Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25237.t1 | XP_013450312.1 | 96.8 | 408 | 10 | 1 | 1 | 405 | 1 | 408 | 4.00E-214 | 753.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25237.t1 | Q8LB17 | 68.3 | 404 | 121 | 4 | 7 | 405 | 1 | 402 | 3.7e-147 | 522.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25237.t1 | A0A072UEY4 | 96.8 | 408 | 10 | 1 | 1 | 405 | 1 | 408 | 2.9e-214 | 753.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene25237 | 0.823215 | 1.56E-53 | -1.69E-46 |
| MS.gene049711 | MS.gene25237 | 0.835728 | 1.42E-56 | -1.69E-46 |
| MS.gene049807 | MS.gene25237 | 0.845626 | 3.61E-59 | -1.69E-46 |
| MS.gene049850 | MS.gene25237 | 0.801233 | 9.92E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene25237 | 0.826817 | 2.20E-54 | -1.69E-46 |
| MS.gene050561 | MS.gene25237 | 0.820603 | 6.29E-53 | -1.69E-46 |
| MS.gene050562 | MS.gene25237 | 0.814935 | 1.20E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene25237 | 0.831648 | 1.48E-55 | -1.69E-46 |
| MS.gene051719 | MS.gene25237 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene25237 | 0.807094 | 5.98E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene25237 | 0.837567 | 4.82E-57 | -1.69E-46 |
| MS.gene052155 | MS.gene25237 | -0.820661 | 6.10E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene25237 | 0.859256 | 4.69E-63 | -1.69E-46 |
| MS.gene052533 | MS.gene25237 | 0.813263 | 2.80E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene25237 | 0.855958 | 4.45E-62 | -1.69E-46 |
| MS.gene053536 | MS.gene25237 | 0.803619 | 3.20E-49 | -1.69E-46 |
| MS.gene053538 | MS.gene25237 | 0.804101 | 2.54E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene25237 | 0.801223 | 9.97E-49 | -1.69E-46 |
| MS.gene053599 | MS.gene25237 | 0.81317 | 2.93E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene25237 | 0.837559 | 4.84E-57 | -1.69E-46 |
| MS.gene054651 | MS.gene25237 | 0.84733 | 1.24E-59 | -1.69E-46 |
| MS.gene055465 | MS.gene25237 | 0.808697 | 2.73E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene25237 | 0.815007 | 1.15E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene25237 | 0.817306 | 3.53E-52 | -1.69E-46 |
| MS.gene055580 | MS.gene25237 | -0.815407 | 9.39E-52 | -1.69E-46 |
| MS.gene055890 | MS.gene25237 | 0.813687 | 2.26E-51 | -1.69E-46 |
| MS.gene055891 | MS.gene25237 | 0.808898 | 2.47E-50 | -1.69E-46 |
| MS.gene056169 | MS.gene25237 | 0.800697 | 1.28E-48 | -1.69E-46 |
| MS.gene056366 | MS.gene25237 | 0.812145 | 4.91E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene25237 | 0.812278 | 4.59E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene25237 | 0.819285 | 1.26E-52 | -1.69E-46 |
| MS.gene05894 | MS.gene25237 | 0.821202 | 4.58E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene25237 | 0.860999 | 1.40E-63 | -1.69E-46 |
| MS.gene060587 | MS.gene25237 | 0.807492 | 4.92E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25237.t1 | MTR_7g107390 | 96.814 | 408 | 10 | 1 | 1 | 405 | 1 | 408 | 0.0 | 800 |
| MS.gene25237.t1 | MTR_7g107390 | 97.118 | 347 | 7 | 1 | 1 | 344 | 1 | 347 | 0.0 | 677 |
| MS.gene25237.t1 | MTR_4g123573 | 32.500 | 160 | 102 | 4 | 55 | 213 | 60 | 214 | 2.00e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25237.t1 | AT3G58460 | 68.627 | 408 | 113 | 6 | 7 | 405 | 1 | 402 | 0.0 | 563 |
| MS.gene25237.t1 | AT3G58460 | 68.000 | 400 | 113 | 6 | 15 | 405 | 32 | 425 | 0.0 | 547 |
| MS.gene25237.t1 | AT3G17611 | 33.333 | 159 | 103 | 3 | 55 | 213 | 61 | 216 | 5.71e-15 | 75.9 |
| MS.gene25237.t1 | AT3G59520 | 29.817 | 218 | 136 | 7 | 32 | 243 | 15 | 221 | 1.23e-12 | 68.2 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51725 | gene | 18832 | 27023 | 18832 | ID=MS.gene25237 |
| 51725 | mRNA | 18832 | 27023 | 18832 | ID=MS.gene25237.t1;Parent=MS.gene25237 |
| 51725 | exon | 18832 | 18873 | 18832 | ID=MS.gene25237.t1.exon1;Parent=MS.gene25237.t1 |
| 51725 | CDS | 18832 | 18873 | 18832 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 19017 | 19182 | 19017 | ID=MS.gene25237.t1.exon2;Parent=MS.gene25237.t1 |
| 51725 | CDS | 19017 | 19182 | 19017 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 19696 | 19999 | 19696 | ID=MS.gene25237.t1.exon3;Parent=MS.gene25237.t1 |
| 51725 | CDS | 19696 | 19999 | 19696 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 22753 | 22785 | 22753 | ID=MS.gene25237.t1.exon4;Parent=MS.gene25237.t1 |
| 51725 | CDS | 22753 | 22785 | 22753 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 23138 | 23229 | 23138 | ID=MS.gene25237.t1.exon5;Parent=MS.gene25237.t1 |
| 51725 | CDS | 23138 | 23229 | 23138 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 23332 | 23409 | 23332 | ID=MS.gene25237.t1.exon6;Parent=MS.gene25237.t1 |
| 51725 | CDS | 23332 | 23409 | 23332 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 24174 | 24255 | 24174 | ID=MS.gene25237.t1.exon7;Parent=MS.gene25237.t1 |
| 51725 | CDS | 24174 | 24255 | 24174 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 24343 | 24418 | 24343 | ID=MS.gene25237.t1.exon8;Parent=MS.gene25237.t1 |
| 51725 | CDS | 24343 | 24418 | 24343 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 25314 | 25471 | 25314 | ID=MS.gene25237.t1.exon9;Parent=MS.gene25237.t1 |
| 51725 | CDS | 25314 | 25471 | 25314 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 26461 | 26512 | 26461 | ID=MS.gene25237.t1.exon10;Parent=MS.gene25237.t1 |
| 51725 | CDS | 26461 | 26512 | 26461 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 26602 | 26658 | 26602 | ID=MS.gene25237.t1.exon11;Parent=MS.gene25237.t1 |
| 51725 | CDS | 26602 | 26658 | 26602 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| 51725 | exon | 26949 | 27023 | 26949 | ID=MS.gene25237.t1.exon12;Parent=MS.gene25237.t1 |
| 51725 | CDS | 26949 | 27023 | 26949 | ID=cds.MS.gene25237.t1;Parent=MS.gene25237.t1 |
| Gene Sequence |
| Protein sequence |
