AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26285


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26285.t1 MTR_5g075020 98.592 142 2 0 1 142 1 142 6.36e-105 299
MS.gene26285.t1 MTR_5g075020 99.242 132 1 0 11 142 7 138 4.04e-97 279
MS.gene26285.t1 MTR_3g094690 76.087 138 25 3 10 141 6 141 2.19e-72 216
MS.gene26285.t1 MTR_5g083960 79.389 131 22 3 12 142 9 134 5.77e-70 209
MS.gene26285.t1 MTR_3g094690 75.362 138 25 4 10 141 6 140 7.16e-70 210
MS.gene26285.t1 MTR_2g068760 67.647 102 33 0 39 140 5 106 1.14e-48 154
MS.gene26285.t1 MTR_4g083680 72.632 95 26 0 46 140 14 108 2.39e-48 153
MS.gene26285.t1 MTR_5g083230 71.579 95 27 0 46 140 5 99 3.23e-48 153
MS.gene26285.t1 MTR_3g071590 68.367 98 31 0 43 140 4 101 2.13e-47 150
MS.gene26285.t1 MTR_2g093310 63.918 97 35 0 44 140 30 126 1.38e-43 142
MS.gene26285.t1 MTR_7g033800 67.010 97 32 0 46 142 10 106 3.54e-43 140
MS.gene26285.t1 MTR_3g031660 64.211 95 34 0 46 140 6 100 3.80e-43 140
MS.gene26285.t1 MTR_4g060950 61.111 108 40 2 35 140 11 118 8.78e-43 140
MS.gene26285.t1 MTR_4g105170 67.010 97 32 0 46 142 10 106 9.75e-43 140
MS.gene26285.t1 MTR_3g077240 64.948 97 34 0 46 142 9 105 4.41e-42 138
MS.gene26285.t1 MTR_6g005070 63.918 97 35 0 46 142 8 104 4.65e-42 136
MS.gene26285.t1 MTR_3g073690 62.887 97 36 0 46 142 8 104 1.03e-40 135
MS.gene26285.t1 MTR_1g095850 64.516 93 33 0 48 140 73 165 1.23e-40 135
MS.gene26285.t1 MTR_3g071420 58.879 107 38 1 34 140 7 107 1.49e-40 134
MS.gene26285.t1 MTR_7g074990 67.368 95 30 1 47 140 20 114 1.64e-40 135
MS.gene26285.t1 MTR_6g005080 61.856 97 37 0 46 142 9 105 2.72e-40 132
MS.gene26285.t1 MTR_5g017950 62.500 96 36 0 46 141 7 102 2.95e-39 134
MS.gene26285.t1 MTR_5g083010 57.944 107 39 1 34 140 4 104 3.53e-39 130
MS.gene26285.t1 MTR_7g096530 57.143 105 44 1 37 140 4 108 2.40e-38 129
MS.gene26285.t1 MTR_3g452660 60.000 95 38 0 46 140 6 100 1.94e-37 129
MS.gene26285.t1 MTR_8g040900 65.854 82 28 0 46 127 6 87 5.98e-37 126
MS.gene26285.t1 MTR_7g028905 56.989 93 40 0 48 140 6 98 1.51e-35 119
MS.gene26285.t1 MTR_1g070220 58.947 95 38 1 47 140 15 109 7.41e-35 120
MS.gene26285.t1 MTR_8g036105 55.140 107 47 1 35 140 6 112 3.86e-34 119
MS.gene26285.t1 MTR_8g036085 61.053 95 36 1 47 140 23 117 7.30e-34 118
MS.gene26285.t1 MTR_6g027710 55.670 97 42 1 45 140 38 134 3.90e-33 116
MS.gene26285.t1 MTR_1g070205 57.292 96 38 2 47 140 12 106 1.98e-32 115
MS.gene26285.t1 MTR_5g093010 53.763 93 43 0 48 140 6 98 2.80e-32 112
MS.gene26285.t1 MTR_7g096610 56.701 97 39 2 46 140 7 102 7.17e-32 113
MS.gene26285.t1 MTR_5g080470 53.125 96 44 1 46 140 6 101 1.73e-31 111
MS.gene26285.t1 MTR_8g079660 48.958 96 49 0 45 140 6 101 3.09e-28 104
MS.gene26285.t1 MTR_7g075230 54.639 97 41 2 47 140 12 108 4.41e-28 102
MS.gene26285.t1 MTR_6g027700 47.368 95 49 1 47 140 7 101 2.62e-27 101
MS.gene26285.t1 MTR_8g079580 48.454 97 49 1 45 140 6 102 9.59e-25 95.1
MS.gene26285.t1 MTR_4g107450 44.444 99 55 0 44 142 7 105 1.66e-24 95.9
MS.gene26285.t1 MTR_6g011250 42.105 95 54 1 48 141 10 104 3.09e-24 92.4
MS.gene26285.t1 MTR_8g079620 43.750 96 54 0 45 140 6 101 5.50e-24 93.2
MS.gene26285.t1 MTR_6g011230 39.000 100 61 0 43 142 7 106 3.58e-23 90.1
MS.gene26285.t1 MTR_6g011200 39.806 103 58 2 42 140 1 103 8.05e-23 90.5
MS.gene26285.t1 MTR_2g100020 41.414 99 56 2 44 141 16 113 4.56e-21 86.3
MS.gene26285.t1 MTR_8g017090 39.796 98 57 2 44 140 9 105 7.84e-20 83.6
MS.gene26285.t1 MTR_5g085390 35.922 103 62 2 42 140 1 103 6.56e-19 79.7
MS.gene26285.t1 MTR_4g099230 40.000 80 47 1 48 126 3 82 5.44e-18 75.9
MS.gene26285.t1 MTR_4g088035 34.091 88 58 0 46 133 5 92 1.06e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26285.t1 AT2G30340 84.211 114 16 2 28 140 33 145 2.52e-65 200
MS.gene26285.t1 AT2G30340 84.211 114 16 2 28 140 34 146 2.57e-65 200
MS.gene26285.t1 AT2G40470 73.723 137 30 4 10 142 6 140 8.45e-59 182
MS.gene26285.t1 AT2G40470 81.416 113 19 1 30 142 5 115 1.25e-57 178
MS.gene26285.t1 AT2G30130 69.474 95 29 0 46 140 7 101 6.88e-46 148
MS.gene26285.t1 AT1G31320 76.136 88 21 0 46 133 12 99 1.21e-45 146
MS.gene26285.t1 AT1G07900 63.918 97 35 0 44 140 30 126 1.24e-43 142
MS.gene26285.t1 AT2G28500 62.887 97 36 0 44 140 52 148 4.93e-43 142
MS.gene26285.t1 AT3G27650 64.948 97 34 0 44 140 36 132 2.75e-42 137
MS.gene26285.t1 AT1G16530 62.245 98 36 1 44 140 11 108 3.58e-42 137
MS.gene26285.t1 AT5G63090 64.948 97 34 0 46 142 10 106 4.21e-41 135
MS.gene26285.t1 AT5G63090 64.948 97 34 0 46 142 10 106 4.21e-41 135
MS.gene26285.t1 AT5G63090 64.948 97 34 0 46 142 10 106 4.21e-41 135
MS.gene26285.t1 AT5G63090 64.948 97 34 0 46 142 10 106 4.21e-41 135
MS.gene26285.t1 AT2G23660 61.053 95 37 0 46 140 4 98 1.09e-39 135
MS.gene26285.t1 AT2G23660 61.053 95 37 0 46 140 4 98 1.09e-39 135
MS.gene26285.t1 AT2G23660 61.053 95 37 0 46 140 4 98 1.09e-39 135
MS.gene26285.t1 AT5G66870 63.158 95 35 0 46 140 6 100 1.99e-39 135
MS.gene26285.t1 AT1G65620 68.235 85 27 0 43 127 5 89 1.60e-38 129
MS.gene26285.t1 AT1G65620 68.235 85 27 0 43 127 5 89 1.60e-38 129
MS.gene26285.t1 AT1G65620 68.235 85 27 0 43 127 5 89 1.60e-38 129
MS.gene26285.t1 AT1G65620 68.235 85 27 0 43 127 5 89 1.60e-38 129
MS.gene26285.t1 AT1G65620 68.235 85 27 0 43 127 5 89 1.60e-38 129
MS.gene26285.t1 AT3G11090 63.441 93 34 0 48 140 12 104 8.07e-38 126
MS.gene26285.t1 AT3G26620 58.065 93 39 0 48 140 6 98 3.69e-37 123
MS.gene26285.t1 AT3G26660 58.065 93 39 0 48 140 6 98 4.12e-37 123
MS.gene26285.t1 AT2G42430 56.731 104 44 1 38 140 6 109 1.03e-36 126
MS.gene26285.t1 AT4G00210 56.731 104 44 1 38 140 2 105 5.74e-36 123
MS.gene26285.t1 AT3G03760 59.375 96 38 1 46 140 50 145 5.32e-34 120
MS.gene26285.t1 AT5G06080 57.292 96 40 1 46 140 6 101 3.63e-33 115
MS.gene26285.t1 AT2G31310 55.208 96 42 1 46 140 6 101 4.78e-33 115
MS.gene26285.t1 AT3G58190 58.333 96 37 2 47 140 11 105 6.96e-33 115
MS.gene26285.t1 AT4G00220 63.043 92 33 1 50 140 20 111 7.58e-33 115
MS.gene26285.t1 AT2G45420 57.609 92 38 1 50 140 40 131 1.12e-31 114
MS.gene26285.t1 AT2G42440 54.639 97 41 2 46 140 6 101 8.32e-31 111
MS.gene26285.t1 AT2G45410 56.522 92 39 1 50 140 19 110 1.44e-30 108
MS.gene26285.t1 AT3G50510 47.368 95 50 0 46 140 11 105 4.58e-26 97.8
MS.gene26285.t1 AT3G50510 47.368 95 50 0 46 140 11 105 4.58e-26 97.8
MS.gene26285.t1 AT3G47870 40.909 110 58 1 33 142 29 131 3.36e-24 95.5
MS.gene26285.t1 AT5G35900 37.113 97 61 0 46 142 4 100 1.87e-20 83.2
MS.gene26285.t1 AT1G72980 39.796 98 59 0 44 141 10 107 2.31e-20 83.2
MS.gene26285.t1 AT1G06280 39.362 94 57 0 48 141 25 118 3.26e-20 82.8
MS.gene26285.t1 AT3G13850 38.710 93 57 0 48 140 37 129 1.45e-19 82.0
MS.gene26285.t1 AT2G19820 34.831 89 57 1 47 134 12 100 7.13e-15 67.0
MS.gene26285.t1 AT4G22700 39.216 102 51 4 48 140 6 105 8.17e-15 68.2
MS.gene26285.t1 AT5G15060 40.260 77 42 2 45 118 15 90 8.24e-12 59.3
MS.gene26285.t1 AT1G36000 30.000 100 68 2 42 140 4 102 6.58e-11 56.6
MS.gene26285.t1 AT2G19510 27.368 95 69 0 47 141 9 103 9.44e-11 55.8

Find 24 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAGGGGTTACTGTGTTTA+AGG 0.163285 5.4:-59341711 None:intergenic
AGGTTAAGAGATCCTGTTTA+TGG 0.207855 5.4:+59342196 MS.gene26285:CDS
CATCTTTATGGTCCAACTTC+TGG 0.211009 5.4:+59341683 MS.gene26285:CDS
GCATCTGCTCTTTGTCCTTC+AGG 0.259282 5.4:-59342142 None:intergenic
CAATGTCTCTAAATTGCTAT+TGG 0.302262 5.4:+59341847 MS.gene26285:CDS
AAACAATCAACAAGCATGTT+TGG 0.327582 5.4:+59341373 None:intergenic
ATGGGAAGGAAGCATCTTTA+TGG 0.328269 5.4:+59341671 MS.gene26285:CDS
GTAGTTCTGCTTGTAAAGAT+TGG 0.343171 5.4:-59342255 None:intergenic
GGGAGAAACTTCTTCTCAAA+TGG 0.345005 5.4:+59341652 MS.gene26285:CDS
ATCTTTATGGTCCAACTTCT+GGG 0.351664 5.4:+59341684 MS.gene26285:CDS
AGCAGATGCAGCAAATAGTA+TGG 0.405210 5.4:+59342156 MS.gene26285:CDS
TATTTACCTGTCACTGTCTC+TGG 0.409777 5.4:-59341400 None:intergenic
GCACCCATGCATCCATAAAC+AGG 0.433076 5.4:-59342208 None:intergenic
TTTAAGGTTGTCCCAGAAGT+TGG 0.446561 5.4:-59341695 None:intergenic
GGAGAAACTTCTTCTCAAAT+GGG 0.481100 5.4:+59341653 MS.gene26285:CDS
TGGTTTATGAAGCTAATTTG+AGG 0.520361 5.4:+59342176 MS.gene26285:CDS
GGATGTCCAGAGACAGTGAC+AGG 0.566988 5.4:+59341394 MS.gene26285:CDS
AATAGCTTACATGCAGCACA+AGG 0.568241 5.4:-59341728 None:intergenic
AGATCCTGTTTATGGATGCA+TGG 0.578296 5.4:+59342204 MS.gene26285:CDS
AAACTTCTTCTCAAATGGGA+AGG 0.578669 5.4:+59341657 MS.gene26285:CDS
TAGCTTACATGCAGCACAAG+GGG 0.625764 5.4:-59341726 None:intergenic
GATCCTGTTTATGGATGCAT+GGG 0.637525 5.4:+59342205 MS.gene26285:CDS
ATTGAGAAGAAGATGTGCTG+AGG 0.667195 5.4:+59341748 MS.gene26285:CDS
ATAGCTTACATGCAGCACAA+GGG 0.685250 5.4:-59341727 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTGAAATATTTTTTTTT+AGG + chr5.4:59342114-59342133 MS.gene26285:intron 10.0%
!! AATATATATATTTCTCAACA+AGG + chr5.4:59341546-59341565 MS.gene26285:intron 15.0%
!!! TTGAAATATTTTTTTTTAGG+AGG + chr5.4:59342117-59342136 MS.gene26285:intron 15.0%
!!! TTTTATGTAAGAAATGAAAA+GGG - chr5.4:59341453-59341472 None:intergenic 15.0%
!!! TTTTTATGTAAGAAATGAAA+AGG - chr5.4:59341454-59341473 None:intergenic 15.0%
!! TATTTCATCTAATCTTTCTT+TGG - chr5.4:59341615-59341634 None:intergenic 20.0%
! ACTGATCTAACGATTATAAT+TGG + chr5.4:59342046-59342065 MS.gene26285:intron 25.0%
! ATAAATCTAACATCGATACA+CGG + chr5.4:59341931-59341950 MS.gene26285:intron 25.0%
! TATATTTCTCAACAAGGTTA+AGG + chr5.4:59341552-59341571 MS.gene26285:intron 25.0%
!! ATCAGTAAATACTATTGACT+TGG - chr5.4:59342032-59342051 None:intergenic 25.0%
!! TGAGATTTTTACTATGTTCT+TGG + chr5.4:59341494-59341513 MS.gene26285:intron 25.0%
!!! CTAATTTTGAGTTATGAGTT+AGG - chr5.4:59341879-59341898 None:intergenic 25.0%
!!! TCATAACTATTTCTGAAAGT+CGG - chr5.4:59342009-59342028 None:intergenic 25.0%
AATAGCAAAAATCAACTCAG+AGG + chr5.4:59341631-59341650 MS.gene26285:CDS 30.0%
ATAGCAAAAATCAACTCAGA+GGG + chr5.4:59341632-59341651 MS.gene26285:CDS 30.0%
CAATGTCTCTAAATTGCTAT+TGG + chr5.4:59341847-59341866 MS.gene26285:CDS 30.0%
GTGAAAAATAAGGAGAGAAA+GGG - chr5.4:59341778-59341797 None:intergenic 30.0%
TGGTTTATGAAGCTAATTTG+AGG + chr5.4:59342176-59342195 MS.gene26285:CDS 30.0%
!! AACGATTATAATTGGTTTGC+AGG + chr5.4:59342054-59342073 MS.gene26285:intron 30.0%
!! ACGATTATAATTGGTTTGCA+GGG + chr5.4:59342055-59342074 MS.gene26285:intron 30.0%
AAACTTCTTCTCAAATGGGA+AGG + chr5.4:59341657-59341676 MS.gene26285:CDS 35.0%
AGGTTAAGAGATCCTGTTTA+TGG + chr5.4:59342196-59342215 MS.gene26285:CDS 35.0%
ATCTTTATGGTCCAACTTCT+GGG + chr5.4:59341684-59341703 MS.gene26285:CDS 35.0%
GGAGAAACTTCTTCTCAAAT+GGG + chr5.4:59341653-59341672 MS.gene26285:CDS 35.0%
GGTGAAAAATAAGGAGAGAA+AGG - chr5.4:59341779-59341798 None:intergenic 35.0%
TAAGAAATGAAAAGGGAGCT+AGG - chr5.4:59341446-59341465 None:intergenic 35.0%
! GCAGCTGTTCATAAAGTTTT+TGG + chr5.4:59341818-59341837 MS.gene26285:CDS 35.0%
! GTAGTTCTGCTTGTAAAGAT+TGG - chr5.4:59342258-59342277 None:intergenic 35.0%
! TGAACAGCTGCAAATTTTTG+AGG - chr5.4:59341809-59341828 None:intergenic 35.0%
!!! TTTTTTTAGGAGGTACCTGA+AGG + chr5.4:59342127-59342146 MS.gene26285:intron 35.0%
AATAGCTTACATGCAGCACA+AGG - chr5.4:59341731-59341750 None:intergenic 40.0%
ACAAGGGGTTACTGTGTTTA+AGG - chr5.4:59341714-59341733 None:intergenic 40.0%
AGATCCTGTTTATGGATGCA+TGG + chr5.4:59342204-59342223 MS.gene26285:CDS 40.0%
AGCAGATGCAGCAAATAGTA+TGG + chr5.4:59342156-59342175 MS.gene26285:CDS 40.0%
ATAGCTTACATGCAGCACAA+GGG - chr5.4:59341730-59341749 None:intergenic 40.0%
ATCTAACATCGATACACGGT+CGG + chr5.4:59341935-59341954 MS.gene26285:intron 40.0%
ATTGAGAAGAAGATGTGCTG+AGG + chr5.4:59341748-59341767 MS.gene26285:CDS 40.0%
CATCTTTATGGTCCAACTTC+TGG + chr5.4:59341683-59341702 MS.gene26285:CDS 40.0%
GATCCTGTTTATGGATGCAT+GGG + chr5.4:59342205-59342224 MS.gene26285:CDS 40.0%
GCTTAGTGCATGATTAGAAG+AGG - chr5.4:59341526-59341545 None:intergenic 40.0%
GGCTCATGTGGTGAAAAATA+AGG - chr5.4:59341788-59341807 None:intergenic 40.0%
GGGAGAAACTTCTTCTCAAA+TGG + chr5.4:59341652-59341671 MS.gene26285:CDS 40.0%
TATTTACCTGTCACTGTCTC+TGG - chr5.4:59341403-59341422 None:intergenic 40.0%
! TTTAAGGTTGTCCCAGAAGT+TGG - chr5.4:59341698-59341717 None:intergenic 40.0%
!! ATGGGAAGGAAGCATCTTTA+TGG + chr5.4:59341671-59341690 MS.gene26285:CDS 40.0%
!!! GCAAATTTTTGAGGCTCATG+TGG - chr5.4:59341800-59341819 None:intergenic 40.0%
TAGCTTACATGCAGCACAAG+GGG - chr5.4:59341729-59341748 None:intergenic 45.0%
ACATCGATACACGGTCGGTT+AGG + chr5.4:59341940-59341959 MS.gene26285:intron 50.0%
GCACCCATGCATCCATAAAC+AGG - chr5.4:59342211-59342230 None:intergenic 50.0%
GCATCTGCTCTTTGTCCTTC+AGG - chr5.4:59342145-59342164 None:intergenic 50.0%
GGATGTCCAGAGACAGTGAC+AGG + chr5.4:59341394-59341413 MS.gene26285:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 59341388 59342282 59341388 ID=MS.gene26285
chr5.4 mRNA 59341388 59342282 59341388 ID=MS.gene26285.t1;Parent=MS.gene26285
chr5.4 exon 59341388 59341415 59341388 ID=MS.gene26285.t1.exon1;Parent=MS.gene26285.t1
chr5.4 CDS 59341388 59341415 59341388 ID=cds.MS.gene26285.t1;Parent=MS.gene26285.t1
chr5.4 exon 59341618 59341868 59341618 ID=MS.gene26285.t1.exon2;Parent=MS.gene26285.t1
chr5.4 CDS 59341618 59341868 59341618 ID=cds.MS.gene26285.t1;Parent=MS.gene26285.t1
chr5.4 exon 59342136 59342282 59342136 ID=MS.gene26285.t1.exon3;Parent=MS.gene26285.t1
chr5.4 CDS 59342136 59342282 59342136 ID=cds.MS.gene26285.t1;Parent=MS.gene26285.t1
Gene Sequence

>MS.gene26285

ATGTTTGGATGTCCAGAGACAGTGACAGGTAAATATTAGCAAATACACATAATTTCCTAGCTCCCTTTTCATTTCTTACATAAAAAAAACAAAAAAAAATTATTCATGAGATTTTTACTATGTTCTTGGTCTCTACCTCTTCTAATCATGCACTAAGCAATATATATATTTCTCAACAAGGTTAAGGATACTATTTAACATGCTATTTTTGTGTTTACTTTTTGCCAAAGAAAGATTAGATGAAATAGCAAAAATCAACTCAGAGGGAGAAACTTCTTCTCAAATGGGAAGGAAGCATCTTTATGGTCCAACTTCTGGGACAACCTTAAACACAGTAACCCCTTGTGCTGCATGTAAGCTATTGAGAAGAAGATGTGCTGAGGAATGCCCTTTCTCTCCTTATTTTTCACCACATGAGCCTCAAAAATTTGCAGCTGTTCATAAAGTTTTTGGTGCTAGCAATGTCTCTAAATTGCTATTGGTTTGTGCCTAACTCATAACTCAAAATTAGTTACTTTTATTATCTTCAATAACTTATAAGTAATAAATCTAACATCGATACACGGTCGGTTAGGTAAAAACTTTTACACTACGAACAAATCACAATCAGTCATATGTCCGACTTTCAGAAATAGTTATGACCAAGTCAATAGTATTTACTGATCTAACGATTATAATTGGTTTGCAGGGTAAAAAAGATAAGTAATAACTAATGATTTGCTGTCAACTTTGAAATATTTTTTTTTAGGAGGTACCTGAAGGACAAAGAGCAGATGCAGCAAATAGTATGGTTTATGAAGCTAATTTGAGGTTAAGAGATCCTGTTTATGGATGCATGGGTGCAATATCATCATTACAACAACAAATCCAATCTTTACAAGCAGAACTACATGCA

Protein sequence

>MS.gene26285.t1

MFGCPETVTERLDEIAKINSEGETSSQMGRKHLYGPTSGTTLNTVTPCAACKLLRRRCAEECPFSPYFSPHEPQKFAAVHKVFGASNVSKLLLEVPEGQRADAANSMVYEANLRLRDPVYGCMGAISSLQQQIQSLQAELHA