AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27209

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27209.t1 MTR_2g023880 98.958 192 2 0 1 192 31 222 1.90e-136 380
MS.gene27209.t1 MTR_4g049540 60.733 191 74 1 1 191 31 220 3.76e-82 243
MS.gene27209.t1 MTR_2g088060 49.738 191 95 1 1 191 31 220 9.17e-55 173
MS.gene27209.t1 MTR_7g056133 43.094 181 95 2 2 175 33 212 6.66e-41 138
MS.gene27209.t1 MTR_5g093360 55.882 68 29 1 2 69 32 98 4.05e-22 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27209.t1 AT5G42570 62.011 179 67 1 1 179 31 208 1.21e-77 231
MS.gene27209.t1 AT1G11905 60.000 180 71 1 1 180 31 209 1.52e-72 219
MS.gene27209.t1 AT1G11905 55.556 180 78 2 1 180 31 208 9.55e-61 189
MS.gene27209.t1 AT5G48660 39.560 182 102 2 2 176 33 213 1.56e-34 122
MS.gene27209.t1 AT3G07190 39.011 182 103 2 2 176 33 213 4.93e-33 118
MS.gene27209.t1 AT3G20450 52.475 101 47 1 3 102 37 137 2.57e-28 103
MS.gene27209.t1 AT5G48660 37.226 137 79 1 47 176 1 137 9.83e-20 81.6

Find 43 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCTCACATATTCTTCT+TGG 0.219887 2.1:+65113175 None:intergenic
TCCTACCGATCAGGTTCTTA+TGG 0.252341 2.1:-65115542 MS.gene27209:CDS
GTAGCTGGTTGCGGAGGTTT+TGG 0.307160 2.1:+65113231 None:intergenic
CTATCATAAGTGCAAGAAAA+AGG 0.327998 2.1:+65113534 None:intergenic
GGGGCTGAGGATGCGAAAGC+TGG 0.347270 2.1:-65113437 MS.gene27209:CDS
GAAGCTTGTAATAATGGCTT+TGG 0.364769 2.1:-65115698 None:intergenic
CCATGCTCTTTCTCCGAATC+CGG 0.367578 2.1:+65113483 None:intergenic
AAGCCAGTGTAAGTGCTCTA+AGG 0.421670 2.1:-65113304 MS.gene27209:CDS
TTCTGTTAATCCTACCGATC+AGG 0.442382 2.1:-65115551 MS.gene27209:CDS
ACAGACTGCATCATTACATA+AGG 0.453424 2.1:-65113511 MS.gene27209:CDS
ACATAAGGGAACTCCGGATT+CGG 0.458900 2.1:-65113496 MS.gene27209:CDS
ACGAGGTCGTGGACCTGTTA+TGG 0.464283 2.1:-65115665 MS.gene27209:CDS
TTCTGTCAACAGATTGTAGC+TGG 0.470135 2.1:+65113216 None:intergenic
GAGAGCAGAAGTTGATCGTC+TGG 0.490422 2.1:-65113369 MS.gene27209:CDS
CAACAGATTGTAGCTGGTTG+CGG 0.507849 2.1:+65113222 None:intergenic
GTTATGGTGAAAACCGTTGC+TGG 0.514910 2.1:-65115649 MS.gene27209:CDS
TTCAGATGAAACTAAAACAA+TGG 0.522908 2.1:-65113411 MS.gene27209:CDS
GATCGGTTGAAACGAGGTCG+TGG 0.523142 2.1:-65115676 MS.gene27209:CDS
ACTTCAGTCAAAAGATAAAG+AGG 0.526615 2.1:-65113339 MS.gene27209:CDS
TGAATATGATCGCTTACTTG+AGG 0.542275 2.1:-65113258 MS.gene27209:CDS
TTGTAATAATGGCTTTGGAT+CGG 0.542465 2.1:-65115693 None:intergenic
AGCTGTTAAGAAACAAATGC+GGG 0.550293 2.1:-65113459 MS.gene27209:CDS
TCTTCTTGAAGCTACACTCA+TGG 0.550488 2.1:-65115512 MS.gene27209:CDS
CAGACTGCATCATTACATAA+GGG 0.555282 2.1:-65113510 MS.gene27209:CDS
CTTCTTGAAGCTACACTCAT+GGG 0.557141 2.1:-65115511 MS.gene27209:intron
CAGATTGTAGCTGGTTGCGG+AGG 0.567147 2.1:+65113225 None:intergenic
CAACAACGTGGGATCGAACA+TGG 0.569692 2.1:-65115577 MS.gene27209:CDS
GCTCTAAGGAAACAATCTGA+AGG 0.569864 2.1:-65113290 MS.gene27209:CDS
ATCATTACATAAGGGAACTC+CGG 0.575747 2.1:-65113502 MS.gene27209:CDS
GCCATAAGAACCTGATCGGT+AGG 0.586898 2.1:+65115541 None:intergenic
CTCTAAGGAAACAATCTGAA+GGG 0.587548 2.1:-65113289 MS.gene27209:CDS
CAGTCAAAAGATAAAGAGGT+AGG 0.596716 2.1:-65113335 MS.gene27209:CDS
CCGGATTCGGAGAAAGAGCA+TGG 0.597596 2.1:-65113483 MS.gene27209:CDS
GCTTTGGATCGGTTGAAACG+AGG 0.607033 2.1:-65115682 MS.gene27209:CDS
TTTCCTTAGAGCACTTACAC+TGG 0.609208 2.1:+65113301 None:intergenic
CTTAGCCATAAGAACCTGAT+CGG 0.613122 2.1:+65115537 None:intergenic
AACAAGAACAGTACCAGCAA+CGG 0.621936 2.1:+65115636 None:intergenic
CTGTTAAGAAACAAATGCGG+GGG 0.642686 2.1:-65113457 MS.gene27209:CDS
TGATGAAGATTCAACAACGT+GGG 0.655476 2.1:-65115588 MS.gene27209:CDS
GCTGTTAAGAAACAAATGCG+GGG 0.673517 2.1:-65113458 MS.gene27209:CDS
AAGCTGTTAAGAAACAAATG+CGG 0.709469 2.1:-65113460 MS.gene27209:CDS
TGTTAAGAAACAAATGCGGG+GGG 0.715839 2.1:-65113456 MS.gene27209:CDS
ATGATGAAGATTCAACAACG+TGG 0.740230 2.1:-65115589 MS.gene27209:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCATAATATGAAATAAAA+GGG + chr2.1:65114293-65114312 None:intergenic 10.0%
!! TATTCATAATATGAAATAAA+AGG + chr2.1:65114294-65114313 None:intergenic 10.0%
!! TTTAATAAGTCTAAATATTA+AGG + chr2.1:65115252-65115271 None:intergenic 10.0%
!! TATAGTATTAAACTTATTAG+TGG - chr2.1:65114374-65114393 MS.gene27209:intron 15.0%
!!! TTGAATTTGATTTATGTTTA+TGG - chr2.1:65113459-65113478 MS.gene27209:CDS 15.0%
!!! TTTTACTAGTTGTATATAAT+TGG - chr2.1:65114242-65114261 MS.gene27209:intron 15.0%
!!! TTTTGTAAATATGATCAAAT+AGG - chr2.1:65113558-65113577 MS.gene27209:intron 15.0%
!! AATCATAAGATATATGCATA+TGG + chr2.1:65114612-65114631 None:intergenic 20.0%
!! ATTGTTTATTGATGAGTTAT+TGG - chr2.1:65114946-65114965 MS.gene27209:intron 20.0%
!! TTCTAAACCATTGAAAAAAT+CGG + chr2.1:65114840-65114859 None:intergenic 20.0%
!! TTGATTTATGTTTATGGATT+TGG - chr2.1:65113465-65113484 MS.gene27209:CDS 20.0%
!!! AGTATTTCTTTTGAATAGTA+TGG - chr2.1:65114349-65114368 MS.gene27209:intron 20.0%
!!! ATGGGTATGTTTTTTTTAAA+TGG - chr2.1:65113373-65113392 MS.gene27209:CDS 20.0%
!!! ATTTTGCTTATAATTAAGAC+TGG - chr2.1:65113602-65113621 MS.gene27209:intron 20.0%
!!! CTATATGATTTGCAATTTTT+AGG - chr2.1:65115001-65115020 MS.gene27209:intron 20.0%
!!! GTTATTGTTTTACTATTGAT+AGG + chr2.1:65114222-65114241 None:intergenic 20.0%
!!! TCTTTGATTTTTAATAAGAC+CGG - chr2.1:65115138-65115157 MS.gene27209:intron 20.0%
!!! TGGGTATGTTTTTTTTAAAT+GGG - chr2.1:65113374-65113393 MS.gene27209:CDS 20.0%
! AAGTTTAACAACATACTAGA+TGG + chr2.1:65115050-65115069 None:intergenic 25.0%
! ACTACATTAATGAAAGGAAA+AGG + chr2.1:65113875-65113894 None:intergenic 25.0%
! AGATTGACTACATTAATGAA+AGG + chr2.1:65113881-65113900 None:intergenic 25.0%
! CTACATTAATGAAAGGAAAA+GGG + chr2.1:65113874-65113893 None:intergenic 25.0%
! TTATTAAAAGATATCCTCTG+TGG - chr2.1:65115264-65115283 MS.gene27209:intron 25.0%
! TTCAGATGAAACTAAAACAA+TGG - chr2.1:65115455-65115474 MS.gene27209:intron 25.0%
! TTTGGAAATATGTTGAACTT+AGG + chr2.1:65115097-65115116 None:intergenic 25.0%
!! AATCATTGTGATTCTTTTTC+AGG + chr2.1:65114558-65114577 None:intergenic 25.0%
!! GAATGAATGAATTTTGTATG+AGG - chr2.1:65113434-65113453 MS.gene27209:CDS 25.0%
!!! CAGCAATGTTTTGAAAAATT+TGG + chr2.1:65115115-65115134 None:intergenic 25.0%
!!! GAAGTTTTGAGTAATGTTTA+AGG - chr2.1:65114320-65114339 MS.gene27209:intron 25.0%
AAGCTGTTAAGAAACAAATG+CGG - chr2.1:65115406-65115425 MS.gene27209:intron 30.0%
AAGTTGAAAAATGTGAGCTT+TGG - chr2.1:65114900-65114919 MS.gene27209:intron 30.0%
ACAATGATTCAGCATTCATA+AGG - chr2.1:65114569-65114588 MS.gene27209:intron 30.0%
ACTTCAGTCAAAAGATAAAG+AGG - chr2.1:65115527-65115546 MS.gene27209:CDS 30.0%
AGATCTTCTCAATTTCCAAA+AGG + chr2.1:65113807-65113826 None:intergenic 30.0%
ATTCTTGGTCAATTATTTGC+AGG - chr2.1:65115303-65115322 MS.gene27209:intron 30.0%
CAGTATTGTTTATGTTGGAT+TGG + chr2.1:65115207-65115226 None:intergenic 30.0%
CTATCATAAGTGCAAGAAAA+AGG + chr2.1:65115335-65115354 None:intergenic 30.0%
CTCAACAGTATTGTTTATGT+TGG + chr2.1:65115212-65115231 None:intergenic 30.0%
GAATAACCCAACAAAGAATT+AGG + chr2.1:65114790-65114809 None:intergenic 30.0%
GTTTATTGATGAGTTATTGG+TGG - chr2.1:65114949-65114968 MS.gene27209:intron 30.0%
TGCTTATAATTAAGACTGGA+CGG - chr2.1:65113606-65113625 MS.gene27209:intron 30.0%
TGTTTGCCTAATTCTTTGTT+GGG - chr2.1:65114781-65114800 MS.gene27209:intron 30.0%
TTAAAACAACATAGCTGAAG+AGG - chr2.1:65115168-65115187 MS.gene27209:intron 30.0%
TTGTTTGCCTAATTCTTTGT+TGG - chr2.1:65114780-65114799 MS.gene27209:intron 30.0%
! ATGGTTTAGAATGAATGTTG+AGG - chr2.1:65114849-65114868 MS.gene27209:intron 30.0%
! TGATCAATCACAAAACCTTT+TGG - chr2.1:65113789-65113808 MS.gene27209:intron 30.0%
!! ACATTTGTAAGAGTGTTGTA+CGG - chr2.1:65113723-65113742 MS.gene27209:intron 30.0%
!!! ATGTTGTTTTAAGATGTGAC+CGG + chr2.1:65115160-65115179 None:intergenic 30.0%
!!! GATTCTTTTTCAGGAAAACA+CGG + chr2.1:65114549-65114568 None:intergenic 30.0%
!!! TTTGCTACCGATTTTTTCAA+TGG - chr2.1:65114830-65114849 MS.gene27209:intron 30.0%
AAGAACCATTCGAGAATTGA+AGG + chr2.1:65114015-65114034 None:intergenic 35.0%
ACAGACTGCATCATTACATA+AGG - chr2.1:65115355-65115374 MS.gene27209:intron 35.0%
ACTCCGAAAGATCAGAAAAT+GGG + chr2.1:65113845-65113864 None:intergenic 35.0%
AGCTGTTAAGAAACAAATGC+GGG - chr2.1:65115407-65115426 MS.gene27209:intron 35.0%
ATCATTACATAAGGGAACTC+CGG - chr2.1:65115364-65115383 MS.gene27209:intron 35.0%
ATGATGAAGATTCAACAACG+TGG - chr2.1:65113277-65113296 MS.gene27209:CDS 35.0%
CAGACTGCATCATTACATAA+GGG - chr2.1:65115356-65115375 MS.gene27209:intron 35.0%
CAGTCAAAAGATAAAGAGGT+AGG - chr2.1:65115531-65115550 MS.gene27209:CDS 35.0%
CTGTTGTCAATGCAGAAATA+CGG + chr2.1:65114435-65114454 None:intergenic 35.0%
GAGAATGTTCTACTTTCTTG+TGG - chr2.1:65114922-65114941 MS.gene27209:intron 35.0%
TGAATATGATCGCTTACTTG+AGG - chr2.1:65115608-65115627 MS.gene27209:CDS 35.0%
TGATGAAGATTCAACAACGT+GGG - chr2.1:65113278-65113297 MS.gene27209:CDS 35.0%
TTCAGCATTCATAAGGCATA+AGG - chr2.1:65114576-65114595 MS.gene27209:intron 35.0%
TTGCGAGCATTATTCATTCT+TGG - chr2.1:65115288-65115307 MS.gene27209:intron 35.0%
! CTCTAAGGAAACAATCTGAA+GGG - chr2.1:65115577-65115596 MS.gene27209:CDS 35.0%
! GAACCCATTTTCTGATCTTT+CGG - chr2.1:65113839-65113858 MS.gene27209:intron 35.0%
! GTCATATCTAGTTTCCGTAT+CGG + chr2.1:65113653-65113672 None:intergenic 35.0%
!!! TGGTATTTTAGCCTCCTAAT+GGG - chr2.1:65114682-65114701 MS.gene27209:intron 35.0%
!!! TTGGTATTTTAGCCTCCTAA+TGG - chr2.1:65114681-65114700 MS.gene27209:intron 35.0%
AACAAGAACAGTACCAGCAA+CGG + chr2.1:65113233-65113252 None:intergenic 40.0%
AATACACGTTCACGAGGTAT+AGG + chr2.1:65114521-65114540 None:intergenic 40.0%
AATCATATGAGCCCATTAGG+AGG + chr2.1:65114696-65114715 None:intergenic 40.0%
AATTAGGCAAACAACCAACG+AGG + chr2.1:65114774-65114793 None:intergenic 40.0%
CTGAATCATATGAGCCCATT+AGG + chr2.1:65114699-65114718 None:intergenic 40.0%
CTGTTAAGAAACAAATGCGG+GGG - chr2.1:65115409-65115428 MS.gene27209:intron 40.0%
CTTAGCCATAAGAACCTGAT+CGG + chr2.1:65113332-65113351 None:intergenic 40.0%
CTTCTTGAAGCTACACTCAT+GGG - chr2.1:65113355-65113374 MS.gene27209:CDS 40.0%
GACTCCGAAAGATCAGAAAA+TGG + chr2.1:65113846-65113865 None:intergenic 40.0%
GCTAAAATACCAAGAGTGAC+CGG + chr2.1:65114674-65114693 None:intergenic 40.0%
GCTGTTAAGAAACAAATGCG+GGG - chr2.1:65115408-65115427 MS.gene27209:intron 40.0%
GTTGTACGGATGTAATACAC+CGG - chr2.1:65113737-65113756 MS.gene27209:intron 40.0%
TCTTCTTGAAGCTACACTCA+TGG - chr2.1:65113354-65113373 MS.gene27209:CDS 40.0%
TGTTAAGAAACAAATGCGGG+GGG - chr2.1:65115410-65115429 MS.gene27209:intron 40.0%
TTCTGTCAACAGATTGTAGC+TGG + chr2.1:65115653-65115672 None:intergenic 40.0%
TTCTGTTAATCCTACCGATC+AGG - chr2.1:65113315-65113334 MS.gene27209:CDS 40.0%
! AGTTATTGGTGGCATTAGCA+GGG - chr2.1:65114960-65114979 MS.gene27209:intron 40.0%
! TTTCCTTAGAGCACTTACAC+TGG + chr2.1:65115568-65115587 None:intergenic 40.0%
!! GCTCTAAGGAAACAATCTGA+AGG - chr2.1:65115576-65115595 MS.gene27209:CDS 40.0%
AAGCCAGTGTAAGTGCTCTA+AGG - chr2.1:65115562-65115581 MS.gene27209:CDS 45.0%
ACATAAGGGAACTCCGGATT+CGG - chr2.1:65115370-65115389 MS.gene27209:intron 45.0%
AGAGGCTTTACCCTGTCAAA+CGG - chr2.1:65114070-65114089 MS.gene27209:intron 45.0%
CAACAGATTGTAGCTGGTTG+CGG + chr2.1:65115647-65115666 None:intergenic 45.0%
CGGAGCATAGCAGAAAAATC+AGG - chr2.1:65114141-65114160 MS.gene27209:intron 45.0%
CTGTCCTCTGTTGTCAAAAG+CGG - chr2.1:65114100-65114119 MS.gene27209:intron 45.0%
GTCAATGCAGAAATACGGTG+TGG + chr2.1:65114430-65114449 None:intergenic 45.0%
GTTATGGTGAAAACCGTTGC+TGG - chr2.1:65113217-65113236 MS.gene27209:CDS 45.0%
GTTCGAAATACACGTTCACG+AGG + chr2.1:65114527-65114546 None:intergenic 45.0%
TCCTACCGATCAGGTTCTTA+TGG - chr2.1:65113324-65113343 MS.gene27209:CDS 45.0%
TCTAGAAGCTGAACCTAGAC+AGG + chr2.1:65114462-65114481 None:intergenic 45.0%
TCTTGAGTTTACTGTGCCTC+TGG - chr2.1:65114031-65114050 MS.gene27209:intron 45.0%
TGTAATACACCGGTGTGTGT+TGG - chr2.1:65113747-65113766 MS.gene27209:intron 45.0%
TTTGACAGGGTAAAGCCTCT+CGG + chr2.1:65114070-65114089 None:intergenic 45.0%
! GAGTTATTGGTGGCATTAGC+AGG - chr2.1:65114959-65114978 MS.gene27209:intron 45.0%
! GCAACGGTTTTCACCATAAC+AGG + chr2.1:65113217-65113236 None:intergenic 45.0%
! TCTAGTTTCCGTATCGGATC+CGG + chr2.1:65113647-65113666 None:intergenic 45.0%
! TTTTGACAACAGAGGACAGG+AGG + chr2.1:65114099-65114118 None:intergenic 45.0%
AAATACGGTGTGGCGCATAG+CGG + chr2.1:65114420-65114439 None:intergenic 50.0%
ACTTGTGAGTGAAGCCTCGT+TGG - chr2.1:65114757-65114776 MS.gene27209:intron 50.0%
AGAAGTGGAAAGCGGCAAAG+CGG - chr2.1:65114195-65114214 MS.gene27209:intron 50.0%
AGAGAAAGAGCCTTCACCCT+TGG - chr2.1:65114643-65114662 MS.gene27209:intron 50.0%
ATAATGCTCGCAAGCCACAG+AGG + chr2.1:65115281-65115300 None:intergenic 50.0%
CAACAACGTGGGATCGAACA+TGG - chr2.1:65113289-65113308 MS.gene27209:CDS 50.0%
CCATGCTCTTTCTCCGAATC+CGG + chr2.1:65115386-65115405 None:intergenic 50.0%
CGGGTATCAGAAGTGGAAAG+CGG - chr2.1:65114187-65114206 MS.gene27209:intron 50.0%
GAGAAAGAGCCTTCACCCTT+GGG - chr2.1:65114644-65114663 MS.gene27209:intron 50.0%
GAGGCTTTACCCTGTCAAAC+GGG - chr2.1:65114071-65114090 MS.gene27209:intron 50.0%
GCCATAAGAACCTGATCGGT+AGG + chr2.1:65113328-65113347 None:intergenic 50.0%
GGCTGCCTTCAATTCTCGAA+TGG - chr2.1:65114007-65114026 MS.gene27209:intron 50.0%
TCTGCTATGCTCCGCTATAG+CGG + chr2.1:65114135-65114154 None:intergenic 50.0%
TGACAACAGAGCTCCTGTCT+AGG - chr2.1:65114446-65114465 MS.gene27209:intron 50.0%
TGACACTGACACAGACACGT+TGG - chr2.1:65113669-65113688 MS.gene27209:intron 50.0%
TTGCTGCATCTGATGCAGCA+TGG - chr2.1:65113986-65114005 MS.gene27209:intron 50.0%
TTGGGTAAACCGGTCACTCT+TGG - chr2.1:65114662-65114681 MS.gene27209:intron 50.0%
! AAGAGTGACCGGTTTACCCA+AGG + chr2.1:65114663-65114682 None:intergenic 50.0%
! ACGGAGTATCTGTCAAGCAC+CGG - chr2.1:65113625-65113644 MS.gene27209:intron 50.0%
! AGAGTGACCGGTTTACCCAA+GGG + chr2.1:65114662-65114681 None:intergenic 50.0%
! CGCTTTTGACAACAGAGGAC+AGG + chr2.1:65114102-65114121 None:intergenic 50.0%
! GAGAGCAGAAGTTGATCGTC+TGG - chr2.1:65115497-65115516 MS.gene27209:intron 50.0%
! GCTTTGGATCGGTTGAAACG+AGG - chr2.1:65113184-65113203 MS.gene27209:CDS 50.0%
AAAATCAGGACCACTGCGCC+AGG - chr2.1:65114155-65114174 MS.gene27209:intron 55.0%
AATACGGTGTGGCGCATAGC+GGG + chr2.1:65114419-65114438 None:intergenic 55.0%
ACGAGGTCGTGGACCTGTTA+TGG - chr2.1:65113201-65113220 MS.gene27209:CDS 55.0%
AGGGTAAAGCCTCTCGGACT+GGG + chr2.1:65114064-65114083 None:intergenic 55.0%
CAGATTGTAGCTGGTTGCGG+AGG + chr2.1:65115644-65115663 None:intergenic 55.0%
CCGGATTCGGAGAAAGAGCA+TGG - chr2.1:65115383-65115402 MS.gene27209:intron 55.0%
CGTTCACGAGGTATAGGCAC+TGG + chr2.1:65114515-65114534 None:intergenic 55.0%
GATCGGTTGAAACGAGGTCG+TGG - chr2.1:65113190-65113209 MS.gene27209:CDS 55.0%
GCCTTCACCCTTGGGTAAAC+CGG - chr2.1:65114652-65114671 MS.gene27209:intron 55.0%
TCTCCGTGACCAACACACAC+CGG + chr2.1:65113759-65113778 None:intergenic 55.0%
! ACACCGGTGTGTGTTGGTCA+CGG - chr2.1:65113753-65113772 MS.gene27209:intron 55.0%
! ACCGGTTTACCCAAGGGTGA+AGG + chr2.1:65114656-65114675 None:intergenic 55.0%
! GTAGCTGGTTGCGGAGGTTT+TGG + chr2.1:65115638-65115657 None:intergenic 55.0%
!! ACTGGAAGAGGTGCCAACAG+GGG + chr2.1:65114497-65114516 None:intergenic 55.0%
!! CACTGGAAGAGGTGCCAACA+GGG + chr2.1:65114498-65114517 None:intergenic 55.0%
!! CGAGGTATAGGCACTGGAAG+AGG + chr2.1:65114509-65114528 None:intergenic 55.0%
!! GTCAAGCACCGGATCCGATA+CGG - chr2.1:65113636-65113655 MS.gene27209:intron 55.0%
ACAGGAGGAGCCCGTTTGAC+AGG + chr2.1:65114084-65114103 None:intergenic 60.0%
ACCTCTCTCAGCTCCCCTGT+TGG - chr2.1:65114481-65114500 MS.gene27209:intron 60.0%
ACTTCTGATACCCGCCGACG+CGG + chr2.1:65114181-65114200 None:intergenic 60.0%
CAGGAGGAGCCCGTTTGACA+GGG + chr2.1:65114083-65114102 None:intergenic 60.0%
CAGGGTAAAGCCTCTCGGAC+TGG + chr2.1:65114065-65114084 None:intergenic 60.0%
CTATGCTCCGCTATAGCGGC+CGG + chr2.1:65114131-65114150 None:intergenic 60.0%
GAAACAAATGCGGGGGGCTG+AGG - chr2.1:65115416-65115435 MS.gene27209:intron 60.0%
GGGTAAAGCCTCTCGGACTG+GGG + chr2.1:65114063-65114082 None:intergenic 60.0%
GTTGTCAAAAGCGGCCGCAC+CGG - chr2.1:65114109-65114128 MS.gene27209:intron 60.0%
!! CATAGCGGGCGCTATTTCGG+TGG + chr2.1:65114405-65114424 None:intergenic 60.0%
!! GCACTGGAAGAGGTGCCAAC+AGG + chr2.1:65114499-65114518 None:intergenic 60.0%
!! GCGCATAGCGGGCGCTATTT+CGG + chr2.1:65114408-65114427 None:intergenic 60.0%
!!! GCGGCCGCTTTTGACAACAG+AGG + chr2.1:65114107-65114126 None:intergenic 60.0%
GCCAACAGGGGAGCTGAGAG+AGG + chr2.1:65114485-65114504 None:intergenic 65.0%
GGCTCTTTCCCCAGTCCGAG+AGG - chr2.1:65114052-65114071 MS.gene27209:intron 65.0%
GGGGCTGAGGATGCGAAAGC+TGG - chr2.1:65115429-65115448 MS.gene27209:intron 65.0%
! CGGACTGGGGAAAGAGCCAG+AGG + chr2.1:65114050-65114069 None:intergenic 65.0%
! GCGTCGGCGGGTATCAGAAG+TGG - chr2.1:65114180-65114199 MS.gene27209:intron 65.0%
CTCCGCTATAGCGGCCGGTG+CGG + chr2.1:65114126-65114145 None:intergenic 70.0%
ACCACTGCGCCAGGCCGCGT+CGG - chr2.1:65114164-65114183 MS.gene27209:intron 75.0%
TGATACCCGCCGACGCGGCC+TGG + chr2.1:65114176-65114195 None:intergenic 75.0%
! GGCCGCACCGGCCGCTATAG+CGG - chr2.1:65114121-65114140 MS.gene27209:intron 75.0%
! ACTGCGCCAGGCCGCGTCGG+CGG - chr2.1:65114167-65114186 MS.gene27209:intron 80.0%
! GCCGACGCGGCCTGGCGCAG+TGG + chr2.1:65114168-65114187 None:intergenic 85.0%
!! CTGCGCCAGGCCGCGTCGGC+GGG - chr2.1:65114168-65114187 MS.gene27209:intron 85.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 65113181 65115707 65113181 ID=MS.gene27209
chr2.1 mRNA 65113181 65115707 65113181 ID=MS.gene27209.t1;Parent=MS.gene27209
chr2.1 exon 65115512 65115707 65115512 ID=MS.gene27209.t1.exon1;Parent=MS.gene27209.t1
chr2.1 CDS 65115512 65115707 65115512 ID=cds.MS.gene27209.t1;Parent=MS.gene27209.t1
chr2.1 exon 65113181 65113563 65113181 ID=MS.gene27209.t1.exon2;Parent=MS.gene27209.t1
chr2.1 CDS 65113181 65113563 65113181 ID=cds.MS.gene27209.t1;Parent=MS.gene27209.t1
Gene Sequence

>MS.gene27209

ATGGCTTTGGATCGGTTGAAACGAGGTCGTGGACCTGTTATGGTGAAAACCGTTGCTGGTACTGTTCTTGTTGTTTTGTCTTCTAGTGTTTATAGTATGATGAAGATTCAACAACGTGGGATCGAACATGGTGCTTCTGTTAATCCTACCGATCAGGTTCTTATGGCTAAGCATCTTCTTGAAGCTACACTCATGGGTATGTTTTTTTTAAATGGGTTGTTGAAAAGTTTTGATTTTTGTTTATTTGCTTGATGAATGAATGAATTTTGTATGAGGAATTGAATTTGATTTATGTTTATGGATTTGGATTTAATGTGATTGCTCTGTTATGTGTAATTGTTTGCATTATTAATTTATTGAGTTTTTAGCATTATTATTTTTGTAAATATGATCAAATAGGTACATTTTTGTAATGTGTATAATTTTGCTTATAATTAAGACTGGACGGAGTATCTGTCAAGCACCGGATCCGATACGGAAACTAGATATGACACTGACACAGACACGTTGGAACAAAGAACGTTTTGTATGAGTTGTGAGATACATTTGTAAGAGTGTTGTACGGATGTAATACACCGGTGTGTGTTGGTCACGGAGATATGAATATATGATCAATCACAAAACCTTTTGGAAATTGAGAAGATCTTCTCGAATTCATGAACCCATTTTCTGATCTTTCGGAGTCATGTTCCCTTTTCCTTTCATTAATGTAGTCAATCTTGCATTGTGATGAATATGTTGTCTCTGTTGTTGCTGATGCAATTGATGAGTGATGATCTCTAGTGATCTAAAAGATTTGTTTGTTTTGCTGCATCTGATGCAGCATGGCTGCCTTCAATTCTCGAATGGTTCTTGAGTTTACTGTGCCTCTGGCTCTTTCCCCAGTCCGAGAGGCTTTACCCTGTCAAACGGGCTCCTCCTGTCCTCTGTTGTCAAAAGCGGCCGCACCGGCCGCTATAGCGGAGCATAGCAGAAAAATCAGGACCACTGCGCCAGGCCGCGTCGGCGGGTATCAGAAGTGGAAAGCGGCAAAGCGGACCTATCAATAGTAAAACAATAACTTTTACTAGTTGTATATAATTGGTATTAGCGATTTTCAATTTTGCTATCCCTTTTATTTCATATTATGAATACTTCATGAAGTTTTGAGTAATGTTTAAGGTATTTTAGTATTTCTTTTGAATAGTATGGTATATAGTATTAAACTTATTAGTGGCTGCACCACCGAAATAGCGCCCGCTATGCGCCACACCGTATTTCTGCATTGACAACAGAGCTCCTGTCTAGGTTCAGCTTCTAGACCTCTCTCAGCTCCCCTGTTGGCACCTCTTCCAGTGCCTATACCTCGTGAACGTGTATTTCGAACCGTGTTTTCCTGAAAAAGAATCACAATGATTCAGCATTCATAAGGCATAAGGATCATATAAACCATATGCATATATCTTATGATTGATTTAGTTAAAGAGAAAGAGCCTTCACCCTTGGGTAAACCGGTCACTCTTGGTATTTTAGCCTCCTAATGGGCTCATATGATTCAGACGCTCAACACTCATATAACTGAAGCTTCTAACTTGTAACTTGTGAGTGAAGCCTCGTTGGTTGTTTGCCTAATTCTTTGTTGGGTTATTCTGTAAATATTTGTGTTTCTTTTTGCTACCGATTTTTTCAATGGTTTAGAATGAATGTTGAGGCAACTTACATGAGATATGTGATCTTCTAAAGTTGAAAAATGTGAGCTTTGGAGAATGTTCTACTTTCTTGTGGTATTGTTTATTGATGAGTTATTGGTGGCATTAGCAGGGAGATTGAAATCAGATGTACTATATGATTTGCAATTTTTAGGATTCTCTCTCTCACAACTAGAAGCCATCTAGTATGTTGTTAAACTTTACAACTCAGATTGTATGTAATTTCCTAAGTTCAACATATTTCCAAATTTTTCAAAACATTGCTGTGTTCTTTGATTTTTAATAAGACCGGTCACATCTTAAAACAACATAGCTGAAGAGGTAAAGTTACAATTCCAATCCAACATAAACAATACTGTTGAGTTTCTGAACTGATTGTTCCTTAATATTTAGACTTATTAAAAGATATCCTCTGTGGCTTGCGAGCATTATTCATTCTTGGTCAATTATTTGCAGGTGCTATCCTTTTTCTTGCACTTATGATAGACAGACTGCATCATTACATAAGGGAACTCCGGATTCGGAGAAAGAGCATGGAAGCTGTTAAGAAACAAATGCGGGGGGCTGAGGATGCGAAAGCTGGAACTTCAGATGAAACTAAAACAATGGAGCAAGAAGCAGCTAAGTTGAGAGCAGAAGTTGATCGTCTGGAATCTGAACTTCAGTCAAAAGATAAAGAGGTAGGTGCTGCAGAAGCCAGTGTAAGTGCTCTAAGGAAACAATCTGAAGGGTTTCTTCTTGAATATGATCGCTTACTTGAGGAGAACCAAAACCTCCGCAACCAGCTACAATCTGTTGACAGAAGATTCTCACATTCAGTTTCCAAGAAGAATATGTGA
Protein sequence

>MS.gene27209.t1

MALDRLKRGRGPVMVKTVAGTVLVVLSSSVYSMMKIQQRGIEHGASVNPTDQVLMAKHLLEATLMGAILFLALMIDRLHHYIRELRIRRKSMEAVKKQMRGAEDAKAGTSDETKTMEQEAAKLRAEVDRLESELQSKDKEVGAAEASVSALRKQSEGFLLEYDRLLEENQNLRNQLQSVDRRFSHSVSKKNM