Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25114.t1 | RHN39479.1 | 65.8 | 453 | 22 | 1 | 83 | 402 | 9 | 461 | 4.70E-154 | 554.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25114.t1 | P38414 | 46.3 | 575 | 54 | 1 | 83 | 402 | 292 | 866 | 4.5e-129 | 462.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25114.t1 | A0A396GDH7 | 65.8 | 453 | 22 | 1 | 83 | 402 | 9 | 461 | 3.4e-154 | 554.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050800 | MS.gene25114 | 0.830272 | 3.22E-55 | -1.69E-46 |
| MS.gene051113 | MS.gene25114 | 0.916297 | 2.11E-85 | -1.69E-46 |
| MS.gene051255 | MS.gene25114 | -0.802414 | 5.68E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene25114 | -0.826456 | 2.69E-54 | -1.69E-46 |
| MS.gene052379 | MS.gene25114 | -0.804252 | 2.36E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene25114 | -0.814445 | 1.53E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene25114 | -0.802322 | 5.93E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene25114 | -0.808459 | 3.06E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene25114 | -0.806508 | 7.95E-50 | -1.69E-46 |
| MS.gene055941 | MS.gene25114 | -0.805089 | 1.58E-49 | -1.69E-46 |
| MS.gene056410 | MS.gene25114 | -0.808725 | 2.69E-50 | -1.69E-46 |
| MS.gene056448 | MS.gene25114 | -0.824546 | 7.61E-54 | -1.69E-46 |
| MS.gene056923 | MS.gene25114 | 0.805712 | 1.17E-49 | -1.69E-46 |
| MS.gene056942 | MS.gene25114 | -0.823579 | 1.28E-53 | -1.69E-46 |
| MS.gene057845 | MS.gene25114 | -0.806047 | 9.95E-50 | -1.69E-46 |
| MS.gene058323 | MS.gene25114 | -0.802099 | 6.59E-49 | -1.69E-46 |
| MS.gene058840 | MS.gene25114 | 0.808777 | 2.62E-50 | -1.69E-46 |
| MS.gene06074 | MS.gene25114 | 0.82991 | 3.95E-55 | -1.69E-46 |
| MS.gene060804 | MS.gene25114 | -0.826537 | 2.57E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25114.t1 | MTR_8g021020 | 93.822 | 259 | 16 | 0 | 144 | 402 | 173 | 431 | 5.39e-180 | 508 |
| MS.gene25114.t1 | MTR_8g021020 | 87.179 | 39 | 5 | 0 | 84 | 122 | 1 | 39 | 2.34e-14 | 74.7 |
| MS.gene25114.t1 | MTR_8g020990 | 95.367 | 259 | 12 | 0 | 144 | 402 | 610 | 868 | 1.92e-177 | 517 |
| MS.gene25114.t1 | MTR_8g020990 | 100.000 | 69 | 0 | 0 | 83 | 151 | 294 | 362 | 2.34e-37 | 144 |
| MS.gene25114.t1 | MTR_8g018570 | 84.170 | 259 | 41 | 0 | 144 | 402 | 612 | 870 | 2.71e-158 | 468 |
| MS.gene25114.t1 | MTR_8g018570 | 79.710 | 69 | 14 | 0 | 83 | 151 | 296 | 364 | 2.97e-30 | 123 |
| MS.gene25114.t1 | MTR_8g018550 | 83.784 | 259 | 42 | 0 | 144 | 402 | 610 | 868 | 8.57e-155 | 459 |
| MS.gene25114.t1 | MTR_8g018550 | 85.507 | 69 | 10 | 0 | 83 | 151 | 294 | 362 | 5.93e-32 | 128 |
| MS.gene25114.t1 | MTR_8g018690 | 83.333 | 252 | 42 | 0 | 151 | 402 | 594 | 845 | 3.24e-151 | 449 |
| MS.gene25114.t1 | MTR_8g018690 | 65.217 | 69 | 19 | 1 | 83 | 151 | 276 | 339 | 5.67e-21 | 95.9 |
| MS.gene25114.t1 | MTR_8g018690 | 83.333 | 252 | 42 | 0 | 151 | 402 | 614 | 865 | 5.29e-151 | 449 |
| MS.gene25114.t1 | MTR_8g018690 | 65.217 | 69 | 19 | 1 | 83 | 151 | 296 | 359 | 6.34e-21 | 95.5 |
| MS.gene25114.t1 | MTR_8g018430 | 81.349 | 252 | 47 | 0 | 151 | 402 | 610 | 861 | 1.38e-149 | 445 |
| MS.gene25114.t1 | MTR_8g018430 | 72.464 | 69 | 19 | 0 | 83 | 151 | 287 | 355 | 3.01e-27 | 114 |
| MS.gene25114.t1 | MTR_8g018450 | 80.952 | 252 | 48 | 0 | 151 | 402 | 577 | 828 | 1.80e-148 | 441 |
| MS.gene25114.t1 | MTR_8g018510 | 79.762 | 252 | 51 | 0 | 151 | 402 | 618 | 869 | 2.53e-146 | 437 |
| MS.gene25114.t1 | MTR_8g018510 | 75.000 | 68 | 17 | 0 | 84 | 151 | 296 | 363 | 3.26e-28 | 117 |
| MS.gene25114.t1 | MTR_8g018520 | 77.606 | 259 | 58 | 0 | 144 | 402 | 610 | 868 | 3.59e-145 | 434 |
| MS.gene25114.t1 | MTR_8g018520 | 73.913 | 69 | 18 | 0 | 83 | 151 | 294 | 362 | 2.69e-26 | 111 |
| MS.gene25114.t1 | MTR_8g018650 | 74.903 | 259 | 65 | 0 | 144 | 402 | 549 | 807 | 6.89e-141 | 421 |
| MS.gene25114.t1 | MTR_8g018620 | 75.290 | 259 | 63 | 1 | 144 | 402 | 550 | 807 | 5.93e-139 | 416 |
| MS.gene25114.t1 | MTR_8g018590 | 72.619 | 252 | 68 | 1 | 151 | 402 | 573 | 823 | 4.93e-126 | 383 |
| MS.gene25114.t1 | MTR_2g099570 | 67.181 | 259 | 84 | 1 | 144 | 402 | 604 | 861 | 9.68e-120 | 368 |
| MS.gene25114.t1 | MTR_2g099570 | 58.571 | 70 | 28 | 1 | 83 | 151 | 287 | 356 | 1.32e-18 | 88.6 |
| MS.gene25114.t1 | MTR_8g018420 | 64.479 | 259 | 92 | 0 | 144 | 402 | 598 | 856 | 3.86e-117 | 361 |
| MS.gene25114.t1 | MTR_8g018420 | 57.971 | 69 | 29 | 0 | 83 | 151 | 282 | 350 | 8.51e-19 | 89.0 |
| MS.gene25114.t1 | MTR_8g018730 | 62.162 | 259 | 97 | 1 | 144 | 402 | 327 | 584 | 6.70e-115 | 347 |
| MS.gene25114.t1 | MTR_8g018730 | 55.714 | 70 | 30 | 1 | 83 | 151 | 11 | 80 | 8.83e-18 | 85.5 |
| MS.gene25114.t1 | MTR_2g099560 | 63.707 | 259 | 93 | 1 | 144 | 402 | 606 | 863 | 3.56e-114 | 353 |
| MS.gene25114.t1 | MTR_2g099560 | 57.971 | 69 | 29 | 0 | 83 | 151 | 292 | 360 | 2.25e-18 | 87.8 |
| MS.gene25114.t1 | MTR_3g081130 | 60.618 | 259 | 101 | 1 | 144 | 402 | 529 | 786 | 3.37e-112 | 346 |
| MS.gene25114.t1 | MTR_3g081130 | 52.857 | 70 | 30 | 1 | 83 | 152 | 215 | 281 | 7.49e-17 | 83.2 |
| MS.gene25114.t1 | MTR_5g024020 | 62.548 | 259 | 96 | 1 | 144 | 402 | 574 | 831 | 3.48e-112 | 347 |
| MS.gene25114.t1 | MTR_5g024020 | 62.264 | 53 | 20 | 0 | 99 | 151 | 311 | 363 | 9.83e-17 | 82.8 |
| MS.gene25114.t1 | MTR_5g024020 | 62.548 | 259 | 96 | 1 | 144 | 402 | 610 | 867 | 6.02e-112 | 348 |
| MS.gene25114.t1 | MTR_5g024020 | 62.264 | 53 | 20 | 0 | 99 | 151 | 311 | 363 | 9.76e-17 | 82.8 |
| MS.gene25114.t1 | MTR_3g081130 | 60.618 | 259 | 101 | 1 | 144 | 402 | 601 | 858 | 8.07e-112 | 347 |
| MS.gene25114.t1 | MTR_3g081130 | 52.857 | 70 | 30 | 1 | 83 | 152 | 287 | 353 | 7.34e-17 | 83.2 |
| MS.gene25114.t1 | MTR_8g018730 | 62.162 | 259 | 97 | 1 | 144 | 402 | 577 | 834 | 8.71e-112 | 346 |
| MS.gene25114.t1 | MTR_8g018730 | 43.434 | 99 | 50 | 2 | 55 | 151 | 236 | 330 | 5.05e-17 | 83.6 |
| MS.gene25114.t1 | MTR_8g018730 | 62.162 | 259 | 97 | 1 | 144 | 402 | 607 | 864 | 1.25e-111 | 347 |
| MS.gene25114.t1 | MTR_8g018730 | 55.714 | 70 | 30 | 1 | 83 | 151 | 291 | 360 | 1.31e-17 | 85.5 |
| MS.gene25114.t1 | MTR_8g018735 | 62.162 | 259 | 97 | 1 | 144 | 402 | 608 | 865 | 1.72e-110 | 344 |
| MS.gene25114.t1 | MTR_8g018735 | 58.571 | 70 | 28 | 1 | 83 | 151 | 292 | 361 | 1.01e-18 | 89.0 |
| MS.gene25114.t1 | MTR_7g113410 | 60.232 | 259 | 102 | 1 | 144 | 402 | 610 | 867 | 1.81e-107 | 336 |
| MS.gene25114.t1 | MTR_1g104650 | 55.642 | 257 | 108 | 2 | 151 | 402 | 601 | 856 | 1.88e-100 | 317 |
| MS.gene25114.t1 | MTR_1g083020 | 59.073 | 259 | 105 | 1 | 144 | 402 | 601 | 858 | 6.36e-100 | 316 |
| MS.gene25114.t1 | MTR_8g020970 | 44.118 | 272 | 149 | 1 | 130 | 401 | 658 | 926 | 7.17e-78 | 258 |
| MS.gene25114.t1 | MTR_4g066170 | 44.656 | 262 | 141 | 3 | 144 | 402 | 646 | 906 | 2.32e-70 | 238 |
| MS.gene25114.t1 | MTR_8g080230 | 40.511 | 274 | 159 | 2 | 130 | 402 | 646 | 916 | 8.70e-70 | 236 |
| MS.gene25114.t1 | MTR_4g088810 | 39.796 | 294 | 170 | 3 | 111 | 401 | 605 | 894 | 7.06e-66 | 225 |
| MS.gene25114.t1 | MTR_3g079450 | 41.091 | 275 | 156 | 3 | 130 | 402 | 632 | 902 | 1.12e-65 | 225 |
| MS.gene25114.t1 | MTR_3g479460 | 43.307 | 254 | 141 | 2 | 151 | 402 | 651 | 903 | 2.35e-65 | 224 |
| MS.gene25114.t1 | MTR_6g452500 | 36.634 | 303 | 172 | 7 | 97 | 385 | 309 | 605 | 1.72e-57 | 198 |
| MS.gene25114.t1 | MTR_3g079420 | 42.146 | 261 | 148 | 2 | 144 | 402 | 606 | 865 | 1.09e-53 | 191 |
| MS.gene25114.t1 | MTR_8g011340 | 40.191 | 209 | 124 | 1 | 156 | 363 | 620 | 828 | 1.04e-48 | 177 |
| MS.gene25114.t1 | MTR_8g021025 | 98.387 | 62 | 1 | 0 | 21 | 82 | 68 | 129 | 3.09e-36 | 129 |
| MS.gene25114.t1 | MTR_8g018735 | 58.571 | 70 | 28 | 1 | 83 | 151 | 292 | 361 | 9.28e-19 | 89.0 |
| MS.gene25114.t1 | MTR_4g088805 | 38.462 | 91 | 56 | 0 | 311 | 401 | 1 | 91 | 2.31e-16 | 74.3 |
| MS.gene25114.t1 | MTR_7g113408 | 42.857 | 63 | 35 | 1 | 294 | 356 | 8 | 69 | 4.27e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25114.t1 | AT3G22400 | 56.061 | 264 | 110 | 2 | 144 | 402 | 624 | 886 | 5.42e-100 | 317 |
| MS.gene25114.t1 | AT1G55020 | 56.746 | 252 | 108 | 1 | 151 | 402 | 609 | 859 | 2.26e-99 | 315 |
| MS.gene25114.t1 | AT1G72520 | 42.857 | 273 | 153 | 1 | 130 | 402 | 657 | 926 | 6.22e-75 | 251 |
| MS.gene25114.t1 | AT1G17420 | 42.857 | 273 | 153 | 1 | 130 | 402 | 650 | 919 | 7.08e-73 | 245 |
| MS.gene25114.t1 | AT1G67560 | 41.606 | 274 | 156 | 2 | 130 | 402 | 647 | 917 | 2.63e-66 | 227 |
| MS.gene25114.t1 | AT3G45140 | 39.928 | 278 | 157 | 4 | 129 | 402 | 483 | 754 | 1.61e-61 | 211 |
| MS.gene25114.t1 | AT3G45140 | 39.928 | 278 | 157 | 4 | 129 | 402 | 625 | 896 | 6.51e-61 | 212 |
Find 93 sgRNAs with CRISPR-Local
Find 271 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATGCAGCTGTTAATTT+TGG | 0.137230 | 8.4:+72622611 | MS.gene25114:CDS |
| ATTCTGAACTCCAAGCATTT+TGG | 0.213611 | 8.4:+72622471 | MS.gene25114:CDS |
| AACCTTCCGCCGTCTGAATA+TGG | 0.240508 | 8.4:-72618944 | None:intergenic |
| AACTTCTTGTAGGCCTGTTT+TGG | 0.254785 | 8.4:-72622881 | None:intergenic |
| CAAAAGTGCTTTCCATAAAA+AGG | 0.284199 | 8.4:-72619161 | None:intergenic |
| TATATACCTTGAACCACTTT+AGG | 0.295558 | 8.4:-72621512 | None:intergenic |
| CTTCTTTCCAAACTTCTTGT+AGG | 0.296818 | 8.4:-72622891 | None:intergenic |
| CTTCAGATGGACTAGAGATA+TGG | 0.321694 | 8.4:+72622378 | MS.gene25114:CDS |
| GTTCTTTGAACAACGGTATT+GGG | 0.323229 | 8.4:-72621448 | None:intergenic |
| AGTTCTTTGAACAACGGTAT+TGG | 0.344989 | 8.4:-72621449 | None:intergenic |
| ACAACGGTATTGGGCTAATC+TGG | 0.354078 | 8.4:-72621439 | None:intergenic |
| TCTACTTATCCTTCTCGGTT+TGG | 0.359316 | 8.4:+72619207 | MS.gene25114:CDS |
| GTAGGATAAAGCAGAGTATA+TGG | 0.362111 | 8.4:-72622992 | None:intergenic |
| AAACAGGCCTACAAGAAGTT+TGG | 0.372935 | 8.4:+72622884 | MS.gene25114:CDS |
| ATTTAGAGGAGTGGCAGTTC+AGG | 0.377653 | 8.4:+72622304 | MS.gene25114:intron |
| AGGTGGGTCATGGTGACTTT+AGG | 0.378805 | 8.4:+72622507 | MS.gene25114:CDS |
| AGAGAATCGAAGGCGATCTC+TGG | 0.382540 | 8.4:+72622843 | MS.gene25114:CDS |
| ACTTATCTACTTATCCTTCT+CGG | 0.384698 | 8.4:+72619202 | MS.gene25114:CDS |
| CCTTGACAAGACCTCTAAAA+TGG | 0.394691 | 8.4:-72622792 | None:intergenic |
| TTCTGATTCACAGCCAAAAC+AGG | 0.396436 | 8.4:+72622868 | MS.gene25114:CDS |
| CAAGTTCTTTCCAAAATGCT+TGG | 0.406902 | 8.4:-72622481 | None:intergenic |
| TTGTCCTCAAATAAGTCTTC+TGG | 0.407224 | 8.4:-72622736 | None:intergenic |
| AAGCTTGCACCATTCTCATA+TGG | 0.416322 | 8.4:+72622576 | MS.gene25114:CDS |
| GTAGCTTTAGATCTCAAATT+CGG | 0.426348 | 8.4:-72621326 | None:intergenic |
| TAGGTTATTGAAACGGCTTA+AGG | 0.428816 | 8.4:+72619071 | MS.gene25114:intron |
| GACACTGTTGGGAATGCCTC+TGG | 0.441705 | 8.4:-72623035 | None:intergenic |
| AGAGTATATGGCACCTTCAC+AGG | 0.447713 | 8.4:-72622980 | None:intergenic |
| CAAGAAGTTTGGAAAGAAGT+TGG | 0.456294 | 8.4:+72622895 | MS.gene25114:CDS |
| TAAGTTTCCACCACCTAAAG+TGG | 0.469195 | 8.4:+72621499 | MS.gene25114:CDS |
| GAGACACTAAGGAATCGAAA+TGG | 0.470257 | 8.4:+72622956 | MS.gene25114:CDS |
| CCACCACCTAAAGTGGTTCA+AGG | 0.472852 | 8.4:+72621506 | MS.gene25114:CDS |
| TCTTCAAAGGTGTTAAACTC+AGG | 0.476777 | 8.4:-72621365 | None:intergenic |
| CGAACACCGTGTGGAGAAGC+TGG | 0.482213 | 8.4:-72622329 | None:intergenic |
| CTCTGCTTTATCCTACTAGT+GGG | 0.492357 | 8.4:+72622999 | MS.gene25114:CDS |
| GAATATGGAGACCTTGCGGC+GGG | 0.492635 | 8.4:-72618929 | None:intergenic |
| TCTAGTCCATCTGAAGCATA+AGG | 0.496890 | 8.4:-72622371 | None:intergenic |
| TTCAGATGGACTAGAGATAT+GGG | 0.502780 | 8.4:+72622379 | MS.gene25114:CDS |
| TACTCAACAGATCCTTTCTC+AGG | 0.503501 | 8.4:-72622692 | None:intergenic |
| AATATCTTAGGTTATTGAAA+CGG | 0.511068 | 8.4:+72619064 | MS.gene25114:intron |
| CTTGATAATTTACATGAAGA+GGG | 0.520792 | 8.4:-72619247 | None:intergenic |
| AGTAAGAGTATCATTCTTTG+CGG | 0.524801 | 8.4:-72622762 | None:intergenic |
| AGAAGATTCATGCCTGAGAA+AGG | 0.525970 | 8.4:+72622680 | MS.gene25114:CDS |
| TTGGAAAGAACTTGTAGAGG+TGG | 0.530830 | 8.4:+72622490 | MS.gene25114:CDS |
| GACTATCCTTATGCTTCAGA+TGG | 0.531990 | 8.4:+72622365 | MS.gene25114:CDS |
| CCTTGAACCACTTTAGGTGG+TGG | 0.532735 | 8.4:-72621506 | None:intergenic |
| GTGAAAGATCTTATTGAAGC+TGG | 0.539200 | 8.4:+72619111 | MS.gene25114:CDS |
| ATTTACATGAAGAGGGTTCG+AGG | 0.539387 | 8.4:-72619240 | None:intergenic |
| GTCAATCCTTCCCCACTAGT+AGG | 0.539688 | 8.4:-72623010 | None:intergenic |
| TGGAAAGAACTTGTAGAGGT+GGG | 0.541949 | 8.4:+72622491 | MS.gene25114:CDS |
| CGGTGTTCGACTTCTGATAG+AGG | 0.542094 | 8.4:+72622343 | MS.gene25114:CDS |
| ACTTGATAATTTACATGAAG+AGG | 0.542947 | 8.4:-72619248 | None:intergenic |
| CTTTATCCTACTAGTGGGGA+AGG | 0.544538 | 8.4:+72623004 | MS.gene25114:CDS |
| CTTCAAAGGTGTTAAACTCA+GGG | 0.545165 | 8.4:-72621364 | None:intergenic |
| CATCATTGTTCCTTTGAACG+AGG | 0.545950 | 8.4:-72622934 | None:intergenic |
| AACATGGCAGCCCCGCCGCA+AGG | 0.546242 | 8.4:+72618918 | None:intergenic |
| GCGTTGTTGAAAAGTTTCAA+TGG | 0.549801 | 8.4:-72619139 | None:intergenic |
| TGAAGAAATCCATATGAGAA+TGG | 0.553542 | 8.4:-72622585 | None:intergenic |
| TATGGGCTGCTATTAAGTCA+TGG | 0.554200 | 8.4:+72622396 | MS.gene25114:CDS |
| GTTTAAGATGCAAACTCGTA+CGG | 0.554784 | 8.4:+72622544 | MS.gene25114:CDS |
| CTCCATATTCAGACGGCGGA+AGG | 0.556606 | 8.4:+72618942 | MS.gene25114:CDS |
| GCTTCTGATGAACAATACCT+TGG | 0.559094 | 8.4:+72622818 | MS.gene25114:CDS |
| CGCCTTCGATTCTCTCTCCA+AGG | 0.560317 | 8.4:-72622835 | None:intergenic |
| GGAACAATGATGAGACACTA+AGG | 0.560598 | 8.4:+72622945 | MS.gene25114:CDS |
| ATGGGCTGCTATTAAGTCAT+GGG | 0.567374 | 8.4:+72622397 | MS.gene25114:CDS |
| AAGGTTCAAACGGTGAGGCT+AGG | 0.576644 | 8.4:+72618961 | MS.gene25114:CDS |
| ACTACCAGAAGACTTATTTG+AGG | 0.580355 | 8.4:+72622732 | MS.gene25114:CDS |
| GAATCGAAATGGACCTGTGA+AGG | 0.580490 | 8.4:+72622967 | MS.gene25114:CDS |
| ACTCTGCTTTATCCTACTAG+TGG | 0.581194 | 8.4:+72622998 | MS.gene25114:CDS |
| GAGAGAGCGTATATCTTCAA+AGG | 0.581939 | 8.4:-72621378 | None:intergenic |
| ATACCTTGAACCACTTTAGG+TGG | 0.590734 | 8.4:-72621509 | None:intergenic |
| GAACTTGTAGAGGTGGGTCA+TGG | 0.590953 | 8.4:+72622497 | MS.gene25114:CDS |
| TACCTTGGAGAGAGAATCGA+AGG | 0.592874 | 8.4:+72622833 | MS.gene25114:CDS |
| CAGGATCCAGCTTCTCCACA+CGG | 0.597050 | 8.4:+72622323 | MS.gene25114:CDS |
| CAGACGGCGGAAGGTTCAAA+CGG | 0.612212 | 8.4:+72618951 | MS.gene25114:CDS |
| TAATCTGAACCAAACCGAGA+AGG | 0.614730 | 8.4:-72619216 | None:intergenic |
| TTTGGACAATATGCGTATGG+AGG | 0.620897 | 8.4:+72622629 | MS.gene25114:CDS |
| ATACGCTCTCTCTACGATGG+TGG | 0.622802 | 8.4:+72621389 | MS.gene25114:CDS |
| TGAGTATGATGAGCTTGCTA+AGG | 0.630344 | 8.4:+72622709 | MS.gene25114:CDS |
| ATCTGGCTTAGTACATCAGT+AGG | 0.631513 | 8.4:-72621422 | None:intergenic |
| ATCAGAAGTCGAACACCGTG+TGG | 0.634779 | 8.4:-72622338 | None:intergenic |
| GGGGAAGGATTGACTTCCAG+AGG | 0.639868 | 8.4:+72623019 | MS.gene25114:CDS |
| TGAATATGGAGACCTTGCGG+CGG | 0.640070 | 8.4:-72618930 | None:intergenic |
| TAGATAAGTTAACACCAAGA+GGG | 0.640424 | 8.4:-72619188 | None:intergenic |
| GTAGATAAGTTAACACCAAG+AGG | 0.647293 | 8.4:-72619189 | None:intergenic |
| GCAAGGTCTCCATATTCAGA+CGG | 0.653088 | 8.4:+72618935 | MS.gene25114:CDS |
| GTCTGAATATGGAGACCTTG+CGG | 0.668375 | 8.4:-72618933 | None:intergenic |
| GGCGGAAGGTTCAAACGGTG+AGG | 0.671562 | 8.4:+72618956 | MS.gene25114:CDS |
| GATATACGCTCTCTCTACGA+TGG | 0.675831 | 8.4:+72621386 | MS.gene25114:CDS |
| ACTTTAGGAATGCAACATGG+TGG | 0.698879 | 8.4:+72622522 | MS.gene25114:CDS |
| AATATGGAGACCTTGCGGCG+GGG | 0.701594 | 8.4:-72618928 | None:intergenic |
| TCTGCTTTATCCTACTAGTG+GGG | 0.710796 | 8.4:+72623000 | MS.gene25114:CDS |
| AGGTCTCCATATTCAGACGG+CGG | 0.717616 | 8.4:+72618938 | MS.gene25114:CDS |
| GTGACTTTAGGAATGCAACA+TGG | 0.726875 | 8.4:+72622519 | MS.gene25114:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATTTTATAATAAAGAAAA+AGG | - | chr8.4:72619497-72619516 | None:intergenic | 10.0% |
| !! | CAAATAAACCAAAAAAAAAA+AGG | + | chr8.4:72621106-72621125 | MS.gene25114:intron | 15.0% |
| !! | CAATATTACAAAAACAAAAT+TGG | - | chr8.4:72622252-72622271 | None:intergenic | 15.0% |
| !! | GAAAAAATAAAAGCAATAAA+AGG | - | chr8.4:72619528-72619547 | None:intergenic | 15.0% |
| !! | GAAATGTAAAAACAAAAAAA+AGG | - | chr8.4:72619638-72619657 | None:intergenic | 15.0% |
| !! | TAAAAGTTAAAAATTGGTTA+GGG | + | chr8.4:72620165-72620184 | MS.gene25114:intron | 15.0% |
| !! | TTATGGTAAAAGTTAAAAAT+TGG | + | chr8.4:72620159-72620178 | MS.gene25114:intron | 15.0% |
| !!! | AATTTTTATCTTTCATTTCT+AGG | + | chr8.4:72619726-72619745 | MS.gene25114:intron | 15.0% |
| !!! | ACTAACTTTTATATTTCTAT+TGG | + | chr8.4:72622146-72622165 | MS.gene25114:intron | 15.0% |
| !!! | TCAATATCTTTTTAGAAAAA+TGG | - | chr8.4:72622028-72622047 | None:intergenic | 15.0% |
| !! | AAGAAAAAGGAAAGAAAATA+AGG | - | chr8.4:72619484-72619503 | None:intergenic | 20.0% |
| !! | AATATCTTAGGTTATTGAAA+CGG | + | chr8.4:72619064-72619083 | MS.gene25114:intron | 20.0% |
| !! | ACCTAAAATTAAAATCACAA+GGG | - | chr8.4:72619553-72619572 | None:intergenic | 20.0% |
| !! | AGATAAAATTTATACCGTTT+AGG | + | chr8.4:72619900-72619919 | MS.gene25114:intron | 20.0% |
| !! | AGTTTGAACAATTACAAAAA+TGG | - | chr8.4:72620569-72620588 | None:intergenic | 20.0% |
| !! | ATTAATGAAATTCATACCAA+TGG | - | chr8.4:72621632-72621651 | None:intergenic | 20.0% |
| !! | CCTAAAAATAAATTAAGTGA+GGG | + | chr8.4:72621553-72621572 | MS.gene25114:intron | 20.0% |
| !! | GAAGTAAAAGTTTATGAATA+GGG | - | chr8.4:72621797-72621816 | None:intergenic | 20.0% |
| !! | GTAAAAGTTAAAAATTGGTT+AGG | + | chr8.4:72620164-72620183 | MS.gene25114:intron | 20.0% |
| !! | TAATACCCAAAATGAATTAT+GGG | + | chr8.4:72620116-72620135 | MS.gene25114:intron | 20.0% |
| !! | TACCTAAAATTAAAATCACA+AGG | - | chr8.4:72619554-72619573 | None:intergenic | 20.0% |
| !! | TTAATACCCAAAATGAATTA+TGG | + | chr8.4:72620115-72620134 | MS.gene25114:intron | 20.0% |
| !! | TTTATTGTATTGTTATCACT+TGG | - | chr8.4:72620733-72620752 | None:intergenic | 20.0% |
| !!! | ATATTTTTGTAATGTTCATG+AGG | + | chr8.4:72622056-72622075 | MS.gene25114:intron | 20.0% |
| !!! | CCTCACTTAATTTATTTTTA+GGG | - | chr8.4:72621555-72621574 | None:intergenic | 20.0% |
| !!! | CTTAATTTTGAAATGGAATT+TGG | - | chr8.4:72621065-72621084 | None:intergenic | 20.0% |
| !!! | TAATTTTAGGTAAATTGTGA+GGG | + | chr8.4:72619562-72619581 | MS.gene25114:intron | 20.0% |
| !!! | TATCTTTTGATTAGATCATT+AGG | - | chr8.4:72621975-72621994 | None:intergenic | 20.0% |
| !!! | TATGAATGTTCTTGTTTATT+GGG | + | chr8.4:72619609-72619628 | MS.gene25114:intron | 20.0% |
| !!! | TTAATTTTAGGTAAATTGTG+AGG | + | chr8.4:72619561-72619580 | MS.gene25114:intron | 20.0% |
| !!! | TTAGTTAGAGTTTGTTATTA+GGG | - | chr8.4:72622131-72622150 | None:intergenic | 20.0% |
| ! | AATAATGTTTGGATCTGTTA+TGG | + | chr8.4:72620142-72620161 | MS.gene25114:intron | 25.0% |
| ! | AATTTATGTTCATTGTGTCA+CGG | - | chr8.4:72621827-72621846 | None:intergenic | 25.0% |
| ! | ACAAAAACAAAATTGGAGAA+GGG | - | chr8.4:72622245-72622264 | None:intergenic | 25.0% |
| ! | AGTAATTGTGTTAATTGTGT+TGG | + | chr8.4:72621163-72621182 | MS.gene25114:intron | 25.0% |
| ! | ATTATGGGAAGAATAATGTT+TGG | + | chr8.4:72620131-72620150 | MS.gene25114:intron | 25.0% |
| ! | CCCTAAAAATAAATTAAGTG+AGG | + | chr8.4:72621552-72621571 | MS.gene25114:intron | 25.0% |
| ! | CCTAAAATTAAAATCACAAG+GGG | - | chr8.4:72619552-72619571 | None:intergenic | 25.0% |
| ! | GCATAGTTAGAATGTTTAAA+AGG | + | chr8.4:72619016-72619035 | MS.gene25114:intron | 25.0% |
| ! | GGAAGTAAAAGTTTATGAAT+AGG | - | chr8.4:72621798-72621817 | None:intergenic | 25.0% |
| ! | TACAAAAACAAAATTGGAGA+AGG | - | chr8.4:72622246-72622265 | None:intergenic | 25.0% |
| ! | TATATATCTCAATGTTTGAC+AGG | + | chr8.4:72621706-72621725 | MS.gene25114:intron | 25.0% |
| ! | TATGGAATTAACTTTCTTGT+GGG | + | chr8.4:72621884-72621903 | MS.gene25114:intron | 25.0% |
| ! | TCAATGTTGAAGCTAATAAT+GGG | + | chr8.4:72620437-72620456 | MS.gene25114:intron | 25.0% |
| ! | TCTTTGTGAGAAAACAATAT+AGG | + | chr8.4:72619777-72619796 | MS.gene25114:intron | 25.0% |
| ! | TGCATGTATATAAGAAGAAA+AGG | + | chr8.4:72619446-72619465 | MS.gene25114:intron | 25.0% |
| ! | TTATGGAATTAACTTTCTTG+TGG | + | chr8.4:72621883-72621902 | MS.gene25114:intron | 25.0% |
| ! | TTCAATGTTGAAGCTAATAA+TGG | + | chr8.4:72620436-72620455 | MS.gene25114:intron | 25.0% |
| !! | ACTTGATAATTTACATGAAG+AGG | - | chr8.4:72619251-72619270 | None:intergenic | 25.0% |
| !! | ATTCTTCCCATAATTCATTT+TGG | - | chr8.4:72620125-72620144 | None:intergenic | 25.0% |
| !! | CTATGAATGTTCTTGTTTAT+TGG | + | chr8.4:72619608-72619627 | MS.gene25114:intron | 25.0% |
| !! | CTTGATAATTTACATGAAGA+GGG | - | chr8.4:72619250-72619269 | None:intergenic | 25.0% |
| !! | CTTTTCACGTTAACATTAAT+GGG | + | chr8.4:72620082-72620101 | MS.gene25114:intron | 25.0% |
| !! | GAGTCTAAGCATTATATTTT+TGG | + | chr8.4:72620012-72620031 | MS.gene25114:intron | 25.0% |
| !! | GCAAGTAGATTTTATTACTT+TGG | + | chr8.4:72619849-72619868 | MS.gene25114:intron | 25.0% |
| !! | TATCACTTGGTTATTTCAAA+TGG | - | chr8.4:72620720-72620739 | None:intergenic | 25.0% |
| !! | TCTTTTCACGTTAACATTAA+TGG | + | chr8.4:72620081-72620100 | MS.gene25114:intron | 25.0% |
| !! | TTCTTCCCATAATTCATTTT+GGG | - | chr8.4:72620124-72620143 | None:intergenic | 25.0% |
| !! | TTGGTTATTTCAAATGGTAT+AGG | - | chr8.4:72620714-72620733 | None:intergenic | 25.0% |
| !! | TTTTATTACTTTGGCTAGAT+AGG | + | chr8.4:72619858-72619877 | MS.gene25114:intron | 25.0% |
| !! | TTTTGTAATTGTTCAAACTC+AGG | + | chr8.4:72620570-72620589 | MS.gene25114:intron | 25.0% |
| !! | TTTTTGCTTATAATAGTGAC+CGG | + | chr8.4:72619390-72619409 | MS.gene25114:intron | 25.0% |
| !! | TTTTTGCTTATATTAGTGAC+CGG | - | chr8.4:72619281-72619300 | None:intergenic | 25.0% |
| !!! | AGGTTCAAGTTTGTTTTTAT+GGG | + | chr8.4:72619746-72619765 | MS.gene25114:intron | 25.0% |
| !!! | ATGAATCTTCTACTTTTTGT+TGG | - | chr8.4:72622671-72622690 | None:intergenic | 25.0% |
| !!! | CCCTCACTTAATTTATTTTT+AGG | - | chr8.4:72621556-72621575 | None:intergenic | 25.0% |
| !!! | GTTAGTTAGAGTTTGTTATT+AGG | - | chr8.4:72622132-72622151 | None:intergenic | 25.0% |
| !!! | TAGGTTCAAGTTTGTTTTTA+TGG | + | chr8.4:72619745-72619764 | MS.gene25114:intron | 25.0% |
| !!! | TCATTTTACTACTGTTTTAG+AGG | - | chr8.4:72622186-72622205 | None:intergenic | 25.0% |
| !!! | TTTCTCACTCACTTTTTATT+AGG | + | chr8.4:72620628-72620647 | MS.gene25114:intron | 25.0% |
| AAGTGGTTCAAGGTATATAT+TGG | + | chr8.4:72621516-72621535 | MS.gene25114:intron | 30.0% | |
| AAGTTAAAAATTGGTTAGGG+AGG | + | chr8.4:72620168-72620187 | MS.gene25114:intron | 30.0% | |
| ACTTATCTACTTATCCTTCT+CGG | + | chr8.4:72619202-72619221 | MS.gene25114:CDS | 30.0% | |
| AGATAGGAGATATTCTTGTA+TGG | + | chr8.4:72619874-72619893 | MS.gene25114:intron | 30.0% | |
| ATGGAATTAACTTTCTTGTG+GGG | + | chr8.4:72621885-72621904 | MS.gene25114:intron | 30.0% | |
| ATTACAAAGACACACAACAT+AGG | + | chr8.4:72620814-72620833 | MS.gene25114:intron | 30.0% | |
| CATTCATCATAGCAACAAAT+AGG | + | chr8.4:72621754-72621773 | MS.gene25114:intron | 30.0% | |
| CGAAAAAGTTCTTTGAACAA+CGG | - | chr8.4:72621458-72621477 | None:intergenic | 30.0% | |
| CTGAGAGGTGAAAAAATATA+TGG | - | chr8.4:72619677-72619696 | None:intergenic | 30.0% | |
| CTTACAAAACCTTACATCTT+GGG | + | chr8.4:72621997-72622016 | MS.gene25114:intron | 30.0% | |
| GGCTTAAGTTACAATATCTT+AGG | + | chr8.4:72619052-72619071 | MS.gene25114:intron | 30.0% | |
| GTAATTTCAGATAGGTAGTT+TGG | - | chr8.4:72620799-72620818 | None:intergenic | 30.0% | |
| GTGAGTCAATATTTGTTTGA+TGG | + | chr8.4:72620242-72620261 | MS.gene25114:intron | 30.0% | |
| GTGTCTTTGTAATTTCAGAT+AGG | - | chr8.4:72620807-72620826 | None:intergenic | 30.0% | |
| TAGATAAGTTAACACCAAGA+GGG | - | chr8.4:72619191-72619210 | None:intergenic | 30.0% | |
| TATATACCTTGAACCACTTT+AGG | - | chr8.4:72621515-72621534 | None:intergenic | 30.0% | |
| TATCTTATTGTGTGCCTAAA+CGG | - | chr8.4:72619917-72619936 | None:intergenic | 30.0% | |
| TCAGTCTCTAATATTGTTCA+TGG | + | chr8.4:72619693-72619712 | MS.gene25114:intron | 30.0% | |
| TGAAATGGAATTTGGATCTT+TGG | - | chr8.4:72621057-72621076 | None:intergenic | 30.0% | |
| TGAAGAAATCCATATGAGAA+TGG | - | chr8.4:72622588-72622607 | None:intergenic | 30.0% | |
| ! | AATTTTGGACAATATGCGTA+TGG | + | chr8.4:72622626-72622645 | MS.gene25114:CDS | 30.0% |
| ! | AGAGAAGTCTAATCCTTTTA+GGG | - | chr8.4:72621030-72621049 | None:intergenic | 30.0% |
| ! | CATATTTTTCATACTGCAGA+TGG | - | chr8.4:72619828-72619847 | None:intergenic | 30.0% |
| ! | CTATTTGTTGCTATGATGAA+TGG | - | chr8.4:72621756-72621775 | None:intergenic | 30.0% |
| ! | GTGCATTTGATCAATTTTGA+AGG | - | chr8.4:72620777-72620796 | None:intergenic | 30.0% |
| ! | TACTAACTTGACCATTTTAG+AGG | + | chr8.4:72622781-72622800 | MS.gene25114:CDS | 30.0% |
| ! | TAGAGAAGTCTAATCCTTTT+AGG | - | chr8.4:72621031-72621050 | None:intergenic | 30.0% |
| ! | TTTTCAACAACGCCTTTTTA+TGG | + | chr8.4:72619149-72619168 | MS.gene25114:CDS | 30.0% |
| !! | ACCAAAAAAAAAAAGGCACT+AGG | + | chr8.4:72621113-72621132 | MS.gene25114:intron | 30.0% |
| !! | AGTAAGAGTATCATTCTTTG+CGG | - | chr8.4:72622765-72622784 | None:intergenic | 30.0% |
| !! | ATTGTGAGGGAGTTATTTTA+AGG | + | chr8.4:72619575-72619594 | MS.gene25114:intron | 30.0% |
| !! | ATTTTGGAAAGAACTTGTAG+AGG | + | chr8.4:72622487-72622506 | MS.gene25114:CDS | 30.0% |
| !! | CAAAAGTGCTTTCCATAAAA+AGG | - | chr8.4:72619164-72619183 | None:intergenic | 30.0% |
| !! | CAGCTGTCTTAATTTTGAAA+TGG | - | chr8.4:72621072-72621091 | None:intergenic | 30.0% |
| !! | CCAAAAAAAAAAAGGCACTA+GGG | + | chr8.4:72621114-72621133 | MS.gene25114:intron | 30.0% |
| !! | GTAGCTTTAGATCTCAAATT+CGG | - | chr8.4:72621329-72621348 | None:intergenic | 30.0% |
| !!! | AAAGAACTTTTTCGAACTGA+TGG | + | chr8.4:72621464-72621483 | MS.gene25114:CDS | 30.0% |
| !!! | ATCTTCTACTTTTTGTTGGT+CGG | - | chr8.4:72622667-72622686 | None:intergenic | 30.0% |
| !!! | CCCCTTGTGATTTTAATTTT+AGG | + | chr8.4:72619549-72619568 | MS.gene25114:intron | 30.0% |
| !!! | TTGTGAGGGAGTTATTTTAA+GGG | + | chr8.4:72619576-72619595 | MS.gene25114:intron | 30.0% |
| AAATTATCAAGTACACCCTC+CGG | + | chr8.4:72619259-72619278 | MS.gene25114:intron | 35.0% | |
| AACAAATAGGCATCTTAGTG+TGG | + | chr8.4:72621767-72621786 | MS.gene25114:intron | 35.0% | |
| ACTACCAGAAGACTTATTTG+AGG | + | chr8.4:72622732-72622751 | MS.gene25114:CDS | 35.0% | |
| ACTCCTCTAAATAACATGCA+AGG | - | chr8.4:72622296-72622315 | None:intergenic | 35.0% | |
| AGTTCTTTGAACAACGGTAT+TGG | - | chr8.4:72621452-72621471 | None:intergenic | 35.0% | |
| CAAGAAGTTTGGAAAGAAGT+TGG | + | chr8.4:72622895-72622914 | MS.gene25114:CDS | 35.0% | |
| CAAGTTCTTTCCAAAATGCT+TGG | - | chr8.4:72622484-72622503 | None:intergenic | 35.0% | |
| CCAATCCTTGTAAACAACAA+CGG | - | chr8.4:72621931-72621950 | None:intergenic | 35.0% | |
| CCTTACAAAACCTTACATCT+TGG | + | chr8.4:72621996-72622015 | MS.gene25114:intron | 35.0% | |
| CGTGATTGTGACACTAAAAT+CGG | + | chr8.4:72620344-72620363 | MS.gene25114:intron | 35.0% | |
| CTATACGTAAGTTTCACTTC+TGG | + | chr8.4:72620306-72620325 | MS.gene25114:intron | 35.0% | |
| CTTCTTTCCAAACTTCTTGT+AGG | - | chr8.4:72622894-72622913 | None:intergenic | 35.0% | |
| GAACAATATTAGAGACTGAG+AGG | - | chr8.4:72619692-72619711 | None:intergenic | 35.0% | |
| GATTGGAATTTCATTGAGCA+AGG | + | chr8.4:72621945-72621964 | MS.gene25114:intron | 35.0% | |
| GCGTTGTTGAAAAGTTTCAA+TGG | - | chr8.4:72619142-72619161 | None:intergenic | 35.0% | |
| GGGCAAACAAAGAAAAAGAA+AGG | + | chr8.4:72620925-72620944 | MS.gene25114:intron | 35.0% | |
| GTAGATAAGTTAACACCAAG+AGG | - | chr8.4:72619192-72619211 | None:intergenic | 35.0% | |
| GTAGGATAAAGCAGAGTATA+TGG | - | chr8.4:72622995-72623014 | None:intergenic | 35.0% | |
| GTGAAAGATCTTATTGAAGC+TGG | + | chr8.4:72619111-72619130 | MS.gene25114:CDS | 35.0% | |
| GTTCCAAGAGATGAATCATT+TGG | + | chr8.4:72621242-72621261 | MS.gene25114:intron | 35.0% | |
| GTTCTTTGAACAACGGTATT+GGG | - | chr8.4:72621451-72621470 | None:intergenic | 35.0% | |
| TAGGTGATAGTAACATTCTC+TGG | - | chr8.4:72620695-72620714 | None:intergenic | 35.0% | |
| TAGGTTATTGAAACGGCTTA+AGG | + | chr8.4:72619071-72619090 | MS.gene25114:intron | 35.0% | |
| TGAAGCTAATGTTTCACTTC+TGG | + | chr8.4:72620375-72620394 | MS.gene25114:intron | 35.0% | |
| TGACCAAATGATTCATCTCT+TGG | - | chr8.4:72621248-72621267 | None:intergenic | 35.0% | |
| TTAAAAGGATGCGTGTAAAG+TGG | + | chr8.4:72619031-72619050 | MS.gene25114:intron | 35.0% | |
| TTGAAGTCATCTGACTTTCT+TGG | + | chr8.4:72621269-72621288 | MS.gene25114:intron | 35.0% | |
| TTGTCCTCAAATAAGTCTTC+TGG | - | chr8.4:72622739-72622758 | None:intergenic | 35.0% | |
| ! | AAAGAAAAAAGTACACCCTC+CGG | - | chr8.4:72619412-72619431 | None:intergenic | 35.0% |
| ! | AATGCTGAAGCAGTTTTCAT+TGG | - | chr8.4:72620475-72620494 | None:intergenic | 35.0% |
| ! | AGAAGTCTAATCCTTTTAGG+GGG | - | chr8.4:72621028-72621047 | None:intergenic | 35.0% |
| ! | ATTCTGAACTCCAAGCATTT+TGG | + | chr8.4:72622471-72622490 | MS.gene25114:CDS | 35.0% |
| ! | CTTCATGCAGCTGTTAATTT+TGG | + | chr8.4:72622611-72622630 | MS.gene25114:CDS | 35.0% |
| ! | CTTCCTTGCATGTTATTTAG+AGG | + | chr8.4:72622290-72622309 | MS.gene25114:intron | 35.0% |
| ! | GAGAAGTCTAATCCTTTTAG+GGG | - | chr8.4:72621029-72621048 | None:intergenic | 35.0% |
| ! | GTTTAAGATGCAAACTCGTA+CGG | + | chr8.4:72622544-72622563 | MS.gene25114:CDS | 35.0% |
| ! | TTGCATGTTATTTAGAGGAG+TGG | + | chr8.4:72622295-72622314 | MS.gene25114:intron | 35.0% |
| !! | AATGTCCGTTGTTGTTTACA+AGG | + | chr8.4:72621923-72621942 | MS.gene25114:intron | 35.0% |
| !! | ATAAGTTGATTCCCCCTAAA+AGG | + | chr8.4:72621014-72621033 | MS.gene25114:intron | 35.0% |
| !! | CCAAGATGTAAGGTTTTGTA+AGG | - | chr8.4:72621999-72622018 | None:intergenic | 35.0% |
| !! | CTTCAAAGGTGTTAAACTCA+GGG | - | chr8.4:72621367-72621386 | None:intergenic | 35.0% |
| !! | GAATAGACTTCTGGTCTTAT+TGG | + | chr8.4:72620214-72620233 | MS.gene25114:intron | 35.0% |
| !! | TACTTTTTGTTGGTCGGTTA+AGG | - | chr8.4:72622661-72622680 | None:intergenic | 35.0% |
| !! | TCTTCAAAGGTGTTAAACTC+AGG | - | chr8.4:72621368-72621387 | None:intergenic | 35.0% |
| !! | TTCAGATGGACTAGAGATAT+GGG | + | chr8.4:72622379-72622398 | MS.gene25114:CDS | 35.0% |
| !!! | CCCTAGTGCCTTTTTTTTTT+TGG | - | chr8.4:72621117-72621136 | None:intergenic | 35.0% |
| !!! | TGTGAGGGAGTTATTTTAAG+GGG | + | chr8.4:72619577-72619596 | MS.gene25114:intron | 35.0% |
| !!! | TTATTTTTCGAGTGTCGTCT+TGG | - | chr8.4:72620998-72621017 | None:intergenic | 35.0% |
| AAACAGGCCTACAAGAAGTT+TGG | + | chr8.4:72622884-72622903 | MS.gene25114:CDS | 40.0% | |
| AACTATCTTGGTCAATGCAG+GGG | + | chr8.4:72621857-72621876 | MS.gene25114:intron | 40.0% | |
| AAGCTTGCACCATTCTCATA+TGG | + | chr8.4:72622576-72622595 | MS.gene25114:CDS | 40.0% | |
| ACAAACTGAGAAAGCCATGT+TGG | + | chr8.4:72620869-72620888 | MS.gene25114:intron | 40.0% | |
| ACTCTGCTTTATCCTACTAG+TGG | + | chr8.4:72622998-72623017 | MS.gene25114:CDS | 40.0% | |
| AGAAGATTCATGCCTGAGAA+AGG | + | chr8.4:72622680-72622699 | MS.gene25114:CDS | 40.0% | |
| ATACCTTGAACCACTTTAGG+TGG | - | chr8.4:72621512-72621531 | None:intergenic | 40.0% | |
| ATCGTGACTGAATAGACTTC+TGG | + | chr8.4:72620205-72620224 | MS.gene25114:intron | 40.0% | |
| ATTTACATGAAGAGGGTTCG+AGG | - | chr8.4:72619243-72619262 | None:intergenic | 40.0% | |
| CAAACTGAGAAAGCCATGTT+GGG | + | chr8.4:72620870-72620889 | MS.gene25114:intron | 40.0% | |
| CATCATTGTTCCTTTGAACG+AGG | - | chr8.4:72622937-72622956 | None:intergenic | 40.0% | |
| CTCTGCTTTATCCTACTAGT+GGG | + | chr8.4:72622999-72623018 | MS.gene25114:CDS | 40.0% | |
| GAACTATCTTGGTCAATGCA+GGG | + | chr8.4:72621856-72621875 | MS.gene25114:intron | 40.0% | |
| GACTATCCTTATGCTTCAGA+TGG | + | chr8.4:72622365-72622384 | MS.gene25114:CDS | 40.0% | |
| GAGACACTAAGGAATCGAAA+TGG | + | chr8.4:72622956-72622975 | MS.gene25114:CDS | 40.0% | |
| GCTTCTGATGAACAATACCT+TGG | + | chr8.4:72622818-72622837 | MS.gene25114:CDS | 40.0% | |
| GGAACAATGATGAGACACTA+AGG | + | chr8.4:72622945-72622964 | MS.gene25114:CDS | 40.0% | |
| TAAGTTTCCACCACCTAAAG+TGG | + | chr8.4:72621499-72621518 | MS.gene25114:CDS | 40.0% | |
| TAATCTGAACCAAACCGAGA+AGG | - | chr8.4:72619219-72619238 | None:intergenic | 40.0% | |
| TACTCAACAGATCCTTTCTC+AGG | - | chr8.4:72622695-72622714 | None:intergenic | 40.0% | |
| TATCTCCATCCTTTCCTGAT+AGG | - | chr8.4:72620972-72620991 | None:intergenic | 40.0% | |
| TCGAGAAAAACCTCGTTCAA+AGG | + | chr8.4:72622924-72622943 | MS.gene25114:CDS | 40.0% | |
| TCTACTTATCCTTCTCGGTT+TGG | + | chr8.4:72619207-72619226 | MS.gene25114:CDS | 40.0% | |
| TCTGCTTTATCCTACTAGTG+GGG | + | chr8.4:72623000-72623019 | MS.gene25114:CDS | 40.0% | |
| TGCTTATAATAGTGACCGGA+GGG | + | chr8.4:72619394-72619413 | MS.gene25114:intron | 40.0% | |
| TGCTTATATTAGTGACCGGA+GGG | - | chr8.4:72619277-72619296 | None:intergenic | 40.0% | |
| TGGAAAGAACTTGTAGAGGT+GGG | + | chr8.4:72622491-72622510 | MS.gene25114:CDS | 40.0% | |
| TTACCACTGAGAATCTGAGA+TGG | - | chr8.4:72622084-72622103 | None:intergenic | 40.0% | |
| TTCTGATTCACAGCCAAAAC+AGG | + | chr8.4:72622868-72622887 | MS.gene25114:CDS | 40.0% | |
| TTGCTTATAATAGTGACCGG+AGG | + | chr8.4:72619393-72619412 | MS.gene25114:intron | 40.0% | |
| TTGCTTATATTAGTGACCGG+AGG | - | chr8.4:72619278-72619297 | None:intergenic | 40.0% | |
| TTGGAAAGAACTTGTAGAGG+TGG | + | chr8.4:72622490-72622509 | MS.gene25114:CDS | 40.0% | |
| TTTGGACAATATGCGTATGG+AGG | + | chr8.4:72622629-72622648 | MS.gene25114:CDS | 40.0% | |
| ! | AACTTCTTGTAGGCCTGTTT+TGG | - | chr8.4:72622884-72622903 | None:intergenic | 40.0% |
| ! | ACTTTAGGAATGCAACATGG+TGG | + | chr8.4:72622522-72622541 | MS.gene25114:CDS | 40.0% |
| ! | ATCTGGCTTAGTACATCAGT+AGG | - | chr8.4:72621425-72621444 | None:intergenic | 40.0% |
| ! | ATGGGCTGCTATTAAGTCAT+GGG | + | chr8.4:72622397-72622416 | MS.gene25114:CDS | 40.0% |
| ! | CCTTGACAAGACCTCTAAAA+TGG | - | chr8.4:72622795-72622814 | None:intergenic | 40.0% |
| ! | GAGAGAGCGTATATCTTCAA+AGG | - | chr8.4:72621381-72621400 | None:intergenic | 40.0% |
| ! | GTGACTTTAGGAATGCAACA+TGG | + | chr8.4:72622519-72622538 | MS.gene25114:CDS | 40.0% |
| ! | TATGGGCTGCTATTAAGTCA+TGG | + | chr8.4:72622396-72622415 | MS.gene25114:CDS | 40.0% |
| ! | TCTAGTCCATCTGAAGCATA+AGG | - | chr8.4:72622374-72622393 | None:intergenic | 40.0% |
| !! | CCATTTTAGAGGTCTTGTCA+AGG | + | chr8.4:72622792-72622811 | MS.gene25114:CDS | 40.0% |
| !! | CCGTTGTTGTTTACAAGGAT+TGG | + | chr8.4:72621928-72621947 | MS.gene25114:intron | 40.0% |
| !! | CTTCAGATGGACTAGAGATA+TGG | + | chr8.4:72622378-72622397 | MS.gene25114:CDS | 40.0% |
| !! | TGAGTATGATGAGCTTGCTA+AGG | + | chr8.4:72622709-72622728 | MS.gene25114:CDS | 40.0% |
| !! | TTTTCGAACTGATGGTGAAG+CGG | + | chr8.4:72621472-72621491 | MS.gene25114:CDS | 40.0% |
| AAACCACGGCGGAGATTAAT+TGG | + | chr8.4:72618991-72619010 | MS.gene25114:intron | 45.0% | |
| ACTATCTTGGTCAATGCAGG+GGG | + | chr8.4:72621858-72621877 | MS.gene25114:intron | 45.0% | |
| AGAGTATATGGCACCTTCAC+AGG | - | chr8.4:72622983-72623002 | None:intergenic | 45.0% | |
| ATCACCAACTTGTCAGCCAT+TGG | + | chr8.4:72621613-72621632 | MS.gene25114:intron | 45.0% | |
| ATTGGTTAGGGAGGTGAACA+AGG | + | chr8.4:72620177-72620196 | MS.gene25114:intron | 45.0% | |
| ATTTAGAGGAGTGGCAGTTC+AGG | + | chr8.4:72622304-72622323 | MS.gene25114:intron | 45.0% | |
| CATACCAATGGCTGACAAGT+TGG | - | chr8.4:72621620-72621639 | None:intergenic | 45.0% | |
| CGAACTATCTTGGTCAATGC+AGG | + | chr8.4:72621855-72621874 | MS.gene25114:intron | 45.0% | |
| CTTTATCCTACTAGTGGGGA+AGG | + | chr8.4:72623004-72623023 | MS.gene25114:CDS | 45.0% | |
| GAATCGAAATGGACCTGTGA+AGG | + | chr8.4:72622967-72622986 | MS.gene25114:CDS | 45.0% | |
| GAGTGCAGCAGTTCAGTAAA+TGG | - | chr8.4:72620525-72620544 | None:intergenic | 45.0% | |
| GATATACGCTCTCTCTACGA+TGG | + | chr8.4:72621386-72621405 | MS.gene25114:CDS | 45.0% | |
| GCAAGGTCTCCATATTCAGA+CGG | + | chr8.4:72618935-72618954 | MS.gene25114:CDS | 45.0% | |
| GCAGTAGATAGTGTCTTGTG+TGG | - | chr8.4:72620046-72620065 | None:intergenic | 45.0% | |
| GTCTGAATATGGAGACCTTG+CGG | - | chr8.4:72618936-72618955 | None:intergenic | 45.0% | |
| TACCTTGGAGAGAGAATCGA+AGG | + | chr8.4:72622833-72622852 | MS.gene25114:CDS | 45.0% | |
| TTCATTGTGTCACGGTAGTG+TGG | - | chr8.4:72621819-72621838 | None:intergenic | 45.0% | |
| ! | ACAACGGTATTGGGCTAATC+TGG | - | chr8.4:72621442-72621461 | None:intergenic | 45.0% |
| ! | ATGGACTGACCCAAGATGTA+AGG | - | chr8.4:72622009-72622028 | None:intergenic | 45.0% |
| ! | TTCTTTTACCGCAGCCTATC+AGG | + | chr8.4:72620955-72620974 | MS.gene25114:intron | 45.0% |
| !!! | TGCTGCTTTTTGTGAAGTGC+AGG | - | chr8.4:72620846-72620865 | None:intergenic | 45.0% |
| AACCTTCCGCCGTCTGAATA+TGG | - | chr8.4:72618947-72618966 | None:intergenic | 50.0% | |
| AAGGTTCAAACGGTGAGGCT+AGG | + | chr8.4:72618961-72618980 | MS.gene25114:CDS | 50.0% | |
| AGAGAATCGAAGGCGATCTC+TGG | + | chr8.4:72622843-72622862 | MS.gene25114:CDS | 50.0% | |
| AGCCATGTTGGGATCTCTCT+CGG | + | chr8.4:72620881-72620900 | MS.gene25114:intron | 50.0% | |
| AGGTCTCCATATTCAGACGG+CGG | + | chr8.4:72618938-72618957 | MS.gene25114:CDS | 50.0% | |
| AGGTGGGTCATGGTGACTTT+AGG | + | chr8.4:72622507-72622526 | MS.gene25114:CDS | 50.0% | |
| ATACGCTCTCTCTACGATGG+TGG | + | chr8.4:72621389-72621408 | MS.gene25114:CDS | 50.0% | |
| ATCAGAAGTCGAACACCGTG+TGG | - | chr8.4:72622341-72622360 | None:intergenic | 50.0% | |
| CATCCTTTCCTGATAGGCTG+CGG | - | chr8.4:72620966-72620985 | None:intergenic | 50.0% | |
| CCACCACCTAAAGTGGTTCA+AGG | + | chr8.4:72621506-72621525 | MS.gene25114:CDS | 50.0% | |
| CCTTGAACCACTTTAGGTGG+TGG | - | chr8.4:72621509-72621528 | None:intergenic | 50.0% | |
| CGGTGTTCGACTTCTGATAG+AGG | + | chr8.4:72622343-72622362 | MS.gene25114:CDS | 50.0% | |
| GAACTTGTAGAGGTGGGTCA+TGG | + | chr8.4:72622497-72622516 | MS.gene25114:CDS | 50.0% | |
| GACCAAGATAGTTCGTGCGA+GGG | - | chr8.4:72621850-72621869 | None:intergenic | 50.0% | |
| GCTTGTGCTTGTTTGTGCGT+TGG | + | chr8.4:72620277-72620296 | MS.gene25114:intron | 50.0% | |
| GGCTAGGTAGCAAGAAACCA+CGG | + | chr8.4:72618977-72618996 | MS.gene25114:intron | 50.0% | |
| GTCAATCCTTCCCCACTAGT+AGG | - | chr8.4:72623013-72623032 | None:intergenic | 50.0% | |
| GTGCCATCTCAGATTCTCAG+TGG | + | chr8.4:72622078-72622097 | MS.gene25114:intron | 50.0% | |
| GTGCTTGTTTGTGCGTTGGA+AGG | + | chr8.4:72620281-72620300 | MS.gene25114:intron | 50.0% | |
| GTTTGCCCGCTAGTTGTTTG+TGG | - | chr8.4:72620913-72620932 | None:intergenic | 50.0% | |
| TAGGTAGCAAGAAACCACGG+CGG | + | chr8.4:72618980-72618999 | MS.gene25114:intron | 50.0% | |
| TCCCTGCCACAAACAACTAG+CGG | + | chr8.4:72620904-72620923 | MS.gene25114:intron | 50.0% | |
| TGAATATGGAGACCTTGCGG+CGG | - | chr8.4:72618933-72618952 | None:intergenic | 50.0% | |
| TGACCAAGATAGTTCGTGCG+AGG | - | chr8.4:72621851-72621870 | None:intergenic | 50.0% | |
| TTACCGCAGCCTATCAGGAA+AGG | + | chr8.4:72620960-72620979 | MS.gene25114:intron | 50.0% | |
| TTCCCTCGCACGAACTATCT+TGG | + | chr8.4:72621845-72621864 | MS.gene25114:intron | 50.0% | |
| !! | AGCACTTTTGCTGTCCCTCT+TGG | + | chr8.4:72619174-72619193 | MS.gene25114:CDS | 50.0% |
| AATATGGAGACCTTGCGGCG+GGG | - | chr8.4:72618931-72618950 | None:intergenic | 55.0% | |
| CAGACGGCGGAAGGTTCAAA+CGG | + | chr8.4:72618951-72618970 | MS.gene25114:CDS | 55.0% | |
| CAGGATCCAGCTTCTCCACA+CGG | + | chr8.4:72622323-72622342 | MS.gene25114:CDS | 55.0% | |
| CCACCAATTAATCTCCGCCG+TGG | - | chr8.4:72618997-72619016 | None:intergenic | 55.0% | |
| CCACGGCGGAGATTAATTGG+TGG | + | chr8.4:72618994-72619013 | MS.gene25114:intron | 55.0% | |
| CCCGCTAGTTGTTTGTGGCA+GGG | - | chr8.4:72620908-72620927 | None:intergenic | 55.0% | |
| CCCTGCCACAAACAACTAGC+GGG | + | chr8.4:72620905-72620924 | MS.gene25114:intron | 55.0% | |
| CGCAGCCTATCAGGAAAGGA+TGG | + | chr8.4:72620964-72620983 | MS.gene25114:intron | 55.0% | |
| CGCCTTCGATTCTCTCTCCA+AGG | - | chr8.4:72622838-72622857 | None:intergenic | 55.0% | |
| CTCCATATTCAGACGGCGGA+AGG | + | chr8.4:72618942-72618961 | MS.gene25114:CDS | 55.0% | |
| GAATATGGAGACCTTGCGGC+GGG | - | chr8.4:72618932-72618951 | None:intergenic | 55.0% | |
| GACACTGTTGGGAATGCCTC+TGG | - | chr8.4:72623038-72623057 | None:intergenic | 55.0% | |
| GACCGAGAGAGATCCCAACA+TGG | - | chr8.4:72620886-72620905 | None:intergenic | 55.0% | |
| ! | GGGGAAGGATTGACTTCCAG+AGG | + | chr8.4:72623019-72623038 | MS.gene25114:CDS | 55.0% |
| !! | GGTCAATGCAGGGGGTGTTA+TGG | + | chr8.4:72621866-72621885 | MS.gene25114:intron | 55.0% |
| CGAACACCGTGTGGAGAAGC+TGG | - | chr8.4:72622332-72622351 | None:intergenic | 60.0% | |
| GCCCGCTAGTTGTTTGTGGC+AGG | - | chr8.4:72620909-72620928 | None:intergenic | 60.0% | |
| GGCGGAAGGTTCAAACGGTG+AGG | + | chr8.4:72618956-72618975 | MS.gene25114:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 72618921 | 72623066 | 72618921 | ID=MS.gene25114 |
| chr8.4 | mRNA | 72618921 | 72623066 | 72618921 | ID=MS.gene25114.t1;Parent=MS.gene25114 |
| chr8.4 | exon | 72618921 | 72618982 | 72618921 | ID=MS.gene25114.t1.exon1;Parent=MS.gene25114.t1 |
| chr8.4 | CDS | 72618921 | 72618982 | 72618921 | ID=cds.MS.gene25114.t1;Parent=MS.gene25114.t1 |
| chr8.4 | exon | 72619074 | 72619256 | 72619074 | ID=MS.gene25114.t1.exon2;Parent=MS.gene25114.t1 |
| chr8.4 | CDS | 72619074 | 72619256 | 72619074 | ID=cds.MS.gene25114.t1;Parent=MS.gene25114.t1 |
| chr8.4 | exon | 72621319 | 72621527 | 72621319 | ID=MS.gene25114.t1.exon3;Parent=MS.gene25114.t1 |
| chr8.4 | CDS | 72621319 | 72621527 | 72621319 | ID=cds.MS.gene25114.t1;Parent=MS.gene25114.t1 |
| chr8.4 | exon | 72622312 | 72623066 | 72622312 | ID=MS.gene25114.t1.exon4;Parent=MS.gene25114.t1 |
| chr8.4 | CDS | 72622312 | 72623066 | 72622312 | ID=cds.MS.gene25114.t1;Parent=MS.gene25114.t1 |
| Gene Sequence |
| Protein sequence |
