | 0.815964 | 7.06E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene38693 | 0.806856 | 6.71E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene38829 | 0.81201 | 5.26E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene39523 | 0.829968 | 3.83E-55 | -1.69E-46 |
| MS.gene049328 | MS.gene40409 | 0.822461 | 2.34E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene41164 | 0.804265 | 2.35E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene41651 | 0.819292 | 1.25E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene42510 | 0.802843 | 4.63E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene42512 | 0.812225 | 4.72E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene43171 | 0.818155 | 2.27E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene43837 | 0.811978 | 5.34E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene45479 | 0.819757 | 9.82E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene49359 | 0.819172 | 1.34E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene51091 | 0.807765 | 4.31E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene51589 | 0.824087 | 9.76E-54 | -1.69E-46 |
| MS.gene049328 | MS.gene55719 | 0.819132 | 1.36E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene57097 | 0.813385 | 2.63E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene57320 | 0.817205 | 3.72E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene57666 | 0.801898 | 7.25E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene57701 | 0.801004 | 1.10E-48 | -1.69E-46 |
| MS.gene049328 | MS.gene58772 | 0.807036 | 6.15E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene58836 | 0.815986 | 6.98E-52 | -1.69E-46 |
| MS.gene049328 | MS.gene60118 | 0.829426 | 5.18E-55 | -1.69E-46 |
| MS.gene049328 | MS.gene62916 | 0.803615 | 3.20E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene63482 | 0.820961 | 5.20E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene63853 | 0.845318 | 4.38E-59 | -1.69E-46 |
| MS.gene049328 | MS.gene65276 | 0.81206 | 5.13E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene65430 | 0.807111 | 5.93E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene68418 | 0.809975 | 1.45E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene72135 | 0.820724 | 5.90E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene72136 | 0.822691 | 2.07E-53 | -1.69E-46 |
| MS.gene049328 | MS.gene75387 | 0.812987 | 3.21E-51 | -1.69E-46 |
| MS.gene049328 | MS.gene77734 | 0.830138 | 3.48E-55 | -1.69E-46 |
| MS.gene049328 | MS.gene81409 | 0.828672 | 7.89E-55 | -1.69E-46 |
| MS.gene049328 | MS.gene82546 | 0.80294 | 4.42E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene84006 | 0.800144 | 1.65E-48 | -1.69E-46 |
| MS.gene049328 | MS.gene89081 | 0.806266 | 8.94E-50 | -1.69E-46 |
| MS.gene049328 | MS.gene99093 | 0.84142 | 4.80E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049328.t1 | MTR_3g095480 | 97.396 | 192 | 3 | 1 | 1 | 190 | 1 | 192 | 7.31e-139 | 385 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049328.t1 | AT3G18800 | 66.667 | 198 | 57 | 3 | 1 | 190 | 1 | 197 | 3.56e-89 | 260 |
Find 35 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAATTGGGCTCTTCATTT+TGG | 0.160115 | 3.1:-76168206 | MS.gene049328:CDS |
| GAACCTCCTGAGAGTTATTT+TGG | 0.184497 | 3.1:-76168239 | MS.gene049328:CDS |
| CAGACTCTTAGATGTATTAA+TGG | 0.240810 | 3.1:-76168398 | MS.gene049328:CDS |
| TTGGTAAGTTTAAGAGAAAT+TGG | 0.241730 | 3.1:-76168220 | MS.gene049328:CDS |
| AGAGTTTGAGATTCATTATC+AGG | 0.276876 | 3.1:-76168261 | MS.gene049328:CDS |
| GGGTTCTTGATCTTGATCTA+TGG | 0.279818 | 3.1:-76168185 | MS.gene049328:CDS |
| GTCTTCAATGTCGCCATTGT+TGG | 0.363130 | 3.1:-76168475 | MS.gene049328:CDS |
| TGAGGAAGATGAAGAAGATT+TGG | 0.365817 | 3.1:+76168531 | None:intergenic |
| GTTTGGCTACTGAGAAGCTT+AGG | 0.366128 | 3.1:-76168337 | MS.gene049328:CDS |
| TCATAAGCTTACACTTGGTT+TGG | 0.408278 | 3.1:-76168354 | MS.gene049328:CDS |
| TGGTAAGTTTAAGAGAAATT+GGG | 0.438263 | 3.1:-76168219 | MS.gene049328:CDS |
| GTTGTTGTTGTTGATGGTTG+TGG | 0.489653 | 3.1:+76168675 | None:intergenic |
| TTTGGCTACTGAGAAGCTTA+GGG | 0.491256 | 3.1:-76168336 | MS.gene049328:CDS |
| GGTGGTGATTATGATACTAT+TGG | 0.492628 | 3.1:-76168290 | MS.gene049328:CDS |
| CTATGGCACGTTTCTTCTCG+AGG | 0.504585 | 3.1:+76168585 | None:intergenic |
| CCCAATCACACTTCTCTCCA+TGG | 0.514078 | 3.1:-76168450 | MS.gene049328:CDS |
| GGATGATGGTGTTCAATGCT+TGG | 0.519310 | 3.1:+76168627 | None:intergenic |
| GCCATGGAGAGAAGTGTGAT+TGG | 0.521665 | 3.1:+76168449 | None:intergenic |
| CTCGAAATGGAGCTGGATGA+TGG | 0.524313 | 3.1:+76168613 | None:intergenic |
| TATGGCACGTTTCTTCTCGA+GGG | 0.531684 | 3.1:+76168586 | None:intergenic |
| AACTTACCAAAATAACTCTC+AGG | 0.534886 | 3.1:+76168233 | None:intergenic |
| TGCCATAGACCTTCAAAAGA+AGG | 0.542520 | 3.1:-76168570 | MS.gene049328:CDS |
| AGCTTAGGGATATGAAGATG+AGG | 0.544927 | 3.1:-76168322 | MS.gene049328:CDS |
| AAGATGAGGTTGAGTGGTGG+TGG | 0.558791 | 3.1:-76168308 | MS.gene049328:CDS |
| CCATGGAGAGAAGTGTGATT+GGG | 0.569183 | 3.1:+76168450 | None:intergenic |
| AGGTGTCATAAGCTTACACT+TGG | 0.582162 | 3.1:-76168359 | MS.gene049328:CDS |
| GTTGTTGTTGATGGTTGTGG+TGG | 0.583547 | 3.1:+76168678 | None:intergenic |
| CAATGGCGACATTGAAGACG+TGG | 0.610640 | 3.1:+76168479 | None:intergenic |
| ATAGAAGATGAGATGAGCCA+TGG | 0.626380 | 3.1:+76168433 | None:intergenic |
| ATGGATTCAAGTATCAGAGA+AGG | 0.640872 | 3.1:-76168379 | MS.gene049328:CDS |
| GTGTGATTGGGATCCAACAA+TGG | 0.648346 | 3.1:+76168462 | None:intergenic |
| TTACCAAAATAACTCTCAGG+AGG | 0.660707 | 3.1:+76168236 | None:intergenic |
| GTGCCATGAAAACTAAGCTG+AGG | 0.663846 | 3.1:+76168513 | None:intergenic |
| GATATGAAGATGAGGTTGAG+TGG | 0.697448 | 3.1:-76168314 | MS.gene049328:CDS |
| ATGAAGATGAGGTTGAGTGG+TGG | 0.722579 | 3.1:-76168311 | MS.gene049328:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGGTAAGTTTAAGAGAAATT+GGG | - | chr3.1:76168621-76168640 | MS.gene049328:CDS | 25.0% |
| ! | TTGGTAAGTTTAAGAGAAAT+TGG | - | chr3.1:76168620-76168639 | MS.gene049328:CDS | 25.0% |
| AACTTACCAAAATAACTCTC+AGG | + | chr3.1:76168610-76168629 | None:intergenic | 30.0% | |
| CAGACTCTTAGATGTATTAA+TGG | - | chr3.1:76168442-76168461 | MS.gene049328:CDS | 30.0% | |
| ! | AGAGTTTGAGATTCATTATC+AGG | - | chr3.1:76168579-76168598 | MS.gene049328:CDS | 30.0% |
| ATGGATTCAAGTATCAGAGA+AGG | - | chr3.1:76168461-76168480 | MS.gene049328:CDS | 35.0% | |
| GGTGGTGATTATGATACTAT+TGG | - | chr3.1:76168550-76168569 | MS.gene049328:CDS | 35.0% | |
| TCATAAGCTTACACTTGGTT+TGG | - | chr3.1:76168486-76168505 | MS.gene049328:CDS | 35.0% | |
| TGAGGAAGATGAAGAAGATT+TGG | + | chr3.1:76168312-76168331 | None:intergenic | 35.0% | |
| TTACCAAAATAACTCTCAGG+AGG | + | chr3.1:76168607-76168626 | None:intergenic | 35.0% | |
| ! | AGAAATTGGGCTCTTCATTT+TGG | - | chr3.1:76168634-76168653 | MS.gene049328:CDS | 35.0% |
| ! | GAAATTGGGCTCTTCATTTT+GGG | - | chr3.1:76168635-76168654 | MS.gene049328:CDS | 35.0% |
| ! | TGTGAAGTTCCTTCTTTTGA+AGG | + | chr3.1:76168282-76168301 | None:intergenic | 35.0% |
| !! | CTACTTGTTGTTGTTGTTGA+TGG | + | chr3.1:76168174-76168193 | None:intergenic | 35.0% |
| !! | TTCCTTCTTTTGAAGGTCTA+TGG | + | chr3.1:76168275-76168294 | None:intergenic | 35.0% |
| AGCTTAGGGATATGAAGATG+AGG | - | chr3.1:76168518-76168537 | MS.gene049328:CDS | 40.0% | |
| ATAGAAGATGAGATGAGCCA+TGG | + | chr3.1:76168410-76168429 | None:intergenic | 40.0% | |
| GATATGAAGATGAGGTTGAG+TGG | - | chr3.1:76168526-76168545 | MS.gene049328:CDS | 40.0% | |
| GGGTTCTTGATCTTGATCTA+TGG | - | chr3.1:76168655-76168674 | MS.gene049328:CDS | 40.0% | |
| TGCCATAGACCTTCAAAAGA+AGG | - | chr3.1:76168270-76168289 | MS.gene049328:CDS | 40.0% | |
| TTTGGCTACTGAGAAGCTTA+GGG | - | chr3.1:76168504-76168523 | MS.gene049328:CDS | 40.0% | |
| ! | AGGTGTCATAAGCTTACACT+TGG | - | chr3.1:76168481-76168500 | MS.gene049328:CDS | 40.0% |
| ! | GAACCTCCTGAGAGTTATTT+TGG | - | chr3.1:76168601-76168620 | MS.gene049328:CDS | 40.0% |
| !! | CTTCCTCAGCTTAGTTTTCA+TGG | - | chr3.1:76168324-76168343 | MS.gene049328:CDS | 40.0% |
| !! | GTTGTTGTTGTTGATGGTTG+TGG | + | chr3.1:76168168-76168187 | None:intergenic | 40.0% |
| ATGAAGATGAGGTTGAGTGG+TGG | - | chr3.1:76168529-76168548 | MS.gene049328:CDS | 45.0% | |
| GTCTTCAATGTCGCCATTGT+TGG | - | chr3.1:76168365-76168384 | MS.gene049328:CDS | 45.0% | |
| GTGCCATGAAAACTAAGCTG+AGG | + | chr3.1:76168330-76168349 | None:intergenic | 45.0% | |
| GTGTGATTGGGATCCAACAA+TGG | + | chr3.1:76168381-76168400 | None:intergenic | 45.0% | |
| GTTTGGCTACTGAGAAGCTT+AGG | - | chr3.1:76168503-76168522 | MS.gene049328:CDS | 45.0% | |
| TATGGCACGTTTCTTCTCGA+GGG | + | chr3.1:76168257-76168276 | None:intergenic | 45.0% | |
| ! | CCATGGAGAGAAGTGTGATT+GGG | + | chr3.1:76168393-76168412 | None:intergenic | 45.0% |
| ! | GGATGATGGTGTTCAATGCT+TGG | + | chr3.1:76168216-76168235 | None:intergenic | 45.0% |
| ! | TCTCGAGGGTTTTCTCGAAA+TGG | + | chr3.1:76168243-76168262 | None:intergenic | 45.0% |
| !! | GTTGTTGTTGATGGTTGTGG+TGG | + | chr3.1:76168165-76168184 | None:intergenic | 45.0% |
| AAGATGAGGTTGAGTGGTGG+TGG | - | chr3.1:76168532-76168551 | MS.gene049328:CDS | 50.0% | |
| CAATGGCGACATTGAAGACG+TGG | + | chr3.1:76168364-76168383 | None:intergenic | 50.0% | |
| CCCAATCACACTTCTCTCCA+TGG | - | chr3.1:76168390-76168409 | MS.gene049328:CDS | 50.0% | |
| CTATGGCACGTTTCTTCTCG+AGG | + | chr3.1:76168258-76168277 | None:intergenic | 50.0% | |
| CTCGAAATGGAGCTGGATGA+TGG | + | chr3.1:76168230-76168249 | None:intergenic | 50.0% | |
| ! | GCCATGGAGAGAAGTGTGAT+TGG | + | chr3.1:76168394-76168413 | None:intergenic | 50.0% |
| ! | GGGTTTTCTCGAAATGGAGC+TGG | + | chr3.1:76168237-76168256 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 76168145 | 76168717 | 76168145 | ID=MS.gene049328 |
| chr3.1 | mRNA | 76168145 | 76168717 | 76168145 | ID=MS.gene049328.t1;Parent=MS.gene049328 |
| chr3.1 | exon | 76168145 | 76168717 | 76168145 | ID=MS.gene049328.t1.exon1;Parent=MS.gene049328.t1 |
| chr3.1 | CDS | 76168145 | 76168717 | 76168145 | ID=cds.MS.gene049328.t1;Parent=MS.gene049328.t1 |
| Gene Sequence |
| Protein sequence |
