Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25241.t1 | XP_013450314.1 | 100 | 44 | 0 | 0 | 1 | 44 | 1 | 44 | 4.90E-16 | 92.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25241.t1 | O22518 | 97.7 | 43 | 1 | 0 | 2 | 44 | 10 | 52 | 1.7e-17 | 89.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25241.t1 | G8A187 | 100.0 | 44 | 0 | 0 | 1 | 44 | 1 | 44 | 3.5e-16 | 92.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene25241 | -0.801097 | 1.06E-48 | -1.69E-46 |
| MS.gene050105 | MS.gene25241 | -0.828885 | 7.01E-55 | -1.69E-46 |
| MS.gene051122 | MS.gene25241 | -0.838699 | 2.46E-57 | -1.69E-46 |
| MS.gene051924 | MS.gene25241 | 0.910128 | 2.65E-82 | -1.69E-46 |
| MS.gene051926 | MS.gene25241 | 0.962616 | 4.33E-121 | -1.69E-46 |
| MS.gene052333 | MS.gene25241 | -0.813641 | 2.31E-51 | -1.69E-46 |
| MS.gene052674 | MS.gene25241 | -0.814006 | 1.92E-51 | -1.69E-46 |
| MS.gene052675 | MS.gene25241 | -0.809146 | 2.19E-50 | -1.69E-46 |
| MS.gene052677 | MS.gene25241 | -0.814205 | 1.73E-51 | -1.69E-46 |
| MS.gene052965 | MS.gene25241 | 0.81834 | 2.06E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene25241 | 0.804413 | 2.19E-49 | -1.69E-46 |
| MS.gene054950 | MS.gene25241 | 0.810308 | 1.23E-50 | -1.69E-46 |
| MS.gene055453 | MS.gene25241 | 0.828818 | 7.27E-55 | -1.69E-46 |
| MS.gene055455 | MS.gene25241 | 0.805356 | 1.39E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene25241 | -0.825469 | 4.61E-54 | -1.69E-46 |
| MS.gene056064 | MS.gene25241 | -0.801587 | 8.39E-49 | -1.69E-46 |
| MS.gene056779 | MS.gene25241 | -0.826244 | 3.02E-54 | -1.69E-46 |
| MS.gene057026 | MS.gene25241 | -0.803847 | 2.87E-49 | -1.69E-46 |
| MS.gene057156 | MS.gene25241 | 0.847082 | 1.45E-59 | -1.69E-46 |
| MS.gene058223 | MS.gene25241 | 0.817461 | 3.26E-52 | -1.69E-46 |
| MS.gene059952 | MS.gene25241 | -0.808086 | 3.68E-50 | -1.69E-46 |
| MS.gene060879 | MS.gene25241 | -0.810026 | 1.41E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25241.t1 | MTR_7g107400 | 100.000 | 44 | 0 | 0 | 1 | 44 | 1 | 44 | 3.42e-26 | 96.3 |
| MS.gene25241.t1 | MTR_3g095810 | 97.500 | 40 | 1 | 0 | 5 | 44 | 9 | 48 | 2.53e-22 | 85.9 |
| MS.gene25241.t1 | MTR_3g095810 | 97.500 | 40 | 1 | 0 | 5 | 44 | 9 | 48 | 2.54e-22 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25241.t1 | AT3G04770 | 80.952 | 42 | 8 | 0 | 3 | 44 | 9 | 50 | 2.05e-20 | 80.9 |
| MS.gene25241.t1 | AT3G04770 | 79.070 | 43 | 9 | 0 | 2 | 44 | 8 | 50 | 5.62e-20 | 80.1 |
| MS.gene25241.t1 | AT1G72370 | 76.744 | 43 | 10 | 0 | 2 | 44 | 7 | 49 | 4.52e-19 | 77.4 |
| MS.gene25241.t1 | AT1G72370 | 76.744 | 43 | 10 | 0 | 2 | 44 | 7 | 49 | 5.01e-19 | 77.4 |
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATTTAGGGTTTGGAAAA+TGG | 0.189223 | 7.1:+5929733 | None:intergenic |
| AAATTGGATAATTTAGGGTT+TGG | 0.349997 | 7.1:+5929726 | None:intergenic |
| CATCGGCGGCAAGCATCATC+TGG | 0.408335 | 7.1:+5929830 | None:intergenic |
| GGCAAGCATCATCTGGATAT+CGG | 0.457963 | 7.1:+5929837 | None:intergenic |
| ATCTTCAAGCGCCGTAACGA+TGG | 0.529765 | 7.1:-5929763 | MS.gene25241:CDS |
| ATGGAATGTTACCATCGTTA+CGG | 0.545997 | 7.1:+5929752 | None:intergenic |
| TTTAGTACCGAGATGGACAT+CGG | 0.561895 | 7.1:+5929813 | None:intergenic |
| AAGTTGACGAGGGGCAGCCA+TGG | 0.573667 | 7.1:+5929873 | None:intergenic |
| CTTTCTGAGAAAGTTGACGA+GGG | 0.588586 | 7.1:+5929863 | None:intergenic |
| CTTGCCGCCGATGTCCATCT+CGG | 0.588724 | 7.1:-5929820 | MS.gene25241:CDS |
| TCTTTCTGAGAAAGTTGACG+AGG | 0.624055 | 7.1:+5929862 | None:intergenic |
| TTTCTGAGAAAGTTGACGAG+GGG | 0.682900 | 7.1:+5929864 | None:intergenic |
| AGTACCGAGATGGACATCGG+CGG | 0.738432 | 7.1:+5929816 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGGAATGTTACCATCGTTA+CGG | + | chr7.1:5929860-5929879 | None:intergenic | 35.0% | |
| TAAAAACTGCGACTTTCAGA+TGG | - | chr7.1:5929815-5929834 | MS.gene25241:CDS | 35.0% | |
| ! | CTTTCTGAGAAAGTTGACGA+GGG | + | chr7.1:5929749-5929768 | None:intergenic | 40.0% |
| ! | TCTTTCTGAGAAAGTTGACG+AGG | + | chr7.1:5929750-5929769 | None:intergenic | 40.0% |
| ! | TTTAGTACCGAGATGGACAT+CGG | + | chr7.1:5929799-5929818 | None:intergenic | 40.0% |
| ! | TTTCTGAGAAAGTTGACGAG+GGG | + | chr7.1:5929748-5929767 | None:intergenic | 40.0% |
| GGCAAGCATCATCTGGATAT+CGG | + | chr7.1:5929775-5929794 | None:intergenic | 45.0% | |
| !!! | CGCAGTTTTTAGTACCGAGA+TGG | + | chr7.1:5929806-5929825 | None:intergenic | 45.0% |
| ! | ATCTTCAAGCGCCGTAACGA+TGG | - | chr7.1:5929846-5929865 | MS.gene25241:CDS | 50.0% |
| ! | AGTACCGAGATGGACATCGG+CGG | + | chr7.1:5929796-5929815 | None:intergenic | 55.0% |
| CATCGGCGGCAAGCATCATC+TGG | + | chr7.1:5929782-5929801 | None:intergenic | 60.0% | |
| CTTGCCGCCGATGTCCATCT+CGG | - | chr7.1:5929789-5929808 | MS.gene25241:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 5929738 | 5929893 | 5929738 | ID=MS.gene25241 |
| chr7.1 | mRNA | 5929738 | 5929893 | 5929738 | ID=MS.gene25241.t1;Parent=MS.gene25241 |
| chr7.1 | exon | 5929738 | 5929893 | 5929738 | ID=MS.gene25241.t1.exon1;Parent=MS.gene25241.t1 |
| chr7.1 | CDS | 5929738 | 5929893 | 5929738 | ID=cds.MS.gene25241.t1;Parent=MS.gene25241.t1 |
| Gene Sequence |
| Protein sequence |
