AlfalfaGEDB | MS.gene26402

M. sativa cultivar XinJiangDaYe / MS.gene26402

KEGG: K22588
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0008168 GO:0008171 GO:0016740 GO:0016741 GO:0046983
PFAM: PF00891.21 PF08100.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26402.t1	XP_013456930.1	98.6	364	4	1	1	363	1	364	8.40E-203	716.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26402.t1	Q29U70	98.6	364	4	1	1	363	1	364	2.0e-205	716.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26402.t1	A0A072UNK8	98.6	364	4	1	1	363	1	364	6.0e-203	716.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050381	MS.gene26402	0.827093	1.89E-54	-1.69E-46
MS.gene050883	MS.gene26402	0.851484	8.61E-61	-1.69E-46
MS.gene051127	MS.gene26402	0.844112	9.25E-59	-1.69E-46
MS.gene055124	MS.gene26402	0.816128	6.49E-52	-1.69E-46
MS.gene055961	MS.gene26402	0.812378	4.37E-51	-1.69E-46
MS.gene056310	MS.gene26402	0.84897	4.36E-60	-1.69E-46
MS.gene056311	MS.gene26402	0.834274	3.29E-56	-1.69E-46
MS.gene056313	MS.gene26402	0.835487	1.63E-56	-1.69E-46
MS.gene058099	MS.gene26402	0.819726	9.98E-53	-1.69E-46
MS.gene058108	MS.gene26402	0.809806	1.58E-50	-1.69E-46
MS.gene058109	MS.gene26402	0.85135	9.40E-61	-1.69E-46
MS.gene058111	MS.gene26402	0.826873	2.14E-54	-1.69E-46
MS.gene058171	MS.gene26402	0.853999	1.65E-61	-1.69E-46
MS.gene058173	MS.gene26402	0.856398	3.31E-62	-1.69E-46
MS.gene058175	MS.gene26402	0.857483	1.58E-62	-1.69E-46
MS.gene058628	MS.gene26402	0.813192	2.90E-51	-1.69E-46
MS.gene058629	MS.gene26402	0.814773	1.30E-51	-1.69E-46
MS.gene058630	MS.gene26402	0.821281	4.39E-53	-1.69E-46
MS.gene059045	MS.gene26402	0.81787	2.63E-52	-1.69E-46
MS.gene061157	MS.gene26402	0.812266	4.62E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26402.t1	MTR_4g088190	98.626	364	4	1	1	363	1	364	0.0	732
MS.gene26402.t1	MTR_4g088200	88.981	363	38	1	1	363	1	361	0.0	665
MS.gene26402.t1	MTR_4g033015	75.000	364	89	2	1	363	1	363	0.0	543
MS.gene26402.t1	MTR_4g033150	74.451	364	91	2	1	363	1	363	0.0	540
MS.gene26402.t1	MTR_4g033200	74.725	364	90	2	1	363	1	363	0.0	540
MS.gene26402.t1	MTR_4g033170	74.105	363	88	1	1	363	1	357	0.0	520
MS.gene26402.t1	MTR_4g033085	74.656	363	86	1	1	363	1	357	0.0	506
MS.gene26402.t1	MTR_0794s0020	72.800	250	66	2	1	249	1	249	8.14e-133	379
MS.gene26402.t1	MTR_7g014510	49.587	363	177	4	1	363	1	357	1.23e-121	355
MS.gene26402.t1	MTR_7g014570	49.587	363	172	5	1	363	1	352	3.83e-121	353
MS.gene26402.t1	MTR_5g074600	49.859	355	170	5	11	363	11	359	3.86e-121	354
MS.gene26402.t1	MTR_4g088345	52.473	364	164	5	1	363	1	356	7.13e-120	350
MS.gene26402.t1	MTR_7g024500	48.876	356	170	5	6	361	5	348	4.23e-118	346
MS.gene26402.t1	MTR_5g074680	48.023	354	176	5	11	362	12	359	2.86e-117	344
MS.gene26402.t1	MTR_1g076940	49.180	366	178	6	1	363	1	361	7.54e-117	343
MS.gene26402.t1	MTR_7g024520	50.000	356	166	5	6	361	5	348	4.19e-116	341
MS.gene26402.t1	MTR_5g074580	48.732	355	174	5	11	363	12	360	1.44e-115	340
MS.gene26402.t1	MTR_4g079160	46.893	354	179	3	11	363	10	355	4.80e-109	323
MS.gene26402.t1	MTR_4g088340	51.320	341	157	5	24	363	1	333	1.25e-107	318
MS.gene26402.t1	MTR_5g074610	45.892	353	158	3	11	363	7	326	1.03e-106	316
MS.gene26402.t1	MTR_5g074540	45.352	355	168	5	11	363	11	341	1.75e-105	313
MS.gene26402.t1	MTR_3g083620	36.337	344	202	10	20	359	27	357	8.63e-61	199
MS.gene26402.t1	MTR_4g038440	34.060	367	226	9	2	363	13	368	8.80e-61	200
MS.gene26402.t1	MTR_0017s0100	33.880	366	228	8	2	363	13	368	6.42e-59	195
MS.gene26402.t1	MTR_0079s0070	34.211	342	209	9	27	363	4	334	1.19e-56	188
MS.gene26402.t1	MTR_1g076930	41.975	243	132	5	24	263	1	237	7.90e-56	183
MS.gene26402.t1	MTR_5g098170	34.441	331	199	10	28	350	30	350	3.22e-44	156
MS.gene26402.t1	MTR_1g036460	31.487	343	211	10	33	362	38	369	1.50e-43	154
MS.gene26402.t1	MTR_1g036490	31.858	339	215	8	33	362	38	369	3.31e-43	154
MS.gene26402.t1	MTR_3g092900	32.486	354	205	11	9	349	17	349	8.51e-42	150
MS.gene26402.t1	MTR_7g012090	31.045	335	214	8	26	350	34	361	1.21e-41	150
MS.gene26402.t1	MTR_7g012070	29.851	335	218	8	26	350	34	361	4.46e-40	145
MS.gene26402.t1	MTR_7g012040	30.395	329	218	7	26	350	33	354	9.06e-40	144
MS.gene26402.t1	MTR_1g096310	31.977	344	211	10	26	358	34	365	1.08e-39	144
MS.gene26402.t1	MTR_2g072790	33.140	344	208	10	26	360	21	351	1.49e-39	144
MS.gene26402.t1	MTR_0591s0010	32.099	324	205	9	28	344	43	358	3.13e-39	143
MS.gene26402.t1	MTR_8g068750	32.024	331	204	9	28	348	43	362	4.19e-39	143
MS.gene26402.t1	MTR_7g011990	28.448	348	223	9	26	362	34	366	4.83e-39	142
MS.gene26402.t1	MTR_7g011900	30.931	333	212	8	26	350	34	356	7.15e-39	142
MS.gene26402.t1	MTR_7g118320	31.325	332	209	11	26	348	26	347	1.35e-38	141
MS.gene26402.t1	MTR_8g068690	32.209	326	206	9	26	344	40	357	1.87e-37	139
MS.gene26402.t1	MTR_8g068870	30.141	355	230	10	4	348	2	348	2.73e-37	138
MS.gene26402.t1	MTR_2g055940	31.579	323	206	9	32	348	34	347	1.10e-36	136
MS.gene26402.t1	MTR_7g118300	30.205	341	213	10	26	355	42	368	4.05e-35	132
MS.gene26402.t1	MTR_7g012020	29.429	333	217	8	26	350	33	355	2.01e-34	130
MS.gene26402.t1	MTR_1g036510	29.688	320	196	9	56	362	80	383	2.33e-34	130
MS.gene26402.t1	MTR_8g024160	40.000	185	106	3	173	355	140	321	8.98e-34	127
MS.gene26402.t1	MTR_3g021430	30.994	342	211	10	27	358	24	350	1.47e-33	127
MS.gene26402.t1	MTR_4g038440	32.331	266	168	6	2	263	13	270	1.78e-33	125
MS.gene26402.t1	MTR_3g021440	30.383	339	206	12	28	353	38	359	2.42e-33	127
MS.gene26402.t1	MTR_3g096050	29.499	339	219	11	32	362	31	357	9.62e-33	125
MS.gene26402.t1	MTR_8g068570	29.299	314	176	8	27	331	42	318	6.84e-31	119
MS.gene26402.t1	MTR_8g068730	28.529	333	185	9	26	348	41	330	1.74e-28	114
MS.gene26402.t1	MTR_6g078850	27.586	348	232	9	9	348	33	368	1.74e-28	116
MS.gene26402.t1	MTR_3g096070	27.083	336	204	10	32	360	31	332	9.63e-26	106
MS.gene26402.t1	MTR_7g012020	29.150	247	166	6	26	267	33	275	1.31e-22	96.7
MS.gene26402.t1	MTR_1g050220	34.000	150	86	2	198	347	151	287	5.86e-21	92.0
MS.gene26402.t1	MTR_3g096050	30.290	241	153	9	32	266	31	262	1.68e-17	81.6
MS.gene26402.t1	MTR_8g068690	27.935	247	167	7	26	266	40	281	1.89e-17	82.0
MS.gene26402.t1	MTR_8g068740	27.076	277	153	7	26	293	41	277	3.17e-17	81.3
MS.gene26402.t1	MTR_1g036510	28.767	219	132	7	56	263	80	285	1.05e-15	77.0
MS.gene26402.t1	MTR_8g068600	26.971	241	149	9	38	264	27	254	1.43e-15	76.6
MS.gene26402.t1	MTR_7g012020	29.167	216	144	6	26	236	33	244	1.58e-14	73.2
MS.gene26402.t1	MTR_4g025570	45.783	83	38	3	6	88	16	91	1.69e-14	72.4
MS.gene26402.t1	MTR_4g025570	38.542	96	55	3	265	359	122	214	1.44e-12	66.6
MS.gene26402.t1	MTR_8g068620	37.234	94	59	0	173	266	20	113	3.51e-14	68.9
MS.gene26402.t1	MTR_8g068760	42.529	87	46	2	263	348	135	218	9.72e-13	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26402.t1	AT4G35150	33.520	358	178	11	16	362	15	323	1.98e-50	171
MS.gene26402.t1	AT5G54160	34.582	347	196	9	28	361	31	359	6.69e-47	163
MS.gene26402.t1	AT4G35160	34.435	363	211	11	16	362	29	380	1.62e-46	163
MS.gene26402.t1	AT3G53140	32.571	350	207	13	26	360	21	356	1.16e-40	147
MS.gene26402.t1	AT1G77520	31.283	374	224	13	9	362	20	380	4.53e-38	140
MS.gene26402.t1	AT1G51990	30.749	374	228	12	4	363	6	362	2.14e-37	138
MS.gene26402.t1	AT1G51990	30.481	374	229	12	4	363	6	362	2.44e-37	138
MS.gene26402.t1	AT5G53810	31.624	351	212	12	29	362	38	377	7.96e-34	129
MS.gene26402.t1	AT1G33030	30.571	350	210	14	28	362	18	349	2.56e-33	127
MS.gene26402.t1	AT1G77530	32.337	368	228	11	9	362	20	380	3.26e-33	127
MS.gene26402.t1	AT1G63140	28.763	372	237	12	9	362	19	380	2.72e-31	122
MS.gene26402.t1	AT1G63140	28.763	372	237	12	9	362	39	400	3.44e-31	122
MS.gene26402.t1	AT1G76790	27.933	358	218	12	7	344	9	346	3.52e-25	105
MS.gene26402.t1	AT1G62900	34.896	192	120	3	173	362	16	204	7.43e-25	100
MS.gene26402.t1	AT5G37170	30.928	194	128	4	172	362	43	233	1.46e-23	98.2
MS.gene26402.t1	AT5G37170	30.928	194	128	4	172	362	143	333	2.90e-23	99.4
MS.gene26402.t1	AT5G37170	30.851	188	124	4	178	362	1	185	3.68e-22	92.8
MS.gene26402.t1	AT1G21130	27.089	347	200	15	31	350	40	360	2.53e-21	94.4
MS.gene26402.t1	AT1G21110	26.023	342	200	14	31	345	40	355	2.00e-20	91.7
MS.gene26402.t1	AT1G21100	26.163	344	207	13	31	350	40	360	2.27e-20	91.7
MS.gene26402.t1	AT1G21120	25.581	344	209	12	31	350	40	360	5.04e-20	90.5
MS.gene26402.t1	AT1G21120	25.581	344	209	12	31	350	88	408	6.25e-20	90.9
MS.gene26402.t1	AT1G63140	26.568	271	176	9	9	263	39	302	9.87e-14	71.6
MS.gene26402.t1	AT1G33030	28.000	250	151	12	28	263	18	252	1.87e-12	67.4
MS.gene26402.t1	AT1G33030	27.935	247	149	12	31	263	21	252	2.47e-12	67.0

Find 64 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGTTCTTCAATATCTTTA+AGG	0.195840	4.1:+22131726	None:intergenic
GTGCAACAGGGGAGAGCTTT+TGG	0.222303	4.1:-22132404	MS.gene26402:CDS
TTTGAAACTTCATCCTTCTA+AGG	0.260291	4.1:-22132679	MS.gene26402:CDS
CGAACACGCGTTTGTTCTCT+TGG	0.271846	4.1:+22132292	None:intergenic
CAAACGCGTGTTCGAGGGTT+TGG	0.283225	4.1:-22132283	MS.gene26402:CDS
TGTTTATCTTCAATTGTTAA+AGG	0.290462	4.1:-22132510	MS.gene26402:CDS
CAGGTTGTTGGTAACTTAAC+TGG	0.291153	4.1:-22132165	MS.gene26402:CDS
TACAGTGGGTTCTGCATGAT+TGG	0.326455	4.1:-22131762	MS.gene26402:intron
AGAAACCGTTGTGTGTTAAA+AGG	0.336053	4.1:+22132631	None:intergenic
TGTATACATGTTTGTACAAA+TGG	0.340652	4.1:+22132805	None:intergenic
ACACTGTTTGAGTGTGCAAC+AGG	0.344866	4.1:-22132417	MS.gene26402:CDS
GTGATGCTTACAATGTTTCT+TGG	0.364082	4.1:-22131589	MS.gene26402:CDS
TGGATTAACTGAACTGCAAT+TGG	0.377228	4.1:-22131623	MS.gene26402:CDS
AATGAAAATTTGAACTTTGT+TGG	0.379769	4.1:-22132141	MS.gene26402:CDS
AAAACAGTGCATTTAAGGTT+AGG	0.380135	4.1:+22132198	None:intergenic
GCTAATTCAGAAAGAGTCAT+TGG	0.394069	4.1:+22132708	None:intergenic
CCACTTATGAGAAGCTTTGA+AGG	0.396791	4.1:+22132543	None:intergenic
GTTGATGTTGGAGGTGGTAC+TGG	0.401447	4.1:-22132252	MS.gene26402:CDS
TTGGAGTCTCTTGTTGATGT+TGG	0.404723	4.1:-22132264	MS.gene26402:CDS
CTCCACATGTCTAAAGAACT+TGG	0.410016	4.1:+22132477	None:intergenic
TGAAGTATATTAACCTTAGA+AGG	0.411294	4.1:+22132666	None:intergenic
AAGAAAGAACAAAGCAAGAA+TGG	0.426132	4.1:-22131564	MS.gene26402:CDS
ACTGCAATTGGACTATGATT+TGG	0.427028	4.1:-22131611	MS.gene26402:CDS
GAGAGACTTGAAGCCAGAAA+TGG	0.427559	4.1:+22131495	None:intergenic
CCTTCAAAGCTTCTCATAAG+TGG	0.434600	4.1:-22132543	MS.gene26402:CDS
AGAGACTTGAAGCCAGAAAT+GGG	0.439445	4.1:+22131496	None:intergenic
AGAGAACAAACGCGTGTTCG+AGG	0.444395	4.1:-22132289	MS.gene26402:CDS
GTGTTAAAAGGCGCAAGAAT+CGG	0.481292	4.1:+22132643	None:intergenic
GAAAATTTGAACTTTGTTGG+TGG	0.485249	4.1:-22132138	MS.gene26402:CDS
TTCACTTTCTTCAGAACCAT+TGG	0.487182	4.1:+22132845	None:intergenic
TGTGGAGTTCCTCAGAGAAA+TGG	0.491399	4.1:-22132461	MS.gene26402:CDS
TTTGATCAACCACAGGTTGT+TGG	0.492105	4.1:-22132177	MS.gene26402:CDS
CTCAAATCAGCCATGGAACT+AGG	0.496080	4.1:-22132762	MS.gene26402:CDS
GAGTCTCTTGTTGATGTTGG+AGG	0.496314	4.1:-22132261	MS.gene26402:CDS
ATCCAAGTTCTTTAGACATG+TGG	0.496638	4.1:-22132479	MS.gene26402:CDS
GAGTATGTTTCAAGATGCTA+TGG	0.498973	4.1:-22132346	MS.gene26402:CDS
ACAATAGTGAAAGGCAAAGA+AGG	0.501253	4.1:-22132600	MS.gene26402:CDS
ATCAGCTATGCCTAGTTCCA+TGG	0.501316	4.1:+22132752	None:intergenic
AGAAAGAACAAAGCAAGAAT+GGG	0.507206	4.1:-22131563	MS.gene26402:CDS
GAAATGGTTCAATGAAGATA+AGG	0.516608	4.1:-22132445	MS.gene26402:CDS
ACAATTGAAGATAAACATGT+TGG	0.521606	4.1:+22132516	None:intergenic
ATACAACTTTGTTAGTTCCA+TGG	0.535416	4.1:-22132787	MS.gene26402:CDS
GAAATGGCTTTCACTACCAA+TGG	0.538698	4.1:-22132861	MS.gene26402:CDS
GAGAACAAACGCGTGTTCGA+GGG	0.542946	4.1:-22132288	MS.gene26402:CDS
ATTTCACACAAAGGAAAGGA+TGG	0.547784	4.1:-22131694	MS.gene26402:CDS
AGTAAAACAGCATCAGCAGA+AGG	0.551999	4.1:+22132096	None:intergenic
TCTCTTGTTGATGTTGGAGG+TGG	0.554073	4.1:-22132258	MS.gene26402:CDS
AGGATGAAGTTTCAAAGAAG+AGG	0.557817	4.1:+22132686	None:intergenic
CATGGCTGATTTGAGTGCCA+TGG	0.563511	4.1:+22132770	None:intergenic
AGCAATTTCACACAAAGGAA+AGG	0.577136	4.1:-22131698	MS.gene26402:CDS
CACTGTTTGAGTGTGCAACA+GGG	0.603315	4.1:-22132416	MS.gene26402:CDS
CATGGCACTCAAATCAGCCA+TGG	0.607444	4.1:-22132769	MS.gene26402:CDS
GATGTTGGAGGTGGTACTGG+TGG	0.610342	4.1:-22132249	MS.gene26402:CDS
TGTACAAATGGATTTGAGCA+TGG	0.612036	4.1:+22132817	None:intergenic
TTCATTGAACCATTTCTCTG+AGG	0.619737	4.1:+22132452	None:intergenic
GTAAAACAGCATCAGCAGAA+GGG	0.623492	4.1:+22132097	None:intergenic
AAAGAAGCAATTTCACACAA+AGG	0.626707	4.1:-22131703	MS.gene26402:CDS
GCTGATGCAATTCACAACCA+TGG	0.632132	4.1:-22132735	MS.gene26402:CDS
GAGACTTGAAGCCAGAAATG+GGG	0.636393	4.1:+22131497	None:intergenic
AGACTTGAAGCCAGAAATGG+GGG	0.646680	4.1:+22131498	None:intergenic
ACTGTTTGAGTGTGCAACAG+GGG	0.649035	4.1:-22132415	MS.gene26402:CDS
GATGAAACAAGTGACGATCG+TGG	0.671140	4.1:-22131643	MS.gene26402:CDS
CTTATGAGAAGCTTTGAAGG+AGG	0.681784	4.1:+22132546	None:intergenic
GTTAAGTTACCAACAACCTG+TGG	0.693381	4.1:+22132168	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATATAACTTTTACTAATA+AGG	-	chr4.1:22132466-22132485	MS.gene26402:CDS	10.0%
!!!	AATTGTTACTATTTCAATTT+TGG	-	chr4.1:22132281-22132300	MS.gene26402:CDS	15.0%
!!!	TGACATTTAATTTAGTTATT+TGG	+	chr4.1:22132541-22132560	None:intergenic	15.0%
!!!	TTTTACTAATAAGGATAAAA+TGG	-	chr4.1:22132475-22132494	MS.gene26402:CDS	15.0%
!!	AAGTTATATAACAAGATTGA+AGG	+	chr4.1:22132457-22132476	None:intergenic	20.0%
!!	AATGAAAATTTGAACTTTGT+TGG	-	chr4.1:22132194-22132213	MS.gene26402:CDS	20.0%
!!	TGTTTATCTTCAATTGTTAA+AGG	-	chr4.1:22131825-22131844	MS.gene26402:intron	20.0%
!!!	ACAAATTAAGATGCATATTT+TGG	-	chr4.1:22132510-22132529	MS.gene26402:CDS	20.0%
!	ACAATTGAAGATAAACATGT+TGG	+	chr4.1:22131822-22131841	None:intergenic	25.0%
!	TGAAGTATATTAACCTTAGA+AGG	+	chr4.1:22131672-22131691	None:intergenic	25.0%
!	TGTATACATGTTTGTACAAA+TGG	+	chr4.1:22131533-22131552	None:intergenic	25.0%
!	TGTTAGACATTAAAATGTGT+AGG	-	chr4.1:22132423-22132442	MS.gene26402:CDS	25.0%
!	TTTGCAAAAACAATAGTGAA+AGG	-	chr4.1:22131726-22131745	MS.gene26402:CDS	25.0%
!!	CAATTGTCTTTTATTACAGT+GGG	-	chr4.1:22132559-22132578	MS.gene26402:CDS	25.0%
!!	TCAATTGTCTTTTATTACAG+TGG	-	chr4.1:22132558-22132577	MS.gene26402:CDS	25.0%
!!	TCGTTGACTAAATTTAATTC+TGG	+	chr4.1:22132363-22132382	None:intergenic	25.0%
	AAAGAAGCAATTTCACACAA+AGG	-	chr4.1:22132632-22132651	MS.gene26402:CDS	30.0%
	AAGAAAGAACAAAGCAAGAA+TGG	-	chr4.1:22132771-22132790	MS.gene26402:CDS	30.0%
	AGAAAGAACAAAGCAAGAAT+GGG	-	chr4.1:22132772-22132791	MS.gene26402:CDS	30.0%
	ATACAACTTTGTTAGTTCCA+TGG	-	chr4.1:22131548-22131567	MS.gene26402:CDS	30.0%
	GAAAATTTGAACTTTGTTGG+TGG	-	chr4.1:22132197-22132216	MS.gene26402:CDS	30.0%
	GAAATGGTTCAATGAAGATA+AGG	-	chr4.1:22131890-22131909	MS.gene26402:intron	30.0%
	GATCAAAAACAGTGCATTTA+AGG	+	chr4.1:22132145-22132164	None:intergenic	30.0%
	GCTGTTCTTCAATATCTTTA+AGG	+	chr4.1:22132612-22132631	None:intergenic	30.0%
!	AAAACAGTGCATTTAAGGTT+AGG	+	chr4.1:22132140-22132159	None:intergenic	30.0%
!	TTTGAAACTTCATCCTTCTA+AGG	-	chr4.1:22131656-22131675	MS.gene26402:CDS	30.0%
	ACAATAGTGAAAGGCAAAGA+AGG	-	chr4.1:22131735-22131754	MS.gene26402:CDS	35.0%
	ACTGCAATTGGACTATGATT+TGG	-	chr4.1:22132724-22132743	MS.gene26402:CDS	35.0%
	AGAAACCGTTGTGTGTTAAA+AGG	+	chr4.1:22131707-22131726	None:intergenic	35.0%
	AGCAATTTCACACAAAGGAA+AGG	-	chr4.1:22132637-22132656	MS.gene26402:CDS	35.0%
	AGGATGAAGTTTCAAAGAAG+AGG	+	chr4.1:22131652-22131671	None:intergenic	35.0%
	ATCCAAGTTCTTTAGACATG+TGG	-	chr4.1:22131856-22131875	MS.gene26402:intron	35.0%
	ATTTCACACAAAGGAAAGGA+TGG	-	chr4.1:22132641-22132660	MS.gene26402:CDS	35.0%
	GAAAAACTCATATACGATGC+AGG	-	chr4.1:22132794-22132813	MS.gene26402:CDS	35.0%
	GAGTATGTTTCAAGATGCTA+TGG	-	chr4.1:22131989-22132008	MS.gene26402:intron	35.0%
	GCTAATTCAGAAAGAGTCAT+TGG	+	chr4.1:22131630-22131649	None:intergenic	35.0%
	GTCATTGTCAATATTGTGTC+CGG	+	chr4.1:22132333-22132352	None:intergenic	35.0%
	GTGATGCTTACAATGTTTCT+TGG	-	chr4.1:22132746-22132765	MS.gene26402:CDS	35.0%
	TACAAAATTACCCCCATTTC+TGG	-	chr4.1:22132827-22132846	MS.gene26402:CDS	35.0%
	TGGATTAACTGAACTGCAAT+TGG	-	chr4.1:22132712-22132731	MS.gene26402:CDS	35.0%
	TTCACTTTCTTCAGAACCAT+TGG	+	chr4.1:22131493-22131512	None:intergenic	35.0%
	TTCATTGAACCATTTCTCTG+AGG	+	chr4.1:22131886-22131905	None:intergenic	35.0%
!	TGTACAAATGGATTTGAGCA+TGG	+	chr4.1:22131521-22131540	None:intergenic	35.0%
!!	CAATTTTGGTCTATGAAGCA+CGG	-	chr4.1:22132295-22132314	MS.gene26402:CDS	35.0%
!!!	AAAGAGTCATTGGTTTTCCA+TGG	+	chr4.1:22131620-22131639	None:intergenic	35.0%
!!!	TGCTGATGCTGTTTTACTTA+AGG	-	chr4.1:22132244-22132263	MS.gene26402:CDS	35.0%
	AGAGACTTGAAGCCAGAAAT+GGG	+	chr4.1:22132842-22132861	None:intergenic	40.0%
	AGTAAAACAGCATCAGCAGA+AGG	+	chr4.1:22132242-22132261	None:intergenic	40.0%
	CCTTCAAAGCTTCTCATAAG+TGG	-	chr4.1:22131792-22131811	MS.gene26402:intron	40.0%
	CTCCACATGTCTAAAGAACT+TGG	+	chr4.1:22131861-22131880	None:intergenic	40.0%
	GTAAAACAGCATCAGCAGAA+GGG	+	chr4.1:22132241-22132260	None:intergenic	40.0%
	GTTAAGTTACCAACAACCTG+TGG	+	chr4.1:22132170-22132189	None:intergenic	40.0%
	TTGGAGTCTCTTGTTGATGT+TGG	-	chr4.1:22132071-22132090	MS.gene26402:intron	40.0%
	TTTGATCAACCACAGGTTGT+TGG	-	chr4.1:22132158-22132177	MS.gene26402:CDS	40.0%
!	CCACTTATGAGAAGCTTTGA+AGG	+	chr4.1:22131795-22131814	None:intergenic	40.0%
!	GTGTTAAAAGGCGCAAGAAT+CGG	+	chr4.1:22131695-22131714	None:intergenic	40.0%
!	TTGCGCCTTTTAACACACAA+CGG	-	chr4.1:22131699-22131718	MS.gene26402:CDS	40.0%
!!	CAGGTTGTTGGTAACTTAAC+TGG	-	chr4.1:22132170-22132189	MS.gene26402:CDS	40.0%
!!	CTTATGAGAAGCTTTGAAGG+AGG	+	chr4.1:22131792-22131811	None:intergenic	40.0%
!!!	CACTGTTTTTGATCAACCAC+AGG	-	chr4.1:22132151-22132170	MS.gene26402:CDS	40.0%
	ACACTGTTTGAGTGTGCAAC+AGG	-	chr4.1:22131918-22131937	MS.gene26402:intron	45.0%
	ACTGTTTGAGTGTGCAACAG+GGG	-	chr4.1:22131920-22131939	MS.gene26402:intron	45.0%
	AGACTTGAAGCCAGAAATGG+GGG	+	chr4.1:22132840-22132859	None:intergenic	45.0%
	ATCAGCTATGCCTAGTTCCA+TGG	+	chr4.1:22131586-22131605	None:intergenic	45.0%
	CACTGTTTGAGTGTGCAACA+GGG	-	chr4.1:22131919-22131938	MS.gene26402:intron	45.0%
	CTCAAATCAGCCATGGAACT+AGG	-	chr4.1:22131573-22131592	MS.gene26402:CDS	45.0%
	GAGACTTGAAGCCAGAAATG+GGG	+	chr4.1:22132841-22132860	None:intergenic	45.0%
	GAGAGACTTGAAGCCAGAAA+TGG	+	chr4.1:22132843-22132862	None:intergenic	45.0%
	GAGTCTCTTGTTGATGTTGG+AGG	-	chr4.1:22132074-22132093	MS.gene26402:intron	45.0%
	GATGAAACAAGTGACGATCG+TGG	-	chr4.1:22132692-22132711	MS.gene26402:CDS	45.0%
	GCTGATGCAATTCACAACCA+TGG	-	chr4.1:22131600-22131619	MS.gene26402:CDS	45.0%
	TCTCTTGTTGATGTTGGAGG+TGG	-	chr4.1:22132077-22132096	MS.gene26402:intron	45.0%
	TGTGGAGTTCCTCAGAGAAA+TGG	-	chr4.1:22131874-22131893	MS.gene26402:intron	45.0%
!	TACAGTGGGTTCTGCATGAT+TGG	-	chr4.1:22132573-22132592	MS.gene26402:CDS	45.0%
	AGAGAACAAACGCGTGTTCG+AGG	-	chr4.1:22132046-22132065	MS.gene26402:intron	50.0%
	CATGGCACTCAAATCAGCCA+TGG	-	chr4.1:22131566-22131585	MS.gene26402:CDS	50.0%
	CGAACACGCGTTTGTTCTCT+TGG	+	chr4.1:22132046-22132065	None:intergenic	50.0%
	GAGAACAAACGCGTGTTCGA+GGG	-	chr4.1:22132047-22132066	MS.gene26402:intron	50.0%
	GTTGATGTTGGAGGTGGTAC+TGG	-	chr4.1:22132083-22132102	MS.gene26402:intron	50.0%
!	TGCAACAGGGGAGAGCTTTT+GGG	-	chr4.1:22131932-22131951	MS.gene26402:intron	50.0%
!!	CATGGCTGATTTGAGTGCCA+TGG	+	chr4.1:22131568-22131587	None:intergenic	50.0%
	CAAACGCGTGTTCGAGGGTT+TGG	-	chr4.1:22132052-22132071	MS.gene26402:intron	55.0%
!	GATGTTGGAGGTGGTACTGG+TGG	-	chr4.1:22132086-22132105	MS.gene26402:intron	55.0%
!	GTGCAACAGGGGAGAGCTTT+TGG	-	chr4.1:22131931-22131950	MS.gene26402:intron	55.0%
	AGCACGGACACTGCACACAC+CGG	-	chr4.1:22132311-22132330	MS.gene26402:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	22131477	22132880	22131477	ID=MS.gene26402
chr4.1	mRNA	22131477	22132880	22131477	ID=MS.gene26402.t1;Parent=MS.gene26402
chr4.1	exon	22132092	22132880	22132092	ID=MS.gene26402.t1.exon1;Parent=MS.gene26402.t1
chr4.1	CDS	22132092	22132880	22132092	ID=cds.MS.gene26402.t1;Parent=MS.gene26402.t1
chr4.1	exon	22131477	22131779	22131477	ID=MS.gene26402.t1.exon2;Parent=MS.gene26402.t1
chr4.1	CDS	22131477	22131779	22131477	ID=cds.MS.gene26402.t1;Parent=MS.gene26402.t1

Gene Sequence

>MS.gene26402

ATGGCTTTCACTACCAATGGTTCTGAAGAAAGTGAACTGTACCATGCTCAAATCCATTTGTACAAACATGTATACAACTTTGTTAGTTCCATGGCACTCAAATCAGCCATGGAACTAGGCATAGCTGATGCAATTCACAACCATGGAAAACCAATGACTCTTTCTGAATTAGCCTCTTCTTTGAAACTTCATCCTTCTAAGGTTAATATACTTCACCGATTCTTGCGCCTTTTAACACACAACGGTTTCTTTGCAAAAACAATAGTGAAAGGCAAAGAAGGAGAAGAAGAAGAAATAGCATATTCTCTCACACCTCCTTCAAAGCTTCTCATAAGTGGAAAACCAACATGTTTATCTTCAATTGTTAAAGGAGCACTTCATCCAAGTTCTTTAGACATGTGGAGTTCCTCAGAGAAATGGTTCAATGAAGATAAGGAACAAACACTGTTTGAGTGTGCAACAGGGGAGAGCTTTTGGGATTTTCTTAACAAAGATTCTGAGTCTAGTACTTTGAGTATGTTTCAAGATGCTATGGCATCTGATTCTAGAATGTTTAAGCTTGTTCTCCAAGAGAACAAACGCGTGTTCGAGGGTTTGGAGTCTCTTGTTGATGTTGGAGGTGGTACTGGTGGTGTTACAAAACTCATTAATGAAATTTTTCCTAACCTTAAATGCACTGTTTTTGATCAACCACAGGTTGTTGGTAACTTAACTGGAAATGAAAATTTGAACTTTGTTGGTGGAGATATGTTCAAATCTATCCCTTCTGCTGATGCTGTTTTACTTAAGGTAAGCATGTTCAACAATTGTTACTATTTCAATTTTGGTCTATGAAGCACGGACACTGCACACACCGGACACAATATTGACAATGACATAGACACCAGAATTAAATTTAGTCAACGAAACTGTTTGAATTTAATAACATATGACTGCGTCGAGATAATGTTAGACATTAAAATGTGTAGGACACATTACCTTCAATCTTGTTATATAACTTTTACTAATAAGGATAAAATGGTAGTAACTAGTAACAAATTAAGATGCATATTTTGGATCAACCAAATAACTAAATTAAATGTCAATTGTCTTTTATTACAGTGGGTTCTGCATGATTGGAATGATGAACAATCCTTAAAGATATTGAAGAACAGCAAAGAAGCAATTTCACACAAAGGAAAGGATGGAAAAGTGATTATCATCGATATATCAATTGATGAAACAAGTGACGATCGTGGATTAACTGAACTGCAATTGGACTATGATTTGGTGATGCTTACAATGTTTCTTGGTAAAGAAAGAACAAAGCAAGAATGGGAAAAACTCATATACGATGCAGGTTTTAGCAGCTACAAAATTACCCCCATTTCTGGCTTCAAGTCTCTCATTGAAGTTTATCCTTAA

Protein sequence

>MS.gene26402.t1

MAFTTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASSLKLHPSKVNILHRFLRLLTHNGFFAKTIVKGKEGEEEEIAYSLTPPSKLLISGKPTCLSSIVKGALHPSSLDMWSSSEKWFNEDKEQTLFECATGESFWDFLNKDSESSTLSMFQDAMASDSRMFKLVLQENKRVFEGLESLVDVGGGTGGVTKLINEIFPNLKCTVFDQPQVVGNLTGNENLNFVGGDMFKSIPSADAVLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVIIIDISIDETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPISGFKSLIEVYP